LOCUS       NM_001366282           11186 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens golgin B1 (GOLGB1), transcript variant 5, mRNA.
ACCESSION   NM_001366282 XM_006713587
VERSION     NM_001366282.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Casey,C.A., Thomes,P., Manca,S. and Petrosyan,A.
  TITLE     Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver
            Cells
  JOURNAL   Biomolecules 8 (4), 150 (2018)
   PUBMED   30453527
  REMARK    GeneRIF: Ethanol-induced Golgi disassembly was associated with
            de-dimerization of the largest Golgi matrix protein giantin, along
            with impaired transport of selected hepatic proteins.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Machado,R.A., Martelli-Junior,H., de Almeida Reis,S.R.,
            Persuhn,D.C. and Coletta,R.D.
  TITLE     Association between GOLGB1 tag-polymorphisms and nonsyndromic cleft
            palate only in the Brazilian population
  JOURNAL   Ann Hum Genet 82 (4), 227-231 (2018)
   PUBMED   29430628
  REMARK    GeneRIF: common polymorphisms of GOLGB1 are not associated with
            nonsyndromic cleft palate susceptibility in the Brazilian
            population
REFERENCE   3  (bases 1 to 11186)
  AUTHORS   Bhat,G., Hothpet,V.R., Lin,M.F. and Cheng,P.W.
  TITLE     Shifted Golgi targeting of glycosyltransferases and
            alpha-mannosidase IA from giantin to GM130-GRASP65 results in
            formation of high mannose N-glycans in aggressive prostate cancer
            cells
  JOURNAL   Biochim Biophys Acta Gen Subj 1861 (11 Pt A), 2891-2901 (2017)
   PUBMED   28782625
  REMARK    GeneRIF: In situ proximity ligation assays of Golgi localization of
            alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and
            absence or presence of N-glycans terminated with alpha3-mannose on
            trans-Golgi glycosyltransferases may be useful for distinguishing
            indolent from aggressive prostate cancer cells.
REFERENCE   4  (bases 1 to 11186)
  AUTHORS   Liu,W., Ge,S., Liu,Y., Wei,C., Ding,Y., Chen,A., Wu,Q. and Zhang,Y.
  TITLE     Polymorphisms in three genes are associated with hemorrhagic stroke
  JOURNAL   Brain Behav 5 (11), e00395 (2015)
   PUBMED   26664786
  REMARK    GeneRIF: Single nucleotide polymorphisms (SNPs) rs1035798 in RAGE
            gene, rs2073617 and rs2073618 in TNFRSF11B, and rs3732410 in Golgb1
            will be investigated on whether there is an association with
            hemorrhagic stroke (HS) in Chinese population.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11186)
  AUTHORS   Asante,D., Stevenson,N.L. and Stephens,D.J.
  TITLE     Subunit composition of the human cytoplasmic dynein-2 complex
  JOURNAL   J Cell Sci 127 (Pt 21), 4774-4787 (2014)
   PUBMED   25205765
REFERENCE   6  (bases 1 to 11186)
  AUTHORS   Linstedt,A.D., Jesch,S.A., Mehta,A., Lee,T.H., Garcia-Mata,R.,
            Nelson,D.S. and Sztul,E.
  TITLE     Binding relationships of membrane tethering components. The giantin
            N terminus and the GM130 N terminus compete for binding to the p115
            C terminus
  JOURNAL   J Biol Chem 275 (14), 10196-10201 (2000)
   PUBMED   10744704
REFERENCE   7  (bases 1 to 11186)
  AUTHORS   Sonnichsen,B., Lowe,M., Levine,T., Jamsa,E., Dirac-Svejstrup,B. and
            Warren,G.
  TITLE     A role for giantin in docking COPI vesicles to Golgi membranes
  JOURNAL   J Cell Biol 140 (5), 1013-1021 (1998)
   PUBMED   9490716
REFERENCE   8  (bases 1 to 11186)
  AUTHORS   Sohda,M., Misumi,Y., Fujiwara,T., Nishioka,M. and Ikehara,Y.
  TITLE     Molecular cloning and sequence analysis of a human 372-kDA protein
            localized in the Golgi complex
  JOURNAL   Biochem Biophys Res Commun 205 (2), 1399-1408 (1994)
   PUBMED   7802676
REFERENCE   9  (bases 1 to 11186)
  AUTHORS   Seelig,H.P., Schranz,P., Schroter,H., Wiemann,C. and Renz,M.
  TITLE     Macrogolgin--a new 376 kD Golgi complex outer membrane protein as
            target of antibodies in patients with rheumatic diseases and HIV
            infections
  JOURNAL   J Autoimmun 7 (1), 67-91 (1994)
   PUBMED   8198703
REFERENCE   10 (bases 1 to 11186)
  AUTHORS   Linstedt,A.D. and Hauri,H.P.
