LOCUS       NM_001410721            7809 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens golgin A4 (GOLGA4), transcript variant 3, mRNA.
ACCESSION   NM_001410721 XM_047447982
VERSION     NM_001410721.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Downes,C.E.J., Rehn,J., Heatley,S.L., Yeung,D., McClure,B.J. and
            White,D.L.
  TITLE     Identification of a novel GOLGA4-JAK2 fusion gene in B-cell acute
            lymphoblastic leukaemia
  JOURNAL   Br J Haematol 196 (3), 700-705 (2022)
   PUBMED   34697799
  REMARK    GeneRIF: Identification of a novel GOLGA4-JAK2 fusion gene in
            B-cell acute lymphoblastic leukaemia.
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Cao,Y., Lei,Y., Luo,Y., Tan,T., Du,B., Zheng,Y., Sun,L. and
            Liang,Q.
  TITLE     The actomyosin network is influenced by NMHC IIA and regulated by
            CrpF46, which is involved in controlling cell migration
  JOURNAL   Exp Cell Res 373 (1-2), 119-131 (2018)
   PUBMED   30336116
  REMARK    GeneRIF: CrpF46 regulates cell migration by centrosomal
            reorientation and altering the function of the actomyosin network
            by controlling specific phosphorylation of myosin.
REFERENCE   3  (bases 1 to 7809)
  AUTHORS   Kork,F., Jankowski,J., Goswami,A., Weis,J., Brook,G., Yamoah,A.,
            Anink,J., Aronica,E., Fritz,S., Huck,C., Schipke,C., Peters,O.,
            Tepel,M., Noels,H. and Jankowski,V.
  TITLE     Golgin A4 in CSF and granulovacuolar degenerations of patients with
            Alzheimer disease
  JOURNAL   Neurology 91 (19), e1799-e1808 (2018)
   PUBMED   30305446
  REMARK    GeneRIF: This study provides Class III evidence that elevated
            cerebrospinal fluid golgin A4 levels identify patients with
            Alzheimer disease.
REFERENCE   4  (bases 1 to 7809)
  AUTHORS   Shin,J.J.H., Gillingham,A.K., Begum,F., Chadwick,J. and Munro,S.
  TITLE     TBC1D23 is a bridging factor for endosomal vesicle capture by
            golgins at the trans-Golgi
  JOURNAL   Nat Cell Biol 19 (12), 1424-1432 (2017)
   PUBMED   29084197
  REMARK    Erratum:[Nat Cell Biol. 2018 Jan 8;:. PMID: 29311657]
REFERENCE   5  (bases 1 to 7809)
  AUTHORS   Sohda,M., Misumi,Y., Ogata,S., Sakisaka,S., Hirose,S., Ikehara,Y.
            and Oda,K.
  TITLE     Trans-Golgi protein p230/golgin-245 is involved in phagophore
            formation
  JOURNAL   Biochem Biophys Res Commun 456 (1), 275-281 (2015)
   PUBMED   25436429
  REMARK    GeneRIF: p230 is an important molecule in phagophore formation,
            although it remains unclear whether p230 has any role in late steps
            of autophagy
REFERENCE   6  (bases 1 to 7809)
  AUTHORS   Barr,F.A.
  TITLE     A novel Rab6-interacting domain defines a family of Golgi-targeted
            coiled-coil proteins
  JOURNAL   Curr Biol 9 (7), 381-384 (1999)
   PUBMED   10209123
REFERENCE   7  (bases 1 to 7809)
  AUTHORS   Gleeson,P.A., Anderson,T.J., Stow,J.L., Griffiths,G., Toh,B.H. and
            Matheson,F.
  TITLE     p230 is associated with vesicles budding from the trans-Golgi
            network
  JOURNAL   J Cell Sci 109 (Pt 12), 2811-2821 (1996)
   PUBMED   9013329
REFERENCE   8  (bases 1 to 7809)
  AUTHORS   Erlich,R., Gleeson,P.A., Campbell,P., Dietzsch,E. and Toh,B.H.
