LOCUS       NM_005264               9161 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens GDNF family receptor alpha 1 (GFRA1), transcript
            variant 1, mRNA.
ACCESSION   NM_005264 NM_001348097
VERSION     NM_005264.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Avenel,I.C.N., Ewald,J.D., Ariey-Bonnet,J., Kristensen,I.H.,
            Petterson,S.A., Thesbjerg,M.N., Burton,M., Thomassen,M.,
            Wennerberg,K., Michaelsen,S.R. and Kristensen,B.W.
  TITLE     GDNF/GFRA1 signaling contributes to chemo- and radioresistance in
            glioblastoma
  JOURNAL   Sci Rep 14 (1), 17639 (2024)
   PUBMED   39085346
  REMARK    GeneRIF: GDNF/GFRA1 signaling contributes to chemo- and
            radioresistance in glioblastoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Shamhari,A.'., Jefferi,N.E.S., Abd Hamid,Z., Budin,S.B.,
            Idris,M.H.M. and Taib,I.S.
  TITLE     The Role of Promyelocytic Leukemia Zinc Finger (PLZF) and
            Glial-Derived Neurotrophic Factor Family Receptor Alpha 1
            (GFRalpha1) in the Cryopreservation of Spermatogonia Stem Cells
  JOURNAL   Int J Mol Sci 24 (3), 1945 (2023)
   PUBMED   36768269
  REMARK    GeneRIF: The Role of Promyelocytic Leukemia Zinc Finger (PLZF) and
            Glial-Derived Neurotrophic Factor Family Receptor Alpha 1
            (GFRalpha1) in the Cryopreservation of Spermatogonia Stem Cells.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9161)
  AUTHORS   Meng,M. and Wu,Y.C.
  TITLE     LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic
            Properties and Immune Escape of Prostate Cancer
  JOURNAL   J Immunol Res 2022, 7375879 (2022)
   PUBMED   35832649
  REMARK    GeneRIF: LMX1B Activated Circular RNA GFRA1 Modulates the
            Tumorigenic Properties and Immune Escape of Prostate Cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9161)
  AUTHORS   Bazhabayi,M., Qiu,X., Li,X., Yang,A., Wen,W., Zhang,X., Xiao,X.,
            He,R. and Liu,P.
  TITLE     CircGFRA1 facilitates the malignant progression of HER-2-positive
            breast cancer via acting as a sponge of miR-1228 and enhancing
            AIFM2 expression
  JOURNAL   J Cell Mol Med 25 (21), 10248-10256 (2021)
   PUBMED   34668628
  REMARK    GeneRIF: CircGFRA1 facilitates the malignant progression of
            HER-2-positive breast cancer via acting as a sponge of miR-1228 and
            enhancing AIFM2 expression.
REFERENCE   5  (bases 1 to 9161)
  AUTHORS   Shefelbine,S.E., Khorana,S., Schultz,P.N., Huang,E., Thobe,N.,
            Hu,Z.J., Fox,G.M., Jing,S., Cote,G.J. and Gagel,R.F.
  TITLE     Mutational analysis of the GDNF/RET-GDNFR alpha signaling complex
            in a kindred with vesicoureteral reflux
  JOURNAL   Hum Genet 102 (4), 474-478 (1998)
   PUBMED   9600247
REFERENCE   6  (bases 1 to 9161)
  AUTHORS   Sanicola,M., Hession,C., Worley,D., Carmillo,P., Ehrenfels,C.,
            Walus,L., Robinson,S., Jaworski,G., Wei,H., Tizard,R., Whitty,A.,
            Pepinsky,R.B. and Cate,R.L.
  TITLE     Glial cell line-derived neurotrophic factor-dependent RET
            activation can be mediated by two different cell-surface accessory
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (12), 6238-6243 (1997)
   PUBMED   9177201
REFERENCE   7  (bases 1 to 9161)
  AUTHORS   Gattei,V., Celetti,A., Cerrato,A., Degan,M., De Iuliis,A.,
            Rossi,F.M., Chiappetta,G., Consales,C., Improta,S., Zagonel,V.,
            Aldinucci,D., Agosti,V., Santoro,M., Vecchio,G., Pinto,A. and
            Grieco,M.
