LOCUS       NM_022041              15159 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens gigaxonin (GAN), transcript variant 1, mRNA.
ACCESSION   NM_022041
VERSION     NM_022041.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Renganathan,B., Zewe,J.P., Cheng,Y., Paumier,J.M., Kittisopikul,M.,
            Ridge,K.M., Opal,P. and Gelfand,V.I.
  TITLE     Gigaxonin is required for intermediate filament transport
  JOURNAL   FASEB J 37 (5), e22886 (2023)
   PUBMED   37043392
  REMARK    GeneRIF: Gigaxonin is required for intermediate filament transport.
REFERENCE   2  (bases 1 to 15159)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 15159)
  AUTHORS   Zhang,X., Guo,Y. and Sun,W.
  TITLE     Two novel pathogenic mutations of GAN gene identified in a chinese
            family with giant axonal neuropathy: a case report
  JOURNAL   Mol Biol Rep 49 (9), 9107-9112 (2022)
   PUBMED   35764747
  REMARK    GeneRIF: Two novel pathogenic mutations of GAN gene identified in a
            chinese family with giant axonal neuropathy: a case report.
REFERENCE   4  (bases 1 to 15159)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 15159)
  AUTHORS   Mir,Y.R., Zeng,X., Taneja,A.K., Hassan,A., Sheth,J. and
            Kuchay,R.A.H.
  TITLE     Giant axonal neuropathy with novel GAN pathogenic variant in a
            patient of consanguineous origin from Poonch Jammu and
            Kashmir-India
  JOURNAL   Mol Biol Rep 48 (2), 1607-1614 (2021)
   PUBMED   33528728
  REMARK    GeneRIF: Giant axonal neuropathy with novel GAN pathogenic variant
            in a patient of consanguineous origin from Poonch Jammu and
            Kashmir-India.
REFERENCE   6  (bases 1 to 15159)
  AUTHORS   Ding,J., Liu,J.J., Kowal,A.S., Nardine,T., Bhattacharya,P., Lee,A.
            and Yang,Y.
  TITLE     Microtubule-associated protein 1B: a neuronal binding partner for
            gigaxonin
  JOURNAL   J Cell Biol 158 (3), 427-433 (2002)
   PUBMED   12147674
  REMARK    Erratum:[J Cell Biol. 2003 Oct 13;163(1):189]
REFERENCE   7  (bases 1 to 15159)
  AUTHORS   Kuhlenbaumer,G., Young,P., Oberwittler,C., Hunermund,G.,
            Schirmacher,A., Domschke,K., Ringelstein,B. and Stogbauer,F.
  TITLE     Giant axonal neuropathy (GAN): case report and two novel mutations
            in the gigaxonin gene
  JOURNAL   Neurology 58 (8), 1273-1276 (2002)
   PUBMED   11971098
  REMARK    Erratum:[Neurology 2002 May 14;58(9):1444]
REFERENCE   8  (bases 1 to 15159)
  AUTHORS   Bomont,P., Cavalier,L., Blondeau,F., Ben Hamida,C., Belal,S.,
            Tazir,M., Demir,E., Topaloglu,H., Korinthenberg,R., Tuysuz,B.,
            Landrieu,P., Hentati,F. and Koenig,M.
  TITLE     The gene encoding gigaxonin, a new member of the cytoskeletal
            BTB/kelch repeat family, is mutated in giant axonal neuropathy
  JOURNAL   Nat Genet 26 (3), 370-374 (2000)
   PUBMED   11062483
REFERENCE   9  (bases 1 to 15159)
  AUTHORS   Flanigan,K.M., Crawford,T.O., Griffin,J.W., Goebel,H.H.,
            Kohlschutter,A., Ranells,J., Camfield,P.R. and Ptacek,L.J.
  TITLE     Localization of the giant axonal neuropathy gene to chromosome
            16q24
  JOURNAL   Ann Neurol 43 (1), 143-148 (1998)
   PUBMED   9450783
REFERENCE   10 (bases 1 to 15159)
  AUTHORS   Opal,P.
  TITLE     GAN-Related Neurodegeneration
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301315
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR002770.1, BC044840.1 and
            AC092139.5.
            
            On Jan 15, 2020 this sequence version replaced NM_022041.3.
