LOCUS       NM_015465               5404 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens gem nuclear organelle associated protein 5 (GEMIN5),
            transcript variant 1, mRNA.
ACCESSION   NM_015465
VERSION     NM_015465.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Zhang,J., Liu,X., Zhu,G., Wan,L., Liang,Y., Li,N., Huang,M. and
            Yang,G.
  TITLE     Expanding the clinical phenotype and genetic spectrum of GEMIN5
            disorders: Early-infantile developmental and epileptic
            encephalopathies
  JOURNAL   Brain Behav 14 (5), e3535 (2024)
   PUBMED   38773790
  REMARK    GeneRIF: Expanding the clinical phenotype and genetic spectrum of
            GEMIN5 disorders: Early-infantile developmental and epileptic
            encephalopathies.
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Cascajo-Almenara,M.V., Julia-Palacios,N., Urreizti,R.,
            Sanchez-Cuesta,A., Fernandez-Ayala,D.M., Garcia-Diaz,E., Oliva,C.,
            O Callaghan,M.D.M., Paredes-Fuentes,A.J., Moreno-Lozano,P.J.,
            Muchart,J., Nascimento,A., Ortez,C.I., Natera-de Benito,D.,
            Pineda,M., Rivera,N., Fortuna,T.R., Rajan,D.S., Navas,P.,
            Salviati,L., Palau,F., Yubero,D., Garcia-Cazorla,A., Pandey,U.B.,
            Santos-Ocana,C. and Artuch,R.
  TITLE     Mutations of GEMIN5 are associated with coenzyme Q10 deficiency:
            long-term follow-up after treatment
  JOURNAL   Eur J Hum Genet 32 (4), 426-434 (2024)
   PUBMED   38316953
  REMARK    GeneRIF: Mutations of GEMIN5 are associated with coenzyme Q10
            deficiency: long-term follow-up after treatment.
REFERENCE   3  (bases 1 to 5404)
  AUTHORS   Francisco-Velilla,R., Abellan,S., Garcia-Martin,J.A., Oliveros,J.C.
            and Martinez-Salas,E.
  TITLE     Alternative splicing events driven by altered levels of GEMIN5
            undergo translation
  JOURNAL   RNA Biol 21 (1), 23-34 (2024)
   PUBMED   39194147
  REMARK    GeneRIF: Alternative splicing events driven by altered levels of
            GEMIN5 undergo translation.
REFERENCE   4  (bases 1 to 5404)
  AUTHORS   Fortuna,T.R., Kour,S., Chimata,A.V., Muinos-Buhl,A., Anderson,E.N.,
            Nelson Iv,C.H., Ward,C., Chauhan,O., O'Brien,C., Rajasundaram,D.,
            Rajan,D.S., Wirth,B., Singh,A. and Pandey,U.B.
  TITLE     SMN regulates GEMIN5 expression and acts as a modifier of
            GEMIN5-mediated neurodegeneration
  JOURNAL   Acta Neuropathol 146 (3), 477-498 (2023)
   PUBMED   37369805
  REMARK    GeneRIF: SMN regulates GEMIN5 expression and acts as a modifier of
            GEMIN5-mediated neurodegeneration.
REFERENCE   5  (bases 1 to 5404)
  AUTHORS   Lee,J.H., Horak,C.E., Khanna,C., Meng,Z., Yu,L.R., Veenstra,T.D.
            and Steeg,P.S.
  TITLE     Alterations in Gemin5 expression contribute to alternative mRNA
            splicing patterns and tumor cell motility
  JOURNAL   Cancer Res 68 (3), 639-644 (2008)
   PUBMED   18245461
  REMARK    GeneRIF: The data provide the first demonstration that alterations
            in the expression of Gemin5, a spliceosome protein, can effect both
            specific splicing events and tumor cell motility.
REFERENCE   6  (bases 1 to 5404)
  AUTHORS   Fierro-Monti,I., Mohammed,S., Matthiesen,R., Santoro,R.,
            Burns,J.S., Williams,D.J., Proud,C.G., Kassem,M., Jensen,O.N. and
            Roepstorff,P.
