LOCUS       NM_001244710            8665 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens glutamine--fructose-6-phosphate transaminase 1
            (GFPT1), transcript variant 1, mRNA.
ACCESSION   NM_001244710
VERSION     NM_001244710.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Drury,J., Geisen,M.E., Tessmann,J.W., Rychahou,P.G., Kelson,C.O.,
            He,D., Wang,C., Evers,B.M. and Zaytseva,Y.Y.
  TITLE     Overexpression of Fatty Acid Synthase Upregulates
            Glutamine-Fructose-6-Phosphate Transaminase 1 and O-Linked
            N-Acetylglucosamine Transferase to Increase O-GlcNAc Protein
            Glycosylation and Promote Colorectal Cancer Growth
  JOURNAL   Int J Mol Sci 25 (9), 4883 (2024)
   PUBMED   38732103
  REMARK    GeneRIF: Overexpression of Fatty Acid Synthase Upregulates
            Glutamine-Fructose-6-Phosphate Transaminase 1 and O-Linked
            N-Acetylglucosamine Transferase to Increase O-GlcNAc Protein
            Glycosylation and Promote Colorectal Cancer Growth.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Li,D., Guan,M., Cao,X., Zha,Z.Q., Zhang,P., Xiang,H., Zhou,Y.,
            Peng,Q., Xu,Z., Lu,L. and Liu,G.
  TITLE     GFPT1 promotes the proliferation of cervical cancer via regulating
            the ubiquitination and degradation of PTEN
  JOURNAL   Carcinogenesis 43 (10), 969-979 (2022)
   PUBMED   36040914
  REMARK    GeneRIF: GFPT1 promotes the proliferation of cervical cancer via
            regulating the ubiquitination and degradation of PTEN.
REFERENCE   3  (bases 1 to 8665)
  AUTHORS   Mensch,A., Cordts,I., Scholle,L., Joshi,P.R., Kleeberg,K.,
            Emmer,A., Beck-Woedl,S., Park,J., Haack,T.B.,
            Stoltenburg-Didinger,G., Zierz,S. and Deschauer,M.
  TITLE     GFPT1-Associated Congenital Myasthenic Syndrome Mimicking a
            Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology
            Solved by Next-Generation-Sequencing
  JOURNAL   J Neuromuscul Dis 9 (4), 533-541 (2022)
   PUBMED   35694932
  REMARK    GeneRIF: GFPT1-Associated Congenital Myasthenic Syndrome Mimicking
            a Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology
            Solved by Next-Generation-Sequencing.
REFERENCE   4  (bases 1 to 8665)
  AUTHORS   Nerlich,A.G., Sauer,U., Kolm-Litty,V., Wagner,E., Koch,M. and
            Schleicher,E.D.
  TITLE     Expression of glutamine:fructose-6-phosphate amidotransferase in
            human tissues: evidence for high variability and distinct
            regulation in diabetes
  JOURNAL   Diabetes 47 (2), 170-178 (1998)
   PUBMED   9519709
REFERENCE   5  (bases 1 to 8665)
  AUTHORS   Zhou,J., Neidigh,J.L., Espinosa,R. 3rd, LeBeau,M.M. and
            McClain,D.A.
  TITLE     Human glutamine: fructose-6-phosphate amidotransferase:
            characterization of mRNA and chromosomal assignment to 2p13
  JOURNAL   Hum Genet 96 (1), 99-101 (1995)
   PUBMED   7607664
REFERENCE   6  (bases 1 to 8665)
  AUTHORS   Whitmore,T.E., Mudri,S.L. and McKnight,G.L.
  TITLE     Physical mapping of the human glutamine:fructose-6-phosphate
            amidotransferase gene (GFPT) to chromosome 2p13
  JOURNAL   Genomics 26 (2), 422-423 (1995)
   PUBMED   7601477
REFERENCE   7  (bases 1 to 8665)
  AUTHORS   Sayeski,P.P., Paterson,A.J. and Kudlow,J.E.
