LOCUS       NM_198141               5553 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens glucosidase alpha, neutral C (GANC), transcript
            variant 1, mRNA.
ACCESSION   NM_198141 XM_370857
VERSION     NM_198141.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Qiu,Z., Chu,Q., Zhang,W., Luo,C. and Quan,S.
  TITLE     Level of neutral alpha-1,4-glucosidase in seminal plasma of Chinese
            men
  JOURNAL   Andrologia 50 (3) (2018)
   PUBMED   29282757
  REMARK    GeneRIF: Results showed that the level of NAG in normal men was
            significantly higher than that in subfertile and infertile men.
            Meanwhile, the level of NAG in subfertile men was significantly
            greater than that in infertile men
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Lei,B., Xing,R., Zhou,X., Lv,D., Wan,B., Shu,F., Zhong,L., Wu,H.
            and Mao,X.
  TITLE     Neutral alpha-1,4-glucosidase and fructose levels contribute to
            discriminating obstructive and nonobstructive azoospermia in
            Chinese men with azoospermia
  JOURNAL   Andrologia 48 (6), 670-675 (2016)
   PUBMED   26610429
  REMARK    GeneRIF: our results indicated that neutral alpha glucosidase and
            fructose levels are contributed to discriminating obstructive and
            nonobstructive azoospermia in Chinese patients based on the
            histological types of testes.
REFERENCE   3  (bases 1 to 5553)
  AUTHORS   Flachsbart,F., Franke,A., Kleindorp,R., Caliebe,A., Blanche,H.,
            Schreiber,S. and Nebel,A.
  TITLE     Investigation of genetic susceptibility factors for human longevity
            - a targeted nonsynonymous SNP study
  JOURNAL   Mutat Res 694 (1-2), 13-19 (2010)
   PUBMED   20800603
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   4  (bases 1 to 5553)
  AUTHORS   Oba-Shinjo,S.M., da Silva,R., Andrade,F.G., Palmer,R.E.,
            Pomponio,R.J., Ciociola,K.M., S Carvalho,M., Gutierrez,P.S.,
            Porta,G., Marrone,C.D., Munoz,V., Grzesiuk,A.K., Llerena,J.C. Jr.,
            Berditchevsky,C.R., Sobreira,C., Horovitz,D., Hatem,T.P.,
            Frota,E.R., Pecchini,R., Kouyoumdjian,J.A., Werneck,L., Amado,V.M.,
            Camelo,J.S. Jr., Mattaliano,R.J. and Marie,S.K.
  TITLE     Pompe disease in a Brazilian series: clinical and molecular
            analyses with identification of nine new mutations
  JOURNAL   J Neurol 256 (11), 1881-1890 (2009)
   PUBMED   19588081
  REMARK    GeneRIF: Analysis of Brazilian Pompe patients showed that sometimes
            the nature of the mutation (alpha-Glucosidases )matched the
            phenotype within this group.
REFERENCE   5  (bases 1 to 5553)
  AUTHORS   Hirschhorn,R., Huie,M.L. and Kasper,J.S.
  TITLE     Computer assisted cloning of human neutral alpha-glucosidase C
            (GANC): a new paralog in the glycosyl hydrolase gene family 31
  JOURNAL   Proc Natl Acad Sci U S A 99 (21), 13642-13646 (2002)
   PUBMED   12370436
  REMARK    GeneRIF: in silico and physical cloning of two alleles of human
            neutral alpha-glucosidase (designated GANC on the human gene map)
REFERENCE   6  (bases 1 to 5553)
  AUTHORS   Usuki,F., Ishiura,S., Nonaka,I. and Sugita,H.
  TITLE     alpha-Glucosidase isoenzymes in normal and acid maltase-deficient
            human skeletal muscles
  JOURNAL   Muscle Nerve 11 (4), 365-371 (1988)
   PUBMED   3135493
REFERENCE   7  (bases 1 to 5553)
  AUTHORS   Preobrazhenskaia,M.E. and Minakova,A.L.
