LOCUS       NM_001130009            9957 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens GEN1 Holliday junction 5' flap endonuclease (GEN1),
            transcript variant 2, mRNA.
ACCESSION   NM_001130009
VERSION     NM_001130009.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Du,X., Wang,C., Liu,J., Yu,M., Ju,H., Xue,S., Li,Y., Liu,J.,
            Dai,R., Chen,J., Zhai,Y., Rao,J., Wang,X., Sun,Y., Sun,L., Wu,X.,
            Xu,H. and Shen,Q.
  TITLE     GEN1 as a risk factor for human congenital anomalies of the kidney
            and urinary tract
  JOURNAL   Hum Genomics 18 (1), 41 (2024)
   PUBMED   38654324
  REMARK    GeneRIF: GEN1 as a risk factor for human congenital anomalies of
            the kidney and urinary tract.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   Kim,J., Yu,J., Lee,S., Kim,S., Lee,I.S., Lee,W. and Chun,S.
  TITLE     Reverse Transcription-PCR-based Sanger Sequencing-confirmed
            Exon-skipping Effect of a Novel GEN1 Intronic Variant (c.1408+4A>G)
  JOURNAL   Ann Lab Med 44 (2), 188-191 (2024)
   PUBMED   37840312
  REMARK    GeneRIF: Reverse Transcription-PCR-based Sanger
            Sequencing-confirmed Exon-skipping Effect of a Novel GEN1 Intronic
            Variant (c.1408+4A>G).
REFERENCE   3  (bases 1 to 9957)
  AUTHORS   Kim,J.H., Youn,Y. and Hwang,J.H.
  TITLE     NCAPH Stabilizes GEN1 in Chromatin to Resolve Ultra-Fine DNA
            Bridges and Maintain Chromosome Stability
  JOURNAL   Mol Cells 45 (11), 792-805 (2022)
   PUBMED   36380731
  REMARK    GeneRIF: NCAPH Stabilizes GEN1 in Chromatin to Resolve Ultra-Fine
            DNA Bridges and Maintain Chromosome Stability.
REFERENCE   4  (bases 1 to 9957)
  AUTHORS   Chan,Y.W. and West,S.
  TITLE     GEN1 promotes Holliday junction resolution by a coordinated nick
            and counter-nick mechanism
  JOURNAL   Nucleic Acids Res 43 (22), 10882-10892 (2015)
   PUBMED   26578604
  REMARK    GeneRIF: human GEN1 protein promotes Holliday junction resolution
            by a mechanism that is analogous to that exhibited by the
            prototypic HJ resolvase E. coli RuvC.
REFERENCE   5  (bases 1 to 9957)
  AUTHORS   Chan,Y.W. and West,S.C.
  TITLE     Spatial control of the GEN1 Holliday junction resolvase ensures
            genome stability
  JOURNAL   Nat Commun 5, 4844 (2014)
   PUBMED   25209024
  REMARK    GeneRIF: GEN1 is controlled by nuclear exclusion, driven by a
            nuclear export signal that restricts GEN1 actions to mitosis.
            Spatial control of GEN1 contributes to genome stability by avoiding
            competition with non-crossover promoting repair pathways.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9957)
  AUTHORS   Garner,E., Kim,Y., Lach,F.P., Kottemann,M.C. and Smogorzewska,A.
  TITLE     Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are
            essential for the resolution of replication-induced Holliday
            junctions
  JOURNAL   Cell Rep 5 (1), 207-215 (2013)
   PUBMED   24080495
  REMARK    GeneRIF: GEN1 activity cannot be substituted for the
            SLX4-associated nucleases, and one of the HJ resolvase activities,
            either of those associated with SLX4 or with GEN1, is required for
            cell viability, even in the presence of BLM.
REFERENCE   7  (bases 1 to 9957)
  AUTHORS   Gao,M., Rendtlew Danielsen,J., Wei,L.Z., Zhou,D.P., Xu,Q., Li,M.M.,
            Wang,Z.Q., Tong,W.M. and Yang,Y.G.
  TITLE     A novel role of human holliday junction resolvase GEN1 in the
            maintenance of centrosome integrity
  JOURNAL   PLoS One 7 (11), e49687 (2012)
   PUBMED   23166748
  REMARK    GeneRIF: Our findings provide novel insight into the biological
            functions of GEN1 by uncovering an important role of GEN1 in the
            regulation of centrosome integrity.
