LOCUS       NM_080425               4027 bp    mRNA    linear   PRI 18-OCT-2024
DEFINITION  Homo sapiens GNAS complex locus (GNAS), transcript variant 2, mRNA.
ACCESSION   NM_080425
VERSION     NM_080425.4
KEYWORDS    RefSeq; MANE Plus Clinical.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Qi,J., Huang,Y. and Bian,Y.
  TITLE     GNAS, not a Highly Mutated Gene, Has Prognostic Significance and
            Carcinogenic Effects in Osteosarcoma
  JOURNAL   Cell Physiol Biochem 58 (5), 510-526 (2024)
   PUBMED   39305132
  REMARK    GeneRIF: GNAS, not a Highly Mutated Gene, Has Prognostic
            Significance and Carcinogenic Effects in Osteosarcoma.
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Crane,J.L., Shamblott,M.J., Axelman,J., Hsu,S., Levine,M.A. and
            Germain-Lee,E.L.
  TITLE     Imprinting status of Galpha(s), NESP55, and XLalphas in cell
            cultures derived from human embryonic germ cells: GNAS imprinting
            in human embryonic germ cells
  JOURNAL   Clin Transl Sci 2 (5), 355-360 (2009)
   PUBMED   20443919
  REMARK    GeneRIF: analysis of the imprinting Status of Galpha(s), NESP55,
            and XLalphas in cell cultures derived from human embryonic germ
            cells
REFERENCE   3  (bases 1 to 4027)
  AUTHORS   Bastepe,M.
  TITLE     The GNAS Locus: Quintessential Complex Gene Encoding Gsalpha,
            XLalphas, and other Imprinted Transcripts
  JOURNAL   Curr Genomics 8 (6), 398-414 (2007)
   PUBMED   19412439
  REMARK    GeneRIF: GNAS locus has a highly complex imprinted expression
            pattern, giving rise to transcripts (including non-coding) that are
            maternally, paternally, or biallelically expressed.
REFERENCE   4  (bases 1 to 4027)
  AUTHORS   Jakobsen,A.M., Ahlman,H., Kolby,L., Abrahamsson,J.,
            Fischer-Colbrie,R. and Nilsson,O.
  TITLE     NESP55, a novel chromogranin-like peptide, is expressed in
            endocrine tumours of the pancreas and adrenal medulla but not in
            ileal carcinoids
  JOURNAL   Br J Cancer 88 (11), 1746-1754 (2003)
   PUBMED   12771991
  REMARK    GeneRIF: Neuroendocrine secretory protein 55 is found in a subset
            of neuroendocrine tumours showing differentiation towards adrenal
            chromaffin cells and pancreatic islets cells and not in ileal
            carcinoids
REFERENCE   5  (bases 1 to 4027)
  AUTHORS   Freson,K., Jaeken,J., Van Helvoirt,M., de Zegher,F.,
            Wittevrongel,C., Thys,C., Hoylaerts,M.F., Vermylen,J. and Van
            Geet,C.
  TITLE     Functional polymorphisms in the paternally expressed XLalphas and
            its cofactor ALEX decrease their mutual interaction and enhance
            receptor-mediated cAMP formation
  JOURNAL   Hum Mol Genet 12 (10), 1121-1130 (2003)
   PUBMED   12719376
REFERENCE   6  (bases 1 to 4027)
  AUTHORS   Weiss,U., Ischia,R., Eder,S., Lovisetti-Scamihorn,P., Bauer,R. and
            Fischer-Colbrie,R.
  TITLE     Neuroendocrine secretory protein 55 (NESP55): alternative splicing
            onto transcripts of the GNAS gene and posttranslational processing
            of a maternally expressed protein
  JOURNAL   Neuroendocrinology 71 (3), 177-186 (2000)
   PUBMED   10729789
REFERENCE   7  (bases 1 to 4027)
  AUTHORS   Ischia,R., Lovisetti-Scamihorn,P., Hogue-Angeletti,R.,
            Wolkersdorfer,M., Winkler,H. and Fischer-Colbrie,R.
  TITLE     Molecular cloning and characterization of NESP55, a novel
            chromogranin-like precursor of a peptide with 5-HT1B receptor
            antagonist activity
  JOURNAL   J Biol Chem 272 (17), 11657-11662 (1997)
   PUBMED   9111083
REFERENCE   8  (bases 1 to 4027)
  AUTHORS   Haldeman-Englert,C.R., Hurst,A.C.E. and Levine,M.A.