  TITLE     Expression of the RET receptor tyrosine kinase and GDNFR-alpha in
            normal and leukemic human hematopoietic cells and stromal cells of
            the bone marrow microenvironment
  JOURNAL   Blood 89 (8), 2925-2937 (1997)
   PUBMED   9108413
REFERENCE   8  (bases 1 to 9161)
  AUTHORS   Treanor,J.J., Goodman,L., de Sauvage,F., Stone,D.M., Poulsen,K.T.,
            Beck,C.D., Gray,C., Armanini,M.P., Pollock,R.A., Hefti,F.,
            Phillips,H.S., Goddard,A., Moore,M.W., Buj-Bello,A., Davies,A.M.,
            Asai,N., Takahashi,M., Vandlen,R., Henderson,C.E. and Rosenthal,A.
  TITLE     Characterization of a multicomponent receptor for GDNF
  JOURNAL   Nature 382 (6586), 80-83 (1996)
   PUBMED   8657309
REFERENCE   9  (bases 1 to 9161)
  AUTHORS   Jing,S., Wen,D., Yu,Y., Holst,P.L., Luo,Y., Fang,M., Tamir,R.,
            Antonio,L., Hu,Z., Cupples,R., Louis,J.C., Hu,S., Altrock,B.W. and
            Fox,G.M.
  TITLE     GDNF-induced activation of the ret protein tyrosine kinase is
            mediated by GDNFR-alpha, a novel receptor for GDNF
  JOURNAL   Cell 85 (7), 1113-1124 (1996)
   PUBMED   8674117
REFERENCE   10 (bases 1 to 9161)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012470.16 and AC073588.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 21, 2020 this sequence version replaced NM_005264.7.
            
            Summary: This gene encodes a member of the glial cell line-derived
            neurotrophic factor receptor (GDNFR) family of proteins. The
            encoded preproprotein is proteolytically processed to generate the
            mature receptor. Glial cell line-derived neurotrophic factor (GDNF)
            and neurturin (NTN) are two structurally related, potent
            neurotrophic factors that play key roles in the control of neuron
            survival and differentiation. This receptor is a
            glycosylphosphatidylinositol (GPI)-linked cell surface receptor for
            both GDNF and NTN, and mediates activation of the RET tyrosine
            kinase receptor. This gene is a candidate gene for Hirschsprung
            disease. Alternative splicing results in multiple transcript
            variants, at least one of which encodes a preproprotein that is
            proteolytically processed. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Variants 1 and 6
            both encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF042080.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355422.11/ ENSP00000347591.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC012470.16        83937-83980         c
            45-330              AC012470.16        82764-83049         c
            331-624             AC012470.16        81596-81889         c
            625-708             AC012470.16        80277-80360         c
            709-723             AC073588.3         164270-164284       c
            724-1060            AC073588.3         77859-78195         c
            1061-1170           AC073588.3         49293-49402         c
            1171-1305           AC073588.3         46340-46474         c
            1306-1487           AC073588.3         42379-42560         c
            1488-1541           AC073588.3         18211-18264         c
            1542-9161           AC073588.3         9563-17182          c
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q25.3"
     gene            1..9161
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="GDNF family receptor alpha 1"
                     /db_xref="GeneID:2674"
                     /db_xref="HGNC:HGNC:4243"
                     /db_xref="MIM:601496"
     exon            1..44
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            45..330
