LOCUS       NM_003801               2054 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens glycosylphosphatidylinositol anchor attachment 1
            (GPAA1), mRNA.
ACCESSION   NM_003801
VERSION     NM_003801.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Kawaguchi,K., Yamamoto-Hino,M., Matsuyama,N., Suzuki,E. and Goto,S.
  TITLE     Subunits of the GPI transamidase complex localize to the
            endoplasmic reticulum and nuclear envelope in Drosophila
  JOURNAL   FEBS Lett 595 (7), 960-968 (2021)
   PUBMED   33496978
  REMARK    GeneRIF: Subunits of the GPI transamidase complex localize to the
            endoplasmic reticulum and nuclear envelope in Drosophila.
REFERENCE   3  (bases 1 to 2054)
  AUTHORS   Li,Y., Yang,L., Yang,J., Shi,J., Chai,P., Ge,S., Wang,Y., Fan,X.
            and Jia,R.
  TITLE     A novel variant in GPAA1, encoding a GPI transamidase complex
            protein, causes inherited vascular anomalies with various
            phenotypes
  JOURNAL   Hum Genet 139 (12), 1499-1511 (2020)
   PUBMED   32533362
  REMARK    GeneRIF: A novel variant in GPAA1, encoding a GPI transamidase
            complex protein, causes inherited vascular anomalies with various
            phenotypes.
REFERENCE   4  (bases 1 to 2054)
  AUTHORS   Su,C.T., Sinha,S., Eisenhaber,B. and Eisenhaber,F.
  TITLE     Structural modelling of the lumenal domain of human GPAA1, the
            metallo-peptide synthetase subunit of the transamidase complex,
            reveals zinc-binding mode and two flaps surrounding the active site
  JOURNAL   Biol Direct 15 (1), 14 (2020)
   PUBMED   32993792
  REMARK    GeneRIF: Structural modelling of the lumenal domain of human GPAA1,
            the metallo-peptide synthetase subunit of the transamidase complex,
            reveals zinc-binding mode and two flaps surrounding the active
            site.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2054)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 2054)
  AUTHORS   Ohishi,K., Inoue,N., Maeda,Y., Takeda,J., Riezman,H. and
            Kinoshita,T.
  TITLE     Gaa1p and gpi8p are components of a glycosylphosphatidylinositol
            (GPI) transamidase that mediates attachment of GPI to proteins
  JOURNAL   Mol Biol Cell 11 (5), 1523-1533 (2000)
   PUBMED   10793132
REFERENCE   7  (bases 1 to 2054)
  AUTHORS   Inoue,N., Ohishi,K., Endo,Y., Fujita,T., Takeda,J. and Kinoshita,T.
  TITLE     Human and mouse GPAA1 (Glycosylphosphatidylinositol anchor
            attachment 1) genes: genomic structures, chromosome loci and the
            presence of a minor class intron
  JOURNAL   Cytogenet Cell Genet 84 (3-4), 199-205 (1999)
   PUBMED   10393431
REFERENCE   8  (bases 1 to 2054)
  AUTHORS   Hiroi,Y., Komuro,I., Matsushita,I., Aburatani,H., Hosoda,T.,
            Nakahori,Y., Medof,M.E. and Yazaki,Y.
  TITLE     Assignment of the human GPAA1 gene, which encodes a product
            required for the attachment of glycosylphosphatidylinositols to
            proteins, at 8q24
  JOURNAL   Genomics 54 (2), 354-355 (1998)
   PUBMED   9828142
REFERENCE   9  (bases 1 to 2054)
  AUTHORS   Hiroi,Y., Komuro,I., Chen,R., Hosoda,T., Mizuno,T., Kudoh,S.,
            Georgescu,S.P., Medof,M.E. and Yazaki,Y.
  TITLE     Molecular cloning of human homolog of yeast GAA1 which is required
            for attachment of glycosylphosphatidylinositols to proteins
  JOURNAL   FEBS Lett 421 (3), 252-258 (1998)
   PUBMED   9468317
REFERENCE   10 (bases 1 to 2054)
  AUTHORS   Ploug,M., Ronne,E., Behrendt,N., Jensen,A.L., Blasi,F. and Dano,K.
  TITLE     Cellular receptor for urokinase plasminogen activator.
            Carboxyl-terminal processing and membrane anchoring by
            glycosyl-phosphatidylinositol
  JOURNAL   J Biol Chem 266 (3), 1926-1933 (1991)
   PUBMED   1846368
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC004129.2 and BC003171.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003801.3.
