LOCUS       NM_001242409            7434 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1),
            transcript variant 1, mRNA.
ACCESSION   NM_001242409
VERSION     NM_001242409.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Nishino,T., Abe,T., Kaneko,M., Yokohira,M., Yamakawa,K., Imaida,K.
            and Konishi,H.
  TITLE     GAREM1 is involved in controlling body mass in mice and humans
  JOURNAL   Biochem Biophys Res Commun 628, 91-97 (2022)
   PUBMED   36084556
  REMARK    GeneRIF: GAREM1 is involved in controlling body mass in mice and
            humans.
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   3  (bases 1 to 7434)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 7434)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 7434)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7434)
  AUTHORS   Taniguchi,T., Tanaka,S., Ishii,A., Watanabe,M., Fujitani,N.,
            Sugeo,A., Gotoh,S., Ohta,T., Hiyoshi,M., Matsuzaki,H., Sakai,N. and
            Konishi,H.
  TITLE     A brain-specific Grb2-associated regulator of extracellular
            signal-regulated kinase (Erk)/mitogen-activated protein kinase
            (MAPK) (GAREM) subtype, GAREM2, contributes to neurite outgrowth of
            neuroblastoma cells by regulating Erk signaling
  JOURNAL   J Biol Chem 288 (41), 29934-29942 (2013)
   PUBMED   24003223
  REMARK    GeneRIF: Data indicate that a subtype of GAREM, GAREM2, is
            specifically expressed in the mouse, rat, and human brain.
REFERENCE   7  (bases 1 to 7434)
  AUTHORS   Wang,J., Huo,K., Ma,L., Tang,L., Li,D., Huang,X., Yuan,Y., Li,C.,
            Wang,W., Guan,W., Chen,H., Jin,C., Wei,J., Zhang,W., Yang,Y.,
            Liu,Q., Zhou,Y., Zhang,C., Wu,Z., Xu,W., Zhang,Y., Liu,T., Yu,D.,
            Zhang,Y., Chen,L., Zhu,D., Zhong,X., Kang,L., Gan,X., Yu,X., Ma,Q.,
            Yan,J., Zhou,L., Liu,Z., Zhu,Y., Zhou,T., He,F. and Yang,X.
  TITLE     Toward an understanding of the protein interaction network of the
            human liver
  JOURNAL   Mol Syst Biol 7, 536 (2011)
   PUBMED   21988832
  REMARK    Erratum:[Mol Syst Biol. 2017 Dec 18;13(12):965. doi:
            10.15252/msb.20178107.. PMID: 29254952]
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 7434)
  AUTHORS   Bisson,N., James,D.A., Ivosev,G., Tate,S.A., Bonner,R., Taylor,L.
            and Pawson,T.
  TITLE     Selected reaction monitoring mass spectrometry reveals the dynamics
            of signaling through the GRB2 adaptor
  JOURNAL   Nat Biotechnol 29 (7), 653-658 (2011)
   PUBMED   21706016
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 7434)
  AUTHORS   Bandyopadhyay,S., Chiang,C.Y., Srivastava,J., Gersten,M., White,S.,
            Bell,R., Kurschner,C., Martin,C., Smoot,M., Sahasrabudhe,S.,
            Barber,D.L., Chanda,S.K. and Ideker,T.
  TITLE     A human MAP kinase interactome
  JOURNAL   Nat Methods 7 (10), 801-805 (2010)
   PUBMED   20936779
REFERENCE   10 (bases 1 to 7434)
  AUTHORS   Tashiro,K., Tsunematsu,T., Okubo,H., Ohta,T., Sano,E., Yamauchi,E.,
            Taniguchi,H. and Konishi,H.
  TITLE     GAREM, a novel adaptor protein for growth factor receptor-bound
            protein 2, contributes to cellular transformation through the
            activation of extracellular signal-regulated kinase signaling
  JOURNAL   J Biol Chem 284 (30), 20206-20214 (2009)
   PUBMED   19509291
  REMARK    GeneRIF: Extracellular signal-regulated kinase activation in
            response to epidermal growth factor stimulation is regulated by the
            expression of GAREM in COS-7 and HeLa cells.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009835.14, AK025263.1,
            BC121068.1, AK025660.1, AC015563.11 and AI479499.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001242409.1.