  TITLE     Giantin, a novel conserved Golgi membrane protein containing a
            cytoplasmic domain of at least 350 kDa
  JOURNAL   Mol Biol Cell 4 (7), 679-693 (1993)
   PUBMED   7691276
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC119736.7 and AC133750.2.
            
            On Jun 3, 2019 this sequence version replaced NM_001366282.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.2955302.1,
                                           SRR14038196.2769998.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000614479.5/ ENSP00000484083.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               AC119736.7         35656-35765         c
            111-208             AC119736.7         16900-16997         c
            209-361             AC119736.7         15889-16041         c
            362-514             AC119736.7         15212-15364         c
            515-643             AC119736.7         12937-13065         c
            644-760             AC119736.7         8286-8402           c
            761-883             AC119736.7         5670-5792           c
            884-997             AC119736.7         4412-4525           c
            998-1400            AC119736.7         2761-3163           c
            1401-1516           AC133750.2         86269-86384         c
            1517-1631           AC133750.2         73889-74003         c
            1632-1705           AC133750.2         71220-71293         c
            1706-6894           AC133750.2         65149-70337         c
            6895-8806           AC133750.2         62078-63989         c
            8807-8985           AC133750.2         53095-53273         c
            8986-9151           AC133750.2         48693-48858         c
            9152-9289           AC133750.2         48301-48438         c
            9290-9433           AC133750.2         40620-40763         c
            9434-9531           AC133750.2         39469-39566         c
            9532-9666           AC133750.2         38884-39018         c
            9667-9772           AC133750.2         36334-36439         c
            9773-11186          AC133750.2         34609-36022         c
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.33"
     gene            1..11186
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /note="golgin B1"
                     /db_xref="GeneID:2804"
                     /db_xref="HGNC:HGNC:4429"
                     /db_xref="MIM:602500"
     exon            1..110
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            111..208
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..9907
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /note="isoform 5 is encoded by transcript variant 5;
                     golgin B1, golgi integral membrane protein; golgi integral
                     membrane protein 1; golgi autoantigen, golgin subfamily b,
                     macrogolgin (with transmembrane signal), 1; golgin
                     subfamily B member 1; giantin; 372 kDa Golgi
                     complex-associated protein"
                     /codon_start=1
                     /product="golgin subfamily B member 1 isoform 5"
                     /protein_id="NP_001353211.1"
                     /db_xref="CCDS:CCDS93348.1"
                     /db_xref="GeneID:2804"
                     /db_xref="HGNC:HGNC:4429"
                     /db_xref="MIM:602500"
