LOCUS       NM_006836               8681 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens GCN1 activator of EIF2AK4 (GCN1), mRNA.
ACCESSION   NM_006836 XM_045792
VERSION     NM_006836.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Tatara,Y., Kasai,S., Kokubu,D., Tsujita,T., Mimura,J. and Itoh,K.
  TITLE     Emerging Role of GCN1 in Disease and Homeostasis
  JOURNAL   Int J Mol Sci 25 (5), 2998 (2024)
   PUBMED   38474243
  REMARK    GeneRIF: Emerging Role of GCN1 in Disease and Homeostasis.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Zhao,S., Cordes,J., Caban,K.M., Gotz,M.J., Mackens-Kiani,T.,
            Veltri,A.J., Sinha,N.K., Weickert,P., Kaya,S., Hewitt,G.,
            Nedialkova,D.D., Frohlich,T., Beckmann,R., Buskirk,A.R., Green,R.
            and Stingele,J.
  TITLE     RNF14-dependent atypical ubiquitylation promotes
            translation-coupled resolution of RNA-protein crosslinks
  JOURNAL   Mol Cell 83 (23), 4290-4303 (2023)
   PUBMED   37951216
REFERENCE   3  (bases 1 to 8681)
  AUTHORS   Suryo Rahmanto,A., Blum,C.J., Scalera,C., Heidelberger,J.B.,
            Mesitov,M., Horn-Ghetko,D., Graf,J.F., Mikicic,I., Hobrecht,R.,
            Orekhova,A., Ostermaier,M., Ebersberger,S., Mockel,M.M.,
            Krapoth,N., Da Silva Fernandes,N., Mizi,A., Zhu,Y., Chen,J.X.,
            Choudhary,C., Papantonis,A., Ulrich,H.D., Schulman,B.A., Konig,J.
            and Beli,P.
  TITLE     K6-linked ubiquitylation marks formaldehyde-induced RNA-protein
            crosslinks for resolution
  JOURNAL   Mol Cell 83 (23), 4272-4289 (2023)
   PUBMED   37951215
REFERENCE   4  (bases 1 to 8681)
  AUTHORS   Gurzeler,L.A., Link,M., Ibig,Y., Schmidt,I., Galuba,O.,
            Schoenbett,J., Gasser-Didierlaurant,C., Parker,C.N., Mao,X.,
            Bitsch,F., Schirle,M., Couttet,P., Sigoillot,F., Ziegelmuller,J.,
            Uldry,A.C., Teodorowicz,W., Schmiedeberg,N., Muhlemann,O. and
            Reinhardt,J.
  TITLE     Drug-induced eRF1 degradation promotes readthrough and reveals a
            new branch of ribosome quality control
  JOURNAL   Cell Rep 42 (9), 113056 (2023)
   PUBMED   37651229
  REMARK    Erratum:[Cell Rep. 2023 Sep 26;42(9):113159. doi:
            10.1016/j.celrep.2023.113159.. PMID: 37708027]
REFERENCE   5  (bases 1 to 8681)
  AUTHORS   Oltion,K., Carelli,J.D., Yang,T., See,S.K., Wang,H.Y., Kampmann,M.
            and Taunton,J.
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of
            translation factors on stalled ribosomes
  JOURNAL   Cell 186 (2), 346-362 (2023)
   PUBMED   36638793
  REMARK    GeneRIF: An E3 ligase network engages GCN1 to promote the
            degradation of translation factors on stalled ribosomes.