  TITLE     [Comparative study of the alpha-glucosidase activity of lymphocytes
            and granulocytes of human peripheral blood]
  JOURNAL   Vopr Med Khim 30 (1), 77-82 (1984)
   PUBMED   6369775
REFERENCE   8  (bases 1 to 5553)
  AUTHORS   Lukomskaia,I.S., Ushakova,N.A. and Kazlas,E.V.
  TITLE     [Soluble and membrane-bound neutral alpha-glucosidase from the
            human kidney]
  JOURNAL   Biokhimiia 48 (11), 1810-1819 (1983)
   PUBMED   6362728
REFERENCE   9  (bases 1 to 5553)
  AUTHORS   Martiniuk,F. and Hirschhorn,R.
  TITLE     Human neutral alpha-glucosidase C: genetic polymorphism including a
            'null' allele
  JOURNAL   Am J Hum Genet 32 (4), 497-507 (1980)
   PUBMED   6994494
REFERENCE   10 (bases 1 to 5553)
  AUTHORS   Martiniuk,F., Hirschhorn,R. and Smith,M.
  TITLE     Assignment of the gene for human neutral alpha-glucosidase C to
            chromosome 15
  JOURNAL   Cytogenet Cell Genet 27 (2-3), 168-175 (1980)
   PUBMED   6995030
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022468.5 and AC012651.8.
            
            On Mar 23, 2021 this sequence version replaced NM_198141.2.
            
            Summary: Glycosyl hydrolase enzymes hydrolyse the glycosidic bond
            between two or more carbohydrates, or between a carbohydrate and a
            non-carbohydrate moiety. This gene encodes a member of glycosyl
            hydrolases family 31. This enzyme hydrolyses terminal, non-reducing
            1,4-linked alpha-D-glucose residues and releases alpha-D-glucose.
            This is a key enzyme in glycogen metabolism and its gene localizes
            to a chromosomal region (15q15) that is associated with
            susceptibility to diabetes. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK074037.1, BC059406.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318010.13/ ENSP00000326227.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1310              AC022468.5         121501-122810
            1311-1373           AC022468.5         124648-124710
            1374-1482           AC022468.5         126782-126890
            1483-1610           AC022468.5         135991-136118
            1611-1793           AC022468.5         141035-141217
            1794-1839           AC022468.5         145911-145956
            1840-1906           AC022468.5         154846-154912
            1907-2003           AC022468.5         156522-156618
            2004-2184           AC022468.5         158583-158763
            2185-2338           AC022468.5         158993-159146
            2339-2574           AC022468.5         170085-170320
            2575-2701           AC022468.5         174598-174724
            2702-2781           AC022468.5         175663-175742
            2782-2925           AC022468.5         177606-177749
            2926-3022           AC022468.5         178876-178972
            3023-3124           AC012651.8         18717-18818
            3125-3368           AC012651.8         19997-20240
            3369-3433           AC012651.8         21018-21082
            3434-3510           AC012651.8         23406-23482
            3511-3585           AC012651.8         26086-26160
            3586-3699           AC012651.8         28431-28544
            3700-3812           AC012651.8         29711-29823
            3813-3916           AC012651.8         31657-31760
            3917-5553           AC012651.8         32358-33994
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..5553
                     /gene="GANC"
                     /note="glucosidase alpha, neutral C"
