LOCUS       NM_024996               6478 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens G elongation factor mitochondrial 1 (GFM1), transcript
            variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_024996
VERSION     NM_024996.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Khan,A.U., Khan,I., Khan,M.I., Latif,M., Siddiqui,M.I., Khan,S.U.,
            Htar,T.T., Wahid,G., Ullah,I., Bibi,F., Khan,A., Naseer,M.I.,
            Seo,G.H. and Jelani,M.
  TITLE     Whole exome sequencing identifies a novel compound heterozygous
            GFM1 variant underlying developmental delay, dystonia,
            polymicrogyria, and severe intellectual disability in a Pakhtun
            family
  JOURNAL   Am J Med Genet A 188 (9), 2693-2700 (2022)
   PUBMED   35703069
  REMARK    GeneRIF: Whole exome sequencing identifies a novel compound
            heterozygous GFM1 variant underlying developmental delay, dystonia,
            polymicrogyria, and severe intellectual disability in a Pakhtun
            family.
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 6478)
  AUTHORS   Shen,Y., Yan,K., Dong,M., Yang,R. and Huang,X.
  TITLE     [Analysis of GFM1 gene mutations in a family with combined
            oxidative phosphorylation deficiency 1]
  JOURNAL   Zhejiang Da Xue Xue Bao Yi Xue Ban 49 (5), 574-580 (2020)
   PUBMED   33210482
  REMARK    GeneRIF: [Analysis of GFM1 gene mutations in a family with combined
            oxidative phosphorylation deficiency 1].
REFERENCE   4  (bases 1 to 6478)
  AUTHORS   Kummer,E. and Ban,N.
  TITLE     Structural insights into mammalian mitochondrial translation
            elongation catalyzed by mtEFG1
  JOURNAL   EMBO J 39 (15), e104820 (2020)
   PUBMED   32602580
  REMARK    GeneRIF: Structural insights into mammalian mitochondrial
            translation elongation catalyzed by mtEFG1.
REFERENCE   5  (bases 1 to 6478)
  AUTHORS   Koripella,R.K., Sharma,M.R., Bhargava,K., Datta,P.P., Kaushal,P.S.,
            Keshavan,P., Spremulli,L.L., Banavali,N.K. and Agrawal,R.K.
  TITLE     Structures of the human mitochondrial ribosome bound to EF-G1
            reveal distinct features of mitochondrial translation elongation
  JOURNAL   Nat Commun 11 (1), 3830 (2020)
   PUBMED   32737313
  REMARK    GeneRIF: Structures of the human mitochondrial ribosome bound to
            EF-G1 reveal distinct features of mitochondrial translation
            elongation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6478)
  AUTHORS   Bhargava,K., Templeton,P. and Spremulli,L.L.
  TITLE     Expression and characterization of isoform 1 of human mitochondrial
            elongation factor G
  JOURNAL   Protein Expr Purif 37 (2), 368-376 (2004)
   PUBMED   15358359
  REMARK    GeneRIF: analysis of molecular model for EF-G1 isoform
REFERENCE   7  (bases 1 to 6478)
  AUTHORS   Hammarsund,M., Wilson,W., Corcoran,M., Merup,M., Einhorn,S.,
            Grander,D. and Sangfelt,O.
  TITLE     Identification and characterization of two novel human
            mitochondrial elongation factor genes, hEFG2 and hEFG1,
            phylogenetically conserved through evolution
  JOURNAL   Hum Genet 109 (5), 542-550 (2001)
   PUBMED   11735030
REFERENCE   8  (bases 1 to 6478)
  AUTHORS   Gao,J., Yu,L., Zhang,P., Jiang,J., Chen,J., Peng,J., Wei,Y. and
            Zhao,S.
  TITLE     Cloning and characterization of human and mouse mitochondrial
            elongation factor G, GFM and Gfm, and mapping of GFM to human
            chromosome 3q25.1-q26.2
  JOURNAL   Genomics 74 (1), 109-114 (2001)
   PUBMED   11374907
REFERENCE   9  (bases 1 to 6478)
  AUTHORS   Bec,G., Kerjan,P., Zha,X.D. and Waller,J.P.
