LOCUS       NM_002080               2421 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens glutamic-oxaloacetic transaminase 2 (GOT2), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_002080
VERSION     NM_002080.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Prystupa,T.K., Sagan,D., Kocki,J., Kocki,T., Szymanowski,R. and
            Bogucki,J.
  TITLE     Surgical resection of lung cancer inhibits mRNA expression of GOT2
            gene encoding kynurenine aminotransferase in leukocytes
  JOURNAL   Ann Agric Environ Med 30 (4), 755-762 (2023)
   PUBMED   38153082
  REMARK    GeneRIF: Surgical resection of lung cancer inhibits mRNA expression
            of GOT2 gene encoding kynurenine aminotransferase in leukocytes.
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Broeks,M.H., Meijer,N.W.F., Westland,D., Bosma,M., Gerrits,J.,
            German,H.M., Ciapaite,J., van Karnebeek,C.D.M., Wanders,R.J.A.,
            Zwartkruis,F.J.T., Verhoeven-Duif,N.M. and Jans,J.J.M.
  TITLE     The malate-aspartate shuttle is important for de novo serine
            biosynthesis
  JOURNAL   Cell Rep 42 (9), 113043 (2023)
   PUBMED   37647199
REFERENCE   3  (bases 1 to 2421)
  AUTHORS   Liang,Q., Liu,S., Yin,F., Liu,M., Wang,L., Guo,E., Lei,L., Wu,L.,
            Yang,Y., Zhang,D. and Zeng,X.
  TITLE     Low expression of GOT2 promotes tumor progress and predicts poor
            prognosis in hepatocellular carcinoma
  JOURNAL   Biomark Med 17 (18), 755-765 (2023)
   PUBMED   38095985
  REMARK    GeneRIF: Low expression of GOT2 promotes tumor progress and
            predicts poor prognosis in hepatocellular carcinoma.
REFERENCE   4  (bases 1 to 2421)
  AUTHORS   Li,Y., Li,B., Xu,Y., Qian,L., Xu,T., Meng,G., Li,H., Wang,Y.,
            Zhang,L., Jiang,X., Liu,Q., Xie,Y., Cheng,C., Sun,B. and Yu,D.
  TITLE     GOT2 Silencing Promotes Reprogramming of Glutamine Metabolism and
            Sensitizes Hepatocellular Carcinoma to Glutaminase Inhibitors
  JOURNAL   Cancer Res 82 (18), 3223-3235 (2022)
   PUBMED   35895805
  REMARK    GeneRIF: GOT2 Silencing Promotes Reprogramming of Glutamine
            Metabolism and Sensitizes Hepatocellular Carcinoma to Glutaminase
            Inhibitors.
REFERENCE   5  (bases 1 to 2421)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   6  (bases 1 to 2421)
  AUTHORS   Watazu,Y., Uji,Y., Sugiuchi,H., Okabe,H. and Murao,S.
  TITLE     New automated measurement of mitochondrial aspartate
            aminotransferase with use of protease 401
  JOURNAL   Clin Chem 36 (4), 687-689 (1990)
   PUBMED   2182221
REFERENCE   7  (bases 1 to 2421)
  AUTHORS   Panteghini,M., Pagani,F. and Cuccia,C.
  TITLE     Effects of therapeutic coronary reperfusion on aspartate
            aminotransferase isoenzymes in sera of patients with acute
            myocardial infarction
  JOURNAL   Clin Chem 35 (6), 909-912 (1989)
   PUBMED   2731362
REFERENCE   8  (bases 1 to 2421)
  AUTHORS   Schiele,F., Artur,Y., Varasteh,A., Wellman,M. and Siest,G.
  TITLE     Serum mitochondrial aspartate aminotransferase activity: not useful
            as a marker of excessive alcohol consumption in an unselected
            population
  JOURNAL   Clin Chem 35 (6), 926-930 (1989)
   PUBMED   2567216
REFERENCE   9  (bases 1 to 2421)
  AUTHORS   Furuya,E., Yoshida,Y. and Tagawa,K.