            
            Summary: This gene encodes an adaptor protein which functions in
            the epidermal growth factor (EGF) receptor-mediated signaling
            pathway. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.258763.1,
                                           SRR1803617.223086.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467150 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269209.7/ ENSP00000269209.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-398               AC009835.14        110253-110650
            399-431             AK025263.1         1-33
            432-3146            BC121068.1         1-2715
            3147-3422           AK025660.1         1687-1962
            3423-7352           AC015563.11        130387-134316       c
            7353-7434           AI479499.1         16-97               c
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..7434
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /note="GRB2 associated regulator of MAPK1 subtype 1"
                     /db_xref="GeneID:64762"
                     /db_xref="HGNC:HGNC:26136"
                     /db_xref="MIM:617998"
     exon            1..575
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /inference="alignment:Splign:2.1.0"
     CDS             455..3085
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Grb2-associated and regulator of Erk/MAPK; protein FAM59A;
                     family with sequence similarity 59, member A;
                     GRB2-associated and regulator of MAPK1; GRB2 associated,
                     regulator of MAPK1; GRB2 associated regulator of MAPK1 1;
                     GRB2-associated and regulator of MAPK protein 1"
                     /codon_start=1
                     /product="GRB2-associated and regulator of MAPK protein 1
                     isoform 1"
                     /protein_id="NP_001229338.1"
                     /db_xref="CCDS:CCDS56057.1"
                     /db_xref="GeneID:64762"
                     /db_xref="HGNC:HGNC:26136"
                     /db_xref="MIM:617998"
                     /translation="MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECV
                     EGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEP
                     VQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQA
                     EILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGR
                     FSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNI
                     QTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQF
                     DIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYG
                     NNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSE
                     LPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSD
                     TALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSP
                     TLSYYSSGLHNISVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRL
                     SWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTS
                     PVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASE
                     TSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGD
                     GSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFK
                     LSKLQVKKIMQFINGWRPKI"
     exon            576..716
