LOCUS       NM_181453               6909 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2),
            transcript variant 1, mRNA.
ACCESSION   NM_181453 XM_003960914 XM_003960915 XM_003960916 XM_003960917
            XM_003960918 XM_003960919 XM_003960920
VERSION     NM_181453.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Liang,X.H., Sun,H., Hsu,C.W., Nichols,J.G., Vickers,T.A., De
            Hoyos,C.L. and Crooke,S.T.
  TITLE     Golgi-endosome transport mediated by M6PR facilitates release of
            antisense oligonucleotides from endosomes
  JOURNAL   Nucleic Acids Res 48 (3), 1372-1391 (2020)
   PUBMED   31840180
  REMARK    GeneRIF: The co-localization of M6PR and of GCC2 with ASOs is
            influenced by the PS modifications, which have been shown to
            enhance the affinity of ASOs for proteins, suggesting that
            localization of these proteins to LEs is mediated by ASO-protein
            interactions. Reduction of M6PR levels also decreased PS-ASO
            activity in mouse cells and in livers of mice treated
            subcutaneously with PS-ASO, indicating a conserved mechanism.
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Kumar,M., Kaur,S., Nazir,A. and Tripathi,R.K.
  TITLE     HIV-1 Nef binds with human GCC185 protein and regulates mannose 6
            phosphate receptor recycling
  JOURNAL   Biochem Biophys Res Commun 474 (1), 137-145 (2016)
   PUBMED   27105913
  REMARK    GeneRIF: In light of existing report suggesting critical role of
            Nef-GCC185 interaction reveals valuable mechanistic insights
            affecting specific protein transport pathway in docking of late
            endosome derived Rab9 bearing transport vesicle at TGN elucidating
            role of Nef during viral pathogenesis.
REFERENCE   3  (bases 1 to 6909)
  AUTHORS   Cheung,P.Y., Limouse,C., Mabuchi,H. and Pfeffer,S.R.
  TITLE     Protein flexibility is required for vesicle tethering at the Golgi
  JOURNAL   Elife 4, e12790 (2015)
   PUBMED   26653856
  REMARK    GeneRIF: These unexpected features support a model in which GCC185
            collapses onto the Golgi surface, perhaps by binding to Rab
            GTPases, to mediate vesicle tethering.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6909)
  AUTHORS   Lin,Y.C., Chiang,T.C., Liu,Y.T., Tsai,Y.T., Jang,L.T. and Lee,F.J.
  TITLE     ARL4A acts with GCC185 to modulate Golgi complex organization
  JOURNAL   J Cell Sci 124 (Pt 23), 4014-4026 (2011)
   PUBMED   22159419
  REMARK    GeneRIF: Deletion of the ARL4A-interacting region of GCC185 results
            in inability to maintain Golgi structure and modulate
            endosome-to-Golgi transport.
REFERENCE   5  (bases 1 to 6909)
  AUTHORS   Hayes,G.L., Brown,F.C., Haas,A.K., Nottingham,R.M., Barr,F.A. and
            Pfeffer,S.R.
  TITLE     Multiple Rab GTPase binding sites in GCC185 suggest a model for
            vesicle tethering at the trans-Golgi
  JOURNAL   Mol Biol Cell 20 (1), 209-217 (2009)
   PUBMED   18946081
  REMARK    GeneRIF: Results show that GCC185 contains at least six binding
            sites for as many as 14 different Rab GTPases across its entire
            length.
REFERENCE   6  (bases 1 to 6909)
  AUTHORS   Burguete,A.S., Fenn,T.D., Brunger,A.T. and Pfeffer,S.R.
  TITLE     Rab and Arl GTPase family members cooperate in the localization of
            the golgin GCC185
  JOURNAL   Cell 132 (2), 286-298 (2008)
   PUBMED   18243103
  REMARK    GeneRIF: Rab and Arl GTPase family members cooperate in the
            localization of GCC2.
