LOCUS       NM_001502               3337 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens glycoprotein 2 (GP2), transcript variant 2, mRNA.
ACCESSION   NM_001502 NM_016295
VERSION     NM_001502.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Stsiapanava,A., Xu,C., Nishio,S., Han,L., Yamakawa,N., Carroni,M.,
            Tunyasuvunakool,K., Jumper,J., de Sanctis,D., Wu,B. and Jovine,L.
  TITLE     Structure of the decoy module of human glycoprotein 2 and
            uromodulin and its interaction with bacterial adhesin FimH
  JOURNAL   Nat Struct Mol Biol 29 (3), 190-193 (2022)
   PUBMED   35273390
  REMARK    GeneRIF: Structure of the decoy module of human glycoprotein 2 and
            uromodulin and its interaction with bacterial adhesin FimH.
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Shpoliansky,M., Roggenbuck,D., Pinsker,M., Salamon,N., Weiss,B.,
            Shouval,D.S. and Werner,L.
  TITLE     Antibodies Against Glycoprotein 2 Are Specific Biomarkers for
            Pediatric Crohn's Disease
  JOURNAL   Dig Dis Sci 66 (8), 2619-2626 (2021)
   PUBMED   32886311
  REMARK    GeneRIF: Antibodies Against Glycoprotein 2 Are Specific Biomarkers
            for Pediatric Crohn's Disease.
REFERENCE   3  (bases 1 to 3337)
  AUTHORS   Zhang,T., Zhao,L., Wang,S., Liu,J., Chang,Y., Ma,L., Feng,J. and
            Niu,Y.
  TITLE     Common Variants in NUS1 and GP2 Genes Contributed to the Risk of
            Gestational Diabetes Mellitus
  JOURNAL   Front Endocrinol (Lausanne) 12, 685524 (2021)
   PUBMED   34326813
  REMARK    GeneRIF: Common Variants in NUS1 and GP2 Genes Contributed to the
            Risk of Gestational Diabetes Mellitus.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3337)
  AUTHORS   Lin,Y., Nakatochi,M., Hosono,Y., Ito,H., Kamatani,Y., Inoko,A.,
            Sakamoto,H., Kinoshita,F., Kobayashi,Y., Ishii,H., Ozaka,M.,
            Sasaki,T., Matsuyama,M., Sasahira,N., Morimoto,M., Kobayashi,S.,
            Fukushima,T., Ueno,M., Ohkawa,S., Egawa,N., Kuruma,S., Mori,M.,
            Nakao,H., Adachi,Y., Okuda,M., Osaki,T., Kamiya,S., Wang,C.,
            Hara,K., Shimizu,Y., Miyamoto,T., Hayashi,Y., Ebi,H., Kohmoto,T.,
            Imoto,I., Kasugai,Y., Murakami,Y., Akiyama,M., Ishigaki,K.,
            Matsuda,K., Hirata,M., Shimada,K., Okusaka,T., Kawaguchi,T.,
            Takahashi,M., Watanabe,Y., Kuriki,K., Kadota,A., Okada,R.,
            Mikami,H., Takezaki,T., Suzuki,S., Yamaji,T., Iwasaki,M.,
            Sawada,N., Goto,A., Kinoshita,K., Fuse,N., Katsuoka,F., Shimizu,A.,
            Nishizuka,S.S., Tanno,K., Suzuki,K., Okada,Y., Horikoshi,M.,
            Yamauchi,T., Kadowaki,T., Yu,H., Zhong,J., Amundadottir,L.T.,
            Doki,Y., Ishii,H., Eguchi,H., Bogumil,D., Haiman,C.A., Le
            Marchand,L., Mori,M., Risch,H., Setiawan,V.W., Tsugane,S.,
            Wakai,K., Yoshida,T., Matsuda,F., Kubo,M., Kikuchi,S. and Matsuo,K.
  TITLE     Genome-wide association meta-analysis identifies GP2 gene risk
            variants for pancreatic cancer
  JOURNAL   Nat Commun 11 (1), 3175 (2020)
   PUBMED   32581250
  REMARK    GeneRIF: Genome-wide association meta-analysis identifies GP2 gene
            risk variants for pancreatic cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3337)
  AUTHORS   Fukuoka,S.