REFERENCE   8  (bases 1 to 9957)
  AUTHORS   Lorenz,A., West,S.C. and Whitby,M.C.
  TITLE     The human Holliday junction resolvase GEN1 rescues the meiotic
            phenotype of a Schizosaccharomyces pombe mus81 mutant
  JOURNAL   Nucleic Acids Res 38 (6), 1866-1873 (2010)
   PUBMED   20040574
  REMARK    GeneRIF: ectopic expression of GEN1 in fission yeast mus81Delta
            strains results in Holliday junction resolution and crossover
            formation during meiosis.
REFERENCE   9  (bases 1 to 9957)
  AUTHORS   Ip,S.C., Rass,U., Blanco,M.G., Flynn,H.R., Skehel,J.M. and
            West,S.C.
  TITLE     Identification of Holliday junction resolvases from humans and
            yeast
  JOURNAL   Nature 456 (7220), 357-361 (2008)
   PUBMED   19020614
  REMARK    GeneRIF: Recombinant GEN1 and Yen1 resolve Holliday junctions by
            the introduction of symmetrically related cuts across the junction
            point, to produce nicked duplex products in which the nicks can be
            readily ligated
REFERENCE   10 (bases 1 to 9957)
  AUTHORS   Ishikawa,G., Kanai,Y., Takata,K., Takeuchi,R., Shimanouchi,K.,
            Ruike,T., Furukawa,T., Kimura,S. and Sakaguchi,K.
  TITLE     DmGEN, a novel RAD2 family endo-exonuclease from Drosophila
            melanogaster
  JOURNAL   Nucleic Acids Res 32 (21), 6251-6259 (2004)
   PUBMED   15576351
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC097377.3 and AC093731.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001130009.2.
            
            Summary: This gene encodes a member of the Rad2/xeroderma
            pigmentosum group G nuclease family, whose members are
            characterized by N-terminal and internal xeroderma pigmentosum
            group G nuclease domains followed by helix-hairpin-helix domains
            and disordered C-terminal domains. The protein encoded by this gene
            is involved in resolution of Holliday junctions, which are
            intermediate four-way structures that covalently link DNA during
            homologous recombination and double-strand break repair. The
            protein resolves Holliday junctions by creating dual incisions
            across the junction to produce nicked duplex products that can be
            ligated. In addition, this protein has been found to localize to
            centrosomes where it has been implicated in regulation of
            centrosome integrity. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because transcript sequence data consistent
            with the reference genome assembly was not available for all
            regions of the RefSeq transcript. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.308.1,
                                           SRR1660807.163351.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381254.7/ ENSP00000370653.2
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-208               AC097377.3         155991-156198
            209-384             AC093731.2         4859-5034
            385-571             AC093731.2         6326-6512
            572-748             AC093731.2         9827-10003
            749-859             AC093731.2         11509-11619
            860-933             AC093731.2         13668-13741
            934-1025            AC093731.2         16126-16217
            1026-1176           AC093731.2         17564-17714
