LOCUS       NM_001410194            6824 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2),
            transcript variant 2, mRNA.
ACCESSION   NM_001410194 NR_028063
VERSION     NM_001410194.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Liang,X.H., Sun,H., Hsu,C.W., Nichols,J.G., Vickers,T.A., De
            Hoyos,C.L. and Crooke,S.T.
  TITLE     Golgi-endosome transport mediated by M6PR facilitates release of
            antisense oligonucleotides from endosomes
  JOURNAL   Nucleic Acids Res 48 (3), 1372-1391 (2020)
   PUBMED   31840180
  REMARK    GeneRIF: The co-localization of M6PR and of GCC2 with ASOs is
            influenced by the PS modifications, which have been shown to
            enhance the affinity of ASOs for proteins, suggesting that
            localization of these proteins to LEs is mediated by ASO-protein
            interactions. Reduction of M6PR levels also decreased PS-ASO
            activity in mouse cells and in livers of mice treated
            subcutaneously with PS-ASO, indicating a conserved mechanism.
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Kumar,M., Kaur,S., Nazir,A. and Tripathi,R.K.
  TITLE     HIV-1 Nef binds with human GCC185 protein and regulates mannose 6
            phosphate receptor recycling
  JOURNAL   Biochem Biophys Res Commun 474 (1), 137-145 (2016)
   PUBMED   27105913
  REMARK    GeneRIF: In light of existing report suggesting critical role of
            Nef-GCC185 interaction reveals valuable mechanistic insights
            affecting specific protein transport pathway in docking of late
            endosome derived Rab9 bearing transport vesicle at TGN elucidating
            role of Nef during viral pathogenesis.
REFERENCE   3  (bases 1 to 6824)
  AUTHORS   Cheung,P.Y., Limouse,C., Mabuchi,H. and Pfeffer,S.R.
  TITLE     Protein flexibility is required for vesicle tethering at the Golgi
  JOURNAL   Elife 4, e12790 (2015)
   PUBMED   26653856
  REMARK    GeneRIF: These unexpected features support a model in which GCC185
            collapses onto the Golgi surface, perhaps by binding to Rab
            GTPases, to mediate vesicle tethering.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6824)
  AUTHORS   Lin,Y.C., Chiang,T.C., Liu,Y.T., Tsai,Y.T., Jang,L.T. and Lee,F.J.
  TITLE     ARL4A acts with GCC185 to modulate Golgi complex organization
  JOURNAL   J Cell Sci 124 (Pt 23), 4014-4026 (2011)
   PUBMED   22159419
  REMARK    GeneRIF: Deletion of the ARL4A-interacting region of GCC185 results
            in inability to maintain Golgi structure and modulate
            endosome-to-Golgi transport.
REFERENCE   5  (bases 1 to 6824)
  AUTHORS   Hayes,G.L., Brown,F.C., Haas,A.K., Nottingham,R.M., Barr,F.A. and
            Pfeffer,S.R.
  TITLE     Multiple Rab GTPase binding sites in GCC185 suggest a model for
            vesicle tethering at the trans-Golgi
  JOURNAL   Mol Biol Cell 20 (1), 209-217 (2009)
   PUBMED   18946081
  REMARK    GeneRIF: Results show that GCC185 contains at least six binding
            sites for as many as 14 different Rab GTPases across its entire
            length.
REFERENCE   6  (bases 1 to 6824)
  AUTHORS   Burguete,A.S., Fenn,T.D., Brunger,A.T. and Pfeffer,S.R.
  TITLE     Rab and Arl GTPase family members cooperate in the localization of
            the golgin GCC185
  JOURNAL   Cell 132 (2), 286-298 (2008)
   PUBMED   18243103
  REMARK    GeneRIF: Rab and Arl GTPase family members cooperate in the
            localization of GCC2.
REFERENCE   7  (bases 1 to 6824)
  AUTHORS   Luke,M.R., Kjer-Nielsen,L., Brown,D.L., Stow,J.L. and Gleeson,P.A.
  TITLE     GRIP domain-mediated targeting of two new coiled-coil proteins,
            GCC88 and GCC185, to subcompartments of the trans-Golgi network
  JOURNAL   J Biol Chem 278 (6), 4216-4226 (2003)
   PUBMED   12446665
  REMARK    GeneRIF: targeting to subcompartments of trans-Golgi network by
            GRIP domain
REFERENCE   8  (bases 1 to 6824)
  AUTHORS   Krackhardt,A.M., Witzens,M., Harig,S., Hodi,F.S., Zauls,A.J.,
            Chessia,M., Barrett,P. and Gribben,J.G.