  TITLE     Disorders of GNAS Inactivation
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   29072892
REFERENCE   9  (bases 1 to 4027)
  AUTHORS   Szymczuk,V., Florenzano,P., de Castro,L.F., Collins,M.T. and
            Boyce,A.M.
  TITLE     Fibrous Dysplasia / McCune-Albright Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   25719192
REFERENCE   10 (bases 1 to 4027)
  AUTHORS   Weinstein,L.S., Gejman,P.V., de Mazancourt,P., American,N. and
            Spiegel,A.M.
  TITLE     A heterozygous 4-bp deletion mutation in the Gs alpha gene (GNAS1)
            in a patient with Albright hereditary osteodystrophy
  JOURNAL   Genomics 13 (4), 1319-1321 (1992)
   PUBMED   1505964
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL132655.13, CN295544.1,
            X04408.1 and BQ029639.1.
            
            On Aug 4, 2020 this sequence version replaced NM_080425.3.
            
            Summary: This locus has a highly complex imprinted expression
            pattern. It gives rise to maternally, paternally, and biallelically
            expressed transcripts that are derived from four alternative
            promoters and 5' exons. Some transcripts contain a differentially
            methylated region (DMR) at their 5' exons, and this DMR is commonly
            found in imprinted genes and correlates with transcript expression.
            An antisense transcript is produced from an overlapping locus on
            the opposite strand. One of the transcripts produced from this
            locus, and the antisense transcript, are paternally expressed
            noncoding RNAs, and may regulate imprinting in this region. In
            addition, one of the transcripts contains a second overlapping ORF,
            which encodes a structurally unrelated protein - Alex. Alternative
            splicing of downstream exons is also observed, which results in
            different forms of the stimulatory G-protein alpha subunit, a key
            element of the classical signal transduction pathway linking
            receptor-ligand interactions with the activation of adenylyl
            cyclase and a variety of cellular reponses. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            Mutations in this gene result in pseudohypoparathyroidism type 1a,
            pseudohypoparathyroidism type 1b, Albright hereditary
            osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright
            syndrome, progressive osseus heteroplasia, polyostotic fibrous
            dysplasia of bone, and some pituitary tumors. [provided by RefSeq,
            Aug 2012].
            
            Transcript Variant: This variant (2, also known as GNASXL) is
            paternally expressed and includes an alternate 5' exon, compared to
            variant 1. This variant includes two overlapping open reading
            frames encoding isoforms XLas and Alex, respectively. This RefSeq
            represents isoform XLas (also known as alpha sXXL), which is a
            neuroendocrine-specific G-protein alpha subunit. The encoded
            isoform has a distinct and longer N-terminus, compared to isoform
            GNASL. Variants 2 and 12 both encode the same isoform (Alex).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.318060.1,
                                           SRR18074969.1783765.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene     :: PMID: 19412439
            MANE Ensembl match :: ENST00000371100.9/ ENSP00000360141.3
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2391              AL132655.13        119336-121726
            2392-2618           CN295544.1         1-227
            2619-4025           X04408.1           208-1614
            4026-4027           BQ029639.1         21-22               c
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.32"
     gene            1..4027
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /note="GNAS complex locus"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="MIM:139320"
     exon            1..2618
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     CDS             551..3664
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /note="isoform XLas is encoded by transcript variant 2;
                     secretogranin VI; G protein subunit alpha S; guanine
