LOCUS       NM_001377137            6403 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens golgi brefeldin A resistant guanine nucleotide
            exchange factor 1 (GBF1), transcript variant 4, mRNA.
ACCESSION   NM_001377137 XM_005270261
VERSION     NM_001377137.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Hirano,J., Hayashi,T., Kitamura,K., Nishimura,Y., Shimizu,H.,
            Okamoto,T., Okada,K., Uemura,K., Yeh,M.T., Ono,C., Taguwa,S.,
            Muramatsu,M. and Matsuura,Y.
  TITLE     Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis
            through interaction with GBF1
  JOURNAL   J Virol 98 (7), e0081324 (2024)
   PUBMED   38904364
  REMARK    GeneRIF: Enterovirus 3A protein disrupts endoplasmic reticulum
            homeostasis through interaction with GBF1.
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Navare,A.T., Mast,F.D., Olivier,J.P., Bertomeu,T., Neal,M.L.,
            Carpp,L.N., Kaushansky,A., Coulombe-Huntington,J., Tyers,M. and
            Aitchison,J.D.
  TITLE     Viral protein engagement of GBF1 induces host cell vulnerability
            through synthetic lethality
  JOURNAL   J Cell Biol 221 (11) (2022)
   PUBMED   36305789
  REMARK    GeneRIF: Viral protein engagement of GBF1 induces host cell
            vulnerability through synthetic lethality.
REFERENCE   3  (bases 1 to 6403)
  AUTHORS   Chia,J., Wang,S.C., Wee,S., Gill,D.J., Tay,F., Kannan,S.,
            Verma,C.S., Gunaratne,J. and Bard,F.A.
  TITLE     Src activates retrograde membrane traffic through phosphorylation
            of GBF1
  JOURNAL   Elife 10, e68678 (2021)
   PUBMED   34870592
  REMARK    GeneRIF: Src activates retrograde membrane traffic through
            phosphorylation of GBF1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6403)
  AUTHORS   Sager,G., Szul,T., Lee,E., Kawai,R., Presley,J.F. and Sztul,E.
  TITLE     Modeling the dynamic behaviors of the COPI vesicle formation
            regulators, the small GTPase Arf1 and its activating Sec7 guanine
            nucleotide exchange factor GBF1 on Golgi membranes
  JOURNAL   Mol Biol Cell 32 (5), 446-459 (2021)
   PUBMED   33405949
  REMARK    GeneRIF: Modeling the dynamic behaviors of the COPI vesicle
            formation regulators, the small GTPase Arf1 and its activating Sec7
            guanine nucleotide exchange factor GBF1 on Golgi membranes.
REFERENCE   5  (bases 1 to 6403)
  AUTHORS   Mendoza-Ferreira,N., Karakaya,M., Cengiz,N., Beijer,D.,
            Brigatti,K.W., Gonzaga-Jauregui,C., Fuhrmann,N., Holker,I.,
            Thelen,M.P., Zetzsche,S., Rombo,R., Puffenberger,E.G., De
            Jonghe,P., Deconinck,T., Zuchner,S., Strauss,K.A., Carson,V.,
            Schrank,B., Wunderlich,G., Baets,J. and Wirth,B.
  TITLE     De Novo and Inherited Variants in GBF1 are Associated with Axonal
            Neuropathy Caused by Golgi Fragmentation
  JOURNAL   Am J Hum Genet 107 (4), 763-777 (2020)
   PUBMED   32937143
  REMARK    GeneRIF: De Novo and Inherited Variants in GBF1 are Associated with
            Axonal Neuropathy Caused by Golgi Fragmentation.
REFERENCE   6  (bases 1 to 6403)
  AUTHORS   Garcia-Mata,R. and Sztul,E.
  TITLE     The membrane-tethering protein p115 interacts with GBF1, an ARF
            guanine-nucleotide-exchange factor
  JOURNAL   EMBO Rep 4 (3), 320-325 (2003)
   PUBMED   12634853
  REMARK    GeneRIF: novel interaction between p115 and Golgi-specific
            brefeldin-A-resistant factor 1 (GBF1)
REFERENCE   7  (bases 1 to 6403)
  AUTHORS   Kawamoto,K., Yoshida,Y., Tamaki,H., Torii,S., Shinotsuka,C.,
            Yamashina,S. and Nakayama,K.
  TITLE     GBF1, a guanine nucleotide exchange factor for ADP-ribosylation
            factors, is localized to the cis-Golgi and involved in membrane
            association of the COPI coat
  JOURNAL   Traffic 3 (7), 483-495 (2002)
   PUBMED   12047556
  REMARK    GeneRIF: detailed subcellular localization to the cis-Golgi
REFERENCE   8  (bases 1 to 6403)
  AUTHORS   Verhoeven,K., Villanova,M., Rossi,A., Malandrini,A., De Jonghe,P.
            and Timmerman,V.