            
            Summary: This gene encodes a member of the cytoskeletal BTB/kelch
            (Broad-Complex, Tramtrack and Bric a brac) repeat family. The
            encoded protein plays a role in neurofilament architecture and is
            involved in mediating the ubiquitination and degradation of some
            proteins. Defects in this gene are a cause of giant axonal
            neuropathy (GAN). [provided by RefSeq, Oct 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1780077.1, AF291673.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000648994.2/ ENSP00000497351.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                DR002770.1         15-31
            18-2192             BC044840.1         1-2175
            2193-15159          AC092139.5         152185-165151       c
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.2"
     gene            1..15159
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /note="gigaxonin"
                     /db_xref="GeneID:8139"
                     /db_xref="HGNC:HGNC:4137"
                     /db_xref="MIM:605379"
     exon            1..319
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1946
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kelch-like protein 16; kelch-like family member 16;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="gigaxonin isoform 1"
                     /protein_id="NP_071324.1"
                     /db_xref="CCDS:CCDS10935.1"
                     /db_xref="GeneID:8139"
                     /db_xref="HGNC:HGNC:4137"
                     /db_xref="MIM:605379"
                     /translation="MAEGSAVSDPQHAARLLRALSSFREESRFCDAHLVLDGEEIPVQ
                     KNILAAASPYIRTKLNYNPPKDDGSTYKIELEGISVMVMREILDYIFSGQIRLNEDTI
                     QDVVQAADLLLLTDLKTLCCEFLEGCIAAENCIGIRDFALHYCLHHVHYLATEYLETH
                     FRDVSSTEEFLELSPQKLKEVISLEKLNVGNERYVFEAVIRWIAHDTEIRKVHMKDVM
                     SALWVSGLDSSYLREQMLNEPLVREIVKECSNIPLSQPQQGEAMLANFKPRGYSECIV
                     TVGGEERVSRKPTAAMRCMCPLYDPNRQLWIELAPLSMPRINHGVLSAEGFLFVFGGQ
                     DENKQTLSSGEKYDPDANTWTALPPMNEARHNFGIVEIDGMLYILGGEDGEKELISME
                     CYDIYSKTWTKQPDLTMVRKIGCYAAMKKKIYAMGGGSYGKLFESVECYDPRTQQWTA
                     ICPLKERRFGAVACGVAMELYVFGGVRSREDAQGSEMVTCKSEFYHDEFKRWIYLNDQ
                     NLCIPASSSFVYGAVPIGASIYVIGDLDTGTNYDYVREFKRSTGTWHHTKPLLPSDLR
                     RTGCAALRIANCKLFRLQLQQGLFRIRVHSP"
     exon            320..434
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            435..785
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            786..1003
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1125
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1238
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1388
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1525
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1654
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1764
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..15159
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      15139..15144
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /note="hexamer: AATAAT"
     polyA_site      15159