            
            Summary: Posttranslational glycosylphosphatidylinositol (GPI)
            anchor attachment serves as a general mechanism for linking
            proteins to the cell surface membrane. The protein encoded by this
            gene presumably functions in GPI anchoring at the GPI transfer
            step. The mRNA transcript is ubiquitously expressed in both fetal
            and adult tissues. The anchor attachment protein 1 contains an
            N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein
            kinase phosphorylation site, 1 leucine zipper pattern, 2 potential
            N-glycosylation sites, and 8 putative transmembrane domains.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.301001.1,
                                           SRR3476690.350575.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355091.9/ ENSP00000347206.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BC004129.2         14-24
            12-2054             BC003171.1         1-2043
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..2054
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /note="glycosylphosphatidylinositol anchor attachment 1"
                     /db_xref="GeneID:8733"
                     /db_xref="HGNC:HGNC:4446"
                     /db_xref="MIM:603048"
     exon            1..171
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..1963
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /note="glycophosphatidylinositol anchor attachment 1; GPI
                     transamidase subunit; anchor attachment protein 1 (Gaa1p,
                     yeast) homolog; GAA1 protein homolog; GPI anchor
                     attachment protein 1; GPAA1P anchor attachment protein 1
                     homolog; glycosylphosphatidylinositol anchor attachment
                     protein 1 homolog"
                     /codon_start=1
                     /product="glycosylphosphatidylinositol anchor attachment 1
                     protein"
                     /protein_id="NP_003792.1"
                     /db_xref="CCDS:CCDS43776.1"
                     /db_xref="GeneID:8733"
                     /db_xref="HGNC:HGNC:4446"
                     /db_xref="MIM:603048"
                     /translation="MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPL
                     TQRTYMSENAMGSTMVEEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLE
                     VYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAV
                     GLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGA
                     IQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPED
                     WTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVA
                     VGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALEL
                     WMQLHEAGMGLEEPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQH
                     VATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLAL
                     QLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGSLFLWRE
                     LQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLFWK"
     exon            172..351
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            352..463
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            464..611
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            612..713
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            714..910
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1107
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1261
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1357
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1548
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1719
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..2054
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2027..2032
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /note="hexamer: AATAAA"
     polyA_site      2054
                     /gene="GPAA1"
                     /gene_synonym="GAA1; GPIBD15; hGAA1"
                     /note="major polyA site"
ORIGIN      
        1 gagcgcgggt ccccgggtct gacaggagca gcctgtgggc accgcggcgg tagttggagg
       61 cgggagaggg tccgtagccg cgccgccctg ccccgccatg ggcctcctgt cggacccggt
      121 tcgccggcgc gcgctcgccc gcctagtgct gcgcctcaac gcgccgttgt gcgtgctgag
      181 ctacgtggcg ggcatcgcct ggttcttggc gctggttttc ccgccgctga cccagcgcac
      241 ttacatgtcg gagaacgcca tgggctccac catggtggag gagcagtttg cgggcggaga
      301 ccgtgcccgg gcttttgccc gggacttcgc cgcccaccgc aagaagtcgg gggctctgcc
      361 agtggcctgg cttgaacgga cgatgcggtc agtagggctg gaggtctaca cgcagagttt
      421 ctcccggaaa ctgcccttcc cagatgagac ccacgagcgc tatatggtgt cgggcaccaa
      481 cgtgtacggc atcctgcggg ccccgcgtgc tgccagcacc gagtcgcttg tgctcaccgt
      541 gccctgtggc tctgactcta ccaacagcca ggctgtgggg ctgctgctgg cactggctgc
      601 ccacttccgg gggcagattt attgggccaa agatatcgtc ttcctggtaa cagaacatga
      661 ccttctgggc actgaggctt ggcttgaagc ctaccacgat gtcaatgtca ctggcatgca
      721 gtcgtctccc ctgcagggcc gagctggggc cattcaggca gccgtggccc tggagctgag
      781 cagtgatgtg gtcaccagcc tcgatgtggc cgtggagggg cttaacgggc agctgcccaa
      841 ccttgacctg ctcaatctct tccagacctt ctgccagaaa gggggcctgt tgtgcacgct
      901 tcagggcaag ctgcagcccg aggactggac atcattggat ggaccgctgc agggcctgca
      961 gacactgctg ctcatggttc tgcggcaggc ctccggccgc ccccacggct cccatggcct
     1021 cttcctgcgc taccgtgtgg aggccctaac cctgcgtggc atcaatagct tccgccagta
     1081 caagtatgac ctggtggcag tgggcaaggc tttggagggc atgttccgca agctcaacca
     1141 cctcctggag cgcctgcacc agtccttctt cctctacttg ctccccggcc tctcccgctt
     1201 cgtctccatc ggcctctaca tgcccgctgt cggcttcttg ctcctggtcc ttggtctcaa
     1261 ggctctggaa ctgtggatgc agctgcatga ggctggaatg ggccttgagg agcccggggg
     1321 tgcccctggc cccagtgtac cccttccccc atcacagggt gtggggctgg cctcgctcgt
     1381 ggcacctctg ctgatctcac aggccatggg actggccctc tatgtcctgc cagtgctggg
     1441 ccaacacgtt gccacccagc acttcccagt ggcagaggct gaggctgtgg tgctgacact
     1501 gctggcgatt tatgcagctg gcctggccct gccccacaat acccaccggg tggtaagcac
     1561 acaggcccca gacaggggct ggatggcact gaagctggta gccctgatct acctagcact
     1621 gcagctgggc tgcatcgccc tcaccaactt ctcactgggc ttcctgctgg ccaccaccat
     1681 ggtgcccact gctgcgcttg ccaagcctca tgggccccgg accctctatg ctgccctgct
     1741 ggtgctgacc agcccggcag ccacgctcct tggcagcctg ttcctgtggc gggagctgca
     1801 ggaggcgcca ctgtcactgg ccgagggctg gcagctcttc ctggcagcgc tagcccaggg
     1861 tgtgctggag caccacacct acggcgccct gctcttccca ctgctgtccc tgggcctcta
     1921 cccctgctgg ctgcttttct ggaatgtgct cttctggaag tgagatctgc ctgtccgggc
     1981 tgggacagag actccccaag gaccccattc tgcctccttc tggggaaata aatgagtgtc
     2041 tgtttcagca gcta
//