  TITLE     Identification of tumor-associated antigens in chronic lymphocytic
            leukemia by SEREX
  JOURNAL   Blood 100 (6), 2123-2131 (2002)
   PUBMED   12200376
REFERENCE   9  (bases 1 to 6824)
  AUTHORS   Eichmuller,S., Usener,D., Dummer,R., Stein,A., Thiel,D. and
            Schadendorf,D.
  TITLE     Serological detection of cutaneous T-cell lymphoma-associated
            antigens
  JOURNAL   Proc Natl Acad Sci U S A 98 (2), 629-634 (2001)
   PUBMED   11149944
REFERENCE   10 (bases 1 to 6824)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068941.7 and AC012487.12.
            
            On Jul 29, 2022 this sequence version replaced NR_028063.2.
            
            Summary: The protein encoded by this gene is a peripheral membrane
            protein localized to the trans-Golgi network. It is sensitive to
            brefeldin A. This encoded protein contains a GRIP domain which is
            thought to be used in targeting. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jul 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC146789.1, AB002334.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                AC068941.7         89008-89082
            76-132              AC068941.7         89435-89491
            133-200             AC068941.7         92201-92268
            201-305             AC012487.12        8350-8454
            306-2771            AC012487.12        9021-11486
            2772-2844           AC012487.12        12197-12269
            2845-2945           AC012487.12        14905-15005
            2946-3044           AC012487.12        15122-15220
            3045-3164           AC012487.12        21067-21186
            3165-3329           AC012487.12        21657-21821
            3330-3434           AC012487.12        22432-22536
            3435-3597           AC012487.12        23519-23681
            3598-3698           AC012487.12        25006-25106
            3699-3776           AC012487.12        25201-25278
            3777-3914           AC012487.12        25881-26018
            3915-4036           AC012487.12        27069-27190
            4037-4213           AC012487.12        29208-29384
            4214-4431           AC012487.12        31943-32160
            4432-4626           AC012487.12        34661-34855
            4627-4766           AC012487.12        36340-36479
            4767-4968           AC012487.12        38923-39124
            4969-6824           AC012487.12        46930-48785
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.3"
     gene            1..6824
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="GRIP and coiled-coil domain containing 2"
                     /db_xref="GeneID:9648"
                     /db_xref="HGNC:HGNC:23218"
                     /db_xref="MIM:612711"
     exon            1..75
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            76..132
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            133..200
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            201..305
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..5039
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="isoform 2 is encoded by transcript variant 2; CTCL
                     tumor antigen se1-1; CLL-associated antigen KW-11; GCC
                     protein, 185-kD; GRIP and coiled-coil domain-containing
                     protein 2; Golgi coiled-coil protein GCC185; Ran-binding
                     protein 2-like 4; renal carcinoma antigen NY-REN-53; 185
                     kDa Golgi coiled-coil protein"
                     /codon_start=1
                     /product="GRIP and coiled-coil domain-containing protein 2
                     isoform 2"
                     /protein_id="NP_001397123.1"
                     /db_xref="GeneID:9648"
                     /db_xref="HGNC:HGNC:23218"
                     /db_xref="MIM:612711"
                     /translation="MKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKY
                     SEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQK
                     QLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEA
                     SAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSIL
                     LQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFF
                     HEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQK
                     EISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTE
                     LGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQ
                     ELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEE
                     MDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLE
                     KLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHIT
                     QLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVL
                     QAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKV
                     LLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKE
                     KCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLE
                     KECKEKEEKINKIKLVAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQG
                     AESYKNLLLEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLA
                     RIETLQSNAKLLEVQILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQL
                     QKQKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQE
                     IKIQKQKQETLQEEITSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQA
                     SLKGELEASQQQVEVYKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEE
                     CRAAKAEQATVTSEFESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKI
                     KLQDSQNNLQINVSELQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMM
                     KSEHTQTVSQLTSQNEVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEP
                     TTRSPVSSQQSLKNLRERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLE
                     QLLNSPETKLEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKL
                     LKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEK
                     GKLAAVAQGEEENASRSSGWASYLHSWSGLR"
     exon            306..2771
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            2772..2844
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            2845..2945
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..3044
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3164
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3329
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3330..3434
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3597
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3598..3698
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3699..3776
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..3914
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            3915..4036
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4037..4213
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4214..4431
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4432..4626
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4627..4766
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4767..4968
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     exon            4969..6824
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5069..5074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAGA"
     polyA_site      5089
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="major polyA site"
     regulatory      5280..5285
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAAA"
     polyA_site      5300
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="major polyA site"
     regulatory      5742..5747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATACA"
     regulatory      5767..5772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAAA"
     polyA_site      5768
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
     polyA_site      5792
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="major polyA site"
     regulatory      5975..5980
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: AATAAA"
     polyA_site      5998
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
     regulatory      6702..6707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
                     /note="hexamer: GATAAA"
     polyA_site      6727
                     /gene="GCC2"
                     /gene_synonym="GCC185; RANBP2L4; REN53"
ORIGIN      
