LOCUS       NM_002079               1978 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.
ACCESSION   NM_002079
VERSION     NM_002079.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Zhang,C., Xu,Q., Xu,C., Yang,K., Xia,T., Hasi,W., Hao,M. and
            Kuang,H.
  TITLE     Sex Differences in the Association Between AST/ALT and Incidence of
            Type 2 Diabetes in Japanese Patients with Nonalcoholic Fatty Liver
            Disease: A Retrospective Cohort Study
  JOURNAL   Endocr Res 49 (1), 1-11 (2024)
   PUBMED   37752709
  REMARK    GeneRIF: Sex Differences in the Association Between AST/ALT and
            Incidence of Type 2 Diabetes in Japanese Patients with Nonalcoholic
            Fatty Liver Disease: A Retrospective Cohort Study.
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Broeks,M.H., Meijer,N.W.F., Westland,D., Bosma,M., Gerrits,J.,
            German,H.M., Ciapaite,J., van Karnebeek,C.D.M., Wanders,R.J.A.,
            Zwartkruis,F.J.T., Verhoeven-Duif,N.M. and Jans,J.J.M.
  TITLE     The malate-aspartate shuttle is important for de novo serine
            biosynthesis
  JOURNAL   Cell Rep 42 (9), 113043 (2023)
   PUBMED   37647199
REFERENCE   3  (bases 1 to 1978)
  AUTHORS   Guo,Y., Chen,T., Liang,X., Gou,S., Xiong,J., Cui,J. and Peng,T.
  TITLE     Tumor Cell Derived Exosomal GOT1 Suppresses Tumor Cell Ferroptosis
            to Accelerate Pancreatic Cancer Progression by Activating Nrf2/HO-1
            Axis via Upregulating CCR2 Expression
  JOURNAL   Cells 11 (23), 3893 (2022)
   PUBMED   36497150
  REMARK    GeneRIF: Tumor Cell Derived Exosomal GOT1 Suppresses Tumor Cell
            Ferroptosis to Accelerate Pancreatic Cancer Progression by
            Activating Nrf2/HO-1 Axis via Upregulating CCR2 Expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1978)
  AUTHORS   Saxena,S., Achyuth,B. S, Murthy,T.P.K., Chandramohan,V., Yadav,A.K.
            and Singh,T.R.
  TITLE     Structural and functional analysis of disease-associated mutations
            in GOT1 gene: An in silico study
  JOURNAL   Comput Biol Med 136, 104695 (2021)
   PUBMED   34352456
  REMARK    GeneRIF: Structural and functional analysis of disease-associated
            mutations in GOT1 gene: An in silico study.
REFERENCE   5  (bases 1 to 1978)
  AUTHORS   Kremer,D.M., Nelson,B.S., Lin,L., Yarosz,E.L., Halbrook,C.J.,
            Kerk,S.A., Sajjakulnukit,P., Myers,A., Thurston,G., Hou,S.W.,
            Carpenter,E.S., Andren,A.C., Nwosu,Z.C., Cusmano,N., Wisner,S.,
            Mbah,N.E., Shan,M., Das,N.K., Magnuson,B., Little,A.C.,
            Savani,M.R., Ramos,J., Gao,T., Sastra,S.A., Palermo,C.F.,
            Badgley,M.A., Zhang,L., Asara,J.M., McBrayer,S.K., di
            Magliano,M.P., Crawford,H.C., Shah,Y.M., Olive,K.P. and
            Lyssiotis,C.A.
  TITLE     GOT1 inhibition promotes pancreatic cancer cell death by
            ferroptosis
  JOURNAL   Nat Commun 12 (1), 4860 (2021)
   PUBMED   34381026
  REMARK    GeneRIF: GOT1 inhibition promotes pancreatic cancer cell death by
            ferroptosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1978)
  AUTHORS   Doyle,J.M., Schinina,M.E., Bossa,F. and Doonan,S.
  TITLE     The amino acid sequence of cytosolic aspartate aminotransferase
            from human liver
  JOURNAL   Biochem J 270 (3), 651-657 (1990)
   PUBMED   2241899
REFERENCE   7  (bases 1 to 1978)
  AUTHORS   Panteghini,M.
