LOCUS       NM_001033044            7554 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens glutamate-ammonia ligase (GLUL), transcript variant 2,
            mRNA.
ACCESSION   NM_001033044
VERSION     NM_001033044.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Wang,D., Song,T., Hu,Z., Wang,H. and Qian,J.
  TITLE     Role of Glutamine Synthetase on Vascular Permeability in Gliomas
  JOURNAL   Anticancer Res 44 (11), 4869-4875 (2024)
   PUBMED   39477285
  REMARK    GeneRIF: Role of Glutamine Synthetase on Vascular Permeability in
            Gliomas.
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Jones,A.G., Aquilino,M., Tinker,R.J., Duncan,L., Jenkins,Z.,
            Carvill,G.L., DeWard,S.J., Grange,D.K., Hajianpour,M.J.,
            Halliday,B.J., Holder-Espinasse,M., Horvath,J., Maitz,S., Nigro,V.,
            Morleo,M., Paul,V., Spencer,C., Esterhuizen,A.I., Polster,T.,
            Spano,A., Gomez-Lozano,I., Kumar,A., Poke,G., Phillips,J.A. 3rd,
            Underhill,H.R., Gimenez,G., Namba,T. and Robertson,S.P.
  TITLE     Clustered de novo start-loss variants in GLUL result in a
            developmental and epileptic encephalopathy via stabilization of
            glutamine synthetase
  JOURNAL   Am J Hum Genet 111 (4), 729-741 (2024)
   PUBMED   38579670
  REMARK    GeneRIF: Clustered de novo start-loss variants in GLUL result in a
            developmental and epileptic encephalopathy via stabilization of
            glutamine synthetase.
REFERENCE   3  (bases 1 to 7554)
  AUTHORS   Buccoliero,A.M., Caporalini,C., Moscardi,S., Cetica,V., Mei,D.,
            Conti,V., Nozzoli,F., Bonaudo,C., Battista,F., Giordano,F.,
            Mura,R., Spacca,B., Mussa,F., D'Onofrio,V., Guerrini,R.,
            Genitori,L. and Scagnet,M.
  TITLE     Leat-associated seizures the possible role of EAAT2, pyruvate
            carboxylase and glutamine synthetase
  JOURNAL   Epilepsy Res 199, 107258 (2024)
   PUBMED   38086219
  REMARK    GeneRIF: Leat-associated seizures the possible role of EAAT2,
            pyruvate carboxylase and glutamine synthetase.
REFERENCE   4  (bases 1 to 7554)
  AUTHORS   Jiang,J., Hu,Y., Fang,D. and Luo,J.
  TITLE     Glutamine synthetase and hepatocellular carcinoma
  JOURNAL   Clin Res Hepatol Gastroenterol 47 (10), 102248 (2023)
   PUBMED   37979911
  REMARK    GeneRIF: Glutamine synthetase and hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 7554)
  AUTHORS   Wang,Y., Kudoh,J., Kubota,R., Asakawa,S., Minoshima,S. and
            Shimizu,N.
  TITLE     Chromosomal mapping of a family of human glutamine synthetase
            genes: functional gene (GLUL) on 1q25, pseudogene (GLULP) on 9p13,
            and three related genes (GLULL1, GLULL2, GLULL3) on 5q33, 11p15,
            and 11q24
  JOURNAL   Genomics 37 (2), 195-199 (1996)
   PUBMED   8921392
REFERENCE   6  (bases 1 to 7554)
  AUTHORS   Schraml,P., Shipman,R., Colombi,M. and Ludwig,C.U.
  TITLE     Identification of genes differentially expressed in normal lung and
            non-small cell lung carcinoma tissue
  JOURNAL   Cancer Res 54 (19), 5236-5240 (1994)
   PUBMED   7923146
REFERENCE   7  (bases 1 to 7554)
  AUTHORS   Christa,L., Simon,M.T., Flinois,J.P., Gebhardt,R., Brechot,C. and
            Lasserre,C.