REFERENCE   6  (bases 1 to 8681)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 8681)
  AUTHORS   Scherer,S.E., Muzny,D.M., Buhay,C.J., Chen,R., Cree,A., Ding,Y.,
            Dugan-Rocha,S., Gill,R., Gunaratne,P., Harris,R.A., Hawes,A.C.,
            Hernandez,J., Hodgson,A.V., Hume,J., Jackson,A., Khan,Z.M.,
            Kovar-Smith,C., Lewis,L.R., Lozado,R.J., Metzker,M.L.,
            Milosavljevic,A., Miner,G.R., Montgomery,K.T., Morgan,M.B.,
            Nazareth,L.V., Scott,G., Sodergren,E., Song,X.Z., Steffen,D.,
            Lovering,R.C., Wheeler,D.A., Worley,K.C., Yuan,Y., Zhang,Z.,
            Adams,C.Q., Ansari-Lari,M.A., Ayele,M., Brown,M.J., Chen,G.,
            Chen,Z., Clerc-Blankenburg,K.P., Davis,C., Delgado,O., Dinh,H.H.,
            Draper,H., Gonzalez-Garay,M.L., Havlak,P., Jackson,L.R.,
            Jacob,L.S., Kelly,S.H., Li,L., Li,Z., Liu,J., Liu,W., Lu,J.,
            Maheshwari,M., Nguyen,B.V., Okwuonu,G.O., Pasternak,S., Perez,L.M.,
            Plopper,F.J., Santibanez,J., Shen,H., Tabor,P.E., Verduzco,D.,
            Waldron,L., Wang,Q., Williams,G.A., Zhang,J., Zhou,J., Allen,C.C.,
            Amin,A.G., Anyalebechi,V., Bailey,M., Barbaria,J.A., Bimage,K.E.,
            Bryant,N.P., Burch,P.E., Burkett,C.E., Burrell,K.L., Calderon,E.,
            Cardenas,V., Carter,K., Casias,K., Cavazos,I., Cavazos,S.R.,
            Ceasar,H., Chacko,J., Chan,S.N., Chavez,D., Christopoulos,C.,
            Chu,J., Cockrell,R., Cox,C.D., Dang,M., Dathorne,S.R., David,R.,
            Davis,C.M., Davy-Carroll,L., Deshazo,D.R., Donlin,J.E., D'Souza,L.,
            Eaves,K.A., Simons,R., Emery-Cohen,A.J., Escotto,M., Flagg,N.,
            Forbes,L.D., Gabisi,A.M., Garza,M., Hamilton,C., Henderson,N.,
            Hernandez,O., Hines,S., Hogues,M.E., Huang,M., Idlebird,D.G.,
            Johnson,R., Jolivet,A., Jones,S., Kagan,R., King,L.M., Leal,B.,
            Lebow,H., Lee,S., LeVan,J.M., Lewis,L.C., London,P.,
            Lorensuhewa,L.M., Loulseged,H., Lovett,D.A., Lucier,A.,
            Lucier,R.L., Ma,J., Madu,R.C., Mapua,P., Martindale,A.D.,
            Martinez,E., Massey,E., Mawhiney,S., Meador,M.G., Mendez,S.,
            Mercado,C., Mercado,I.C., Merritt,C.E., Miner,Z.L., Minja,E.,
            Mitchell,T., Mohabbat,F., Mohabbat,K., Montgomery,B., Moore,N.,
            Morris,S., Munidasa,M., Ngo,R.N., Nguyen,N.B., Nickerson,E.,
            Nwaokelemeh,O.O., Nwokenkwo,S., Obregon,M., Oguh,M., Oragunye,N.,
            Oviedo,R.J., Parish,B.J., Parker,D.N., Parrish,J., Parks,K.L.,
            Paul,H.A., Payton,B.A., Perez,A., Perrin,W., Pickens,A.,
            Primus,E.L., Pu,L.L., Puazo,M., Quiles,M.M., Quiroz,J.B.,
            Rabata,D., Reeves,K., Ruiz,S.J., Shao,H., Sisson,I., Sonaike,T.,
            Sorelle,R.P., Sutton,A.E., Svatek,A.F., Svetz,L.A., Tamerisa,K.S.,
            Taylor,T.R., Teague,B., Thomas,N., Thorn,R.D., Trejos,Z.Y.,
            Trevino,B.K., Ukegbu,O.N., Urban,J.B., Vasquez,L.I., Vera,V.A.,
            Villasana,D.M., Wang,L., Ward-Moore,S., Warren,J.T., Wei,X.,
            White,F., Williamson,A.L., Wleczyk,R., Wooden,H.S., Wooden,S.H.,
            Yen,J., Yoon,L., Yoon,V., Zorrilla,S.E., Nelson,D.,
            Kucherlapati,R., Weinstock,G. and Gibbs,R.A.