REFERENCE   7  (bases 1 to 6909)
  AUTHORS   Luke,M.R., Kjer-Nielsen,L., Brown,D.L., Stow,J.L. and Gleeson,P.A.
  TITLE     GRIP domain-mediated targeting of two new coiled-coil proteins,
            GCC88 and GCC185, to subcompartments of the trans-Golgi network
  JOURNAL   J Biol Chem 278 (6), 4216-4226 (2003)
   PUBMED   12446665
  REMARK    GeneRIF: targeting to subcompartments of trans-Golgi network by
            GRIP domain
REFERENCE   8  (bases 1 to 6909)
  AUTHORS   Krackhardt,A.M., Witzens,M., Harig,S., Hodi,F.S., Zauls,A.J.,
            Chessia,M., Barrett,P. and Gribben,J.G.
  TITLE     Identification of tumor-associated antigens in chronic lymphocytic
            leukemia by SEREX
  JOURNAL   Blood 100 (6), 2123-2131 (2002)
   PUBMED   12200376
REFERENCE   9  (bases 1 to 6909)
  AUTHORS   Eichmuller,S., Usener,D., Dummer,R., Stein,A., Thiel,D. and
            Schadendorf,D.
  TITLE     Serological detection of cutaneous T-cell lymphoma-associated
            antigens
  JOURNAL   Proc Natl Acad Sci U S A 98 (2), 629-634 (2001)
   PUBMED   11149944
REFERENCE   10 (bases 1 to 6909)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068941.7 and AC012487.12.
            
            On Jun 2, 2019 this sequence version replaced NM_181453.3.
            
            Summary: The protein encoded by this gene is a peripheral membrane
            protein localized to the trans-Golgi network. It is sensitive to
            brefeldin A. This encoded protein contains a GRIP domain which is
            thought to be used in targeting. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jul 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            CCDS Note: This CCDS representation does not have any full-length
            human transcript support, but it is supported by overlapping
            partial transcript evidence, including AF432211.1, AK090813.1,
            AK308707.1, and several tiled ESTs. This representation is also
            supported by studies in PMID:18946081, which functionally
            characterizes this protein, and by homology support in mouse
            (NP_081651.2, CCDS23859.1) and rat (NP_001101103.1). PMID:18243103
            also describes a partial crystal structure for this protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.24154.1,
                                           SRR1803614.9197.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309863.11/ ENSP00000307939.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                AC068941.7         89008-89082
            76-132              AC068941.7         89435-89491
            133-217             AC068941.7         90830-90914
            218-285             AC068941.7         92201-92268
            286-390             AC012487.12        8350-8454
            391-2856            AC012487.12        9021-11486
            2857-2929           AC012487.12        12197-12269
            2930-3030           AC012487.12        14905-15005
            3031-3129           AC012487.12        15122-15220
            3130-3249           AC012487.12        21067-21186
            3250-3414           AC012487.12        21657-21821
            3415-3519           AC012487.12        22432-22536
            3520-3682           AC012487.12        23519-23681
            3683-3783           AC012487.12        25006-25106
            3784-3861           AC012487.12        25201-25278
            3862-3999           AC012487.12        25881-26018
            4000-4121           AC012487.12        27069-27190
            4122-4298           AC012487.12        29208-29384
            4299-4516           AC012487.12        31943-32160
            4517-4711           AC012487.12        34661-34855
            4712-4851           AC012487.12        36340-36479
