LOCUS       NM_031899               3046 bp    mRNA    linear   PRI 02-JUN-2024
DEFINITION  Homo sapiens golgi reassembly stacking protein 1 (GORASP1),
            transcript variant 1, mRNA.
ACCESSION   NM_031899
VERSION     NM_031899.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Ahat,E., Song,Y., Xia,K., Reid,W., Li,J., Bui,S., Zhang,F.,
            Linhardt,R.J. and Wang,Y.
  TITLE     GRASP depletion-mediated Golgi fragmentation impairs
            glycosaminoglycan synthesis, sulfation, and secretion
  JOURNAL   Cell Mol Life Sci 79 (4), 199 (2022)
   PUBMED   35312866
  REMARK    GeneRIF: GRASP depletion-mediated Golgi fragmentation impairs
            glycosaminoglycan synthesis, sulfation, and secretion.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3046)
  AUTHORS   Zhang,Y. and Seemann,J.
  TITLE     Rapid degradation of GRASP55 and GRASP65 reveals their immediate
            impact on the Golgi structure
  JOURNAL   J Cell Biol 220 (1) (2021)
   PUBMED   33301566
  REMARK    GeneRIF: Rapid degradation of GRASP55 and GRASP65 reveals their
            immediate impact on the Golgi structure.
REFERENCE   4  (bases 1 to 3046)
  AUTHORS   Gordon,D.E., Hiatt,J., Bouhaddou,M., Rezelj,V.V., Ulferts,S.,
            Braberg,H., Jureka,A.S., Obernier,K., Guo,J.Z., Batra,J.,
            Kaake,R.M., Weckstein,A.R., Owens,T.W., Gupta,M., Pourmal,S.,
            Titus,E.W., Cakir,M., Soucheray,M., McGregor,M., Cakir,Z., Jang,G.,
            O'Meara,M.J., Tummino,T.A., Zhang,Z., Foussard,H., Rojc,A.,
            Zhou,Y., Kuchenov,D., Huttenhain,R., Xu,J., Eckhardt,M.,
            Swaney,D.L., Fabius,J.M., Ummadi,M., Tutuncuoglu,B., Rathore,U.,
            Modak,M., Haas,P., Haas,K.M., Naing,Z.Z.C., Pulido,E.H., Shi,Y.,
            Barrio-Hernandez,I., Memon,D., Petsalaki,E., Dunham,A.,
            Marrero,M.C., Burke,D., Koh,C., Vallet,T., Silvas,J.A.,
            Azumaya,C.M., Billesbolle,C., Brilot,A.F., Campbell,M.G.,
            Diallo,A., Dickinson,M.S., Diwanji,D., Herrera,N., Hoppe,N.,
            Kratochvil,H.T., Liu,Y., Merz,G.E., Moritz,M., Nguyen,H.C.,
            Nowotny,C., Puchades,C., Rizo,A.N., Schulze-Gahmen,U., Smith,A.M.,
            Sun,M., Young,I.D., Zhao,J., Asarnow,D., Biel,J., Bowen,A.,
            Braxton,J.R., Chen,J., Chio,C.M., Chio,U.S., Deshpande,I., Doan,L.,
            Faust,B., Flores,S., Jin,M., Kim,K., Lam,V.L., Li,F., Li,J.,
            Li,Y.L., Li,Y., Liu,X., Lo,M., Lopez,K.E., Melo,A.A., Moss,F.R.
            3rd, Nguyen,P., Paulino,J., Pawar,K.I., Peters,J.K., Pospiech,T.H.
