LOCUS       NM_001410845            4253 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2),
            transcript variant 4, mRNA.
ACCESSION   NM_001410845 XM_047445614
VERSION     NM_001410845.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Yang,C., Yin,L., Zhou,P., Liu,X., Yang,M., Yang,F., Jiang,H. and
            Ding,K.
  TITLE     Transcriptional regulation of IER5 in response to radiation in
            HepG2
  JOURNAL   Cancer Gene Ther 23 (2-3), 61-65 (2016)
   PUBMED   26915404
  REMARK    GeneRIF: Authors propose the GCF regulated transcriptional
            activity, at least in part, contributed to the upregulation of IER5
            on radiation in HepG2 cells. The present findings provide insights
            into understanding the regulatory mechanisms of IER5.
REFERENCE   3  (bases 1 to 4253)
  AUTHORS   Yoshimoto,R., Okawa,K., Yoshida,M., Ohno,M. and Kataoka,N.
  TITLE     Identification of a novel component C2ORF3 in the lariat-intron
            complex: lack of C2ORF3 interferes with pre-mRNA splicing via
            intron turnover pathway
  JOURNAL   Genes Cells 19 (1), 78-87 (2014)
   PUBMED   24304693
  REMARK    GeneRIF: C2ORF3 protein is required for pre-mRNA splicing through
            its presumable role in efficient intron turnover
REFERENCE   4  (bases 1 to 4253)
  AUTHORS   Melville,S.A., Buros,J., Parrado,A.R., Vardarajan,B., Logue,M.W.,
            Shen,L., Risacher,S.L., Kim,S., Jun,G., DeCarli,C., Lunetta,K.L.,
            Baldwin,C.T., Saykin,A.J. and Farrer,L.A.
  CONSRTM   Alzheimer's Disease Neuroimaging Initiative
  TITLE     Multiple loci influencing hippocampal degeneration identified by
            genome scan
  JOURNAL   Ann Neurol 72 (1), 65-75 (2012)
   PUBMED   22745009
REFERENCE   5  (bases 1 to 4253)
  AUTHORS   Paracchini,S., Ang,Q.W., Stanley,F.J., Monaco,A.P., Pennell,C.E.
            and Whitehouse,A.J.
  TITLE     Analysis of dyslexia candidate genes in the Raine cohort
            representing the general Australian population
  JOURNAL   Genes Brain Behav 10 (2), 158-165 (2011)
   PUBMED   20846247
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 4253)
  AUTHORS   Takimoto,M., Mao,P., Wei,G., Yamazaki,H., Miura,T., Johnson,A.C.
            and Kuzumaki,N.
  TITLE     Molecular analysis of the GCF gene identifies revisions to the cDNA
            and amino acid sequences(1)
  JOURNAL   Biochim Biophys Acta 1447 (1), 125-131 (1999)
   PUBMED   10500253
REFERENCE   7  (bases 1 to 4253)
  AUTHORS   Mao,P.
  TITLE     [Revisions of the cDNA and primary protein structure of human
            transcription factor GCF]
  JOURNAL   Hokkaido Igaku Zasshi 74 (4), 315-330 (1999)
   PUBMED   10480038
REFERENCE   8  (bases 1 to 4253)
  AUTHORS   Reed,A.L., Yamazaki,H., Kaufman,J.D., Rubinstein,Y., Murphy,B. and
            Johnson,A.C.
  TITLE     Molecular cloning and characterization of a transcription regulator
            with homology to GC-binding factor
  JOURNAL   J Biol Chem 273 (34), 21594-21602 (1998)
   PUBMED   9705290
REFERENCE   9  (bases 1 to 4253)
  AUTHORS   Johnson,A.C., Kageyama,R., Popescu,N.C. and Pastan,I.
  TITLE     Expression and chromosomal localization of the gene for the human
            transcriptional repressor GCF
  JOURNAL   J Biol Chem 267 (3), 1689-1694 (1992)
   PUBMED   1370479
REFERENCE   10 (bases 1 to 4253)
  AUTHORS   Kageyama,R. and Pastan,I.
  TITLE     Molecular cloning and characterization of a human DNA binding
            factor that represses transcription
  JOURNAL   Cell 59 (5), 815-825 (1989)
   PUBMED   2556218
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC005034.2.