  TITLE     The murine glutamine:fructose-6-phosphate amidotransferase-encoding
            cDNA sequence
  JOURNAL   Gene 140 (2), 289-290 (1994)
   PUBMED   8144040
REFERENCE   8  (bases 1 to 8665)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   9  (bases 1 to 8665)
  AUTHORS   McKnight,G.L., Mudri,S.L., Mathewes,S.L., Traxinger,R.R.,
            Marshall,S., Sheppard,P.O. and O'Hara,P.J.
  TITLE     Molecular cloning, cDNA sequence, and bacterial expression of human
            glutamine:fructose-6-phosphate amidotransferase
  JOURNAL   J Biol Chem 267 (35), 25208-25212 (1992)
   PUBMED   1460020
REFERENCE   10 (bases 1 to 8665)
  AUTHORS   Traxinger,R.R. and Marshall,S.
  TITLE     Coordinated regulation of glutamine:fructose-6-phosphate
            amidotransferase activity by insulin, glucose, and glutamine. Role
            of hexosamine biosynthesis in enzyme regulation
  JOURNAL   J Biol Chem 266 (16), 10148-10154 (1991)
   PUBMED   2037571
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI560595.1, DB133199.1,
            AF334737.1, M90516.1 and AC114772.5.
            
            On Sep 19, 2019 this sequence version replaced NM_001244710.1.
            
            Summary: This gene encodes the first and rate-limiting enzyme of
            the hexosamine pathway and controls the flux of glucose into the
            hexosamine pathway. The product of this gene catalyzes the
            formation of glucosamine 6-phosphate. [provided by RefSeq, Sep
            2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.658924.1,
                                           SRR1660807.245495.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357308.9/ ENSP00000349860.4
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-505               BI560595.1         36-540
            506-699             DB133199.1         329-522
            700-981             AF334737.1         175-456
            982-3055            M90516.1           894-2967
            3056-8665           AC114772.5         13808-19417         c
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.3"
     gene            1..8665
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /note="glutamine--fructose-6-phosphate transaminase 1"
                     /db_xref="GeneID:2673"
                     /db_xref="HGNC:HGNC:4241"
                     /db_xref="MIM:138292"
     exon            1..163
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..2256
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /EC_number="2.6.1.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     glucosamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 1; hexosephosphate aminotransferase 1;
                     D-fructose-6-phosphate amidotransferase 1;
                     glutamine:fructose-6-phosphate amidotransferase 1;
                     glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 1"
                     /codon_start=1
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 1 isoform 1"
                     /protein_id="NP_001231639.1"
                     /db_xref="CCDS:CCDS58713.1"
                     /db_xref="GeneID:2673"
                     /db_xref="HGNC:HGNC:4241"
                     /db_xref="MIM:138292"
                     /translation="MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDG
                     GNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEP
                     SPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMY
                     DNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLS
                     TDHIPILYRTARTQIGSKFTRWGSQGERGKDKKGSCNLSRVDSTTCLFPVEEKAVEYY
                     FASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQI
                     MKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIAC
                     GTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMG
                     LRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFAL
                     MMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYH
                     YATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNAL
                     QQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLR
                     GYDVDFPRNLAKSVTVE"
     exon            164..271
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            272..379
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            380..505
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            506..564
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            565..699
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            700..761
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            762..841
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            842..895
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1001
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1165
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1261
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1359
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1480
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1638
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1753
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1881
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..2049
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2211
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..8665
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6731..6736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /note="hexamer: AATAAA"
     polyA_site      6756
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /note="major polyA site"
     regulatory      8648..8653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /note="hexamer: AATAAA"
     polyA_site      8665
                     /gene="GFPT1"
                     /gene_synonym="CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1;
                     GFAT1m; GFPT; GFPT1L; MSLG"
                     /note="major polyA site"
ORIGIN      
        1 gaaggctgtc gttgccttgg ccgtcgcatc cccgagggag tcgtgtcggc gccaccccgg
       61 cccccgagcc cgcagattgc ccaccgaagc tcgtgtgtgc acccccgatc ccgccagcca
      121 ctcgcccctg gcctcgcggg ccgtgtctcc ggcatcatgt gtggtatatt tgcttactta
      181 aactaccatg ttcctcgaac gagacgagaa atcctggaga ccctaatcaa aggccttcag
      241 agactggagt acagaggata tgattctgct ggtgtgggat ttgatggagg caatgataaa
      301 gattgggaag ccaatgcctg caaaatccag cttattaaga agaaaggaaa agttaaggca
      361 ctggatgaag aagttcacaa gcaacaagat atggatttgg atatagaatt tgatgtacac
      421 cttggaatag ctcatacccg ttgggcaaca catggagaac ccagtcctgt caatagccac
      481 ccccagcgct ctgataaaaa taatgaattt atcgttattc acaatggaat catcaccaac
      541 tacaaagact tgaaaaagtt tttggaaagc aaaggctatg acttcgaatc tgaaacagac