                     nucleotide binding protein (G protein), alpha stimulating
                     activity polypeptide 1; guanine nucleotide regulatory
                     protein; adenylate cyclase-stimulating G alpha protein;
                     protein ALEX; extra large alphas protein; alternative gene
                     product encoded by XL-exon"
                     /codon_start=1
                     /product="protein GNAS isoform XLas"
                     /protein_id="NP_536350.2"
                     /db_xref="CCDS:CCDS46622.1"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="MIM:139320"
                     /translation="MGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPS
                     PAEEMETEPPHNEPIPVENDGEACGPPEVSRPNFQVLNPAFREAGAHGSYSPPPEEAM
                     PFEAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEE
                     AMPFEFDQPAQRGCSQLLLQVPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSP
                     PPEETMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSAVRLTPAANAPPLW
                     VPGAIGSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVDDTPVNMDSPPIALDG
                     PPIKVSGAPDKRERAERPPVEEEAAEMEGAADAAEGGKVPSPGYGSPAAGAASADTAA
                     RAAPAAPADPDSGATPEDPDSGTAPADPDSGAFAADPDSGAAPAAPADPDSGAAPDAP
                     ADPDSGAAPDAPADPDAGAAPEAPAAPAAAETRAAHVAPAAPDAGAPTAPAASATRAA
                     QVRRAASAAPASGARRKIHLRPPSPEIQAADPPTPRPTRASAWRGKSESSRGRRVYYD
                     EGVASSDDDSSGDESDDGTSGCLRWFQHRRNRRRRKPQRNLLRNFLVQAFGGCFGRSE
                     SPQPKASRSLKVKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGYMC
                     THRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKN
                     NLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWE
                     DEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQ
                     VDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALN
                     LFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPG
                     EDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMH
                     LRQYELL"
     exon            2619..2691
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            2692..2736
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..2791
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..2911
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            2912..3009
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3010..3064
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3138
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3197
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3198..3318
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3319..3449
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3517
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     exon            3518..4027
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4002..4007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /note="hexamer: AATAAA"
     regulatory      4009..4014
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /note="hexamer: ATTAAA"
     polyA_site      4024
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /note="major polyA site"
     polyA_site      4027
                     /gene="GNAS"
                     /gene_synonym="AHO; AIMAH1; C20orf45; GNAS1; GPSA; GSA;
                     GSP; NESP; PITA3; POH; SCG6; SgVI"
                     /note="major polyA site"
ORIGIN      
        1 agactccaaa tcagtcaggg agaggagtgg aaggagccaa aacgtccctg cgaggcctgc
       61 gctcaccagc ccccttaccc aaagtcccgg gcccctggcg ccaggagcag gctcctggag
      121 ccggagccag ctcggcactg gaaccggcgt cctctggtgg cagagagaga gcgctactgg
      181 cgattttcgg accgaatcgg cacgctcgtc agatccaagc aggcgggact ggcctggagc
      241 aaaaagaaag agagaggagg gcgtaaggat agaccaagga agaggggctg gggggcagcc
      301 tgggggcatg aaaagtggcc aggaaggagc caagactcca ccagcaacaa ttgagttgct
      361 tcagcctcag tctagggttc cttccaggcc ttgaaccccc caacctcaca agggttggaa
      421 agtgaggccg gtgaactttc cagctggtac tttgatttta aaataataat aataattttt
      481 tcaccctagt tcggttgggt gctccatctt acggagcccc aaacttattt tgagaggccg
      541 ccaccgtgtt atgggcgtgc gcaactgcct ctacggcaat aatatgtcag gacaacgcga