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /inference="alignment:Splign:2.1.0"
     exon            717..847
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /inference="alignment:Splign:2.1.0"
     exon            848..2020
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2187
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..7434
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3261..3266
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /note="hexamer: AATAAA"
     polyA_site      3282
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /note="major polyA site"
     regulatory      7413..7418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /note="hexamer: AATAAA"
     polyA_site      7434
                     /gene="GAREM1"
                     /gene_synonym="C18orf11; FAM59A; GAREM; Gm944"
                     /note="major polyA site"
ORIGIN      
        1 agtcagtgct gggagcgctg ctatggcgtt tctcagaccc gcggctcctc ctcacacgct
       61 cgaggcggag ggaaggaggg gtaagaggag gaggaggcga gcgggcggcc gccgcagctg
      121 cagacccagg gccagaggag gaggcggagc gggagccgcc gcagccaccc gggccgccgc
      181 cgccgcccgc agccgcgcgg ctgctctgag tcttctccgg gcgtgagggc acgcggggcg
      241 ccccagccat gccccgcagc ggcagctgag ccgggcgccg gccccctccc tccgggccgc
      301 cccagtcgcc gcgccccccc ggctgcccgc ggccggagct gcccggcgcc tcccgcgcgc
      361 cccagaccgc gagcgcgagc agcggcggcc gccgaggcgc gggtggtgcc ggtggcggcg
      421 gcgggggagc gcgggacagg aggcttcggg gaagatggac ccggcgccct cgctgggctg
      481 cagcctcaag gatgtgaagt ggagctcggt ggccgtgccg ctcgacctcc tggtcagcac
      541 ttaccggctg ccccagatcg cgcgcctgga caacggagag tgcgtagaag ggctgcggga
      601 aaatgactat ctgctgattc attcctgccg ccagtggacc accatcactg cccacagctt
      661 ggaggagggt cactatgtca ttgggccaaa gatagagatt ccggtacatt atgcagggca
      721 attcaagctg ctggaacaag accgagatat aaaggagcca gtgcaatatt tcaacagtgt
      781 ggaggaggtg gctaaggcat ttcctgaacg cgtgtacgtc atggaggata tcacattcaa
      841 cgtgaaggtt gcttcaggtg aatgcaatga agacactgaa gtttacaaca tcaccctgtg
      901 tactggggat gaactcactc taatggggca ggcagaaatc ctttatgcaa agacattcaa
      961 ggaaaagtca cgactcaaca caatcttcaa aaagattggg aagctcaatt ccatcagcaa
     1021 gctgggaaaa ggcaaaatgc cgtgcctcat ttgtatgaat caccggacca acgaaagcat
     1081 tagccttcca ttccagtgca agggcagatt tagcacccga agtcccctgg aacttcagat
     1141 gcaagagggc gaacacacca tccgcaacat tgtggagaaa accaggcttc ctgtgaatgt
     1201 gactgtgcca agccctccac cgagaaaccc atacgacctc cacttcatcc gtgaggggca
     1261 ccgctataag tttgtgaaca tccagaccaa gacggtggtg gtttgctgtg tgctgcggaa
     1321 caacaagatc ctccccatgc actttccttt gcacttgact gtccccaagt tcagcctccc
     1381 agaacacctg gtgaagggag agagctggcc cgaaaccctg gtccatcact ggctaggtat
     1441 ctgccaagaa cagttcgaca tcgatgagta ttcacgggct gtccgtgatg tgaaaaccga
     1501 ctggaatgaa gaatgcaaga gccccaagaa gggtcggtgc tctggccaca accacgtgcc
     1561 caattcgctc agctacgccc gcgatgagct cacccagtcc ttccaccgac tctcggtctg
     1621 tgtgtatggc aacaatctcc atggcaacag tgaggtgaac cttcatggtt gcagggacct
     1681 ggggggagat tgggctccct ttcctcatga catcctgccc tatcaggact ctggagatag
     1741 tgggagcgac taccttttcc cagaagctag tgaagaatca gcaggcatcc cgggaaagtc
     1801 agaacttccc tacgaagagc tgtggctgga ggaaggcaag cccagccatc agcctctcac
     1861 tcgctctctg agcgagaaga acagatgtga tcagtttaga ggttctgtcc gatccaaatg
     1921 tgcgacttct cctcttccca tccctgggac tctgggagca gcagtgaagt cttcagatac
     1981 tgccctacct ccacctccag tgcctcccaa atctgaagcc gtcagagaag aatgccggct