  TITLE     Overexpression of glutamine synthetase in human primary liver
            cancer
  JOURNAL   Gastroenterology 106 (5), 1312-1320 (1994)
   PUBMED   7909780
REFERENCE   8  (bases 1 to 7554)
  AUTHORS   Van den Hoff,M.J., Geerts,W.J., Das,A.T., Moorman,A.F. and
            Lamers,W.H.
  TITLE     cDNA sequence of the long mRNA for human glutamine synthase
  JOURNAL   Biochim Biophys Acta 1090 (2), 249-251 (1991)
   PUBMED   1681907
REFERENCE   9  (bases 1 to 7554)
  AUTHORS   Gibbs,C.S., Campbell,K.E. and Wilson,R.H.
  TITLE     Sequence of a human glutamine synthetase cDNA
  JOURNAL   Nucleic Acids Res 15 (15), 6293 (1987)
   PUBMED   2888076
REFERENCE   10 (bases 1 to 7554)
  AUTHORS   Rivett,A.J.
  TITLE     Preferential degradation of the oxidatively modified form of
            glutamine synthetase by intracellular mammalian proteases
  JOURNAL   J Biol Chem 260 (1), 300-305 (1985)
   PUBMED   2856920
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA151535.1, BC011852.2,
            AI147221.1, BM690132.1, AL139344.24, AW665656.1, DA322310.1,
            BQ637044.1, DB317032.1, AW954632.1, CD300750.1, BI668874.1,
            BF923451.1 and AI286093.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001033044.3.
            
            Summary: The protein encoded by this gene belongs to the glutamine
            synthetase family. It catalyzes the synthesis of glutamine from
            glutamate and ammonia in an ATP-dependent reaction. This protein
            plays a role in ammonia and glutamate detoxification, acid-base
            homeostasis, cell signaling, and cell proliferation. Glutamine is
            an abundant amino acid, and is important to the biosynthesis of
            several amino acids, pyrimidines, and purines. Mutations in this
            gene are associated with congenital glutamine deficiency, and
            overexpression of this gene was observed in some primary liver
            cancer samples. There are six pseudogenes of this gene found on
            chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (2) lacks an exon in the 5' UTR
            compared to variant 1. Both variants 1, 2 and 3 encode the same
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX537384.1, SRR11853563.2472.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331872.11/ ENSP00000356537.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-496               DA151535.1         62-557
            497-1979            BC011852.2         492-1974
            1980-2756           BC011852.2         1976-2752
            2757-2887           AI147221.1         5-135               c
            2888-3156           BM690132.1         12-280
            3157-3385           AL139344.24        38909-39137         c
            3386-3789           AW665656.1         172-575             c
            3790-4041           DA322310.1         81-332
            4042-4042           AL139344.24        38252-38252         c
            4043-4292           DA322310.1         334-583
            4293-4839           AL139344.24        37455-38001         c
            4840-5428           BQ637044.1         46-634
            5429-5790           DB317032.1         1-362               c
            5791-6344           AW954632.1         153-706
