LOCUS       NM_004751               4934 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens glucosaminyl (N-acetyl) transferase 3, mucin type
            (GCNT3), transcript variant 1, mRNA.
ACCESSION   NM_004751
VERSION     NM_004751.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Kang,Q., Tingting,W., Bingzi,D., Hao,Z., Yuwei,X., Chuandong,S. and
            Chengzhan,Z.
  TITLE     GCNT3 regulated MUC13 to promote the development of hepatocellular
            carcinoma through the GSK3beta/beta-catenin pathway
  JOURNAL   Dig Liver Dis 56 (9), 1572-1581 (2024)
   PUBMED   38369410
  REMARK    GeneRIF: GCNT3 regulated MUC13 to promote the development of
            hepatocellular carcinoma through the GSK3beta/beta-catenin pathway.
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Yamamoto,D., Sasaki,K., Kosaka,T., Oya,M. and Sato,T.
  TITLE     Functional analysis of GCNT3 for cell migration and EMT of
            castration-resistant prostate cancer cells
  JOURNAL   Glycobiology 32 (10), 897-908 (2022)
   PUBMED   35867813
  REMARK    GeneRIF: Functional analysis of GCNT3 for cell migration and EMT of
            castration-resistant prostate cancer cells.
REFERENCE   3  (bases 1 to 4934)
  AUTHORS   Zhang,Y., Xiao,P. and Hu,X.
  TITLE     LINC00511 enhances LUAD malignancy by upregulating GCNT3 via
            miR-195-5p
  JOURNAL   BMC Cancer 22 (1), 389 (2022)
   PUBMED   35399076
  REMARK    GeneRIF: LINC00511 enhances LUAD malignancy by upregulating GCNT3
            via miR-195-5p.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4934)
  AUTHORS   Gupta,R., Leon,F., Thompson,C.M., Nimmakayala,R., Karmakar,S.,
            Nallasamy,P., Chugh,S., Prajapati,D.R., Rachagani,S., Kumar,S. and
            Ponnusamy,M.P.
  TITLE     Global analysis of human glycosyltransferases reveals novel targets
            for pancreatic cancer pathogenesis
  JOURNAL   Br J Cancer 122 (11), 1661-1672 (2020)
   PUBMED   32203219
  REMARK    GeneRIF: Global analysis of human glycosyltransferases reveals
            novel targets for pancreatic cancer pathogenesis.
            Erratum:[Br J Cancer. 2020 May;122(11):1726. doi:
            10.1038/s41416-020-0842-6.. PMID: 32286491]
REFERENCE   5  (bases 1 to 4934)
  AUTHORS   Li,Q., Ran,P., Zhang,X., Guo,X., Yuan,Y., Dong,T., Zhu,B., Zheng,S.
            and Xiao,C.
  TITLE     Downregulation of N-Acetylglucosaminyltransferase GCNT3 by
            miR-302b-3p Decreases Non-Small Cell Lung Cancer (NSCLC) Cell
            Proliferation, Migration and Invasion
  JOURNAL   Cell Physiol Biochem 50 (3), 987-1004 (2018)
   PUBMED   30355927
  REMARK    GeneRIF: GCNT3 was regarded as an oncogene in non-small cell lung
            cancer, and a target of miR-302b-3p, which regulated cell
            proliferation, migration and invasion in a GCNT3-dependent manner
            and E-cadherin, N-cadherin, vimentin, cyclin D1 and p-Erk appeared
            to be downstream molecules of the miR-302b-3p/GCNT3 pathway.
REFERENCE   6  (bases 1 to 4934)
  AUTHORS   Tan,S. and Cheng,P.W.
  TITLE     Mucin biosynthesis: identification of the cis-regulatory elements
            of human C2GnT-M gene
  JOURNAL   Am J Respir Cell Mol Biol 36 (6), 737-745 (2007)
   PUBMED   17303715
  REMARK    GeneRIF: Results describe the identification of the cis-regulatory
            elements of human C2GnT-M gene.
REFERENCE   7  (bases 1 to 4934)
  AUTHORS   Huang,M.C., Chen,H.Y., Huang,H.C., Huang,J., Liang,J.T., Shen,T.L.,
            Lin,N.Y., Ho,C.C., Cho,I.M. and Hsu,S.M.
  TITLE     C2GnT-M is downregulated in colorectal cancer and its re-expression
            causes growth inhibition of colon cancer cells
  JOURNAL   Oncogene 25 (23), 3267-3276 (2006)
   PUBMED   16418723
  REMARK    GeneRIF: Frequently downregulated in colorectal cancer and
            suppresses colon cancer cell growth.
REFERENCE   8  (bases 1 to 4934)
  AUTHORS   Beum,P.V., Bastola,D.R. and Cheng,P.W.