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..1688
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="isoform a preproprotein is encoded by transcript
                     variant 1; Glial cell line-derived neurotrophic factor
                     receptor alpha; RET ligand 1; GPI-linked anchor protein;
                     PI-linked cell-surface accessory protein; TGF-beta-related
                     neurotrophic factor receptor 1; GDNF receptor alpha 1e;
                     GDNF receptor alpha 1d"
                     /codon_start=1
                     /product="GDNF family receptor alpha-1 isoform a
                     preproprotein"
                     /protein_id="NP_005255.1"
                     /db_xref="CCDS:CCDS44481.1"
                     /db_xref="GeneID:2674"
                     /db_xref="HGNC:HGNC:4243"
                     /db_xref="MIM:601496"
                     /translation="MFLATLYFALPLLDLLLSAEVSGGDRLDCVKASDQCLKEQSCST
                     KYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRI
                     YWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISDVFQQVEHIPKGNNCLDAAKA
                     CNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRD
                     IACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSS
                     CLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKD
                     NTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVL
                     PPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTKSMAAPPSCGLSPLLVL
                     VVTALSTLLSLTETS"
     exon            331..624
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            625..708
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            709..723
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            724..1060
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1170
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1305
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1487
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1541
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..9161
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1701..1706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="hexamer: AATACA"
     polyA_site      1731
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="major polyA site"
     regulatory      2313..2318
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="hexamer: AAGAAA"
     polyA_site      2338
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
     regulatory      2620..2625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="hexamer: AATACA"
     polyA_site      2644
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
     regulatory      3738..3743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="hexamer: AATAAA"
     polyA_site      3766
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
     regulatory      5294..5299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="hexamer: AATAAA"
     polyA_site      5330
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
     regulatory      6485..6490
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
                     /note="hexamer: AATAAA"
     polyA_site      6509
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
     polyA_site      7864
                     /gene="GFRA1"
                     /gene_synonym="GDNFR; GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1;
                     GFRalpha-1; RET1L; RETL1; RHDA4; TRNR1"
ORIGIN      
        1 aaactggctc ctcgccgcag ctggacgcgg tcggttgagt ccaggttggg tcggacctga
       61 acccctaaaa gcggaaccgc ctcccgccct cgccatcccg gagctgagtc gccggcggcg
      121 gtggctgctg ccagacccgg agtttcctct ttcactggat ggagctgaac tttgggcggc