  TITLE     Valyl-tRNA synthetase from rabbit liver. I. Purification as a
            heterotypic complex in association with elongation factor 1
  JOURNAL   J Biol Chem 264 (35), 21131-21137 (1989)
   PUBMED   2556394
REFERENCE   10 (bases 1 to 6478)
  AUTHORS   Motorin,Yu.A., Wolfson,A.D., Orlovsky,A.F. and Gladilin,K.L.
  TITLE     Mammalian valyl-tRNA synthetase forms a complex with the first
            elongation factor
  JOURNAL   FEBS Lett 238 (2), 262-264 (1988)
   PUBMED   3169261
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025033.20 and AC080013.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 3, 2019 this sequence version replaced NM_024996.6.
            
            Summary: Eukaryotes contain two protein translational systems, one
            in the cytoplasm and one in the mitochondria. Mitochondrial
            translation is crucial for maintaining mitochondrial function and
            mutations in this system lead to a breakdown in the respiratory
            chain-oxidative phosphorylation system and to impaired maintenance
            of mitochondrial DNA. This gene encodes one of the mitochondrial
            translation elongation factors. Its role in the regulation of
            normal mitochondrial function and in different disease states
            attributed to mitochondrial dysfunction is not known. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.24161.1,
                                           SRR18074969.1549526.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000486715.6/
                                                  ENSP00000419038.1
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-189               AC025033.20        23396-23584         c
            190-342             AC025033.20        22330-22482         c
            343-475             AC025033.20        21814-21946         c
            476-680             AC025033.20        21164-21368         c
            681-797             AC025033.20        18954-19070         c
            798-948             AC025033.20        15865-16015         c
            949-1106            AC025033.20        14644-14801         c
            1107-1191           AC025033.20        13480-13564         c
            1192-1329           AC025033.20        9052-9189           c
            1330-1431           AC025033.20        7136-7237           c
            1432-1488           AC025033.20        5427-5483           c
            1489-1626           AC025033.20        2637-2774           c
            1627-1709           AC025033.20        1725-1807           c
            1710-1872           AC080013.17        15889-16051
            1873-2017           AC080013.17        18418-18562
            2018-2178           AC080013.17        24057-24217
            2179-2232           AC080013.17        25033-25086
            2233-6478           AC080013.17        25230-29475
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.32"
     gene            1..6478
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="G elongation factor mitochondrial 1"
                     /db_xref="GeneID:85476"
                     /db_xref="HGNC:HGNC:13780"
                     /db_xref="MIM:606639"
     exon            1..189
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..2364
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mitochondrial elongation factor G1; G translation
                     elongation factor, mitochondrial"
                     /codon_start=1
                     /product="elongation factor G, mitochondrial isoform 2"
                     /protein_id="NP_079272.4"
                     /db_xref="CCDS:CCDS33885.1"
                     /db_xref="GeneID:85476"