        1 agaggctggc gcaaacagaa gtgcagcggt ggcggcggct ggttgcgggc cggcggcggg
       61 ctggcggaga tggaggatct tgttcaagat ggggtggctt caccagctac ccctgggacc
      121 gggaaatcta agaattggag aaagaaattg aagaactcag atcaaaacct gttactgaag
      181 gaactggtga tattattaag gcattaactg aacgtctgga tgctcttctt ctggaaaaag
      241 cagagactga gcaacagtgt ctttctctga aaaaggaaaa tataaaaatg aagcaagagg
      301 ttgaggattc tgtaacaaag atgggagatg cacataagga gttggaacaa tcacatataa
      361 actatgtgaa agaaattgaa aatttgaaaa atgagttgat ggcagtacgt tccaaataca
      421 gtgaagacaa agctaactta caaaagcagc tggaagaagc aatgaatacg caattagaac
      481 tttcagaaca acttaaattt cagaacaact ctgaagataa tgttaaaaaa ctacaagaag
      541 agattgagaa aattaggcca ggctttgagg agcaaatttt atatctgcaa aagcaattag
      601 acgctaccac tgatgaaaag aaggaaacag ttactcaact ccaaaatatc attgaggcta
      661 attctcagca ttaccaaaaa aatattaata gtttgcagga agagctttta cagttgaaag
      721 ctatacacca agaagaggtg aaagagttga tgtgccagat tgaagcatca gctaaggaac
      781 atgaagcaga gataaataag ttgaacgagc taaaagagaa cttagtaaaa caatgtgagg
      841 caagtgaaaa gaacatccag aagaaatatg aatgtgagtt agaaaattta aggaaagcca
      901 cctcaaatgc aaaccaagac aatcagatat gttctattct cttgcaagaa aatacatttg
      961 tagaacaagt agtaaatgaa aaagtcaaac acttagaaga taccttaaaa gaacttgaat
     1021 ctcaacacag tatcttaaaa gatgaggtaa cttatatgaa taatcttaag ttaaaacttg
     1081 aaatggatgc tcaacatata aaggatgagt tttttcatga acgggaagac ttagagttta
     1141 aaattaatga attattacta gctaaagaag aacagggctg tgtaattgaa aaattaaaat
     1201 ctgagctagc aggtttaaat aaacagtttt gctatactgt agaacagcat aacagagaag
     1261 tacagagtct taaggaacaa catcaaaaag aaatatcaga actaaatgag acatttttgt
     1321 cagattcaga aaaagaaaaa ttaacattaa tgtttgaaat acagggtctt aaggaacagt
     1381 gtgaaaacct acagcaagaa aagcaagaag caattttaaa ttatgagagt ttacgagaga
     1441 ttatggaaat tttacaaaca gaactggggg aatctgctgg aaaaataagt caagagttcg
     1501 aatcaatgaa gcaacagcaa gcatctgatg ttcatgaact gcagcagaag ctcagaactg
     1561 cttttactga aaaagatgcc cttctcgaaa ctgtgaatcg cctccaggga gaaaatgaaa
     1621 agttactatc tcaacaagaa ttggtaccag aacttgaaaa taccataaag aaccttcaag
     1681 aaaagaatgg agtatactta cttagtctca gtcaaagaga taccatgtta aaagaattag
     1741 aaggaaagat aaattctctt actgaggaaa aagatgattt tataaataaa ctgaaaaatt
     1801 cccatgaaga aatggataat ttccataaga aatgtgaaag ggaagaaaga ttgattcttg
     1861 aacttgggaa gaaagtagag caaacaatcc agtacaacag tgaactagaa caaaaggtaa
     1921 atgaattaac aggaggacta gaggagactt taaaagaaaa ggatcaaaat gaccaaaaac
     1981 tagaaaaact tatggttcaa atgaaagttc tctctgaaga caaagaagta ttgtcagctg
     2041 aagtgaagtc tctttatgag gaaaacaata aactcagttc agaaaaaaaa cagttgagta
     2101 gggatttgga ggtttttttg tctcaaaaag aagatgttat ccttaaagaa catattactc
     2161 aattagaaaa gaaacttcag ttaatggttg aagagcaaga taatttaaat aaactgcttg
     2221 aaaatgagca agttcagaag ttatttgtta aaactcagtt gtatggtttt cttaaagaaa
     2281 tgggatcaga agtttcagaa gacagtgaag agaaagatgt tgttaatgtc ctacaggcag
     2341 tcggtgaatc cttggcaaaa ataaatgagg aaaaatgcaa cctggctttt cagcgtgatg
     2401 aaaaagtatt agagttagaa aaagagatta agtgccttca agaagagagt gtagttcagt
     2461 gtgaagaact taagtcttta ttgagagact atgagcaaga gaaagttctc ttaaggaaag
     2521 agttagaaga aatacagtca gaaaaagagg ccctgcagtc tgatcttcta gaaatgaaga
     2581 atgctaatga aaaaacaagg cttgaaaatc agaatctttt aattcaagtt gaagaagtat
     2641 ctcaaacatg tagcaaaagt gaaatccata atgaaaaaga aaaatgtttt ataaaggaac
     2701 atgaaaacct aaagccacta ctagaacaaa aagaattacg agataggaga gcagagttga
     2761 tactattaaa ggattcctta gcaaaatcac cttctgtaaa aaatgatcct ctgtcttcag
     2821 taaaagagtt ggaagaaaaa atagaaaatc tggaaaaaga atgcaaagaa aaggaggaga
     2881 aaataaataa gataaaatta gttgccgtaa aggcaaagaa agaactagat tccagcagaa
     2941 aagagaccca gactgtgaag gaagaacttg aatctcttcg atcagaaaag gaccagttat
     3001 ctgcttccat gagagatctc attcaaggag cagaaagcta taagaatctt ttattagaat
     3061 atgaaaagca gtcagagcaa ctggatgtgg aaaaagaacg tgctaataat tttgagcatc
     3121 gtattgaaga ccttacaaga caattaagaa attcgacttt gcagtgtgaa acaataaatt
     3181 ctgataatga agatctcctg gctcgtattg agacattaca gtctaatgcc aaattattag
     3241 aagtacagat tttagaagtc cagagagcca aagcaatggt agacaaagaa ttagaagctg
     3301 aaaaacttca gaaagaacag aagataaagg aacatgccac tactgtaaat gaacttgaag
     3361 aacttcaggt acaacttcaa aagcaaaaga aacagcttca gaaaaccatg caagaattag
