LOCUS       NM_001282680            9176 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens GTPase activating protein and VPS9 domains 1 (GAPVD1),
            transcript variant 2, mRNA.
ACCESSION   NM_001282680 XM_005251902
VERSION     NM_001282680.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 9176)
  AUTHORS   Khademi,B., Khorrami,M., Ayromlou,H., Rikhtegar,R., Moghadam,E.A.,
            Tahmasebivand,M., Mousavi,S.R., Kheirollahi,M., Fakhr,F.,
            Alizadeh-Ghodsi,M. and Emamalizadeh,B.
  TITLE     An interdependence between GAPVD1 gene polymorphism, expression
            level and response to interferon beta in patients with multiple
            sclerosis
  JOURNAL   J Neuroimmunol 353, 577507 (2021)
   PUBMED   33548618
  REMARK    GeneRIF: An interdependence between GAPVD1 gene polymorphism,
            expression level and response to interferon beta in patients with
            multiple sclerosis.
REFERENCE   4  (bases 1 to 9176)
  AUTHORS   Ibrahim,H., Reus,P., Mundorf,A.K., Grothoff,A.L., Rudenko,V.,
            Buschhaus,C., Stefanski,A., Berleth,N., Stork,B., Stuhler,K.,
            Kalfalah,F. and Reinke,H.
  TITLE     Phosphorylation of GAPVD1 Is Regulated by the PER Complex and
            Linked to GAPVD1 Degradation
  JOURNAL   Int J Mol Sci 22 (7), 3787 (2021)
   PUBMED   33917494
  REMARK    GeneRIF: Phosphorylation of GAPVD1 Is Regulated by the PER Complex
            and Linked to GAPVD1 Degradation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9176)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   6  (bases 1 to 9176)
  AUTHORS   Kim,H., Oh,H., Oh,Y.S., Bae,J., Hong,N.H., Park,S.J., Ahn,S.,
            Lee,M., Rhee,S., Lee,S.H., Jun,Y., Kim,S.H., Huh,Y.H. and Song,W.K.
  TITLE     SPIN90, an adaptor protein, alters the proximity between Rab5 and
            Gapex5 and facilitates Rab5 activation during EGF endocytosis
  JOURNAL   Exp Mol Med 51 (7), 1-14 (2019)
   PUBMED   31358736
  REMARK    GeneRIF: The findings suggest that SPIN90, as an adaptor protein,
            simultaneously binds inactive Rab5 and Gapex5, thereby altering
            their spatial proximity and facilitating Rab5 activation.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9176)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 9176)
  AUTHORS   Su,X., Kong,C. and Stahl,P.D.
  TITLE     GAPex-5 mediates ubiquitination, trafficking, and degradation of
            epidermal growth factor receptor
  JOURNAL   J Biol Chem 282 (29), 21278-21284 (2007)
   PUBMED   17545148
  REMARK    GeneRIF: EGF-stimulated receptor ubiquitination and trafficking are
            mediated via GAPex-5: GAPex-5-mediated EGFR ubiquitination is
            independent of Rab5 activation
REFERENCE   9  (bases 1 to 9176)
  AUTHORS   Hunker,C.M., Galvis,A., Kruk,I., Giambini,H., Veisaga,M.L. and
            Barbieri,M.A.
  TITLE     Rab5-activating protein 6, a novel endosomal protein with a role in
            endocytosis
  JOURNAL   Biochem Biophys Res Commun 340 (3), 967-975 (2006)
   PUBMED   16410077
REFERENCE   10 (bases 1 to 9176)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL354710.17 and AL627223.23.