  CONSRTM   Baylor College of Medicine Human Genome Sequencing Center Sequence
            Production Team
  TITLE     The finished DNA sequence of human chromosome 12
  JOURNAL   Nature 440 (7082), 346-351 (2006)
   PUBMED   16541075
REFERENCE   8  (bases 1 to 8681)
  AUTHORS   Andersen,J.S., Lam,Y.W., Leung,A.K., Ong,S.E., Lyon,C.E.,
            Lamond,A.I. and Mann,M.
  TITLE     Nucleolar proteome dynamics
  JOURNAL   Nature 433 (7021), 77-83 (2005)
   PUBMED   15635413
REFERENCE   9  (bases 1 to 8681)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
REFERENCE   10 (bases 1 to 8681)
  AUTHORS   Marton,M.J., Vazquez de Aldana,C.R., Qiu,H., Chakraburtty,K. and
            Hinnebusch,A.G.
  TITLE     Evidence that GCN1 and GCN20, translational regulators of GCN4,
            function on elongating ribosomes in activation of eIF2alpha kinase
            GCN2
  JOURNAL   Mol Cell Biol 17 (8), 4474-4489 (1997)
   PUBMED   9234705
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC004263.1, D86973.2, U88837.1, BQ929459.1, BU186578.1, BC021129.1
            and AC004812.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006836.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC153881.1, BK006575.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300648.7/ ENSP00000300648.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AC004263.1         2430-2434           c
            6-891               D86973.2           1-886
            892-1025            U88837.1           866-999
            1026-1478           D86973.2           1021-1473
            1479-1509           BQ929459.1         574-604
            1510-3953           D86973.2           1505-3948
            3954-4259           BU186578.1         408-713
            4260-8613           D86973.2           4255-8608
            8614-8673           BC021129.1         1481-1540
            8674-8681           AC004812.1         73254-73261         c
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.23"
     gene            1..8681
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /note="GCN1 activator of EIF2AK4"
                     /db_xref="GeneID:10985"
                     /db_xref="HGNC:HGNC:4199"
                     /db_xref="MIM:605614"
     exon            1..36
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..8034
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /note="GCN1, eIF2 alpha kinase activator homolog; GCN1
                     (general control of amino-acid synthesis 1, yeast)-like 1;
                     translational activator GCN1; peroxisome proliferator
                     activated receptor interacting complex protein; hsGCN1;
                     GCN1-like protein 1; GCN1 general control of amino-acid
                     synthesis 1-like 1; GCN1 eIF-2-alpha kinase activator
                     homolog; general control of amino-acid synthesis 1-like
                     protein 1; eIF-2-alpha kinase activator GCN1"
                     /codon_start=1
                     /product="stalled ribosome sensor GCN1"
                     /protein_id="NP_006827.1"
                     /db_xref="CCDS:CCDS41847.1"
                     /db_xref="GeneID:10985"
                     /db_xref="HGNC:HGNC:4199"
                     /db_xref="MIM:605614"
                     /translation="MAADTQVSETLKRFAGKVTTASVKERREILSELGKCVAGKDLPE
                     GAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGIGSKA
                     GVPSKSSGSAALLALTWTCLLVRIVFPSRAKRQGDIWNKLVEVQCLLLLEVLGGSHKH
                     AVDGAVKKLTKLWKENPGLVEQYLSAILSLEPNQNYAGMLGLLVQFCTSHKEMDVVSQ
                     HKSALLDFYMKNILMSKVKPPKYLLDSCAPLLRYLSHSEFKDLILPTIQKSLLRSPEN
