LOCUS       NM_014364               1448 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens glyceraldehyde-3-phosphate dehydrogenase,
            spermatogenic (GAPDHS), mRNA.
ACCESSION   NM_014364
VERSION     NM_014364.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Gill,J.G., Leef,S.N., Ramesh,V., Martin-Sandoval,M.S., Rao,A.D.,
            West,L., Muh,S., Gu,W., Zhao,Z., Hosler,G.A., Vandergriff,T.W.,
            Durham,A.B., Mathews,T.P. and Aurora,A.B.
  TITLE     A Short Isoform of Spermatogenic Enzyme GAPDHS Functions as a
            Metabolic Switch and Limits Metastasis in Melanoma
  JOURNAL   Cancer Res 82 (7), 1251-1266 (2022)
   PUBMED   35149585
  REMARK    GeneRIF: A Short Isoform of Spermatogenic Enzyme GAPDHS Functions
            as a Metabolic Switch and Limits Metastasis in Melanoma.
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1448)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1448)
  AUTHORS   Liu,J., Yang,L., Gong,L., Gu,Y., Wang,Y., Sun,C. and Hou,Y.
  TITLE     Effect of GAPDS overexpression on high glucose-induced oxidative
            damage
  JOURNAL   Biochem Biophys Res Commun 500 (2), 191-195 (2018)
   PUBMED   29626473
  REMARK    GeneRIF: GAPDS overexpression antagonize high glucose-induced
            apoptosis by controlling reactive oxygen species accumulation in
            TM3 cells.
REFERENCE   5  (bases 1 to 1448)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 1448)
  AUTHORS   Westhoff,D. and Kamp,G.
  TITLE     Glyceraldehyde 3-phosphate dehydrogenase is bound to the fibrous
            sheath of mammalian spermatozoa
  JOURNAL   J Cell Sci 110 (Pt 15), 1821-1829 (1997)
   PUBMED   9264469
REFERENCE   7  (bases 1 to 1448)
  AUTHORS   Benham,F.J. and Povey,S.
  TITLE     Members of the human glyceraldehyde-3-phosphate
            dehydrogenase-related gene family map to dispersed chromosomal
            locations
  JOURNAL   Genomics 5 (2), 209-214 (1989)
   PUBMED   2793178
REFERENCE   8  (bases 1 to 1448)
  AUTHORS   Ercolani,L., Florence,B., Denaro,M. and Alexander,M.
  TITLE     Isolation and complete sequence of a functional human
            glyceraldehyde-3-phosphate dehydrogenase gene
  JOURNAL   J Biol Chem 263 (30), 15335-15341 (1988)
   PUBMED   3170585
REFERENCE   9  (bases 1 to 1448)
  AUTHORS   Heinz,F. and Freimuller,B.
  TITLE     Glyceraldehyde-3-phosphate dehydrogenase from human tissues
  JOURNAL   Methods Enzymol 89 Pt D, 301-305 (1982)
   PUBMED   7144574
REFERENCE   10 (bases 1 to 1448)
  AUTHORS   Peterson,R.N. and Freund,M.
  TITLE     Effects of (H +), (Na +), (K +) and certain membrane-active drugs
            on glycolysis, motility, and ATP synthesis by human spermatozoa
  JOURNAL   Biol Reprod 8 (3), 350-357 (1973)
   PUBMED   4349855
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP370184.1, BC036373.2 and
            AC002389.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014364.4.