            
            On Aug 16, 2022 this sequence version replaced XM_047445614.1.
            
            Summary: The first mRNA transcript isolated for this gene was part
            of an artificial chimera derived from two distinct gene transcripts
            and a primer used in the cloning process (see Genbank accession
            M29204). A positively charged amino terminus present only in the
            chimera was determined to bind GC-rich DNA, thus mistakenly thought
            to identify a transcription factor gene. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2896218.1,
                                           SRR18074967.3874675.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               AC005034.2         74783-75107
            326-454             AC005034.2         79047-79175
            455-565             AC005034.2         83275-83385
            566-663             AC005034.2         84411-84508
            664-779             AC005034.2         89383-89498
            780-966             AC005034.2         91271-91457
            967-1090            AC005034.2         93598-93721
            1091-1172           AC005034.2         94979-95060
            1173-1285           AC005034.2         95617-95729
            1286-1485           AC005034.2         96473-96672
            1486-1636           AC005034.2         97721-97871
            1637-1758           AC005034.2         105384-105505
            1759-1835           AC005034.2         112178-112254
            1836-1902           AC005034.2         113682-113748
            1903-2049           AC005034.2         115414-115560
            2050-2174           AC005034.2         119645-119769
            2175-4253           AC005034.2         120915-122993
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p12"
     gene            1..4253
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="GC-rich sequence DNA-binding factor 2"
                     /db_xref="GeneID:6936"
                     /db_xref="HGNC:HGNC:1317"
                     /db_xref="MIM:189901"
     exon            1..325
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..2292
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="isoform 4 is encoded by transcript variant 4; GC
                     binding factor; transcription factor 9 (binds GC-rich
                     sequences); intron Large complex component GCFC2; GC
                     bindng factor; TCF-9"
                     /codon_start=1
                     /product="intron Large complex component GCFC2 isoform 4"
                     /protein_id="NP_001397774.1"
                     /db_xref="CCDS:CCDS92788.1"
                     /db_xref="GeneID:6936"
                     /db_xref="HGNC:HGNC:1317"
                     /db_xref="MIM:189901"
                     /translation="MAHRPKRTFRQRAADSSDSDGAEESPAEPGAPRELPVPGSAEEE
                     PPSGGGRAQVAGLPHRVRGPRGRGRVWASSRRATKAAPRADEGSESRTLDVSTDEEDK
                     IHHSSESKDDQGLSSDSSSSLGEKELSSTVKIPDAAFIQAARRKRELARAQDDYISLD
                     VQHTSSISVSRNEETSEESQEDEKQDTWEQQQMRKAVKIIEERDIDLSCGNGSSKVKK
                     FDTSISFPPVNLEIIKKQLNTRLTLLQETHRSHLREYEKYVQDVKSSKSTIQNLESSS
                     NQALNCKFYKSMKIYVENLIDCLNEKIINIQEIESSMHALLLKQAMTFMKRRQDELKH
                     ESTYLQQLSRKDETSTSGNFSVDEKTQWILEEIESRRTKRRQARVLSGNCNHQEGTSS
                     DDELPSAEMIDFQKSQGDILQKQKKVFEEVQDDFCNIQNILLKFQQWREKFPDSYYEA
                     FISLCIPKLLNPLIRVQLIDWNPLKLESTGLKEMPWFKSVEEFMDSSVEDSKKESSSD
                     KKVLSAIINKTIIPRLTDFVEFLWDPLSTSQTTSLITHCRVILEEHSTCENEVSKSRQ