            1177-1213           AC093731.2         18026-18062
            1214-1294           AC093731.2         18149-18229
            1295-1425           AC093731.2         19201-19331
            1426-1487           AC093731.2         22932-22993
            1488-1631           AC093731.2         24908-25051
            1632-9957           AC093731.2         25551-33876
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p24.2"
     gene            1..9957
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="GEN1 Holliday junction 5' flap endonuclease"
                     /db_xref="GeneID:348654"
                     /db_xref="HGNC:HGNC:26881"
                     /db_xref="MIM:612449"
     exon            1..208
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            209..384
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..2950
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="Gen endonuclease homolog 1; Holliday junction
                     resolvase"
                     /codon_start=1
                     /product="flap endonuclease GEN homolog 1"
                     /protein_id="NP_001123481.3"
                     /db_xref="CCDS:CCDS1691.1"
                     /db_xref="GeneID:348654"
                     /db_xref="HGNC:HGNC:26881"
                     /db_xref="MIM:612449"
                     /translation="MGVNDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKM
                     MGSVMKPHLRNLFFRISYLTQMDVKLVFVMEGEPPKLKADVISKRNQSRYGSSGKSWS
                     QKTGRSHFKSVLRECLHMLECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFL
                     YGAQTVYRNFTMNTKDPHVDCYTMSSIKSKLGLDRDALVGLAILLGCDYLPKGVPGVG
                     KEQALKLIQILKGQSLLQRFNRWNETSCNSSPQLLVTKKLAHCSVCSHPGSPKDHERN
                     GCRLCKSDKYCEPHDYEYCCPCEWHRTEHDRQLSEVENNIKKKACCCEGFPFHEVIQE
                     FLLNKDKLVKVIRYQRPDLLLFQRFTLEKMEWPNHYACEKLLVLLTHYDMIERKLGSR
                     NSNQLQPIRIVKTRIRNGVHCFEIEWEKPEHYAMEDKQHGEFALLTIEEESLFEAAYP
                     EIVAVYQKQKLEIKGKKQKRIKPKENNLPEPDEVMSFQSHMTLKPTCEIFHKQNSKLN
                     SGISPDPTLPQESISASLNSLLLPKNTPCLNAQEQFMSSLRPLAIQQIKAVSKSLISE
                     SSQPNTSSHNISVIADLHLSTIDWEGTSFSNSPAIQRNTFSHDLKSEVESELSAIPDG
                     FENIPEQLSCESERYTANIKKVLDEDSDGISPEEHLLSGITDLCLQDLPLKERIFTKL
                     SYPQDNLQPDVNLKTLSILSVKESCIANSGSDCTSHLSKDLPGIPLQNESRDSKILKG
                     DQLLQEDYKVNTSVPYSVSNTVVKTCNVRPPNTALDHSRKVDMQTTRKILMKKSVCLD
                     RHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKLSSPKIHIKETEQCVRS
                     YETAENEESCFPDSTKSSLSSLQCHKKENNSGTCLDSPLPLRQRLKLRFQST"
     exon            385..571
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            572..748
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            749..859
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            860..933
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1025
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1176
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1213
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1294
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1425
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1487
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1631
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..9957
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5729