  TITLE     Quantitative proteomics identifies Gemin5, a scaffolding protein
            involved in ribonucleoprotein assembly, as a novel partner for
            eukaryotic initiation factor 4E
  JOURNAL   J Proteome Res 5 (6), 1367-1378 (2006)
   PUBMED   16739988
REFERENCE   7  (bases 1 to 5404)
  AUTHORS   Yong,J., Wan,L. and Dreyfuss,G.
  TITLE     Why do cells need an assembly machine for RNA-protein complexes?
  JOURNAL   Trends Cell Biol 14 (5), 226-232 (2004)
   PUBMED   15130578
  REMARK    Review article
REFERENCE   8  (bases 1 to 5404)
  AUTHORS   Paushkin,S., Gubitz,A.K., Massenet,S. and Dreyfuss,G.
  TITLE     The SMN complex, an assemblyosome of ribonucleoproteins
  JOURNAL   Curr Opin Cell Biol 14 (3), 305-312 (2002)
   PUBMED   12067652
  REMARK    Review article
REFERENCE   9  (bases 1 to 5404)
  AUTHORS   Gubitz,A.K., Mourelatos,Z., Abel,L., Rappsilber,J., Mann,M. and
            Dreyfuss,G.
  TITLE     Gemin5, a novel WD repeat protein component of the SMN complex that
            binds Sm proteins
  JOURNAL   J Biol Chem 277 (7), 5631-5636 (2002)
   PUBMED   11714716
  REMARK    GeneRIF: we report the identification of an additional component of
            the SMN complex, a novel WD repeat protein termed Gemin5. Gemin5
            binds SMN directly and is a component of the SMN complex
REFERENCE   10 (bases 1 to 5404)
  AUTHORS   Sleeman,J.E. and Lamond,A.I.
  TITLE     Newly assembled snRNPs associate with coiled bodies before
            speckles, suggesting a nuclear snRNP maturation pathway
  JOURNAL   Curr Biol 9 (19), 1065-1074 (1999)
   PUBMED   10531003
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA400244.1, BC036894.1,
            BC014147.2, BX329222.2, AK074066.2, AL117665.1 and AC008421.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015465.4.
            
            Summary: This gene encodes a WD repeat protein that is a component
            of the survival of motor neurons (SMN) complex. The SMN complex
            plays a critical role in mRNA splicing through the assembly of
            spliceosomal small nuclear ribonucleoproteins (snRNPs), and may
            also mediate the assembly and transport of other classes of
            ribonucleoproteins. The encoded protein is the snRNA-binding
            component of the SMN complex. Dysregulation of this gene may play a
            role in alternative mRNA splicing and tumor cell motility.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Nov
            2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK074066.2, BC113614.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000285873.8/ ENSP00000285873.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                DA400244.1         6-30
            26-2306             BC036894.1         11-2291
            2307-3836           BC014147.2         470-1999
            3837-4425           BX329222.2         50-638              c
            4426-5292           AK074066.2         4403-5269
            5293-5399           AL117665.1         4360-4466
            5400-5404           AC008421.6         97220-97224
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.2"
     gene            1..5404
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /note="gem nuclear organelle associated protein 5"
                     /db_xref="GeneID:25929"
                     /db_xref="HGNC:HGNC:20043"
                     /db_xref="MIM:607005"
     exon            1..244
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..4605
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="gem-associated protein 5 isoform 1"
                     /protein_id="NP_056280.2"
                     /db_xref="CCDS:CCDS4330.1"
                     /db_xref="GeneID:25929"
                     /db_xref="HGNC:HGNC:20043"
                     /db_xref="MIM:607005"