  TITLE     Molecular characterization of trans-Golgi p230. A human peripheral
            membrane protein encoded by a gene on chromosome 6p12-22 contains
            extensive coiled-coil alpha-helical domains and a granin motif
  JOURNAL   J Biol Chem 271 (14), 8328-8337 (1996)
   PUBMED   8626529
REFERENCE   9  (bases 1 to 7809)
  AUTHORS   Fritzler,M.J., Lung,C.C., Hamel,J.C., Griffith,K.J. and Chan,E.K.
  TITLE     Molecular characterization of Golgin-245, a novel Golgi complex
            protein containing a granin signature
  JOURNAL   J Biol Chem 270 (52), 31262-31268 (1995)
   PUBMED   8537393
REFERENCE   10 (bases 1 to 7809)
  AUTHORS   Kooy,J., Toh,B.H., Pettitt,J.M., Erlich,R. and Gleeson,P.A.
  TITLE     Human autoantibodies as reagents to conserved Golgi components.
            Characterization of a peripheral, 230-kDa compartment-specific
            Golgi protein
  JOURNAL   J Biol Chem 267 (28), 20255-20263 (1992)
   PUBMED   1400343
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC097359.2.
            
            [WARNING] On Apr 12, 2024 this sequence was replaced by
            NM_001410721.2.
            
            On Aug 15, 2022 this sequence version replaced XM_047447982.1.
            
            Summary: The Golgi apparatus, which participates in glycosylation
            and transport of proteins and lipids in the secretory pathway,
            consists of a series of stacked cisternae (flattened membrane
            sacs). Interactions between the Golgi and microtubules are thought
            to be important for the reorganization of the Golgi after it
            fragments during mitosis. This gene encodes one of the golgins, a
            family of proteins localized to the Golgi. This protein has been
            postulated to play a role in Rab6-regulated membrane-tethering
            events in the Golgi apparatus. Alternatively spliced transcript
            variants encoding different isoforms have been identified in this
            gene. [provided by RefSeq, Feb 2010].
            
            Transcript Variant: This variant (3) encodes the longest isoform
            (3).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2179616.1,
                                           SRR14038195.1115696.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-352               AC097359.2         62304-62655
            353-442             AC097359.2         70428-70517
            443-508             AC097359.2         92569-92634
            509-823             AC097359.2         100991-101305
            824-871             AC097359.2         105047-105094
            872-928             AC097359.2         108268-108324
            929-1027            AC097359.2         114012-114110
            1028-1160           AC097359.2         115120-115252
            1161-1348           AC097359.2         117866-118053
            1349-1432           AC097359.2         118321-118404
            1433-1580           AC097359.2         121218-121365
            1581-1759           AC097359.2         134453-134631
            1760-1891           AC097359.2         138096-138227
            1892-2047           AC097359.2         140764-140919
            2048-6285           AC097359.2         142621-146858