  TITLE     Localization of the gene for the intermediate form of
            Charcot-Marie-Tooth to chromosome 10q24.1-q25.1
  JOURNAL   Am J Hum Genet 69 (4), 889-894 (2001)
   PUBMED   11533914
REFERENCE   9  (bases 1 to 6403)
  AUTHORS   Kirchhausen,T.
  TITLE     Three ways to make a vesicle
  JOURNAL   Nat Rev Mol Cell Biol 1 (3), 187-198 (2000)
   PUBMED   11252894
  REMARK    Review article
            Erratum:[Nat Rev Mol Cell Biol 2001 Mar;2(3):216]
REFERENCE   10 (bases 1 to 6403)
  AUTHORS   Mansour,S.J., Herbrick,J.A., Scherer,S.W. and Melancon,P.
  TITLE     Human GBF1 is a ubiquitously expressed gene of the sec7 domain
            family mapping to 10q24
  JOURNAL   Genomics 54 (2), 323-327 (1998)
   PUBMED   9828135
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL160011.35, AL356420.14 and
            AL121928.13.
            
            On Dec 17, 2019 this sequence version replaced XM_005270261.2.
            
            Summary: This gene encodes a member of the Sec7 domain family. The
            encoded protein is a guanine nucleotide exchange factor that
            regulates the recruitment of proteins to membranes by mediating GDP
            to GTP exchange. The encoded protein is localized to the Golgi
            apparatus and plays a role in vesicular trafficking by activating
            ADP ribosylation factor 1. The encoded protein has also been
            identified as an important host factor for viral replication.
            Multiple transcript variants have been observed for this gene.
            [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (4) encodes the longest isoform
            (4).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3449445.1,
                                           SRR14038192.1872814.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369983.5/ ENSP00000359000.4
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               AL160011.35        30855-31104
            251-356             AL160011.35        44252-44357
            357-423             AL160011.35        45373-45439
            424-555             AL356420.14        84215-84346
            556-674             AL356420.14        91420-91538
            675-783             AL356420.14        92007-92115
            784-844             AL356420.14        92622-92682
            845-899             AL356420.14        93764-93818
            900-1047            AL121928.13        3405-3552
            1048-1271           AL121928.13        3872-4095
            1272-1440           AL121928.13        4633-4801
            1441-1652           AL121928.13        5550-5761
            1653-1751           AL121928.13        6388-6486
            1752-1946           AL121928.13        7084-7278
            1947-2136           AL121928.13        7841-8030
            2137-2277           AL121928.13        8622-8762
            2278-2366           AL121928.13        9076-9164
            2367-2569           AL121928.13        10763-10965
            2570-2693           AL121928.13        11749-11872
            2694-2819           AL121928.13        12451-12576
            2820-2902           AL121928.13        12844-12926
            2903-3139           AL121928.13        13584-13820
            3140-3233           AL121928.13        14105-14198
            3234-3410           AL121928.13        14577-14753
            3411-3475           AL121928.13        15077-15141
            3476-3599           AL121928.13        15227-15350
            3600-3671           AL121928.13        15540-15611
            3672-3766           AL121928.13        15750-15844
            3767-3920           AL121928.13        16073-16226
            3921-4146           AL121928.13        20725-20950
            4147-4307           AL121928.13        21638-21798
            4308-4548           AL121928.13        21926-22166
            4549-4754           AL121928.13        22301-22506
            4755-4905           AL121928.13        24650-24800
            4906-5036           AL121928.13        24887-25017
            5037-5138           AL121928.13        25219-25320
            5139-5252           AL121928.13        25615-25728
            5253-5433           AL121928.13        25872-26052
            5434-5562           AL121928.13        26493-26621
            5563-6403           AL121928.13        27422-28262
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32"
     gene            1..6403
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /note="golgi brefeldin A resistant guanine nucleotide
                     exchange factor 1"
                     /db_xref="GeneID:8729"
                     /db_xref="HGNC:HGNC:4181"