                     /gene="GAN"
                     /gene_synonym="GAN1; GIG; KLHL16"
                     /note="major polyA site"
ORIGIN      
        1 ggatagcaca ggcacgtccc gggggctcca gcttctgctc agagcgcgga gagccgggcc
       61 gggcgggcgc gcgcgcagga ctcgggccgc tcgaggggtc cggccggacg gtgtcgggag
      121 ccggacccgt cggcagagga gcgggcgccg cgatggctga gggcagtgcc gtgtctgacc
      181 ctcagcacgc cgcgcgtctg ctgcgagcgc tcagctcttt ccgcgaggag tctcgcttct
      241 gcgacgcgca cctggtcctc gacggggagg agatcccggt gcagaagaac atcctggcgg
      301 cggccagccc gtacatcagg acaaagttaa actataatcc tccaaaagat gatggatcaa
      361 cttataagat tgaacttgaa gggatatcgg taatggttat gagagagatc ctggattaca
      421 tcttcagtgg gcagatcagg ctaaatgaag atacaatcca agatgttgtt caggcagctg
      481 acctgctgct actgacggac cttaaaaccc tgtgctgtga gtttttggaa ggctgcattg
      541 ctgctgagaa ctgtattggt atccgtgact ttgcactaca ttactgcctc catcacgttc
      601 attaccttgc cacagaatac ctggagactc atttccgaga cgtcagcagc acggaagaat
      661 tcttagagct gagtcctcaa aagcttaaag aagtgatttc tcttgagaag ttaaacgttg
      721 gcaatgaaag atatgtcttt gaagcagtaa ttcgatggat agcacatgat acagaaataa
      781 gaaaggtcca catgaaggat gttatgtcag ctctgtgggt ttcagggttg gactccagtt
      841 atttacggga acagatgctg aatgaaccat tagtacgaga aattgtcaaa gagtgtagca
      901 atataccgct cagccagccg cagcaagggg aggcgatgct ggccaacttc aaaccccggg
      961 gctactctga gtgcatcgtg actgttggtg gagaagagag agtttcacgg aaacccacag
     1021 cagcgatgcg atgcatgtgc cctctctatg accctaacag gcagctttgg atcgaactgg
     1081 cccctttaag catgccgaga attaaccatg gagttctctc agcagaagga tttttgtttg
     1141 tattcggggg ccaagatgaa aataagcaga ctcttagctc aggagaaaag tatgatccag
     1201 atgcaaatac atggacagca ttgccaccta tgaacgaggc aagacataac ttcggaattg
     1261 tggagataga tgggatgctg tacattttgg gaggagagga tggtgaaaag gagctgattt
     1321 ccatggagtg ttacgatatt tattctaaaa cctggacaaa gcaacctgat ttgaccatgg
     1381 tcagaaagat cggctgctat gcagctatga aaaagaaaat ctacgccatg ggtggaggct
     1441 cctacggaaa gctttttgag tctgtagagt gttatgatcc caggacccag cagtggactg
     1501 ccatatgtcc actaaaagag aggaggtttg gagcggtggc ctgtggagtt gctatggagc
     1561 tgtatgtgtt tgggggagtc cgaagtcgtg aggacgccca gggtagcgag atggtaactt
     1621 gcaagtccga gttctaccat gatgagttta aaaggtggat ctatcttaac gaccagaatt
     1681 tatgcatccc cgccagttcc tcttttgttt atggagctgt acctatagga gccagtattt
     1741 atgttattgg agatcttgat acaggtacca attacgacta cgtgcgtgag tttaaaagaa
     1801 gcacaggaac ctggcaccac actaaaccac tccttccatc cgaccttcgc cgtacaggat
     1861 gtgcagcctt acgcattgcg aattgcaagc ttttccgcct gcagcttcag caaggcttat
     1921 tccgtattcg tgttcattcc ccttgaggag gaagcagagc agagtgcgag atcctgaccc
     1981 aagagcacca taacatagct ccgaaaggga gagcagagat ggcagctgaa actcactctg
     2041 tgctgggctt tggtatggta actctttggt ggttttatga tgcttacaaa cttgagcttt
     2101 agctcttgtt tgggagaaca cgtaactgtt gaaaaactac ctgggaggag tgagttcctc
     2161 cagttaaatg tggctgtaga tgttggaggc tagggaggct agtaaatatc aaaaggaaaa
     2221 gggagtggga attgctatca tgtaaaatat caaagttaaa atactaaggt gcattttccc
     2281 tgaagggaac tcatgtctga ctgctgtatt caaatacgta gctttggtaa caaacaaaat
     2341 ccgtatatgc aaatcaacat atccaaacat gccaagactg cttttccact gcacttggaa