                     /db_xref="GeneID:2595"
                     /db_xref="HGNC:HGNC:4139"
                     /db_xref="MIM:104180"
     exon            1..1310
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     CDS             1282..4026
                     /gene="GANC"
                     /EC_number="3.2.1.20"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neutral alpha-glucosidase C"
                     /codon_start=1
                     /product="neutral alpha-glucosidase C isoform 1"
                     /protein_id="NP_937784.2"
                     /db_xref="CCDS:CCDS10084.1"
                     /db_xref="GeneID:2595"
                     /db_xref="HGNC:HGNC:4139"
                     /db_xref="MIM:104180"
                     /translation="MEAAVKEEISLEDEAVDKNIFRDCNKIAFYRRQKQWLSKKSTYQ
                     ALLDSVTTDEDSTRFQIINEASKVPLLAEIYGIEGNIFRLKINEETPLKPRFEVPDVL
                     TSKPSTVRLISCSGDTGSLILADGKGDLKCHITANPFKVDLVSEEEVVISINSLGQLY
                     FEHLQILHKQRAAKENEEETSVDTSQENQEDLGLWEEKFGKFVDIKANGPSSIGLDFS
                     LHGFEHLYGIPQHAESHQLKNTGDGDAYRLYNLDVYGYQIYDKMGIYGSVPYLLAHKL
                     GRTIGIFWLNASETLVEINTEPAVEYTLTQMGPVAAKQKVRSRTHVHWMSESGIIDVF
                     LLTGPTPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPY
                     DAMWLDIEHTEGKRYFTWDKNRFPNPKRMQELLRSKKRKLVVISDPHIKIDPDYSVYV
                     KAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFL
                     WNDMNEPSVFRGPEQTMQKNAIHHGNWEHRELHNIYGFYHQMATAEGLIKRSKGKERP
                     FVLTRSFFAGSQKYGAVWTGDNTAEWSNLKISIPMLLTLSITGISFCGADIGGFIGNP
                     ETELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEHTRLIREAIRERYGLLPYWYS
                     LFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALLVHPVTEPKATTVDVFLPG
                     SNEVWYDYKTFAHWEGGCTVKIPVALDTIPVFQRGGSVIPIKTTVGKSTGWMTESSYG
                     LRVALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKFSFCSSVLINSFADQRGHYPSKC
                     VVEKILVLGFRKEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKLSLNIATDWEVRII
                     "
     exon            1311..1373
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1482
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1610
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1793
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1839
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1906
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2003
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2184
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2338
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2574
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2701
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2781
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..2925
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..3022
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3124
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3125..3368
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3369..3433
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3434..3510
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3511..3585
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3586..3699
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3700..3812
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3813..3916
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     exon            3917..5553
                     /gene="GANC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5530..5535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GANC"