            
            On May 17, 2019 this sequence version replaced NM_001282680.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.174962.1, BC114937.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000297933.11/ ENSP00000297933.6
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AL354710.17        50834-50967
            135-183             AL354710.17        57944-57992
            184-300             AL354710.17        84466-84582
            301-517             AL354710.17        87898-88114
            518-1361            AL354710.17        90991-91834
            1362-1448           AL354710.17        94071-94157
            1449-1583           AL354710.17        96421-96555
            1584-1773           AL354710.17        96699-96888
            1774-1934           AL354710.17        101460-101620
            1935-2064           AL354710.17        110441-110570
            2065-2190           AL354710.17        112806-112931
            2191-2364           AL354710.17        115424-115597
            2365-2505           AL354710.17        119086-119226
            2506-2640           AL354710.17        120934-121068
            2641-2760           AL354710.17        121518-121637
            2761-2838           AL354710.17        126026-126103
            2839-3209           AL354710.17        126229-126599
            3210-3297           AL354710.17        130185-130272
            3298-3378           AL354710.17        131227-131307
            3379-3501           AL627223.23        6119-6241
            3502-3631           AL627223.23        8690-8819
            3632-3741           AL627223.23        9595-9704
            3742-3901           AL627223.23        10013-10172
            3902-4089           AL627223.23        13954-14141
            4090-4303           AL627223.23        14944-15157
            4304-4376           AL627223.23        18720-18792
            4377-4574           AL627223.23        19828-20025
            4575-9176           AL627223.23        21906-26507
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..9176
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="GTPase activating protein and VPS9 domains 1"
                     /db_xref="GeneID:26130"
                     /db_xref="HGNC:HGNC:23375"
                     /db_xref="MIM:611714"
     exon            1..134
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            135..183
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            184..300
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            301..517
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     CDS             333..4715
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="isoform 2 is encoded by transcript variant 2;
                     GTPase-activating protein and VPS9 domain-containing
                     protein 1; Rab5-activating protein 6; Rab5 exchange
                     factor"
                     /codon_start=1
                     /product="GTPase-activating protein and VPS9
                     domain-containing protein 1 isoform 2"
                     /protein_id="NP_001269609.1"
                     /db_xref="CCDS:CCDS65131.1"
                     /db_xref="GeneID:26130"
                     /db_xref="HGNC:HGNC:23375"
                     /db_xref="MIM:611714"
                     /translation="MVKLDIHTLAHHLKQERLYVNSEKQLIQRLNADVLKTAEKLYRT
                     AWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSR
                     LRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFE
                     LKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETD
                     PNKLIERFSPSQQEKLFGEKGSDRFRQKVQEMVESNEAKLVALVNKFIGYLKQNTYCF
                     PHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPIN
                     EVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLGKFDKSCVAAFLDVVIGGRAVETPP
                     LSSVNLLEGLSRTVVYITYSQLITLVNFMKSVMSGDQLREDRMALDNLLANLPPAKPG
                     KSSSLEMTPYNTPQLSPATTPANKKNRLPIATRSRSRTNMLMDLHMDHEGSSQETIQE
                     VQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFS
                     LCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQD
                     NLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNIDEDRLQEIAGAAAE
                     NMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELES
                     CSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDS
                     VTSPDMDEITHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPD
                     PPILEGAVGGNEARLPNFGSHVLTPAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMS
                     DPSWNRRPGNEERELPPAAAIGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDEKSD
                     RNRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDDPSPRLSAQAQVAE
                     DILDKYRNAIKRTSPSDGAMANYESTEVMGDGESAHDSPRDEALQNISADDLPDSASQ
                     AAHPQDSAFSYRDAKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKV
                     QIAEAINLQDKNLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQ
                     GLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADD
                     KTAQVEDFLQFLYGAMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDI
                     LRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQC
                     ILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLS
                     GEESYWWMQFTAAVEFIKTIDDRK"
     exon            518..1361
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1448
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1583
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1773
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1934
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2064
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2190
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2364
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2505
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2640
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2760
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2838
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..3209
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3210..3297
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3298..3378
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3379..3501
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3502..3631
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3632..3741
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3742..3901
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..4089
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            4090..4303
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            4304..4376
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            4377..4574
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     exon            4575..9176
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5275..5280
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="hexamer: AATAAA"
     polyA_site      5309
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="major polyA site"
     regulatory      6954..6959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="hexamer: AATAAA"
     polyA_site      6978
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="major polyA site"
     regulatory      9145..9150
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
                     /note="hexamer: ATTAAA"
     polyA_site      9176
                     /gene="GAPVD1"
                     /gene_synonym="GAPex-5; GAPEX5; RAP6"
ORIGIN      
