LOCUS       NM_001485               2140 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens gastrulation brain homeobox 2 (GBX2), transcript
            variant 1, mRNA.
ACCESSION   NM_001485
VERSION     NM_001485.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Lin,Y.H., Zhang,B.Y. and Chen,Z.S.
  TITLE     circRERE regulates the expression of GBX2 through miR-1299 and
            ZC3H13/N6-methyladenosine (m6A) to promote growth and invasion of
            hepatocellular carcinoma cells
  JOURNAL   J Biosci 47 (2022)
   PUBMED   36222159
  REMARK    GeneRIF: circRERE regulates the expression of GBX2 through miR-1299
            and ZC3H13/N(6)-methyladenosine (m(6)A) to promote growth and
            invasion of hepatocellular carcinoma cells.
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Chen,X., Cheng,P. and Hu,C.
  TITLE     LncRNA FEZF1-AS1 accelerates the migration and invasion of
            laryngeal squamous cell carcinoma cells through miR-4497 targeting
            GBX2
  JOURNAL   Eur Arch Otorhinolaryngol 278 (5), 1523-1535 (2021)
   PUBMED   33550476
  REMARK    GeneRIF: LncRNA FEZF1-AS1 accelerates the migration and invasion of
            laryngeal squamous cell carcinoma cells through miR-4497 targeting
            GBX2.
REFERENCE   3  (bases 1 to 2140)
  AUTHORS   Li,X., Zhang,F., Ma,J., Ruan,X., Liu,X., Zheng,J., Liu,Y., Cao,S.,
            Shen,S., Shao,L., Cai,H., Li,Z. and Xue,Y.
  TITLE     NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification
            manner to facilitate the malignant biological behaviour of glioma
            cells
  JOURNAL   RNA Biol 18 (1), 47-63 (2021)
   PUBMED   32618493
  REMARK    GeneRIF: NCBP3/SNHG6 inhibits GBX2 transcription in a histone
            modification manner to facilitate the malignant biological
            behaviour of glioma cells.
REFERENCE   4  (bases 1 to 2140)
  AUTHORS   Wang,Y., Hui,J., Li,R., Fu,Q., Yang,P., Xiao,Y. and Hui,J.
  TITLE     GBX2, as a tumor promoter in lung adenocarcinoma, enhances cells
            viability, invasion and migration by regulating the AKT/ERK
            signaling pathway
  JOURNAL   J Gene Med 22 (2), e3147 (2020)
   PUBMED   31758726
  REMARK    GeneRIF: GBX2, as a tumor promoter in lung adenocarcinoma, enhances
            cells viability, invasion and migration by regulating the AKT/ERK
            signaling pathway.
REFERENCE   5  (bases 1 to 2140)
  AUTHORS   Jeyapala,R., Savio,A.J., Olkhov-Mitsel,E., Kamdar,S., Zhao,F.,
            Cuizon,C., Liu,R.S.C., Zlotta,A., Fleshner,N., van der Kwast,T. and
            Bapat,B.
  TITLE     GBX2 Methylation Is a Novel Prognostic Biomarker and Improves
            Prediction of Biochemical Recurrence Among Patients with Prostate
            Cancer Negative for Intraductal Carcinoma and Cribriform
            Architecture
  JOURNAL   Eur Urol Oncol 2 (3), 231-238 (2019)
   PUBMED   31200836
  REMARK    GeneRIF: Gastrulation brain homeobox 2 (GBX2) methylation is
            associated with Gleason score (GS), pathologic stage (pT), and
            biochemical recurrence (BCR) in prostate cancer (PCa). Among
            intraductal carcinoma/cribriform architecture (IDC/C)-negative
            patients, GBX2 methylation improves prediction of BCR.  Loss of
            TET2 in prostate cells results in greater GBX2 methylation.
REFERENCE   6  (bases 1 to 2140)
  AUTHORS   Gao,A.C., Lou,W. and Isaacs,J.T.
  TITLE     Enhanced GBX2 expression stimulates growth of human prostate cancer
            cells via transcriptional up-regulation of the interleukin 6 gene
  JOURNAL   Clin Cancer Res 6 (2), 493-497 (2000)
   PUBMED   10690529
REFERENCE   7  (bases 1 to 2140)
  AUTHORS   Gao,A.C., Lou,W. and Isaacs,J.T.