  TITLE     Mucin biosynthesis: epidermal growth factor downregulates core 2
            enzymes in a human airway adenocarcinoma cell line
  JOURNAL   Am J Respir Cell Mol Biol 29 (1), 48-56 (2003)
   PUBMED   12600830
  REMARK    GeneRIF: EGF suppressed C2GnT activity in a time- and
            dose-dependent fashion, and also suppressed core 4 beta1,6
            N-acetylglucosaminyltransferase (C4GnT) activity
REFERENCE   9  (bases 1 to 4934)
  AUTHORS   Schwientek,T., Nomoto,M., Levery,S.B., Merkx,G., van Kessel,A.G.,
            Bennett,E.P., Hollingsworth,M.A. and Clausen,H.
  TITLE     Control of O-glycan branch formation. Molecular cloning of human
            cDNA encoding a novel beta1,6-N-acetylglucosaminyltransferase
            forming core 2 and core 4
  JOURNAL   J Biol Chem 274 (8), 4504-4512 (1999)
   PUBMED   9988682
REFERENCE   10 (bases 1 to 4934)
  AUTHORS   Yeh,J.C., Ong,E. and Fukuda,M.
  TITLE     Molecular cloning and expression of a novel beta-1,
            6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I
            branches
  JOURNAL   J Biol Chem 274 (5), 3215-3221 (1999)
   PUBMED   9915862
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP243086.1, BC017032.1 and
            AC092755.4.
            
            On Nov 23, 2018 this sequence version replaced NM_004751.2.
            
            Summary: This gene encodes a member of the
            N-acetylglucosaminyltransferase family. The encoded protein is a
            beta-6-N-acetylglucosamine-transferase that catalyzes the formation
            of core 2 and core 4 O-glycans on mucin-type
            glycoproteins.[provided by RefSeq, Apr 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP243086.1, BC017032.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396065.3/ ENSP00000379377.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               BP243086.1         1-141
            142-1903            BC017032.1         1-1762
            1904-1904           AC092755.4         41727-41727
            1905-2222           BC017032.1         1764-2081
            2223-4934           AC092755.4         42046-44757
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..4934
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="glucosaminyl (N-acetyl) transferase 3, mucin type"
                     /db_xref="GeneID:9245"
                     /db_xref="HGNC:HGNC:4205"
                     /db_xref="MIM:606836"
     exon            1..199
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /inference="alignment:Splign:2.1.0"
     exon            200..389
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /inference="alignment:Splign:2.1.0"
     exon            390..4934
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /inference="alignment:Splign:2.1.0"
     CDS             450..1766
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /EC_number="2.4.1.102"
                     /EC_number="2.4.1.150"
                     /EC_number="2.4.1.148"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4
                     beta-1,6-N-acetylglucosaminyltransferase; C2GnT-mucin
                     type; beta-1,3-galactosyl-O-glycosyl-glycoprotein
                     beta-1,6-N-acetylglucosaminyltransferase 3;
                     beta1,6GlcNAc-transferase; mucus-type core 2
                     beta-1,6-N-acetylglucosaminyltransferase; hC2GnT-M"
                     /codon_start=1
                     /product="beta-1,3-galactosyl-O-glycosyl-glycoprotein
                     beta-1,6-N-acetylglucosaminyltransferase 3 isoform 1"
                     /protein_id="NP_004742.1"
                     /db_xref="CCDS:CCDS10172.1"
                     /db_xref="GeneID:9245"
                     /db_xref="HGNC:HGNC:4205"
                     /db_xref="MIM:606836"