                     /db_xref="MIM:603698"
     exon            1..250
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            251..356
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..5843
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /note="isoform 4 is encoded by transcript variant 4;
                     golgi-specific brefeldin A-resistance guanine nucleotide
                     exchange factor 1; BFA-resistant GEF 1"
                     /codon_start=1
                     /product="Golgi-specific brefeldin A-resistance guanine
                     nucleotide exchange factor 1 isoform 4"
                     /protein_id="NP_001364066.1"
                     /db_xref="CCDS:CCDS91331.1"
                     /db_xref="GeneID:8729"
                     /db_xref="HGNC:HGNC:4181"
                     /db_xref="MIM:603698"
                     /translation="MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFG
                     HLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTH
                     EGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEI
                     MQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRA
                     GGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISS
                     ASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQP
                     DLQQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQK
                     EGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTL
                     LGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIIT
                     VENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFP
                     VSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREAS
                     NTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPP
                     RFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQW
                     LRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPV
                     IQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFR
                     KNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGI
                     FLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLS
                     DVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKN
                     IMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSG
                     PEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDE
                     ETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGL
                     LRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGD
                     DWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSD
                     SEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTV
                     GLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEK
                     RGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQ
                     VSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIAC
                     LCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADV
                     GGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAI
                     PESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDP
                     MPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPG
                     SPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPV
                     PLLATPRPTDPIPTSEVN"
     exon            357..423
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            424..555
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            556..674
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            675..783
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            784..844
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            845..899
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1047
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1271
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1440
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1652
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1751
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1946
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2136
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2277
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2366
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2569
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2693
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2694..2819
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2902
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3139
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3233