            Jr., Safari,M., Sangwan,S., Schaefer,K., Thomas,P.V., Thwin,A.C.,
            Trenker,R., Tse,E., Tsui,T.K.M., Wang,F., Whitis,N., Yu,Z.,
            Zhang,K., Zhang,Y., Zhou,F., Saltzberg,D., Hodder,A.J.,
            Shun-Shion,A.S., Williams,D.M., White,K.M., Rosales,R., Kehrer,T.,
            Miorin,L., Moreno,E., Patel,A.H., Rihn,S., Khalid,M.M.,
            Vallejo-Gracia,A., Fozouni,P., Simoneau,C.R., Roth,T.L., Wu,D.,
            Karim,M.A., Ghoussaini,M., Dunham,I., Berardi,F., Weigang,S.,
            Chazal,M., Park,J., Logue,J., McGrath,M., Weston,S., Haupt,R.,
            Hastie,C.J., Elliott,M., Brown,F., Burness,K.A., Reid,E.,
            Dorward,M., Johnson,C., Wilkinson,S.G., Geyer,A., Giesel,D.M.,
            Baillie,C., Raggett,S., Leech,H., Toth,R., Goodman,N., Keough,K.C.,
            Lind,A.L., Klesh,R.J., Hemphill,K.R., Carlson-Stevermer,J., Oki,J.,
            Holden,K., Maures,T., Pollard,K.S., Sali,A., Agard,D.A., Cheng,Y.,
            Fraser,J.S., Frost,A., Jura,N., Kortemme,T., Manglik,A.,
            Southworth,D.R., Stroud,R.M., Alessi,D.R., Davies,P., Frieman,M.B.,
            Ideker,T., Abate,C., Jouvenet,N., Kochs,G., Shoichet,B., Ott,M.,
            Palmarini,M., Shokat,K.M., Garcia-Sastre,A., Rassen,J.A.,
            Grosse,R., Rosenberg,O.S., Verba,K.A., Basler,C.F., Vignuzzi,M.,
            Peden,A.A., Beltrao,P. and Krogan,N.J.
  CONSRTM   QCRG Structural Biology Consortium; Zoonomia Consortium
  TITLE     Comparative host-coronavirus protein interaction networks reveal
            pan-viral disease mechanisms
  JOURNAL   Science 370 (6521) (2020)
   PUBMED   33060197
REFERENCE   5  (bases 1 to 3046)
  AUTHORS   Reddy,S.T., Uversky,V.N. and Costa-Filho,A.J.
  TITLE     Biophysical characterization of intrinsically disordered human
            Golgi matrix protein GRASP65
  JOURNAL   Int J Biol Macromol 162, 1982-1993 (2020)
   PUBMED   32822731
  REMARK    GeneRIF: Biophysical characterization of intrinsically disordered
            human Golgi matrix protein GRASP65.
REFERENCE   6  (bases 1 to 3046)
  AUTHORS   Sutterlin,C., Lin,C.Y., Feng,Y., Ferris,D.K., Erikson,R.L. and
            Malhotra,V.
  TITLE     Polo-like kinase is required for the fragmentation of
            pericentriolar Golgi stacks during mitosis
  JOURNAL   Proc Natl Acad Sci U S A 98 (16), 9128-9132 (2001)
   PUBMED   11447294
REFERENCE   7  (bases 1 to 3046)
  AUTHORS   Moyer,B.D., Allan,B.B. and Balch,W.E.
  TITLE     Rab1 interaction with a GM130 effector complex regulates COPII
            vesicle cis--Golgi tethering
  JOURNAL   Traffic 2 (4), 268-276 (2001)
   PUBMED   11285137
REFERENCE   8  (bases 1 to 3046)
  AUTHORS   Lin,C.Y., Madsen,M.L., Yarm,F.R., Jang,Y.J., Liu,X. and
            Erikson,R.L.
  TITLE     Peripheral Golgi protein GRASP65 is a target of mitotic polo-like
            kinase (Plk) and Cdc2
  JOURNAL   Proc Natl Acad Sci U S A 97 (23), 12589-12594 (2000)
   PUBMED   11050165
REFERENCE   9  (bases 1 to 3046)
  AUTHORS   Barr,F.A., Nakamura,N. and Warren,G.
  TITLE     Mapping the interaction between GRASP65 and GM130, components of a
            protein complex involved in the stacking of Golgi cisternae
  JOURNAL   EMBO J 17 (12), 3258-3268 (1998)
   PUBMED   9628863
  REMARK    GeneRIF: A study showing that GRASP65 binds directly to the
            coiled-coil vesicle tethering factor GM130, and targets it to Golgi
            membranes.
REFERENCE   10 (bases 1 to 3046)
  AUTHORS   Barr,F.A., Puype,M., Vandekerckhove,J. and Warren,G.
  TITLE     GRASP65, a protein involved in the stacking of Golgi cisternae
  JOURNAL   Cell 91 (2), 253-262 (1997)
   PUBMED   9346242
  REMARK    GeneRIF: Identification of GRASP65, and demonstration that it
            functions in the formation of stacked Golgi cisternae.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK124755.1, AK057043.1,
            AK001574.1 and AI824080.1.