            6286-6407           AC097359.2         147449-147570
            6408-6538           AC097359.2         147996-148126
            6539-6652           AC097359.2         154086-154199
            6653-6673           AC097359.2         156176-156196
            6674-6742           AC097359.2         156699-156767
            6743-6818           AC097359.2         159157-159232
            6819-6922           AC097359.2         166226-166329
            6923-7009           AC097359.2         174134-174220
            7010-7809           AC097359.2         185113-185912
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..7809
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /note="golgin A4"
                     /db_xref="GeneID:2803"
                     /db_xref="HGNC:HGNC:4427"
                     /db_xref="MIM:602509"
     exon            1..352
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..7033
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /note="isoform 3 is encoded by transcript variant 3; golgi
                     autoantigen, golgin subfamily a, 4; 256 kDa golgin; 72.1
                     protein; golgin-245; trans-Golgi p230; golgin subfamily A
                     member 4; golgin-240; protein 72.1; centrosome-related
                     protein F46"
                     /codon_start=1
                     /product="golgin subfamily A member 4 isoform 3"
                     /protein_id="NP_001397650.1"
                     /db_xref="CCDS:CCDS93242.1"
                     /db_xref="GeneID:2803"
                     /db_xref="HGNC:HGNC:4427"
                     /db_xref="MIM:602509"
                     /translation="MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTE
                     QLDEGTPNRENASTHASKSPDSVNGSEPSIPQSGDTQSFAQKLQLRVPSVESLFRSPI
                     KESLFRSSSKESLVRTSSRESLNRLDLDSSTASFDPPSDMDSEAEDLVGNSDSLNKEQ
                     LIQRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQGILSQSQDKSLRRIAELREEL
                     QMDQQAKKHLQEEFDASLEEKDQYISVLQTQVSLLKQRLRNGPMNVDVLKPLPQLEPQ
                     AEVFTKEENPESDGEPVVEDGTSVKTLETLQQRVKRQENLLKRCKETIQSHKEQCTLL
                     TSEKEALQEQLDERLQELEKIKDLHMAEKTKLITQLRDAKNLIEQLEQDKGMVIAETK
                     RQMHETLEMKEEEIAQLRSRIKQMTTQGEELREQKEKSERAAFEELEKALSTAQKTEE
                     ARRKLKAEMDEQIKTIEKTSEEERISLQQELSRVKQEVVDVMKKSSEEQIAKLQKLHE
                     KELARKEQELTKKLQTREREFQEQMKVALEKSQSEYLKISQEKEQQESLALEELELQK
                     KAILTESENKLRDLQQEAETYRTRILELESSLEKSLQENKNQSKDLAVHLEAEKNKHN
                     KEITVMVEKHKTELESLKHQQDALWTEKLQVLKQQYQTEMEKLREKCEQEKETLLKDK
                     EIIFQAHIEEMNEKTLEKLDVKQTELESLSSELSEVLKARHKLEEELSVLKDQTDKMK
                     QELEAKMDEQKNHHQQQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKHLKEHQ
                     AHVENLEADIKRSEGELQQASAKLDVFQSYQSATHEQTKAYEEQLAQLQQKLLDLETE
                     RILLTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKSLTQVYESKL
                     EDGNKEQEQTKQILVEKENMILQMREGQKKEIEILTQKLSAKEDSIHILNEEYETKFK
                     NQEKKMEKVKQKAKEMQETLKKKLLDQEAKLKKELENTALELSQKEKQFNAKMLEMAQ
                     ANSAGISDAVSRLETNQKEQIESLTEVHRRELNDVISIWEKKLNQQAEELQEIHEIQL
                     QEKEQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHVNS
                     LAQDETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSELTSKLKTTD
                     EEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDICCKKTEALLEAKTNELINISS
                     SKTNAILSRISHCQHRTTKVKEALLIKTCTVSELEAQLRQLTEEQNTLNISFQQATHQ
                     LEEKENQIKSMKADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENINAVT
                     LMKEELKEKKVEISSLSKQLTDLNVQLQNSISLSEKEAAISSLRKQYDEEKCELLDQV
                     QDLSFKVDTLSKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSK
                     EAYEKDEQINLLKEELDQQNKRFDCLKGEMEDDKSKMEKKESNLETELKSQTARIMEL
                     EDHITQKTIEIESLNEVLKNYNQQKDIEHKELVQKLQHFQELGEEKDNRVKEAEEKIL
                     TLENQVYSMKAELETKKKELEHVNLSVKSKEEELKALEDRLESESAAKLAELKRKAEQ
                     KIAAIKKQLLSQMEEKEEQYKKGTESHLSELNTKLQEREREVHILEEKLKSVESSQSE
                     TLIVPRSAKNVAAYTEQEEADSQGCVQKTYEEKISVLQRNLTEKEKLLQRVGQEKEET
                     VSSHFEMRCQYQERLIKLEHAEAKQHEDQSMIGHLQEELEEKNKKYSLIVAQHVEKEG
                     GKNNIQAKQNLENVFDDVQKTLQEKELTCQILEQKIKELDSCLVRQKEVHRVEMEELT
                     SKYEKLQALQQMDGRNKPTELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAGAE
                     REKQKLGKEIVRLQKDLRMLRKEHQQELEILKKEYDQEREEKIKQEQEDLELKHNSTL
                     KQLMREFNTQLAQKEQELEMTIKETINKAQEVEAELLESHQEETNQLLKKIAEKDDDL
                     KRTAKRYEEILDAREEEMTAKVRDLQTQLEELQKKYQQKLEQEENPGNDNVTIMELQT
                     QLAQKTTLISDSKLKEQEFREQIHNLEDRLKKYEKNVYATTVGTPYKGGNLYHTDVSL
                     FGEPTEFEYLRKVLFEYMMGRETKTMAKVITTVLKFPDDQTQKILEREDARLMSWLRS
                     SS"
     exon            353..442
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            443..508
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            509..823
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            824..871
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            872..928
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1027
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1160
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1348
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1432
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1580
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1759
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1891
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2047
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..6285
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6286..6407
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6408..6538
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6539..6652
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6653..6673
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6674..6742
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6743..6818
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6819..6922
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            6923..7009
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     exon            7010..7809
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7657..7662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /note="hexamer: AATAAA"
     polyA_site      7681
                     /gene="GOLGA4"
                     /gene_synonym="CRPF46; GCP2; GOLG; MU-RMS-40.18; p230"