                     /translation="MLSRLSGLANVVLHELSGDDDTDQNMRAPLDPELHQESDMEFNN
                     TTQEDVQERLAYAEQLVVELKDIIRQKDVQLQQKDEALQEERKAADNKIKKLKLHAKA
                     KLTSLNKYIEEMKAQGGTVLPTEPQSEEQLSKHDKSSTEEEMEIEKIKHKLQEKEELI
                     STLQAQLTQAQAEQPAQSSTEMEEFVMMKQQLQEKEEFISTLQAQLSQTQAEQAAQLS
                     SMQQVVREKDARFETQVRLHEDELLQLVTQADVETEMQQKLRVLQRKLEEHEESLVGR
                     AQVVDLLQQELTAAEQRNQILSQQLQQMEAEHNTLRNTVETEREESKILLEKMELEVA
                     ERKLSFHNLQEEMHHLLEQFEQAGQAQAELESRYSALEQKHKAEMEEKTSHILSLQKT
                     GQELQSACDALKDQNSKLLQDKNEQAVQSAQTIQQLEDQLQQKSKEISQFLNRLPLQQ
                     HETASQTSFPDVYNEGTQAVTEENIASLQKRVVELENEKGALLLSSIELEELKAENEK
                     LSSQITLLEAQNRTGEADREVSEISIVDIANKRSSSAEESGQDVLENTFSQKHKELSV
                     LLLEMKEAQEEIAFLKLQLQGKRAEEADHEVLDQKEMKQMEGEGIAPIKMKVFLEDTG
                     QDFPLMPNEESSLPAVEKEQASTEHQSRTSEEISLNDAGVELKSTKQDGDKSLSAVPD
                     IGQCHQDELERLKSQILELELNFHKAQEIYEKNLDEKAKEISNLNQLIEEFKKNADNN
                     SSAFTALSEERDQLLSQVKELSMVTELRAQVKQLEMNLAEAERQRRLDYESQTAHDNL
                     LTEQIHSLSIEAKSKDVKIEVLQNELDDVQLQFSEQSTLIRSLQSQLQNKESEVLEGA
                     ERVRHISSKVEELSQALSQKELEITKMDQLLLEKKRDVETLQQTIEEKDQQVTEISFS
                     MTEKMVQLNEEKFSLGVEIKTLKEQLNLLSRAEEAKKEQVEEDNEVSSGLKQNYDEMS
                     PAGQISKEELQHEFDLLKKENEQRKRKLQAALINRKELLQRVSRLEEELANLKDESKK
                     EIPLSETERGEVEEDKENKEYSEKCVTSKCQEIEIYLKQTISEKEVELQHIRKDLEEK
                     LAAEEQFQALVKQMNQTLQDKTNQIDLLQAEISENQAIIQKLITSNTDASDGDSVALV
                     KETVVISPPCTGSSEHWKPELEEKILALEKEKEQLQKKLQEALTSRKAILKKAQEKER
                     HLREELKQQKDDYNRLQEQFDEQSKENENIGDQLRQLQIQVRESIDGKLPSTDQQESC
                     SSTPGLEEPLFKATEQHHTQPVLESNLCPDWPSHSEDASALQGGTSVAQIKAQLKEIE
                     AEKVELELKVSSTTSELTKKSEEVFQLQEQINKQGLEIESLKTVSHEAEVHAESLQQK
                     LESSQLQIAGLEHLRELQPKLDELQKLISKKEEDVSYLSGQLSEKEAALTKIQTEIIE
                     QEDLIKALHTQLEMQAKEHDERIKQLQVELCEMKQKPEEIGEESRAKQQIQRKLQAAL
                     ISRKEALKENKSLQEELSLARGTIERLTKSLADVESQVSAQNKEKDTVLGRLALLQEE
                     RDKLITEMDRSLLENQSLSSSCESLKLALEGLTEDKEKLVKEIESLKSSKIAESTEWQ
                     EKHKELQKEYEILLQSYENVSNEAERIQHVVEAVRQEKQELYGKLRSTEANKKETEKQ
                     LQEAEQEMEEMKEKMRKFAKSKQQKILELEEENDRLRAEVHPAGDTAKECMETLLSSN
                     ASMKEELERVKMEYETLSKKFQSLMSEKDSLSEEVQDLKHQIEGNVSKQANLEATEKH
                     DNQTNVTEEGTQSIPGETEEQDSLSMSTRPTCSESVPSAKSANPAVSKDFSSHDEINN
                     YLQQIDQLKERIAGLEEEKQKNKEFSQTLENEKNTLLSQISTKDGELKMLQEEVTKMN
                     LLNQQIQEELSRVTKLKETAEEEKDDLEERLMNQLAELNGSIGNYCQDVTDAQIKNEL
                     LESEMKNLKKCVSELEEEKQQLVKEKTKVESEIRKEYLEKIQGAQKEPGNKSHAKELQ
                     ELLKEKQQEVKQLQKDCIRYQEKISALERTVKALEFVQTESQKDLEITKENLAQAVEH
                     RKKAQAELASFKVLLDDTQSEAARVLADNLKLKKELQSNKESVKSQMKQKDEDLERRL
                     EQAEEKHLKEKKNMQEKLDALRREKVHLEETIGEIQVTLNKKDKEVQQLQENLDSTVT
                     QLAAFTKSMSSLQDDRDRVIDEAKKWERKFSDAIQSKEEEIRLKEDNCSVLKDQLRQM
                     SIHMEELKINISRLEHDKQIWESKAQTEVQLQQKVCDTLQGENKELLSQLEETRHLYH
                     SSQNELAKLESELKSLKDQLTDLSNSLEKCKEQKGNLEGIIRQQEADIQNSKFSYEQL
                     ETDLQASRELTSRLHEEINMKEQKIISLLSGKEEAIQVAIAELRQQHDKEIKELENLL
                     SQEEEENIVLEEENKKAVDKTNQLMETLKTIKKENIQQKAQLDSFVKSMSSLQNDRDR