            
            On Aug 11, 2020 this sequence version replaced NM_031899.3.
            
            Summary: The Golgi complex plays a key role in the sorting and
            modification of proteins exported from the endoplasmic reticulum.
            The protein encoded by this gene is a membrane protein involved in
            establishing the stacked structure of the Golgi apparatus. It is a
            caspase-3 substrate, and cleavage of this encoded protein
            contributes to Golgi fragmentation in apoptosis. This encoded
            protein can form a complex with the Golgi matrix protein GOLGA2,
            and this complex binds to the vesicle docking protein p115.
            Alternative splicing results in multiple transcript variants of
            this gene. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK124755.1, AJ409349.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319283.8/ ENSP00000313869.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AK124755.1         737-762
            27-31               AK057043.1         38-42
            32-2107             AK124755.1         768-2843
            2108-2986           AK001574.1         2362-3240
            2987-3046           AI824080.1         8-67                c
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..3046
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /note="golgi reassembly stacking protein 1"
                     /db_xref="GeneID:64689"
                     /db_xref="HGNC:HGNC:16769"
                     /db_xref="MIM:606867"
     exon            1..149
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1409
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /note="isoform 1 is encoded by transcript variant 1; Golgi
                     reassembly and stacking protein 1; Golgi phosphoprotein 5;
                     Golgi peripheral membrane protein p65; golgi
                     reassembly-stacking protein of 65 kDa; golgi reassembly
                     stacking protein 1, 65kDa; Golgi reassembly-stacking
                     protein 1"
                     /codon_start=1
                     /product="Golgi reassembly-stacking protein 1 isoform 1"
                     /protein_id="NP_114105.1"
                     /db_xref="CCDS:CCDS2681.1"
                     /db_xref="GeneID:64689"
                     /db_xref="HGNC:HGNC:16769"
                     /db_xref="MIM:606867"
                     /translation="MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIG
                     HSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRF
                     CSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKP
                     LKLMVYNSKSDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPPSYHKKPPGTPP
                     PSALPLGAPPPDALPPGPTPEDSPSLETGSRQSDYMEALLQAPGSSMEDPLPGPGSPS
                     HSAPDPDGLPHFMETPLQPPPPVQRVMDPGFLDVSGISLLDNSNASVWPSLPSSTELT
                     TTAVSTSGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSL
                     TSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE"
     exon            150..230
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            231..434
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            435..521
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            522..652
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            653..851
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1002
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1155
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..3046
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2956..2961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /note="hexamer: AATAAA"
     polyA_site      2980
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /note="major polyA site"
     regulatory      3017..3022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
                     /note="hexamer: AATACA"
