LOCUS       NP_031379                381 aa            linear   PRI 27-APR-2025
DEFINITION  guanine nucleotide-binding protein subunit alpha-12 isoform 1 [Homo
            sapiens].
ACCESSION   NP_031379 XP_931974 XP_940663 XP_947670
VERSION     NP_031379.2
DBSOURCE    REFSEQ: accession NM_007353.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 381)
  AUTHORS   Chaim,O.M., Miki,S., Prager,B.C., Ma,J., Jeong,A.Y., Lara,J.,
            Tran,N.K., Smith,J.M., Rich,J.N., Gutkind,J.S., Miyamoto,S.,
            Furnari,F.B. and Brown,J.H.
  TITLE     Galpha12 signaling regulates transcriptional and phenotypic
            responses that promote glioblastoma tumor invasion
  JOURNAL   Sci Rep 13 (1), 22412 (2023)
   PUBMED   38104152
  REMARK    GeneRIF: Galpha12 signaling regulates transcriptional and
            phenotypic responses that promote glioblastoma tumor invasion.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 381)
  AUTHORS   Yun,J., Kim,Y.S., Heo,M.J., Kim,M.J., Moon,A. and Kim,S.G.
  TITLE     ERalpha inhibits mesenchymal and amoeboidal movement of liver
            cancer cell via Galpha12
  JOURNAL   Int J Cancer 150 (10), 1690-1705 (2022)
   PUBMED   35020952
  REMARK    GeneRIF: ERalpha inhibits mesenchymal and amoeboidal movement of
            liver cancer cell via Galpha12.
REFERENCE   3  (residues 1 to 381)
  AUTHORS   Gismondi,A., Nanni,V., Monteleone,V., Colao,C., Di Marco,G. and
            Canini,A.
  TITLE     Plant miR171 modulates mTOR pathway in HEK293 cells by targeting
            GNA12
  JOURNAL   Mol Biol Rep 48 (1), 435-449 (2021)
   PUBMED   33386590
  REMARK    GeneRIF: Plant miR171 modulates mTOR pathway in HEK293 cells by
            targeting GNA12.
REFERENCE   4  (residues 1 to 381)
  AUTHORS   Mathiasen,S., Palmisano,T., Perry,N.A., Stoveken,H.M.,
            Vizurraga,A., McEwen,D.P., Okashah,N., Langenhan,T., Inoue,A.,
            Lambert,N.A., Tall,G.G. and Javitch,J.A.
  TITLE     G12/13 is activated by acute tethered agonist exposure in the
            adhesion GPCR ADGRL3
  JOURNAL   Nat Chem Biol 16 (12), 1343-1350 (2020)
   PUBMED   32778842
  REMARK    GeneRIF: G12/13 is activated by acute tethered agonist exposure in
            the adhesion GPCR ADGRL3.
            Erratum:[Nat Chem Biol. 2020 Dec;16(12):1440. doi:
            10.1038/s41589-020-0649-z. PMID: 32807971]
REFERENCE   5  (residues 1 to 381)
  AUTHORS   Stecky,R.C., Quick,C.R., Fleming,T.L., Mull,M.L., Vinson,V.K.,
            Whitley,M.S., Dover,E.N. and Meigs,T.E.
  TITLE     Divergent C-terminal motifs in Galpha12 and Galpha13 provide
            distinct mechanisms of effector binding and SRF activation
  JOURNAL   Cell Signal 72, 109653 (2020)
   PUBMED   32330601
  REMARK    GeneRIF: Divergent C-terminal motifs in Galpha12 and Galpha13
            provide distinct mechanisms of effector binding and SRF activation.
REFERENCE   6  (residues 1 to 381)
  AUTHORS   Kabouridis,P.S., Waters,S.T., Escobar,S., Stanners,J. and
            Tsoukas,C.D.
  TITLE     Expression of GTP-binding protein alpha subunits in human
            thymocytes
  JOURNAL   Mol Cell Biochem 144 (1), 45-51 (1995)
   PUBMED   7791744
REFERENCE   7  (residues 1 to 381)
  AUTHORS   Kozasa,T. and Gilman,A.G.
