LOCUS       NM_001142269            7385 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens gamma-glutamyl carboxylase (GGCX), transcript variant
            2, mRNA.
ACCESSION   NM_001142269 XR_109950
VERSION     NM_001142269.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Ilut,S., Vesa,S.C., Vacaras,V., Sipos-Lascu,D., Barsan,C.,
            Pop,R.M., Crisan,S., Macarie,A.E., Coada,C.A., Perju-Dumbrava,L.,
            Muresanu,D.F. and Buzoianu,A.D.
  TITLE     Association among VKORC1 rs9923231, CYP4F2 rs2108622, GGCX
            rs11676382 polymorphisms and acute ischemic stroke
  JOURNAL   Medicine (Baltimore) 102 (34), e34836 (2023)
   PUBMED   37653796
  REMARK    GeneRIF: Association among VKORC1 rs9923231, CYP4F2 rs2108622, GGCX
            rs11676382 polymorphisms and acute ischemic stroke.
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Shen,G., Liu,H., Shen,Y. and Xi,S.
  TITLE     [Partial research progress of GGCX pathogenic variation associated
            phenotypes]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 39 (6), 646-650 (2022)
   PUBMED   35773773
  REMARK    GeneRIF: [Partial research progress of GGCX pathogenic variation
            associated phenotypes].
REFERENCE   3  (bases 1 to 7385)
  AUTHORS   Ghosh,S., Kraus,K., Biswas,A., Muller,J., Forin,F., Singer,H.,
            Honing,K., Hornung,V., Watzka,M., Oldenburg,J. and
            Czogalla-Nitsche,K.J.
  TITLE     GGCX variants leading to biallelic deficiency to gamma-carboxylate
            GRP cause skin laxity in VKCFD1 patients
  JOURNAL   Hum Mutat 43 (1), 42-55 (2022)
   PUBMED   34816548
  REMARK    GeneRIF: GGCX variants leading to biallelic deficiency to
            gamma-carboxylate GRP cause skin laxity in VKCFD1 patients.
REFERENCE   4  (bases 1 to 7385)
  AUTHORS   Schmidt-Krey,I., Haase,W., Mutucumarana,V., Stafford,D.W. and
            Kuhlbrandt,W.
  TITLE     Two-dimensional crystallization of human vitamin K-dependent
            gamma-glutamyl carboxylase
  JOURNAL   J Struct Biol 157 (2), 437-442 (2007)
   PUBMED   16979907
  REMARK    GeneRIF: Crystallization of human GGCX.
REFERENCE   5  (bases 1 to 7385)
  AUTHORS   Wu,S.M., Stafford,D.W., Frazier,L.D., Fu,Y.Y., High,K.A., Chu,K.,
            Sanchez-Vega,B. and Solera,J.
  TITLE     Genomic sequence and transcription start site for the human
            gamma-glutamyl carboxylase
  JOURNAL   Blood 89 (11), 4058-4062 (1997)
   PUBMED   9166845
REFERENCE   6  (bases 1 to 7385)
  AUTHORS   Kuo,W.L., Stafford,D.W., Cruces,J., Gray,J. and Solera,J.
  TITLE     Chromosomal localization of the gamma-glutamyl carboxylase gene at
            2p12
  JOURNAL   Genomics 25 (3), 746-748 (1995)
   PUBMED   7759116
REFERENCE   7  (bases 1 to 7385)
  AUTHORS   Zhang,L., Jhingan,A. and Castellino,F.J.
  TITLE     Role of individual gamma-carboxyglutamic acid residues of activated
            human protein C in defining its in vitro anticoagulant activity
  JOURNAL   Blood 80 (4), 942-952 (1992)
   PUBMED   1498334
REFERENCE   8  (bases 1 to 7385)
  AUTHORS   Wu,S.M., Cheung,W.F., Frazier,D. and Stafford,D.W.
  TITLE     Cloning and expression of the cDNA for human gamma-glutamyl
            carboxylase
  JOURNAL   Science 254 (5038), 1634-1636 (1991)
   PUBMED   1749935
REFERENCE   9  (bases 1 to 7385)
  AUTHORS   Brenner,B., Tavori,S., Zivelin,A., Keller,C.B., Suttie,J.W.,
            Tatarsky,I. and Seligsohn,U.
  TITLE     Hereditary deficiency of all vitamin K-dependent procoagulants and
            anticoagulants
  JOURNAL   Br J Haematol 75 (4), 537-542 (1990)
   PUBMED   2145029
REFERENCE   10 (bases 1 to 7385)
  AUTHORS   Borowski,M., Furie,B.C. and Furie,B.