                     IVGDYQQLEERHLSIILEKDQLIQEAAAENNKLKEEIRGLRSHMDDLNSENAKLDAEL
                     IQYREDLNQVITIKDSQQKQLLEVQLQQNKELENKYAKLEEKLKESEEANEDLRRSFN
                     ALQEEKQDLSKEIESLKVSISQLTRQVTALQEEGTLGLYHAQLKVKEEEVHRLSALFS
                     SSQKRIAELEEELVCVQKEAAKKVGEIEDKLKKELKHLHHDAGIMRNETETAEERVAE
                     LARDLVEMEQKLLMVTKENKGLTAQIQSFGRSMSSLQNSRDHANEELDELKRKYDASL
                     KELAQLKEQGLLNRERDALLSETAFSMNSTEENSLSHLEKLNQQLLSKDEQLLHLSSQ
                     LEDSYNQVQSFSKAMASLQNERDHLWNELEKFRKSEEGKQRSAAQPSTSPAEVQSLKK
                     AMSSLQNDRDRLLKELKNLQQQYLQINQEITELHPLKAQLQEYQDKTKAFQIMQEELR
                     QENLSWQHELHQLRMEKSSWEIHERRMKEQYLMAISDKDQQLSHLQNLIRELRSSSSQ
                     TQPLKVQYQRQASPETSASPDGSQNLVYETELLRTQLNDSLKEIHQKELRIQQLNSNF
                     SQLLEEKNTLSIQLCDTSQSLRENQQHYGDLLNHCAVLEKQVQELQAGPLNIDVAPGA
                     PQEKNGVHRKSDPEELREPQQSFSEAQQQLCNTRQEVNELRKLLEEERDQRVAAENAL
                     SVAEEQIRRLEHSEWDSSRTPIIGSCGTQEQALLIDLTSNSCRRTRSGVGWKRVLRSL
                     CHSRTRVPLLAAIYFLMIHVLLILCFTGHL"
     exon            209..361
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            362..514
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            515..643
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            644..760
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            761..883
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            884..997
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1400
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1516
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1631
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1705
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..6894
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            6895..8806
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            8807..8985
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            8986..9151
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            9152..9289
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            9290..9433
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            9434..9531
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            9532..9666
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            9667..9772
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            9773..11186
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10259..10264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /note="hexamer: AATAAA"
     polyA_site      10278
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /note="major polyA site"
     regulatory      11164..11169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /note="hexamer: AATAAA"
     polyA_site      11186
                     /gene="GOLGB1"
                     /gene_synonym="GCP; GCP372; GOLIM1"
                     /note="major polyA site"
ORIGIN      
        1 gagtccctgg cggggcgcgg cggtggaagg tgtcgcgtac gggcttcccg agctgacgtg
       61 gcttgaattg ggaggggggc agctggagcc tcaggcggca gcgcttctag aaatgctgag
      121 ccgattatca ggattagcaa atgttgtttt gcatgaatta tcaggagatg atgacactga
      181 tcagaatatg agggctcccc tagaccctga attacaccaa gaatctgaca tggaatttaa
      241 taatactaca caagaagatg ttcaggagcg cctggcttat gcagagcaat tggtggtgga