                     /note="hexamer: ATTAAA"
     polyA_site      5553
                     /gene="GANC"
                     /note="major polyA site"
ORIGIN      
        1 gtagccccac cccttgctcc tctaggttca gacgttagtg aagtgaatac tcaccgacgg
       61 tatcggaatg tgccatttgg accggtcggc agcagctacg gttgccggtc ccgcactgaa
      121 aaacgacagt ggtgacgggt gagctcccag aagcagaaga atgacaggca acacctgaag
      181 ccacgcagcc gccgccatct tcacagccgt ggagtgccta ccgaaagcat ttcaccctct
      241 tccggttcgt cccgccttct tccggctctg ctctaagggc gggattatcc gggtcctgga
      301 aacgtcgcgg agcttgtttg ctgtgcggcg tagcggcccc tctctcagac agtcgtctgt
      361 gcgccgtgag actttggacc tactgcgcag gcgtcatcct gttggaacct ggtcagctgg
      421 gttagagaga tcgctacatg ccagcctggc ctgagtcttt tctgtctccc agccgttaaa
      481 ggttttagga ccaggcaggc accccgtgca ggatttggct ctctacttcc gctcgcccca
      541 gcccttcatc ccttctaagt caatgtcaga ctttggggcc agaaagtctg gaaaagccct
      601 aagcctggct gcccaagtgg cgtctagtag tgagttctcc ggtttgggtg gtatttaagc
      661 aaaggctgga attcggcggt ggtggagtac acgcgattcc tagactgggt agtgtaacaa
      721 ccttcaaagg ccccttctag ccctgaagag tacgatgctg cagtacaatc tggctggaga
      781 cctagcctcc agcaaagacc cggccggcac ttcccaccct taaccgaaaa cggagacaga
      841 gagggtcagt tttcaaaatc tgtggcgtga ccccgccact gagatagatc tgcagatgct
      901 tcgtcccagt cagggaggcc gtctcggcat ttccaggttc ttaacgctct tgatttttac
      961 cctctaggac tcattgcgta cacgccctca ccgccgccca gtttcggttc ctaacaaaag
     1021 cacgttcctc atcagccacc cataatcaag acaaatttgc caaataaatc attgtagaaa
     1081 cgctagtttg ggcctgaaaa attccaggag caagagtcaa gatttgtcac tccatgagaa
     1141 tctggagggg actcccttcc cagaaacttg acgatgaagt actggttgta attttagaaa
     1201 gacacccaat cggctttttt aaaagatcgc ccagggccct tgtcctgaga gctgggagct
     1261 ggtcggagtg acagagaagc catggaagca gcagtgaaag aggaaataag tcttgaagat
     1321 gaagctgtag ataaaaacat tttcagagac tgtaacaaga tcgcatttta caggcgtcag
     1381 aaacagtggc tttccaagaa gtccacctat caggcattat tggattcagt cacaacagat
     1441 gaagacagca ccaggttcca aatcatcaat gaagcaagta aggttcctct cctggctgaa
     1501 atttatggta tagaaggaaa cattttcagg cttaaaatta atgaagagac tcctctaaaa
     1561 cccagatttg aagttccgga tgtcctcaca agcaagccaa gcactgtaag gctgatttca
     1621 tgctctgggg acacaggcag tctgatattg gcagatggaa aaggagacct gaagtgccat
     1681 atcacagcaa acccattcaa ggtagacttg gtgtctgaag aagaggttgt gattagcata
     1741 aattccctgg gccaattata ctttgagcat ctacagattc ttcacaaaca aagagctgct
     1801 aaagaaaatg aggaggagac atcagtggac acctctcagg aaaatcaaga agatctgggc
     1861 ctgtgggaag agaaatttgg aaaatttgtg gatatcaaag ctaatggccc ttcttctatt
     1921 ggtttggatt tctccttgca tggatttgag catctttatg ggatcccaca acatgcagaa
     1981 tcacaccaac ttaaaaatac tggtgatgga gatgcttacc gtctttataa cctggatgtc
     2041 tatggatacc aaatatatga taaaatgggc atttatggtt cagtacctta tctcctggcc
     2101 cacaaactgg gcagaactat aggtattttc tggctgaatg cctcggaaac actggtggag
     2161 atcaatacag agcctgcagt agagtacaca ctgacccaga tgggcccagt tgctgctaaa
     2221 caaaaggtca gatctcgcac tcatgtgcac tggatgtcag agagtggcat cattgatgtt
     2281 tttctgctga caggacctac accttctgat gtcttcaaac agtactcaca ccttacaggc
     2341 acacaagcca tgccccctct tttctctttg ggataccacc agtgccgctg gaactatgaa
     2401 gatgagcagg atgtaaaagc agtggatgca gggtttgatg agcatgacat tccttatgat
     2461 gccatgtggc tggacataga gcacactgag ggcaagaggt acttcacctg ggacaaaaac
     2521 agattcccaa accccaagag gatgcaagag ctgctcagga gcaaaaagcg taagcttgtg
     2581 gtcatcagtg atccccacat caagattgat cctgactact cagtatatgt gaaggccaaa
     2641 gatcagggct tctttgtgaa gaatcaggaa ggggaagact ttgaaggggt gtgttggcca