  TITLE     Distribution of gamma-carboxyglutamic acid residues in partially
            carboxylated human prothrombins
  JOURNAL   J Biol Chem 261 (4), 1624-1628 (1986)
   PUBMED   3944102
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC296316.1, AK297397.1,
            BC013979.2, AC016753.9, BM974022.1 and BQ000150.1.
            
            On Jun 1, 2019 this sequence version replaced NM_001142269.3.
            
            Summary: This gene encodes an integral membrane protein of the
            rough endoplasmic reticulum that carboxylates glutamate residues of
            vitamin K-dependent proteins to gamma carboxyl glutamate, a
            modification that is required for their activity. The vitamin
            K-dependent protein substrates have a propeptide that binds the
            enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting
            as co-substrates. Vitamin K-dependent proteins affect a number of
            physiologic processes including blood coagulation, prevention of
            vascular calcification, and inflammation. Allelic variants of this
            gene have been associated with pseudoxanthoma elasticum-like
            disorder with associated multiple coagulation factor deficiency.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 1. It encodes
            isoform 2, which is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK297397.1, SRR14038195.927238.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                DC296316.1         42-101
            61-1869             AK297397.1         42-1850
            1870-3025           BC013979.2         2004-3159
            3026-7166           AC016753.9         111898-116038       c
            7167-7250           BM974022.1         14-97               c
            7251-7297           BQ000150.1         107-153             c
            7298-7298           AC016753.9         111766-111766       c
            7299-7385           BQ000150.1         19-105              c
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..7385
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /note="gamma-glutamyl carboxylase"
                     /db_xref="GeneID:2677"
                     /db_xref="HGNC:HGNC:4247"
                     /db_xref="MIM:137167"
     exon            1..108
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..2171
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /EC_number="4.1.1.90"
                     /note="isoform 2 is encoded by transcript variant 2;
                     vitamin K-dependent gamma-carboxylase; peptidyl-glutamate
                     4-carboxylase"
                     /codon_start=1
                     /product="vitamin K-dependent gamma-carboxylase isoform 2"
                     /protein_id="NP_001135741.1"
                     /db_xref="CCDS:CCDS46353.1"
                     /db_xref="GeneID:2677"
                     /db_xref="HGNC:HGNC:4247"
                     /db_xref="MIM:137167"
                     /translation="MAVSAGSARTSPSSGFLMVLDIPQERGLSSLDRKYLDGLDVCRF
                     PLLDALRPLPLDWMYLVYTIMFLGALGMMLGLCYRISCVLFLLPYWYVFLLDKTSWNN
                     HSYLYGLLAFQLTFMDANHYWSVDGLLNAHRRNAHVPLWNYAVLRGQIFIVYFIAGVK
                     KLDADWVEGYSMEYLSRHWLFSPFKLLLSEELTSLLVVHWGGLLLDLSAGFLLFFDVS
                     RSIGLFFVSYFHCMNSQLFSIGMFSYVMLASSPLFCSPEWPRKLVSYCPRRLQQLLPL
                     KAAPQPSVSCVYKRSRGKSGQKPGLRHQLGAAFTLLYLLEQLFLPYSHFLTQGYNNWT
                     NGLYGYSWDMMVHSRSHQHVKITYRDGRTGELGYLNPGVFTQSRRWKDHADMLKQYAT
                     CLSRLLPKYNVTEPQIYFDIWVSINDRFQQRIFDPRVDIVQAAWSPFQRTSWVQPLLM
                     DLSPWRAKLQEIKSSLDNHTEVVFIADFPGLHLENFVSEDLGNTSIQLLQGEVTVELV