  TITLE     Interaction of mitochondrial aspartate aminotransferase with
            negatively charged lecithin liposomes
  JOURNAL   J Biochem 85 (5), 1157-1163 (1979)
   PUBMED   376500
REFERENCE   10 (bases 1 to 2421)
  AUTHORS   Craig,I.W., Tolley,E., Bobrow,M. and van Heyningen,V.
  TITLE     Assignment of a gene necessary for the expression of mitochondrial
            glutamic-oxaloacetic transaminase in human-mouse hybrid cells
  JOURNAL   Cytogenet Cell Genet 22 (1-6), 190-194 (1978)
   PUBMED   752471
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB485399.1, BC000525.2 and
            AC012183.9.
            
            On Nov 23, 2018 this sequence version replaced NM_002080.3.
            
            Summary: Glutamic-oxaloacetic transaminase is a pyridoxal
            phosphate-dependent enzyme which exists in cytoplasmic and
            inner-membrane mitochondrial forms, GOT1 and GOT2, respectively.
            GOT plays a role in amino acid metabolism and the urea and
            tricarboxylic acid cycles. The two enzymes are homodimeric and show
            close homology. Two transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Oct 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.64324.1,
                                           SRR1803616.20421.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000245206.10/
                                                  ENSP00000245206.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                DB485399.1         37-72
            37-1370             BC000525.2         1-1334
            1371-1371           AC012183.9         133953-133953
            1372-2421           BC000525.2         1336-2385
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q21"
     gene            1..2421
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /note="glutamic-oxaloacetic transaminase 2"
                     /db_xref="GeneID:2806"
                     /db_xref="HGNC:HGNC:4433"
                     /db_xref="MIM:138150"
     exon            1..177
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1381
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /EC_number="2.6.1.1"
                     /EC_number="2.6.1.7"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; kynurenine aminotransferase IV; aspartate
                     aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT;
                     transaminase A; fatty acid-binding protein; glutamate
                     oxaloacetate transaminase 2; plasma membrane-associated
                     fatty acid-binding protein; kynurenine aminotransferase 4;
                     kynurenine--oxoglutarate transaminase 4;
                     kynurenine--oxoglutarate transaminase IV; aspartate
                     transaminase 2; aspartate aminotransferase 2;
                     glutamic-oxaloacetic transaminase 2, mitochondrial"
                     /codon_start=1
                     /product="aspartate aminotransferase, mitochondrial
                     isoform 1 precursor"
                     /protein_id="NP_002071.2"
                     /db_xref="CCDS:CCDS10801.1"
                     /db_xref="GeneID:2806"
                     /db_xref="HGNC:HGNC:4433"
                     /db_xref="MIM:138150"
                     /translation="MALLHSGRVLPGIAAAFHPGLAAAASARASSWWTHVEMGPPDPI
                     LGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGL
                     AEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKP
                     TWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNPTG
                     VDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQS
                     YAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTP
                     DLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVE
                     RLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK"
     exon            178..334