  TITLE     Molecular cloning and sequences of cDNAs encoding alpha (large) and
            beta (small) isoforms of human pancreatic zymogen granule
            membrane-associated protein GP2
  JOURNAL   Biochim Biophys Acta 1491 (1-3), 376-380 (2000)
   PUBMED   10760606
REFERENCE   6  (bases 1 to 3337)
  AUTHORS   Wong,S.M. and Lowe,A.W.
  TITLE     Sequence of the cDNA encoding human GP-2, the major membrane
            protein in the secretory granule of the exocrine pancreas
  JOURNAL   Gene 171 (2), 311-312 (1996)
   PUBMED   8666297
REFERENCE   7  (bases 1 to 3337)
  AUTHORS   Jacob,M., Laine,J. and LeBel,D.
  TITLE     Specific interactions of pancreatic amylase at acidic pH. Amylase
            and the major protein of the zymogen granule membrane (GP-2) bind
            to immobilized or polymerized amylase
  JOURNAL   Biochem Cell Biol 70 (10-11), 1105-1114 (1992)
   PUBMED   1284286
REFERENCE   8  (bases 1 to 3337)
  AUTHORS   Fukuoka,S., Freedman,S.D., Yu,H., Sukhatme,V.P. and Scheele,G.A.
  TITLE     GP-2/THP gene family encodes self-binding
            glycosylphosphatidylinositol-anchored proteins in apical secretory
            compartments of pancreas and kidney
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1189-1193 (1992)
   PUBMED   1531535
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1992 Apr 15;89(8):3669]
REFERENCE   9  (bases 1 to 3337)
  AUTHORS   Paul,E., Leblond,F.A. and LeBel,D.
  TITLE     In resting conditions, the pancreatic granule membrane protein GP-2
            is secreted by cleavage of its glycosylphosphatidylinositol anchor
  JOURNAL   Biochem J 277 (Pt 3) (Pt 3), 879-881 (1991)
   PUBMED   1651706
REFERENCE   10 (bases 1 to 3337)
  AUTHORS   Fukuoka,S., Freedman,S.D. and Scheele,G.A.
  TITLE     A single gene encodes membrane-bound and free forms of GP-2, the
            major glycoprotein in pancreatic secretory (zymogen) granule
            membranes
  JOURNAL   Proc Natl Acad Sci U S A 88 (7), 2898-2902 (1991)
   PUBMED   2011597
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC106796.3.
            
            On Jun 2, 2019 this sequence version replaced NM_001502.3.
            
            Summary: This gene encodes an integral membrane protein that is
            secreted from intracellular zymogen granules and associates with
            the plasma membrane via glycosylphosphatidylinositol (GPI) linkage.
            The encoded protein binds pathogens such as enterobacteria, thereby
            playing an important role in the innate immune response. The
            C-terminus of this protein is related to the C-terminus of the
            protein encoded by the neighboring gene, uromodulin (UMOD).
