LOCUS       NM_001002002            1595 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens guanosine monophosphate reductase 2 (GMPR2),
            transcript variant 4, mRNA.
ACCESSION   NM_001002002
VERSION     NM_001002002.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Baker,B.G., Ball,G.R., Rakha,E.A., Nolan,C.C., Caldas,C.,
            Ellis,I.O. and Green,A.R.
  TITLE     Lack of expression of the proteins GMPR2 and PPARalpha are
            associated with the basal phenotype and patient outcome in breast
            cancer
  JOURNAL   Breast Cancer Res Treat 137 (1), 127-137 (2013)
   PUBMED   23208589
  REMARK    GeneRIF: Loss of expression of GMPR2 and PPARalpha is associated
            with breast cancer basal phenotype; indicating that they may play a
            role in carcinogenesis.
REFERENCE   3  (bases 1 to 1595)
  AUTHORS   Patton,G.C., Stenmark,P., Gollapalli,D.R., Sevastik,R., Kursula,P.,
            Flodin,S., Schuler,H., Swales,C.T., Eklund,H., Himo,F., Nordlund,P.
            and Hedstrom,L.
  TITLE     Cofactor mobility determines reaction outcome in the IMPDH and GMPR
            (beta-alpha)8 barrel enzymes
  JOURNAL   Nat Chem Biol 7 (12), 950-958 (2011)
   PUBMED   22037469
  REMARK    GeneRIF: A single crystal structure of human GMPR type 2 with IMP
            and NADPH fortuitously captures three different states, each of
            which mimics a distinct step in the catalytic cycle of guanosine
            monophosphate reductase(GMPR).
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1595)
  AUTHORS   Zhang,J., Zhang,W., Zou,D., Chen,G., Wan,T., Zhang,M. and Cao,X.
  TITLE     Cloning and functional characterization of GMPR2, a novel human
            guanosine monophosphate reductase, which promotes the monocytic
            differentiation of HL-60 leukemia cells
  JOURNAL   J Cancer Res Clin Oncol 129 (2), 76-83 (2003)
   PUBMED   12669231
  REMARK    GeneRIF: GMPR2 is a novel human GMP reductase, and overexpression
            of GMPR2 can promote the monocytic differentiation of HL-60
            leukemia cells.
REFERENCE   5  (bases 1 to 1595)
  AUTHORS   Deng,Y., Wang,Z., Ying,K., Gu,S., Ji,C., Huang,Y., Gu,X., Wang,Y.,
            Xu,Y., Li,Y., Xie,Y. and Mao,Y.
  TITLE     NADPH-dependent GMP reductase isoenzyme of human (GMPR2).
            Expression, purification, and kinetic properties
  JOURNAL   Int J Biochem Cell Biol 34 (9), 1035-1050 (2002)
   PUBMED   12009299
  REMARK    GeneRIF: Data suggest the existence of two distinct types of human
            GMP reductase molecular species, which can be used to explain the
            bimodal saturation curve noted with purified human erythrocyte GMP
            reductase.
REFERENCE   6  (bases 1 to 1595)
  AUTHORS   Spector,T., Jones,T.E. and Miller,R.L.
  TITLE     Reaction mechanism and specificity of human GMP reductase.
            Substrates, inhibitors, activators, and inactivators
  JOURNAL   J Biol Chem 254 (7), 2308-2315 (1979)
   PUBMED   218932
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP347823.1 and BC009832.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001002002.2.