                     /db_xref="HGNC:HGNC:13780"
                     /db_xref="MIM:606639"
                     /translation="MRLLGAAAVAALGRGRAPASLGWQRKQVNWKACRWSSSGVIPNE
                     KIRNIGISAHIDSGKTTLTERVLYYTGRIAKMHEVKGKDGVGAVMDSMELERQRGITI
                     QSAATYTMWKDVNINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVNR
                     QMKRYNVPFLTFINKLDRMGSNPARALQQMRSKLNHNAAFMQIPMGLEGNFKGIVDLI
                     EERAIYFDGDFGQIVRYGEIPAELRAAATDHRQELIECVANSDEQLGEMFLEEKIPSI
                     SDLKLAIRRATLKRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNKEDD
                     SKEKTKILMNSSRDNSHPFVGLAFKLEVGRFGQLTYVRSYQGELKKGDTIYNTRTRKK
                     VRLQRLARMHADMMEDVEEVYAGDICALFGIDCASGDTFTDKANSGLSMESIHVPDPV
                     ISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRL
                     EREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTK
                     LEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEI
                     SFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVE
                     DYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATG
                     QLPVKKGKAKN"
     exon            190..342
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            343..475
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            476..680
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            681..797
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            798..948
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1106
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1191
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1329
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1431
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1488
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1626
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1709
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1872
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2017
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2178
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2232
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..6478
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3014..3019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="hexamer: ATTAAA"
     polyA_site      3035
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="major polyA site"
     regulatory      3449..3454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="hexamer: AATAAA"
     polyA_site      3469
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="major polyA site"
     regulatory      6444..6449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
                     /note="hexamer: AATAAA"
     polyA_site      6478
                     /gene="GFM1"
                     /gene_synonym="COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1;
                     mtEF-G1"
ORIGIN      
        1 gctcttacaa cattggctgc cggcgtgact ttgaccgctt cccggtgcgt taccggcagc
       61 tgaacccacc cggcgccacg ggactttgac gcgtgctctg cgcttgccat gagactcctg
      121 ggagctgcag ccgtcgcggc tctggggcgc ggaagggccc ccgcctccct aggctggcag
      181 aggaagcagg ttaattggaa ggcctgccga tggtcttcat caggggtgat tcctaatgaa
      241 aaaatacgaa atattggaat ctcagctcac attgattctg ggaaaactac attaacagaa
      301 cgagtccttt actacactgg cagaattgca aagatgcatg aggtgaaagg taaagatgga
      361 gttggtgctg tcatggattc catggaacta gagagacaaa gaggaatcac tattcagtca
      421 gcagccactt acaccatgtg gaaagatgtc aatattaaca ttatagatac tcctgggcat
      481 gtggacttca caatagaagt ggaaagggcc ctgagagtgt tggatggtgc agtccttgtt
      541 ctctgtgctg ttggaggggt acagtgccag accatgactg tcaatcgtca gatgaagcgc
