LOCUS       NM_001103146            7816 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript
            variant 3, mRNA.
ACCESSION   NM_001103146
VERSION     NM_001103146.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Lv,Z., Ren,Y., Li,Y., Niu,F., Li,Z., Li,M., Li,X., Li,Q., Huang,D.,
            Yu,Y., Xiong,Y. and Qian,L.
  TITLE     RNA-binding protein GIGYF2 orchestrates hepatic insulin resistance
            through STAU1/PTEN-mediated disruption of the PI3K/AKT signaling
            cascade
  JOURNAL   Mol Med 30 (1), 124 (2024)
   PUBMED   39138413
  REMARK    GeneRIF: RNA-binding protein GIGYF2 orchestrates hepatic insulin
            resistance through STAU1/PTEN-mediated disruption of the PI3K/AKT
            signaling cascade.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Xu,Z., Choi,J.H., Dai,D.L., Luo,J., Ladak,R.J., Li,Q., Wang,Y.,
            Zhang,C., Wiebe,S., Liu,A.C.H., Ran,X., Yang,J., Naeli,P.,
            Garzia,A., Zhou,L., Mahmood,N., Deng,Q., Elaish,M., Lin,R.,
            Mahal,L.K., Hobman,T.C., Pelletier,J., Alain,T., Vidal,S.M.,
            Duchaine,T., Mazhab-Jafari,M.T., Mao,X., Jafarnejad,S.M. and
            Sonenberg,N.
  TITLE     SARS-CoV-2 impairs interferon production via NSP2-induced
            repression of mRNA translation
  JOURNAL   Proc Natl Acad Sci U S A 119 (32), e2204539119 (2022)
   PUBMED   35878012
REFERENCE   3  (bases 1 to 7816)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 7816)
  AUTHORS   Zinshteyn,B., Sinha,N.K., Enam,S.U., Koleske,B. and Green,R.
  TITLE     Translational repression of NMD targets by GIGYF2 and EIF4E2
  JOURNAL   PLoS Genet 17 (10), e1009813 (2021)
   PUBMED   34665823
  REMARK    GeneRIF: Translational repression of NMD targets by GIGYF2 and
            EIF4E2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7816)
  AUTHORS   Saini,P., Rudakou,U., Yu,E., Ruskey,J.A., Asayesh,F., Laurent,S.B.,
            Spiegelman,D., Fahn,S., Waters,C., Monchi,O., Dauvilliers,Y.,
            Dupre,N., Greenbaum,L., Hassin-Baer,S., Espay,A.J., Rouleau,G.A.,
            Alcalay,R.N., Fon,E.A., Postuma,R.B. and Gan-Or,Z.
  TITLE     Association study of DNAJC13, UCHL1, HTRA2, GIGYF2, and EIF4G1 with
            Parkinson's disease
  JOURNAL   Neurobiol Aging 100, 119 (2021)
   PUBMED   33239198
  REMARK    GeneRIF: Association study of DNAJC13, UCHL1, HTRA2, GIGYF2, and
            EIF4G1 with Parkinson's disease.
REFERENCE   6  (bases 1 to 7816)
  AUTHORS   Tan,E.K. and Schapira,A.H.
  TITLE     Summary of GIGYF2 studies in Parkinson's disease: the burden of
            proof
  JOURNAL   Eur J Neurol 17 (2), 175-176 (2010)
   PUBMED   19906271
REFERENCE   7  (bases 1 to 7816)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   8  (bases 1 to 7816)
  AUTHORS   Giovannone,B., Lee,E., Laviola,L., Giorgino,F., Cleveland,K.A. and
            Smith,R.J.
  TITLE     Two novel proteins that are linked to insulin-like growth factor
            (IGF-I) receptors by the Grb10 adapter and modulate IGF-I signaling
  JOURNAL   J Biol Chem 278 (34), 31564-31573 (2003)
   PUBMED   12771153
REFERENCE   9  (bases 1 to 7816)
  AUTHORS   Pankratz,N., Nichols,W.C., Uniacke,S.K., Halter,C., Rudolph,A.,
            Shults,C., Conneally,P.M. and Foroud,T.