                     VIETISSLLASVTLDLSQYAMDIVKGLAGHLKSNSPRLMDEAVLALRNLARQCSDSSA
                     MESLTKHLFAILGGSEGKLTVVAQKMSVLSGIGSVSHHVVSGPSSQVLNGIVAELFIP
                     FLQQEVHEGTLVHAVSVLALWCNRFTMEVPKKLTEWFKKAFSLKTSTSAVRHAYLQCM
                     LASYRGDTLLQALDLLPLLIQTVEKAASQSTQVPTITEGVAAALLLLKLSVADSQAEA
                     KLSSFWQLIVDEKKQVFTSEKFLVMASEDALCTVLHLTERLFLDHPHRLTGNKVQQYH
                     RALVAVLLSRTWHVRRQAQQTVRKLLSSLGGFKLAHGLLEELKTVLSSHKVLPLEALV
                     TDAGEVTEAGKAYVPPRVLQEALCVISGVPGLKGDVTDTEQLAQEMLIISHHPSLVAV
                     QSGLWPALLARMKIDPEAFITRHLDQIIPRMTTQSPLNQSSMNAMGSLSVLSPDRVLP
                     QLISTITASVQNPALRLVTREEFAIMQTPAGELYDKSIIQSAQQDSIKKANMKRENKA
                     YSFKEQIIELELKEEIKKKKGIKEEVQLTSKQKEMLQAQLDREAQVRRRLQELDGELE
                     AALGLLDIILAKNPSGLTQYIPVLVDSFLPLLKSPLAAPRIKNPFLSLAACVMPSRLK
                     ALGTLVSHVTLRLLKPECVLDKSWCQEELSVAVKRAVMLLHTHTITSRVGKGEPGAAP
                     LSAPAFSLVFPFLKMVLTEMPHHSEEEEEWMAQILQILTVQAQLRASPNTPPGRVDEN
                     GPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTTLCASSSGDDGCAFAEQEEVDVLL
                     CALQSPCASVRETVLRGLMELHMVLPAPDTDEKNGLNLLRRLWVVKFDKEEEIRKLAE
                     RLWSMMGLDLQPDLCSLLIDDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEI
                     YQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVP
                     DALNDRHPDVRKCMLDAALATLNTHGKENVNSLLPVFEEFLKNAPNDASYDAVRQSVV
                     VLMGSLAKHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMI
                     QRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRRE
                     GALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHG
                     VKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHV
                     KVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDA
                     PSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLD
                     PVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAG
                     LGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILK
                     ALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDL
                     LFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVERRNRVLAGLYMGRSDTQLVV
                     RQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGE
                     KILPEIIPILEEGLRSQKSDERQGVCIGLSEIMKSTSRDAVLYFSESLVPTARKALCD
                     PLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAIKSR
                     VVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLE
                     MANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNIYCSRSKADYTSHL
                     RSLVSGLIRLFNDSSPVVLEESWDALNAITKKLDAGNQLALIEELHKEIRLIGNESKG
                     EHVPGFCLPKKGVTSILPVLREGVLTGSPEQKEEAAKALGLVIRLTSADALRPSVVSI
                     TGPLIRILGDRFSWNVKAALLETLSLLLAKVGIALKPFLPQLQTTFTKALQDSNRGVR
                     LKAADALGKLISIHIKVDPLFTELLNGIRAMEDPGVRDTMLQALRFVIQGAGAKVDAV
                     IRKNIVSLLLSMLGHDEDNTRISSAGCLGELCAFLTEEELSAVLQQCLLADVSGIDWM
                     VRHGRSLALSVAVNVAPGRLCAGRYSSDVQEMILSSATADRIPIAVSGVRGMGFLMRH
                     HIETGGGQLPAKLSSLFVKCLQNPSSDIRLVAEKMIWWANKDPLPPLDPQAIKPILKA
                     LLDNTKDKNTVVRAYSDQAIVNLLKMRQGEEVFQSLSKILDVASLEVLNEVNRRSLKK
                     LASQADSTEQVDDTILT"
     exon            37..139
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            140..203
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            204..335
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            336..444
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            445..543
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            544..678