      601 tacaacgttc cgtttctaac ttttattaac aaattggacc gaatgggctc caacccagcc
      661 agggccctgc agcaaatgag gtctaaacta aatcataatg cagcgtttat gcagataccc
      721 atgggtttgg agggtaattt taaaggtatt gtagatctta ttgaggaacg agccatctat
      781 tttgatggag actttggtca gattgttcga tatggtgaga ttccagctga attaagggcg
      841 gcggccactg accaccggca ggagctaatt gaatgtgttg ccaattcaga tgaacagctt
      901 ggtgagatgt ttctggaaga aaaaatcccc tcgatttctg atttaaagct agcaattcga
      961 agagctactc tgaaaagatc atttactcct gtatttttgg gaagcgcctt gaagaacaaa
     1021 ggagttcagc ctcttttaga tgctgtttta gaatacctcc caaatccatc tgaagtccag
     1081 aactatgcta ttctcaataa agaggatgac tcaaaagaga aaaccaaaat cctaatgaac
     1141 tccagtagag acaattccca cccatttgta ggcctggctt ttaaactgga ggtaggtcga
     1201 tttggacaat taacttatgt tcgcagttat cagggagagc taaagaaggg tgacaccatc
     1261 tataacacaa ggacaagaaa gaaagtacgg ttgcaacggc tggctcgcat gcatgccgac
     1321 atgatggagg atgttgagga agtatatgcc ggagacatct gtgcattgtt tggcattgac
     1381 tgtgctagtg gagacacatt cacagacaaa gccaacagcg gcctttctat ggagtcaatt
     1441 catgttcctg atcctgtcat ttcaatagca atgaagcctt ctaacaagaa cgatctggaa
     1501 aaattttcaa aaggtattgg caggtttaca agagaagatc ccacatttaa agtatacttt
     1561 gacactgaga acaaagagac agttatatct ggaatgggag aattacacct ggaaatctat
     1621 gctcagaggc tggaaagaga gtatggctgt ccttgtatca caggaaagcc aaaagttgcc
     1681 tttcgagaga ccattactgc ccctgtcccg tttgacttta cacataaaaa acaatcaggt
     1741 ggtgcaggcc agtatggaaa agtaataggt gtcctggagc ctctggaccc agaggactac
     1801 actaaattgg aattttcaga tgaaacattc ggatcaaata ttccaaagca gtttgtgcct
     1861 gctgtagaaa aggggttttt agatgcctgc gagaagggcc ctctttctgg tcacaagctc
     1921 tctgggctcc ggtttgtcct gcaagatgga gcacaccaca tggttgattc taatgaaatc
     1981 tctttcatcc gagcaggaga aggtgctctt aaacaagcct tggcaaatgc aacattatgt
     2041 attcttgaac ctattatggc tgtggaagtt gtagctccaa atgaatttca gggacaagta
     2101 attgcaggaa ttaaccgacg ccatggggta atcactgggc aagatggagt tgaggactat
     2161 tttacactgt atgcagatgt ccctctaaat gatatgtttg gttattccac tgaacttagg
     2221 tcatgcacag agggaaaggg agaatacaca atggagtata gcaggtatca gccatgttta
     2281 ccatccacac aagaagacgt cattaataag tatttggaag ctacaggtca acttcctgtt
     2341 aaaaaaggaa aagccaagaa ctaactttgc ttactgtgag ttgactgact ctaattgaat
     2401 ctgcgtggtt ttgatacttt gatggattcc agtggaataa attcaggctg ctgaaacaag
     2461 aaattctgag cccaggaagc gggctcttct ttcttcaaaa gaagcccttc ttgttcatat
     2521 tcaggagctt ctgttatatt caaaggtaat tctatgtcta tctcaactct attgattggt
     2581 tttatagttc attgaaaatc ctcaaataaa atataattat tactgaaata tgtttaatat
     2641 ttaaggggaa aagagactaa tttcagttat acttttaagc ttagaatgta tgttcatttc
     2701 caaattttgt atcataagag ttttcaacat agagaaaagc tgaaaaaatg caaagaataa
     2761 ccacatactt tccatctacc ttcctttgtt aacgggttgt ttatcatata ataatttgtt
     2821 ttgtcatatt tgctttcact gtctattatc tgtttaagtc tcataactct atttttagtt
     2881 tgctgaagac ttgaaagtga atcgcatata tcatgacact tcttggagtg tcattaatgg
     2941 gcaggctttt ctgttgaaga gtggattccg tatgttcttc atagagagtg tttttcagat
     3001 tcttcattgg gatattaaaa tattagccaa atttctctct gttttatata tgtctgttta
     3061 tttcagtttg tggtttctgc aaatttgtaa ctgcctctgt tttaggagta taagtattac
     3121 ttccttgtgg tctattgtga agtaaaaagt agacccttgc atatactatt cttgtttgtg
     3181 ttcatcttaa tgtttttgta cagctaaatc aaatgtaatt tatagagtta gtttcatcaa
     3241 cctaatgaat gctagttaaa tttgaattcc ttggaattta tcgtatattg tattcactga
     3301 gattatgaag ggacaaatgt taatcttttg tttccagaaa aagttgggct ttcccaagca
     3361 gttctattac ccggttcaga attgcttcat ccaaaaatca tctgatggta tagatggatc
     3421 ctagtccttt tcattacctg atggtagaaa taaaataatt gattttaaat tcctttgctt
     3481 ttcttgactt atagacgtgg ctttatactt taaggtttct aggggaaaaa gatctttaaa