                     DLLKSIVSRMKKAVEDDVFIPLYPKSAVENKTSPHSKFQERQFWSGLKLFRNILLWNG
                     LLTDDTLQELGLGKLLNRYLIIALLNATPGPDVVKKCNQVAACLPEKWFENSAMRTSI
                     PQLENFIQFLLQSAHKLSRSEFRDEVEEIILILVKIKALNQAESFIGEHHLDHLKSLI
                     KED"
     exon            326..454
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            455..565
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            566..663
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            664..779
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            780..966
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1090
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1172
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1285
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1485
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1636
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1758
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1835
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1902
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2049
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2174
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..4253
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2292..2297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="hexamer: AATAAA"
     polyA_site      2318
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="major polyA site"
     regulatory      2496..2501
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="hexamer: ATTAAA"
     polyA_site      2540
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="major polyA site"
     regulatory      4228..4233
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
                     /note="hexamer: ATTAAA"
     polyA_site      4253
                     /gene="GCFC2"
                     /gene_synonym="C2orf3; DNABF; GCF; TCF9"
ORIGIN      
        1 actgaagctg cggcttgcgg ttcagcgggt tctagggcgc cgggcgctcg ggcctcggcc
       61 atggctcaca ggccgaaaag gacttttcgg cagcgcgcgg ctgattccag cgacagcgat
      121 ggcgccgagg agtcgcctgc tgagcctggg gcgccgaggg aacttccggt cccgggttct
      181 gcggaggaag agccgccctc tggaggaggc cgcgcgcagg tggcgggact gccccaccgg
      241 gttcggggcc ctcgtggccg gggccgggtc tgggcgagct cccggcgtgc caccaaagcg
      301 gctccccgcg cggacgaagg ctcagaatcc agaacccttg atgtgtccac agatgaagag
      361 gataaaatac atcactcctc agaaagtaag gatgatcagg gtttgtcttc tgacagttct
      421 agctctcttg gagaaaaaga actttcatca acagttaaga tcccagatgc agcttttatt
      481 caggcagccc gcagaaaacg tgaattggcc agggcccaag atgactatat ttctttggat
      541 gtacaacata cctcctccat ctctgtaagc agaaatgaag aaacaagtga agaaagtcag
      601 gaagatgaaa agcaagatac ttgggaacaa cagcaaatga ggaaagcagt taaaatcata
      661 gaggaaagag acatagatct ttcctgtggc aatggatctt caaaagtgaa gaaatttgat
      721 acttccattt catttccgcc agtaaattta gaaattataa agaagcaatt aaatactaga
      781 ttaacattac tacaggaaac tcaccgctca cacctgaggg agtatgaaaa atacgtacaa
      841 gatgtcaaaa gctcaaagag taccatccag aacctagaga gttcatcaaa tcaagctcta
      901 aattgtaaat tctataaaag catgaaaatt tatgtggaaa atttaattga ctgccttaat
      961 gaaaagatta tcaacatcca agaaatagaa tcatccatgc atgcactcct tttaaaacaa
     1021 gctatgacct ttatgaaacg caggcaagat gaattaaaac atgaatcaac gtatttacaa
     1081 cagttatcac gcaaagatga gacatccaca agtggaaact tctcagtaga tgaaaaaact
     1141 cagtggattt tagaagagat tgaatctcga aggacaaaaa gaagacaagc aagggtgctt
     1201 tctgggaatt gtaaccatca ggaaggaaca tctagtgatg atgaactgcc ttcagcagag
     1261 atgattgact tccaaaaaag ccaaggtgac attttacaga aacagaagaa agtttttgaa
     1321 gaagtgcaag atgatttttg taacatccag aatattttgt tgaaatttca gcaatggcga