     2401 ggatatatta tgcctaaccc tgcccaacaa attaaggttt gtgcctaaaa tgttagattg
     2461 gactgtatgc cagttagtct ccatttattc ctagtactct gtcctaagaa tctttttaaa
     2521 actatatcat gatgaattga aatgaagata aaattgctct tttgtaactt tatcttagta
     2581 atgtaaagat tcagtaaatt gatgagtcag gttgcagccc tcatgtgaac tgaaagaagt
     2641 tgctcgcttc tgtgttgact tagatcaaga cacgtcacgc atcctttctg gggtagtacc
     2701 tgtggagccg ggaagggtct cctgcagtgc cattctgcct tctcaatgag caaaaccatt
     2761 ttctaagtat gaggatatta gtgagtagga gattttataa aagaaagacc tgagtcagac
     2821 aaataataaa ggtctgctgt ggctaaaaaa gcaaaaacaa aaaagctatc cagacaaaat
     2881 acatagggat cagaaacttt ttcatataac ctgtctgaaa gcacacaaag tcttgtttac
     2941 tactgtttag ggtttgaaaa cttgtcttag aatgcgaatc tgtgttaaga atctagtctt
     3001 tgtaatagaa gtttcccagt ggcattcact agtgagagtt ttagacaaat tatgttacga
     3061 gtaaagtttg gctggtagaa taaactacct caacttaatt tcattctgtt catagtagag
     3121 caaattaatc ttttcctcct tccctccttt cctatctctc attctcacct caacccactt
     3181 taccccactt ctcattgggg gaaaaatgtc tttcgttcgt ggaacgtatc ttgtaagata
     3241 ttttgttttc cacttgaatt acaccaccct agtgtgaaat cggggacttc aaagtgcttg
     3301 attccttcct aaattgaatt ttatgcaatg tcaaatttct aatcaattta atatacagta
     3361 cttcattttt aattgttttc taaaaaaaag gttttttttt tccttttgga atatggctgt
     3421 aagaagcagc attttgtctt attaacacat gtataaaggt atcctttggt tttaagtcga
     3481 gaacaggaat ttcttctaga aacttcctgt atggaacgtt tatatgcaat taacatgcat
     3541 atgaaaaaca tcacttacgt tctcccaccc atagacataa aaaagggtga cctgtgattt
     3601 tttttcttat acctgctgga agtcagtaca atgcgtcaaa agttgctgaa agatgctgtg
     3661 cctatggggt tctagagagt gttgagtgtg gtacattaac tctccatttt agccagaaga
     3721 tacctgataa gagaaggtgt aaggttttta tcttatatgc cagaggcacc aggccagatg
     3781 tgccgcacag tcatgtaatt caatcatgtt taatatgtca gtcaaaacca aattcagaat
     3841 tgttttgtgt accagtcagc tgttgtctgc acattttcgt agtgacagtg tggagatctt
     3901 tttaatgaaa gcaaactatg tggcctctgc aaagaaagga agatttttct ttttacaact
     3961 agatattagt tttagaggaa ggaaatagct gaaaaactaa atttgctttg gtgaaatgtc
     4021 ctgtacagaa cagtaccttg gcattcagca gctgtaattg gggaacatta aaacagtaac
     4081 tgacatccag ttaaagccac gatcgtcagc aattctcctt ttttaatttc tgatatttaa
     4141 agtttttttt ccagtctaca ccaggcctct ccaaggagac agttcattat ttaggagtga
     4201 atgtgttcct cttgcaatat tatcagtacc tgcatgactt ggtaaattca ttttataaaa
     4261 atagtgtttt ttttttttaa tttcagttca ttgactctat aactgcagaa attagataat
     4321 gttttataaa ataaatttgc cacataatat gggatgcaat aaccaacaaa gctgctaagt
     4381 gccaaactgt tattttacta tatataaata ttaaaatatt gtgttgaagt atagggatgt
     4441 atttaatttt actatgctcc aacattaatc atgactcttt tgtaaattac agttatttca
     4501 gtattgtaaa ataaatgttg actcatttca tgcaggtttg tgttttaaca ttccacttat
     4561 gccttgaaac atcatttctt gataactttt tgatacttct ttcttgataa ggcactttta
     4621 ccaggtgtgt gttggaattg gtggctcagg ataggtctta aattttaaat acaaatatct
     4681 ttgggggaag tgcaatttat tttcagaagg aaagttgtct caggttagaa taagtaaata
     4741 tttaaagaac ctctcctagg ctgcagtcta aaataggtag tttgtttctt tgtttagcat
     4801 atccaaattt agatttttca aagatttaag tgtttattga aagtgttttt tgttttgttt
     4861 ttgatagtgc ttatatcctg tgaatagaaa aagtgtgaga gatgaatgag tgagttgtac
     4921 gtgtgtgtgt gatgctattt gacctgataa atgtatttgt gtttactttt tgaattgtag