            4852-5053           AC012487.12        38923-39124
            5054-6909           AC012487.12        46930-48785
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.3"
     gene            1..6909
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="GRIP and coiled-coil domain containing 2"
                     /db_xref="GeneID:9648"
                     /db_xref="HGNC:HGNC:23218"
                     /db_xref="MIM:612711"
     exon            1..75
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..5124
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="isoform 1 is encoded by transcript variant 1; CTCL
                     tumor antigen se1-1; CLL-associated antigen KW-11; GCC
                     protein, 185-kD; GRIP and coiled-coil domain-containing
                     protein 2; Golgi coiled-coil protein GCC185; Ran-binding
                     protein 2-like 4; renal carcinoma antigen NY-REN-53; 185
                     kDa Golgi coiled-coil protein"
                     /codon_start=1
                     /product="GRIP and coiled-coil domain-containing protein 2
                     isoform 1"
                     /protein_id="NP_852118.2"
                     /db_xref="CCDS:CCDS33268.1"
                     /db_xref="GeneID:9648"
                     /db_xref="HGNC:HGNC:23218"
                     /db_xref="MIM:612711"
                     /translation="MEDLVQDGVASPATPGTGKSKLETLPKEDLIKFAKKQMMLIQKA
                     KSRCTELEKEIEELRSKPVTEGTGDIIKALTERLDALLLEKAETEQQCLSLKKENIKM
                     KQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAM
                     NTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQ
                     LQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNEL
                     KENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKV
                     KHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLL
                     AKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEK
                     EKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESM
                     KQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQE
                     KNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLI
                     LELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEV
                     LSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDN
                     LNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKC
                     NLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEA
                     LQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLE
                     QKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLEKECKEKEEKINKIKL
                     VAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQGAESYKNLLLEYEKQS
                     EQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLARIETLQSNAKLLEVQ
                     ILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQLQKQKKQLQKTMQELE
                     LVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQEIKIQKQKQETLQEEI
                     TSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQASLKGELEASQQQVEV
                     YKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEECRAAKAEQATVTSEF
                     ESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKIKLQDSQNNLQINVSE
                     LQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMMKSEHTQTVSQLTSQN
                     EVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEPTTRSPVSSQQSLKNL
                     RERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLEQLLNSPETKLEPPLW
                     HAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQERE
                     KSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLAAVAQGEEENAS
                     RSSGWASYLHSWSGLR"
     exon            76..132
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            133..217
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            218..285
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            286..390
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            391..2856
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..2929
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            2930..3030
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3031..3129
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3249
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3250..3414
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3519
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3520..3682
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3783
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3861
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..3999
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4000..4121
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4122..4298
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4299..4516
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4517..4711
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4712..4851
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4852..5053
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            5054..6909
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5154..5159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAGA"
     polyA_site      5174
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="major polyA site"
     regulatory      5365..5370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAAA"
     polyA_site      5385
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="major polyA site"
     regulatory      5827..5832
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATACA"
     regulatory      5852..5857
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAAA"
     polyA_site      5853
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
     polyA_site      5877
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="major polyA site"
     regulatory      6060..6065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAAA"
     polyA_site      6083
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
     regulatory      6787..6792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: GATAAA"
     polyA_site      6812
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
ORIGIN      
        1 agaggctggc gcaaacagaa gtgcagcggt ggcggcggct ggttgcgggc cggcggcggg
       61 ctggcggaga tggaggatct tgttcaagat ggggtggctt caccagctac ccctgggacc
      121 gggaaatcta agctggaaac attgcccaaa gaagacctca tcaagtttgc caagaaacag
      181 atgatgctaa tacagaaagc taaatcaagg tgtacagaat tggagaaaga aattgaagaa
      241 ctcagatcaa aacctgttac tgaaggaact ggtgatatta ttaaggcatt aactgaacgt
      301 ctggatgctc ttcttctgga aaaagcagag actgagcaac agtgtctttc tctgaaaaag
      361 gaaaatataa aaatgaagca agaggttgag gattctgtaa caaagatggg agatgcacat
      421 aaggagttgg aacaatcaca tataaactat gtgaaagaaa ttgaaaattt gaaaaatgag
      481 ttgatggcag tacgttccaa atacagtgaa gacaaagcta acttacaaaa gcagctggaa
      541 gaagcaatga atacgcaatt agaactttca gaacaactta aatttcagaa caactctgaa
      601 gataatgtta aaaaactaca agaagagatt gagaaaatta ggccaggctt tgaggagcaa
      661 attttatatc tgcaaaagca attagacgct accactgatg aaaagaagga aacagttact
      721 caactccaaa atatcattga ggctaattct cagcattacc aaaaaaatat taatagtttg
      781 caggaagagc ttttacagtt gaaagctata caccaagaag aggtgaaaga gttgatgtgc
      841 cagattgaag catcagctaa ggaacatgaa gcagagataa ataagttgaa cgagctaaaa