                     /translation="MVQWKRLCQLHYLWALGCYMLLATVALKLSFRLKCDSDHLGLES
                     RESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTH
                     YLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFERLLRAVYAPQNIY
                     CVHVDEKSPETFKEAVKAIISCFPNVFIASKLVRVVYASWSRVQADLNCMEDLLQSSV
                     PWKYFLNTCGTDFPIKSNAEMVQALKMLNGRNSMESEVPPKHKETRWKYHFEVVRDTL
                     HLTNKKKDPPPYNLTMFTGNAYIVASRDFVQHVLKNPKSQQLIEWVKDTYSPDEHLWA
                     TLQRARWMPGSVPNHPKYDISDMTSIARLVKWQGHEGDIDKGAPYAPCSGIHQRAICV
                     YGAGDLNWMLQNHHLLANKFDPKVDDNALQCLEEYLRYKAIYGTEL"
     regulatory      2198..2203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="hexamer: AATAAA"
     polyA_site      2222
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="major polyA site"
     regulatory      3011..3016
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="hexamer: AATAAA"
     polyA_site      3035
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="major polyA site"
     regulatory      4913..4918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="hexamer: AATAAA"
     polyA_site      4934
                     /gene="GCNT3"
                     /gene_synonym="C2/4GnT; C24GNT; C2GNT2; C2GNTM; GNTM"
                     /note="major polyA site"
ORIGIN      
        1 gagtcagagc ctcttctctc taagtcacgg gaactgccct tgctacttgt gacctgccct
       61 ttactcagca gtttttgttc tgggaagccc tgggattctg ctaataccta tcactgtagg
      121 tgctgaaggg aaacagatga agaacatgac ctcaaggagc ttcctgtcaa tgagaagacc
      181 aagctgacgc ctggcaaaga tattaaagag gagcctgaaa ctgttccttg gacatcttat
      241 gaatgtcaga aaataccttt tggagggtta gaagatcagg ggacatggtt gttcacattt
      301 gctgccacgg aacaccgcca gtcttcactt ggaaacagaa tcacgccttg tgaagagatc
      361 atccctaagc aggagagaag ctactaaagg attgtgtcct cctccacctt ccctgtgctc
      421 ggtctccacc tgtctcccat tctgtgacga tggttcaatg gaagagactc tgccagctgc
      481 attacttgtg ggctctgggc tgctatatgc tgctggccac tgtggctctg aaactttctt
      541 tcaggttgaa gtgtgactct gaccacttgg gtctggagtc cagggaatct caaagccagt
      601 actgtaggaa tatcttgtat aatttcctga aacttccagc aaagaggtct atcaactgtt
      661 caggggtcac ccgaggggac caagaggcag tgcttcaggc tattctgaat aacctggagg
      721 tcaagaagaa gcgagagcct ttcacagaca cccactacct ctccctcacc agagactgtg
      781 agcacttcaa ggctgaaagg aagttcatac agttcccact gagcaaagaa gaggtggagt
      841 tccctattgc atactctatg gtgattcatg agaagattga aaactttgaa aggctactgc
      901 gagctgtgta tgcccctcag aacatatact gtgtccatgt ggatgagaag tccccagaaa
      961 ctttcaaaga ggcggtcaaa gcaattattt cttgcttccc aaatgtcttc atagccagta
     1021 agctggttcg ggtggtttat gcctcctggt ccagggtgca agctgacctc aactgcatgg
     1081 aagacttgct ccagagctca gtgccgtgga aatacttcct gaatacatgt gggacggact
     1141 ttcctataaa gagcaatgca gagatggtcc aggctctcaa gatgttgaat gggaggaata
     1201 gcatggagtc agaggtacct cctaagcaca aagaaacccg ctggaaatat cactttgagg
     1261 tagtgagaga cacattacac ctaaccaaca agaagaagga tcctccccct tataatttaa
     1321 ctatgtttac agggaatgcg tacattgtgg cttcccgaga tttcgtccaa catgttttga
     1381 agaaccctaa atcccaacaa ctgattgaat gggtaaaaga cacttatagc ccagatgaac
     1441 acctctgggc cacccttcag cgtgcacggt ggatgcctgg ctctgttccc aaccacccca
     1501 agtacgacat ctcagacatg acttctattg ccaggctggt caagtggcag ggtcatgagg
     1561 gagacatcga taagggtgct ccttatgctc cctgctctgg aatccaccag cgggctatct
     1621 gcgtttatgg ggctggggac ttgaattgga tgcttcaaaa ccatcacctg ttggccaaca
     1681 agtttgaccc aaaggtagat gataatgctc ttcagtgctt agaagaatac ctacgttata
     1741 aggccatcta tgggactgaa ctttgagaca cactatgaga gcgttgctac ctgtggggca
     1801 agagcatgta caaacatgct cagaacttgc tgggacagtg tgggtgggag accagggctt
     1861 tgcaattcgt ggcatccttt aggataagag ggctgctatt agagtgtggg taagtagatc