     2041 cctgaacgcc ccacctgttc caccccgaag cgcaaagcct ttgtccacca gtccctccat
     2101 ccctcctcgc acagtcaagc cagcgcggca acagactcgc tctcccagcc ccaccttgtc
     2161 ctactattct tcagggctac acaacatcag cgtcactaaa actgacacaa atccttctga
     2221 aagcactcct gtttcctgct atccatgtaa ccgagtgaaa actgattctg tggacctgaa
     2281 atccccgttt ggaagtcctt ctgctgaagc tgtgtcctct cggctctcat ggcctaacca
     2341 ttattcagga gcatcagaaa gccagaccag gagtgacttc ctgctggatc caagcaggag
     2401 ttatagttac cctagacaaa agacgccagg cacaccaaag agaaactgcc cagcaccttt
     2461 tgattttgat ggctgtgagc tcctggccag ccccactagc ccagtcactg cagaattcag
     2521 tagcagcgtc tctggttgtc ccaagtcagc cagctactct ctggagagca cagatgtgaa
     2581 atctcttgca gctggtgtga caaagcagag tacgtcatgc cctgccttac cccccagggc
     2641 tccaaaacta gtggaagaga aggtcgcctc cgaaacatct cctttgcctc tgaaaattga
     2701 tggtgctgag gaagacccca agtctgggtc accagatctc tcggaggacc agtattttgt
     2761 taaaaagggc atgcaggaca tcttctctgc ctcctaccct ttctcatctc cgctccatct
     2821 ccagctggcc cccagatcct gtggcgacgg ttccccatgg cagccacctg ctgacctatc
     2881 aggactctct atagaggaag tgtccaagtc actacggttc attggtttgt ccgaagatgt
     2941 catatcattc tttgttactg aaaagattga tgggaacctg cttgttcagc taacggaaga
     3001 aatcctctca gaggatttca aattgagcaa attgcaggtg aagaagataa tgcaattcat
     3061 taatggctgg aggcccaaaa tatagccaaa taacccccgg ccagcatgga acaaaactga
     3121 tcaatgcgtg tgctagaagg ggtgggctgg gacacaattt catgtttttg cactaaaaac
     3181 cttctctgta aatagggata agagaaactc ttactatgca gattacgttt ttgaatggtg
     3241 aacaggctat tttgtacatc aataaaaatg ctgtacagaa cacttggagg tgtgccttgt
     3301 acgtcactca acaaacactc agcagctgct aaaagaaaaa aaggcatgtg cagagaaatc
     3361 attcttaccc aagtaggttt atgtgagaag gtatgatatt tattacaaaa tagccaaagc
     3421 tggaagacat aaaaatcttt aaaaaaaaaa aaaaaacact tttgaacaag tcccttcttt
     3481 caggagggtt gactttagac aattaactgt gttctgtgct ctgtcattca gattgcttct
     3541 tgctaattgt tttactcttg tgcctgaaaa tgttagtgat acgaattgac actttaatgt
     3601 tgtatgcttg gtggggatct ttttatcaca aggcattttc caagagtcat atgttgataa
     3661 agggatatat gttgtcctta gaaatcacac aaatcatgac cacaataatt tatctgtaac
     3721 tgctgctaat tttattgagc agagaaaaaa gaatatacat atataaacat gtataggtgc
     3781 atacacaata cacccggata tgttttacac ataattttga ttctgatatt ttagataaca
     3841 tctgttcact tttaaacaat ctgttgaggt tttgggactg ttatctacat tgattctctt
     3901 aggaatagtg gacagaatga ctccaaagat tctcagaacc ctgtctcttg aagctcaatc
     3961 ttatttgggt tcatcactga aaacttagaa caaatcagta aagaatcctg tattgaagaa
     4021 ttaagtttga gtttaagccc actcaccgca gggctggttt ttgcatgtaa tactcccatg
     4081 atgtctgggg aaatgacagt ttctgttgaa ttataattct ccggtttaag ggtctccttc
     4141 ccctggtgtc tgatggtgag aggtctgaag agcatttgtc agagtacatt aggcattctc
     4201 aacctacttc atgctttacg agagctgaga aatcagacgc accggccttt attattatat
     4261 atgctacatc tgttgagtat ttttaaaata ccaataagag gacttgttta tttcataaac
     4321 cacttcttcc ctctctttca aagaaaatac attattacca tacttttaca ttagtctgag
     4381 aatttttgct aaatatgatt aagtattcca aaatataatg gcgtcattat tcttgacttt
     4441 tttagagaat atacatctta agactgtatt agttctcaga gcaaggcatc atctactccc
     4501 ctgcacctga gagtctggcc ccagattcca gatgtgcgga cagcccatga ccgaggtgca
     4561 cacagtgccc tcttgaatag gaaggtcagc cagtgaggcg gcagcataac ttgcaggatg
     4621 gacttatatc ttaccatctc ttcctgctac caaaataaaa aaataaattg aaagacaaga
     4681 cccaccagtc tagttttgta gacctgtgaa atgtgtcttt atttcttggc attagaagaa