            
            Summary: This gene encodes an enzyme that catalyzes the
            irreversible and NADPH-dependent reductive deamination of guanosine
            monophosphate (GMP) to inosine monophosphate (IMP). The protein
            also functions in the re-utilization of free intracellular bases
            and purine nucleosides. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (4) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream in-frame start codon, compared to variant 1. The encoded
            isoform (2) has a shorter N-terminus than isoform 1. Variants 2, 3,
            and 4 encode the same isoform (2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC009832.2, SRR5189655.115351.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399440.7/ ENSP00000382369.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                BP347823.1         58-82
            26-1595             BC009832.2         1-1570
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q12"
     gene            1..1595
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /note="guanosine monophosphate reductase 2"
                     /db_xref="GeneID:51292"
                     /db_xref="HGNC:HGNC:4377"
                     /db_xref="MIM:610781"
     exon            1..49
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            50..171
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1131
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /EC_number="1.7.1.7"
                     /note="isoform 2 is encoded by transcript variant 4;
                     guanosine monophosphate reductase isolog; GMP reductase 2;
                     guanosine 5'-monophosphate oxidoreductase 2"
                     /codon_start=1
                     /product="GMP reductase 2 isoform 2"
                     /protein_id="NP_001002002.1"
                     /db_xref="CCDS:CCDS41935.1"
                     /db_xref="GeneID:51292"
                     /db_xref="HGNC:HGNC:4377"
                     /db_xref="MIM:610781"
                     /translation="MPHIDNDVKLDFKDVLLRPKRSTLKSRSEVDLTRSFSFRNSKQT
                     YSGVPIIAANMDTVGTFEMAKVLCKFSLFTAVHKHYSLVQWQEFAGQNPDCLEHLAAS
                     SGTGSSDFEQLEQILEAIPQVKYICLDVANGYSEHFVEFVKDVRKRFPQHTIMAGNVV
                     TGEMVEELILSGADIIKVGIGPGSVCTTRKKTGVGYPQLSAVMECADAAHGLKGHIIS
                     DGGCSCPGDVAKAFGAGADFVMLGGMLAGHSESGGELIERDGKKYKLFYGMSSEMAMK
                     KYAGGVAEYRASEGKTVEVPFKGDVEHTIRDILGGIRSTCTYVGAAKLKELSRRTTFI
                     RVTQQVNPIFSEAC"
     exon            172..291
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            292..375
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            376..549
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            550..631
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            632..738
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            739..781
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..941
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1595
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1568..1573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /note="hexamer: AATAAA"
     polyA_site      1595
                     /gene="GMPR2"
                     /gene_synonym="GMPR 2"
                     /note="major polyA site"
ORIGIN      
        1 ctcttgcccc attgctcttt gcaggggtag aagaaggaag tgtagcgggc cctcagattc
       61 atcgctaccc cgaggctaag cgccatgcct catattgaca acgatgtgaa actggacttc
      121 aaggatgtcc ttttgaggcc caaacgcagt acccttaagt ctcgaagtga ggtggatctc
      181 acaagatcct tttcatttcg gaactcaaag cagacatact ctggggttcc catcattgct
      241 gccaatatgg atactgtggg cacctttgag atggccaagg ttctctgtaa gttctctctc
      301 ttcactgctg tccataagca ctatagcctc gttcagtggc aagagtttgc tggccagaat
      361 cctgactgtc ttgagcatct ggctgccagc tcaggcacag gctcttctga ctttgagcag
      421 ctggaacaga tcctggaagc tattccccag gtgaagtata tatgcctgga tgtggcaaat
      481 ggctactctg aacactttgt tgaatttgta aaagatgtac ggaagcgctt cccccagcac
      541 accatcatgg cagggaatgt ggtaacagga gagatggtag aagagctcat cctttctggg
      601 gctgacatca tcaaagtggg aattgggcca ggctctgtgt gtactactcg gaagaaaact
      661 ggagtggggt atccacagct cagcgcagtg atggagtgtg cagatgctgc tcatggcctc
      721 aaaggccaca tcatttcaga tggaggttgc agctgtcctg gggatgtggc caaggctttt
      781 ggggcaggag ctgacttcgt gatgctgggt ggcatgctgg ctgggcacag tgagtcaggt
      841 ggtgagctca tcgagaggga tggcaagaag tacaagctct tctatggaat gagttctgaa
      901 atggccatga agaagtatgc tgggggcgtg gctgagtaca gagcctcaga gggaaagaca
      961 gtggaagttc cttttaaagg agatgtggaa cataccatcc gagacatcct aggagggatc
     1021 cgctctacgt gtacctatgt gggagcagct aagctcaaag agttgagcag gagaactacc
     1081 ttcatccgag tcacccagca ggtgaatcca atcttcagtg aggcgtgcta gacctgagca
     1141 gttctaccct cccaaggcac cagtactcta ccatggggca tcccaagtgg ggtcctcacc
     1201 catcccagct actgcagctc tgtattactt tgtcatttcc tgttgtctca ctcctgaggg
     1261 ctcctgcagt aactctgtac ttctctatct gcacacacaa aatgcccaag gcactcactg
     1321 gggaggaagc aaggaagcaa acagtctgag aaaatgatgc aagaaaatca aatgggaatc
     1381 tggggaccca acacaacatc ctgaagatta ttaaaaggaa aagatgctga ttggtacata
     1441 aatcttttac atggccttgg tctagaggag gcaggctttt agaatcatgt tttgttaatc
     1501 cgcttcacta aattggacct tcacatatct aaaaagctct gaagtgtttg tatatttgaa
     1561 atacctcaat aaagagagag ctcattgact gtaaa
//