      901 gagaacttag taaaacaatg tgaggcaagt gaaaagaaca tccagaagaa atatgaatgt
      961 gagttagaaa atttaaggaa agccacctca aatgcaaacc aagacaatca gatatgttct
     1021 attctcttgc aagaaaatac atttgtagaa caagtagtaa atgaaaaagt caaacactta
     1081 gaagatacct taaaagaact tgaatctcaa cacagtatct taaaagatga ggtaacttat
     1141 atgaataatc ttaagttaaa acttgaaatg gatgctcaac atataaagga tgagtttttt
     1201 catgaacggg aagacttaga gtttaaaatt aatgaattat tactagctaa agaagaacag
     1261 ggctgtgtaa ttgaaaaatt aaaatctgag ctagcaggtt taaataaaca gttttgctat
     1321 actgtagaac agcataacag agaagtacag agtcttaagg aacaacatca aaaagaaata
     1381 tcagaactaa atgagacatt tttgtcagat tcagaaaaag aaaaattaac attaatgttt
     1441 gaaatacagg gtcttaagga acagtgtgaa aacctacagc aagaaaagca agaagcaatt
     1501 ttaaattatg agagtttacg agagattatg gaaattttac aaacagaact gggggaatct
     1561 gctggaaaaa taagtcaaga gttcgaatca atgaagcaac agcaagcatc tgatgttcat
     1621 gaactgcagc agaagctcag aactgctttt actgaaaaag atgcccttct cgaaactgtg
     1681 aatcgcctcc agggagaaaa tgaaaagtta ctatctcaac aagaattggt accagaactt
     1741 gaaaatacca taaagaacct tcaagaaaag aatggagtat acttacttag tctcagtcaa
     1801 agagatacca tgttaaaaga attagaagga aagataaatt ctcttactga ggaaaaagat
     1861 gattttataa ataaactgaa aaattcccat gaagaaatgg ataatttcca taagaaatgt
     1921 gaaagggaag aaagattgat tcttgaactt gggaagaaag tagagcaaac aatccagtac
     1981 aacagtgaac tagaacaaaa ggtaaatgaa ttaacaggag gactagagga gactttaaaa
     2041 gaaaaggatc aaaatgacca aaaactagaa aaacttatgg ttcaaatgaa agttctctct
     2101 gaagacaaag aagtattgtc agctgaagtg aagtctcttt atgaggaaaa caataaactc
     2161 agttcagaaa aaaaacagtt gagtagggat ttggaggttt ttttgtctca aaaagaagat
     2221 gttatcctta aagaacatat tactcaatta gaaaagaaac ttcagttaat ggttgaagag
     2281 caagataatt taaataaact gcttgaaaat gagcaagttc agaagttatt tgttaaaact
     2341 cagttgtatg gttttcttaa agaaatggga tcagaagttt cagaagacag tgaagagaaa
     2401 gatgttgtta atgtcctaca ggcagtcggt gaatccttgg caaaaataaa tgaggaaaaa
     2461 tgcaacctgg cttttcagcg tgatgaaaaa gtattagagt tagaaaaaga gattaagtgc
     2521 cttcaagaag agagtgtagt tcagtgtgaa gaacttaagt ctttattgag agactatgag
     2581 caagagaaag ttctcttaag gaaagagtta gaagaaatac agtcagaaaa agaggccctg
     2641 cagtctgatc ttctagaaat gaagaatgct aatgaaaaaa caaggcttga aaatcagaat
     2701 cttttaattc aagttgaaga agtatctcaa acatgtagca aaagtgaaat ccataatgaa
     2761 aaagaaaaat gttttataaa ggaacatgaa aacctaaagc cactactaga acaaaaagaa
     2821 ttacgagata ggagagcaga gttgatacta ttaaaggatt ccttagcaaa atcaccttct
     2881 gtaaaaaatg atcctctgtc ttcagtaaaa gagttggaag aaaaaataga aaatctggaa
     2941 aaagaatgca aagaaaagga ggagaaaata aataagataa aattagttgc cgtaaaggca
     3001 aagaaagaac tagattccag cagaaaagag acccagactg tgaaggaaga acttgaatct
     3061 cttcgatcag aaaaggacca gttatctgct tccatgagag atctcattca aggagcagaa
     3121 agctataaga atcttttatt agaatatgaa aagcagtcag agcaactgga tgtggaaaaa
     3181 gaacgtgcta ataattttga gcatcgtatt gaagacctta caagacaatt aagaaattcg
     3241 actttgcagt gtgaaacaat aaattctgat aatgaagatc tcctggctcg tattgagaca
     3301 ttacagtcta atgccaaatt attagaagta cagattttag aagtccagag agccaaagca
     3361 atggtagaca aagaattaga agctgaaaaa cttcagaaag aacagaagat aaaggaacat
     3421 gccactactg taaatgaact tgaagaactt caggtacaac ttcaaaagca aaagaaacag
     3481 cttcagaaaa ccatgcaaga attagagctg gttaaaaagg atgcccaaca aaccacattg
     3541 atgaatatgg aaatagctga ttatgaacgt ttgatgaaag aactaaatca aaagttaact
     3601 aataaaaaca acaagataga agatttggag caagaaataa aaattcaaaa acagaaacaa
     3661 gaaaccctac aagaagaaat aacttcatta cagtcttcag tacaacaata tgaagaaaaa
     3721 aacaccaaaa tcaagcaatt gcttgtgaaa accaaaaagg aactggcaga ttcaaagcaa
     3781 gcagaaactg atcacttaat acttcaagca tctttaaaag gtgagctgga ggcaagccag
     3841 cagcaagtag aagtctataa aatacagctg gctgaaataa catcagagaa gcacaaaatc