  CONSRTM   Parkinson Study Group
  TITLE     Significant linkage of Parkinson disease to chromosome 2q36-37
  JOURNAL   Am J Hum Genet 72 (4), 1053-1057 (2003)
   PUBMED   12638082
REFERENCE   10 (bases 1 to 7816)
  AUTHORS   Margolis,R.L., Abraham,M.R., Gatchell,S.B., Li,S.H., Kidwai,A.S.,
            Breschel,T.S., Stine,O.C., Callahan,C., McInnis,M.G. and Ross,C.A.
  TITLE     cDNAs with long CAG trinucleotide repeats from human brain
  JOURNAL   Hum Genet 100 (1), 114-122 (1997)
   PUBMED   9225980
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK001739.1, BX537885.1,
            BX538321.1, BC008072.2 and AC016692.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 2, 2019 this sequence version replaced NM_001103146.2.
            
            Summary: This gene contains CAG trinucleotide repeats and encodes a
            protein containing several stretches of polyglutamine residues. The
            encoded protein may be involved in the regulation of tyrosine
            kinase receptor signaling. This gene is located in a chromosomal
            region that was genetically linked to Parkinson disease type 11,
            and mutations in this gene were thought to be causative for this
            disease. However, more recent studies in different populations have
            been unable to replicate this association. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, May
            2013].
            
            Transcript Variant: This variant (3) lacks an alternate exon in the
            5' UTR and lacks an exon in the coding region, but maintains the
            reading frame, compared to variant 1. The encoded isoform (b) is
            shorter than isoform a. Both variants 2 and 3 encode the same
            isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX538321.1, BX537885.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373563.9/ ENSP00000362664.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AK001739.1         1-39
            40-3110             BX537885.1         1-3071
            3111-3628           BX538321.1         3093-3610
            3629-3631           BC008072.2         299-301
            3632-3822           BX538321.1         3614-3804
            3823-5682           BX537885.1         3781-5640
            5683-7816           AC016692.9         55216-57349
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.1"
     gene            1..7816
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="GRB10 interacting GYF protein 2"
                     /db_xref="GeneID:26058"
                     /db_xref="HGNC:HGNC:11960"
                     /db_xref="MIM:612003"
     exon            1..62
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            63..128
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            129..212
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     CDS             172..4071
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="isoform b is encoded by transcript variant 3; PERQ
                     amino acid rich, with GYF domain 3; PERQ amino acid-rich
                     with GYF domain-containing protein 2; trinucleotide
                     repeat-containing gene 15 protein; Parkinson disease
                     (autosomal recessive, early onset) 11"
                     /codon_start=1
                     /product="GRB10-interacting GYF protein 2 isoform b"
                     /protein_id="NP_001096616.1"
                     /db_xref="CCDS:CCDS33401.1"
                     /db_xref="GeneID:26058"