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="major polyA site"
     regulatory      5802..5807
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="hexamer: AATAAA"
     polyA_site      5820
                     /gene="GEN1"
                     /gene_synonym="Gen"
     polyA_site      6346
                     /gene="GEN1"
                     /gene_synonym="Gen"
     polyA_site      6399
                     /gene="GEN1"
                     /gene_synonym="Gen"
     regulatory      6920..6925
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="hexamer: ATTAAA"
     polyA_site      6938
                     /gene="GEN1"
                     /gene_synonym="Gen"
     regulatory      9780..9785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="hexamer: AATAAA"
     polyA_site      9801
                     /gene="GEN1"
                     /gene_synonym="Gen"
     regulatory      9927..9932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEN1"
                     /gene_synonym="Gen"
                     /note="hexamer: ATTAAA"
     polyA_site      9957
                     /gene="GEN1"
                     /gene_synonym="Gen"
ORIGIN      
        1 gctggattcg aaacgcttcc tggtgctcct gggcctggcg gttgagcccg gggagctagt
       61 ctttgcttgg gtaaagaaag aggactttcc tttttttttt tccttttgag aaaattcaga
      121 aatttggagg cacagtagtt aggattcagg ccaaactggt aaatgacgaa ggggcttctt
      181 ccagagccaa ggaggaaggg ttgtccggca gataatcacc agaatgggag tgaatgactt
      241 gtggcaaatt ttggagcctg ttaagcaaca catccccttg cgtaatcttg gtgggaaaac
      301 cattgcagtt gatctgagtc tctgggtgtg tgaggcacag acagtcaaaa aaatgatggg
      361 cagcgtcatg aagccccacc tcaggaactt attttttcgt atctcatatt taacacaaat
      421 ggatgtaaaa ctggtatttg ttatggaagg ggaaccacca aagctgaaag ctgatgtcat
      481 aagcaagagg aatcagtctc ggtatgggtc ttctggaaaa tcgtggtctc agaaaacagg
      541 gagatcacat tttaaatcag tcttaagaga gtgcctccat atgctcgaat gcttaggaat
      601 cccctgggtt caggctgctg gggaagctga agccatgtgt gcttatctca atgctggtgg
      661 tcatgtcgat ggctgcctca ccaatgatgg agatactttc ctttatgggg cccagactgt
      721 ttacaggaat ttcactatga atacaaagga cccacatgtt gactgttaca caatgtcatc
      781 tatcaagagt aaactaggtt tggatagaga tgctctggtt ggattagcaa tacttcttgg
      841 ctgtgattat ctcccaaagg gagtccctgg agttggaaaa gagcaagcat taaaacttat
      901 acagattttg aaagggcaaa gtttacttca gaggtttaat cggtggaatg aaacatcttg
      961 taactctagt ccacaactgc tagtcactaa aaaactggct cattgttccg tatgttccca
     1021 tccaggttca cctaaggatc atgaacgtaa tggatgcaga ttatgtaaaa gtgataaata
     1081 ttgtgagcca catgactatg aatactgctg tccttgtgag tggcaccgta cagaacatga
     1141 taggcaactc agtgaagtag agaacaatat taagaagaaa gcttgctgtt gtgagggatt
     1201 cccattccat gaggttattc aagaattcct tttaaacaag gataaattgg tgaaggttat
     1261 caggtaccaa agacctgatt tgttattgtt tcagagattt actcttgaaa aaatggagtg
     1321 gcccaatcac tatgcatgtg agaaattgct ggtacttttg acccattatg acatgataga
     1381 aagaaagctt ggtagcagaa actctaatca actacagcca attcgaattg ttaagactcg
     1441 aatcagaaat ggagttcatt gttttgaaat agaatgggaa aagcctgaac attatgctat
     1501 ggaagataaa caacatggag aatttgcttt attaacaatt gaggaagaat cattgtttga
     1561 agcagcatat cctgagatcg ttgctgttta ccaaaaacaa aagttagaaa ttaaagggaa
     1621 gaaacaaaaa cgtattaagc ctaaagaaaa caatttgcca gaaccagatg aagtaatgag
     1681 ctttcagtca cacatgactt taaaacccac atgtgaaatc tttcataagc agaattccaa
     1741 gttaaattcg gggatttccc ctgatcctac attaccacag gaatctattt ctgcctcatt