     2701 ggtctctcct cttacctgga tttcaccaat cccaaggtca gagagtggta ttcaagtctt
     2761 tttgctttcc ctgtttatca gggatctacg gacatcctct tcctttggaa tgacatgaat
     2821 gagccttctg tctttagagg gccagagcaa accatgcaga agaatgccat tcatcatggc
     2881 aattgggagc acagagagct ccacaacatc tacggttttt atcatcaaat ggctactgca
     2941 gaaggactga taaaacgatc taaagggaag gagagaccct ttgttcttac acgttctttc
     3001 tttgctggat cacaaaagta tggtgccgtg tggacaggcg acaacacagc agaatggagc
     3061 aacttgaaaa tttctatccc aatgttactc actctcagca ttactgggat ctctttttgc
     3121 ggagctgaca taggcgggtt cattgggaat ccagagacag agctgctagt gcgttggtac
     3181 caggctggag cctaccagcc cttcttccgt ggccatgcca ccatgaacac caagcgacga
     3241 gagccctggc tctttgggga ggaacacacc cgactcatcc gagaagccat cagagagcgc
     3301 tatggcctcc tgccatattg gtattctctg ttctaccatg cacacgtggc ttcccaacct
     3361 gtcatgaggc ctctgtgggt agagttccct gatgaactaa agacttttga tatggaagat
     3421 gaatacatgc tggggagtgc attattggtt catccagtca cagaaccaaa agccaccaca
     3481 gttgatgtgt ttcttccagg atcaaatgag gtctggtatg actataagac atttgctcat
     3541 tgggaaggag ggtgtactgt aaagatccca gtagccttgg acactattcc agtgtttcag
     3601 cgaggtggaa gtgtgatacc aataaagaca actgtaggaa aatccacagg ctggatgact
     3661 gaatcctcct atggactccg ggttgctcta agcactaagg gttcttcagt gggtgagtta
     3721 tatcttgatg atggccattc attccaatac ctccaccaga agcaattttt gcacaggaag
     3781 ttttcattct gttccagtgt tctgatcaat agttttgctg accagagggg tcattatccc
     3841 agcaagtgtg tggtggagaa gatcttggtc ttaggcttca ggaaggagcc atcttctgtg
     3901 actacccact catctgatgg taaagatcag cctgtggctt ttacgtattg tgccaaaaca
     3961 tccatcctga gcctggagaa gctctcactc aacattgcca ctgactggga ggtccgcatc
     4021 atatgacaaa gaactgcccc tggtgatgtg agcagggacc tgcctgcccc tttcaacctt
     4081 tcccctcacc ttttttgaga tttttgctgc aatctgtttg ccttccctga atcaaaataa
     4141 tctttcattc gtcaccatta tactaatgaa caatagattt catgtttcaa aatttcagat
     4201 tttacatgtt aagatgtact aacaatattc cttgtatcaa acatctcctt ttctccctga
     4261 tacatagccc tgagacattt atagcgttca ggagtcttct attgcttcca ttccttcagc
     4321 agggctgcgt gggtctgttt taacgtgggc caagcctacc tgggcagccc atttgccagg
     4381 gcttgcctca ggccatgcag cattggcgct ctggctgcag cagctgagtt gctcaaggcc
     4441 agtgtccaag tggacagcag cctctggtac tccccccagt tatcttccac ccacatggac
     4501 tgggcagagc agccctcttc tgtgtgcact gcatacgctg cagccgtggg agttattctc
     4561 ccctagagat cgacttggca gcacgaagga ttcttttctc tttcatgctt ctcaggctca
     4621 atagtttcta attaatctta aaatccatgt cttttacatt gtttttttaa ttaagtgctg
     4681 tttactaacc aaataatatt tataacatga gtaagctata attaataaca atgaaataaa
     4741 tacccatgta cccaccactg gacttcagaa gtagaactca tgactgggac taggatgagg
     4801 caagggagac cctggccttg ggcacaaaat gtaagggatg ccaaaaaaat acagtaatca
     4861 aagtaagtaa tatttcaatc caatattttt aaaaatcaga attaatgcaa aaaaaaccat
     4921 gatgaacaaa atattaaaat ttaaaataaa gacaggatta gtattactga gttttccttt
     4981 tgtcccaggc tctaatatgg cttggcatgg ggcagaacat tacaacatac cagtcgtgtc
     5041 atggtgccca aggctccaca gacctcagtg gctccctgct gcctgccaca gcatctgttt
     5101 tagcagcctc gactcctcag cactcctcag cacacacctc ttcttatcag gcttcctcca
     5161 cttagcaact tgctaacggc cacctctgtg ccttctgatc cctgggcgcc aatatcctcc
     5221 tgcccttacc atccttccag gcccaactta aatcccactt tcccatgaag cctaactgcg
     5281 tgaacacccc tacccccata cccattagca gtgattttgc ccttccccgt aatgctgtcc
     5341 cacttataac tgtgctctac ttagcattct cagggatcat accttaatgt tttcagtatg
     5401 tctgcgttct cctactagat tgtatgtccc tcaagagcat gttctgtttc tcttctgtct
     5461 gacagagcac tattatacct gactttcagt aactgttagc tgtgattagt tagctggtgg
     5521 atttaattga ttaaaaaatt acgattgaat gta
//