                     /db_xref="HGNC:HGNC:11960"
                     /db_xref="MIM:612003"
                     /translation="MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYG
                     REEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVL
                     RLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFGRGGGREMHRSQS
                     WEERGDRRFEKPGRKDVGRPNFEEGGPTSVGRKHEFIRSESENWRIFREEQNGEDEDG
                     GWRLAGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSG
                     SIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVDEGEEC
                     SDSEGSHNEEAKEPDKTNKKEGEKTDRVGVEASEETPQTSSSSARPGTPSDHQSQEAS
                     QFERKDEPKTEQTEKAEEETRMENSLPAKVPSRGDEMVADVQQPLSQIPSDTASPLLI
                     LPPPVPNPSPTLRPVETPVVGAPGMGSVSTEPDDEEGLKHLEQQAEKMVAYLQDSALD
                     DERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMS
                     LLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQH
                     LQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQTLKMRISDQNIIPSVTRS
                     VSVPDTGSIWELQPTASQPTVWEGGSVWDLPLDTTTPGPALEQLQQLEKAKAAKLEQE
                     RREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQR
                     EQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEE
                     AQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQ
                     QQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELM
                     KALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRAR
                     NNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAVKEVGP
                     RNSTNKNKNNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHAL
                     NTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQQR
                     QQQQLPQQQQQQPPQQPPQQPQQQDSVWGMNHSTLHSVFQTNQSNNQQSNFEAVQSGK
                     KKKKQKMVRADPSLLGFSVNASSERLNMGEIETLDDY"
     exon            213..342
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            343..438
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            439..550
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            551..662
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            663..703
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            704..883
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1101
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1264
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1453
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1650
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1810
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1977
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2069
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2177
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2278
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2379
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2541
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2700
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2937
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2938..3060
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            3061..3270
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3476
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3631
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            3632..3855
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4003
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     exon            4004..7816