                     /note="major polyA site"
ORIGIN      
        1 agttcacctg ctgccgttgt cgtcgccgcc gcggctcccg gggctggatg gggggccgag
       61 gccagccagt ggcacccgga agaaagagac gcggcggcgg cgacgccgac accctcagga
      121 cgagtgtccg gacttgccca cagcctcaag gaggagacgg cgaggcccgg cccccgctgt
      181 ccctggtgta aagaagtcgc cgtagccgtc gcggccggga ctccccgggc tctcgccctt
      241 caggtttcgt tgacactcag gaccgtacgt acgctgcgcc atgttcaaga aactgaagca
      301 aaagatcagc gaggagcagc agcagctcca gcaggcgctg gctcctgctc aggcgtcctc
      361 caattcttca acaccaacaa gaatgaggag caggacatct tcatttacag agcaacttga
      421 tgaaggtaca cccaatagag agaatgcatc tactcatgcc tcgaaatctc ctgacagtgt
      481 taatggaagt gaaccaagca ttcctcagtc aggtgacaca cagtcttttg cacagaagct
      541 ccagctccgg gtgccctccg tggagtcttt gtttcgaagt ccgataaagg aatctctatt
      601 ccggtcttct tctaaagagt ctttggtacg aacatcttcc agagaatccc tgaatcgact
      661 tgacctggac agttctactg ccagttttga tccaccctct gatatggata gcgaggctga
      721 agacttggta gggaattcag acagtctcaa caaagaacag ttgattcagc ggttgcgaag
      781 aatggaacga agcttaagta gctacagggg aaaatattct gagcttgtta cagcttatca
      841 gatgcttcag agagagaaga aaaagctaca aggtatatta agtcagagtc aggataaatc
      901 acttcggaga atagcagaat taagagagga gctccaaatg gaccagcagg caaagaaaca
      961 tctgcaagag gagtttgatg catctttaga ggagaaagat cagtatatca gtgttctcca
     1021 aactcaggtt tctctactga aacaacgatt acgaaatggc ccgatgaatg ttgatgtact
     1081 gaaaccactt cctcagctgg aaccacaggc tgaagtcttc actaaagaag agaatccaga
     1141 aagtgatgga gagccagtag tggaagatgg aacttctgta aaaacactgg aaacactcca
     1201 gcaaagagtg aagcgtcaag agaacctact taagcgttgt aaggaaacaa ttcagtcaca
     1261 taaggaacaa tgtacactat taactagtga aaaagaagct ctgcaagaac aactggatga
     1321 aagacttcaa gaactagaaa agataaagga ccttcatatg gccgagaaga ctaaacttat
     1381 cactcagttg cgtgatgcaa agaacttaat tgaacagctt gaacaagata agggaatggt
     1441 aatcgcagag acaaaacgtc agatgcatga aaccctggaa atgaaagaag aagaaattgc
     1501 tcaactccgt agtcgcatca aacagatgac tacccaggga gaggaattac gggaacagaa
     1561 agaaaagtcc gaaagagctg cttttgagga acttgaaaaa gctttgagta cagcccaaaa
     1621 aacagaggaa gcacggagaa aactgaaggc agaaatggat gaacaaataa aaactatcga
     1681 aaaaacaagt gaggaggaac gcatcagtct tcaacaggaa ttaagtcggg tgaaacagga
     1741 ggttgttgat gtaatgaaaa aatcctcaga agaacaaatt gctaagctac agaagcttca
     1801 tgaaaaggag ctggccagaa aagagcagga actgaccaag aagcttcaga cccgagaaag
     1861 ggaatttcag gaacaaatga aagtagctct tgaaaagagt caatcagaat atttgaagat
     1921 cagccaagaa aaagaacagc aagaatcttt ggccctagaa gagttagagt tgcagaaaaa
     1981 agcaatcctc acagaaagtg aaaataaact tcgggacctt cagcaagaag cagagactta
     2041 cagaactaga attcttgaat tggaaagttc tttggaaaaa agcttacaag aaaacaaaaa
     2101 tcagtcaaaa gatttggctg ttcatctgga agctgaaaaa aataagcaca ataaggagat
     2161 tacagtcatg gttgaaaaac acaagacaga attggaaagc cttaagcatc agcaggatgc
     2221 cctttggact gaaaaactcc aagtcttaaa gcaacaatat cagactgaaa tggaaaaact
     2281 tagggaaaag tgtgaacaag aaaaagaaac attgttgaaa gacaaagaga ttatcttcca
     2341 ggcccacata gaagaaatga atgaaaagac tttagaaaag cttgatgtga agcaaacaga
     2401 actagaatca ttatcttctg aactgtcaga agtattaaaa gcccgtcaca aactagaaga
     2461 ggaactttct gttctgaaag atcaaacaga taaaatgaag caggaattag aggccaagat
     2521 ggatgaacag aaaaatcatc accagcagca agttgacagt atcattaaag aacacgaggt