            
            Summary: This gene encodes a protein belonging to the
            glyceraldehyde-3-phosphate dehydrogenase family of enzymes that
            play an important role in carbohydrate metabolism. Like its somatic
            cell counterpart, this sperm-specific enzyme functions in a
            nicotinamide adenine dinucleotide-dependent manner to remove
            hydrogen and add phosphate to glyceraldehyde 3-phosphate to form
            1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play
            an important role in regulating the switch between different
            energy-producing pathways, and it is required for sperm motility
            and male fertility. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.262758.1,
                                           SRR5189667.118236.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000222286.9/ ENSP00000222286.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BP370184.1         45-55
            12-1445             BC036373.2         6-1439
            1446-1448           AC002389.1         34842-34844
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.12"
     gene            1..1448
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /note="glyceraldehyde-3-phosphate dehydrogenase,
                     spermatogenic"
                     /db_xref="GeneID:26330"
                     /db_xref="HGNC:HGNC:24864"
                     /db_xref="MIM:609169"
     exon            1..139
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..1299
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /EC_number="1.2.1.12"
                     /note="spermatogenic cell-specific glyceraldehyde
                     3-phosphate dehydrogenase 2; spermatogenic
                     glyceraldehyde-3-phosphate dehydrogenase; epididymis
                     secretory protein Li 278"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase,
                     testis-specific"
                     /protein_id="NP_055179.1"
                     /db_xref="CCDS:CCDS12465.1"
                     /db_xref="GeneID:26330"
                     /db_xref="HGNC:HGNC:24864"
                     /db_xref="MIM:609169"
                     /translation="MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEP
                     TPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVV
                     AVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRA
                     VGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNI
                     VSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQ
                     NIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAV
                     KAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGY
                     SHRVVDLLRYMFSRDK"
     exon            140..317
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            318..414
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            415..521
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            522..612
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            613..731
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            732..813
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            814..965
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1128
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1226
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1448
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1429..1434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /note="hexamer: AATAAA"
     polyA_site      1448
                     /gene="GAPDHS"
                     /gene_synonym="GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35"
                     /note="major polyA site"
ORIGIN      
        1 agccctggcg ctccgcacgc acctcggtaa catcacagca ggtccaggcc aatgataacc
       61 ttataagagg ccatgtcgaa gcgcgacatc gtcctcacca atgtcaccgt tgtccagttg
      121 ctgcgacagc cgtgcccggt gaccagagca ccgcccccac ctgagcctaa ggctgaagta
      181 gagccccagc cacaaccaga gcccacacca gtcagggagg aaataaagcc accaccgcca
      241 ccactgcctc ctcaccccgc tactcctcct cctaagatgg tgtctgtggc ccgggagctg
      301 actgtgggca tcaatggatt tggacgcatc ggtcgcctgg tcctgcgcgc ctgcatggag
      361 aagggtgtta aggtggtggc tgtgaatgat ccattcattg acccggaata catggtgtac
      421 atgtttaagt atgactccac ccacggccga tacaagggaa gtgtggaatt caggaatgga
      481 caactggtcg tggacaacca tgagatctct gtctaccagt gcaaagagcc caaacagatc
      541 ccctggaggg ctgtcgggag cccctacgtg gtggagtcca caggcgtgta cctctccata
      601 caggcagctt cggaccacat ctctgcaggt gctcaacgtg tggtcatctc cgcgccctca
      661 ccggatgcac caatgttcgt catgggtgtc aatgaaaatg actataaccc tggctccatg
      721 aacattgtga gcaacgcgtc ctgcaccacc aactgtttgg ctcccctcgc caaagtcatc
      781 cacgagcgat ttgggatcgt ggaagggttg atgaccacag tccattccta cacggccacc
      841 cagaagacag tggacgggcc atcaaggaag gcctggcgag atgggcgggg tgcccaccag
      901 aacatcatcc cagcctccac tggggctgcg aaagctgtga ccaaagtcat cccagagctc
      961 aaagggaagc tgacagggat ggcgttccgg gtaccaaccc cggatgtgtc tgtcgtggac
     1021 ctgacctgcc gcctcgccca gcctgccccc tactcagcca tcaaggaggc tgtaaaagca
     1081 gcagccaagg ggcccatggc tggcatcctt gcctacaccg aggatgaggt cgtctctacg
     1141 gacttcctcg gtgataccca ctcgtccatc ttcgatgcta aggccggcat tgcgctcaat
     1201 gacaatttcg tgaagctcat ttcatggtac gacaacgaat atggctacag tcaccgggtg
     1261 gtcgacctcc tccgctacat gttcagccga gacaagtgaa acgggaaggt cctttctttc
     1321 cttcccaggg gccggggccg gaacatgtgc ctcccgttcc agcatctggc tgcccggggg
     1381 aggaaggaca cccggggcgg gcgccccacg ccgatgggtc catggtgaaa taaaaaacag
     1441 tgctcacg
//
