LOCUS       NM_014030               3783 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 2, mRNA.
ACCESSION   NM_014030
VERSION     NM_014030.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Wang,T., Su,K., Wang,L., Shi,Y., Niu,Y., Zhou,Y., Wang,A. and Wu,T.
  TITLE     Pan-cancer analysis of the oncogenic effects of G-protein-coupled
            receptor kinase-interacting protein-1 and validation on liver
            hepatocellular carcinoma
  JOURNAL   Adv Clin Exp Med 32 (10), 1139-1147 (2023)
   PUBMED   36994687
  REMARK    GeneRIF: Pan-cancer analysis of the oncogenic effects of
            G-protein-coupled receptor kinase-interacting protein-1 and
            validation on liver hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Zhang,S., Miyakawa,A., Wickstrom,M., Dyberg,C., Louhivuori,L.,
            Varas-Godoy,M., Kemppainen,K., Kanatani,S., Kaczynska,D.,
            Ellstrom,I.D., Elfman,L., Kronqvist,P., Repo,H., Mikoshiba,K.,
            Sahlgren,C., Johnsen,J.I. and Uhlen,P.
  TITLE     GIT1 protects against breast cancer growth through negative
            regulation of Notch
  JOURNAL   Nat Commun 13 (1), 1537 (2022)
   PUBMED   35318302
  REMARK    GeneRIF: GIT1 protects against breast cancer growth through
            negative regulation of Notch.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3783)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 3783)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3783)
  AUTHORS   Jiao,Y., Zhang,L., Li,J., He,Y., Zhang,X. and Li,J.
  TITLE     Exosomal miR-122-5p inhibits tumorigenicity of gastric cancer by
            downregulating GIT1
  JOURNAL   Int J Biol Markers 36 (1), 36-46 (2021)
   PUBMED   33752480
  REMARK    GeneRIF: Exosomal miR-122-5p inhibits tumorigenicity of gastric
            cancer by downregulating GIT1.
REFERENCE   6  (bases 1 to 3783)
  AUTHORS   Manabe,R., Kovalenko,M., Webb,D.J. and Horwitz,A.R.
  TITLE     GIT1 functions in a motile, multi-molecular signaling complex that
            regulates protrusive activity and cell migration
  JOURNAL   J Cell Sci 115 (Pt 7), 1497-1510 (2002)
   PUBMED   11896197
  REMARK    GeneRIF: GIT1 functions in a motile, multi-molecular signaling
            complex that regulates membrane protrusions and cell migration.
REFERENCE   7  (bases 1 to 3783)
  AUTHORS   Kawachi,H., Fujikawa,A., Maeda,N. and Noda,M.
  TITLE     Identification of GIT1/Cat-1 as a substrate molecule of protein
            tyrosine phosphatase zeta /beta by the yeast substrate-trapping
            system
  JOURNAL   Proc Natl Acad Sci U S A 98 (12), 6593-6598 (2001)
   PUBMED   11381105
REFERENCE   8  (bases 1 to 3783)
  AUTHORS   Zhao,Z.S., Manser,E., Loo,T.H. and Lim,L.
  TITLE     Coupling of PAK-interacting exchange factor PIX to GIT1 promotes
            focal complex disassembly
  JOURNAL   Mol Cell Biol 20 (17), 6354-6363 (2000)
   PUBMED   10938112
REFERENCE   9  (bases 1 to 3783)
  AUTHORS   Premont,R.T., Claing,A., Vitale,N., Perry,S.J. and Lefkowitz,R.J.
  TITLE     The GIT family of ADP-ribosylation factor GTPase-activating
            proteins. Functional diversity of GIT2 through alternative splicing
  JOURNAL   J Biol Chem 275 (29), 22373-22380 (2000)
   PUBMED   10896954
  REMARK    GeneRIF: Cloning of human GIT1 cDNA, and demonstration of GIT1
            interaction with PIX and paxillin proteins.
REFERENCE   10 (bases 1 to 3783)
  AUTHORS   Premont,R.T., Claing,A., Vitale,N., Freeman,J.L., Pitcher,J.A.,
            Patton,W.A., Moss,J., Vaughan,M. and Lefkowitz,R.J.
  TITLE     beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled
            receptor kinase-associated ADP ribosylation factor
            GTPase-activating protein
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14082-14087 (1998)
   PUBMED   9826657
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104564.11, CF994106.1, AF124490.1, DR001908.1, AK024826.1 and
            BM984210.1.