     1801 gaatagcttg cttttaccta aaaatactcc atgtttgaat gcacaagaac agttcatgtc
     1861 ttctctaaga cctttggcta tacagcaaat taaagctgtc agtaagtctc taatttcaga
     1921 atctagtcaa cccaatacct catctcataa tatatccgtg attgctgatc tacacttgag
     1981 cactattgac tgggaaggta cttcttttag taattctcca gctattcaaa ggaatacttt
     2041 ttctcatgat ttaaaatcag aagttgaatc agagctatca gccatccctg atggctttga
     2101 aaatatccca gaacaactgt cctgtgaatc agaaaggtac actgcaaaca taaagaaagt
     2161 gttggatgag gattctgatg ggattagtcc tgaagagcat ctactttctg gcattactga
     2221 tttatgtctt caggatttgc ctttaaagga acgaatattt acaaaattat catatcctca
     2281 ggataatcta caaccagatg tcaacctgaa aactttgtcc atacttagtg taaaagaatc
     2341 ttgtattgct aacagtggtt ctgattgtac atcacatctt tcaaaggatc ttccaggaat
     2401 tcccttgcaa aatgaatcca gagactctaa aattctaaaa ggagaccagc tgcttcaaga
     2461 agactataaa gtcaatactt ctgtccctta ttctgtcagt aacacagtgg taaagacctg
     2521 caatgttaga ccaccaaata ctgctttaga tcatagtaga aaagttgata tgcaaaccac
     2581 tcggaaaatt ttaatgaaga agagtgtttg ccttgacaga cattcctctg atgaacaaag
     2641 tgccccagtg tttgggaaag ctaagtacac aactcaaaga atgaagcaca gttctcaaaa
     2701 gcataattca tcccatttca aagaaagtgg ccataacaag ttgagtagcc ctaagataca
     2761 tattaaagaa actgaacagt gtgtcagatc ttatgaaaca gctgaaaatg aagaaagctg
     2821 tttcccagat tcaacaaaaa gttctctgag ttctctacaa tgtcataaga aagaaaacaa
     2881 ctctggtact tgtttggata gccctcttcc tttacgccag agattaaaac taagattcca
     2941 aagcacttga aatttaaaac acttaggtat aacttaacta ttttagtact atcagcaata
     3001 gcagagacag agggaaggta tctagttcat gtgtggtaaa aattttaatg ttctctgtgt
     3061 catgaaacac ttgccatttt aatcaaagtt gtaattttta aaaagtcacc taaaactctg
     3121 gttttaaaag atcctctgta ttgaaaactt ctgataatgt atgtcattat gtccttacta
     3181 ttccttaatt gtagttttaa aatattggta tagtacttga cagagtaaat acttcatctg
     3241 attgttcatt tttacttttt cttccacaag cctctaaagt atttatattc cagcttgttc
     3301 ccaagaggat aattctttat acttctcttc attcttttaa ggccttgcaa ggtcttccgt
     3361 tataactcgc tttcctaaaa gctattttct ccctcagtgt gaagatacct ttagtgtgct
     3421 cttccacttt ggaaggccat gcaaggtgtt ccattataac tctttttcct aagagttata
     3481 ttctccctca gtttgaagat acctttagtg tgctcttcca cttttggtgt cttagtctct
     3541 tttgaggggc aaaaatagaa aaggagagaa gatgtcagta tgtttagtaa aaatcatgct
     3601 cgtaatggct gaataaactg agcaaagtaa ctccttatgt atccccagaa gttcacaggt
     3661 atatcgggtg gaaaaagatt tggaaaatca aatgtataac caaaaggatt agaaactagc
     3721 ccaggatcac gtagctaact aataatcctg tgggaatcag ttttcttgcc tgctaatttt
     3781 tttatttttc tattttttcc ttctatagca cttttccccc ttttgttttg aatctatgca
     3841 atattgactt taataccacc aaatattaag tcatgcatta atttaggtcg cactcaaaaa
     3901 ttctagagag gcatccagat tgaaaaggaa aatggtgtct gcagatgaca agattgtatg
     3961 catcaaaaat tctaagaaat ccactagaaa actattaaaa ctgataaagc gagttcatcc
     4021 aggtcgtaga atacaagacc aatgtgcaat gatcattgca ttttgttttt gtttgtttgt
     4081 ttttttttga gacagagtct cactcttgtc gagactgggg tgtagtggcg ccatcttggc
     4141 tcactgcaac ctctgcctcc caggttcacg caattcacat gcctcagcca cctgagtagc
     4201 agggattaca ggtgtgtgcc accatgcctg gctaattttt agtattttta gtagagatgg
     4261 gattttccct tgttggtcag gctagtcatg aactcctggc ctcaagcgat ccacccatct
     4321 tggcctccca gagtgctggg attacaggtg tgaatcacca tactcagcct caattgtatt
     4381 tctgtacacc tctaaataaa tggaaataca ttccatgttc atgaatcaga agacataaga
     4441 tgaccatact ccccaaactg atctacaggt taaatgtaat tcccattaat tccagctggt