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 1. The resulting
            protein (isoform 2, also known as alpha) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX648023.1, CR749600.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151405, SAMEA2153347
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302555.10/ ENSP00000304044.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                AC106796.3         94989-95035         c
            48-177              AC106796.3         93860-93989         c
            178-618             AC106796.3         91338-91778         c
            619-729             AC106796.3         90391-90501         c
            730-941             AC106796.3         87784-87995         c
            942-1090            AC106796.3         87142-87290         c
            1091-1336           AC106796.3         85707-85952         c
            1337-1499           AC106796.3         84735-84897         c
            1500-1584           AC106796.3         83478-83562         c
            1585-1629           AC106796.3         82179-82223         c
            1630-3337           AC106796.3         77096-78803         c
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.3"
     gene            1..3337
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /note="glycoprotein 2"
                     /db_xref="GeneID:2813"
                     /db_xref="HGNC:HGNC:4441"
                     /db_xref="MIM:602977"
     exon            1..47
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            48..177
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1688
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; pancreatic zymogen granule membrane associated
                     protein GP2; pancreatic secretory granule membrane major
                     glycoprotein GP2; glycoprotein 2 (zymogen granule
                     membrane)"
                     /codon_start=1
                     /product="pancreatic secretory granule membrane major
                     glycoprotein GP2 isoform 2 precursor"
                     /protein_id="NP_001493.2"
                     /db_xref="CCDS:CCDS10582.2"
                     /db_xref="GeneID:2813"
                     /db_xref="HGNC:HGNC:4441"
                     /db_xref="MIM:602977"
                     /translation="MPHLMERMVGSGLLWLALVSCILTQASAVQRGYGNPIEASSYGL
                     DLDCGAPGTPEAHVCFDPCQNYTLLDEPFRSTENSAGSQGCDKNMSGWYRFVGEGGVR
                     MSETCVQVHRCQTDAPMWLNGTHPALGDGITNHTACAHWSGNCCFWKTEVLVKACPGG
                     YHVYRLEGTPWCNLRYCTDPSTVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVH
                     SLQPQLDCGPREIKVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPV
                     QASACRNILERNQTHAIYKNTLSLVNDFIIRDTILNINFQCAYPLDMKVSLQAALQPI
                     VSSLNVSVDGNGEFIVRMALFQDQNYTNPYEGDAVELSVESVLYVGAILEQGDTSRFN
                     LVLRNCYATPTEDKADLVKYFIIRNSCSNQRDSTIHVEENGQSSESRFSVQMFMFAGH
                     YDLVFLHCEIHLCDSLNEQCQPSCSRSQVRSEVPAIDLARVLDLGPITRRGAQSPGVM
                     NGTPSTAGFLVAWPMVLLTVLLAWLF"
     exon            178..618
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            619..729
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            730..941
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1090
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1336
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1499
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1584
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1629
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..3337
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2401..2406
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /note="hexamer: AAGAAA"
     polyA_site      2422
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /note="major polyA site"
     regulatory      3313..3318
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GP2"
                     /gene_synonym="ZAP75"
                     /note="hexamer: ATTAAA"
     polyA_site      3337
                     /gene="GP2"
                     /gene_synonym="ZAP75"
ORIGIN      