     2581 atctatccag aggactgaga aggcattaaa agatcaaatt aatcaacttg agcttctctt
     2641 gaaggaaagg gacaagcatt tgaaagagca tcaggctcat gtagaaaatt tagaggcaga
     2701 tattaaaagg tctgaagggg aactccagca ggcatctgct aagctggacg tttttcagtc
     2761 ttaccagagt gccacacatg agcagacaaa agcatatgag gaacagttgg cccaattgca
     2821 gcagaagttg ttggatttgg aaacagaaag aattcttctt accaaacagg ttgctgaagt
     2881 tgaagcacaa aagaaagatg tttgtactga gttagatgct cacaaaatcc aggtgcagga
     2941 cttaatgcag caacttgaaa aacaaaatag tgaaatggag caaaaagtaa aatctttaac
     3001 ccaagtctat gagtccaaac ttgaagatgg taacaaagaa caggaacaga caaagcaaat
     3061 cttggtggaa aaggaaaata tgattttaca aatgagagaa ggacagaaga aagaaattga
     3121 gatactcaca cagaaattgt cagccaagga ggacagtatt catattttga atgaggaata
     3181 tgaaaccaaa tttaaaaacc aagaaaaaaa gatggaaaaa gttaagcaga aagcaaagga
     3241 gatgcaagaa acgttaaaga aaaaattact ggatcaggaa gccaaactta agaaagagct
     3301 tgaaaatact gctctagagc ttagtcagaa agaaaaacag tttaatgcca aaatgctgga
     3361 aatggcacag gctaactcag ctggaatcag tgatgcagtg tcaagactgg aaacaaacca
     3421 aaaagaacaa atagaaagtc ttactgaggt tcatcgacga gaactcaatg atgtcatatc
     3481 aatctgggaa aagaaactta atcagcaagc tgaagaactt caggaaatac atgaaatcca
     3541 attacaggaa aaagaacaag aggtagcaga actgaaacaa aagatcctcc tatttgggtg
     3601 tgaaaaagaa gagatgaaca aggaaataac atggctgaag gaagaaggtg ttaagcagga
     3661 tacaacatta aatgaattac aggaacagtt aaagcagaag tctgcccatg tgaattctct
     3721 tgcacaagat gaaactaaac tgaaagctca tcttgaaaag ctagaggttg acttgaataa
     3781 gtctctgaag gaaaatactt ttcttcaaga gcagctagtt gaactgaaga tgctggcaga
     3841 agaagataag cggaaggttt ctgagttgac tagcaagttg aaaaccacag atgaagaatt
     3901 ccagagtttg aaatcttcac atgaaaaaag taacaaaagc ctagaggaca agagcttgga
     3961 atttaaaaaa ctgtctgagg aactagcgat tcagctagat atttgctgta agaaaaccga
     4021 agccttatta gaagctaaaa caaatgagct aatcaacatt agtagtagta aaactaatgc
     4081 cattctttct aggatttctc attgtcagca ccgtacaact aaagttaagg aggcactgtt
     4141 aattaaaact tgcacagttt ctgaattaga agcacaactt agacagttga cagaggagca
     4201 aaatacacta aatatttctt ttcaacaggc tactcatcag ttagaagaaa aagaaaatca
     4261 aattaagagc atgaaggctg atattgaaag tcttgtaaca gaaaaagaag ccttacagaa
     4321 ggaaggaggc aatcagcaac aggctgcttc tgaaaaggag tcttgtataa cacagttgaa
     4381 gaaagagtta tctgaaaaca tcaatgctgt cacattgatg aaagaagagc ttaaagaaaa
     4441 aaaagttgag attagcagtc ttagtaaaca actaactgat ttgaatgttc agcttcaaaa
     4501 tagcatcagc ctatccgaaa aagaagcagc catttcatca ctaagaaagc agtatgatga
     4561 agaaaaatgt gaattgctgg atcaggtgca agatttatct tttaaagttg acactctgag
     4621 taaagagaaa atttctgctc ttgagcaggt agatgactgg tccaataaat tctcagaatg
     4681 gaagaagaaa gcacagtcaa gatttacaca gcatcaaaac actgttaaag aattgcagat
     4741 ccagcttgag ttaaaatcaa aggaagctta tgaaaaggat gagcagataa atttattgaa
     4801 ggaagagctt gatcagcaaa ataaaagatt tgattgttta aagggtgaaa tggaagacga
     4861 caagagcaag atggagaaaa aggagtctaa tttagaaaca gagttaaagt ctcaaacagc
     4921 aagaattatg gaattagagg accatattac ccagaaaact attgaaatag agtccttaaa
     4981 tgaagttctt aaaaattaca atcaacaaaa ggatattgaa cacaaagaat tggttcagaa
     5041 acttcaacat tttcaagagt taggagaaga aaaggacaac agggttaaag aagctgaaga
     5101 aaaaatctta acacttgaaa accaagttta ttccatgaaa gctgaacttg aaactaagaa
     5161 gaaagaatta gaacatgtga atttaagtgt gaaaagcaaa gaggaggagt taaaggcatt