  TITLE     Down-regulation of homeobox gene GBX2 expression inhibits human
            prostate cancer clonogenic ability and tumorigenicity
  JOURNAL   Cancer Res 58 (7), 1391-1394 (1998)
   PUBMED   9537237
REFERENCE   8  (bases 1 to 2140)
  AUTHORS   Kowenz-Leutz,E., Herr,P., Niss,K. and Leutz,A.
  TITLE     The homeobox gene GBX2, a target of the myb oncogene, mediates
            autocrine growth and monocyte differentiation
  JOURNAL   Cell 91 (2), 185-195 (1997)
   PUBMED   9346236
REFERENCE   9  (bases 1 to 2140)
  AUTHORS   Lin,X., Swaroop,A., Vaccarino,F.M., Murtha,M.T., Haas,M., Ji,X.,
            Ruddle,F.H. and Leckman,J.F.
  TITLE     Characterization and sequence analysis of the human
            homeobox-containing gene GBX2
  JOURNAL   Genomics 31 (3), 335-342 (1996)
   PUBMED   8838315
  REMARK    GeneRIF: The accession number for the cDNA listed for the sequence
            data at the bottom of page 335 on the left side is incorrect. It
            should read U31468 instead of U31458.
REFERENCE   10 (bases 1 to 2140)
  AUTHORS   Matsui,T., Hirai,M., Hirano,M. and Kurosawa,Y.
  TITLE     The HOX complex neighbored by the EVX gene, as well as two other
            homeobox-containing genes, the GBX-class and the EN-class, are
            located on the same chromosomes 2 and 7 in humans
  JOURNAL   FEBS Lett 336 (1), 107-110 (1993)
   PUBMED   7903253
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC079135.6, BC137448.1, BF115559.1, AA176205.1 and BE463746.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001485.3.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC137449.1, AF118452.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306318.5/ ENSP00000302251.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               AC079135.6         35753-36129         c
            378-1646            BC137448.1         1-1269
            1647-1687           BF115559.1         26-66               c
            1688-1737           AA176205.1         436-485             c
            1738-1950           BE463746.1         165-377             c
            1951-2140           AA176205.1         33-222              c
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.2"
     gene            1..2140
                     /gene="GBX2"
                     /note="gastrulation brain homeobox 2"
                     /db_xref="GeneID:2637"
                     /db_xref="HGNC:HGNC:4186"
                     /db_xref="MIM:601135"
     exon            1..938
                     /gene="GBX2"
                     /inference="alignment:Splign:2.1.0"
     CDS             416..1462
                     /gene="GBX2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homeobox protein GBX-2; gastrulation and brain-specific
                     homeobox protein 2"
                     /codon_start=1
                     /product="homeobox protein GBX-2 isoform 1"
                     /protein_id="NP_001476.2"
                     /db_xref="CCDS:CCDS2515.1"
                     /db_xref="GeneID:2637"
                     /db_xref="HGNC:HGNC:4186"
                     /db_xref="MIM:601135"
                     /translation="MSAAFPPSLMMMQRPLGSSTAFSIDSLIGSPPQPSPGHFVYTGY
                     PMFMPYRPVVLPPPPPPPPALPQAALQPALPPAHPHHQIPSLPTGFCSSLAQGMALTS
                     TLMATLPGGFSASPQHQEAAAARKFAPQPLPGGGNFDKAEALQADAEDGKGFLAKEGS