      301 gctaaaagat attattagac agaaggatgt tcaactgcag cagaaagatg aagctctaca
      361 ggaagagaga aaagctgctg ataacaaaat taaaaaacta aaacttcatg cgaaggccaa
      421 attaacttct ttgaataaat acatagaaga aatgaaagca caaggaggga ctgttctgcc
      481 tacagaacct cagtcagagg agcaactttc caagcatgac aagagttcta cagaggaaga
      541 gatggaaata gaaaagataa aacataagct ccaggagaag gaggaactaa tcagcacttt
      601 gcaagcccag cttactcagg cacaggcaga acaacctgca cagagttcta cagagatgga
      661 agaatttgta atgatgaagc aacagctcca ggagaaggaa gaattcatta gcactttaca
      721 agcccagctc agccagacac aggcagagca agctgcacag ttgagttcca tgcagcaggt
      781 ggtccgagag aaagatgccc gctttgaaac acaagttcgt cttcatgaag atgagcttct
      841 tcagttagta acccaggcag atgtggaaac agagatgcaa cagaaattga gggtgctgca
      901 aaggaagctt gaggaacacg aagaatcctt ggtgggccgt gctcaggtcg ttgacttgct
      961 gcaacaggag ctgactgctg ctgagcagag aaaccagatt ctctctcagc agttacagca
     1021 gatggaagct gagcataata ctttgaggaa cactgtggaa acagaaagag aggagtccaa
     1081 gattctactg gaaaagatgg aacttgaagt ggcagagaga aaattatcct tccataatct
     1141 gcaggaagaa atgcatcatc ttttagaaca gtttgagcaa gcaggccaag cccaggctga
     1201 actagagtct cggtatagtg ctttggagca gaagcacaaa gcagaaatgg aagagaagac
     1261 ctctcatatt ttgagtcttc aaaagactgg acaagagctg cagtctgcct gtgatgctct
     1321 aaaggatcaa aattcaaagc ttctccaaga taagaatgag caagcagttc agtcagccca
     1381 gaccattcag caactggaag atcagctcca gcaaaaatcc aaagaaatta gccaatttct
     1441 aaatagactg cccttgcaac aacatgaaac agcatctcag acttctttcc cagatgttta
     1501 taatgagggc acacaggcag tcactgagga gaatattgct tctttgcaga agagagtggt
     1561 agaactagag aatgaaaagg gagccttgct ccttagttct atagagctgg aggagctgaa
     1621 agctgagaat gaaaaactgt cttctcagat tactctccta gaggctcaga atagaactgg
     1681 ggaggcagac agagaagtca gtgagatcag cattgttgat attgccaaca agaggagctc
     1741 ttctgctgag gaaagtggac aagatgttct agaaaacaca ttttctcaga aacataaaga
     1801 attatcagtt ttattgttgg aaatgaaaga agctcaagag gaaattgcat ttcttaaatt
     1861 acagctccag ggaaaaaggg ctgaggaagc agatcatgag gtccttgacc agaaagaaat
     1921 gaaacagatg gagggtgagg gaatagctcc aattaaaatg aaagtatttc ttgaagatac
     1981 agggcaagat tttcccttaa tgccaaatga agagagcagt cttccagcag ttgaaaaaga
     2041 acaggcgagc actgaacatc aaagtagaac atctgaggaa atatctttaa atgatgctgg
     2101 agtagaattg aaatcaacaa agcaggatgg tgataaatcc ctttctgctg taccagatat
     2161 tggtcagtgt catcaggatg agttggaaag gttaaaaagt caaattttgg agctcgagct
     2221 aaactttcat aaagcacaag aaatctatga gaaaaattta gatgagaaag ctaaggaaat
     2281 tagcaaccta aaccagttga ttgaggagtt taagaaaaat gctgacaaca acagcagtgc
     2341 attcactgct ttgtctgaag aaagagacca gcttctctct caggtgaagg aacttagcat
     2401 ggtaacagaa ttgagggctc aggtaaagca actggaaatg aaccttgcag aagcagaaag
     2461 gcaaagaaga cttgattatg aaagccaaac tgcccatgac aacctgctca ctgaacagat
     2521 ccatagtctc agcatagaag ccaaatctaa agatgtgaaa attgaagttt tacagaatga
     2581 actggatgat gtgcagcttc agttttctga gcagagtacc ctgataagaa gcctgcaaag
     2641 ccagctgcaa aataaggaaa gtgaagtgct tgagggggca gaacgtgtaa ggcatatctc
     2701 aagtaaagtg gaagaactgt cccaggctct ttcacagaag gaacttgaaa taacaaaaat
     2761 ggatcagctc ttactagaga aaaagagaga tgtggaaacc ctccaacaaa ccatcgagga
     2821 gaaggatcaa caagtgacag aaatcagctt tagtatgact gagaaaatgg ttcagcttaa
     2881 tgaagagaag ttttctcttg gggttgaaat taagactctt aaagaacagc taaatttatt
     2941 atccagagct gaggaagcaa aaaaagagca ggtggaagaa gataatgaag tttcttctgg
     3001 ccttaaacaa aattatgatg agatgagccc agcaggacaa ataagtaagg aagaacttca