  TITLE     Purification of recombinant G proteins from Sf9 cells by
            hexahistidine tagging of associated subunits. Characterization of
            alpha 12 and inhibition of adenylyl cyclase by alpha z
  JOURNAL   J Biol Chem 270 (4), 1734-1741 (1995)
   PUBMED   7829508
REFERENCE   8  (residues 1 to 381)
  AUTHORS   Kleuss,C., Raw,A.S., Lee,E., Sprang,S.R. and Gilman,A.G.
  TITLE     Mechanism of GTP hydrolysis by G-protein alpha subunits
  JOURNAL   Proc Natl Acad Sci U S A 91 (21), 9828-9831 (1994)
   PUBMED   7937899
REFERENCE   9  (residues 1 to 381)
  AUTHORS   Offermanns,S., Laugwitz,K.L., Spicher,K. and Schultz,G.
  TITLE     G proteins of the G12 family are activated via thromboxane A2 and
            thrombin receptors in human platelets
  JOURNAL   Proc Natl Acad Sci U S A 91 (2), 504-508 (1994)
   PUBMED   8290554
REFERENCE   10 (residues 1 to 381)
  AUTHORS   Chan,A.M., Fleming,T.P., McGovern,E.S., Chedid,M., Miki,T. and
            Aaronson,S.A.
  TITLE     Expression cDNA cloning of a transforming gene encoding the
            wild-type G alpha 12 gene product
  JOURNAL   Mol Cell Biol 13 (2), 762-768 (1993)
   PUBMED   8423800
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI553022.1, BM557907.1, AK127842.1, AK128701.1, AI381970.1 and
            CA446135.1.
            
            On or before Mar 28, 2006 this sequence version replaced
            XP_931974.1, XP_947670.1, XP_940663.1, NP_031379.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.197596.1,
                                           SRR1660805.167824.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000275364.8/ ENSP00000275364.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.3-p22.2"
     Protein         1..381
                     /product="guanine nucleotide-binding protein subunit
                     alpha-12 isoform 1"
                     /note="guanine nucleotide-binding protein subunit
                     alpha-12; WUGSC:H_GS165O14.2; g alpha-12; guanine
                     nucleotide binding protein (G protein) alpha 12;
                     heterotrimeric guanine nucleotide-binding protein 1M1"
                     /calculated_mol_wt=44148
     Region          42..379
                     /region_name="G_alpha"
                     /note="G protein alpha subunit; smart00275"
                     /db_xref="CDD:214595"
     Region          59..72
                     /region_name="G1 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03113.4)"
     Region          200..208
                     /region_name="G2 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03113.4)"
     Site            208
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q14344; propagated from
                     UniProtKB/Swiss-Prot (Q03113.4)"
     Region          223..232
                     /region_name="G3 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03113.4)"
     Region          292..299
                     /region_name="G4 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03113.4)"
     Region          351..356
                     /region_name="G5 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03113.4)"
     CDS             1..381
                     /gene="GNA12"
                     /gene_synonym="gep; HG1M1; NNX3; RMP"
                     /coded_by="NM_007353.3:148..1293"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS5335.1"
                     /db_xref="GeneID:2768"
                     /db_xref="HGNC:HGNC:4380"
                     /db_xref="MIM:604394"
ORIGIN      
        1 msgvvrtlsr cllpaeagga rerragsgar daerearrrs rdidallare rravrrlvki
       61 lllgagesgk stflkqmrii hgrefdqkal lefrdtifdn ilkgsrvlvd ardklgipwq
      121 ysenekhgmf lmafenkagl pvepatfqly vpalsalwrd sgireafsrr sefqlgesvk
      181 yfldnldrig qlnyfpskqd illarkatkg ivehdfvikk ipfkmvdvgg qrsqrqkwfq
      241 cfdgitsilf mvssseydqv lmedrrtnrl vesmnifeti vnnklffnvs iilflnkmdl
      301 lvekvktvsi kkhfpdfrgd phrledvqry lvqcfdrkrr nrskplfhhf ttaidtenvr
      361 fvfhavkdti lqenlkdiml q
//