     4981 ttctaaacta catatattac tgtctcctgg tgcttcagat aaattcttga gcctgttgac
     5041 cttcagttta tactactgtg tcgaactcat attgagtgta aatattgaaa tatttgtagt
     5101 ttatagtaag ttttacttgg tgttcgcgtt tctcttcttc agcaggatta aaaacagtgt
     5161 ttaccaactc gtagttggta tactgtcagg atttggccta agaaatttct gaatgcattg
     5221 tttagcttag tacttcattg ctgtaaggaa ttgatagcta actgtaggct tttttcaaac
     5281 tgtataacaa ccaaaaagga gcatttgggg gtatgttgga tggtgttttg tttgtaaaca
     5341 ctttgttata ttgcactacc actttaatga tgacacttcc ttcattgatg gcttggaagt
     5401 gtcattttta taatttatga gttgggggga ttccctagtc aatgcatagg aaatacattc
     5461 tttgtacaaa cgtggtaaag agaggactgg ctttgctatg gcacctaagt tactcatggg
     5521 tagttaagtt tcatttctag agaaatgtct tagctgctgt ggtccattag agttcccttt
     5581 ggtacattcc tacctcagtg taccaaacca gggattccaa aaaccttcca aggagtgcta
     5641 gaaacaccgc agtatcccct tccagaatgt tggcgttcat gattcaagag gcctcccgtg
     5701 gtcagatgaa gacctttggt taaatggggg aagaatgagg tggcgctttg actgtatcaa
     5761 tttaaagatg ccggaacccc gttaaaaaca cctggtggaa gggaaaggtg aggcggccct
     5821 tttccactgg ctgctcactg agcactggag tccaggaagt cagtgagaag agagcagctg
     5881 agtgtagcaa aacccgcctt gggtcagcca acacatgtct gatgagcacc cactaggaac
     5941 tgtgcttgcc atcctgggag ctgaacagtg aggcagaggt cacaccactc tcctactcaa
     6001 ggtcataaac ccactatgga acgggcaagg gccccgtctt tgtcatctgc cagtctcagt
     6061 gcttgcacag agtaggtagg caataaacat ttgacaaata aatataagat tcttgtgatc
     6121 aaagtaatgt tcttctgttg aaaagggcac tccaagagtg actgatttta ctgggttttc
     6181 agtgagaaac tttgtaccca tatgggctgg cctcctatac ggagctgaaa ctagcatggg
     6241 ccggcctcct atatggagct gaaactagta tagcataaac aatgccaagt gcctgatgta
     6301 catgatgtac agtcttctga ttgtaccctc tatatgggac tatgagaccc tctatagact
     6361 cagcacagct tataatgttg atcatgttta gtattgggct ttattgactg ctttttatat
     6421 gggtgtcaaa atctttccag gacatacccc tttgatcctc acacctctgg aataggcagg
     6481 gcactctccc cattgtacca gggtaatctt ggaggcctga tagatatggc ttcatttctc
     6541 actttaggga ggagcaagaa ccaaaagcag aagccaggtc tttggattat cagctcacca
     6601 gtcagccagt gaggctggaa cttgattttt ctaggcatac aagtattttt agtaatagaa
     6661 taatcatttt tagtattaat acaatttacg ctcccaaaca tgtcctttgc tagttgcaca
     6721 ggcataagct gaaagatctt tgattttctt tgttagttag ctataggaag gttaagaatt
     6781 tatttactaa ggtcgtaaga acagaagaaa gtttctttga aagagaaact gtttttttat
     6841 ttcaaagagc ttcatgtata cttccctctc ttattccatg taattagtaa gggaaaataa
     6901 actatgtctt agaatgttga ggaaagtatt ttgatagctt ttaataattc ttgaaatact
     6961 ctttactctg tctgtagttt tgagaaatta cagaaattca tagtataagg atgagaaggg
     7021 gcccatttgc ccatatttga taacacagac ttggattgag tgtctgtcat gcctgctggc
     7081 agttaagtct cagtggtatt gactgctagg cattttctat attttttgca agttagctgg
     7141 taaaacttgg taaagttttg tgtttttcag aaggaaccct attattattt ccattttaaa
     7201 catatttcaa tattgtctca ttgtaatagt tccatttatg tgacatctga ggaattaaat
     7261 catacttcca gatggtttgg tctaatgtat acagtttagg aaagtttatt cttttttatt
     7321 ttattttatt tttttaaatt tcttttagaa actgggtttt gctctgttgc ccaggctgat
     7381 cttaaactcc tggcctcaga tgtggagacc cagctgggac tacaggcatg agccaccacg
     7441 ctccgtagaa agtttgttct ttttcagttc tgtcattgaa attctctaag tgattggatt