     4501 ttctttgctg attctaaaat tcatatggaa atttcaggaa cacagaatat taggaagaat
     4561 cttgaaagag tttgaatgac ccacccttcc tgattttaag tatacagaac tacagtaatg
     4621 aatgtgttgt actggtataa aggtagacat ataggtcaat atagatcaat ggcatagaat
     4681 tgagaattca gaaaaaaagc cttcacgttt atggtcagtt gattttaaac cagagtgcca
     4741 agacaattca atgggaaaag gaaacagtct ttgaaccaac tgtcttctgg atctattgga
     4801 tatctatgta aaaaagagtg acatttgatt cctacttcac atgatattta agaagtaact
     4861 taagatggat caaataccta aatgtaagct aaagctataa aactcttaga agaaaacata
     4921 gacgtaaatc tttgtgactt tggattaggc aaggatttct taaatatacc caaagcatag
     4981 gcaacaaaag agaatacaca aactggactt catcaaaact aaaaactgtt gtttgaaatg
     5041 acaccatcaa gaaagtgaaa agacagctta cagaatggga gaaaatattt gcaaatcata
     5101 aatgtgataa gggacttgta tcttggtggt atataaagaa ctcgtaactc aattataaaa
     5161 agacaacttt gaaatgggca aaatagctca acaggctttt ctgcaaagaa gaaatacaaa
     5221 tgaccaagaa gcacatttaa aaattttcag tactattact catcaggaaa atgcaaatca
     5281 aaaccacaag acacccaatg tctacaatca aaaagataat aactagtatt gatgaggatg
     5341 tggagaaatt gaaattctca taacatgctg gtaggaatgt aaaatggggc agccactttg
     5401 gaaaaagtct ggtagttctt caaatggtta aatgtagagt tacgatatga tccagcaatt
     5461 cctctcccag gtatataccc aagataaatg aaaacttata tccacataaa aacctgtgca
     5521 caaatgtcca tagcagcgtt attcataata gcctaaaagt ggaaacaatc ccagttccag
     5581 aatgaggaag gggagaaact aatgtgtatt agctattgtg tgctaagcat tcaactagat
     5641 tatttacaaa ccttgtatca tctcaactct ttaaggactg tattgcaatg ttttgaatat
     5701 tcagagagaa aaaagtcgtt gctaaaacat tttccaaggt tctgcttatt ctgatttgtt
     5761 cagtcgtggc tgtgatagtt caggaccatc tagaccaggt aaataaaata tctagaggca
     5821 ttcttgagat tgtatgagat gaaaataaca aaattagttg ggagtggcca gtctgagttc
     5881 attttgctat atagctcagg agtccccaac ccctgggcta tggacaggta ccagtccatc
     5941 acctgtcagg aaccggcctg cacagcaggt gttgagcggc atgcaagcga gaattaccgc
     6001 ctgagctctg cctcctgtca gatcagcggt ggcattagag tctcatagga gcatgaaccc
     6061 tattgtgaac tacgcatgcg aggatctagt ttgtgcactc ctactgagaa tctaatgcct
     6121 gatgatataa ggtggaacag cttcatccca cagccatctc cccctcatca tccgtggaaa
     6181 aactgttttc cacgaaagtg gtcctcggtg ccagaaaggt tggggactgc tgatatacct
     6241 aatataggca taagtgtaaa attatagttc ctcctcctca aggatttctc tgttttcatt
     6301 gtccaggtta taccaattct ttttaaagca ttccatgttc ttgttccttg atgtggtcat
     6361 gttatcactt gtaaatcaaa tcttttaagt tgctgcttac gtatttgctc atcacttcat
     6421 tgtcttgttg aggatgcctt gcttcccttt gaaatactct gccaaagatc tgcctccagc
     6481 tgctaggatc ctgtaattgg tttctttctg atggctccat gctaaggccc tgctctggcc
     6541 taactgcttt gggctttgtg taatcaatct taagtccttg gcggtttttc ttttagattt
     6601 ttttccatat aaaatctcta tctggccaca catggtggcc gtgcctgtaa tcccagcacg
     6661 ctgggaggct gaggcgggtg gattgcttga ggccaggagt tcaagaccag cctggccaac
     6721 atggtgaaac cccatctcta ctaaaaatac aaaaatcagt ggggcctggt ggcgtgcacc
     6781 tgtaatccca gctactcggg aagctgaggc aggagaatcg cttgaacctg ggaggcagag
     6841 gttgcagtga gctgagatag tgccactgca cttcactcca ggatgggcga aaaagtgtct
     6901 aaaaaaaatt aaaatttaaa ttaaaaaaaa atatacaatc tccgtcttct agattttata
     6961 atgaggactc tactttttcc ttttgggtag catggcttga gccatcttgt caggcatacc
     7021 tagcttcaga ttcttttctg ctaacagatt ttctctgtga ccctggagac attttctaac
     7081 ttttctgaga ctcatttatt caaatgttta tgtgacaggc attgtgctgg gccctggaga
     7141 tacagtgatc aatggcatcc tataaggttt tgatatctgt gaagtcactg ccaccattga
     7201 cacattctag ggactcagta aattgtagct gtgtccgata ccatcatttg gaaagagcac