     3901 cacgagcacc tgaaaacctc tgcggaacag caccagcgta cgctaagtgc ataccagcag
     3961 agagtgacag cactacagga agagtgccgt gctgccaagg cagaacaagc tactgtaacc
     4021 tctgaattcg agagctacaa agtccgagtt cataatgttc taaaacaaca gaaaaataaa
     4081 tctatgtctc aggctgaaac tgagggcgct aaacaagaaa gggaacatct ggaaatgctg
     4141 attgaccagc taaaaatcaa attacaagat agccaaaata acttacagat taatgtatct
     4201 gaacttcaaa cattgcagtc tgaacatgat acactgctag aaaggcacaa caagatgctg
     4261 caggaaactg tgtccaaaga ggcggaactc cgggaaaaat tgtgttcaat acagtcagag
     4321 aacatgatga tgaaatctga acatacacag actgtgagtc agctaacatc ccagaacgag
     4381 gtccttcgaa atagcttccg agatcaagtg cgacatttgc aggaagaaca cagaaagaca
     4441 gtggagacat tacagcagca gctctccaag atggaagcac agctcttcca gcttaagaat
     4501 gaaccgacca caagaagccc agtttcctct caacaatctt tgaagaacct tcgagaaagg
     4561 agaaacacag acctcccgct tctagacatg cacactgtaa cccgggaaga gggagaaggc
     4621 atggagacaa ctgatacgga gtctgtgtct tccgccagca catacacaca gtctttagag
     4681 cagctgctta actctcccga aactaaactt gagcctccat tatggcatgc tgaatttacc
     4741 aaagaagaat tggttcagaa gctcagttcc accacaaaaa gtgcagatca cttaaacggc
     4801 ctgcttcggg aaacagaagc aaccaatgca attcttatgg agcaaattaa gcttctcaaa
     4861 agtgaaataa gaagattgga aaggaatcaa gagcgagaga agtctgcagc taacctggaa
     4921 tacttgaaga acgtcttgct gcagttcatt ttcttgaaac caggtagtga aagagagaga
     4981 cttcttcctg ttataaatac gatgttgcag ctcagccctg aagaaaaggg aaaacttgct
     5041 gcggttgctc aaggtgagga agaaaatgct tcccgttctt ctggatgggc atcctatctt
     5101 catagttggt ctggacttcg ataggttgat ggaaggaata tttttattaa ccaaatagaa
     5161 tctatttaca aaaatggttc acgtatatta ccacaattct tttgtcaaaa agtgtgtata
     5221 tatgtttgca tctacatata tttgtacatc tatatgacag atgtatttta aaagtttcat
     5281 cttgaagtaa aagtacaaca gcttgaagtg ttgatagcag gccacagccc tctaactcat
     5341 gtgatttccc atgcatgctg ccagaataaa accaccagga atgaattcac tccccacttc
     5401 tctggaacct caggacccgc ccatttctcg gcagtactgt gaattttgaa gttaaactaa
     5461 attttggtac cataccaact ggaatttagg ctttaaaaat aatgtttcaa ggccaggtgt
     5521 ggtgattcat gcctgaaatc ccactacttt gggaggctga ggctggagaa ttgcttgagg
     5581 ctagtgagct gtgactccca ctgcactcca gctcggggaa cagagcgaga ccttgtctct
     5641 aaaaataata gtaataaaat aaaaataacg ttttatgact atttattgca aggtcagagt
     5701 tacagattgt tataaattgt tgagaaattt ttgtgattag aatatgaagg aaaaagcttt
     5761 gttggtaaaa gtgacatgtt aaggggctat gaagtaaata tgctgcagtt aattatgcta
     5821 agttaaaata cagtttagtt atttgcttta aaataaactc ttcttttttt ctttaaagta
     5881 tactatctca aaactcatta tgttgtcaga gccctagagc tggctagtgt aacactgact
     5941 atgagtaggt gggcccacac ttgagttgag gtgatttcat ggtgtctttc caggctcttg
     6001 atagggtgtc actgcatgca agccatgaat ctgttttgag aatcctctcc attttcccaa
     6061 ataaaaacct atcacaacag tgactatatc actcagcatt ggatctaaat ataaaagtgg
     6121 tgctttcagt gtttttggca gatagtgttc cataagcttt ccatcagaag ggattttaga
     6181 caccttagag gtccgtgcta catcgtcaca gttcctccga ataaccttag gtggtagtgt
     6241 tacttgcctt tgacacctct gcatatgttt taatgactag atccaaactg tgttgttctt
     6301 aaatcaaaaa ttggataatt tgtaatattt atgtgttaat cacacagtat gctctctgaa
     6361 gttctcttaa gccttcagtt tatactctta atttaatttt ctttctgagc tggagaactg
     6421 gctttgcact ttggttacac agaacattgg tttccaattc agtttaactg aaatttgctg
     6481 ctgatatgtt gagtttgttc tttaaaaaat agctcatata tctcatcttt cctcctgtct
     6541 tagaagaaca gacctaacta gtgaatgtat taatgaaaat gcatctattt cagagctgac
     6601 atgaagagtt tagttttttt actttataaa ctgtgaatat gagtatgcca gctgcatacg
     6661 atgtaactaa tcatatttaa atatatttca ctttctcttt gactttagac cttttgaagt
     6721 ctgtataaac ttgttttgaa atatagtctc tgcttacgaa tgtcataaca aaataatttt
     6781 ttgcatgata aaaaattact ttgattacaa aaggcatatt ctttcatggt ttctgcaatg
     6841 agaggaagtg taatgattat tttaatattt ctattaaata tgtttaactg tatattttta
     6901 tggctgctc
//