     polyA_site      3046
                     /gene="GORASP1"
                     /gene_synonym="GOLPH5; GRASP65; P65"
ORIGIN      
        1 gagagagcga gtcggtgcta ctggcgtcgg gtcggtccgg gtaggcgcag cgggactgga
       61 cctgggtgcc gagcggagcc gctgccatgg gcctgggcgt cagcgctgag cagcccgcag
      121 gcggcgccga gggcttccac ctccacgggg tgcaggagaa ctccccagcc cagcaggcgg
      181 gcctggagcc ctactttgac ttcatcatca ccattgggca ctcgaggctg aacaaggaga
      241 atgacaccct gaaggcacta ctgaaagcca atgtggagaa gcccgtgaag ctggaggtgt
      301 tcaatatgaa gaccatgagg gtgcgcgagg tggaggtggt gcccagcaac atgtggggcg
      361 gccagggcct actgggtgcc agtgtgcgct tctgcagctt ccgcagggcc agtgagcagg
      421 tgtggcatgt gctggatgtg gaaccatctt cacctgctgc ccttgccggc ctgcgcccct
      481 acacagacta tgtggttggt tcggaccaga ttctccagga gtccgaggac ttctttacgc
      541 tcatcgagtc tcatgagggg aagcccttga agctgatggt gtataactcc aagtcagact
      601 cctgccggga ggtgactgta actcccaacg cagcctgggg tggagagggc agtctgggat
      661 gtggcattgg ctatgggtat ctacaccgga tcccaactca gccccccagc taccacaaga
      721 agccacctgg caccccacca ccttctgctc taccacttgg tgccccacca cctgatgctc
      781 taccacctgg acccaccccc gaggactctc cttccctgga gacaggttcc aggcagagtg
      841 actacatgga ggccctgctg caggcacctg gctcctccat ggaggatccc cttcctgggc
      901 ctgggagtcc cagccacagt gctccagacc ctgatggact tccccatttc atggagactc
      961 ctcttcagcc cccacctcca gtgcagcgag ttatggaccc aggcttcctg gacgtgtcgg
     1021 gaatttctct cttggacaac agcaatgcca gtgtgtggcc cagcctgccc tcttccacag
     1081 aactgaccac cacagctgtc tcaacctcag ggccagagga catctgctcc agcagcagtt
     1141 ctcatgagcg gggtggtgag gctacatggt ctgggtcaga gtttgaggtc tccttcctgg
     1201 acagcccagg tgcccaagcc caggcggacc acctgcctca gctgactctt cctgacagtc
     1261 tcacctctgc agcctcacca gaagatgggc tgtccgccga gctgcttgaa gctcaggctg
     1321 aggaggaacc agcaagcaca gagggcctag atactgggac ggaggctgag gggctggaca
     1381 gccaggccca gatctctacc acagaataac accctgggct gtgacaaggc ccatgatgac
     1441 atttcatgag gcccagatgt gggcaggcag cccgggctgc actatgcggt caggattcct
     1501 tgctgctttg gtgggacatg aggcctcagt gggtggtgag agggggccat gggccttgtg
     1561 ggaggatatc ttttggccgt gtaccctcag gtccagtgct tggtcctgct ctggtcactg
     1621 gggcttccca ggaggcagca gggtctggtc ataatccagt gtgaagatga ggtgaggaag
     1681 gaaggggttg ctgtccagtg ttggcctggt cgctttgatt cttcctgcac agctcagccc
     1741 accccatctg tactggaagg gaccctggag ccctgggacc accccctttc aagtcccaaa
     1801 cactgagtcc tctgtcatgt gtccttgtcc ctaggctaca atgctacact tggctcaggc
     1861 tgatcaccat gctagtcaca ggagggagag agaggcagtg tacttgcccc catctcctgg
     1921 agggaggtgc gttggaaatt ctaggccctt cagcagagac ccatttgtgc ccagaccaca
     1981 atatttcttc ttcctcaggg actacatgcc acagacctgt atccccagtg gcaactcctg
     2041 ttagctccca aacttaaaca gtgatctctt ctaaatatac aaggcatcta cccagcccca
     2101 atagtgaata aaagtcacaa atttaagttc aacaccctaa gcaatttctc aaagccagtc
     2161 tacctgacta ggtctatcca cgcacggttc ccaaccccaa gggggaatta ggggattaga
     2221 ttaagagtag gtaaagaccc ttttgttctt tcctgttgta ggggaggaag ctgttctccc
     2281 caccccaggt gcctttggag gttaaggcta ggttaggccc tgcactccac ctggggacgg
     2341 cccctcatca cctgaagtcc tctgctgtgt tgactggacc ctcagttccc acactgctaa
     2401 ttactatgaa agctgctctg gcctcgtctt ctagcttgtg cctagttgaa agtctcacct
     2461 ttacaggatc atttgtaatg ctggtgtaca tgcagggaac tcacaagctg tgtcaggtat
     2521 gcaggacaag gaatgtctgc tctggggggc acaggccaac tatatggagg gagggtccca
     2581 aattagcaaa cccttcattt aaagcaaacc cttgtcaagc ctaccagagc agctgatacg
     2641 tgtgcataac agtgaggcat tgtgtcctgg atcgtctggg agcccagtgt caagtctgct
     2701 ggaatgcagg aagtagaaca gaatcgccac aggactgttc tggggccagc ttcccttaac
     2761 tctgtagcct ggcagtctga cccaaagttg ccctcaccca aaggttctgg ctcttccctc
     2821 cctcactttt actttccctt cccccataag ttggaggata aaatgggtat caatgctaat
     2881 atttccaggg agaacatgaa accagaggtt tctttctttc tctgtaatct gctatgaaag
     2941 aaaataacaa atgaaaataa atgtgtacta cactttgaaa tattttaact aaagccttta
     3001 ttctatacaa ctgtgaaata cagattttta cccttttggc attgca
//