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            679..747
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            748..856
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            857..931
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1060
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1111
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1210
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1384
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1537
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1630
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1706
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1866
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..2056
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2181
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2360
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2454
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2568
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..2767
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..2923
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            2924..3105
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3106..3186
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3187..3330
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3458
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3648
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3649..3719
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3720..3885
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            3886..4080
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            4081..4327
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            4328..4449
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4564
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            4565..4744
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            4745..4965
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            4966..5034
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            5035..5173
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            5174..5370
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            5371..5513
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            5514..5631
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            5632..5847
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            5848..6012
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            6013..6174
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            6175..6267
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            6268..6411
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            6412..6681
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            6682..6795
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            6796..7026
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7027..7220
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7221..7335
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7336..7432
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7433..7581
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7582..7689
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7690..7908
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     exon            7909..8681
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8591..8596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /note="hexamer: AATAAA"
     polyA_site      8615
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /note="major polyA site"
     regulatory      8645..8650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
                     /note="hexamer: ATTAAA"
     polyA_site      8681
                     /gene="GCN1"
                     /gene_synonym="GCN1L; GCN1L1; PRIC295"
ORIGIN      
        1 ggagtcagcc ccggcaggat ggcggcggac acgcaggttt ccgagacact aaagcgtttt
       61 gcagggaagg tgacaacagc cagtgtaaag gaacggagag aaatcctcag tgaacttggg
      121 aagtgtgttg ctggaaaaga tcttccagag ggagcagtga aggggctctg caaattgttc
      181 tgcttgactc tgcatcgata tagagatgca gcctcccgca gggccttgca ggcagccatc
      241 cagcagttgg ctgaggccca gccagaagcc actgctaaga accttctaca ctctctgcag
      301 tcttctggta taggctccaa agcaggtgtt cccagtaaga gcagtggctc tgccgccttg
      361 ctggccttga cctggacctg cctcctggtg cgcattgtct ttccatcgag agccaagcga
      421 caaggagaca tctggaacaa actggtggaa gtgcagtgcc tgctcttgct ggaggtgctg
      481 ggtggctccc acaagcacgc cgtggatggt gctgtgaaga aactcacgaa gctgtggaaa
      541 gagaaccccg ggctggtgga acagtacttg tcagccattc tcagcctaga gcccaaccag
      601 aactatgctg gcatgctggg gctgctggtg cagttctgca cgagtcacaa ggagatggac
      661 gtggtcagtc agcacaagag cgccctactg gacttttaca tgaagaacat cctgatgagc
      721 aaagtcaagc ctccgaagta cctgttggat agctgtgccc ctctgctccg atacctgtcc
      781 cactcagaat ttaaggatct gatactgccc accatacaga agtccttact gaggagtcca
      841 gagaatgtta ttgaaactat ttctagtctg ctggcatcag tgacgcttga cctcagccag
      901 tatgccatgg acatcgtgaa aggactggct ggtcacctga aatccaacag tccccgcctg
      961 atggatgaag ctgtgctggc actgcggaac ctggcacgcc agtgcagtga ctcttcggcc
     1021 atggaatccc tgaccaagca cctatttgct atcctcggag gctcggaagg aaaactaact
     1081 gttgtagccc agaagatgag cgtcctctca gggattggga gcgtcagtca tcacgtggtg
     1141 tctggacctt ccagtcaggt cctgaatggg atcgtggctg agctgttcat cccgttcctt
     1201 cagcaggaag ttcatgaagg gaccttggta cacgctgtct cagtcctggc tctctggtgt
     1261 aaccgattca ctatggaagt gcccaagaag ctcactgaat ggttcaaaaa agctttcagc
     1321 cttaaaacct ccacatctgc ggtgaggcat gcctacctgc agtgcatgtt ggcctcttac
     1381 cggggtgaca cgctgttgca ggccctggac ttactgccct tgctcatcca gacagtggag
     1441 aaggcagcct cccaaagcac tcaggttccc accatcactg aaggggttgc cgcagccttg
     1501 ttgctcttaa agttgtcagt ggctgactca caggctgagg ccaaactgag cagtttctgg
     1561 cagttgattg tggatgagaa aaagcaggtt ttcacttctg agaaattcct ggtcatggct
     1621 tcagaggatg ccctgtgtac tgtgttgcat ctgacagaga gacttttcct tgaccacccg
     1681 catagactca ctggcaacaa agttcagcag taccaccggg ctctggtggc ggtgctcctg
     1741 agccgcacct ggcacgtccg caggcaggct cagcagacag ttcggaagct gctgtcctct
     1801 cttgggggct ttaagctggc gcacggactc ttggaggagc tgaagactgt cctcagttct
     1861 cacaaggtgc tgcccttaga ggctttggtg actgatgctg gagaggtgac tgaggcaggc
     1921 aaggcctacg tgcctccacg ggtcctgcag gaggctctgt gtgtcatctc cggtgtgcca
     1981 gggctcaagg gtgatgtcac cgacactgaa caactggccc aggaaatgct gatcatctcc
     2041 caccacccat ccttagttgc cgtgcagtct ggactttggc cagcacttct tgccaggatg
     2101 aagatcgatc ctgaagcctt tatcaccagg cacctggatc agatcattcc caggatgacc
     2161 acacagagtc ccctaaacca gtcctccatg aatgccatgg gctccctttc cgtcctgtcg
     2221 ccggaccggg tcctcccaca gctcatcagc accatcactg cctccgtgca gaaccctgca
     2281 ctgcgcctgg tgacgcggga ggagtttgcc attatgcaga cccctgctgg ggagctgtat
     2341 gacaaatcca tcattcagag tgcccagcag gacagcataa aaaaggccaa catgaagcga
     2401 gagaacaaag cttattcctt caaagagcag atcatcgagc tggagctgaa ggaggagata
     2461 aagaagaaga aaggcatcaa agaggaggtg cagctgacca gcaagcagaa ggagatgctg
     2521 caggcccagc tagacaggga ggcgcaggtc cggaggcggc tgcaggagct ggatggggag
     2581 ctggaggcgg cgcttggact gctggacatc atcctggcca agaacccgtc cggcctgacc
     2641 cagtacatcc ctgttttggt cgactctttt ctgcccttgc tgaagtctcc cctggctgct
     2701 cccaggatca agaacccctt cttgtccttg gctgcctgtg tcatgccctc taggctcaag