      601 acagagacaa ttgccaagct cgttaagtat atgtatgaca atcgggaaag tcaagatacc
      661 agctttacta ccttggtgga gagagttatc caacaattgg aaggtgcttt tgcacttgtg
      721 tttaaaagtg ttcattttcc cgggcaagca gttggcacaa ggcgaggtag ccctctgttg
      781 attggtgtac ggagtgaaca taaactttct actgatcaca ttcctatact ctacagaaca
      841 gctaggactc agattggatc aaaattcaca cggtggggat cacagggaga aagaggcaaa
      901 gacaagaaag gaagctgcaa tctctctcgt gtggacagca caacctgcct tttcccggtg
      961 gaagaaaaag cagtggagta ttactttgct tctgatgcaa gtgctgtcat agaacacacc
     1021 aatcgcgtca tctttctgga agatgatgat gttgcagcag tagtggatgg acgtctttct
     1081 atccatcgaa ttaaacgaac tgcaggagat caccccggac gagctgtgca aacactccag
     1141 atggaactcc agcagatcat gaagggcaac ttcagttcat ttatgcagaa ggaaatattt
     1201 gagcagccag agtctgtcgt gaacacaatg agaggaagag tcaactttga tgactatact
     1261 gtgaatttgg gtggtttgaa ggatcacata aaggagatcc agagatgccg gcgtttgatt
     1321 cttattgctt gtggaacaag ttaccatgct ggtgtagcaa cacgtcaagt tcttgaggag
     1381 ctgactgagt tgcctgtgat ggtggaacta gcaagtgact tcctggacag aaacacacca
     1441 gtctttcgag atgatgtttg ctttttcctt agtcaatcag gtgagacagc agatactttg
     1501 atgggtcttc gttactgtaa ggagagagga gctttaactg tggggatcac aaacacagtt
     1561 ggcagttcca tatcacggga gacagattgt ggagttcata ttaatgctgg tcctgagatt
     1621 ggtgtggcca gtacaaaggc ttataccagc cagtttgtat cccttgtgat gtttgccctt
     1681 atgatgtgtg atgatcggat ctccatgcaa gaaagacgca aagagatcat gcttggattg
     1741 aaacggctgc ctgatttgat taaggaagta ctgagcatgg atgacgaaat tcagaaacta
     1801 gcaacagaac tttatcatca gaagtcagtt ctgataatgg gacgaggcta tcattatgct
     1861 acttgtcttg aaggggcact gaaaatcaaa gaaattactt atatgcactc tgaaggcatc
     1921 cttgctggtg aattgaaaca tggccctctg gctttggtgg ataaattgat gcctgtgatc
     1981 atgatcatca tgagagatca cacttatgcc aagtgtcaga atgctcttca gcaagtggtt
     2041 gctcggcagg ggcggcctgt ggtaatttgt gataaggagg atactgagac cattaagaac
     2101 acaaaaagaa cgatcaaggt gccccactca gtggactgct tgcagggcat tctcagcgtg
     2161 atccctttac agttgctggc tttccacctt gctgtgctga gaggctatga tgttgatttc
     2221 ccacggaatc ttgccaaatc tgtgactgta gagtgaggaa tatctataca aaatgtacga
     2281 aactgtatga ttaagcaaca caagacacct tttgtattta aaaccttgat ttaaaatatc
     2341 accccttgaa gccttttttt agtaaatcct tatttatata tcagttataa ttattccact
     2401 caatatgtga tttttgtgaa gttacctctt acattttccc agtaatttgt ggaggacttt
     2461 gaataatgga atctatattg gaatctgtat cagaaagatt ctagctatta ttttctttaa
     2521 agaatgctgg gtgttgcatt tctggaccct ccacttcaat ctgagaagac aatatgtttc
     2581 taaaaattgg tacttgtttc accatacttc attcagacca gtgaaagagt agtgcattta
     2641 attggagtat ctaaagccag tggcagtgta tgctcatact tggacagtta gggaagggtt
     2701 tgccaagttt taagagaaga tgtgatttat tttgaaattt gtttctgttt tgtttttaaa
     2761 tcaaactgta aaacttaaaa ctgaaaaatt ttattggtag gatttatatc taagtttggt
     2821 tagccttagt ttctcagact tgttgtctat tatctgtagg tggaagaaat ttaggaagcg
     2881 aaatattaca gtagtgcatt ggtgggtctc aatccttaac atatttgcac aattttatag
     2941 cacaaacttt aaattcaagc tgctttggac aactgacaat atgattttaa atttgaagat
     3001 gggatgtgta catgttgggt atcctactac tttgtgtttt catctcctaa aagtggtttt
     3061 tatttccttg tatctgtagt cttttatttt ttaaatgact gctgaatgac atattttatc
     3121 ttgttcttta aaatcacaac acagagctgc tattaaatta atattgatat attcagtatt
     3181 ctcttcaact ttgtcacgag gaagaatttc ctgtagttaa ttttaacttt ccttcattac
     3241 attgttgcat aaaactagtc ccttagtgcc agtttggaag ttacttgcaa ttgtttggaa