  TITLE     Aspartate aminotransferase isoenzymes
  JOURNAL   Clin Biochem 23 (4), 311-319 (1990)
   PUBMED   2225456
  REMARK    Review article
REFERENCE   8  (bases 1 to 1978)
  AUTHORS   Bousquet-Lemercier,B., Pol,S., Pave-Preux,M., Hanoune,J. and
            Barouki,R.
  TITLE     Properties of human liver cytosolic aspartate aminotransferase
            mRNAs generated by alternative polyadenylation site selection
  JOURNAL   Biochemistry 29 (22), 5293-5299 (1990)
   PUBMED   1974457
REFERENCE   9  (bases 1 to 1978)
  AUTHORS   Watazu,Y., Uji,Y., Sugiuchi,H., Okabe,H. and Murao,S.
  TITLE     New automated measurement of mitochondrial aspartate
            aminotransferase with use of protease 401
  JOURNAL   Clin Chem 36 (4), 687-689 (1990)
   PUBMED   2182221
REFERENCE   10 (bases 1 to 1978)
  AUTHORS   Kamei,S., Ohkubo,A. and Yamanaka,M.
  TITLE     Apoenzyme of aspartate aminotransferase isozymes in serum and its
            diagnostic usefullness for hepatic diseases
  JOURNAL   Clin Chim Acta 96 (1-2), 97-105 (1979)
   PUBMED   225064
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA581933.1, AL391684.6 and
            EB387028.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002079.2.
            
            Summary: Glutamic-oxaloacetic transaminase is a pyridoxal
            phosphate-dependent enzyme which exists in cytoplasmic and
            mitochondrial forms, GOT1 and GOT2, respectively.  GOT plays a role
            in amino acid metabolism and the urea and tricarboxylic acid
            cycles. The two enzymes are homodimeric and show close homology.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005609.1, SRR3476690.265247.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370508.7/ ENSP00000359539.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-417               DA581933.1         150-566
            418-483             AL391684.6         93759-93824         c
            484-596             AL391684.6         93170-93282         c
            597-701             AL391684.6         92789-92893         c
            702-852             AL391684.6         90757-90907         c
            853-1018            AL391684.6         90502-90667         c
            1019-1161           AL391684.6         89613-89755         c
            1162-1947           AL391684.6         83934-84719         c
            1948-1978           EB387028.1         371-401
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.2"
     gene            1..1978
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /note="glutamic-oxaloacetic transaminase 1"
                     /db_xref="GeneID:2805"
                     /db_xref="HGNC:HGNC:4432"
                     /db_xref="MIM:138180"
     exon            1..177
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1301
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /EC_number="2.6.1.1"
                     /EC_number="2.6.1.3"
                     /note="growth-inhibiting protein 18; transaminase A;
                     glutamate oxaloacetate transaminase 1; cysteine
                     transaminase, cytoplasmic; cysteine aminotransferase,
                     cytoplasmic; aspartate transaminase 1;
                     glutamic-oxaloacetic transaminase 1, soluble; aspartate
                     aminotransferase 1; testis secretory sperm-binding protein
                     Li 196a"
                     /codon_start=1
                     /product="aspartate aminotransferase, cytoplasmic"
                     /protein_id="NP_002070.1"
                     /db_xref="CCDS:CCDS7479.1"
                     /db_xref="GeneID:2805"
                     /db_xref="HGNC:HGNC:4432"
                     /db_xref="MIM:138180"
                     /translation="MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTD
                     DCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVG
                     GVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYR
                     YWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFL
                     FPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGK
                     EPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTM
                     RSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSG
                     LTTKNLDYVATSIHEAVTKIQ"
     exon            178..359
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            360..483
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            484..596
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            597..701
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            702..852
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            853..1018
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1161
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1978
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1538..1543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /note="hexamer: AATAAA"
     polyA_site      1563
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /note="major polyA site"
     regulatory      1712..1717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /note="hexamer: ATTAAA"
     polyA_site      1736
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
     regulatory      1959..1964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /note="hexamer: AATAAA"
     polyA_site      1978
                     /gene="GOT1"
                     /gene_synonym="AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18;
                     SGOT"
                     /note="major polyA site"
ORIGIN      
        1 agacctgacc tgccagctcc gggcgtgggg tgaaatctct tgattcctag tctctcgata
       61 tggcacctcc gtcagtcttt gccgaggttc cgcaggccca gcctgtcctg gtcttcaagc
      121 tcactgccga cttcagggag gatccggacc cccgcaaggt caacctggga gtgggagcat
      181 atcgcacgga tgactgccat ccctgggttt tgccagtagt gaagaaagtg gagcagaaga
      241 ttgctaatga caatagccta aatcacgagt atctgccaat cctgggcctg gctgagttcc
      301 ggagctgtgc ttctcgtctt gcccttgggg atgacagccc agcactcaag gagaagcggg
      361 taggaggtgt gcaatctttg gggggaacag gtgcacttcg aattggagct gatttcttag
      421 cgcgttggta caatggaaca aacaacaaga acacacctgt ctatgtgtcc tcaccaacct
      481 gggagaatca caatgctgtg ttttccgctg ctggttttaa agacattcgg tcctatcgct
      541 actgggatgc agagaagaga ggattggacc tccagggctt cctgaatgat ctggagaatg
      601 ctcctgagtt ctccattgtt gtcctccacg cctgtgcaca caacccaact gggattgacc
      661 caactccgga gcagtggaag cagattgctt ctgtcatgaa gcaccggttt ctgttcccct
      721 tctttgactc agcctatcag ggcttcgcat ctggaaacct ggagagagat gcctgggcca
      781 ttcgctattt tgtgtctgaa ggcttcgagt tcttctgtgc ccagtccttc tccaagaact
      841 tcgggctcta caatgagaga gtcgggaatc tgactgtggt tggaaaagaa cctgagagca
      901 tcctgcaagt cctttcccag atggagaaga tcgtgcggat tacttggtcc aatccccccg
      961 cccagggagc acgaattgtg gccagcaccc tctctaaccc tgagctcttt gaggaatgga
     1021 caggtaatgt gaagacaatg gctgaccgga ttctgaccat gagatctgaa ctcagggcac
     1081 gactagaagc cctcaaaacc cctgggacct ggaaccacat cactgatcaa attggcatgt
     1141 tcagcttcac tgggttgaac cccaagcagg ttgagtatct ggtcaatgaa aagcacatct
     1201 acctgctgcc aagtggtcga atcaacgtga gtggcttaac caccaaaaat ctagattacg
     1261 tggccacctc catccatgaa gcagtcacca aaatccagtg aagaaacacc acccgtccag
     1321 taccaccaaa gtagttctct gtcatgtgtg ttccctgcct gcacaaacct acatgtacat
     1381 accatggatt agagacactt gcaggactga aaggctgctc tggtgaggca gcctctgttt
     1441 aaaccggccc cacatgaaga gaacatccct tgagacgaat ttggagactg ggattagagc
     1501 ctttggaggt caaagcaaat taagattttt atttaagaat aaaagagtac tttgatcatg
     1561 agacataggt atcttgtccc tctcactaaa aaggagtgtt gtgtgtggcg gccacgtgct
     1621 tctatgtggt gtttgactct gtacaaattc tagtcccaaa gatcaagttg tctgaaggag
     1681 ccaaagtgtg aatgtgggtg tcggctgcgg cattaaattc atcatctcaa cccagagtgt
     1741 ctggtctccc tgctctttct gcatggttgt gtccctagtc ctaagctttg gttctttagg
     1801 gtgactgtgg taagaaggat atttaatcat gacatgcacg gacacgtaca tatttaactg
     1861 aaacaagttt taccaaacag tatttactcg tgatgtgcgt agtgcattct gatatttttg
     1921 agccattcta ttgtgttcta cttcacctaa aaaaataaaa taaaaatgtt gatcaaga
//