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5801..5806
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="hexamer: AATAAA"
     regulatory      5817..5822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="hexamer: AATAAA"
     polyA_site      5823
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="major polyA site"
     polyA_site      5838
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
     regulatory      7764..7769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="hexamer: AATAAA"
     regulatory      7788..7793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
                     /note="hexamer: ATTAAA"
     polyA_site      7788
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
     polyA_site      7816
                     /gene="GIGYF2"
                     /gene_synonym="GYF2; PARK11; PERQ2; PERQ3; TNRC15"
ORIGIN      
        1 atcttgtgtt gttgaggctg aggactgact ggggttctga gactccctgt cccggaccgc
       61 agcgttaaaa ggatctgaac aaagtctgct caaatctcct gctgtgaacc agcagaattt
      121 ttgaacaggt ttcttcacat ataaaaatct attgtaaaaa tacggaaaag aatggcagcg
      181 gaaacgcaga cactgaactt tgggcctgaa tggctccgag ctctgtccag tggtgggagt
      241 attacatccc ctcctctttc tccagcattg ccgaagtata aattagcaga ttatcgttac
      301 ggcagagaag aaatgttagc acttttcctt aaagacaaca agataccttc agaccttctg
      361 gataaagaat ttctgcctat cctccaggag gaaccccttc caccattggc tctggtaccc
      421 tttacagaag aagaacagag aaacttttcc atgtctgtaa atagtgctgc tgtcctgcga
      481 ttgacaggac gaggaggagg aggaacagtg gtgggggctc ctagaggtcg aagttcttca
      541 agagggcgag gcagaggcag aggtgaatgt ggtttctacc aaagaagttt tgatgaagta
      601 gagggtgttt ttggtcgagg aggtggcaga gaaatgcata gatcgcagag ctgggaggaa
      661 aggggtgaca gacgttttga aaaaccagga cgaaaagatg tagggagacc aaattttgag
      721 gaaggtggac caacatcagt agggagaaag catgaattta tacgctcaga aagtgaaaat
      781 tggcgcatct ttagagagga acaaaatgga gaagatgaag atggaggttg gcgactagct
      841 ggatcaagga gggatggaga gaggtggcga cctcacagtc ctgatggccc tcgttctgca
      901 ggctggcggg aacacatgga acgacgtcgg aggtttgagt ttgattttcg agatagagat
      961 gatgaacggg gttaccgaag ggttcgctct ggcagtggga gcatagatga tgacagggat
     1021 agcttgcccg aatggtgctt agaggatgct gaagaagaaa tgggtacatt tgactcatct
     1081 ggagcattcc tttctctaaa aaaagtacag aaagagccta ttccagaaga gcaggagatg
     1141 gacttccggc ctgtggacga aggggaggag tgctctgact ctgagggtag ccataatgaa
     1201 gaggccaaag aacccgataa gacaaataag aaagaaggag agaaaacaga tagagtagga
     1261 gttgaagcta gtgaggaaac tccccagacc tcatcatcat ctgctagacc aggtactcct
     1321 tcagaccatc agtctcagga agcatcacag tttgagagga aagatgaacc aaaaactgag
     1381 caaacggaaa aagctgaaga ggagactcgg atggaaaata gtctaccagc caaagtgccc
     1441 agcagagggg atgaaatggt tgctgatgtc cagcagcccc tgtcgcagat tccttcagat
     1501 acagcctctc ctcttctcat acttccacct cctgttccca atcctagtcc tactctccgg
     1561 ccagttgaaa caccagttgt aggtgctcct ggtatgggca gtgtttccac agaacctgat
     1621 gatgaagaag gtctcaaaca tttggagcag caagctgaga aaatggtggc ttatctccaa
     1681 gacagtgcac tagatgatga aagattggca tcaaaactgc aagagcacag agctaaagga
     1741 gtgtcgattc cattgatgca tgaagcaatg cagaagtggt attacaaaga tcctcaggga
     1801 gaaattcaag gtcccttcaa taatcaggag atggcagaat ggtttcaggc gggctatttt
     1861 actatgtctt tattggtgaa gagagcgtgt gatgaaagct tccaacctct tggcgatatc
     1921 atgaaaatgt ggggaagggt tcccttttct ccaggtccag ctccccctcc tcatatggga
     1981 gagctggacc aggaacgact gaccaggcag caagaactca cagccttata ccagatgcag
     2041 cacctgcagt accagcagtt tttaatacaa caacaatatg cacaggtttt ggcccaacag
     2101 cagaaagcag cactgtcttc ccagcagcag cagcagttgg cacttcttct tcaacagttt