            6345-6345           CD300750.1         17-17
            6346-6366           AW954632.1         708-728
            6367-6874           BI668874.1         20-527
            6875-7024           CD300750.1         547-696
            7025-7391           BF923451.1         34-400              c
            7392-7554           AI286093.1         12-174              c
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.3"
     gene            1..7554
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="glutamate-ammonia ligase"
                     /db_xref="GeneID:2752"
                     /db_xref="HGNC:HGNC:4341"
                     /db_xref="MIM:138290"
     exon            1..112
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     exon            113..291
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1247
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /EC_number="6.3.1.2"
                     /EC_number="2.3.1.225"
                     /note="glutamine synthase; proliferation-inducing protein
                     43; palmitoyltransferase GLUL; cell proliferation-inducing
                     protein 59; glutamate decarboxylase"
                     /codon_start=1
                     /product="glutamine synthetase"
                     /protein_id="NP_001028216.1"
                     /db_xref="CCDS:CCDS1344.1"
                     /db_xref="GeneID:2752"
                     /db_xref="HGNC:HGNC:4341"
                     /db_xref="MIM:138290"
                     /translation="MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKT
                     RTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVF
                     KYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGP
                     YYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHL
                     WVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEK
                     LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKK
                     GYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN"
     exon            292..453
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     exon            454..600
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     exon            601..728
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     exon            729..928
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     exon            929..7554
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2737..2742
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="hexamer: TATAAA"
     polyA_site      2758
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="major polyA site"
     regulatory      2801..2806
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="hexamer: ATTATA"
     polyA_site      2842
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
     polyA_site      3854
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
     regulatory      3923..3928
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="hexamer: ACTAAA"
     polyA_site      3948
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
     regulatory      4196..4201
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="hexamer: AATAAT"
     polyA_site      4215
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