     7261 tatcagattt tggattcaaa ctgaatgcag ccaagcactg ccttttggta aaaatgtgga
     7321 cacagtaaca ctagtagggg gcagtgtgag ggtgggagat gtgttttcag aagaaggaaa
     7381 ctctagatta gcatcacaca agggatccta acgtggcatt tgagctactg gaaaaaaatt
     7441 gtgcttgctt aaatataatg tggtaactaa tattcatcta atcacctacc acatttttgg
     7501 agcacccact ttgagcaagg ttcttaggct tttctatgta caacagccag ctggaggccc
     7561 agtgagttta aaatttttcc aggtcaccca actagaaaaa gttaaagctg ggactagaac
     7621 tcagatctcc taattcatga gtgaaaacaa ttctctgtat ttatgtttcc atacatcatt
     7681 cagttcttga ctcaaatatc acctcagaga gtcctaatct ttttatctcc tgacattata
     7741 aatgttgagt tcagggtttt ttttttcatt gtttcccaaa ctagaatgca gacttgatga
     7801 gggcagggac taagtccttt ctgttcacag aattctagaa cagtgtttga cacagtaact
     7861 actagatgca tgaatgacaa ttctccacaa cgccccaccc atggagcctg taccagcagt
     7921 ggctgcactt aaccttgcgg aatctggcct tagtacttca gcatggctca caaggctgag
     7981 gcagcctggc cccaacttgt ctcttcagct tcatgcattc atagtcatga accttggctc
     8041 tagccaccta agaccatttg ttattccctg cacggatgtc tctgcctgaa atgcttgctg
     8101 cttccttatt ggctagataa acctcatcta ttaaggacca gctcacatgt cataacttta
     8161 taatcccaag cagaattatt tgctctatac gtgtccccaa agaaattacc catacattca
     8221 tcagagtatt tatcacattc attcattcgt tccacaaata ttagctgatg cctgccgtgt
     8281 aggaggcaca atattcctta cttaaaaatg cctctatctc tccctcagat aagactaaga
     8341 tctctgagca caggaatcaa gtcttatttc tgttcacttc ccccagatgc ctacacaggg
     8401 tttgactctt ctgcctcacc aactcttacc catcgagatt cagtgtaaac gtggtcttct
     8461 ctaagaaagt cttctgttgc ttctctggat cgctgctagt ccccatagac ttctaaaata
     8521 ttatctgtct atagttttgt gttgggttac attttttgtt tcccacacca gactatgaac
     8581 atcctcaggg cagagaaact atattgtact tctgaattta tagcacccgg cagagggatt
     8641 ggcacagaaa gtgtccagtg aggccgggtg tgggggctca catcagcagt ttgggaggcc
     8701 gagatgggca gattgcctga ggtcaggagt tcgagaccaa cctggccaac atggtgaaat
     8761 cccatctcta caaaaaatac aaaaattagc cgggtgtggt gatgggtacc tgtaatccca
     8821 cctacttggg aggctgagac aagagaatca cttgaacccg ggaggcggag gttgcagtga
     8881 gctgagaccg tgccattgca ctctagcctg ggcaagaaga gcgaaactcc atctaaacaa
     8941 aagaaaaaag aaagtgttca gtgaatgggt gctgattgac tgatggtgat ttcagtcagg
     9001 gtcttagttc taaatttctc tgtggagaga gaccaaatct aaccgggtgg attagcttga
     9061 tacctagtcg cctttagcca cttcaagacg ccctgccaag atgcttgctt tctgaaggtg
     9121 aagagtctaa atcaactgct gttcctgttc tgctggtagc acttagggta aaacagctta
     9181 aatagtatta ctgaaggcag cctcctttca tctatatatt aacgtggcaa tgtttatcac
     9241 cttcttaacc aagcaaagct tttatcaggc tcagctaaca gcttgcatta gcctctttca
     9301 ggaacttgcc acattgccta taaactgaca tgctgcagag tcagccccag caatagtcca
     9361 aaacagtggg actctttttg atgatttcaa aatggatagt ttagttagcc ccaggccaga
     9421 taaattcact aagttgcctg tttggcaatg gagggcagag ttatcagcaa agtaggctga
     9481 cccttctgtg ggcagaatga atattttgct aatgtttaga agaattcctt ttgtaagcat
     9541 caaagaagaa ctttaataag gattaagtgt ctgagcttcc ttagcattat tctattccaa
     9601 gacccaaaaa ggaaaataac ctccccaaat ggtaaaaact gcttgtatat ttagaattct
     9661 ctgatcaaag acctaatgaa aagattctat agtgtttgcg tcatcataag ttaaatcttc
     9721 atctaccacc caactgatac ctgcctttct gtgagtgaaa tcagaaacgg ctgttcttta
     9781 ataaatgctt tcaatctgaa agtcagaaag tgatttatga aacacccagg tgaggggaga
     9841 ctacaagtcc ctttgccttt tagttttcaa gcaatactgg ttgcagtaac tgctctgatg
     9901 taattattgc taacatttac gtttttatta aactgaaaat gctaactgtg aatgcca
//
