LOCUS       NM_007353               4369 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens G protein subunit alpha 12 (GNA12), transcript variant
            1, mRNA.
ACCESSION   NM_007353 XM_926881 XM_935570 XM_942577
VERSION     NM_007353.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Chaim,O.M., Miki,S., Prager,B.C., Ma,J., Jeong,A.Y., Lara,J.,
            Tran,N.K., Smith,J.M., Rich,J.N., Gutkind,J.S., Miyamoto,S.,
            Furnari,F.B. and Brown,J.H.
  TITLE     Galpha12 signaling regulates transcriptional and phenotypic
            responses that promote glioblastoma tumor invasion
  JOURNAL   Sci Rep 13 (1), 22412 (2023)
   PUBMED   38104152
  REMARK    GeneRIF: Galpha12 signaling regulates transcriptional and
            phenotypic responses that promote glioblastoma tumor invasion.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Yun,J., Kim,Y.S., Heo,M.J., Kim,M.J., Moon,A. and Kim,S.G.
  TITLE     ERalpha inhibits mesenchymal and amoeboidal movement of liver
            cancer cell via Galpha12
  JOURNAL   Int J Cancer 150 (10), 1690-1705 (2022)
   PUBMED   35020952
  REMARK    GeneRIF: ERalpha inhibits mesenchymal and amoeboidal movement of
            liver cancer cell via Galpha12.
REFERENCE   3  (bases 1 to 4369)
  AUTHORS   Gismondi,A., Nanni,V., Monteleone,V., Colao,C., Di Marco,G. and
            Canini,A.
  TITLE     Plant miR171 modulates mTOR pathway in HEK293 cells by targeting
            GNA12
  JOURNAL   Mol Biol Rep 48 (1), 435-449 (2021)
   PUBMED   33386590
  REMARK    GeneRIF: Plant miR171 modulates mTOR pathway in HEK293 cells by
            targeting GNA12.
REFERENCE   4  (bases 1 to 4369)
  AUTHORS   Mathiasen,S., Palmisano,T., Perry,N.A., Stoveken,H.M.,
            Vizurraga,A., McEwen,D.P., Okashah,N., Langenhan,T., Inoue,A.,
            Lambert,N.A., Tall,G.G. and Javitch,J.A.
  TITLE     G12/13 is activated by acute tethered agonist exposure in the
            adhesion GPCR ADGRL3
  JOURNAL   Nat Chem Biol 16 (12), 1343-1350 (2020)
   PUBMED   32778842
  REMARK    GeneRIF: G12/13 is activated by acute tethered agonist exposure in
            the adhesion GPCR ADGRL3.
            Erratum:[Nat Chem Biol. 2020 Dec;16(12):1440. doi:
            10.1038/s41589-020-0649-z.. PMID: 32807971]
REFERENCE   5  (bases 1 to 4369)
  AUTHORS   Stecky,R.C., Quick,C.R., Fleming,T.L., Mull,M.L., Vinson,V.K.,
            Whitley,M.S., Dover,E.N. and Meigs,T.E.
  TITLE     Divergent C-terminal motifs in Galpha12 and Galpha13 provide
            distinct mechanisms of effector binding and SRF activation
  JOURNAL   Cell Signal 72, 109653 (2020)
   PUBMED   32330601
  REMARK    GeneRIF: Divergent C-terminal motifs in Galpha12 and Galpha13
            provide distinct mechanisms of effector binding and SRF activation.
REFERENCE   6  (bases 1 to 4369)
  AUTHORS   Kabouridis,P.S., Waters,S.T., Escobar,S., Stanners,J. and
            Tsoukas,C.D.
  TITLE     Expression of GTP-binding protein alpha subunits in human
            thymocytes
  JOURNAL   Mol Cell Biochem 144 (1), 45-51 (1995)
   PUBMED   7791744
REFERENCE   7  (bases 1 to 4369)
  AUTHORS   Kozasa,T. and Gilman,A.G.