     regulatory      6417..6422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="hexamer: AACAAA"
     polyA_site      6434
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
     regulatory      7536..7541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
                     /note="hexamer: AATAAA"
     polyA_site      7554
                     /gene="GLUL"
                     /gene_synonym="DEE116; GLNS; GS; PIG43; PIG59"
ORIGIN      
        1 agagtcgaga gtgggagaag agcggagcgt gtgagcagta ctgcggcctc ctctcctctc
       61 ctaacctcgc tctcgcggcc tagctttacc cgcccgcctg ctcggcgacc agaacacctt
      121 ccaccatgac cacctcagca agttcccact taaataaagg catcaagcag gtgtacatgt
      181 ccctgcctca gggtgagaaa gtccaggcca tgtatatctg gatcgatggt actggagaag
      241 gactgcgctg caagacccgg accctggaca gtgagcccaa gtgtgtggaa gagttgcctg
      301 agtggaattt cgatggctct agtactttac agtctgaggg ttccaacagt gacatgtatc
      361 tcgtgcctgc tgccatgttt cgggacccct tccgtaagga ccctaacaag ctggtgttat
      421 gtgaagtttt caagtacaat cgaaggcctg cagagaccaa tttgaggcac acctgtaaac
      481 ggataatgga catggtgagc aaccagcacc cctggtttgg catggagcag gagtataccc
      541 tcatggggac agatgggcac ccctttggtt ggccttccaa cggcttccca gggccccagg
      601 gtccatatta ctgtggtgtg ggagcagaca gagcctatgg cagggacatc gtggaggccc
      661 attaccgggc ctgcttgtat gctggagtca agattgcggg gactaatgcc gaggtcatgc
      721 ctgcccagtg ggaatttcag attggacctt gtgaaggaat cagcatggga gatcatctct
      781 gggtggcccg tttcatcttg catcgtgtgt gtgaagactt tggagtgata gcaacctttg
      841 atcctaagcc cattcctggg aactggaatg gtgcaggctg ccataccaac ttcagcacca
      901 aggccatgcg ggaggagaat ggtctgaagt acatcgagga ggccattgag aaactaagca
      961 agcggcacca gtaccacatc cgtgcctatg atcccaaggg aggcctggac aatgcccgac
     1021 gtctaactgg attccatgaa acctccaaca tcaacgactt ttctgctggt gtagccaatc
     1081 gtagcgccag catacgcatt ccccggactg ttggccagga gaagaagggt tactttgaag
     1141 atcgtcgccc ctctgccaac tgcgacccct tttcggtgac agaagccctc atccgcacgt
     1201 gtcttctcaa tgaaaccggc gatgagccct tccagtacaa aaattaagtg gactagacct
     1261 ccagctgttg agcccctcct agttcttcat cccactccaa ctcttccccc tctcccagtt
     1321 gtcccgattg taactcaaag ggtggaatat caaggtcgtt tttttcattc catgtgccca
     1381 gttaatcttg ctttctttgt ttggctggga tagaggggtc aagttattaa tttcttcaca
     1441 cctaccctcc tttttttccc tatcactgaa gctttttagt gcattagtgg ggaggagggt
     1501 ggggagacat aaccactgct tccatttaat ggggtgcacc tgtccaatag gcgtagctat
     1561 ccggacagag cacgtttgca gaagggggtc tcttcttcca ggtagctgaa aggggaagac
     1621 ctgacgtact ctggttaggt taggacttgc cctcgtggtg gaaacttttc ttaaaaagtt
     1681 ataaccaact tttctattaa aagtgggaat taggagagaa ggtaggggtt gggaatcaga
     1741 gagaatggct ttggtctctt gcttgtggga ctagcctggc ttgggactaa atgccctgct
     1801 ctgaacacga agcttagtat aaactgatgg atatccctac cttgaaagaa gaaaaggttc
     1861 ttactgcttg gtccttgatt tatcacacaa agcagaatag tatttttata tttaaatgta
     1921 aagacaaaaa actatatgta tggttttgtg gattatgtgt gttttgctaa aggaaaaaac
     1981 catccaggtc acggggcacc aaatttgaga caaatagtcg gattagaaat aaagcatctc
     2041 attttgagta gagagcaagg gaagtggttc ttagatggtg atctgggatt aggccctcaa
     2101 gacccttttg ggtttctgcc ctgcccaccc tctggagaag gtgggcactg gattagttaa
     2161 cagacaacac gttactagca gtcacttgat ctccgtggct ttggtttaaa agacacactt
     2221 gtccacatag gtttagagat aagagttggc tggtcaactt gagcatgtta ctgacagagg