        1 gcagggaagc caacaggcga gtggcgaagg tggcggcagc ggcggcgggg gcagtcaccg
       61 gctagggcga cggctccgag gccgagtggc ggtccgacag agtcctccgt gtcctccccc
      121 gcatgacccc cttggggtaa gtgtactgga gagagttggt tatacattct aggaatctca
      181 aagggtctgg ctctgtcacc caggctggag tgcagtgaca cgatctcagc tcactgtaac
      241 ctccgcctcc cgggttcaag cgatcctccc atctgaccct cccaagtagc tggcattcag
      301 tgatcagcct ttgagccttt cccacattga agatggtgaa actagatatt catactctgg
      361 ctcatcacct caagcaggaa cgcttatatg taaactctga gaaacagctc attcagaggc
      421 tcaatgcaga tgtacttaag acagctgaaa agttgtatcg tacagcatgg attgcgaagc
      481 aacagagaat caatttggat cggcttatca taaccagtgc tgaagcttcc cctgctgaat
      541 gttgccaaca tgccaaaatt ttggaagata cacaatttgt tgatgggtat aagcaattgg
      601 gatttcagga gactgcttat ggagaattct tgagtcgatt gagggaaaat cctcgtctta
      661 ttgcctcctc tttggttgct ggagagaaac ttaatcagga gaacacacaa agtgttattt
      721 acacagtttt tacctccctg tatggcaatt gcatcatgca agaagatgaa agctacctcc
      781 ttcaggtttt gcgatacttg attgaatttg aacttaaaga aagtgacaac cctaggcgac
      841 ttttgaggag aggaacttgt gccttcagca tcttatttaa acttttttct gaaggactgt
      901 tttctgccaa acttttcctc acagccactt tacatgagcc aattatgcaa ctgcttgttg
      961 aagatgaaga tcacctggaa acagatccaa acaagctaat tgagaggttc tctccatctc
     1021 agcaggaaaa actctttgga gagaagggct cagatagatt caggcaaaaa gttcaagaaa
     1081 tggtggagtc caatgaagca aagctagtgg ctttggtgaa caaatttatt ggttatctca
     1141 aacagaacac atattgtttt ccacatagtt taaggtggat cgtgtctcag atgtacaaaa
     1201 ccctctcctg tgtagatagg ctggaagttg gggaggtcag ggcaatgtgt actgatctcc
     1261 tgttggcctg cttcatttgt cctgcagttg tcaatccaga acaatatgga ataatttccg
     1321 atgctcctat taatgaagta gcacgattta atctgatgca ggtaggccgc cttttgcagc
     1381 agttagcaat gactggctct gaagagggag atccccgaac aaagagcagc cttggaaagt
     1441 ttgacaaaag ctgtgttgcc gctttccttg atgttgtgat tgggggccgt gcagtggaga
     1501 cccctccatt gtcttccgtc aatcttctgg aaggattgag cagaactgtg gtttatataa
     1561 cctacagtca gcttattact ctggtgaatt ttatgaagag tgtgatgtct ggagatcaac
     1621 tgagagaaga tagaatggct cttgacaatt tattggcaaa cctacccccg gccaagccag
     1681 gaaaaagtag cagtttagaa atgactccct acaatacacc tcagctatct ccagcaacca
     1741 ctccagcaaa taaaaagaat cgattaccta tagcaactcg gagcagaagc cgcaccaata
     1801 tgctaatgga cctacatatg gaccatgaag gatcatctca agaaaccatt caggaggtgc
     1861 aaccagaaga ggtgttggtc atttccttag gtacaggtcc ccagcttact ccagggatga
     1921 tgtcagaaaa tgaggtccta aacatgcagc tttcggatgg aggacaagga gatgtccctg
     1981 ttgatgaaaa caaactccat ggtaaacctg ataaaacctt gcgcttttcc ctctgcagtg
     2041 ataatctgga aggaatatct gaaggtcctt caaatcgctc caattcagtg tcctccctag
     2101 acctagaagg agagtctgtg tcagaacttg gagcaggacc ttctggcagt aatggagttg
     2161 aagctctaca gctgttagaa catgagcaag ctacaacaca ggataacctt gatgataagc
     2221 taaggaagtt tgaaattcgt gacatgatgg gattaacaga tgatagggac atatcagaaa
     2281 cagtgagtga gacctggagt acagacgtct tgggaagtga ctttgaccct aatattgatg
     2341 aagatcgctt gcaagaaatt gcaggtgctg cagcagagaa catgttaggc agtttgctgt
     2401 gcctcccagg ttcagggtca gtgcttcttg acccctgcac tggttctacc atatcagaga
     2461 caacaagtga agcttggagt gtagaggtat tgccaagtga ctcagaggcc ccagacctaa
     2521 agcaggagga gcgtctgcaa gaactggaga gctgttctgg actgggtagc acatctgatg
     2581 atacggatgt cagggaggtc agttcccgcc ccagcacacc aggcctcagt gttgtgtccg
     2641 gcataagtgc aacctctgag gatattccca ataagattga agacctgaga tctgagtgca
     2701 gctctgattt tgggggtaaa gattctgtca ctagtccaga catggatgaa ataactcacg
     2761 gtgcccacca gctgacctct cctccttctc agtcagagtc tctgctggcc atgtttgatc
     2821 cactgtcttc acatgaaggg gcttctgctg tggtaaggcc aaaggttcac tatgctaggc
     2881 catcgcatcc accaccagat cccccaatcc tggaaggagc tgtgggagga aatgaggcca
     2941 ggttgccaaa ctttggttcc catgttttaa ctccagctga aatggaggca ttcaagcaaa
     3001 ggcattctta ccctgagaga ctagttcgaa gcaggagctc tgatatagta tcttctgtcc