                     AEQKNQTLREGEKMQLPAGEYHKVYTTSPSPSCYMYVYVNTTELALEQDLAYLQELKE
                     KVENGSETGPLPPELQPLLEGEVKGGPEPTPLVQTFLRRQQRLQEIERRRNTPFHERF
                     FRFLLRKLYVFRRSFLMTCISLRNLILGRPSLEQLAQEVTYANLRPFEAVGELNPSNT
                     DSSHSNPPESNPDPVHSEF"
     exon            109..267
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            268..433
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            434..512
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            513..619
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..783
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            784..1049
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1181
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1333
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1503
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1634
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1782
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1978
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..7385
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2252
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /note="major polyA site"
     regulatory      3013..3018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /note="hexamer: ATTAAA"
     polyA_site      3035
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /note="major polyA site"
     regulatory      7365..7370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /note="hexamer: AATAAA"
     polyA_site      7385
                     /gene="GGCX"
                     /gene_synonym="VKCFD1"
                     /note="major polyA site"
ORIGIN      
        1 aaattctcct ggcggcctcc gttcagacgc ggcagctgtg acccacctgc ctcctccgca
       61 gagcaatggc ggtgtctgcc gggtccgcgc ggacctcgcc cagctcaggg ttcttgatgg
      121 tgctagacat tccccaggag cgggggctca gctctctgga ccggaaatac cttgatgggc
      181 tggatgtgtg ccgcttcccc ttgctggatg ccctacgccc actgccactt gactggatgt
      241 atcttgtcta caccatcatg tttctggggg cactgggcat gatgctgggc ctgtgctacc
      301 ggataagctg tgtgttattc ctgctgccat actggtatgt gtttctcctg gacaagacat
      361 catggaacaa ccactcctat ctgtatgggt tgttggcctt tcagctaaca ttcatggatg
      421 caaaccacta ctggtctgtg gacggtctgc tgaatgccca taggaggaat gcccacgtgc
      481 ccctttggaa ctatgcagtg ctccgtggcc agatcttcat tgtgtacttc attgcgggtg
      541 tgaaaaagct ggatgcagac tgggttgaag gctattccat ggaatatttg tcccggcact
      601 ggctcttcag tcccttcaaa ctgctgttgt ctgaggagct gactagcctg ctggtcgtgc
      661 actggggtgg gctgctgctt gacctctcag ctggtttcct gctctttttt gatgtctcaa
      721 gatccattgg cctgttcttt gtgtcctact tccactgcat gaattcccag cttttcagca
      781 ttggtatgtt ctcctacgtc atgctggcca gcagccctct cttctgctcc cctgagtggc
      841 ctcggaagct ggtgtcctac tgcccccgaa ggttgcaaca actgttgccc ctcaaggcag
      901 cccctcagcc cagtgtttcc tgtgtgtata agaggagccg gggcaaaagt ggccagaagc
      961 cagggctgcg ccatcagctg ggagctgcct tcaccctgct ctacctcctg gagcagctat
     1021 tcctgcccta ttctcatttt ctcacccagg gctataacaa ctggacaaat gggctgtatg
     1081 gctattcctg ggacatgatg gtgcactccc gctcccacca gcacgtgaag atcacctacc
     1141 gtgatggccg cactggcgaa ctgggctacc ttaaccctgg ggtatttaca cagagtcggc
     1201 gatggaagga tcatgcagac atgctgaagc aatatgccac ttgcctgagc cgcctgcttc
     1261 ccaagtataa tgtcactgag ccccagatct actttgatat ttgggtctcc atcaatgacc
     1321 gcttccagca gaggattttt gaccctcgtg tggacatcgt gcaggccgct tggtcaccct
     1381 ttcagcgcac atcctgggtg caaccactct tgatggacct gtctccctgg agggccaagt
     1441 tacaggaaat caagagcagc ctagacaacc acactgaggt ggtcttcatt gcagatttcc
     1501 ctggactgca cttggagaat tttgtgagtg aagacctggg caacactagc atccagctgc