     1381 gaaaagtttc ctgactccta ttatgaagct ttcattagtt tatgcatacc aaagctttta
     1441 aatcccctaa tacgagttca gttgattgat tggaatcctc ttaagttgga atccacaggt
     1501 ttaaaagaga tgccatggtt caaatctgta gaagaattta tggatagcag tgtggaagat
     1561 tcaaagaagg aaagtagttc agataaaaaa gtcttgtctg caatcatcaa caaaacaatt
     1621 attccccgac ttacagactt tgtagaattc ctttgggatc ctttgtcaac ctcacagaca
     1681 acaagtttaa taacacattg cagagtgatt cttgaagaac attccacttg tgaaaatgaa
     1741 gttagtaaaa gcagacagga tttacttaaa tccattgttt caagaatgaa aaaggcagta
     1801 gaagatgatg tttttattcc tctgtatcca aagagtgctg tagaaaacaa aacatcacct
     1861 cattcaaagt tccaagaaag acagttctgg tcaggcctaa agctcttccg caatattctt
     1921 ctttggaatg gactccttac agatgacacc ttgcaagaac taggactagg gaagctgcta
     1981 aatcgttacc ttattatagc acttctcaat gccacacctg ggccagatgt ggttaaaaag
     2041 tgcaaccagg tagcagcatg tctaccagaa aaatggtttg aaaattctgc catgaggaca
     2101 tctattccac agctagaaaa cttcattcag tttttattgc agtctgcaca taaattatct
     2161 agaagtgaat tcagggatga agtcgaagaa ataattctta ttttggtgaa aataaaagct
     2221 ttgaatcaag cagaatcctt cataggagag catcacctag accatcttaa atcactaatt
     2281 aaagaagatt gaataaactt tattggaaaa tgctaaaatt ttaatatagt tacactcagt
     2341 tcctttgttt gagaagaagc tggtgcctct ctcttcttta ttccctgtaa tagaaggtag
     2401 gatttgaaaa aaagcaggac tccacctctg tattcccccg tgctttacct tctggcatca
     2461 tgaaaagctg ccatgattct gtggtgttct aaggaattaa atgcactgga gctttaagag
     2521 ctcaacgtgt ttccctttga ttcttttggc tggtctttca attttcttaa gaggggaact
     2581 ggagaaacct tgctaaaggt tttagcttaa gtgagcatgc tactaagtct tctgtcctga
     2641 accacaataa tatcttttag ttctctttaa ctacctttat ttaaaaataa gtcaatatga
     2701 aaatctcagt tggagtgaat tatttgctaa gtattaaatg attaaggcat gtgccgattg
     2761 tcaaattatc cttcagaaat ctgtaccaat ttaatgtacc aacataagca taagtgagtg
     2821 ccaatgaatc cctaccagca gtagttatta tcctttttaa aaatgcattg aaaatttgaa
     2881 aggtaaaaat cttattttaa tagcgtattt tgtgttggct attagagagt aaacatattg
     2941 gttttggcca tttgtaatta tttctgaaat atccatcttt ttcttatgga ctttaagacc
     3001 tttatatttt taaagatatt gattttttgc ccatgcttgt ggcagaagtt ttccttattt
     3061 gccatttttc ttttattttt tttttaattg tattttttga gacagggtct ggctctgtca
     3121 cccaggctgg agagtagtgg cacaatctcg gctcactgca acctctgcct tctgggctca
     3181 agcaatcctc ccacttcaac ctcccaagta gctgggacta taggcatgcg ccaccatgcc
     3241 cagctgattt ttgtaatttt ttgtagagat ggggtttcgt gacattgccc aggctggtct
     3301 cgaactcttg agctcaagca gtctacccac cttgacctcc caaagtgctg ggattacagg
     3361 cttgagcaac catgtttggc ctttctttta aatttgttga tgttttttga agcattaagt
     3421 cattacttat tagcaagtta ctattttgtt tttttgtcat tggtggtagg actaagaagg
     3481 aaacagatat tttaaatact cacaacatgt gattcatttt ctgataaaat atttgtctaa
     3541 taaaacagag gcaagataat ttaatcaaaa gaaattaaga aaattttcca aaaatgttag
     3601 tttactagta atttttctaa gtctacttat taaataactt aagggaaact gtaaatatat
     3661 tttgcaaatt gttgttcaaa agataattgg aaaaaattga atatattgta ggatttgaat
     3721 atacagccta cttttatgtc acctctacct tcaaaactac atataattta ttttaggagt
     3781 tggatttgtt atgtctgttg cagtgaggac ttgtgattca ctggtatgaa gaattttttg
     3841 atgtttatat tagtaacaaa attatacaag gatagaatct catctagaaa atgcaaacta
     3901 ttgttgaaaa gcagttagca tttccacaca accgctgtcc ccagagataa ctgctgtttt
     3961 cattttaatg ttatcctttt agaccttttc tctatttaca aaaatgttac gtgtttctat
     4021 gtgtacatag ttttgggttt tttttttttt tttacgtaag tggcagacac tttattgcac
     4081 atgaaatttg taattttttt ctatttaagt acacatttgg aaattatcct ctgacttttg
     4141 gatttttaac taaatatgtt ggacgtgttc ctttatatat ttaagttcag cacagaaact
     4201 ttcctacctt tttgtggaag ataaaatatt aaaactgctt tcaaaactac aaa
//