     3061 ggagacccat gagtgacccc agctggaacc ggcgtccagg aaatgaagag cgagaactcc
     3121 ctccagctgc agccattggt gctacttctt tggtggctgc acctcattca tcatcttcat
     3181 ccccgagtaa ggactcctca agaggagaga ctgaagaacg caaagatagc gatgatgaga
     3241 aatcagacag gaacagacct tggtggagaa aacgttttgt ttcagccatg cctaaagctc
     3301 ctataccatt tagaaagaaa gaaaaacaag aaaaagacaa agatgatctg gggcctgaca
     3361 gattctcaac actcacagat gatcccagcc ctagactcag tgcacaagct caggtggctg
     3421 aggatattct ggacaaatac aggaatgcca ttaaacggac cagccccagt gatggagcaa
     3481 tggcaaacta tgaaagtaca gaggttatgg gtgatggtga aagtgcacat gattctcccc
     3541 gtgacgaagc actgcagaac atctcggctg atgatctccc agactctgca agccaagcag
     3601 cccacccgca ggattcagct ttctcttaca gagatgcaaa aaagaaactg aggcttgctc
     3661 tttgctctgc ggactctgtt gccttcccag tgctgaccca ttcaacaagg aatggtttac
     3721 cagaccacac agacccagaa gacaatgaaa ttgtatgctt cttaaaagtt caaatagctg
     3781 aagcaattaa tttacaagat aagaatctaa tggctcaact tcaagaaaca atgcgctgtg
     3841 tgtgccgttt tgataatagg acttgtagga aactgctggc ttcgattgct gaggactaca
     3901 gaaaaagagc cccatatatt gcttatctca ctcgttgtcg acaaggacta cagaccacac
     3961 aggctcacct ggaaaggcta ttgcaaagag ttttgcggga caaagaagtg gccaatcgat
     4021 actttaccac tgtctgtgtg agattactgc ttgagagcaa agaaaagaag atcagggaat
     4081 tcattcaaga ctttcagaaa ctcaccgcag ctgacgataa aactgctcag gtagaagatt
     4141 ttctgcagtt tctttatggt gcaatggccc aggatgtcat atggcaaaac gcgagtgaag
     4201 aacagcttca agatgcacag ctggccattg agcgaagcgt gatgaaccgg attttcaagc
     4261 tcgccttcta ccctaatcaa gatggggaca tacttcgcga ccaggttctt catgaacata
     4321 tccagagatt gtctaaagta gtgactgcaa atcacagagc tcttcagata ccagaggttt
     4381 atcttcgaga agcaccatgg ccatctgcac aatcagaaat caggacaata agtgcttata
     4441 aaaccccccg ggacaaagtg cagtgcatcc tgagaatgtg ctctacgatt atgaacctcc
     4501 tgagcctggc caatgaggac tctgtccctg gagcggatga ctttgttcct gtgttggtgt
     4561 ttgtgttgat aaaggcaaat ccaccctgtt tgctgtctac tgtgcagtat atcagtagct
     4621 tttatgctag ctgtctgtct ggagaggagt cctattggtg gatgcagttc acagcagcag
     4681 tagaattcat taaaaccatc gatgaccgaa agtgaccaag accaaggccc accaaggcag
     4741 cagactgtta atcagacaaa cagatctctg agaaggtgca tcagctgctt tgaaggctga
     4801 agattgtttt gtatgatact gcacagcatc aggcatttta aagcagatct ttactaaaca
     4861 ggttaatgag ctaacaagca ggttctctcg tctttgggct ctttcctttc tgagttgcat
     4921 attctatttt cttgtcccca agtagagact agtactacaa aaagggacca catttttcaa
     4981 gtatttctaa gtataaaaaa caaaacaaaa atctcttagg aaatgtctag acctccattc
     5041 ttggattccc tttctttcct tttattttaa aaaagaacag tacccctctt ttaagatgct
     5101 gtcttacatt aatgagcatc taatggaaag aaggtatgag ttgcactgag gattagaata
     5161 gtggtgcgtt agtggcatta tctataaata cactcaccta aattgaaagc taagaaggaa
     5221 atgtaaatat aatatatatt tatatttgat gtaatatgga catctgcaga ttctaataaa
     5281 caaggactat tgctgatagt aggctgtgac atactgtctt gtgaaatggt ttccttgaca
     5341 aaatttaagc tgagcttaaa agcaaaaaac aaaaagtaca cagaaatatt tattaaaatg
     5401 taatacagtt tattgaactt tctaggtatg gagtttgatg gacagggctg cctttaatga
     5461 gtgtgaaggt cactaagtca cttagacatc tcaccgtgga agtttgtgag cctgcattag
     5521 gagatagact gattaccata catgacataa aaaggaacag tggatagctc atactttatg
     5581 gtggttcttc tcctccgaaa taatatactg cagaaatccc agacagagct ccttacaaac
     5641 ctttaattgt aatatatttt tgatgattat tcacattgaa tgcacagacc aagaattcag
     5701 tgaatgtcat tttttaaaaa actaatttgt attgtctgct ctagtgatac aagttttact
     5761 agtgataaac tattttaatc aaccatacta ttcttatgga aaaaaatatc tattttggca
     5821 ggtttctgtg cctttatttc cctcttctga aaaaaagtct gtgttttcat agtttggttt
     5881 gcattgtata tcaataatta atcaggaatg ggttttggtg cctgaaaaat tggccatgga
     5941 ggcacaccaa agcttcaagc acaagtcttg tacatgggcc atcactgtct ggtttcactt
     6001 cgtgtgtttc ctaaacacat ttagctgctt ttttaacaaa ctcagcccca tacttgagtc
     6061 ccttgttgtt gggagcattt ccaggcatct tttaagggaa ctgtgacaaa cagcctcggg