     2281 gggtattggg gttattttct ggtaggaata gcatgtcact aaagcaggcc ttttgatatt
     2341 aaatttttta aaaagcaaaa ttatagaagt ttagatttta atcaaatttg tagggtttct
     2401 aggtaatttt tacagaattg cttgtttgct tcaactgtct cctacctctg ctcttggagg
     2461 agatggggac agggctggag tcaaaacact tgtaattttg tatcttgatg tctttgttaa
     2521 gactgctgaa gaattatttt ttttctttta taataaggaa taaaccccac ctttattcct
     2581 tcatttcatc taccattttc tggttcttgt gttggctgtg gcaggccagc tgtggttttc
     2641 ttttgccatg acaacttcta attgccatgt acagtatgtt caaagtcaaa taactcctca
     2701 ttgtaaacaa actgtgtaac tgcccaaagc agcacttata aatcagccta acataagatc
     2761 tctctgatgt gtttgtgatt ctttcaaatc cctatgtgcc attatatttc tttatttcct
     2821 aaaacaggca aaataagctc aagtttatgt actctgagtt tttaaaacac tggagtgatg
     2881 ttgctgacca gccgtttcct gtacctctct aagttgggta tttgggactt aagggattaa
     2941 gtttttcacc tagacttagt tacacacaat cttggcattt cctagcctag aggtttgtag
     3001 cagggtacaa gccccactcc tcccccttcc tttgctcccc tgagtttggt tttggcttac
     3061 cataacattg ttttgaccat tcctagccta atacaatagc ctaacataat gtaagattaa
     3121 ctggctttac gatttctatt ctctgctctc agtgataaga aacaaatatt agctaccctg
     3181 ctaccctggt tgaagccttc caaggctggc tatgccctag gcatgggctc atccttgggt
     3241 gtatcttgcc ttgcaggaag accagtggac cgattgtgat tctcaaaagc tctgtgttgt
     3301 cacctgtgcc cttgcccctt gctcttatct tggtccgtgt atctgggagt tcttccacct
     3361 tatcttggcc aattcctacc ttcgttcatt cctcatgagg ttgggtaaaa gctccctccg
     3421 gctcccatga tgctgtgcat atacctagca aaaagcaatt attggacaca ttggagtgca
     3481 atattattaa tagcattaat actactaata atgtgggcaa tagtgattgt ttttaaaagg
     3541 cagtatactc ttaccagtgc gaggtagctg gggcctgtga tagtttttag agataagttc
     3601 ttcaggcaac tgtgtatttt acactagtca agtaatccta gatatccgtg gtttttctta
     3661 agaaagttgg ctcgtaatat gatttaatat tcaaagtaga gtcatctacc tattagcttg
     3721 ctggcgtggt cctagtttat gcctgtttca gcatgattgt tgagtaccct gtttcatcct
     3781 tagcattttc ttgattttgt tgttaaatga tgtataccct tatttccatt gaatctgtgc
     3841 ttccaccccc ccaactgaag ttgtcttccc tttgcttggc cacccttaca gcctcttgga
     3901 tggtgtatcc tacagtgtaa gcactaaact gaagaggcag tgacctgagc actttggatt
     3961 ttgttcattg taatcaattc catgacaaaa tgattgcatg agaaggaatt ttaaattcat
     4021 aggatcagaa tttaggtgaa aacaaccagc atatttgttt cttcaccctc tcacctagaa
     4081 ttagctttga cctacaggtc acagtgcaat ccccttgtat ttctaaggtg ttttttatag
     4141 ttcatttgca gacaatgggt tatgtgataa cttttatcag tgatagatta aacagaataa
     4201 tgaccaagct ttcaacctta aggagtcagg ccagtattta caaaaggagg tctccatgaa
     4261 ctccttaaat atgagttccc ctaatatcat cttgccaggt actaaataac aactgatagc
     4321 acaagctata gggaatttga aagaattcca tggatgggtg ttgtctaggg ccttttgttg
     4381 tttttgagac ggggtctgac tctcacccag gctggagtat agtgtggcgc aatcttggct
     4441 cactgcaact tctgcctccc agattcaagc gattctcctg cctcagcctc ccaagtagct
     4501 gagactacag gtgtgcacca ccatgctcag ctaatttttg tatttttagt acagatgggg
     4561 tttcaccatg ttggccaggc tggtcttgaa ctcctgatct cccaaagtga ggtcttgaac
     4621 tggtcttgaa ctcctccacc tcccaaagtg ctgggattac aggcgtgagc cactgcaccc
     4681 ggcctagggc catgtaaaaa gccagatctg tgctgctgtc tgtgtagaag ggtagacaag
     4741 tggatgagaa gttcctgaac tattcttggc ccttttacca ctaagtgaaa gtaacttgct
     4801 gccccaaaga aagatgtctc atcattcgac aggactttct agttgaactt catgaaagca
     4861 agagatcctg tttttcttgc tcaccactgt atcttgagac ctgttgtagt gcctgcaata