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            335..463
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            464..523
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            524..685
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            686..790
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            791..941
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1107
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1258
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..2421
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2400..2405
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /note="hexamer: AATAAA"
     polyA_site      2421
                     /gene="GOT2"
                     /gene_synonym="DEE82; KAT4; KATIV; KYAT4; mitAAT"
                     /note="major polyA site"
ORIGIN      
        1 accttcagca ggagccggtt ccctgtgtgt gtgtccgctc gccctctgct ccgtcctgcg
       61 gctgcccact gccctcctac ggtccaccat ggccctgctg cactccggcc gcgtcctccc
      121 cgggatcgcc gccgccttcc acccgggcct cgccgccgcg gcctctgcca gagccagctc
      181 ctggtggacc catgtggaaa tgggacctcc agatcccatt ctgggagtca ctgaagcctt
      241 taagagggac accaatagca aaaagatgaa tctgggagtt ggtgcctacc gggatgataa
      301 tggaaagcct tacgttctgc ctagcgtccg caaggcagag gcccagattg ccgcaaaaaa
      361 tttggacaag gaatacctgc ccattggggg actggctgaa ttttgcaagg catctgcaga
      421 actagccctg ggtgagaaca gcgaagtctt gaagagtggc cggtttgtca ctgtgcagac
      481 catttctgga actggagcct taaggatcgg agccagtttt ctgcaaagat tttttaagtt
      541 cagccgagat gtctttctgc ccaaaccaac ctggggaaac cacacaccca tcttcaggga
      601 tgctggcatg cagctacaag gttatcggta ttatgacccc aagacttgcg gttttgactt
      661 cacaggcgct gtggaggata tttcaaaaat accagagcag agtgttcttc ttctgcatgc
      721 ctgcgcccac aatcccacgg gagtggaccc gcgtccggaa cagtggaagg aaatagcaac
      781 agtggtgaag aaaaggaatc tctttgcgtt ctttgacatg gcctaccaag gctttgccag
      841 tggtgatggt gataaggatg cctgggctgt gcgccacttc atcgaacagg gcattaatgt
      901 ttgcctctgc caatcatatg ccaagaacat gggcttatat ggtgagcgtg taggagcctt
      961 cactatggtc tgcaaagatg cggatgaagc caaaagggta gagtcacagt tgaagatctt
     1021 gatccgtccc atgtattcca accctcccct caatggggcc cggattgctg ctgccattct
     1081 gaacacccca gatttgcgaa aacaatggct gcaagaagtg aaagtcatgg ctgaccgcat
     1141 cattggcatg cggactcaac tggtctccaa cctcaagaag gagggttcca cccacaattg
     1201 gcaacacatc accgaccaaa ttggcatgtt ctgtttcaca gggctaaagc ctgaacaggt
     1261 ggagcggctg atcaaggagt tctccatcta catgacaaaa gatggccgca tctctgtggc
     1321 aggggtcacc tccagcaacg tgggctacct tgcccatgcc attcaccagg tcaccaagta
     1381 atgtccctgg tgcgaggaaa cagagacaac ctttctgtct tcagcctctg ctattgagag
     1441 cttcacacag acaatgagag agggtggatg gtggtgagtg gatcatttct ttcagccaca
     1501 gtgtgtaaca ctcagcattt gaatgtttct cagaaaagaa catgtagtga cacagggcag
     1561 aggcatccat ggctggcgtc tggaatatta aaccaaactc tccccggtcc ttttttctcc
     1621 aacttttctc aaagagttta catgtgcaag aaagtcatcg caccaaaaaa cctgtcaatt
     1681 atgccattgc aatatttcag aagctttaac tgaagtgtca ggttcctcgt gagaaacagc
     1741 acacgttaga ggctttgaga gaaggcctag ttctgtcatg agtagtcggc ctcgtgtctg
     1801 tcctcccatc ttggaacaac cttatcaaca ggccgcactg cagaaatgat gttttatgaa
     1861 aaccaatgag gctgctgcca ctccagcaag ggaaataatg cagtttcctg tcttatttaa
     1921 gaaaaagaga aggctctctt ttctcccttg tcattgccgt tcttttcctt acacgcaaag
     1981 attttttaac tattgcagat tttcatccca ttctactgct tgattgacca tcaactccat
     2041 cctatcgaga tttatttaag aatgaagaac ataattttct gctgatgctg taccctcacc
     2101 cttttcagca aagaatagtg gagagtagga aactgtactt tatctcggca tcctcttgaa
     2161 tgatagtgca agtttctcca gttgggatgt tgtctctgcc cggttggacc tcctcccttt
     2221 gttgaatgtg gtgtgcagcc tctcatctca cactgtgagt ccagcggcgc agggtggtac
     2281 caggaaagag gatattctag gctttgcgtg ctgctagctg ggttcaggct tcacccactg
     2341 gaaagaacca ccatctgctc taaccatgta gacttattgc ggcctggttt ctctgttaca
     2401 ataaaattac tgtagaccca a
//