     3061 gcatgaattt gaccttctga agaaagaaaa tgagcagaga aagagaaagc tccaggcagc
     3121 tcttattaac agaaaggagc ttctgcaaag agtcagtaga ttggaagaag aattagccaa
     3181 cttgaaagat gaatctaaga aagaaatccc actcagtgag actgagaggg gagaagtgga
     3241 agaagataaa gaaaacaaag aatactcaga aaaatgtgtg acttctaagt gccaagaaat
     3301 agaaatttat ttaaaacaga caatatctga gaaagaagtg gaactacagc atataaggaa
     3361 ggatttggaa gaaaagctgg cagctgaaga gcaattccag gctctggtca aacagatgaa
     3421 tcagaccttg caagataaaa caaaccaaat agatttgctc caagcagaaa tcagtgaaaa
     3481 ccaagcaatt atccagaagt taatcacaag taacacggat gcaagtgatg gggactccgt
     3541 agcacttgta aaggaaacag tggtgataag tccaccttgt acaggtagta gtgaacactg
     3601 gaaaccagaa ctagaagaaa agatactggc ccttgaaaaa gaaaaggagc aacttcaaaa
     3661 gaagctacag gaagccttaa cctcccgcaa ggcaattctt aaaaaggcac aggagaaaga
     3721 aagacatctc agggaggagc taaagcaaca gaaagatgac tataatcgct tgcaagaaca
     3781 gtttgatgag caaagcaagg aaaatgagaa tattggagac cagctaaggc aactccagat
     3841 tcaagtaagg gaatccatag acggaaaact cccaagcaca gaccagcagg aatcgtgttc
     3901 ttccactcca ggtttagaag aacctttatt caaagccaca gaacagcatc acactcaacc
     3961 tgttttagag tccaacttgt gcccagactg gccttctcat tctgaagatg cgagtgctct
     4021 gcagggcgga acttctgttg cccagattaa ggcccagctg aaggaaatag aggctgagaa
     4081 agtagagtta gaattgaaag ttagttctac aacaagtgag cttactaaaa aatcagaaga
     4141 ggtatttcag ttacaagagc agataaataa acagggttta gaaatcgaga gtctaaagac
     4201 agtatcccat gaagctgaag tccatgccga aagcctgcag cagaaattgg aaagcagcca
     4261 actacaaatt gctggcctag aacatctaag agaattgcaa cctaaactgg atgaactgca
     4321 aaaactcata agcaaaaagg aagaagacgt tagctacctt tctggacaac ttagtgagaa
     4381 agaagcagct ctcactaaaa tacagacaga gataatagaa caagaagatt taattaaggc
     4441 tctgcataca cagctagaaa tgcaagccaa agagcatgat gagaggataa agcagctaca
     4501 ggtggaactt tgtgaaatga agcaaaaacc agaagagatt ggagaagaaa gtagagcaaa
     4561 gcaacaaata caaaggaaac tgcaagctgc ccttatttcc cgaaaagaag cactaaaaga
     4621 aaacaaaagt ctccaagagg aattgtcttt ggccagaggt accattgaac gtctcaccaa
     4681 gtctctggca gatgtggaaa gccaagtttc tgctcaaaat aaagaaaaag atacggtctt
     4741 aggaaggtta gctcttcttc aagaagaaag agacaaactc attacagaaa tggacaggtc
     4801 tttattggaa aatcagagtc tcagcagctc ctgtgaaagt ctaaaactag ctctagaggg
     4861 tcttactgaa gacaaggaaa agttagtgaa ggaaattgaa tctttgaaat cttctaagat
     4921 tgcagaaagt actgagtggc aagagaaaca caaggagcta caaaaagagt atgaaattct
     4981 tctgcagtcc tatgagaatg ttagtaatga agcagaaagg attcagcatg tggtggaagc
     5041 tgtgaggcaa gagaaacaag aactgtatgg caagttaaga agcacagagg caaacaagaa
     5101 ggagacagaa aagcagttgc aggaagctga gcaagaaatg gaggaaatga aagaaaagat
     5161 gagaaagttt gctaaatcta aacagcagaa aatcctagag ctggaagaag agaatgaccg
     5221 gcttagggca gaggtgcacc ctgcaggaga tacagctaaa gagtgtatgg aaacacttct
     5281 ttcttccaat gccagcatga aggaagaact tgaaagggtc aaaatggagt atgaaaccct
     5341 ttctaagaag tttcagtctt taatgtctga gaaagactct ctaagtgaag aggttcaaga
     5401 tttaaagcat cagatagaag gtaatgtatc taaacaagct aacctagagg ccaccgagaa
     5461 acatgataac caaacgaatg tcactgaaga gggaacacag tctataccag gtgagactga
     5521 agagcaagac tctctgagta tgagcacaag acctacatgt tcagaatcgg ttccatcagc
     5581 gaagagtgcc aaccctgctg taagtaagga tttcagctca catgatgaaa ttaataacta
     5641 cctacagcag attgatcagc tcaaagaaag aattgctgga ttagaggagg agaagcagaa
     5701 aaacaaggaa tttagccaga ctttagaaaa tgagaaaaat accttactga gtcagatatc