     1561 tgcaggggga agtgactgtg gagcttgtgg cagaacagaa gaaccagact cttcgagagg
     1621 gagaaaaaat gcagttgcct gctggtgagt accataaggt gtatacgaca tcacctagcc
     1681 cttcttgcta catgtacgtc tatgtcaaca ctacagagct tgcactggag caagacctgg
     1741 catatctgca agaattaaag gaaaaggtgg agaatggaag tgaaacaggg cctctacccc
     1801 cagagctgca gcctctgttg gaaggggaag taaaaggggg ccctgagcca acacctctgg
     1861 ttcagacctt tcttagacgc caacaaaggc tccaggagat tgaacgccgg cgaaatactc
     1921 ctttccatga gcgattcttc cgcttcttgt tgcgaaagct ctatgtcttt cgccgcagct
     1981 tcctgatgac ttgtatctca cttcgaaatc tgatattagg ccgtccttcc ctggagcagc
     2041 tggcccagga ggtgacttat gcaaacttga gaccctttga ggcagttgga gaactgaatc
     2101 cctcaaacac ggattcttca cattctaatc ctcctgagtc aaatcctgat cctgtccact
     2161 cagagttctg aagggggcca gatgttgggt gcagatgtag aagcagccag tcacagaccc
     2221 attctatgca atggacattt atttgaaaaa aattctcaaa agtttttttt ttttttttgg
     2281 gggggcgggg ttctaaagct gtttttaact ccgagattac aacttagagg aaccaaggaa
     2341 ataaagcaaa taagatttaa caacccaaga ttaagaggcc aggaagaggt tagacgcaat
     2401 gtgaaactgt cctcctagga taaggtttaa agtggctttt tgggggctgg gtgccgtggc
     2461 tcacgcctgt aatcccagca ttttgggagg ctgaggtggg cagatcactt gaggccagga
     2521 gttcgagacc agcctggcca acatggcaaa accccttctc tactaaaaat acaaaaatta
     2581 gccagacgtg gtggtgggtg cctgtaatcc aactacccag gaggctgagg catgagaatc
     2641 gcttgggccc aggaggtgga ggttgcagtg agccgagatc gagccactgc actcctgggc
     2701 aacagagcaa gacttcgtct caaaataaat aaataaagtg gctcttgggg aaaagcaatt
     2761 taatgtacca cgatgaatag ctaactgttc ccaagtgttt gctatgtgca acacaccgcg
     2821 tgagcagtgt tacctgcatt attacattag gctgagaggt aaaataattt gcccgaagac
     2881 atacagctag tgacgaatgg actgatggtt tgaacttaac gtctatttga cttaaggtcc
     2941 tgcaccctgc cacttgtaat tttcagaatc actgataatc tgaaataatg cagcttaaaa
     3001 catgttttct taattaaaag tataattgga tggtggtcag gttgtaaatg cacacaaagt
     3061 aatttatctt cattgcaatt ttggttcctt ctttgttcct attatctata tacacccagt
     3121 gtttcactag cagttatgag agcagtcaga atagcagcat caaagttaaa taaggaaatc
     3181 tagatgattg ctagattttc tctaaaaata agcttagtat ttggtattat tctagccttt
     3241 ggtgagcaga aggggaggta aagtaagaat taactaaatt tagtaaggga ttagggaatg
     3301 cttagacatt ttgtaggttg gggtatacaa gggtaactca ggaagctcct cagttcagtg
     3361 aaaattcaaa gggttggcac tgagctagga agcgagaatg cagggttatg aaaaaggatc
     3421 cctgctttgg gagctcatgg acttgtagca gagaccggta gtaaattggc agtctcagta
     3481 acttctgtaa atgattgcat ctctttgcag gcttctcttc ccatactctc ccccactcct
     3541 ccttggacag tgttccgggg gttctttcct aagcaactgc tttttcagta tacatacttc
     3601 agatgattgt gtctactcca ggatttccaa cgatgctagg tttctttcct gtgccctaga
     3661 cgtataggtc taagttccac ctggatatcc cacagggcta tcaagctcag ttcatcctaa
     3721 acagaactta cattcatccc caactcagac ttccttattc tttattattg aatggcagct
     3781 ctgtacctta gccaagctgt tatctctagg tcatcagcct tgactcttcc tttttccttg
     3841 catttcttaa aattgccaag tcccatcaat tttacctcct ggatacctct tgaatctatt
     3901 tttttttaaa gacagggttt cactctatca cccaggctgg agttcagtgg cacaatcttg
     3961 gcttactgca gccaccacct cctgggttta aacgattctt ccacttcagc ctcccatgta
     4021 gctgggacta caggtgccca ccaccacgcc caactaattt ttgtattttt agtagagaca
     4081 tggtttccca cattggccag gctggtctca aactcatgac cttaagtatc cacctgcctt
     4141 ggcctcccaa agggctggga ttacaggtgt gagccaccgt gcccagccaa atctgttttc
     4201 attactacct atatctgtat tttcaacctt tgtattagca tctagttagg gatagaccag
     4261 gacagggccg acatgttagt tgtcaagcag attgccaccc tcccccacca ctgaggaggc
     4321 caaatggagt ttggtggaaa atctctaatt cattcaaggt attatatggg caagcaatgg
     4381 ctgtggcaca gggtcccctg attactttgt aagaatgggt gggagggatg ctgattttca
     4441 atccttttgc agatcttctc tcatagtaac acaatagaag atactttgcc actatgttgt