     6121 cagatgaaca cggaggctct ctgttgtctg tctctgagat ctttgtgtct gggaatgcct
     6181 aaagatttta tttttttttc tttgttttta ttttatttta ttttattttt ttgagacaga
     6241 gtctcaccct gttgcccagg ctggagtgca atggtgcgat cttggctcac tgcaacctcc
     6301 acctcccagt tcaagtgatt cccctgcctc agcctcgcga gtagctagga ctacaggcgc
     6361 atgtcaccaa gcccggctaa tttttgtatt tttagtagaa acggggtttc accatgttgg
     6421 ccaggatgat cctcaatctc ctgacctcgt gatccacccg ccttggcctc ccaaagtgcg
     6481 ggattacaag cgtgaaccac cctgcccagc cagaaactag attttcttta tgcctccacc
     6541 ccttcttatt catttacttt acaataccaa aataacaaat tgcataggag tgtgggatgt
     6601 ggtttctgcc ttctagagag agatcagaat taaactagta ctatgaaatg tccttttgaa
     6661 tgttaggtca agaaatccat gtacagagtc gtgcacttca ttggctgtaa ctgtcaaact
     6721 tgccttgtat tagaagttaa atcccatcgt aaatacatca cgaggccagc tgtgtgattt
     6781 ctgagaccta gatgagagtc catttacttc agcctttgat tcagtaaatg ttaagcagca
     6841 gaagacttcg aatggttgaa attcattgtt acaggattta actcataaac caatgagaga
     6901 tttttttttt ctctgcaatg gttgctaagt ctattccatg ttaacactgt ggaaataaat
     6961 atgaagatgt ggacattgca tcggggctct tttctgtgga agagaaggtg gtgggattgg
     7021 ggtgaagggc tacactggag gcaggagata gggctgtgct gtgttggagc cggggctgag
     7081 ggtagacccc ccagagattt gacatctgtg acacctgtga aatgggttcg ggtgctcatg
     7141 actccatagc tggcggagga gcacagcaca gctgttgcag gggcagctaa ggtttcctct
     7201 gaaaatctgt caggagcaag gactactttg gcattaacca tcactgtttg atttcttgac
     7261 aggtatgtga gatggggagt gatttgattt tttttttttt ttttaataga aaagaggaat
     7321 agttgttccc caaatgtttc ttcctccact gccttccagt actttcttta catgaaagat
     7381 tagtgtaaaa gtacaaaatc agcccaggaa acattcagca gccactagtt actacataga
     7441 atttataaag tggatgttaa atggagagag agttggctca ggtcccctga aatcttgtag
     7501 gattaacttc tctgaggaaa agaaaaatca ggtttgcatt ttgtattaat attctgcttt
     7561 tagaagtaat agaaatacaa gttgaagatt gtctttactt acaaaattat attttaattt
     7621 tttttgagac atagtcttgc tctgtcaccc agtctggagt gcagtgctgt gatctcggct
     7681 cactgtaacc accatctccc agtctctagt gattctcatg gctcagcctc cctggttgct
     7741 cagattacaa gtgtatgcca ccacacccag ctaatttttg tattttttgt agagacaggg
     7801 tttcgccatg ttggccaggc tggtctcgaa ctcctggcct caagtgatcc gcctacctcg
     7861 gcttcccaaa gtgctgggat tataggcgtg agccaccgtg cccaaccaca aaattatatg
     7921 tatatatatt taaacgtgtc aaacattttg ctttctttct ccattttagg taaaatctca
     7981 gccacagtaa ttgctttctt ctgctttttc tttataaaaa cctgcttatg aaggccttct
     8041 gatacagata gttaaatcgc tgtagtagga ggctcaggtt ttctaaggga ttgcaaatgt
     8101 gctgcatggt ttttctattc ctatgtcctg gaagcctttg tttaaagtgg gctttacaaa
     8161 tgtgtccctg aggtgcgtga gcaagagaac aatctcagcc ccaatacggg ggctgtgttc
     8221 ttgccgtaag aatctgtagc tgactggtgt gctgaacccg ggcactcttt ttcctattgt
     8281 ttgccatgga aatttccact ttggaccgag taaggtcaac taggtgtttt aaaattgttt
     8341 ttctcctatg tgattgggtg tgaagaggga aagtaggaat acactcagaa ttattactgg
     8401 attgcataaa agtgatagaa gtgtagtggg gttcacttcc tcaattggtg cttcctagat
     8461 ttttctctcc catctcttcc tgctttgttt ctttaaagaa aaggctgttg tctctctttt
     8521 taaatattta agtctagtga gtttattttg tacgcaattg ctttaatttt aagggaagta
     8581 aatctgaaac tatcttgaag cccaaaggat ttttagtaag tggattaaca gtgatgaggt
     8641 atttgcgatt actttaaatg ccaaaatgct tgagaacttt gccaggttaa aaattactta
     8701 atatatttta gaatgtaggt tagaaagtag taggacaagg aatagcttta ttactcttga
     8761 gaacacttgg ataaatgaaa atatagtttg cttccgtgtt aagttgtggt tgagctatgg
     8821 agtgaccctg gagctgccgc atcccacagg cctgagctca gcaagggttc aattcaagag
     8881 agatgacgtc tccacttgga taatggcaga cagtgtgggt agcggccatg cactagggga
     8941 attcacctct gtcaaattaa tactctcctg tctactggat tcttcttgtg cttcacctgg
     9001 attaaaaagc tgtactgcct cttttgaaac agataaaacc tttctataag tctcaattaa
     9061 tgagcttgca caatcttgta tatgtacagg aaacccctcc tgtctcctaa tgtgattagt
     9121 actgtaaaat attctataaa actgattaaa aggggagagc agtttgattc acaggg
//