     5761 aacaaaggat ggtgaactaa aaatgcttca ggaggaagta accaaaatga acctgttaaa
     5821 tcagcaaatc caagaagaac tctccagagt taccaaacta aaggagacag cagaagaaga
     5881 gaaagatgat ttggaagaga ggcttatgaa tcaattagca gaacttaatg gaagcattgg
     5941 gaattactgt caggatgtta cagatgccca aataaaaaat gagctattgg aatctgaaat
     6001 gaagaacctt aaaaagtgtg tgagtgaatt ggaagaagaa aagcagcagt tagtcaagga
     6061 aaaaactaag gtggaatcag aaatacgaaa ggaatatttg gagaaaatac aaggtgctca
     6121 gaaagaaccc ggaaataaaa gccatgcaaa ggaacttcag gaactgttaa aagaaaaaca
     6181 acaagaagta aagcagctac agaaggactg catcaggtat caagagaaaa ttagtgctct
     6241 ggagagaact gttaaagctc tagaatttgt tcaaactgaa tctcaaaaag atttggaaat
     6301 aaccaaagaa aatctggctc aagcagttga acaccgcaaa aaggcacaag cagaattagc
     6361 tagcttcaaa gtcctgctag atgacactca aagtgaagca gcaagggtcc tagcagacaa
     6421 tctcaagttg aaaaaggaac ttcagtcaaa taaagaatca gttaaaagcc agatgaaaca
     6481 aaaggatgaa gatcttgagc gaagactgga acaggcagaa gagaagcacc tgaaagagaa
     6541 gaagaatatg caagagaaac tggatgcttt gcgcagagaa aaagtccact tggaagagac
     6601 aattggagag attcaggtta ctttgaacaa gaaagacaag gaagttcagc aacttcagga
     6661 aaacttggac agtactgtga cccagcttgc agcctttact aagagcatgt cttccctcca
     6721 ggatgatcgt gacagggtga tagatgaagc taagaaatgg gagaggaagt ttagtgatgc
     6781 gattcaaagc aaagaagaag aaattagact caaagaagat aattgcagtg ttctaaagga
     6841 tcaacttaga cagatgtcca tccatatgga agaattaaag attaacattt ccaggcttga
     6901 acatgacaag cagatttggg agtccaaggc ccagacagag gtccagcttc agcagaaggt
     6961 ctgtgatact ctacaggggg aaaacaaaga acttttgtcc cagctagaag agacacgcca
     7021 cctataccac agttctcaga atgaattagc taagttggaa tcagaactta agagtctcaa
     7081 agaccagttg actgatttaa gtaactcttt agaaaaatgt aaggaacaaa aaggaaactt
     7141 ggaagggatc ataaggcagc aagaggctga tattcaaaat tctaagttca gttatgaaca
     7201 actggagact gatcttcagg cctccagaga actgaccagt aggctgcatg aagaaataaa
     7261 tatgaaagag caaaagatta taagcctgct ttctggcaag gaagaggcaa tccaagtagc
     7321 tattgctgaa ctgcgtcagc aacatgataa agaaattaaa gagctggaaa acctgctgtc
     7381 ccaggaggaa gaggagaata ttgttttaga agaggagaac aaaaaggctg ttgataaaac
     7441 caatcagctt atggaaacac tgaaaaccat caaaaaggaa aacattcagc aaaaggcaca
     7501 gttggattcc tttgttaaat ccatgtcttc tctccaaaat gatcgagacc gcatagtggg
     7561 tgactatcaa cagctggaag agcgacatct ctctataatc ttggaaaaag accaactcat
     7621 ccaagaggct gctgcagaga ataataagct taaagaagaa atacgaggct tgagaagtca
     7681 tatggatgat ctcaattctg agaatgccaa gctagatgca gaactgatcc aatatagaga
     7741 agacctgaac caagtgataa caataaagga cagccaacaa aagcagcttc ttgaagttca
     7801 acttcagcaa aataaggagc tggaaaataa atatgctaaa ttagaagaaa agctgaagga
     7861 atctgaggaa gcaaatgagg atctgcggag gtcctttaat gccctacaag aagagaaaca
     7921 agatttatct aaagagattg agagtttgaa agtatctata tcccagctaa caagacaagt
     7981 aacagccttg caagaagaag gtactttagg actctatcat gcccagttaa aagtaaaaga
     8041 agaagaggta cacaggttaa gtgctttgtt ttcctcctct caaaagagaa ttgcagaact
     8101 ggaagaagaa ttggtttgtg ttcaaaagga agctgccaag aaggtaggtg aaattgaaga
     8161 taaactgaag aaagaattaa agcatcttca tcatgatgca gggataatga gaaatgaaac
     8221 tgaaacagca gaagagagag tggcagagct agcaagagat ttggtggaga tggaacagaa
     8281 attactcatg gtcaccaaag aaaataaagg tctcacagca caaattcagt cttttggaag
     8341 gtctatgagt tccttgcaaa atagtagaga tcatgccaat gaggaacttg atgaactgaa
     8401 aaggaaatat gatgccagtc tgaaggaatt ggcacagttg aaagaacagg gactcttaaa