     1921 ttttgccttg caaattgctg cctgggtgaa tgctgcttgt tctctcaccc ctaaccctag
     1981 tagttcctcc actaactttc tcactaagtg agaatgagaa ctgctgtgat agggagagtg
     2041 aaggagggat atgtggtaga gcacttgatt tcagttgaat gcctgctggt agcttttcca
     2101 ttctgtggag ctgccgttcc taataattcc aggtttggta gcgtggagga gaactttgat
     2161 ggaaagagaa ccttcccttc tgtactgtta acttaaaaat aaatagctcc tgattcaaag
     2221 tattacctct actttttgcc tagtatgcca gaaataatat aaatataaac agataaagtg
     2281 tgtgagactt tttctcataa ctattcatga catttaaaat ccctaggggc tggcaagaga
     2341 gttctcatta ttctgaaatg gtcctgacaa gctgcatgaa tagcaatttt ttttttgaga
     2401 cagagtcttg ctctgtcacc caggctggac tgcagtagtg caatctcagt tcactgcaac
     2461 ctccgcctcc caggttcaag cgatactccc acctcagcct cctgagtagc tgggactaca
     2521 ggcatgcagc accatgtctg gctaattttt gtatttttag tagaggccgg gtttcaccat
     2581 attcgccagg ctggtcttga actcctgacc ttgtgatctg cccgcctcgg ccttccgaaa
     2641 tgctgggatt acaggtggga actactgcgc ctggcctaca aatagcaaat tctaacgaag
     2701 acaggggaac agggatggtt cttccattgt taaaagccat cctcattttg tttatattgc
     2761 caggtttgtg atttttctgt aaaggaaaag gcagggtgat ttaaccagtt tgaccacctt
     2821 tcctgtactc ttacaggaaa atcgcagcac taattctaat tttgtccact ttacagccaa
     2881 agcttagcta atgttccata aaggagataa tagccaatca ggtaaggtaa tgtgtaattc
     2941 attattcaaa gtggaacatg tttttgtagg gggagagtct gcactattaa taattgtatt
     3001 gagaaataaa aataaactag gactattcag ttaaaccagg atgtcttatt attccatgtt
     3061 taggcctctt gagtcaaaac tctttttttt tttttttttt tttttttttt tggagacagg
     3121 ttctcactct gttgcccagg ctggagtgca gtggcatgat cttggctcac tgaagcctct
     3181 gcctcccagg ttcaagtgat tctcccccca aaccttgcaa gtagctggga ttacagatgt
     3241 gagccaccat gcccagctga tttttgtgta tttttagtaa agatggggtt tcaccatgtt
     3301 ggccaggttg gtctcaaact cctggcctca agtgatccac ctgccccagc ctcccaaagt
     3361 gctgggatta caggtgtgag ccaccatgcc tggtcccctc ttgagtcaaa actcttattt
     3421 cagaatgcga atggaaggat cgctattagt gattctgcag attacacatt tccttgcggg
     3481 gagacaataa ggtatgtgta aatacatata tgtgtgtgta tgtatacaca catacagcac
     3541 gctacctccc aagtgttacc tagtgaaaca gtttcctttc cgaagctcca gagatggttg
     3601 ttggctaagt tatactcttc tgtgatgggc aagggatact ataaaaacat aggcagtgcc
     3661 ctttgaatat agtacagctc atcttctgca tacgatatgc cctggaaagg tgatttatat
     3721 gcaagttaat tggtgtaatc tgaaggatgc tcccattaat acgtcatgat gtcattaata
     3781 tgtttcttcc tttctgattt ttgttttttt tttttttttt gagacagagt ctcactctgt
     3841 cacccaggtt ggagtgcaac gaatggcgca atcttggctc attacaacct ccgcctcctg
     3901 ggtccaagcg attctcctgc ctcagcctcc tgagtaactg ggattacagg tgtgtgccac
     3961 cttgcctggc taattttttt gtattttttt agtagaaaca gggtttcacc atgttggtca
     4021 ggctggtctg aactcctgac ctcaagtgat ccacttgcct tggcttccca aagtgctggg
     4081 attacaggcg tgagctaccg cacccagccc tttctgagtg ttgtcattca attcatcaag
     4141 ttttctgtca tgatcaactt tctctatgca aaccctgtaa ctctgacagt tatcactgtg
     4201 tctgacacag tgaatttttt atttataagg tagttacttt tggccaggtg tggtggctga
     4261 cgcctgtaat cccagcactt tgggaggcca aggtaggcag atcacttgag gtcaggagtt
     4321 caagatcagc ctggccaaca tggtgaatcc ccatctctac taaaaatata aaaattagcc
     4381 agacatggtg gtgggtacct gtaatcccag ctacttggga ggctgaggca ggagaatcgc
     4441 ttgaacatgg gaggcagagg ttgcagtgag ctgagatcgt accactgcac tccagcctgg
     4501 gcgatagagc cagactgagt ctcaaaaaaa aaaaaaaagt tacctttttt tggtaaggtt
     4561 gtacttctta gataatggtc attgtcacta ccaacttgcc tgcattgtaa tagagtccta
     4621 ttcactttgc tcccaacccc actacggaga tgactgatga ctagttgtat acacagtggg
     4681 tgtgccctga gaggtctgtt gccagcagtg gtgatcgatc acggcctccc cctgctggct
     4741 gatgtgatgg tccttggtcc tcctctgaag gaggtagaag gggcacacgg ccaccgtggg
     4801 aagtaggcag aaaagcctcc ctgggtgcag attctctgag aaaagaactt tggcctcata
     4861 gttaacttac tccaaaagct ttcatgtgaa ataatttata attttttata taaataaaaa
     4921 gattaaaatg tgaa
//