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3410
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3475
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3476..3599
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3600..3671
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3766
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3767..3920
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3921..4146
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4147..4307
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4308..4548
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4549..4754
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4755..4905
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4906..5036
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5037..5138
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5139..5252
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5253..5433
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5434..5562
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5563..6403
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6382..6387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /note="hexamer: AATAAA"
     polyA_site      6403
                     /gene="GBF1"
                     /gene_synonym="ARF1GEF; CMT2GG; CMTDI2; CMTDIA"
                     /note="major polyA site"
ORIGIN      
        1 agttaccacg tcagtgagct gacagggaag gtacccggct ctactgcccg tcccggacga
       61 ctcatcctgg cggactgtgc agtcccaccc tgacttcggc ctcaatttcc aggaaacagg
      121 ctccttctct tctcccatct gctaccagag ccgggagagc tgctcggaga cgcctccggg
      181 gtgcgggctg gacatgagca gcggctgccg gtcctgggac taggccccgc cattttggat
      241 ccgctgacag gtttgccaag atggtggata agaatattta catcattcaa ggggagatta
      301 acattgtggt tggggccatc aaacgaaatg cccgatggag cacccataca ccactggatg
      361 aagaacggga tcctctgctg catagtttcg gtcatctaaa ggaggtttta aacagtataa
      421 cagaactctc agaaattgag cccaatgtat tccttcgacc ttttctggaa gtgattcgct
      481 ctgaagatac cactggccct atcactggac tggcactcac ctctgtcaac aagttcctgt
      541 cctatgcact catagatccc acccatgagg gcacagcaga gggcatggag aacatggcag
      601 atgctgtcac ccatgctcgt tttgtgggca cggatcctgc cagtgatgaa gttgtcctga
      661 tgaaaatcct tcaggttcta cggactctgc tgctaacccc agtgggtgcc cacctaacca
      721 atgaatctgt gtgtgagatt atgcagtctt gcttccggat ctgctttgaa atgaggctca
      781 gtgagttatt gagaaaatcc gcagagcaca ctctcgtaga catggtgcag ctgctcttca
      841 caaggttacc tcagtttaaa gaagaaccca agaactatgt ggggaccaac atgaagaagc
      901 tgaaaatgag agccggaggc atgagtgatt catccaaatg gaagaaacag aagagatccc
      961 ctcggccccc acgccatatg accaaagtca caccaggttc agagctgccc actcccaatg
     1021 gaaccacctt atcatctaac ctcactggtg gcatgccctt cattgatgtg cccactccca
     1081 tctcctctgc aagttcagaa gctgcctcag cagtggtcag tccctctaca gacagtggcc
     1141 tggaattctc ctcccaaacc acttccaagg aagaccttac tgatctagag caacctggct
     1201 ctccagggta cagcacagct acagagcctg gaagcagtga gctaggtgtt cccgagcagc
     1261 ctgacctcca gcaggaaggg acccatgtgg aaaagtccca gtcagcatct gtggagtcca
     1321 tccctgaagt gttagaggag tgcacgtccc ctgccgacca ctctgactct gcctctgtcc
     1381 atgacatgga ttacgtcaat ccccggggcg tgcgctttac acagtcctcc cagaaagaag
     1441 gcacagcttt ggtcccctat ggtcttccct gcatccgcga gctcttccgc ttcctcatct
     1501 ccctcaccaa tccacacgac cgccataact cagaggttat gattcacatg ggactgcatt
     1561 tgctgacagt ggcccttgag tcagcccctg tagcccagtg ccagaccctc ttgggcctca
     1621 tcaaggatga gatgtgccgt cacttattcc agctactcag catagagcga ctaaaccttt
     1681 atgctgcttc cctgcgagta tgcttcctac tgtttgagag catgcgagag cacctcaagt
     1741 tccaaatgga gatgtacatc aaaaagctta tggagatcat cactgtggag aaccccaaga
     1801 tgccttatga gatgaaggag atggcactgg aggccattgt gcagctctgg cgcatcccca
     1861 gctttgtcac agagctctac atcaactatg attgtgacta ctactgttcc aacctctttg
     1921 aggaactcac aaagctgctg tccaagaatg ccttccctgt gtctggtcaa ctctatacaa
     1981 cacacctact atctcttgat gccctattga cagtgattga cagcaccgag gcccactgcc
     2041 aggctaaagt cctcaacagc ctcacccagc aagagaagaa ggagacagcc agaccaagct
     2101 gtgagatagt agatggcacc cgagaagcta gcaatactga gagaactgcc agcgatggga
     2161 aagctgtagg catggcctca gacatcccag gcctgcatct gccaggtgga gggcggctgc
     2221 caccagaaca tgggaaatca ggatgcagtg atctggagga agctgttgac tctggggctg
     2281 acaaaaagtt tgcccggaag ccaccccgat tttcctgtct cctgccagat ccacgggaac
     2341 taattgaaat taaaaacaaa aagaagctgc taatcactgg cacagagcag ttcaatcaga
     2401 aaccaaagaa ggggattcag tttctgcaag agaaaggcct cctcaccatc ccaatggaca
     2461 acacagaggt tgctcagtgg ctccgagaga accctcggct ggacaagaag atgattggag
     2521 agtttgtgag tgaccgcaaa aacattgacc tgttggagag ctttgtgagc accttcagtt
     2581 ttcagggtct gcgactggac gaagccctcc gcctctacct ggaagccttc cgtttgcctg