     8461 cagagagaga gatgctcttc tttctgaaac cgccttttca atgaactcca ctgaggagaa
     8521 tagcttgtct caccttgaga aacttaacca acagctccta tccaaagatg agcaattgct
     8581 tcacttgtcc tcacaactag aagattctta taaccaagtg cagtcctttt ccaaggctat
     8641 ggccagtctg cagaatgaga gagatcacct gtggaatgag ctggagaaat ttcgaaagtc
     8701 agaggaaggg aagcagaggt ctgcagctca gccttccacc agcccagctg aagtacagag
     8761 tttaaaaaaa gctatgtctt cactccaaaa tgacagagac agactactga aggaattgaa
     8821 gaatctgcag cagcaatact tacagattaa tcaagagatc actgagttac atccactgaa
     8881 ggctcaactt caggagtatc aagataagac aaaagcattt cagattatgc aagaagagct
     8941 caggcaggaa aacctctcct ggcagcatga gctgcatcag ctcaggatgg agaagagttc
     9001 ctgggaaata catgagagga gaatgaagga acagtacctt atggctatct cagataaaga
     9061 tcagcagctc agtcatctgc agaatcttat aagggaattg aggtcttctt cctcccagac
     9121 tcagcctctc aaagtgcaat accaaagaca ggcatcccca gagacatcag cttccccaga
     9181 tgggtcacaa aatctggttt atgagacaga acttctcagg acccagctca atgacagctt
     9241 aaaggaaatt caccaaaagg agttaagaat tcagcaactg aacagcaact tctctcagct
     9301 actggaagag aaaaacaccc tttccattca gctctgcgat accagtcaga gtcttcgtga
     9361 gaaccagcag cactatggtg accttttaaa tcactgtgca gtcttggaga agcaggttca
     9421 agagctgcag gcggggccac taaatataga tgttgctcca ggagctcccc aggaaaagaa
     9481 tggagttcac agaaagagtg accctgagga actaagggaa ccgcagcaaa gcttttctga
     9541 agctcagcag cagctatgca acaccagaca ggaagtgaat gaattaagga agctgctgga
     9601 agaagaacga gaccaaagag tggctgctga gaatgctctc tctgtggccg aggagcagat
     9661 cagacggtta gagcacagtg aatgggactc ttcccggact cctatcattg gctcctgtgg
     9721 cactcaggag caggcactgt taatagatct tacaagcaac agttgtcgaa ggacccggag
     9781 tggcgttgga tggaagcgag tcctgcgttc actctgtcat tcacggaccc gagtgccact
     9841 tctagcagcc atctactttc taatgattca tgtcctgctc attctgtgtt ttacgggcca
     9901 tctatagact tagttgttac tctttggacc actcccctca aaacttggaa ttctctcacc
     9961 tctaacatca gaacatcaat tccagtggaa cagtcttccc atttacaggt cttctctcca
    10021 actcttcacg gaaagtgcct gcaaaaacag aggtggatac gaggacaggt tggagctgca
    10081 gggactggcg agtctgcttt cttctactgc cctgagcctg aacgcttctg cttaatctga
    10141 gaatcacatt tggtttgttg agcctaatat ttgttgagat tttgcaggac cctgatcttt
    10201 tgtggtcctg taaaagatac tgaggaatgt ctttcagcca agccaagagg atggtttcaa
    10261 taaacctaat aatctgaagt tcagtatcat tttgattgat aactttcttt gccttgtttg
    10321 cctgtatttt ctcctgtttc agggggaagg tggctctgcc ataaacagag ttcagggaaa
    10381 tgaacatgtc acctcatagg acatctgatt taggtctctt gcagaatagg gtggtgaaag
    10441 ctgaaggaac tccctagggg ttttagctgt tagatcacat gggaggacag catcttcttc
    10501 cctgccaatt tcagatataa ttggagggag aatttcaggt cttagactat aaggatagaa
    10561 tccctttgct tttgcccttg atcacatgta tactaaggta ttaagtggcc caagtctttc
    10621 ctttcaccaa agggcaggga gaagtgtcta tggacagcag ggttccaatt cttgtcattc
    10681 caggaatatc tgaatattcc tggaatgtgg accctgagac agtgctcagg ggccactggg
    10741 agccaatatt aggcattgac tctcagccag gagtctgact ggtctaacac cacactgcaa
    10801 ctcctgacct cttgaagtac taagctactt tgctgttagt gacagttatt acagtttctc
    10861 agccccattg tctctgccct ctgtggcaat ggaaggagaa tatagagaag acaaaattaa
    10921 atatagatag acctgagaag gacagccagg atagaattcc atttgtgcct attccctgcc
    10981 tcccctcccc tcccccatcc taccagttgg ttattttctc attgcatacg tgatgtgttc
    11041 actgcctagc ctctccctaa agaagagaga agacaagtgg gctgactgat ctgctctaaa
    11101 tctactgtgg tgattaaatc ttggttacaa catcctggga agtttcctga acaactgtaa
    11161 aataataaaa ttattttcag aatgaa
//
