LOCUS       NM_032336               3770 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens GINS complex subunit 4 (GINS4), mRNA.
ACCESSION   NM_032336
VERSION     NM_032336.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Zhou,Y., Yan,J., Chen,H., Zhou,W. and Yang,J.
  TITLE     MicroRNA-133a-3p Inhibits Lung Adenocarcinoma Development and
            Cisplatin Resistance through Targeting GINS4
  JOURNAL   Cells Tissues Organs 213 (1), 55-66 (2024)
   PUBMED   36273455
  REMARK    GeneRIF: MicroRNA-133a-3p Inhibits Lung Adenocarcinoma Development
            and Cisplatin Resistance through Targeting GINS4.
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Tomioka,Y., Suetsugu,T., Seki,N., Tanigawa,K., Hagihara,Y.,
            Shinmura,M., Asai,S., Kikkawa,N., Inoue,H. and Mizuno,K.
  TITLE     The Molecular Pathogenesis of Tumor-Suppressive miR-486-5p and
            miR-486-3p Target Genes: GINS4 Facilitates Aggressiveness in Lung
            Adenocarcinoma
  JOURNAL   Cells 12 (14), 1885 (2023)
   PUBMED   37508549
  REMARK    GeneRIF: The Molecular Pathogenesis of Tumor-Suppressive miR-486-5p
            and miR-486-3p Target Genes: GINS4 Facilitates Aggressiveness in
            Lung Adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3770)
  AUTHORS   Sun,L., Chen,S., Wang,T. and Bi,S.
  TITLE     Hsa_circ_0008673 Promotes Breast Cancer Progression by
            MiR-578/GINS4 Axis
  JOURNAL   Clin Breast Cancer 23 (3), 281-290 (2023)
   PUBMED   36628810
  REMARK    GeneRIF: Hsa_circ_0008673 Promotes Breast Cancer Progression by
            MiR-578/GINS4 Axis.
REFERENCE   4  (bases 1 to 3770)
  AUTHORS   Conte,M.I., Poli,M.C., Taglialatela,A., Leuzzi,G., Chinn,I.K.,
            Salinas,S.A., Rey-Jurado,E., Olivares,N., Veramendi-Espinoza,L.,
            Ciccia,A., Lupski,J.R., Aldave Becerra,J.C., Mace,E.M. and
            Orange,J.S.
  TITLE     Partial loss-of-function mutations in GINS4 lead to NK cell
            deficiency with neutropenia
  JOURNAL   JCI Insight 7 (21), e154948 (2022)
   PUBMED   36345943
  REMARK    GeneRIF: Partial loss-of-function mutations in GINS4 lead to NK
            cell deficiency with neutropenia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3770)
  AUTHORS   Zhang,K., Zhou,J., Wu,T., Tian,Q., Liu,T., Wang,W., Zhong,H.,
            Chen,Z., Xiao,X. and Wu,G.
  TITLE     Combined analysis of expression, prognosis and immune infiltration
            of GINS family genes in human sarcoma
  JOURNAL   Aging (Albany NY) 14 (14), 5895-5907 (2022)
   PUBMED   35896011
  REMARK    GeneRIF: Combined analysis of expression, prognosis and immune
            infiltration of GINS family genes in human sarcoma.
REFERENCE   6  (bases 1 to 3770)
  AUTHORS   Im,J.S., Ki,S.H., Farina,A., Jung,D.S., Hurwitz,J. and Lee,J.K.
  TITLE     Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires
            the Ctf4/And-1, RecQL4, and Mcm10 proteins
  JOURNAL   Proc Natl Acad Sci U S A 106 (37), 15628-15632 (2009)
   PUBMED   19805216
REFERENCE   7  (bases 1 to 3770)
  AUTHORS   Chang,Y.P., Wang,G., Bermudez,V., Hurwitz,J. and Chen,X.S.
  TITLE     Crystal structure of the GINS complex and functional insights into
            its role in DNA replication
  JOURNAL   Proc Natl Acad Sci U S A 104 (31), 12685-12690 (2007)
   PUBMED   17652513
REFERENCE   8  (bases 1 to 3770)
  AUTHORS   Labib,K. and Gambus,A.
  TITLE     A key role for the GINS complex at DNA replication forks
  JOURNAL   Trends Cell Biol 17 (6), 271-278 (2007)
   PUBMED   17467990
  REMARK    Review article
REFERENCE   9  (bases 1 to 3770)
  AUTHORS   Kamada,K., Kubota,Y., Arata,T., Shindo,Y. and Hanaoka,F.
  TITLE     Structure of the human GINS complex and its assembly and functional
            interface in replication initiation
  JOURNAL   Nat Struct Mol Biol 14 (5), 388-396 (2007)
   PUBMED   17417653
  REMARK    GeneRIF: The C-terminal domains of the Sld5 and Psf1 subunits are
            connected by linker regions to the core complex, and the C-terminal
            domain of Sld5 is important for core complex assembly.