     2761 gctttgggca ctttggtgag ccacgtgacc ctgcgcctgc tgaagccaga gtgtgtcctg
     2821 gataagtcct ggtgccagga agagctgtcg gtggctgtga agagggcggt gatgctgctg
     2881 cacacccaca ccatcaccag cagggtgggc aagggggagc caggtgctgc gcccttgtcc
     2941 gcgccagcct tctccttagt cttcccgttt ctgaagatgg tgctgacgga gatgccccac
     3001 cacagtgagg aggaggagga gtggatggcc cagattcttc agatcctcac tgtccaagcc
     3061 cagctgaggg cctcccccaa caccccaccc gggcgggtgg acgagaatgg cccggagttg
     3121 ctgcctcgcg tggccatgct gcgtcttctg acttgggtga tcgggacggg ctcgcctcgc
     3181 ttacaggttc tggcttcaga caccctgacc accctgtgtg ccagcagcag tggtgatgat
     3241 ggctgtgcct ttgcagagca ggaggaggtg gacgtgctgc tctgtgcctt gcagtccccg
     3301 tgtgccagcg tgcgggaaac cgtgctccgg gggctgatgg aactccacat ggtattgcca
     3361 gcacctgata ctgatgagaa gaatggcctg aaccttctgc ggagactctg ggtggtcaag
     3421 tttgacaagg aggaggagat ccggaagctg gctgagaggc tctggtcaat gatgggccta
     3481 gacctgcagc cagacctctg ctccttgctg attgacgacg tgatctatca tgaggcggct
     3541 gtaaggcagg caggggccga agccctctcc caagcagtgg cacgttacca gcggcaggcg
     3601 gcggaggtta tgggcaggct catggagatt taccaggaaa agctctaccg gccgccccca
     3661 gtgctggatg ctttgggacg agttatttca gaatctcctc cagatcagtg ggaagccagg
     3721 tgtggcttgg cgttggccct caacaagctc tcccagtatt tggacagctc tcaggtgaag
     3781 ccactctttc agttttttgt ccctgatgcc ctcaatgacc gacacccaga tgtccggaag
     3841 tgcatgttgg atgcagccct cgcaacgctc aacactcatg ggaaggagaa cgtcaactcg
     3901 ctgttgccag tattcgagga gttcctgaag aacgcgccca atgatgccag ctatgatgct
     3961 gtgcgacaga gtgtggtggt cctgatgggc tctctggcca agcacctgga caagagtgac
     4021 cccaaagtga agcccattgt tgccaagctc atcgctgccc tctccacccc ctcccagcag
     4081 gtccaggagt ccgtagccag ctgcttgcca ccccttgtgc cagccatcaa ggaggatgct
     4141 ggagggatga tccagaggct tatgcagcag ctgctggagt cagacaagta cgcagagcgc
     4201 aaaggggccg cctatggcct ggcgggcctg gtgaagggcc tgggcatcct ctcgctgaag
     4261 caacaggaga tgatggcggc actgactgat gccatccaag ataagaagaa cttccgccgg
     4321 cgagagggag ccctctttgc cttcgagatg ctctgcacca tgctggggaa actttttgag
     4381 ccgtatgtgg ttcacgtgct gccccatctg ctcctgtgct ttggggatgg aaaccagtat
     4441 gtgcgtgagg ctgcagatga ctgtgccaag gctgtgatga gcaacttgag tgctcacggg
     4501 gtgaagctgg tgctcccctc cttactggct gccctggagg aggaatcgtg gcggaccaaa
     4561 gctgggtcag tggagcttct tggggcaatg gcgtactgtg ctcctaagca gctgtcatcc
     4621 tgtctaccca acattgtgcc caagcttacg gaggtgctga ccgactccca tgtcaaagtc
     4681 cagaaggctg gacagcaggc gctcaggcag atcggctccg ttatcaggaa cccggagatc
     4741 ctggccattg ctccagtcct cctggatgcc ctgacggatc cctccaggaa gacccagaag
     4801 tgcttgcaga ccctgctgga caccaagttt gtccacttca ttgatgcccc atccctggcc
     4861 ctcatcatgc ccattgtcca gagagccttc caggaccgtt ccacggacac gcggaagatg
     4921 gcagcccaga ttattggcaa catgtactcc ctgacagacc agaaggactt ggctccgtac
     4981 ctgcccagcg tgacgcctgg cctgaaagca tcgcttttgg accctgtgcc tgaggtgcgg
     5041 accgtatctg caaaggccct tggggccatg gtgaagggca tgggggagtc gtgctttgag
     5101 gacttgctgc cgtggctgat ggagacactg acctatgagc agagctctgt ggatcgctca
     5161 ggcgctgcac aggggttggc tgaggtcatg gccggtttgg gggtggagaa gttggagaag
     5221 ttgatgccag aaatcgtggc tacagccagc aaagtggaca ttgcacccca tgtccgagat
     5281 ggctacatta tgatgtttaa ctacctgccc atcacctttg gagacaagtt tactccttat
     5341 gtggggccca tcatcccctg tatcctcaaa gctcttgctg atgagaatga gtttgtgcgt
     5401 gacaccgccc tgcgcgcggg ccagcgggtt atctccatgt acgctgagac agccatcgcc
     5461 ctgctgctgc cccagctaga gcaaggcctc tttgatgacc tttggagaat caggttcagc
     5521 tctgttcagc tccttgggga tctcctgttt cacatctcag gagtcactgg gaagatgacc
     5581 acagaaactg cctctgagga tgataacttt ggaactgccc agtccaacaa ggcgatcatc
     5641 actgccctgg gggtagagcg gcggaaccgg gtgttggcag ggctgtacat gggccgctca
     5701 gacacccagc tggtggtgcg gcaggcgtcc ctgcatgtct ggaagattgt tgtctccaat