     3301 gatttgcagg tcgctgacct catcatttca ctctaagtat atgtggtagg ctaagctcag
     3361 aaaaaggtat gtatgcttta cactgatagg caccaaattt agtattgtat cctaagtatt
     3421 tttcctcttc tgtattttct gtgctctacc cctgaaatat atttaggaat aaagaagata
     3481 taaataaagt gatattgggg tatgttcagc ttgtaagtgt cagaaatgga agttgacaat
     3541 ttggattaaa atatttaaag tagaatgtta ttatttgata cccccaaaat tgtgagtaat
     3601 aatttcttaa tggagctttt ctggccagat cttcagggct ataggagagt gtgtttgttt
     3661 ttggtgaagt ccttcttttt caaaggtttt tacttttaaa ttgtgaagat aagctattta
     3721 gcacaattat ttaagtaagc tgttttttcc tttcttttct ttcttttctt gttttaactt
     3781 ctgggtgagg ttaagatttt ctaatagtta ttgttgttgc cagagaagca tagaattctg
     3841 cttgtgtctt agggttagag aaagatttgt tatattttga ggtaatttgg aaggactttc
     3901 atacatggtg actagaaacc tgatcttttt aagaataaaa tagggtcact atttacatta
     3961 tatataatat atggctatta tagctatatt aatctacttt aattgtttct aagattattt
     4021 tgtgtaagtc agctagcgtg tgtatagtgt catttcttat gttttgtata ttaacgtttt
     4081 acaaacacaa tttgtgtggg tttgtagcat ttcttatagt ttaaagtatg attcagcatt
     4141 ctaagttaaa actaatatgt gaagtcagtc attagtctgc ataatctctg tctccctctg
     4201 tgtgtgtctt tctataaagc acatgtgtac acacacacac acaaatatac tgaaagctag
     4261 ggtaagttct aaactgaata tcaaaaacca aaatcaagaa caaagaagtg atattttcca
     4321 aacaaacatg gattctctgc cgggtgcagt ggctcagcct gtaataccag cctccacttt
     4381 gggagtctga ggtgggagta ttgcttgagc tcaagagttg gagaacagcc tgggcaacat
     4441 ggtgagacct ggtctttaca aaaatttttt tggccggacg cagtggctca cacctgtaat
     4501 cccagcactt tgggaggccg aggcaggttg atcatgaggt caagagatcg agaccatcct
     4561 ggccaacatg gtgaaacccc gtctttacta aaaatacaaa aattagctgg gagtggtggc
     4621 gtgcgcctgt agtcccaact acttgggagg ctgaggcagg agaatcgctt gaacccagga
     4681 ggcggaggtt gcagtgaggc gagatcacgc cactacactc cagcctggcg acagagcaag
     4741 actctctcaa aaaaaaaaaa aaaaaatttt ttttttaaat gggctgggca tggtggcttg
     4801 tgcctgtagt ctcagcaact tgggaggctt aagtagtagg atcgcttgag cctgggaggt
     4861 tgaggctgca gtgagctgtg attgtgccac tgtgcttcag cttgagtgac agagtgagac
     4921 cctcctgact caaaacaaaa aacatggttt ctcctctctc tcctgtcttc ttactctcta
     4981 tcccatttga tgtagtgatt tttaaatgct tttgtaagtt aattcttaac acaaaagaga
     5041 cattgtaatg aggcacacca ctaaagtgag catgcccaat taaaaccagt gtaatatagg
     5101 ataagaaaat ctgatttttc aaaaaagata ctctacataa agaatccttc atataaaaag
     5161 ttctttcttg tagtacattt aaagttttaa ttcactcatg tataactgag agttcctttg
     5221 agcccttttt aggcagggag gcatgtctgt catctagcgt gtggcccagt aagtgattat
     5281 tacattggaa tcagtttttc agtcttttaa aataaattct gtgccataag aataaaagat
     5341 aaagagaaaa attaatgtta actattttta gcttattata actatgtcaa caagtgttta
     5401 ttaataccta ttatgggaaa gtcactgtgg ttggcattga aaattacatc atctttaaag
     5461 cagtatttgt ccccagatgg actcatcact agcaaagact aggttcattg gaaggcatag
     5521 ggtgagagaa tgggaagatg aagtggaggc gggttgttaa agtgctgtca gtgagtgatt
     5581 ttgtctactt gaataatggt ccatgtttgg gggcatattg tgtttcataa gaagtgaaag
     5641 gtatttgcaa agtaagctac aaatgaccca taaatctgtt aacaacagtc cttaatatgc
     5701 aaagatgaaa aacaagcatt actgctaccc aaagggaact ggtgcttggt gatgtgcaga
     5761 tggggctgtt ggttaagaga gctattacag gttttctctc ttaggtttca taggaggtag
     5821 ttactgagat gagattgttt tatctttttg aatacagatc tcttgtcttg agttagttct
     5881 gaggatggga gtaataaagg agttttttgt ttttttgttt gtttgtttgt tttggctcct
     5941 tagtaatact cctctgacat ttatttctat tattcttcaa agaaaggaaa ccaactgaaa