     3541 aagtattgag cacatgtttt caaggcattt gctaaatact tttttttttt taatttttat
     3601 ttttttgtgg tagggtctca ctctatcccc caggctggaa tgcagaggga ctatctctgc
     3661 tcactacaac ctctgcttcc tgggttcaag caattatccc atctcagctg cctaagtagg
     3721 tgggactaca ggtgtgcgcc accactccct gctaattttt tgcatttttt gaagagatag
     3781 ggttttgcca tattgcccag gctggtctca aactcctggg ctcaagctct ctgcctgccc
     3841 cagcctccca aagtgctggg attatgggca tgaaccacca tgcctggctg ctaaatactt
     3901 taaatgcatt gtataatttc attctcacaa ccccgagagt aagtactatt attatgtcca
     3961 ttttgtaata gaagaagcac agataaggta aataacttta atgagaccac aaaacctaag
     4021 ttttttaatt gatactttaa ttacaattat tcaccaagtg catttccccc atgaatgtct
     4081 gttgctacag tagcatcagg tctgaaaaaa tgtggttgct ttggcatttc ctcagcatct
     4141 tgtgacattc acaataactt tgaatattgt tcctaatgag gaaatacatt ctgtgtacct
     4201 tcatggaatt tagcatgatt cctatggcta ttgagaatag tcacaactac tggaactaaa
     4261 ctggataaat aagtaaaaaa cttacttatt tttaatttgt aaaacatcct ggttataatg
     4321 ttacagtgtt agaaaaggtc ctagatattt aatccaacac cagcatttca taggtaatga
     4381 ttcaggccta gctggtggga gtaattagaa taggttcaca aatggacaga tcaggactag
     4441 aacccacatc tctttctaag tcattagtta tagttggcaa agatcacata ccaaccaaag
     4501 tctgtagcat ggaaaaaaat taatgagaat attcataggt gagggttgtt tatactacta
     4561 tcaagaagga taattgttgg ctaattcaaa tattacatcc ttgtattgga caacagtagc
     4621 agcactaggt agtattgtac tgattcccaa ccctcactgt actttaaaaa tcactggggg
     4681 agccttaaaa agaaatacct aggcctacac ccagatattt agattatttg gctggatcaa
     4741 gataaaaact tttttgggtg attctaatat gcagacaaag ttaaagaccg ctgaaatatt
     4801 gcctgcactg cgcttaagcc caagacatga aatgaaaaat ctgtgaaatt caggagtgtc
     4861 atattgtatt cttttgtgtg atattagagt ttggtttttt tgtttaacaa ctgttttttt
     4921 ttttaagaga tggggtcttc taagacaaaa accatctgac tcagcaatac cattactata
     4981 tacccaaagg aacgtaaatc attctattat aaagacacat gcatgcacac attcattgca
     5041 gaactgttca taatggcaaa gatatggaat caacctaaat gcctatcact gatagactgg
     5101 ataaagaaaa tgtggtacat acacattgtg gaatactatg cagccataaa aaaagaatga
     5161 gatcaagtcc tttgcaggga catagatgga gctggaagcc attatcctca tcaaactaac
     5221 ataggaaaag aaaaccaaat actgcatgtt ctcacttata agtgggagct aaataatgag
     5281 aacacatgga cacacagagg ggaacaacac acactggggc ctattggagg atggagggtg
     5341 ggtggaggga gaggatcagg aaaaataact aatgggtact aggcttaata ccttgatgat
     5401 gaaataatct gtacaacaaa cccccaggac acaagtttag ctatgtaaca agcctgcaca
     5461 tcaacccctg aacttaaaga gatggggtct cgctgtgttt cccaggctgc tcacaaattc
     5521 atgagctcaa gcagtcctct ggcctcagcc tctggagtag ctgggacaag aggcacacac
     5581 caccatgcct ggcttgataa ctttacctta aaaaaaaaag tgttcctatt tatataagct
     5641 tccaactgac tttcaaaaga acttgttcaa cttctaaaaa ttcgtaattc aggtaggaag
     5701 aaaatgaaag tatgaataaa ataaaatgga atgtagacaa aatccctggt attattttat
     5761 atgtcttaag aaagttctaa aggtattagg aaaatgaatg tcaagctgta tgtaattgta
     5821 attagctaag agtgattcag aataatttag actgttaatg aaaagaggac atagggaata
     5881 gtttttcatt ttatgccaag tgatttttct gttttattag ctagtgaaac caatgttggt
     5941 aaaattaagt taacaatttt agagtttgtg ctagaaaaga tattctgagg ccggatgtgg
     6001 tggctcacgc ctgtaattcc agcactttgg gaggccaagg tgggtggata acctgaggtc
     6061 aggagttcga gaccaccctg gccaacacgg tgaaaccccg tctctactat aaatacaaaa
     6121 atcgtccggg cggggtggct cacgcctgta gccccagctg ctcaggaggc tgaggcagga
     6181 gggtcgcttg aacccggaag gcggaggcta tagtgagctg agatggcacc actgcactcc
     6241 agcactccag cactccagcc taggcaacag agtgagactc tgtctcaaaa aaaaaagaaa
     6301 aaaaagaaaa gatgttctga tggtgatact gataaatttt tagttggttt aactttttgg
     6361 gttttgtaaa ttaataaatt aggtaattgt gtttgtatat tatttgtatg tatagaattc
     6421 cccctcataa tctattgtac tgtaataaat ctgctaaaag gttaccttag aaacatta
//