     2161 cagaccttga agatgagaat atctgatcag aacatcattc cctcagtaac taggtctgtg
     2221 tccgtgccag atactggctc tatctgggag cttcagccaa cagcttcaca gcctacagtt
     2281 tgggaaggtg gtagtgtatg ggatcttcct ctggacacca cgacaccagg ccctgccctg
     2341 gaacagcttc agcagctaga gaaggccaaa gctgcaaagc tagagcaaga gagaagagag
     2401 gcagaaatga gggcaaaacg ggaagaggaa gagcgaaaga ggcaggaaga actccgaaga
     2461 caacaggagg aaattcttcg gcgacagcag gaagaagaaa ggaaaaggcg agaggaagaa
     2521 gaacttgccc gaaggaaaca ggaagaggct ctgcgtcgcc agcgggagca agaaattgca
     2581 ttaaggcgac agcgagaaga ggaagaaaga cagcagcaag aagaagctct tagaagactg
     2641 gaagagagga gaagagaaga ggaagaaagg cggaagcagg aagaattgtt acgcaaacag
     2701 gaagaggagg ctgcaaaatg ggcccgggaa gaagaagaag cccagcgtcg attagaggag
     2761 aaccggctgc ggatggaaga ggaggcagcc agactccggc atgaggaaga agaacggaag
     2821 agaaaggagc tggaggtcca gcggcagaag gagttaatgc gccagaggca gcagcagcaa
     2881 gaggctctcc ggaggttgca gcagcagcag cagcaacaac agctggcgca gatgaagctt
     2941 ccttcttctt caacgtgggg ccagcagtcc aatacaacag catgtcagtc ccaggccacg
     3001 ctgtcgttgg ctgaaatcca aaaactagag gaagaacgag aacggcagct tcgagaagag
     3061 caaaggcgcc agcagaggga gttgatgaaa gctcttcagc agcagcagca acagcaacag
     3121 cagaaactct caggttgggg gaatgtcagc aaaccttcag gtaccacgaa atctcttctg
     3181 gagatccagc aggaagaggc caggcaaatg caaaagcagc agcagcagca gcagcaacac
     3241 cagcaaccaa acagagctcg taacaatacg cattccaacc tgcacaccag cattgggaat
     3301 tctgtttggg gctctataaa tactggtcct cctaaccagt gggcatctga cctagtcagt
     3361 agtatttgga gtaatgctga cactaaaaac tccaacatgg gattctggga tgatgcagtg
     3421 aaagaggtgg gacctaggaa ttcaacaaat aaaaataaaa acaacgccag tctcagtaaa
     3481 tctgtaggtg tgtctaaccg gcagaataag aaagtagaag aagaagaaaa gttgctgaag
     3541 ctctttcagg gagtaaataa agcccaagat ggatttacgc agtggtgtga acagatgctt
     3601 catgccctta atacggcaaa taacttggat gttcccacat ttgtttcttt cctgaaagaa
     3661 gtagaatctc cttatgaggt ccatgattat atcagggcct atttaggaga tacttctgag
     3721 gccaaggagt ttgccaagca gttccttgag cgccgtgcca aacagaaagc caaccagcag
     3781 cgtcagcagc agcagctgcc acagcagcag cagcagcagc cgccacagca gccgccacag
     3841 cagccacaac agcaggactc tgtgtggggg atgaaccaca gtacactcca ttcagtattt
     3901 cagaccaatc aaagcaacaa ccaacaatcc aattttgagg ctgtgcagag tggcaagaag
     3961 aagaaaaagc agaagatggt ccgagcagat cccagtttat taggattttc agtcaatgca
     4021 tcatcggagc gactcaacat gggtgaaatc gagacgttgg atgactactg agcacctgcc
     4081 agtggactgg ccatccctct cctgtctgcc gactatggag tctccacctt tggacacaac
     4141 acttactcac catttactct ttatcactct gcaacaaatc acagaaccga tcatctcagg
     4201 ctttttcttc tggccctttg tgtccaagat tctttaatcc atttttgttg gtgaacatct
     4261 cagactatag ataagtggac tggaccctgt gtcttggggg tggcagttgg gattactccc
     4321 caacaaggct gattttaggc agcatgtgtt cactgtgctg tgatttcatc tactgtctcc
     4381 cagaaagtgt gttgggatcg gccattagca gcttgctttc tcttgtcact ttttttcttc
     4441 tattttgttt tttcttcttc tttttccccc catcagggca aatggtctaa ctggtgcaat
     4501 catgaagaga gttaatggtt aacagacatt ggccaataac aaaacacccc atggactgtg
     4561 actcgagtat ccaacaggca gtcagagctc tcccggtctg aaagttgcat tgccactgct
     4621 aactttggga ttgcatcaga gaggccctga gtggggttga gatgaggttg gtttggtttg
     4681 atgttacaca ctcctcacct gttctttctg agtgtccttt ctctgaaagg atttatgttt
     4741 ttcttcgtta gatagtgact tctgagcaag ctgatctccc ctggcatgct ccaacctgat
     4801 tggacaaagg aagctctatg gcctgggaga gagactattc ttaatttttc tttcttacaa
     4861 aaactgattt ttcccataaa tatttttact tcagaggact aggaccattt tgttttgggc
     4921 ccttctgctg aaaatttgtc tcgtttaaga ggcagctaga atctttacca tatgtatgaa