     7501 tttaaacccc ttcccctttt catgaaatta aacatcaaat aaataaaact acattatata
     7561 attatttagt cagaaatgac tgttgccctc tctttttttt tttttttttt tttttttttt
     7621 gagacggagt ctcgctctgt cgcctaggct ggagtgcagt ggtgcgatct cggctcactg
     7681 caatctccgc ctcctgggtt caagcaatca tcctgcctca gcctccccag tacctgggat
     7741 tacaggtgct tattaccaca ctcggcgaat tttttgtgtt tttagtagag acggggtttc
     7801 accatgttag ccagcatggt ctcgatctcc taaacttcgt gatccgcccg cctcagcctc
     7861 ccaaagtgct ggattacagg tgtgagccac ctcgcctgcc ctcttttttt ttttttattt
     7921 tgagattaaa actaattctt ttaaagtcac tactcagctg aaatttggcc tgattataaa
     7981 tgttgataat aatttcttcc tggctcaaca aagttgttga gcttccttct gtgcacacat
     8041 tgaaagaaaa ccagaaattg tacagtcgtg atatgaatta gagaaggaac aggtggaaaa
     8101 ggatggcaac acacagagtc cgggggacac acggccttaa gatgaagtga acgagtagac
     8161 actgaatagg atccacgtaa atgaacggcc atttcaaatc attcacatcc caaacggagg
     8221 ttcatctgtc ttctggttgg tgaattatat ttattaggat cttattttta gcatttcatt
     8281 acaagtaata ggtttgggta ccttattttt attgttttta ctcatcagtt atgaataagg
     8341 cataccttag aaacttgggt taaatttttt caagttatac cagtcaactt ggtttaaata
     8401 caactgtact aacttgcttt atcatgtttc ctgtttaatt ggatgagaat tacatgcact
     8461 tattcagttg ttctttgtgt ttataggaaa aactctgttc tcagctacct cattatgtag
     8521 tattgtgctt gatcccctgc caattgagaa gaattcaaca cattctgttc tttaacaaaa
     8581 gcttggaagc aggcagtcag aggccttgag gaatacaatt ctctaaggcc tgttttccat
     8641 tttactactt aaaaaaaaaa aaaaaagaaa agaaaaaaaa gcacttacaa ccaggaggaa
     8701 taataattct cctagagttg ctgcaaactg attacttgaa actcaacttg aactttaaag
     8761 agtctttaaa aggctgtttt cataatgcag aaaagtagtc tatttaaacg ccactgctgt
     8821 gtaaaacagg gggctcgatt tccattcttg ttagcatcag acaagaaaat gtgtctatct
     8881 gtcatctgac ccagtatttg ggatttgggt gcttgaggat actatttttt tcatttctta
     8941 taaggtgcta ttgtttccca gaaatcttta attttgatga aaatctactc attgctaaga
     9001 tcctcctcac ttaaagtgtt tcctcagtgc agagttgagg ttggcagaac tatatagagt
     9061 tggatgatac tgaaaatcat gaatgatgtg atttgttcca tgtatctgtg gaacatccct
     9121 caccttcctc cggtatgcgc cttctgcact ccacacgtgg tcgcctccat cctccctagt
     9181 gtctgtcagc ttgcctggtc accaagtcag gaggaagaag atgaggaggc tcaccagttg
     9241 taggcaagac tcttcctctc gggagatctt gtaataatgg cccagtttca gggcacagtt
     9301 gtacacattc tacttaaatg gaggagttca gtgatgccgt ggccaaaaga gttatgccca
     9361 tttctaaaag cctggcatat ttggtataac ttaagcacca ggtaaaatct ggtgcttaag
     9421 ttgtaccaag tatagccaag tttaactgtc gttgtgtatt aataagattt aatttttatg
     9481 cttgtgcttt tcagagtaaa gggcttgctt tcaatagtag aatgacaaag cttctcctcc
     9541 aatgacaatc tgttagcacc ctgttgactt agctcaggac atgagactga cttagctcag
     9601 gacatgagac atttcctgga agtgatttcc atgagaacaa aggcttattc ctttaactta
     9661 aaccccaaac tttgttattt taattatttt caggcaatca tctttatata ctttccttac
     9721 aaacccagtg gttgagagca taggccttgg tggcaggtgc atctgggctt gggggcttct
     9781 gccacactta ctactctgtg acttgggaag ctcttaaggc tcccaaggct ccgtttctta
     9841 tgtggaagaa gaatagcagg aggactcacc tctgcgggtt gttgtgagga ttaactgggt
     9901 gatgactgta agcagctggg agagtgcctt gtgtgcagtg accactgttg ctgccaccat
     9961 gattatgtca cctctcaaca tggggtggtg ggaggccctg tggatgaggc caaggctgcc
    10021 tgcactgagt gtcccaggcc ttatagcttt ccacattctc acgtctgctg ccacatgata