                     /translation="MGQEPRTLPPSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPG
                     AGESPGTPPFRVIGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKTVV
                     TEHALHQHTISTLHWSPRVKDLIVSGDEKGVVFCYWFNRNDSQHLFIEPRTIFCLTCS
                     PHHEDLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLPGEDCLSINQEE
                     TSEEAEITNGNAVAQAPVTKGCYLATGSKDQTIRIWSCSRGRGVMILKLPFLKRRGGG
                     IDPTVKERLWLTLHWPSNQPTQLVSSCFGGELLQWDLTQSWRRKYTLFSASSEGQNHS
                     RIVFNLCPLQTEDDKQLLLSTSMDRDVKCWDIATLECSWTLPSLGGFAYSLAFSSVDI
                     GSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPTKEGCLAFGTDDGK
                     VGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGEGDRPSLALYSCGGEGIVL
                     QHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIEIFQIP
                     NLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLKTVIESSPES
                     PVTITEPYRTLSGHTAKITSVAWSPHHDGRLVSASYDGTAQVWDALREEPLCNFRGHR
                     GRLLCVAWSPLDPDCIYSGADDFCVHKWLTSMQDHSRPPQGKKSIELEKKRLSQPKAK
                     PKKKKKPTLRTPVKLESIDGNEEESMKENSGPVENGVSDQEGEEQAREPELPCGLAPA
                     VSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLIKKRKARSLLPLSTSLD
                     HRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGKGHL
                     ENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQL
                     CFQDQYVKAASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPVLKDLYLSWG
                     TVLERDGHYAVAAKCYLGATCAYDAAKVLAKKGDAASLRTAAELAAIVGEDELSASLA
                     LRCAQELLLANNWVGAQEALQLHESLQGQRLVFCLLELLSRHLEEKQLSEGKSSSSYH
                     TWNTGTEGPFVERVTAVWKSIFSLDTPEQYQEAFQKLQNIKYPSATNNTPAKQLLLHI
                     CHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDKL
                     GDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDT
                     ASTEETDPETSQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQ
                     ETLAEMIRQHQKSQLCKSTANGPDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTK
                     RLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIRSHFPGCLAQEMQQQAQELLQKYG
                     NTKTYRRHCQTFCM"
     exon            245..405
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            406..587
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            588..739
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            740..859
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            860..992
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1158
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1371
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1457
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1540
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1677
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1751
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1933
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2073
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2245
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2473
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..2587
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2710
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2806
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2944
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3092
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            3093..3212
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            3213..3423
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3675
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            3676..3838
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            3839..4340