            
            On Jun 2, 2019 this sequence version replaced NM_014030.3.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon, compared to variant 1, resulting in a shorter protein
            (isoform 2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF124490.1, SRR7346977.1702777.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000225394.8/ ENSP00000225394.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                AC104564.11        131862-131917
            57-266              CF994106.1         63-272
            267-548             AF124490.1         11-292
            549-631             DR001908.1         474-556
            632-1202            AF124490.1         376-946
            1203-1799           AK024826.1         302-898
            1800-2686           AF124490.1         1544-2430
            2687-2738           AK024826.1         1783-1834
            2739-3128           AK024826.1         1836-2225
            3129-3216           AF124490.1         2876-2963
            3217-3783           BM984210.1         25-591              c
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..3783
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /note="GIT ArfGAP 1"
                     /db_xref="GeneID:28964"
                     /db_xref="HGNC:HGNC:4272"
                     /db_xref="MIM:608434"
     exon            1..322
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             271..2556
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /EC_number="3.6.5.2"
                     /note="isoform 2 is encoded by transcript variant 2; ARF
                     GTPase-activating protein GIT1; cool-associated and
                     tyrosine-phosphorylated protein 1; CAT1; CAT-1; ARF GAP
                     GIT1; G protein-coupled receptor kinase interacting ArfGAP
                     1; GRK-interacting protein 1; G protein-coupled receptor
                     kinase-interactor 1"
                     /codon_start=1
                     /product="ARF GTPase-activating protein GIT1 isoform 2"
                     /protein_id="NP_054749.2"
                     /db_xref="CCDS:CCDS11250.1"
                     /db_xref="GeneID:28964"
                     /db_xref="HGNC:HGNC:4272"
                     /db_xref="MIM:608434"
                     /translation="MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRH
                     ISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPI
                     KSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQAN
                     FFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAER
                     LVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLF
                     EELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLAR
                     FNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDT
                     DQEPLRSTGATRSNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSL
                     SDELRRLQREIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFS
                     MYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVP
                     FTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDF
                     HPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAV
                     TEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQC
                     AYDIAKAAKQLVTITTREKKQ"
     exon            323..456
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            457..569
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            570..675
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            676..893
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            894..988
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1031
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1080
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1153
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1251
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1363
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1497
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1650
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1881
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1935
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2022
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2096
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2279
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2280..2343
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..3783
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3765..3770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /note="hexamer: AATAAA"
     polyA_site      3783
                     /gene="GIT1"
                     /gene_synonym="p95-APP1"
                     /note="major polyA site"