REFERENCE   10 (bases 1 to 3770)
  AUTHORS   De Falco,M., Ferrari,E., De Felice,M., Rossi,M., Hubscher,U. and
            Pisani,F.M.
  TITLE     The human GINS complex binds to and specifically stimulates human
            DNA polymerase alpha-primase
  JOURNAL   EMBO Rep 8 (1), 99-103 (2007)
   PUBMED   17170760
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC005995.1 and AC009630.14.
            
            On Nov 23, 2018 this sequence version replaced NM_032336.2.
            
            Summary: The yeast heterotetrameric GINS complex is made up of
            Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3
            (GINS3; MIM 610610). The formation of the GINS complex is essential
            for the initiation of DNA replication in yeast and Xenopus egg
            extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for
            additional information about the GINS complex.[supplied by OMIM,
            Mar 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK313373.1, AK095334.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000276533.4/ ENSP00000276533.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-860               BC005995.1         35-894
            861-3770            AC009630.14        103595-106504       c
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.21"
     gene            1..3770
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /note="GINS complex subunit 4"
                     /db_xref="GeneID:84296"
                     /db_xref="HGNC:HGNC:28226"
                     /db_xref="MIM:610611"
     exon            1..136
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            137..251
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..827
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /note="SLD5 homolog"
                     /codon_start=1
                     /product="DNA replication complex GINS protein SLD5"
                     /protein_id="NP_115712.1"
                     /db_xref="CCDS:CCDS6116.1"
                     /db_xref="GeneID:84296"
                     /db_xref="HGNC:HGNC:28226"
                     /db_xref="MIM:610611"
                     /translation="MTEEVDFLGQDSDGGSEEVVLTPAELIERLEQAWMNEKFAPELL
                     ESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKIEK
                     FFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESYLKNVALKHMPPNLQKVDLFR
                     AVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYVIDLEKGSQHLIRYKTIAPLVASG
                     AVQLI"
     exon            252..338
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            339..452
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            453..550
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            551..639
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            640..730
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            731..3770
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3433
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /note="major polyA site"
     regulatory      3745..3750
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /note="hexamer: AATAAA"
     polyA_site      3770
                     /gene="GINS4"
                     /gene_synonym="SLD5"
                     /note="major polyA site"
ORIGIN      
        1 gagttggtcc cggcaagtcc ttgagcagtt tgttcctctg tcttcccgct tcctggtgcc
       61 ccgactgcgt ccccacgaac gccccgtcct taccgccggc tgctgtggag tgcctcgctg
      121 tctcccaggt ccccgagttc ctggtttcag agaagatgac cgaagaagtg gatttcctgg
      181 gacaggactc tgatgggggt agtgaggaag tggtcctaac tcctgcagag ctcattgaaa
      241 gattggagca ggcctggatg aatgaaaagt ttgcccctga gctgctggag agcaagcctg
      301 agattgtaga atgtgtcatg gaacagctgg agcacatgga agaaaatctc aggagagcca
      361 aaagggagga cctgaaggtc agcatccacc aaatggagat ggagaggatc cgctacgtcc
      421 tcagcagcta cttgcggtgt cgcctcatga agatagagaa gtttttccct catgtccttg
      481 agaaggaaaa aacacgtcct gagggggagc cttccagcct ctcgccggaa gagttggcct