     5221 ggaagatagg cttgagtcag aaagtgctgc aaaattagca gagttgaaga gaaaagctga
     5281 acaaaaaatt gctgccatta agaagcagtt gttatctcaa atggaagaga aagaagaaca
     5341 gtataaaaaa ggtacagaaa gccatttgag tgagctaaat acaaaattgc aggaaagaga
     5401 aagggaagtt cacatcttgg aagaaaaact taagtcagtg gaaagttcac agtcagaaac
     5461 attaattgta cccagatcag caaaaaatgt ggcagcatat actgaacaag aagaagcaga
     5521 ttcccaaggc tgtgtgcaga agacatatga agaaaaaatc agtgttttac aaagaaactt
     5581 aactgaaaaa gaaaagctat tgcagagggt agggcaggaa aaagaagaga cagtttcttc
     5641 tcattttgaa atgcgatgcc aataccagga gcgcttaata aagctagaac atgctgaggc
     5701 aaagcaacat gaagatcaaa gtatgatagg tcatcttcaa gaggagcttg aagaaaaaaa
     5761 caagaaatat tccttgatag tagcccagca tgtggaaaaa gaaggaggta aaaataacat
     5821 acaggcaaag caaaacttgg aaaatgtgtt tgacgacgtc cagaaaaccc tccaggagaa
     5881 ggaactaacc tgtcagattt tggagcaaaa gataaaagag ctggattcct gcttagtaag
     5941 acagaaagaa gtacatagag ttgaaatgga agagttgacc tcaaaatatg aaaaattaca
     6001 ggctttacaa cagatggatg gaagaaataa acccacagaa cttttggaag aaaacactga
     6061 agaaaagtcc aaatcacatt tggtccaacc caaattgctt agtaacatgg aagcccagca
     6121 caatgatctg gagtttaaat tagccggggc agaacgggag aaacagaaac tgggcaagga
     6181 gattgttaga ttgcagaaag accttcgaat gttgagaaag gagcatcagc aagaattgga
     6241 aatactaaag aaagaatatg atcaagaaag ggaagagaaa atcaaacagg agcaggaaga
     6301 tcttgaactg aagcacaatt ccacattaaa acagctgatg agggagttta atacacagct
     6361 ggcacaaaag gaacaagagc tggaaatgac cataaaagaa actatcaata aggcccagga
     6421 ggtggaggct gaacttttag aaagccatca agaagagaca aatcagttac ttaaaaaaat
     6481 tgctgagaaa gatgatgatc taaaacgaac agccaaaaga tatgaagaaa tccttgatgc
     6541 tcgtgaagaa gaaatgactg caaaagtaag ggacctgcag actcaacttg aggagctgca
     6601 gaagaaatac cagcaaaagc tagagcagga ggagaaccct ggcaatgata atgtaacaat
     6661 tatggagcta cagacacagc tagcacagaa gacgacttta atcagtgatt cgaaattgaa
     6721 agagcaagag ttcagagaac agattcacaa tttagaagac cgtttgaaga aatatgaaaa
     6781 gaatgtatat gcaacaactg tggggacacc ttacaaaggt ggcaatttgt accatacgga
     6841 tgtctcactc tttggagaac ctaccgaatt tgagtatttg cgaaaagtgc tttttgagta
     6901 tatgatgggt cgtgagacta agaccatggc aaaagttata accaccgtac tgaagttccc
     6961 tgatgatcag actcagaaaa ttttggaaag agaagatgct cggctgatgt catggctccg
     7021 atcttcatct tgaagaagag tgacattggg tgactgctgc ttggaaaact gtccacactt
     7081 gctactcttt gagaatgaag ttgtcattca gggcccctca tgtagccaaa agaccaagaa
     7141 aaatctggcc cacagataag ttgcagactg cctttaaaat agattttatc agtggagaaa
     7201 tggtgatagt tttttcttca gttttctctt gggaagagtt ttatgttgtt taaaagatat
     7261 tttgataact taacctgctt tatgggctta cataatattc ctttcatcca ttctttttaa
     7321 agaacggctt acctttccta tttattttta gggtgatttt ttaaaaagac ttgtgcaata
     7381 cattttgagg tgaaacttag tggatttttt ctgataaatt agagcattta attgactatt
     7441 ttattcaggt tgatctgttg aatatttgct aaagaccagt tctttaagct aagacatgta
     7501 aaaaatccca aatggcagta cctcattgtt tacttagctt ttgtacttat atttttcaga
     7561 ggaaaaaaca ctactgtaaa ttgtgaatag ccaatacata actgtattgt atgcaaatct
     7621 gtgattgttg gcagtgtcat ctctgagaaa cagataaata aagtttattt actatataac
     7681 atttgtttat tgtacttgtt cctcagttga aatctatttt aaaatgttta agaatgcata
     7741 ttctatttgg atgaatttag tgaggaatta tttgtcaata taactaaatg ctgagcattt
     7801 gaaaagaat
//