                     LLAFSAAETVQASLVGAVRGQGKDESKVEDDPKGKEESFSLESDVDYSSDDNLTGQAA
                     HKEEDPGHALEETPPSSGAAGSTTSTGKNRRRRTAFTSEQLLELEKEFHCKKYLSLTE
                     RSQIAHALKLSEVQVKIWFQNRRAKWKRVKAGNANSKTGEPSRNPKIVVPIPVHVSRF
                     AIRSQHQQLEQARP"
     exon            939..2140
                     /gene="GBX2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2091
                     /gene="GBX2"
     regulatory      2108..2113
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GBX2"
                     /note="hexamer: AATAAA"
     polyA_site      2140
                     /gene="GBX2"
                     /note="major polyA site"
ORIGIN      
        1 aaaggtgctg gctgcgcccg ccggagataa gtacgccggc ttcgcgcgct ccccagcggc
       61 ccgcgggagg cgacggacgg cgggacggac ggacggacgg cagcttaccg gggccgaggg
      121 ccaaacccgc gaagcgcgcg ggctccgacg ggcaagcggc ggacgggggc tccggcgagc
      181 cctcggggcc cgggcgccct ctccactccg gggcgcacgg cctcaccccg cacaccccct
      241 gcacccgccc ggcatgcagc gggcccagga ctgaggggcg gaggcgtctg ctctccgggt
      301 cccgctcggc ccctgcccgg cgctcccggc ggtgctcccg gcgtccggcg ggcttcccgg
      361 cggcggcgcg gcgcggggac ttttcgcctc tcgctggcct ctaccgagcg cgtctatgag
      421 cgcagcgttc ccgccgtcgc tgatgatgat gcagcgcccg ctggggagta gcaccgcctt
      481 cagcatagac tcgctgatcg gcagcccgcc gcagcccagc cccggccatt tcgtctacac
      541 cggctacccc atgttcatgc cctaccggcc ggtagtgctg ccgccgccgc cgccgccgcc
      601 gcccgcgctg ccccaggccg cgctgcagcc agcgctgccg cccgcacacc ctcaccacca
      661 gatccccagc ctgcccacag gcttctgctc cagcctggcg cagggcatgg cgctcacctc
      721 tacgctcatg gccacgctcc ccggcggctt ctccgcgtcg ccccagcacc aggaggcggc
      781 agcggcccgc aagttcgcgc cgcagccgct gcccggcggc ggtaacttcg acaaggcgga
      841 ggcgctgcag gctgacgcgg aggacggcaa aggcttcctg gccaaagagg gctcgctgct
      901 cgccttctcc gcggccgaga cggtgcaggc ttcgctcgtc ggggctgtcc gagggcaagg
      961 gaaagacgag tcaaaggtgg aagacgaccc gaagggcaag gaggagagct tctcgctgga
     1021 gagcgatgtg gactacagct cggatgacaa tctgactggc caggcagctc acaaggagga
     1081 agacccgggc cacgcgctgg aggagacccc gccgagcagc ggcgccgcgg gcagcaccac
     1141 gtctacgggc aagaaccggc ggcggcggac tgccttcacc agcgagcagc tgctggagct
     1201 agagaaggag ttccactgca aaaagtacct ctccttgacc gagcgctcgc agatcgccca
     1261 cgccctcaaa ctcagcgagg tgcaggtgaa aatctggttc cagaaccgac gggccaagtg
     1321 gaaacgggtg aaggcaggca atgccaattc caagacaggg gagccctccc ggaaccctaa
     1381 gatcgtcgtc cccatccctg tccacgtcag caggttcgct atcagaagtc agcatcagca
     1441 gctagaacag gcccggccct gagggtccag aagggccagg gcctggcacc cacctggaga
     1501 agcccccgca cccgagggaa cccatggtgg actccactgt gtttgaagca acaaagtcac
     1561 agcccagctg tggccatccc aagcaaattg agaatatatt cactaaatgg gcttaaaaga
     1621 ctgcttttga aggggcttac agccacacca gaagacacgc taaatattta ttatactatc
     1681 ctactttgta cataaatatc tctatagact ggatctcagc tgcagtattt tgaaagttat
     1741 aggacttaat tacccagtaa tttctccact cattccagaa gaaaacaaaa cctctgctgt
     1801 aatctgcgcg gttagcagcc acctttccat acttttccaa aggtaaacgt gaaacccaag
     1861 aacaagcact ggggtttaaa ctgattttgg tttgcgttct ggctttttga gttgactgaa
     1921 aagcagacat tgttaggcag cctggaaatt cgctttgctt tgctttgctt ttgcctggct
     1981 ttggtttccc ctggggttgc ctatcctatc tgagccatgt gaagcacgtc tccgttgtgt
     2041 taatttattt caatgtagct tattttctta taagttatga catttaaaca atttcagtct
     2101 tgtgaataat aaaaaggaga gaggggggga aaaagaccaa
//