      181 cagagcagca cagctgtccg gggatcgctg cacgctgagc tccctcggca agacccagcg
      241 gcggctcggg atttttttgg gggggcgggg accagccccg cgccggcacc atgttcctgg
      301 cgaccctgta cttcgcgctg ccgctcttgg acttgctcct gtcggccgaa gtgagcggcg
      361 gagaccgcct ggattgcgtg aaagccagtg atcagtgcct gaaggagcag agctgcagca
      421 ccaagtaccg cacgctaagg cagtgcgtgg cgggcaagga gaccaacttc agcctggcat
      481 ccggcctgga ggccaaggat gagtgccgca gcgccatgga ggccctgaag cagaagtcgc
      541 tctacaactg ccgctgcaag cggggtatga agaaggagaa gaactgcctg cgcatttact
      601 ggagcatgta ccagagcctg cagggaaatg atctgctgga ggattcccca tatgaaccag
      661 ttaacagcag attgtcagat atattccggg tggtcccatt catatcagat gtttttcagc
      721 aagtggagca cattcccaaa gggaacaact gcctggatgc agcgaaggcc tgcaacctcg
      781 acgacatttg caagaagtac aggtcggcgt acatcacccc gtgcaccacc agcgtgtcca
      841 acgatgtctg caaccgccgc aagtgccaca aggccctccg gcagttcttt gacaaggtcc
      901 cggccaagca cagctacgga atgctcttct gctcctgccg ggacatcgcc tgcacagagc
      961 ggaggcgaca gaccatcgtg cctgtgtgct cctatgaaga gagggagaag cccaactgtt
     1021 tgaatttgca ggactcctgc aagacgaatt acatctgcag atctcgcctt gcggattttt
     1081 ttaccaactg ccagccagag tcaaggtctg tcagcagctg tctaaaggaa aactacgctg
     1141 actgcctcct cgcctactcg gggcttattg gcacagtcat gacccccaac tacatagact
     1201 ccagtagcct cagtgtggcc ccatggtgtg actgcagcaa cagtgggaac gacctagaag
     1261 agtgcttgaa atttttgaat ttcttcaagg acaatacatg tcttaaaaat gcaattcaag
     1321 cctttggcaa tggctccgat gtgaccgtgt ggcagccagc cttcccagta cagaccacca
     1381 ctgccactac caccactgcc ctccgggtta agaacaagcc cctggggcca gcagggtctg
     1441 agaatgaaat tcccactcat gttttgccac cgtgtgcaaa tttacaggca cagaagctga
     1501 aatccaatgt gtcgggcaat acacacctct gtatttccaa tggtaattat gaaaaagaag
     1561 gtctcggtgc ttccagccac ataaccacaa aatcaatggc tgctcctcca agctgtggtc
     1621 tgagcccact gctggtcctg gtggtaaccg ctctgtccac cctattatct ttaacagaaa
     1681 catcatagct gcattaaaaa aatacaatat ggacatgtaa aaagacaaaa accaagttat
     1741 ctgtttcctg ttctcttgta tagctgaaat tccagtttag gagctcagtt gagaaacagt
     1801 tccattcaac tggaacattt tttttttttc cttttaagaa agcttcttgt gatcctttgg
     1861 ggcttctgtg aaaaacctga tgcagtgctc catccaaact cagaaggctt tgggatatgc
     1921 tgtattttaa agggacagtt tgtaacttgg gctgtaaagc aaactggggc tgtgttttcg
     1981 atgatgatga tgatcatgat gatgatcatc atgatcatga tgatgatcat catgatcatg
     2041 atgatgattt taacagtttt acttctggcc tttcctagct agagaaggag ttaatatttc
     2101 taaggtaact cccatatctc ctttaatgac attgatttct aatgatataa atttcagcct
     2161 acattgatgc caagcttttt tgccacaaag aagattctta ccaagagtgg gctttgtgga
     2221 aacagctggt actgatgttc acctttatat atgtactagc attttccacg ctgatgttta
     2281 tgtactgtaa acagttctgc actcttgtac aaaagaaaaa acacctgtca catccaaata
     2341 tagtatctgt cttttcgtca aaatagagag tggggaatga gtgtgccgat tcaatacctc
     2401 aatccctgaa cgacactctc ctaatcctaa gccttacctg agtgagaagc cctttaccta
     2461 acaaaagtcc aatatagctg aaatgtcgct ctaatactct ttacacatat gaggttatat
     2521 gtagaaaaaa attttactac taaatgattt caactattgg ctttctatat tttgaaagta
     2581 atgatattgt ctcatttttt tactgatggt ttaatacaaa atacacagag cttctttccc
     2641 ctcataagta gtgttcgctc tgatatgaac ttcacaaata cagctcatca aaagcagact
     2701 ctgagaagcc tcgtgctgta gcagaaagtt ctgcatcatg tgactgtgga caggcaggag
     2761 gaaacagaac agacaagcat tgtcttttgt cattgctcga agtgcaagcg tgcatacctg
     2821 tggagggaac tggtggctgc ttgtaaatgt tctgcagcat ctcttgacac acttgtcatg
     2881 acacaatcca gtaccttggt tttcaggtta tctgacaaag gcagctttga ttgggacatg
     2941 gaggcatggg caggttctcc tttctttgga aacacatcca tgctgggatg cacagttctt
     3001 ttcctaagga ttgtgtttac tcctgactgc aacgtcttgg ccactgtttg gagcagtttg
     3061 gtctgaaggt cattaaggat ctgcttgcat cttgtttcat gtcctccgtg acagactgca
     3121 gaccatgtta gaagagaagg aaggtttgtg gatcaagagg gttgacatta ggtttggtga