     3421 agctggttaa aaaggatgcc caacaaacca cattgatgaa tatggaaata gctgattatg
     3481 aacgtttgat gaaagaacta aatcaaaagt taactaataa aaacaacaag atagaagatt
     3541 tggagcaaga aataaaaatt caaaaacaga aacaagaaac cctacaagaa gaaataactt
     3601 cattacagtc ttcagtacaa caatatgaag aaaaaaacac caaaatcaag caattgcttg
     3661 tgaaaaccaa aaaggaactg gcagattcaa agcaagcaga aactgatcac ttaatacttc
     3721 aagcatcttt aaaaggtgag ctggaggcaa gccagcagca agtagaagtc tataaaatac
     3781 agctggctga aataacatca gagaagcaca aaatccacga gcacctgaaa acctctgcgg
     3841 aacagcacca gcgtacgcta agtgcatacc agcagagagt gacagcacta caggaagagt
     3901 gccgtgctgc caaggcagaa caagctactg taacctctga attcgagagc tacaaagtcc
     3961 gagttcataa tgttctaaaa caacagaaaa ataaatctat gtctcaggct gaaactgagg
     4021 gcgctaaaca agaaagggaa catctggaaa tgctgattga ccagctaaaa atcaaattac
     4081 aagatagcca aaataactta cagattaatg tatctgaact tcaaacattg cagtctgaac
     4141 atgatacact gctagaaagg cacaacaaga tgctgcagga aactgtgtcc aaagaggcgg
     4201 aactccggga aaaattgtgt tcaatacagt cagagaacat gatgatgaaa tctgaacata
     4261 cacagactgt gagtcagcta acatcccaga acgaggtcct tcgaaatagc ttccgagatc
     4321 aagtgcgaca tttgcaggaa gaacacagaa agacagtgga gacattacag cagcagctct
     4381 ccaagatgga agcacagctc ttccagctta agaatgaacc gaccacaaga agcccagttt
     4441 cctctcaaca atctttgaag aaccttcgag aaaggagaaa cacagacctc ccgcttctag
     4501 acatgcacac tgtaacccgg gaagagggag aaggcatgga gacaactgat acggagtctg
     4561 tgtcttccgc cagcacatac acacagtctt tagagcagct gcttaactct cccgaaacta
     4621 aacttgagcc tccattatgg catgctgaat ttaccaaaga agaattggtt cagaagctca
     4681 gttccaccac aaaaagtgca gatcacttaa acggcctgct tcgggaaaca gaagcaacca
     4741 atgcaattct tatggagcaa attaagcttc tcaaaagtga aataagaaga ttggaaagga
     4801 atcaagagcg agagaagtct gcagctaacc tggaatactt gaagaacgtc ttgctgcagt
     4861 tcattttctt gaaaccaggt agtgaaagag agagacttct tcctgttata aatacgatgt
     4921 tgcagctcag ccctgaagaa aagggaaaac ttgctgcggt tgctcaaggt gaggaagaaa
     4981 atgcttcccg ttcttctgga tgggcatcct atcttcatag ttggtctgga cttcgatagg
     5041 ttgatggaag gaatattttt attaaccaaa tagaatctat ttacaaaaat ggttcacgta
     5101 tattaccaca attcttttgt caaaaagtgt gtatatatgt ttgcatctac atatatttgt
     5161 acatctatat gacagatgta ttttaaaagt ttcatcttga agtaaaagta caacagcttg
     5221 aagtgttgat agcaggccac agccctctaa ctcatgtgat ttcccatgca tgctgccaga
     5281 ataaaaccac caggaatgaa ttcactcccc acttctctgg aacctcagga cccgcccatt
     5341 tctcggcagt actgtgaatt ttgaagttaa actaaatttt ggtaccatac caactggaat
     5401 ttaggcttta aaaataatgt ttcaaggcca ggtgtggtga ttcatgcctg aaatcccact
     5461 actttgggag gctgaggctg gagaattgct tgaggctagt gagctgtgac tcccactgca
     5521 ctccagctcg gggaacagag cgagaccttg tctctaaaaa taatagtaat aaaataaaaa
     5581 taacgtttta tgactattta ttgcaaggtc agagttacag attgttataa attgttgaga
     5641 aatttttgtg attagaatat gaaggaaaaa gctttgttgg taaaagtgac atgttaaggg
     5701 gctatgaagt aaatatgctg cagttaatta tgctaagtta aaatacagtt tagttatttg
     5761 ctttaaaata aactcttctt tttttcttta aagtatacta tctcaaaact cattatgttg
     5821 tcagagccct agagctggct agtgtaacac tgactatgag taggtgggcc cacacttgag
     5881 ttgaggtgat ttcatggtgt ctttccaggc tcttgatagg gtgtcactgc atgcaagcca
     5941 tgaatctgtt ttgagaatcc tctccatttt cccaaataaa aacctatcac aacagtgact
     6001 atatcactca gcattggatc taaatataaa agtggtgctt tcagtgtttt tggcagatag
     6061 tgttccataa gctttccatc agaagggatt ttagacacct tagaggtccg tgctacatcg
     6121 tcacagttcc tccgaataac cttaggtggt agtgttactt gcctttgaca cctctgcata
     6181 tgttttaatg actagatcca aactgtgttg ttcttaaatc aaaaattgga taatttgtaa
     6241 tatttatgtg ttaatcacac agtatgctct ctgaagttct cttaagcctt cagtttatac
     6301 tcttaattta attttctttc tgagctggag aactggcttt gcactttggt tacacagaac
     6361 attggtttcc aattcagttt aactgaaatt tgctgctgat atgttgagtt tgttctttaa
     6421 aaaatagctc atatatctca tctttcctcc tgtcttagaa gaacagacct aactagtgaa
     6481 tgtattaatg aaaatgcatc tatttcagag ctgacatgaa gagtttagtt tttttacttt
     6541 ataaactgtg aatatgagta tgccagctgc atacgatgta actaatcata tttaaatata
     6601 tttcactttc tctttgactt tagacctttt gaagtctgta taaacttgtt ttgaaatata
     6661 gtctctgctt acgaatgtca taacaaaata attttttgca tgataaaaaa ttactttgat
     6721 tacaaaaggc atattctttc atggtttctg caatgagagg aagtgtaatg attattttaa
     6781 tatttctatt aaatatgttt aactgtatat ttttatggct gctc
//