     4921 cttatttaat aagttatttt taagtatcag ttttgtgagc tttaactcta tgaggtcttt
     4981 gttgtttgac tgtattttaa ctctggccat gacagcaaga caaagttcca tttttattga
     5041 gcttaaaaag aatcaaggcc aggtgaagtg gcttacgcct gtgatcccaa cactttgtga
     5101 ggctgcagca ggaggatctc ttgagcccag gagtttgaga ccgttctagg caatgtagtg
     5161 aggtccagac tccacaaaat aatttttttt taaattgcac gcctgtagtc tcagctatca
     5221 ggaggctgag atgggaggat gacttgagcc caggaaattg aagctgcagt gaattgtgat
     5281 tgcaccactg cactccagcc tgggtgacag atcaagacct tgcctaaaca aaacaaaaca
     5341 aacaaaaccc caaaaaacaa attgaaaatg ttgattcttt ttactacaaa cattatggca
     5401 gcactaaaaa cttcgtggga gtgtactgtg gaaaatagtg tacttaatta attctcattg
     5461 taatcaggct accaagagcc ttgtgttgct ttaagagtta taactgccag gcacagtggc
     5521 tcatgcctat aatcccagca ccttgagagg ccgaggcagg tggatcacct gagatcggga
     5581 gtttgagacc agccgggcca atatggtgaa acaagctgtg tctctactaa atacaaaaaa
     5641 ttagccgggc gtggtggcac atgcctgtaa tcccagctgc ttgggagact gagacaggag
     5701 aattgcttga acctggaagg cggaggttgc agtgagctga gattgcaaca ttgtactcca
     5761 gcctgggcaa caagagggaa actccatctc aaaaaaaaaa aaaaagttgt aactgaggct
     5821 gggcatggtg gctcatacct gtaatcccag cactttgaaa agccgaggca ggtagatcac
     5881 ttgagctcag aagttcgaga ctagcctggg caacatgaca aaaccccatc tctacaaaaa
     5941 atacgaaaaa ttagctgggc gtggtggcat gcacctgtag tcctagctac ctgggaggct
     6001 gaggtgggaa gattacttga agctgcagtg agccatggtt gtgccactgc cctccagtct
     6061 gggcaacaaa gtgagaccct gtctcaaaaa aacaaaaaaa aattataact gatgtaaact
     6121 ggcagtttag gctgggtgtg gtggctcaag cctgtaatcc tagcactttg ggaggccaag
     6181 gcaggtggat cacctgagtt caggagttcg agaccagcgt ggccaacatg gtgaaacctt
     6241 gtctctatta aaaataccaa aattagcaag atgtggtggt gggtgcctat aattccagct
     6301 actcaggagg ctgaggcagg aggatcgctg gagccaggga ggcagaggtt acagtaagca
     6361 aagatcactc cacttcactc cagcctgggc aaaagagtga gacatatcaa aaaataaaca
     6421 aataaataaa taaataagtg gcagttcatc atttaactcc aaagactttg cgtacatttc
     6481 tactgaaaac aatctgagct gattagaacc ctgccatttt atagccttta gctcgatctc
     6541 cgaccgttca tttaaaaaaa ttctacttca ggccgggcat ggtggctcaa gcctgtaatc
     6601 ccatcactgt aggaggccaa agtgggcaga tcacttaagg tcaggagttt gagaccagcc
     6661 tggccaccat ggtgaaaccc catctctact aaaaatacaa aaattagccg ggcttggtgg
     6721 tgagcacctg taatcccacc ctgccgagtg gcaggctgag gcaggagaat cgcttgagcc
     6781 caagagccgg aggttgcagt gagccaagct tgcaccattg cactccagcc taggcaacag
     6841 agtgtgactc catctcaaga aaaaaaaaat tctatttcat tttacaatat gcagatatat
     6901 gtccatacac atgcataata taaatgtata ccatatttgt gagaatatgc atatatgtac
     6961 acattagata cacaatacaa gcacaataca tatgtctttt gcccaagata cagcattttg
     7021 taaaggagac aggaatttag taatatatgt tccagaaaca gtacacaaga gaattcgccg
     7081 agatgagaaa gttgtcacta ggaatgggga gtggtaagat gtagaaggta taattgttct
     7141 taaagttcta ctgccaactc tttccaatta attacccact ctgccatgct ttatggacag
     7201 gaggttgtcg gacactgtca attaataaat atttgagcat gatacactgc ttggagctcc
     7261 tctaatatag gagagtgata tcctagtgca tgttacagag ggagtgtcca cacagttcct
     7321 attgtcattt gatgagttac ttttcagggg ccttgtacct gagcaagttg tcctcttttt
     7381 gatggatttc agattgagtt acctgcattg tcttgagatt gcagcgtgtt tcctccactg
     7441 tacggcgtag tcagcagatc tattagttaa actccagtgg gccctcagtc actaaatcta
     7501 tcctctgtgt tgaaggcttt ctgcatttgc ctttcaataa aggtttagaa taac
//