     2641 gggaagcacc agtcatccag aggttgctgg aggcattcac agagcgttgg atgaattgta
     2701 atggctcccc atttgccaat agcgatgcct gcttttccct ggcctatgct gtcatcatgc
     2761 ttaatactga ccagcacaac cacaatgttc gtaaacagaa tgcacccatg accctggagg
     2821 agtttcgcaa aaatctgaaa ggtgtgaatg gaggcaagga ctttgagcaa gacatcctgg
     2881 aggacatgta ccatgccatc aagaatgagg aaattgtaat gcctgaggag cagacaggct
     2941 tggttcggga gaactatgtg tggaatgtgc tgcttcatcg aggtgccacc cctgagggca
     3001 tattcctgcg tgtgcctact gccagctatg atcttgacct cttcaccatg acctggggcc
     3061 ccactattgc tgctctctct tatgtctttg acaaaagcct tgaggagaca atcatccaga
     3121 aagccatctc aggcttcagg aagtgcgcca tgatctccgc ccactatggc ctcagcgatg
     3181 tgtttgacaa tctcatcatc tctctatgca aattcacagc tctcagcagt gagtctattg
     3241 agaacctgcc cagtgtattt ggaagcaacc ctaaagccca tattgcagcc aagacagtat
     3301 tccatttggc ccatcgtcat ggtgacatcc tgcgggaggg ctggaagaat atcatggagg
     3361 ccatgctgca gctcttccga gcccaactac tgcccaaggc tatgatagag gtagaagatt
     3421 tcgtggatcc caatggcaag atctctctac agcgggaaga gacaccatca aaccgaggag
     3481 agtcaacagt gctgagcttt gtgagctggc taacactgag tggtcctgag cagtctagtg
     3541 ttcggggccc atccactgaa aaccaagagg ccaagagagt ggccttagag tgtataaagc
     3601 aatgtgaccc agaaaaaatg atcacagaaa gcaagttcct ccagctggag tcactacagg
     3661 agctcatgaa ggctctggtc tcagtgacac cagatgaaga gacatatgat gaggaagatg
     3721 ctgctttctg cctagagatg ctgctaagga ttgtgttgga gaacagggat cgtgtgggct
     3781 gtgtgtggca gactgttcga gaccatctat accacctctg tgttcaggca caagatttct
     3841 gcttccttgt ggagcgggca gtggtggggt tgctacgcct ggccattcgg cttctccgga
     3901 gagaagagat cagtgctcag gtgctgctct ccctgcgcat tttgctactg atgaagccca
     3961 gtgtgctatc ccgagtcagc caccaggttg cgtatgggct ccatgaactc ctgaagacca
     4021 atgcagccaa catccactca ggtgatgact gggccacact cttcacactg ctggagtgca
     4081 tcggctcagg tgtgaagcct ccagctgctc tgcaggccac agccagggca gatgcacctg
     4141 atgccggggc ccagtcagat agtgagctcc catcctacca tcagaatgac gtgagcctgg
     4201 atcgagggta cacttccgac tcagaggtct acactgacca tggcaggccg ggcaagatac
     4261 accgatcagc cacagatgcc gatgtggtca acagtggttg gttagtggtt gggaaggatg
     4321 acgttgataa ctccaagcca gggcccagcc gcccaggccc ttcacccctg atcaatcaat
     4381 acagcctaac agtgggactg gatttggggc cacacgacac taagtctctg cttaagtgtg
     4441 tggaatcgct gtccttcatt gtgcgtgatg ctgcccacat cacacctgac aactttgagc
     4501 tctgcgtcaa gactctccgg atctttgtgg aggccagtct gaatggcgga tgcaagtccc
     4561 aggagaaacg tggcaagagt cacaaatatg acagcaaagg gaaccgcttc aagaagaaat
     4621 ccaaagaggg atcaatgctt cgccggcctc gaacctccag ccaacatgcc tctcggggcg
     4681 ggcagagtga tgatgatgag gacgaaggcg tgcctgccag ctaccatacg gtgtctttac
     4741 aggtcagtca ggacttgcta gacctgatgc acaccctgca cacgcgggca gcctctatct
     4801 acagctcatg ggcggaggag caacgccacc tggagacagg tggccagaag attgaagctg
     4861 attctcgcac cctctgggcc cactgctggt gccctttact gcagggtatt gcctgcctgt
     4921 gctgcgatgc ccggcgccag gtacggatgc aggcactgac ctatctgcag cgagcactac
     4981 ttgtacatga tctgcaaaag ctagatgccc tggaatggga gtcctgtttt aacaaggtgc
     5041 tgtttcctct acttaccaag ctcttggaga acatcagccc tgcagatgtg ggtgggatgg
     5101 aggagacccg gatgagggct tccacattgc tctctaaggt cttcctgcag cacctgtctc
     5161 cactgctgtc actctctacc tttgcggccc tctggctcac catcttggac ttcatggaca
     5221 agtacatgca cgcaggctcc agcgacttac tgtcagaggc gatccctgag tctctgaaga
     5281 acatgcttct ggtgatggac acagcggaga ttttccacag tgcagatgca cggggaggcg
     5341 gcccctcggc cctctgggag atcacctggg aacgcattga ctgttttctc cctcacctac
     5401 gagatgaact cttcaagcag accgtcatcc aggaccccat gcccatggag cctcaaggcc
     5461 aaaagcctct cgcctcagcc cacctgactt ccgctgctgg cgacactagg acacctggcc
     5521 atccaccgcc cccagagatt ccatctgagc tgggggcctg tgactttgag aagcccgaga
     5581 gcccccgagc cgccagcagc agctccccag gatcaccagt ggcctcaagc cccagcaggc
     5641 tgagccccac ccccgacggg cctccaccct tggctcagcc cccactgatc ctgcagccct
     5701 tggcctcccc actgcaggtg ggcgtgccac ctatgactct gcccatcatc ctcaaccctg
     5761 cgctcatcga ggccacctca ccagtgcccc tcctggccac accccgcccc acagatccca
     5821 tacccacctc tgaggtcaac taaggcaggt cactcagaga tcaggaccag tgcttcccac
     5881 caggctttcc ttgaccccac ttctggctgt cctgcgggcc acaagctctt caggccaagt
     5941 cagagctgct gttgctgcca cttggatggg gacctgaaaa agagaatgtt gatagcccca
     6001 gctaagaccc ccaatcagct gtgggacctt tttcctcctc tgcgctccat tcctgggggt
     6061 tcagcctgag agtgaactca gctgtcatct gcagcctctg cctccagccc ggcagctctg
     6121 gggaggcatc cgtgtgccgg ccctgcagtg cctgcccacg gtcaggcatt gaaaactaag
     6181 cccaaccact ctgcactttg tttcccactc ccattagccc tgggccacct cctccagttc
     6241 ttcctctttt actaattagt tggtcagttt ggagagttga ctggcaccat ggagggtagg
     6301 caggtggggg ctgggtgggg gactgcccac aggagcatgt acatatggaa aaacagaaca
     6361 acagtggact ttttatgata taataaatgt cttagtacca gca
//