     6001 tgtttgcttt aacaaacatt ttaataagtt ctctgggttt ttttttcccc ttttaaaaaa
     6061 attagcatat accatagcaa taaaagaact aatgttaact attgtatgct acaacttaag
     6121 tgatttttct aaagaagcac aatgtcattg aaagtattat tgaaaaggat catagtcaca
     6181 ttgaatttgt gaaggccaaa gaaattgaag ggagtgatat tttcatttta tgatattcac
     6241 atatttagta aattttgtgt acaagaatac caggcagagt gttttaccca tggaaacagg
     6301 tttcagatta ctttattttt actgttagag tctcaagttt agaaatgcta acacttaaat
     6361 cagttttttt ctcactatac ttgaagattg ttaatatttt gatatcttcc tagcttgatg
     6421 aatttaaaca tatcttcaga tctgtgacag tgacagccaa taggactgat aatattagct
     6481 tcaaaccaat aatatccagg gttaaaataa aaatcatagt gaaagtacga ttgtaaaatt
     6541 atgctatatt aacttttaag tctgtaataa cttgacatca aaatgttatg taattaccat
     6601 aaataatggc tagcgagaac atctttggaa attctcaaat tacctttctt actacactgt
     6661 ttgcagaatg aatgtagaaa tgatcctgtt agctttctga atgttctgtg gttgaatgtg
     6721 tttttgctta aataaagctt ttggtatttg tttaaattac acttcttgag aagtggaaat
     6781 tttaggatca tctttgcttt gtttcagttt tgtgattttt gaaatgaatg tttagtttac
     6841 tgagccagtt ggtcatttct tcctcatgtc gttaagtcca gtgagtaagc ctgaactgtg
     6901 aataaattac caaaaacttg cttagaattt cattttgaag caatttgcta atatttgaag
     6961 tgtatacaca tttgtagtta tgttagaaat tgtattgtac taagaatgta atcaatgtct
     7021 actttagttg taaacatttc tgatgtcaaa actttattca ttactgttga ttttaagaat
     7081 aagaaatcac tgcctaaata ttaccaaaag ccactgtctc tacccgaact tcccagtttg
     7141 ggaaagaatc gttagataaa acaaaggctc tgccctttct gataccaaac tccacagata
     7201 ctttctcaca tcttttaaaa cattttgcaa taacatattg tttataggaa gtttacgggg
     7261 tatgcaatga ttaaaacttt taagtgaatt gatagttgca aaagaaagga taatatttaa
     7321 ggtcagtgag tagcaagaca atctaaagtt tctgtaatat ctggctctct gttaattata
     7381 gagcaaagtt tcccttacag aatcctttta tgaacagcaa gctagagtct atccctagtg
     7441 gttatagcac ctgctgcgtt tttcaggaga cagttaggcc agggtgattt gaatggatag
     7501 atgtgctgtt ttgcctgctt gtagaattca gcccagtctt tggtctctct ctctctctcc
     7561 ctcctccacc tctccctctc tcttcttctg caccagagcc taaggctgcg ccaccaagat
     7621 gcgtcatttt ttccagaggc ttcttttttt tttttttgag atggagtttt gctctgttgc
     7681 ccaggctggc atgcagtgct gcaatcttgg ctgactgcaa cttccacctc ccgtgttcac
     7741 acgagtcttt tgcctcagcc tctggattag ctgggattac aggcacgtgc caccatgcct
     7801 agctaatttt tgtgttttta gtagagacgg ggtttcacca tgttggccag gctagtctca
     7861 aactcctgac cttaagtgat ctgcccgcct tggcctccca aagtgctggg attacaggca
     7921 tgagtcaccg tgtcctgccc agacatatca aatttgacag gtattgtata ccctttggat
     7981 ctttaggaat taatttttgc ctctgtcact cagctttgta tattttgaaa tggagataag
     8041 tatagggagg tcttggaagg aaaattgcca gaattcccaa accatgtaac actcattgag
     8101 aattccagat ccattatatc taaagggcaa gtgaaggaaa cagtattgtg aactgggtat
     8161 aactccttgg ttcttaacta gtacattctt aatctgtgag acccaaaggt tgataaacaa
     8221 taatttaaga ttgtacagta ctctaaacgt ctgcaaaggt ctagatgtta tcagtatcac
     8281 tagtttttat ttctgccagt agctcccttt taggttacat tgttgtcctc tttccagtgt
     8341 cgcatctgtc attggttttt cactatggca agttcattaa aaagcttgct ccattgttat
     8401 cttcaagtaa tgcccataag gagatggaag atatctgaga caattaaggc tttagcttct
     8461 aggcaagaga aataacgttg cattaaattt caagtttctt tctgctagac ttgaatgtgt
     8521 ctagccactc taatttatgg gggcttttgg ttttttccta ttgtactttg tatgtagaat
     8581 tgttttgaaa tatcaagcat atttactttg aatttgaact ctttcttaat tttgtattta
     8641 tcctttgaat aaaatgtaaa tccaa
//