      541 ttgccagaga gttcatggcg aacacagagt cctatctgaa aaatgtcgcc ttgaagcaca
      601 tgccccctaa cttacagaag gtggacctct ttcgggcagt tcccaaacca gatctagatt
      661 cttacgtgtt tctgagagtg agagaacgac aagaaaacat actggtagaa ccagacacag
      721 atgagcagag ggactacgtg attgacctgg agaagggctc acagcacttg atccgataca
      781 aaaccattgc acctctggtt gcatctggag ctgtccagct aatttaaaac taggcataaa
      841 cagccaggca tggtgactca agcctgtaat cccagcactt tgggaggccg aggcgggcgg
      901 atcatgaggt caggagttcg agaccaaccg accaacatgg tgaaacccca tctttactaa
      961 aaatacaaaa taattagccg ggtgttggtg gtgtgcacct gtaatcccag ctactcagga
     1021 ggccggggca ggagaatcgc ttgaacctgg gagcaggagg ttgcagtgag ccgaggtcgt
     1081 gccattgcac tccagcctgg gtgacagtga gactttgtct caaaaaaaaa aacaaaaaac
     1141 aaaaaaaaaa caaactaggc ataaactcat gaggtcagga gatcaagacc atcctggcta
     1201 ctaaacccca tctctactaa acccatctct actaaaaata caaaaaatta gccaggcatg
     1261 gtggcgggcc catctactca ggaggctgag gcaggagaat ggtgtgaacc cgggaggtgg
     1321 agcttgcagt gagccaagat cgtgccattg cactctagcc tgggcgacag agcgagactc
     1381 catttccaaa aaaaacacac taggcgtaaa caagaatgaa gacctggaac cgtagagaaa
     1441 atactagaaa cccctcgtat cacgttgtgc ctgaggacaa taataaagta gtttactagg
     1501 catcagacat tcctcagcag tgctgtgggt cacgggcgtg aagcggagct cgaggcagga
     1561 ttgcttgtgt tgctgcatgt cctgtctgct gttttagccc gtgtcagtag atggggccat
     1621 tgtactgttt gttctgcaca cttggcggag cagcagaggc tctcagcacg ccttttccct
     1681 ttgtagtatt ttctgagcta aggaaagtca ggcagaagta gttcataaga aggcatcact
     1741 gcctgtgatc ggggctgcat ttagccctga cctgataatg gtaggagagt tcatttagcc
     1801 tttctgctgc gaagattgta aatagattaa agaaaacagc tgtggccttc tcccgtcata
     1861 catcattaga ctgtgtaaac taacatttta gaacctctaa tattggactt aaaaccaatt
     1921 tctgtcgtgc ttaattgaaa atcacaacta aaccaataaa ccattattaa atttccattc
     1981 tgcagcagaa ggcacctctg gacggctccc ggcttgtagt ttaatgaagt atgtgctccc
     2041 tgctgttaac ccgcagccct ccccaactct ccagcagctt tgccaagtca gcctccgggc
     2101 catctggctg tccttggcca aggcactgtt aacggagtga cgttaaccat cttgtattgt
     2161 gacctgtggc cgcttttctg gtttaagtcc ttcacttttt caagaggaat ggatgaaatt
     2221 aatgtgcatg cagagtctca cagtgatgcc ggaaggaagg atgtataggc agtgaatgaa
     2281 tgatctttcc ttgaaatgct ataaaaagca acctgcattc cagtcttttc ttcctggttg
     2341 aagttggcag atttccattt gcagggtatg ggctgcgaga gtaatcaggg taacattgct
     2401 gtcatttcca agagaaatca gtaggggaaa gcaagggagg tctttttttt ttttcttttt
     2461 tgagacggag tttccctctt gttacccagg ctggagtggc agtggtgtga acacggctca
     2521 ctgcagcctc cacctcctgg gttcaagaga ttctcctgcc ccagcctccc gagtagctgg
     2581 tattataggt gcacaccacc acacccaact aattttttgt atttttagta gagatggggt
     2641 ttcatgatgt tggccaagct ggtctcgagc tcctgacccc aggtgatcca cccacctcgg
     2701 cctcccaaag tgctggaatt ataggcgtga gccactgcgc acggcctggg gaggttttat
     2761 ttcttgacaa aggtatttga tactcgtgca gtccctggag ggtctcactg gagagacaac
     2821 atttaggctg agatctgatt aacaggaggc agctgcagtg cagaggtcaa aagggagggt
     2881 gttccaggca gagaaaacag cctgtgcaaa ggccctgagg cagaaacaaa ctctacttga
     2941 ggtcagcctg gttagaaagc ccaactcaaa atagaaagta ttacatgata aggtctgagg
     3001 caggctggac ccagatctta caggaccttg ttaataagga tcccatttgg tcccccacag
     3061 tcctgagaag cgggcagggc tgtgggaaac agcagatatt tagtggtaag cctgagatca
     3121 gaacccaagt ctgcacttcc tagtcacgtt ctccctgtag tgctaagccc agagacctga
     3181 gctgttaacc tagaacagtg tgcttcctaa gccttaatgt gcatacccat cgcctggagc
     3241 tcgccttaag atgtaggttc tgcctgaagc ccaagttcat ttagtatgtc atggttaatt
     3301 cagagtaaaa tcaagagtta gtacttgatt tatgcttgtt atataaagaa agagacaact
     3361 tcactgtatg atcattttgt cacttttcaa aagcatttaa ttcccattca attgaaaatg
     3421 tttcaagaac aaacctgttt ggtcatttta ttgatattgc acatttgtat atgaataaat
     3481 ttttgcaaat taaaaaaaaa aaagatgtag gttctgaaac agggtgaggt ccaggatcct
     3541 gcctttctaa ggagctccca tgttgtgcag ttgctgctgg cccggggacc acattgaatg
     3601 acggctctac gtcctcatgc ctccagctgc tgccctgtac atgtccaact gcacggagca
     3661 ctttacatcc tctaaaacca cagtaaattg ctccatttcc taaattacct cttcaagaga
     3721 agactggttt tgatgtcatt tttaaataaa ggagaagttg aatttcaaaa
//