     3181 gatgagttgc ttggcacaga tcagcagaac aggaccctgg tgataaggca tctggctttg
     3241 ctaaccactt atcatcttcc attattctct ttcttaacta agtagacccg aaagtgcact
     3301 tttgaagtct ggtttagatg gcgagattcc gaatctacaa tgatcgagta ctggtggcca
     3361 cttttctcca acattaagta aagaggagga gattccacct tcagaaagtg ctgggaagca
     3421 ttggctttct tcccacattg gtagaagaga tggaggagga aaccagggtg cacatttcta
     3481 cagatcccct ttgcctgctg gtgtttgcct tgtaagataa gtcactgggg ggaaggggaa
     3541 atctatgaaa tccaagataa aggcatagtc tttcagctct attggtgtta aaagtatatt
     3601 tgtgtccaaa tgttaatcaa cacaaatgta cagtactcag tactgacttt tttcacttca
     3661 aaatgttttc aagggaaatt cagcagacac ccaactgtat ttaattagtg aaaagatcta
     3721 taaagactct tactttgaat aaacacatta agtaagtgtg tcaaaatatt tggaatgcca
     3781 gttatctatg agcgcatatc tcttacagag tgtattttgt tttcattaag gtatctctgt
     3841 gtatctacac tggactaatt tcatagggat ctgattctat gtgatccact gtagaaagct
     3901 actgattact tctgcagtaa cagcagctct aattagcatc agatgaccaa agcggaaatg
     3961 ggagtttcag cgctaacaag ggcagccttc attgaagaat gcatgggtag gcaatatata
     4021 aattacattt tgaaaagcct catctgtcac caacaaatat ctcatttttc ttcagtgata
     4081 aaagcatatt caagttacca gctcctcaat ttgaaaatca gttgctgatt aaataaatag
     4141 ggggaagcat cttgttcagg gacaacacgt tagagagatt cttatcaaag caagccaagc
     4201 aaggtttccg ccattcagtg tgatcttagt ttgacacggt gaccctaaaa caccatcatt
     4261 tgccccagaa ctggagcaca ataagttaaa tcatttacct gtctcacttc ttcttggcgt
     4321 gcttaaaagc aaaaatgatt cgctacttcc tgtccttcag tccgggcatt tggggtgcca
     4381 ctgaagacag gggttatctg ctgaggtttg ggtgtcttgc aggaagtatc tcattgcagt
     4441 tgattttgat gaaaccaaac agtaactagg gtccatgcag accgtgactc cctgattaca
     4501 tctagccatg ccctacttat ttcagagaga aagagagggg gaataaaggc agtattgcca
     4561 cactgattag aagcacattc attccacttt gatgttgaat atttttgacc gatacatgct
     4621 tgagtatctt tttaatagag cacatgaagg aagaagcaca caatatagag aaggaattga
     4681 gaatgccaat tacatattta tttttccata cctgattttt ttcaagtctg taataaaaaa
     4741 agtataagtt gagattaaca taggttattt ttcatgaagt atagcaaacg atctagaatg
     4801 tgataggagt gtggtttcca tttctttttt ttttttttta acagactttt gtgctgtagt
     4861 aagaatgtct tagaagttgt gtcttcgagt tcctcaaaca ccggacaggg ctcccccaga
     4921 gctgcctcag cctttgattt ggtccatatt cagacacttc ctgtcttgca tacactctga
     4981 ggatgggccg cttctagctg ccgcccgtct ccctcttgcc ttcttgtctc taaatcagac
     5041 actcccaagc actccatctg ctatactagg agagtgggag agccaaagca ttttggagtc
     5101 catgtaaagt gcctgggaaa agagatgtaa gagcagtggg agtttatata attagcgaat
     5161 tcttttctca gaattcagcg gaaatgtatt tctgttgtta ttatttacaa aattgtggcc
     5221 tcagaggagc tgtcttttgg aaaataagtt ttcattcatg cagttagtta gacatgcacg
     5281 atgtacttga atcaataaac tgtggaccag aaaagggctg cttaaatgaa ttcacgtgtt
     5341 ctaattctct ttcgaggctg ttgatgacct cctggattca tacccccatg atctgtcttg
     5401 ctcttgcctc tgttctttga taaagcacat cagactatgt gacacccatc ttttcaagaa
     5461 cgacctaaaa gcaatgacaa caggcaggag agaattacca ctggcctggg cacagtgaca
     5521 gcagaaggcc cacctgagct tggagagttg gcgagataag gcaaaatcaa ggtgcaacag
     5581 tgggccatgt tccaagggtg aatggatctg agagtccgct gatgttcctt actgatcaga
     5641 acagggacct tctaacgaaa cagccagagt tccttgaatc aggcagaagt tctttagaga
     5701 caggaagcca gagctattga taatatccga acgacagagg aaggaatttc ttattagaaa
     5761 ttctaaaata aaacaagtgg ggatttggtt ggataccctt agaagctaca tttttctctc
     5821 atccctaaga tagatatctc aagtgtatat ctgacacctc ttggctttga cacggctctg
     5881 cttctcaagt gaccatttgt tttaagtctg ttgtctagaa ttctttgtgg gggctggacc
     5941 tctgaaaaag attctcactg cagaggcttt aaatctgtgt ttttttccac tccctccgtc
     6001 ctgggatgta ttcggtagga catttcattt atatcaggat attggctgat tcagctctct
     6061 ttcatcgtta ttattcaggc ctccctccac caccttgttc tttattgcct aagtattgcc
     6121 ctccccaatt cttcatcgta atagctctaa atttagtttg acccaaatta atttttgttc