ORIGIN      
        1 agaagcggcc gggagccgcg cagggggcgg agagaggagc cgcgagcggc ggcggggcgg
       61 cgcggggcgg agggcgcccg agggcgagcg ggcaggcggg cacggcgggt tccgggccag
      121 ccccggggcg gacggttggc cgggcgcgcg gagcctcgcc ggggcctcgc cggagccggg
      181 ccgggcagca ggggccgccg ccgccgcccg ggcccgccgc ccgcgccccc cacgctggcc
      241 cagagggctg cggccgcgtc gccgctgagg atgtcccgaa aggggccgcg agcggaggtg
      301 tgtgcggact gcagcgcccc ggaccctggc tgggcatcca tcagcagggg tgtgctggtg
      361 tgtgacgagt gctgcagcgt gcaccggagc ctgggacgcc acatctccat tgtcaagcac
      421 cttcgccaca gcgcctggcc tcccacgctg ctgcagatgg tgcacacgct tgccagcaac
      481 ggggccaact ccatctggga gcactccctg ctggaccccg cacaagtgca gagcggccgg
      541 cgtaaagcca acccccaaga caaagtccac cccatcaagt cagagttcat cagggccaag
      601 taccagatgc tggcatttgt gcacaagctt ccctgccggg acgatgatgg agtcaccgcc
      661 aaagacctca gcaagcaact acactcgagc gtgcggacag gcaacctgga gacatgtctg
      721 cgcctgctct ccctgggtgc ccaggccaac ttcttccacc cagagaaggg caccacacct
      781 ctgcacgtgg ctgccaaggc aggacagaca ctgcaggccg agctgcttgt agtgtatggg
      841 gctgaccctg gctcccctga tgttaatggc cgcacaccca ttgactatgc caggcaggcg
      901 gggcaccatg agctggcgga aaggctggtt gagtgccaat atgagctcac tgaccggctg
      961 gccttctacc tctgtggacg caagccggat cacaagaatg ggcattacat catcccacag
     1021 atggctgaca gccttgactt atccgaattg gccaaagctg ctaagaagaa gctgcaggcg
     1081 ctcagcaacc ggctttttga ggaactcgcc atggacgtgt atgacgaggt ggatcgaaga
     1141 gaaaatgatg cagtgtggct ggctacccaa aaccacagca ctctggtgac agagcgcagt
     1201 gccgtgccct tcctgcctgt taacccggaa tactcagcca cgcggaatca ggggcgacaa
     1261 aagctggccc gctttaatgc ccgagagttt gccaccttga tcatcgacat tctcagtgag
     1321 gccaagcgga gacagcaggg caagagcctg agcagcccca cagacaacct cgagctgtct
     1381 ctgcggagcc agagtgacct cgacgaccaa cacgactacg acagcgtggc ctctgacgag
     1441 gacacagacc aggagcccct gcgcagcacc ggcgccactc ggagcaaccg ggcccggagc
     1501 atggactcct cggacttgtc tgacggggct gtgacgctgc aggagtacct ggagctgaag
     1561 aaggccctgg ctacatcgga ggcaaaggtg cagcagctca tgaaggtcaa cagtagcctg
     1621 agcgacgagc tccggaggct gcagcgagag atccacaagc tgcaggcgga gaacctgcag
     1681 ctccggcagc ctccagggcc ggtgcccaca cctccactcc ccagtgaacg ggcggaacac
     1741 acacccatgg cgccaggcgg gagcacacac cgcagggatc gccaggcctt ttccatgtat
     1801 gaacctggct ctgccctgaa gccctttggg ggcccccctg gggacgagct cactacgcgg
     1861 ctgcagcctt tccacagcac tgagctagag gacgacgcca tctattcagt gcacgtccct
     1921 gctggccttt accggatccg gaaaggggtg tctgcctcag ctgtgccctt cactccctcc
     1981 tccccgctgc tgtcctgctc ccaggaggga agccgccaca cgagcaagct ttcccgccac
     2041 ggcagtggag ccgacagtga ctatgagaac acgcaaagtg gggacccact gctggggctg
     2101 gaagggaaga ggtttctaga gctgggcaaa gaggaagact tccacccaga gctggaaagc
     2161 ctggatggag acctagatcc tgggcttccc agcacagagg atgtcatctt gaagacagag
     2221 caggtcacca agaacattca ggaactgttg cgggcagccc aggagttcaa gcatgacagc
     2281 ttcgtgccct gctcagagaa gatccatttg gctgtgaccg agatggcctc cctcttccca
     2341 aagaggccag ccctggagcc agtgcggagc tcactgcggc tgctcaacgc cagcgcctac
     2401 cggctgcaga gtgagtgccg gaagacagtg cccccagagc ccggcgcccc agtggacttc
     2461 cagctgctga ctcagcaggt gatccagtgc gcctatgaca tcgccaaggc tgccaagcag
     2521 ctggtcacca tcaccacccg agagaagaag cagtgacctc tctccccaca ccctcacctg
     2581 caccctagga cctcactggc cataggagct gggccactcc agacattaat ccccacccca
     2641 acagagccac tggcacaagt gcccttagtg ctgccacact ccctggcagc caggtgccct
     2701 ggtgcccacc cctgtcgagc ccctaaggat ggggaggtgg gggggcagga gcttctgtcc
     2761 cccacattcc atgcacctcc cctctgtata tagcatctcc cccctcctag tgagcagggg
     2821 cctgcaaggc atcactccca gcccctcgcc ttctagggca ccctcagcaa aggggcaggt
     2881 ggggacactc caagtggggc agctctccgt acatgcgccc cacccccatg agccagttca
     2941 gccctactgg gggctgagcg ggggcatccc ctcctttgta catagtctcc atggatgtcc
     3001 ctgccctgta gccaccagcc ccttgctgct ctccctttaa tgccatatgg cccctgccta
     3061 gggcacaggc cccaacctgt gtgctggggt ccccagcagc aaacactgga aagtctgttt
     3121 tttttttttc tttcttcttc cccacccctt aattttaact ttgtggtaac tgagtgcccc
     3181 cgcgtgcctg cgtgttgagt gtgtgggcgg cagtgccgtt ccggaggcct ggtccatctg
     3241 gagttttgag gggtgagggg accagagcag tgggaccagc atggggatca gcttcccttc
     3301 cccacctgga gccagggact gtccgggtag ccagttttgg tcctgccagc tgcctccctg
     3361 atccctcccc actctcgccc cttctctatg aacttaaatc aaaaaccact tccctccatc
     3421 tcctcctgct cctgcgtgga gggggaatgt gtgctggcta gggtggagga ctgagcacct
     3481 gagcctgggg ctggctcccc ggggtccccg actcagctgg tggctgtgga gctgagtccc
     3541 ctccccgtaa cctctgcaag gccagcaccc accatcacta cctgcacctg ctgtggtccc
     3601 accctctgga ggcctgggaa cctggctgca gcctgggaag gctggagagg cagacggtgg
     3661 gacccaccag ctctctcccc atcccgcttc ttccctgggg gccaggccct acctgtgtgg
     3721 tggtgggtgg gctgtcaaga cgtgtcatgt acatttgtat caaaaataaa gaagtgacca
     3781 tga
//