     5761 accccccgca ccttgcgtga gatcctaccc actctctttg ggctcctgct gggtttcctg
     5821 gccagcacgt gtgcagataa gagaacgatt gcagcgagaa cattgggaga tcttgtgcgg
     5881 aagttagggg agaaaatcct ccccgagatc atccccatcc ttgaggaagg cctgaggtct
     5941 cagaagagcg atgagaggca gggtgtgtgc attggcctaa gtgagatcat gaagtccacc
     6001 agccgggatg ccgtgctgta tttctctgaa tccctcgtgc ccacggcaag gaaggctttg
     6061 tgtgacccac tggaggaggt cagagaggcg gcagccaaga ctttcgagca gctgcattcc
     6121 accatcggcc accaggctct ggaggacatt ctcccatttt tactaaagca gctggatgac
     6181 gaggaggtgt cagagtttgc cttggatggt ctgaagcaag tcatggctat taagagtcgt
     6241 gtggtgctgc cctaccttgt gcccaagctg acaacgccac ctgtcaacac ccgggtgctg
     6301 gctttccttt cgtcagtggc tggtgatgcc ctcacccgtc atcttggcgt gatcctccca
     6361 gcggtcatgc tggccctgaa ggaaaagctt gggaccccag atgagcagct ggagatggcc
     6421 aattgtcagg ctgtgatcct ctccgtagag gatgacacag ggcaccggat catcatcgag
     6481 gatctgctgg aggccacccg cagccctgag gtgggcatga ggcaagctgc tgccatcatc
     6541 ctcaacatct actgttcccg ctcaaaggct gactacacca gccacctgcg gagcctggtc
     6601 tcgggcctga tccgcctctt caatgactcc agccctgtgg ttctggagga gagctgggat
     6661 gccctaaatg ccatcactaa gaagctggat gctggcaacc agttggcact cattgaagag
     6721 ctgcacaagg aaatccggct catagggaac gagagcaaag gcgagcatgt gccaggattc
     6781 tgcctcccga agaagggagt gacctccatc cttccagtgt tgcgggaagg agtcctgact
     6841 ggcagccctg agcagaagga ggaggcagcc aaagccttag gcttggtaat ccgcctgacc
     6901 tcggctgacg ccctgaggcc ctccgtggtc agcatcactg gccctctgat ccgcatcctg
     6961 ggggacaggt tcagctggaa tgtgaaggcg gctctgctcg agacactcag cctcttgttg
     7021 gctaaggttg ggattgccct gaagcccttc ctgccccagc tgcagaccac tttcaccaaa
     7081 gccctgcagg actccaaccg gggggtgcgc ctgaaggccg cagatgctct ggggaagctc
     7141 atttccatcc acattaaggt ggaccccctc ttcacagagc tgctcaatgg catccgcgcc
     7201 atggaggacc caggtgtcag ggacaccatg ctgcaggccc tgaggtttgt gattcaggga
     7261 gcaggggcca aagtggatgc cgtcatccgg aaaaacatcg tctcactcct gctgagcatg
     7321 ctgggacacg atgaggacaa cactcgcatc tcctcagccg ggtgcctagg ggaactgtgt
     7381 gcctttttga ctgaagagga gcttagtgcc gttctacagc agtgcttgct ggcggacgtg
     7441 tccggcattg actggatggt tcggcacggg cggagcctgg cactttccgt ggctgtgaat
     7501 gtggctcctg gcagactttg tgccggcaga tatagcagtg atgttcagga aatgatcctg
     7561 agcagtgcca cggcggacag gatccccatt gcggtgagcg gggtccgggg catgggcttt
     7621 ctcatgagac accacatcga gacaggcgga gggcagttgc cggccaaact ttccagcctg
     7681 ttcgttaagt gtctgcagaa cccatccagc gacatcaggc tggtggctga gaagatgatc
     7741 tggtgggcaa ataaggaccc actgcctccc ctggaccccc aggccatcaa gcccatcctg
     7801 aaggctcttc ttgacaacac caaggataag aacaccgtgg tcagggccta cagcgaccag
     7861 gcaattgtca acctcctcaa gatgcggcag ggtgaagagg tgtttcagtc cctctccaag
     7921 atcctggatg tggccagttt ggaggtgctg aacgaggtta accgaaggtc cctgaagaag
     7981 ctggccagcc aggccgactc cacggagcag gtggacgaca ccatcctgac atgagaggcc
     8041 tgggccagca gcagcattgc cgctccacat ctttgctcaa tgttttcatt tttgaaaata
     8101 catttgttcc aatggggagc ttggaagatg gcgttcccag aaagtatttt aatatcaata
     8161 gaccacagcc aaagccttaa atcaaaccca cacacaactg aaaattgcct cctccatctc
     8221 tcaccttttc ctgtggagaa gagaaggaaa agcacacgca tgcgcctcag caaatggcag
     8281 cccaggagct gtttgtccag tttagcatgg ctaggtctgg aactataata gcagggtcag
     8341 actgtgggtt cctcttctcc tgtgcttgag ctctggtttg agagctggcg ctaccaacct
     8401 ttttcctata tcccgagtgg ggcacagacg gtggatctct gcccagtgtg gtgtgtctgg
     8461 cttggctttt caatattgtg aggtctgaat ggatctgacc cctgtcagat gaaaatgatt
     8521 cacagctctg gcagttccca agtctgggga ggggtatagg tttgaaaggc tgtttgaaag
     8581 aggaatgttt aataaaggct ttgatttaat cttgatataa acagattttt aaaaatctcc
     8641 acccattaaa catgaagctt cctgcatcag gaataaggaa a
//