     4501 ttgccaaact cactgtacct acttgactgg taacaaaagt cccaagagtt acttgggaga
     4561 agacacttga aaaagtattt ttctaaatat gggcaaggat gctgcccttc cccaaactgt
     4621 tagtatacca cctctctgac caattttccc aaggaagata ttggggtagg gtgaggtgta
     4681 aagaaaaagt ggtcatgtaa atactggtaa taaaagctgt tcagtggggt agagttgggt
     4741 tgcaactgta gccattctaa aaatgggcta tcaatgtcct tttagccaaa agactttagt
     4801 atacatcctc ctcattctac cctcaggatt tggatctttg cccctgatgg gtatccatcc
     4861 cctttgaaac tttagaaagg caggttatat acagaatgtt caacctaaag acatttcctt
     4921 aagtcatact ttcatgcttt ctaaactata atcaccctta tcatcccctg ctgttatgct
     4981 ttccctctgc tccctgtggc ctaatgttcc tccaccacct tattaacctt caggatactt
     5041 ttcccctagg ctgggcagga caacactatc cccttggtcc acaagctccc tcaaggttgg
     5101 ggaatctttg tttacttagc aaatactgaa cataacctta aagcggttac ttttgaaaac
     5161 ttctcatgct attctaccaa cttggaggca cctatccatg ctcattcccc taatgtagcc
     5221 aatatggtta tctgcaatgt attggcatct aaaaactccc ttatgaaact gccagacttg
     5281 ttctggaaca tttcaattac atgcaaaatt caggttctca agttggaaca agtctaattg
     5341 acacacctgc ataggtttta accttgccct ccccctactt cccaacagat ttccaagttt
     5401 tttgagaagg aaccttgctc tggcccaggc tggagtgcaa tggcgccatc tcggctcact
     5461 gcaacctctg cctcctgggt tcaagtgatt ctccagcctc ctgagtagct gggattacaa
     5521 gcacctgcca ccacacccag cttttttgta tttttggtag agacggggtt tcagtttgcc
     5581 aggctggtat caaaccgtga cctcaggtga tccacccgca ttagcctccc aaagtgctgg
     5641 gattacaggt gtaagctacc atacccagat ttccacggct ttttttttaa gtctcactct
     5701 gttaccctgg ccggagtgtg gtggcacgat ttcagctcac tgcaagctcc acctcccggg
     5761 ttcacgccat tctcctgcct cggcctcctg agtagctggg actacaggtg cctggctaac
     5821 ttttttatat ttttagtaga gacagggttt cactgtgtta gccaggatgg tctcgtgatc
     5881 tgcatgcctt ggcctcccaa agtgctagga ttataggctc acgcctatat tactatatag
     5941 tggcctatat tacaggccac tccgcctggc ctccaaattt ccaagttttt aagtctccat
     6001 gcaaacaggc ataagcaagc actccttggc tctacatttg caggacaagc tggaccacca
     6061 ttcagatgtt ggtagaaatg taatccaagt ggctattctc tatgcatgta gctcctaggt
     6121 tcaagagcag tgtggtctgc ggattttcta cacaacctgc cataaatgaa atttaagcct
     6181 ttggaaattt atagcatttt cagctaattt gcattattta tctctgcaag atattgacta
     6241 gaaatcacgt ttcagaactt ctcagatgaa aacatcatac aacccttaaa actaagctca
     6301 taatttaaaa atgtatgcag attaataggc ttaaattgcc aggactttag atagacatta
     6361 ccacttccag gttaatactt ttagcggttt ttatgccttc aatggtttga agccctcaac
     6421 cttagttgaa aacttaaaaa tggcattgaa tggctgctgc aataaggaca catgaatttt
     6481 ggcttaagtg gactaggagg actaaatctt gcttaggctt ttaaaaaatg cccacccact
     6541 gggtctaggg attgaggtta ttttcctgaa ttatggctaa ttatggatta caattcaagg
     6601 ttaaagatac acatatagta ttctcaggtt attcatatga tagcattttt taagagccaa
     6661 agtctcagtc acccaggctg gagtgcagtg gcttgataat ggttcgctac agcctccaac
     6721 ttctatgtta aagtgatcct cctacctcag cctttcaaag tgttgggatt ataggcacgt
     6781 acctatggcc ccaaagcact ttaacaaatg agtaacagac acagttaagg tagaacctag
     6841 cattttattt agatcttcat taaactgttg gaattgagaa ccagacatac gtaataaacc
     6901 tccaaaaata gatcctgaaa ggcactttct gcttagggca agcagtcatg gaataagcat
     6961 gtaaacaagc tggcttctct gtaccacacc agccaagtca gctttctcca tggccagccg
     7021 caccagctct gccctccctt ctgttaacac cagccagacc ccctgtaggt ctaacccaag
     7081 gtttttctgt aggacacctt ggcctacctg ggaatgctgg aaacatgttg ttaaaggaat
     7141 catggtgttg agagaaaaaa aaaaaatctt ttaaaagctg ccatctgagg tgatggcttc
     7201 tctgtactta cgccataccc cagaatacaa taaataagca attagaaaac gttcaagtat
     7261 gaagggattt cctcctcccc gccaaaagca ctgctctctg aaggaagctg gtttctctgt
     7321 agctacacca gctgttcaga aagctcattg gacctggttt tgaaaataaa acaaagttaa
     7381 aaccc
//