     6181 caacagatat attttaagac agagaagcct ctctcaggag ccagatgatc tgaagatgat
     6241 gctaatcccc aaatacgaag ttcctccagc taactctcct ctgagctccc tcttcttagc
     6301 tttgggattg gggatggtct catccttgcc aggaatccct ttgttgctcc cagtgtctct
     6361 gatgagcttc tcctccctcc cagagacaat gcaggggtgg ttttcagcag aatgtgccac
     6421 ctgcacttct gattgttctg agacccagcc cagaggggtg gagaaggcgc acaaacccta
     6481 ttccaataaa ctgcctgcag agaaccacag cctgctaata gtcgttgagg tctcagcccc
     6541 ctaaggtctc caagctcatt tgaccagcgc agctgcgact tcggctgtct gcctctggca
     6601 ggtgccaata tcagcatctt gtcccactgc catggggagt taatttttgc tccagggcag
     6661 ctgccaagcc cagcaccggc atcaatagaa ggttttctct gattttcttc tcttcctctc
     6721 aggctaggct ttcttaccaa tccccaattt tcttctgtag ggcgccagga gactggccag
     6781 agatctttag actcttactt agtttgaaca tctcttgctg ctcacaccca ggcttcttca
     6841 gcagaaagca tgacccatag taggtactca gaaactggtt gaatgaaaag ccttgacatt
     6901 taagggcttt attctgtcat acggtagcaa gaactggcct attggcatcg tattcctggc
     6961 attgagctgt attgaccctg aatgtgtcct cctgaggttt ttgagggtga cctaggagaa
     7021 agggtctgaa aaagcactgg gatccaagtg actttaaatc tgcccgtggc atttcctgct
     7081 gagaggcgag aaactagcat ctctcccaac aggctcacat ccgccaggaa ggacagagcc
     7141 caaaacgggg gtagactttg tgggaaattc gccagttagt ccgctgagat tttaatctgc
     7201 ctcaagcaca ggatgctgac aagaaaggta gaccactccc ccgatcccag acaaacccca
     7261 ggggtgacaa cttctccatc agtatcctga gaagcaacat caaatgctta ccaagtcagc
     7321 acgtttactg agtgagtact ctgacggaat aaaccagcat ttggagctga acttgagaac
     7381 acagggccct ctgacagcag cttagggaaa cacccatccc ttccagattg gcagaatcct
     7441 tccatttcat gtccttcgga gacgctgtat tctctttaca aatcattcat gaaactgaga
     7501 ctcagtctcc caggggtaag tcctgggcca cgtgcaaaca atggcaaatc cagctcttcc
     7561 atgatagaga aggcaaacac acccaccttc atcctgagcc taaaaggcca cctctgagca
     7621 cttgggcagc cactcctctg ggcctcagag ggccatgagc ttggccaggt aggcacagcg
     7681 gcggggaagt cacagctgtc aggtaccggc catggtgcag gtgggaatag gagatgccag
     7741 agctgcttta gctgaggaaa gcaaacagtc agcagtgctc aaaggagcaa aacttcgaat
     7801 gtgcacattg acccctgaca cctgcaagca taacacagat cctaagacta gagtgaagta
     7861 ggaagaagaa ttagaaaatc cagtggatgt cctgagtata gggaaccagg gccgttgaaa
     7921 atcagtaaag gttgattacc tggggcgaga ccgggtgact gtggcagtgc aggtgaaggt
     7981 accctggacc ttctcagttc gctggcacat aaggctccgc caataaagcg tggttctctc
     8041 tgtcacacac acacacacac acacacacac acacacacac acacacacac acacaatgat
     8101 tggagggcta tatgatccag cattagcttc ctggtgtgcc aagcatgctt gatcgggaat
     8161 ttttttttat tattattatt ttttagctgt agctgaaggc atttctcgga tgtggagagg
     8221 agaatggaaa tcgcagaacc aaatcagttt gccctgccat atttggctgt ggtctgtcat
     8281 tgggcatttc tgatgtgctt ttctggattc aggaagagct gattgtcctc cgagggttga
     8341 aaaaaaaaaa acagtttcag aaacctgaat ccagggcctt atagttctcc tcattatcta
     8401 tcttcttctc ccttccctcg cccaagggag tgggggaaac acttttcact gcagagtttg
     8461 ctttaaagtt ttcccatctt gcgtgcatta tcccttgata ttaaaattat tttctcagtt
     8521 taatccaact cctgctgaga agctgtgtga gatttaggct gtggggtttt tttcttgtac
     8581 tcttttggat ggtgttgcat ttttcactct taacccgagg gtgtgtttca gcttatgttc
     8641 gttctgtttc atgcaggttt atagcacggt agagtagaag gcggcttctg atttttaggg
     8701 tatttttaga attcattcct gagtgagggg ttcagacacc cagtctcctc ggaacagggg
     8761 tgaggggtcg actgagcttt gttgagaagc ctccagttag gcttcgggcg ggtctccatg
     8821 ttatattgtg tgtttactga gcttcccact ggtagaagat gacacatttg tccatcgtcc
     8881 tgtgtatctg ctttccagag gacaccggag cattctcctg gggtcactcc cacatggctg
     8941 cctcacatag ctgttttgca acagccttta ttgccaacac ccttggaaga acatctcctg
     9001 tagaagtaca agttttgcaa tatggacttt ggaagtggtt ttgtgatgtt cgaattttct
     9061 gttagcctat tgctcaagca ctacagaatg tacatagtcc ctttggccat cctctcaccc
     9121 cagaatatcc taataaacat tgttttcttt tttaaaaaaa a
//