        1 attcccatgg ctggccagag gaggaacgct ttgtgttctc atcggagctg catgggaagt
       61 ctgcatacag caaagtgacc tgcatgcctc accttatgga aaggatggtg ggctctggcc
      121 tcctgtggct ggccttggtc tcctgcattc tgacccaggc atctgcagtg cagcgaggtt
      181 atggaaaccc cattgaagcc agttcgtatg ggctggacct ggactgcgga gctcctggca
      241 ccccagaggc tcatgtctgt tttgacccct gtcagaatta caccctcctg gatgaaccct
      301 tccgaagcac agagaactca gcagggtccc aggggtgcga taaaaacatg agcggctggt
      361 accgctttgt aggggaagga ggagtaagga tgtcggagac ctgtgtccag gtgcaccgat
      421 gccagacaga cgctcccatg tggctgaatg ggacccaccc tgcccttggg gatggcatca
      481 ccaaccacac tgcctgtgcc cattggagtg gcaactgctg tttctggaaa acagaggtgc
      541 tggtgaaggc ctgcccaggc gggtaccatg tgtaccggtt ggaaggcact ccctggtgta
      601 atctgagata ctgcacagac ccatccactg tggaggacaa gtgtgagaag gcctgccgcc
      661 ccgaggagga gtgccttgcc ctcaacagca cctggggctg tttctgcaga caggacctca
      721 atagttctga tgtccacagt ttgcagcctc agctagactg tgggcccagg gagatcaagg
      781 tgaaggtgga caaatgtttg ctgggaggcc tgggtttggg ggaggaggtc attgcctacc
      841 tgcgagaccc aaactgcagc agcatcttgc agacagagga gaggaactgg gtatctgtga
      901 ccagccccgt ccaggctagt gcctgcagga acattctgga gagaaatcaa acccatgcca
      961 tctacaaaaa caccctctcc ttggtcaatg atttcatcat cagagacacc atcctcaaca
     1021 tcaacttcca atgtgcctac ccactggaca tgaaagtcag cctccaagct gccttgcagc
     1081 ccattgtaag ttccctgaac gtcagtgtgg acgggaatgg agagttcatt gtcaggatgg
     1141 ccctcttcca agaccagaac tacacgaatc cttacgaagg ggatgcagtt gaactgtctg
     1201 ttgagtccgt gctgtatgtg ggtgccatct tggaacaagg ggacacctcc cggtttaacc
     1261 tggtgttgag gaactgctat gccaccccca ctgaagacaa ggctgacctt gtgaagtatt
     1321 tcatcatcag aaacagctgc tcaaatcaac gtgattccac catccacgtg gaggagaatg
     1381 ggcagtcctc ggaaagccgg ttctcagttc agatgttcat gtttgctgga cattatgacc
     1441 tagttttcct gcattgtgag attcatctct gtgattctct taatgaacag tgccagcctt
     1501 cttgctcaag aagtcaagtc cgcagtgaag taccggccat cgacctagcc cgggttctag
     1561 atttggggcc catcactcgg agaggtgcac agtctcccgg tgtcatgaat ggaaccccta
     1621 gcactgcagg gttcctggtg gcctggccta tggtcctcct gactgtcctc ctggcttggc
     1681 tgttctgaga gctccgctga gcatctggcc ttgaagtttg tgttcttccc tctggcaatg
     1741 gctcccttca gcacttctgc tttccactcc aattcacaca ggcttggtat taatagaatc
     1801 aaggccaagc taggttagga aaagggaaga gctttcacct tctttaaaac tctcggctgg
     1861 gcgcagtggc tcatgcctgt aatcccagca ttttgggagg ctgaggcagg tggatcacct
     1921 gaggtcagca gttcaaaatc agcctggcca aaatgctgaa actccgtctc tactaaaaat
     1981 acaaaaatta gccaggcatg gtggcaggcg cctgtaatcc cagctactcg ggaggccaag
     2041 gcaggagaat tgctcgaact cagggggtgg aggttgcagt gagttgagat tgtgccattg
     2101 cactccagcc tgggcaacag agcaagactc tgtctcagga aaaaaaaaaa aaaaaaagaa
     2161 aagcaacata gtggggtttc tgtcaatctg tcctcggctg cccttctcat ttgttgatgg
     2221 gaccttgaaa gcaagcttgc taggtgccct ctgtggctcc agcctttacc ggaagtgtgg
     2281 tgcatgtttt taacttcagg gaagcggtat cctgtcactg gggtatggga tgagcatgga
     2341 gaagaggcac cagccacgat tccttcctaa gcatctcctg ttctgactgc tcatgaattg
     2401 aagaaactga cccttgtgtt cactctgctt cctctgattg tttctatcat ggtttccaaa
     2461 aattgccatg ggaacatgtc aaagggccac ttttggagac tctgctccag gaagaactag
     2521 tccccatagc tcccctatcc cccaccatac cacagacatg ctgtgactta gagaactaca
     2581 caaacatcct tgggacctag atgctggagg aatgacttaa tttgatgcag aaactccatc
     2641 acccaaggga gtacctctca ctgtaaacag tgctgtgctt gctctgaagg attaagcaag
     2701 aagtcccaac agaagcaatg caccagtcct gctatgggaa tgaaactgca aagcctagga
     2761 gatgggtgaa gtccctttct ggagaccttt aatgagtact tcaaagcacc cgacacagat
     2821 gcagcaaata gggcatcaca catacacagg cattaatact cacgggcata tagatgctga
     2881 catgtgtaca ctgacttacg cccttcccac agctacagat aaggcctcgc aaagttggcc
     2941 tcagagacac atcaggaacc aaggtggacc agcaggtgcc gagcctgtgt atctgcttgg
     3001 aggagacgtt ccaatgtgct gccttgttca gagatggtgt agttgcaaga aacagaaacc
     3061 caccacaatt tctcaggcaa aaagggagtt aattataagg acataagagc acaaagttcc
     3121 agtgcaagag atacatccag gctgcacaag ctccgggagt ggggcctggc aagccaaaag
     3181 aaaccaaagt ttgtcttgcc ttctgttcct ctttctgaag ccacatagcc ttttatgact
     3241 gtgtattttt gcatcgcttt tgttttcttt ttatgtctct gaagccagct tttcctgttc
     3301 actcatccct tgattaaata tggacattct agcttca
//