    10081 tacaagtaaa tgtattagaa gttggtgttc ataaaatact tttttttttt tttttttttg
    10141 agatggagtc tcgctccgtc acccaggctg gagtgcagtg gcgccatctc ccaggctcaa
    10201 gggatcctcc tgcctcggcc tgccaagtag ctgggactac aagtgcacac cagtatgccc
    10261 agctaatttt ttgtattttt agaacaggtg tggtttcacc atattgccca ggctggtctc
    10321 gagctcctga gctcaggcaa tccgcctgcc tcaacctccc aaagtgctgg gattacaggt
    10381 gtgagcatcc agccagaaaa tgatttgctt tctagaccca tatcacctct ctccatctac
    10441 atctctagta tttaaattca acacaattct agatagctga aacagcccaa aatatgtgtt
    10501 tagaatactt agagtttgtt ataaataacc ctctgtttag aatttgccca gtgaaattct
    10561 ctaataatac ttaaaactct ttactaaagt tgaattttcc ttttttttca ttgaaatagc
    10621 aaaggtagta atctgtattg gtcaaagaaa cgagataatt cctagatctc tgttttagac
    10681 cagtagacct aactgtgctt tcttaacttg gatgttacat gaaacttaaa aacaaacaaa
    10741 tatgtgttac aagtacttgt gtgtacgtgc atgtgtgttt gtgcatgtgt ctgtgtgtgt
    10801 ggaacttttg tacctttgca taaagaaggt tatttaacaa agtggtacaa acagtgaact
    10861 acttaatcct tctatggtat catacacttc ttttcctggt taggttatga gactggcaga
    10921 ggtaggagta tataatgtgc tcagcttgtc ttgatttcaa taagacattt gataaagtca
    10981 ttcttgatct ctgaatgtga aaaggaagta gaacagccgg gcgcggtggc tcacgcctgt
    11041 aatcccagga ctttgggagg ccaaggcggg cagatcacct gaggtcggca gtttgagacc
    11101 agcctgacca acgtggagaa accccgtctc tattaaaaat acaaaattag ccgggcatgg
    11161 tggcacatgt ctgtaatccc agctactcgg gaggctgagg caggagaatc tcttgaaccc
    11221 aggaggcgga ggttgcggtg agccgagatg gtgccgttgc actccagccg ggcaacaagg
    11281 gctaaactcc atctcaaaag aaaagaaaga aagggaagta gaagtaggat ttaacaaagg
    11341 accgagaagt cggctgaata gccaggttca gacagtgttg gcaagaacgg atctttggca
    11401 agatcactta aggtgagcta ctctagcagc atcctgtttg acatttgtat ccataacttg
    11461 aataaaaata tagaaggcaa agaagagtgc tgggcaagat gtgttgggtg ttagatctgc
    11521 aatactcttc atagtgtaat ggctaaatgt aaagtcttac gtctcggttc aagaaagcac
    11581 gttgctatat gaaatttcag aaaaaagttt tgttgcgggg ggtgggatat tgatcatttg
    11641 aacatatgcc agttgtttct cctgccctag gggaggttgg cacggcaacc ttcccactcc
    11701 gtaggtgggt agccttgctg atggggcacg tcgtcaccaa gggacctgat tgttgtcatg
    11761 gttttgtctt gtcctcctcc ctggtgaaag tctactgcag ttttattctc agctctggaa
    11821 ggagagggaa cgagcctctg cctgtgtgtc ctgacccctc ttgccttgtt tttgatctgc
    11881 tcaggccggg tgcagtggct cacgcctgta atcccagcac tttgggaggc aaaggcaggt
    11941 ggatcacttg aggtcaggag ttcaagacta gcctggccaa catggtgaaa ccccgtctct
    12001 actaaaaata caaaaattag ccgagtgtgt tggtgggccc ctgtaatccc agctactcgg
    12061 gaggttgagg gaggagaatc acttgacccc aggaggcaga ggttgaagtg agccaggatt
    12121 gtgccactgt actccagcct gggcgacaga gggggactct gtctcacaaa aaaataaaaa
    12181 attaaaaaaa aaggtctgct cagaaatagg ggaaacacgg gatcaagatc attagagatt
    12241 tataggctgg gatgcacacc atgtcttcag agctagccgg ctgtgtcttc tccccgtttc
    12301 tttcactggt gaataacctt tcgtttgaac cagccttgat tgttctctcc atgttccact
    12361 gtggctcccc atggacactg ctaggtggaa tctctgagaa aacaggcact tcgcagtcgg
    12421 cgcatctttc tctgccagct tagggattgc cgtcaaggtg gaggaagggt attttggcca
    12481 tgttcggagt cgttgttttt actcactctt cttgggatag ctttcccacc ctgccctctg
    12541 ctcctctgtc ccaaccctct tccagcctgg tttttttact gcttgaatta tgtatccaaa