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            4341..4437
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..5404
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5225..5230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /note="hexamer: ACTAAA"
     polyA_site      5246
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /note="major polyA site"
     regulatory      5311..5316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /note="hexamer: AATAAA"
     polyA_site      5339
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
     regulatory      5374..5379
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
                     /note="hexamer: ATTAAA"
     polyA_site      5404
                     /gene="GEMIN5"
                     /gene_synonym="GEMIN-5; NEDCAM"
ORIGIN      
        1 gccccgctcc ctacctaagg cgtgaggcta cgagcggtcg gctgtggcag cttctcttgt
       61 ctctgacggc ttgtagttat ggggcaggag ccgcggacgc tgccgccctc ccccaactgg
      121 tactgcgccc gctgcagcga tgccgtgccc gggggcctct ttggcttcgc cgcgcggacc
      181 tccgtcttcc ttgtccgcgt gggcccgggc gcaggcgaga gtccagggac acccccgttt
      241 cgagtcatag gagagttggt gggacacacc gaaagggtct ctggcttcac attttctcat
      301 caccctggtc agtacaacct ctgtgccacc agctccgacg atgggactgt gaaaatatgg
      361 gatgtagaga caaaaacagt tgtgacagaa catgcactcc atcagcatac gatatcaaca
      421 ttacattggt ctcctcgagt aaaggactta atagtatctg gggatgaaaa aggagtagtt
      481 ttctgttact ggtttaacag aaatgacagc cagcacctct ttatagaacc caggacaatt
      541 ttctgtctta cttgttcacc tcatcatgaa gatttagtag ccattggcta caaggatggc
      601 atagtggtga taattgacat cagtaagaaa ggagaagtta ttcataggct tcgaggccat
      661 gatgatgaaa tccactccat agcctggtgt cccctgcctg gtgaagattg tttatctata
      721 aaccaagagg aaacttcaga agaagctgaa attaccaacg ggaatgctgt agcacaagct
      781 ccagtaacaa aaggttgcta cttagccact ggaagcaaag atcaaaccat tcgaatctgg
      841 agctgttcta gaggccgagg ggtgatgatt ttgaaattgc cctttctgaa gagaagagga
      901 gggggtatag acccaactgt taaagagcgc ctttggttga cactccattg gcccagcaat
      961 caaccaacac agctggtatc tagctgtttt ggaggtgaac tgttgcaatg ggatctcact
     1021 caatcttgga gacggaaata caccctcttc agtgcctcat cagaagggca aaatcattca
     1081 agaattgtgt ttaatttatg tcctttacaa acagaggatg acaaacagct attactttct
     1141 acatcaatgg atagagatgt aaaatgttgg gacatagcca ccttggagtg cagctggacc
     1201 cttccttccc ttggtgggtt tgcatacagc ctggctttct cttctgtgga cataggctct
     1261 ttggccatag gtgttgggga tggcatgatc cgtgtatgga atacactctc cataaagaac
     1321 aactatgatg tgaaaaattt ttggcaaggc gtgaagtcca aggttacagc gctgtgctgg
     1381 cacccaacca aggaaggttg cttagctttt ggaactgatg atggaaaagt gggattgtat
     1441 gacacctact ccaacaagcc tccacagatt tctagcacat atcataagaa gactgtatat
     1501 actttagcct gggggccacc agtacccccc atgtcacttg gaggagaagg agacagacct
     1561 tcccttgctt tatacagctg tggaggagaa gggattgtct tacagcataa tccctggaag
     1621 cttagtggag aagcctttga catcaacaaa ctcatcaggg acaccaattc aatcaaatac
     1681 aaattgcctg tacacacaga gataagttgg aaagcagatg gcaaaatcat ggctcttggc
     1741 aatgaagatg gatcaataga aatatttcag attcccaacc tgaaactgat ctgtactatc
     1801 caacagcatc acaagcttgt gaataccatt agctggcatc atgagcatgg cagccagcca
     1861 gaattgagct atctgatggc ctctggctcc aacaatgcag tcatttacgt gcacaacctg
     1921 aagactgtca tagagagcag ccctgagtct ccagtgacca ttacagagcc ctaccggacc
     1981 ctctcagggc atacggccaa gattaccagt gtggcgtgga gcccacatca tgatggaagg
     2041 ctggtatctg cttcctatga tggtacagcc caggtgtggg atgctctccg ggaagagccc
     2101 ctgtgcaatt tccgaggaca tcgaggtcga ctgctttgtg tggcatggtc tcctttggat
     2161 ccagactgca tctattcagg ggcagatgac ttttgtgtgc acaagtggct cacttccatg
     2221 caagatcatt cccggcctcc tcaaggcaaa aaaagtattg aattggagaa aaaacggctc
     2281 tctcaaccta aggcaaagcc caaaaagaag aaaaagccca ccttgagaac tcctgtaaag
     2341 ctggaatcga ttgatggaaa tgaagaagaa agcatgaagg agaactcagg acctgttgag