  TITLE     Purification of recombinant G proteins from Sf9 cells by
            hexahistidine tagging of associated subunits. Characterization of
            alpha 12 and inhibition of adenylyl cyclase by alpha z
  JOURNAL   J Biol Chem 270 (4), 1734-1741 (1995)
   PUBMED   7829508
REFERENCE   8  (bases 1 to 4369)
  AUTHORS   Kleuss,C., Raw,A.S., Lee,E., Sprang,S.R. and Gilman,A.G.
  TITLE     Mechanism of GTP hydrolysis by G-protein alpha subunits
  JOURNAL   Proc Natl Acad Sci U S A 91 (21), 9828-9831 (1994)
   PUBMED   7937899
REFERENCE   9  (bases 1 to 4369)
  AUTHORS   Offermanns,S., Laugwitz,K.L., Spicher,K. and Schultz,G.
  TITLE     G proteins of the G12 family are activated via thromboxane A2 and
            thrombin receptors in human platelets
  JOURNAL   Proc Natl Acad Sci U S A 91 (2), 504-508 (1994)
   PUBMED   8290554
REFERENCE   10 (bases 1 to 4369)
  AUTHORS   Chan,A.M., Fleming,T.P., McGovern,E.S., Chedid,M., Miki,T. and
            Aaronson,S.A.
  TITLE     Expression cDNA cloning of a transforming gene encoding the
            wild-type G alpha 12 gene product
  JOURNAL   Mol Cell Biol 13 (2), 762-768 (1993)
   PUBMED   8423800
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI553022.1, BM557907.1, AK127842.1, AK128701.1, AI381970.1 and
            CA446135.1.
            
            On Nov 23, 2018 this sequence version replaced NM_007353.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.197596.1,
                                           SRR1660805.167824.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000275364.8/ ENSP00000275364.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BI553022.1         27-57
            32-456              BM557907.1         24-448
            457-1633            AK127842.1         462-1638
            1634-3172           AK128701.1         1507-3045
            3173-3913           AK127842.1         3177-3917
            3914-4366           AI381970.1         1-453               c
            4367-4369           CA446135.1         13-15               c
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.3-p22.2"
     gene            1..4369
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /note="G protein subunit alpha 12"
                     /db_xref="GeneID:2768"
                     /db_xref="HGNC:HGNC:4380"
                     /db_xref="MIM:604394"
     exon            1..456
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..1293
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     guanine nucleotide-binding protein subunit alpha-12;
                     WUGSC:H_GS165O14.2; g alpha-12; guanine nucleotide binding
                     protein (G protein) alpha 12; heterotrimeric guanine
                     nucleotide-binding protein 1M1"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein subunit
                     alpha-12 isoform 1"
                     /protein_id="NP_031379.2"
                     /db_xref="CCDS:CCDS5335.1"
                     /db_xref="GeneID:2768"
                     /db_xref="HGNC:HGNC:4380"
                     /db_xref="MIM:604394"
                     /translation="MSGVVRTLSRCLLPAEAGGARERRAGSGARDAEREARRRSRDID
                     ALLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNIL
                     KGSRVLVDARDKLGIPWQYSENEKHGMFLMAFENKAGLPVEPATFQLYVPALSALWRD
                     SGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFVI
                     KKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNI
                     FETIVNNKLFFNVSIILFLNKMDLLVEKVKTVSIKKHFPDFRGDPHRLEDVQRYLVQC
                     FDRKRRNRSKPLFHHFTTAIDTENVRFVFHAVKDTILQENLKDIMLQ"
     exon            457..672
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /inference="alignment:Splign:2.1.0"
     exon            673..723
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /inference="alignment:Splign:2.1.0"
     exon            724..4369
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3896..3901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /note="hexamer: AATAAA"
     polyA_site      3915
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /note="major polyA site"
     regulatory      4337..4342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /note="hexamer: AATAAA"
     polyA_site      4369
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /note="major polyA site"
ORIGIN      
        1 ctcggagcga ctgcagcggc ggcggcggac gcggcctgag gcgagcggcg gggcgtgggg
       61 cggtgcctcg gcccgggctc gccctcgccg gcgggagcgt ccatggcccc cgggcgccgg
      121 cggggcgcgg ccgcggcctg aggggccatg tccggggtgg tgcggaccct cagccgctgc
      181 ctgctgccgg ccgaggccgg cggggcccgc gagcgcaggg cgggcagcgg cgcgcgcgac
      241 gcggagcgcg aggcccggag gcgtagccgc gacatcgacg cgctgctggc ccgcgagcgg
      301 cgcgcggtcc ggcgcctggt gaagatcctg ctgctgggcg cgggcgagag cggcaagtcc
      361 acgttcctca agcagatgcg catcatccac ggccgcgagt tcgaccagaa ggcgctgctg
      421 gagttccgcg acaccatctt cgacaacatc ctcaagggct caagggttct tgttgatgca
      481 cgagataagc ttggcattcc ttggcagtat tctgaaaatg agaagcatgg gatgttcctg
      541 atggccttcg agaacaaggc ggggctgcct gtggagccgg ccaccttcca gctgtacgtc
      601 ccggccctga gcgcactctg gagggattct ggcatcaggg aggctttcag ccggagaagc
      661 gagtttcagc tgggggagtc ggtgaagtac ttcctggaca acttggaccg gatcggccag
      721 ctgaattact ttcctagtaa gcaagatatc ctgctggcta ggaaagccac caagggaatt
      781 gtggagcatg acttcgttat taagaagatc ccctttaaga tggtggatgt gggcggccag
      841 cggtcccagc gccagaagtg gttccagtgc ttcgacggga tcacgtccat cctgttcatg
      901 gtctcctcca gcgagtacga ccaggtcctc atggaggaca ggcgcaccaa ccggctggtg
      961 gagtccatga acatcttcga gaccatcgtc aacaacaagc tcttcttcaa cgtctccatc
     1021 attctcttcc tcaacaagat ggacctcctg gtggagaagg tgaagaccgt gagcatcaag
     1081 aagcacttcc cggacttcag gggcgacccg cacaggctgg aggacgtcca gcgctacctg
     1141 gtccagtgct tcgacaggaa gagacggaac cgcagcaagc cactcttcca ccacttcacc
     1201 accgccatcg acaccgagaa cgtccgcttc gtgttccatg ctgtgaaaga caccatcctg
     1261 caggagaacc tgaaggacat catgctgcag tgagcgagga agccccgggg tttgtcgtcg
     1321 ttgagcagcc cccacggctg tcggtcagac tcttgggtgt gtgttgtctg tgtggtcctt
     1381 gagtgggttt ctcggatccg tgccctggaa tacctggctc aggaatgctg tcagaccagc
     1441 cagccagcga gctctaggca aaaggacatg gaaactgtca cgttagctac tgaatcctgg
     1501 gggcgagtga aactactgaa aatccgagtg atgatgttgt gaatacggaa cacctaatca
     1561 cacagcttgc tttgctttta cagaaacgtt cctctttttc tgacgcagtt taattgagga
     1621 ccgtgttgtg tgtgtatgtg tgtacacacg ctctgtcttt aatgacagaa acacaaaaac
     1681 cagctggcct tgcagacggc ttttctaact cacaagtctt ccctgagaca gactaacctg