    12601 ataaaaagga aaagaaaggt gcagttgggg cgggttgttg ggggcgagga gaatagccct
    12661 tctggttctt cagaccacat aatggccaga gcatgagcct cacagatccc tgagcagacc
    12721 tgcatactag cactttccac aaccagctgg ccctggctgt gagagtcaga tctgtcccca
    12781 aggctccagg tcagaacttg gacccaggtg ccttctgagg caccgccaag gacacatccc
    12841 agcgccttag gcacctgtag gctagaatga caccttccag gccagcccca gaggccctca
    12901 gggctccccc tccaagccgg gagggcaggc aggcacaggc cccgctcact acctcagcac
    12961 ccagtgcagc cccaggctcc cggacgtgct cggcccggtg gcctcttgtg tgacaggccc
    13021 tctgccatgt ctgtccgcag agccgggtgg ggaccctccg tgtgggtcct cctctgtccc
    13081 tttgcagctc gcattcgcca gagcggagcg cagcccttgt ttccttcttt cctcttcagc
    13141 tctgacttaa aaacacacta tgttccaaat cgttgtggga tcggaaacta gaactgcttg
    13201 tttccaggag agaccacctt ttcatatttt ttttaaaagg aatttgtgct tcaaaagaaa
    13261 ctccaaaaaa taaatttatt gttagtctag catatgctca ttgaaattga cttatgtttt
    13321 attttaaaaa atcatgtagg atctggtaca tggggcgggc aaaagggtat catcagctta
    13381 agaagtaaag aagtgagcct tccatttgtg tgggtgataa catctttttc ctagatcgta
    13441 cctcaaaatt caggctctcc atgaaaacaa agataatctg aataaaaatt gtgtgttggg
    13501 aaatgtatca agtgaccttc agttaagaaa agcacaggct gtttattagc acaacttgct
    13561 tcattaaata attcgtggct ttttctgcca ctggggttct tttcgttttg aattcagctt
    13621 gtgtttggtt cctcggtctt gtaccccttc agttcgccgg cgacgtaatg gtgataccct
    13681 ttgttttttg tatcatacct tttgtgaatt gtgacaacca tcccgtaatc attgtggcct
    13741 cagcagaagt tttgaacaat ctttgcagcc actgttgggg tgtgtaccag cagcctggaa
    13801 gtgaccagca gagctgacaa aagcaccccc tgctctgccc acctccgcag agtcctgtcc
    13861 tcatcagaaa cggtatcgcg gatgctcctc aggtaggcgt aggcagactt cattgtgtgt
    13921 tagcagtgac agtgatgtct gtacctatct gtgagtgaga cccattcatg acacactaga
    13981 aatgtgtaca ttcttttgtt ttccatcacg ggtataggtc ttctgccatg aaagagaact
    14041 atcaaggaaa ttcctgaaaa caagatttgt aaattcgatt tgaccctccc aaattcaggg
    14101 ctgctctttg cagtggtccc tggcccagca ggtggccggc gtggctcctg ttggctcatg
    14161 ccagtgttgt gctggaccct cgagtatgtg actgtcaccc ttgtccaaga ctggttttta
    14221 acagtcttga tttgtcattt tgatgctttg aatactataa ggaaaatgcc acgaatacca
    14281 tataaatacc acaccaatct gttgaacata tctgtgtttt ctgacatggg acctgcagaa
    14341 tagtatttct tttaaatcct gcaactttat tatctagtag aattccaagt aggtctgctt
    14401 agacacatat tcagtgaaga ttgtattgtg tgtgatatta tcctatcttt aataaagtcc
    14461 tactactgaa aaacagggat ttgcaatgaa aatcatagct aaataggcag gtttagaaag
    14521 ttcaaaatat tggaagtgct agaaggctgc aacttgtaaa attgtactcc ccaagttcag
    14581 taggatagta gagaagtcgt gctaagttga tttcattgaa atgtcacgtc tcacatcctg
    14641 tggtccaaag gtactagttt gttttgtgga ataattttct gttcacttgc tgataagaaa
    14701 tttagcagtc agaagtaatt tttaattaat aacctttctt tttaaaagtt gctttatggt
    14761 ttacaagtaa tactgatgca aaaaggattt gttaacctaa attaagtagc acgcactttg
    14821 tttacatgct tcagtatatt gtggggttgg tcccttctca cgaacccagg acctgtgctt
    14881 ggggaacatt ttgccatcat gctgttctct tgtaccaagg atcagtgaaa gtgttttatc
    14941 ttttttatgt agtttgtatt gactggattt tataactgta agaaaacaga aacagagtgt
    15001 tgtattttga tctgaaattt gtgtatgcta aagtaatttt gttttatgta ttggaagttc
    15061 acttaaaaac ttgaaatatt ttctagaagg gtaccacaca aaaggaatca tctttaagct
    15121 gtttaattaa cctaataaaa taatttgatg gggaaggca
//