      601 tatcccccct gaaatcgggg aacagcccga gcaaccacct ttggaggccc caggggcagc
      661 tgcccccggt gctgggccta gcccagccga agagatggag accgaaccgc ctcacaacga
      721 gcccatcccc gtcgagaatg atggcgaggc ctgtggaccc ccagaggtct ccagacccaa
      781 ctttcaggtc ctcaacccgg cattcaggga agctggagcc catggaagct acagcccacc
      841 tcctgaggaa gcaatgccct tcgaggctga acagcccagc ttgggaggct tctggcctac
      901 actggagcag cctggattcc ccagtggggt ccatgcaggc cttgaggcct tcggcccagc
      961 actcatggag cccggagcct tcagtggtgc cagaccaggc ctgggaggat acagccctcc
     1021 accagaagaa gctatgccct ttgagtttga ccagcctgcc cagagaggct gcagtcaact
     1081 tctcttacag gtcccagacc ttgctccagg aggcccaggt gctgcagggg tccccggagc
     1141 tcctcccgag gagccccaag ccctcaggcc tgcaaaggct ggctccagag gaggctacag
     1201 ccctccccct gaggagacta tgccatttga gcttgatgga gaaggatttg gggacgacag
     1261 cccacccccg gggctttccc gagttatcgc acaagtcgac ggcagcagcc agttcgcggc
     1321 agtcgcggcc tcgagtgcgg tccgcctcac tcccgccgcg aacgcgcctc ccctctgggt
     1381 cccaggcgcc atcggcagcc catcccaaga ggctgtcaga cctccttcta acttcacggg
     1441 cagcagcccc tggatggaga tctccggacc cccgttcgag attggcagcg cccccgctgg
     1501 ggtcgacgac actcccgtca acatggacag ccccccaatc gcgcttgacg gcccgcccat
     1561 caaggtctcc ggagccccag ataagagaga gcgagcagag agacccccag ttgaggagga
     1621 agcagcagag atggaaggag ccgctgatgc cgcggaggga ggaaaagtac cctctccggg
     1681 gtacggatcc cctgccgccg gggcagcctc agcggatacc gctgccaggg cagcccctgc
     1741 agccccagcc gatcctgact ccggggcaac cccagaagat cccgactccg ggacagcacc
     1801 agccgatcct gactccgggg cattcgcagc cgatcccgac tccggggcag cccctgccgc
     1861 cccagccgat cccgactccg gggcggcccc tgacgcccca gccgatcccg actccggggc
     1921 ggcccctgac gccccagccg atccagatgc cggggcggcc cctgaggctc ccgccgcccc
     1981 tgcggctgct gagacccggg cagcccatgt cgccccagct gcgccagacg caggggctcc
     2041 cactgcccca gccgcttctg ccacccgggc agcccaagtc cgccgggcgg cctctgcagc
     2101 ccctgcctcc ggggccagac gcaagatcca tctcagaccc cccagccccg agatccaggc
     2161 tgccgatccg cctactccgc ggcctactcg cgcgtctgcc tggcggggca agtccgagag
     2221 cagccgcggc cgccgcgtgt actacgatga aggggtggcc agcagcgacg atgactccag
     2281 cggagacgag tccgacgatg ggacctccgg atgcctccgc tggtttcagc atcggcgaaa
     2341 tcgccgccgc cgaaagcccc agcgcaactt actccgcaac tttctcgtgc aagccttcgg
     2401 gggctgcttc ggtcgatctg agagtcccca gcccaaagcc tcgcgctctc tcaaggtcaa
     2461 gaaggtaccc ctggcggaga agcgcagaca gatgcgcaaa gaagccctgg agaagcgggc
     2521 ccagaagcgc gcagagaaga aacgcagtaa gctcatcgac aaacaactcc aggacgaaaa
     2581 gatgggctac atgtgtacgc accgcctgct gcttctaggt gctggagaat ctggtaaaag
     2641 caccattgtg aagcagatga ggatcctgca tgttaatggg tttaatggag agggcggcga
     2701 agaggacccg caggctgcaa ggagcaacag cgatggtgag aaggcaacca aagtgcagga
     2761 catcaaaaac aacctgaaag aggcgattga aaccattgtg gccgccatga gcaacctggt
     2821 gccccccgtg gagctggcca accccgagaa ccagttcaga gtggactaca tcctgagtgt
     2881 gatgaacgtg cctgactttg acttccctcc cgaattctat gagcatgcca aggctctgtg
     2941 ggaggatgaa ggagtgcgtg cctgctacga acgctccaac gagtaccagc tgattgactg
     3001 tgcccagtac ttcctggaca agatcgacgt gatcaagcag gctgactatg tgccgagcga
     3061 tcaggacctg cttcgctgcc gtgtcctgac ttctggaatc tttgagacca agttccaggt
     3121 ggacaaagtc aacttccaca tgtttgacgt gggtggccag cgcgatgaac gccgcaagtg
     3181 gatccagtgc ttcaacgatg tgactgccat catcttcgtg gtggccagca gcagctacaa
     3241 catggtcatc cgggaggaca accagaccaa ccgcctgcag gaggctctga acctcttcaa
     3301 gagcatctgg aacaacagat ggctgcgcac catctctgtg atcctgttcc tcaacaagca
     3361 agatctgctc gctgagaaag tccttgctgg gaaatcgaag attgaggact actttccaga
     3421 atttgctcgc tacactactc ctgaggatgc tactcccgag cccggagagg acccacgcgt
     3481 gacccgggcc aagtacttca ttcgagatga gtttctgagg atcagcactg ccagtggaga
     3541 tgggcgtcac tactgctacc ctcatttcac ctgcgctgtg gacactgaga acatccgccg
     3601 tgtgttcaac gactgccgtg acatcattca gcgcatgcac cttcgtcagt acgagctgct
     3661 ctaagaaggg aacccccaaa tttaattaaa gccttaagca caattaatta aaagtgaaac
     3721 gtaattgtac aagcagttaa tcacccacca tagggcatga ttaacaaagc aacctttccc
     3781 ttcccccgag tgattttgcg aaaccccctt ttcccttcag cttgcttaga tgttccaaat
     3841 ttagaaagct taaggcggcc tacagaaaaa ggaaaaaagg ccacaaaagt tccctctcac
     3901 tttcagtaaa aataaataaa acagcagcag caaacaaata aaatgaaata aaagaaacaa
     3961 atgaaataaa tattgtgttg tgcagcatta aaaaaaatca aaataaaaat taaatgtgag
     4021 caaagaa
//