     1741 aaagctttgc ctaacagtag cttgtagaga tccagtgcac gccgatgctg ctaaactcag
     1801 tgcctgagcc cggccctgca gccccagccg cagtgtctga aggccacctc ccaaagggag
     1861 cacgttgcct tttcaaactc ccgtgccgat ttcctaagag cccctagtcc aagcctctca
     1921 gatgaagctg aggagccgtg cctaggatcc cttcccagct ctgaggacgg gctgcagagc
     1981 tctgcaggtg tggattcacc ttacgcccct acagcaggct cagcccttcc caccctgccc
     2041 catgcccagc agcacaacac ggagtgagac aggatgccca cggtgactgc cgctccgtcc
     2101 gtgcacacac agcggtgctc ttctcccctt agccacccac tgcccaaccc aacggcaaag
     2161 acacagaaac caggtcccct tgcagacggc tctcccatct tcctgcaagt catctgctca
     2221 cacacagttg gcagcacata gcgtttcctt ctttcagaaa cattcctctt ctggggcttc
     2281 agaaagctgg caaggccact agcagagctt ttgttaatgc cccagctgct tggcgagcta
     2341 acagctgacc tttcgggaag cccacagacg ctggaggaat cttgagtttc tccaaactgc
     2401 cgctccacca gtgcctttgg acagccgtgc ctgttcgccg ctctccctaa gtctgattct
     2461 catcgaggcc cctcgcttct atgactgtgc ttgcagaaga gtaaacactc tcggatgccg
     2521 ctgtcctggg ggagcccgcg ggagcctgtg aatgttgata cgagctggcc agtcctgggc
     2581 ccagctcact tgtccagcta cctgccaggt ggctttcact gtgtttaaaa tacattgcat
     2641 tccaagctgg tcccctctgt gtatcactct actgagaaat cctgcctagt gtgttttggg
     2701 atgtgtccta gcatttacaa gaaaatgaaa agcgtcctct taattggcac ccgaatgttg
     2761 ctgtggctca gtcacatatc ccagggccct cgtcccgagg ccgtgctgcc ccgagccccg
     2821 agcccctctg cagctcaccc ttggcttgtt ttccgcaaac ccggtaaacg caagcccttg
     2881 gggcagatgc agaagcagaa gagggagggg aaacctgcct ctgggtcacc ctgttagcac
     2941 agcgttctca tcgggagaca gcatggaact ctctctcgca gtgctcgagg ctgtgtgtca
     3001 gtgtttgctg ggcttgtggc tccttttttg gctggataaa gaagtcgctg tttttgtact
     3061 gcttctgtgg ctcttcacag acctcacgga tgtgaccgga gatgagtgcc gatgaccacg
     3121 ttttaaagga gaaagagagc tcctggtggg gccctcgggg tggtctcagg tcccatttgc
     3181 agtctgcaac agtgacgcgc agcccggtcc ggagcgtggt gagctttgtt tgccttctgg
     3241 gtcagctttc gctgtgtctc ctgtgtgtgt tagaatccag agcccagagg aagtgcaagc
     3301 gggtcctccg ccaacgggga gagcctcttc gcggcgctgt tggcgacagc agcgctgtga
     3361 ttcgcgtagc aggggagttg tttgaaacac cttcctgagt agtccggcct tgtcaatgag
     3421 tgcttgtttt cctttaaaca gtctgacata tttactcgtc actttcaaac cagaagcatg
     3481 agaggaagga gatattgtgg ggtccgttta actcgataga aagcgcaggg ggatggcccc
     3541 cggcgcgggc tcttgacccg ctcagcgctg accccaccgc cctggccgag gcacttggcc
     3601 ttgctgagct ggacttcctc ctcctcctcc tcatgaccgg ggtgaattag aacgttttta
     3661 aagacacccc cttccaaatt ctgtaacaca ttgtaattgg agaagaagga aactctgcaa
     3721 ggctaaactg tcattcacaa cttggctaca catagactct agtcagtttt gtctccagaa
     3781 ccttaggctt ttgtattttt taattttaat ttcactgtta atccttattg tcttttttat
     3841 taagatgttg gaaaagcagg aggtagttgt gcctcaatta ttgcaaaaat gtaacaataa
     3901 agttcctcaa aataagatct gttcctcata gctatactgt gtacacataa gacgcatata
     3961 gggttttact gaaatctatt tttaactctt atgttcgtag agaaattgtt tcaaggattt
     4021 tgagtcatag gtctgtaatt tatagagatc tctagaattc ttattgtaat tttcctactt
     4081 ctttgataaa agaaaaataa gtcagattgt taactccaag attgaaaaaa aaaactcttg
     4141 aaagaagatt attagttgta actaatttag gggttctggg cacagacatc taacctggta
     4201 ttgtaaggca gaggctccca ttggaatggt agtggtccgg gtcagttgtt catggtgtaa
     4261 gctttgcaca gtgtattaac attgggaggg tctggcttga aaatttggcc accctcagcc
     4321 tctgaatgtt tattaaaata aatttagtct ttctttgctt aatataaaa
//