     2401 aatggtgtgt cagaccaaga aggggaggag caagcacggg agccggaatt accctgtggc
     2461 cttgctccag cggtttctag agaaccagtt atctgcactc cagtttcctc aggctttgaa
     2521 aagtcaaaag tcaccattaa taacaaagtc attttactga aaaaggagcc accaaaagag
     2581 aagccagaaa ccttaatcaa gaagagaaaa gctcgttcct tgcttcccct gagtacaagc
     2641 ctggaccaca gatccaaaga ggagcttcat caggactgtt tggtactagc aactgcaaag
     2701 cactccagag agctgaatga agatgtgtct gctgatgttg aggaaagatt tcatctgggg
     2761 cttttcacag acagggctac cctgtataga atgattgata ttgaaggaaa aggtcactta
     2821 gaaaatggcc accctgagtt atttcaccag cttatgcttt ggaaaggaga tctcaaaggt
     2881 gttctccaga ctgcagcaga aagaggggag ctgacagaca accttgtggc tatggcacca
     2941 gcagctggct accatgtgtg gctatgggct gtggaagctt ttgccaaaca gctgtgtttt
     3001 caggatcagt atgtcaaggc tgcttctcac ctactttcca tccacaaagt gtatgaagcg
     3061 gtggagctgc tcaagtcaaa ccatttttac agggaagcta ttgcgattgc caaggcccgg
     3121 ctgcgcccgg aggacccagt cctgaaggac ttgtacctca gctggggaac cgtcctagaa
     3181 agagatggcc actatgctgt agctgccaaa tgctatttag gggccacttg tgcttatgat
     3241 gcagccaaag ttttggccaa aaagggggat gcggcatcac ttagaacggc tgcagagttg
     3301 gctgccatcg taggagagga tgagttgtct gcttccctgg ctctcagatg tgcccaagag
     3361 ctgcttctgg ccaacaactg ggtgggagcc caggaagccc tgcagctgca tgaaagtcta
     3421 cagggtcaga gattggtgtt ttgccttctg gagctactgt ccaggcatct ggaggaaaag
     3481 cagctttcag agggcaaaag ctcctcctct taccacactt ggaacacggg caccgaaggg
     3541 cctttcgtgg agagggtgac tgcagtgtgg aagagcatct tcagccttga cacccctgag
     3601 cagtatcagg aagcctttca gaagctgcag aacatcaagt acccatctgc tacaaataac
     3661 acacctgcca aacagctcct gcttcacatt tgccatgact tgaccctggc agtgctgagc
     3721 caacagatgg cctcctggga cgaggctgtg caggcgctcc ttcgggcggt ggtccggagc
     3781 tatgactcag ggagcttcac catcatgcag gaagtgtact cagcctttct ccctgacggc
     3841 tgtgaccacc taagagacaa gttgggggac catcaatccc ctgccacacc agctttcaaa
     3901 agtttggagg ccttttttct ttatgggcgt ctgtatgaat tctggtggtc tctctccaga
     3961 ccttgcccaa attccagtgt ctgggtaagg gctggtcaca gaacactctc tgttgagcca
     4021 agccagcagt tagacactgc cagcactgaa gaaacggacc ctgaaacttc tcagccagag
     4081 ccaaacaggc cttcagaact agacttgaga ctcacagaag aaggtgagcg aatgctgagt
     4141 acttttaagg agctcttttc agaaaagcat gccagtctcc aaaactcaca gagaactgtt
     4201 gctgaagtcc aagagacctt ggcagaaatg atccgacaac accaaaagag tcaactctgt
     4261 aaatccacag caaatggtcc tgataagaat gaaccggaag tagaagcaga gcagcccctc
     4321 tgcagttctc agagccagtg taaagaagaa aaaaatgagc cactttctct gcctgagtta
     4381 accaaaaggc ttaccgaggc aaatcagaga atggcgaaat ttcctgagag cattaaggcc
     4441 tggcccttcc cagatgtgct ggagtgctgc ctcgtcctgc ttctcatcag gtcccacttt
     4501 cctggctgtc tggcccagga aatgcagcag caggcccaag agctccttca gaaatacggc
     4561 aacacgaaaa cttacagaag acactgccag accttctgta tgtgaatttt cacacacctt
     4621 gaagaaactg ccaaattgaa aatgtttgac atctttcacc tctgcagtta tgcctcacca
     4681 gacattcact ctggtcccta gatgtttttg cagtaatcca aaagaataca aacaaggatt
     4741 aagtttgaat caaccctgcc tacccataga caacggtgga tctgacttta gactcaattg
     4801 tggtctccta ctggagggaa gatcatgaaa agcccacagt agttattcag aactaacacc
     4861 tgcagagtgt tggtcatctc tacagcctta ggcaggtttc acccaaagag gagaaacttc
     4921 tgtcgtcacc caaagtgtta catgcttaaa acacaagcta cctttgtaaa tacttcatct
     4981 gatcagaagt gtgtcatgct tgtttgagat ggagttgctg cattttagga ctattgatac
     5041 ctttttttaa ttgtttttat aatatttaat ttgaaagagg agacccttct ctctctactc
     5101 tttcatagac tgaagtttga atatgaaata ggccttaacc atcatgttga ctctcctgtc
     5161 agaattttag gttggaaatt tggttttatt ctttcatgta attgcttatt tgaacagatc
     5221 acttactaaa gctttagaag aagtgattca aatgtgtgtt ttcccttcag ttttataaca
     5281 aatggattga tggcagtcaa atagctcagg aataaattac tgtttcaatg gttcttaaac
     5341 tttcttggat cataggatcc ttttgagaat cagattaaag ccaaagatac tctttggaga
     5401 aaaa
//