     4981 tttgtataat ttcatttttg gatagggata aacttttgct tctgataaaa gcctggaatt
     5041 tcatctggtc ctcagagcat tgcgtgtgtg tcttgctgta gcccggaaaa ggttttgtgt
     5101 aaagattctg ggatggcaag ttgtttgcct tttctgaaaa gagaacatac agaacctgtc
     5161 catctttaag accttcatcc atggaatcta ctatacagga ggatgcagtg ggctggaggg
     5221 gatgggcgaa aatgggagca ggaagcctgg cctggcttct ggtcatggcc tcctaaaacc
     5281 ttaaacttca agtagaaatg tactcaagcc ctatttataa acaaatactt ttcctgcctc
     5341 caccaaaccc ctacagaaca tcacctggaa ttgccactca cactgggttg gagtcattgg
     5401 gcagctgtgc ctgtgcgaga ggtgctgtgg tctgggcagc ccctggaaaa gcacctttgc
     5461 tgcctgtcat tgttgcctga agaaggctgg agttgctctg agagcagttt gggtttggag
     5521 tattatattt ggcttctatt tttattattt tggatcacca ttctccctat cccttcttgc
     5581 ctccctccct tctaaacatg tgtaataact atacagagac tgctacaaaa ttgtatatag
     5641 tttttggatc aaatagcatg aggggagagg aaaccattaa aagttggggc tcctactctc
     5701 ctttgctttg taaattcaaa agttgggggt gggtaagagg gatagttaaa atgtttacaa
     5761 aactttaggc tccctcggaa cttttgccag tgtggaggaa aataaaaaag aacttaaata
     5821 aaatctgatt gtattctatc tgagtgcacc tcttgtactc acctttatgg aggctgagtt
     5881 ctgcactaaa ctgttcctct tggtaccatg gaaaagctcc aagcacccaa gacatggagg
     5941 cagccatggc ttctttctct gccagaccac gtagcactgg ctggttctgt atttgagaat
     6001 gtagaggtca aggcagatgt cggtaagttt gaccaaatat cttttctctt tttgactctc
     6061 cctttctgct aacatgtgga tcagctcctg ccctcatacc gcagacacac ccactctgaa
     6121 cacacccctc cagagcttgc ccacaccttc ccaactagac tctgtgtgtg tattcaggaa
     6181 aaatgaaaat aaatttttaa aaattgggag tggaatgggc agattagggg gaacaaagtc
     6241 attttggtca tggaacatcc atttgcttaa ccaaggagac ttgcttctat ctgaggaatt
     6301 tcccacttag tagaaagaaa cactcttccc tgggaatgtt tgtttctgtc tctctcaatg
     6361 ctactctctt tcatattctc atttgctcac cctttcactt agagccaccg ttttctcatc
     6421 ccattctatg aacttatttc caaattctcc taactattcc tgagttttga agtatttgtt
     6481 ttgtaccctc tctgcttccg gttttttgtt tggttggctt ttttggagat agggtcttgt
     6541 gatgttgccc acactggtct cgagcccctg ggctcaagtg atcctcccgc ctcagcctcc
     6601 taaatatcta ggactacagg tgtgtgccac catgcccagc tccagttttg ttggttgatt
     6661 tgtttaaatt cagagattta aaaacatccc agacaagaca gtttgattat ggagactgtc
     6721 cccaagcaga ttggtgtgct ggtggctgct gtgcataaca gattccctcc agattggctt
     6781 acaacagcta cttcattctg tgggtcagga atttggatag ggctcagctg ggtggtggtt
     6841 ctcctggggt attctttggt tgtggtcaga tgtcttccag ggcatcattc ctatgaaggc
     6901 ttaatgaatt aggcaagctg tccaagatgg ctcactcgtg taatgacagt tgacgttggc
     6961 tgtaggctgg gaactcagct gggttgatga ctggaggagc ttatgtgtgt cgtctccgga
     7021 atggcagctt cagaggagtt gaacttaagt ggtgattggc ttcccccaga gaaccaggca
     7081 gaagctgtgt tacctttttg agctcttagc tcaggagtca catagagtca cttctgtgac
     7141 actgtgttgc atcaaagtag tcacaaaccc actcagattc aagagaaggg agcatggccc
     7201 tctccctgca accccctccc cccccgcctt gatgaaagga gtgacaaggt cacattggag
     7261 aagagcttgt ggggtgggag atattatagc catttttggg aaatatctgc ataaaatgag
     7321 tttatgcata catgaaggac tttattttca ttggctttaa gttgagggtt tttgtttttg
     7381 tttttgtatt ggagatgggt tcttactatg ttgcccaggc tggtctcaaa cccctgggct
     7441 caagtgatcc tgttgcctca gcctcccaag tagctgggac tacagctatg tgccagtgca
     7501 cccagcttgt tttttttaaa ttttaggata gataatggta cttctggctt tatttaaata
     7561 atttttttag ggaaaccatt gtatttatac atgcatatat ttttatatat acaaatatgt
     7621 atataaaagt atatgtttta cattttcaat atatatataa tatatacata tatgttatat
     7681 acgtatatat attgcaccct actttaaaat tgactgataa caaaattggt aattttgtat
     7741 aaaatgccca actgtgcatt attaataaaa gtatgtgaat gactctaatt aaacagaagt
     7801 gattctttga aatgaa
//