     4741 agatattttt aatccatatc gtggttttag agttgaagtg aattgatacc caattttcca
     4801 tcttagatct cctttatcaa aaaaaaaagc attttttttc tcctaacgtg tctcccaaaa
     4861 tgtaaatact ataggcaaaa agagtaccct gtaaaattct cacactggag cacctcttca
     4921 aaagtcacag ataactcaat gaggaagtgc tggtactgca ctcacaggac atgagaccta
     4981 tgaagctttc gtgttttgaa agcatccttc tgtgtgaggg ggactgcctc ccttagtaaa
     5041 gtaaacctct cagaacttct ttttaaaata aaaacagaca agatgcagtt actaagctgc
     5101 aagttttcat tggcaaacct agctgaatcc gttttaaaat acttagcact cggacacaat
     5161 tctcaaactc aggaagaagg aacagggttg gtttgggttt actataaagg ttgcctcaca
     5221 taaacaactg actcctttta gcgttttttt tctttttgta aaaactttac acaaaatagg
     5281 gttttaaatt ctttttgctt gttttggact tggtgtattt ttgtcaagtt tatattgatt
     5341 ggcaacgcag agactccttg agggcaggga ctgtggcctt tgattcactt atgctacagt
     5401 ctagtatcac tacagtgctg agtaaaagac acagcccctg ccctcttgga gatcatattg
     5461 tttatatttg aaatctcagt tgctaagagt atgtatgtgt gagtaagaaa gacgtgaagt
     5521 cctatgggca gctgggtata tggagaatta cgtgtgtcct gtctttaaaa cgtcagcctc
     5581 tattaagggg tacagaacac cccaagatca ctaaaacttg gaatgtgttg gaggcagaaa
     5641 gtatctctct cattgcctag tgttgttgtt tttttgtttt ttgttttttg ttttttttac
     5701 tgacctggaa ttcttttaat tgtaataaat atcttctgtc acaaataaat gccacaaata
     5761 aatgaaattg ccattcctaa gctgagcacc tgtctgtgaa ggaccttggt gtcctgactg
     5821 gacagagcat tcatagctcc aagagaatca tgacaagata tgcaaagcag ggataataag
     5881 gagaaagccc ttccctttcg agaagccctt ccctttcaca gcccttccct gcgattctgc
     5941 agtgaaaagt gatgcccaga gatccacaca cagaaccctt ctaaccagcc aggtcaagac
     6001 ttatcatctt atctcctctg gcaggtcccc tgtaatcaaa agagaggaag tgtctatcta
     6061 aacaagaccc ttttgggtcc ttagattcat ggcaaaaatc atcatggccc ccatcagaaa
     6121 agaaaatcag caataaacca agccaagcaa ctaaaatgta taatgtggac ctgactcatg
     6181 ttgggaggca gaattcagac actcacagat caatcttgca agttttctct tctttaagtt
     6241 gagctgctca actttatgaa ctgtgaaatt tacttacatt ctttcaaccc acatatatca
     6301 ttgcagttta tgaagttgca gtgaactgcc cattcagcta aattgcagtg tttattcttg
     6361 tctcatgtcg aaatacaaca atcttatggg aaatattatg acagtccgtt ccaactgttc
     6421 tcagacacat gcataaacag tctctgctca cattccacag ccacctgcat gttatccata
     6481 cagctccctg agaagtccag ggatgggagg tggggtgtgg aaggatggag ggtccttcat
     6541 tttctaaaca ctttgtataa gtcactactc atcgtgccta gcaagtataa gattaacatt
     6601 ttgaataaca aactggaaaa caaataatcc ttcaaagaac ctagcgaact gttcaaagga
     6661 aattttaatt tcagtctacc agaaaagtta aatctgttgt tggacgtagg gggctttcag
     6721 aaacatgcct cttatatcaa tttccactat ctttcaagtt acctaaaggc accaggctgc
     6781 ttgagcaaga agaataaaag gcctagagtg caaattatgt gaagtagagt ttttagattg
     6841 tgggattgta ataatacggg attctcatgg ttacatcatg ttaaatcaca ctgttcagtc
     6901 tttaaaactt ggtgttttaa aatttgtctc attgtgatag ccagaaacaa ggaataaacg
     6961 tgatttcagt ttaaactata tacaaatatc ttctgtaatt ttgctgctct aatttttagg
     7021 ctataaattt tgtaatagag cttatcagat cgcaaatttc ctttgtttac aatctcatgt
     7081 agtagggctc agataatttc ttagctataa accttttctc ccactctcat attgtttcat
     7141 ttaaaatatt taagctaagc ctaaaaattg aaatcagtta agaatttgga gagtttatct
     7201 taaatggaaa tggtgtattc ttttcaaaga attttgtaac catctttaca gtttctttgc
     7261 ttggaaaact tgggttgtta actaccagtt aacaatggca ttactttcca aaacgttatc
     7321 taatgggccc aatgtgtacc tcaagataaa cacagtatgt tatgtctaaa aatttccttg
     7381 tctgttgatt taaatggttc tgaaatgtct gtaataaaga aattttcaaa gttg
//
