LOCUS       NM_003774               5385 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens polypeptide N-acetylgalactosaminyltransferase 4
            (GALNT4), mRNA.
ACCESSION   NM_003774
VERSION     NM_003774.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Wu,Q., Xie,X., Zhang,K., Niang,B., Liu,Y., Zhang,C., Huang,T.,
            Huang,H., Li,W., Zhang,J. and Liu,Y.
  TITLE     Reduced expression of ppGalNAc-T4 promotes proliferation of human
            breast cancer cells
  JOURNAL   Cell Biol Int 45 (2), 320-333 (2021)
   PUBMED   33079401
  REMARK    GeneRIF: Reduced expression of ppGalNAc-T4 promotes proliferation
            of human breast cancer cells.
REFERENCE   3  (bases 1 to 5385)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 5385)
  AUTHORS   Hu,C.Y., You,P., Zhang,J., Zhang,H. and Jiang,N.
  TITLE     MiR-506-3p acts as a novel tumor suppressor in prostate cancer
            through targeting GALNT4
  JOURNAL   Eur Rev Med Pharmacol Sci 23 (12), 5133-5138 (2019)
   PUBMED   31298366
  REMARK    GeneRIF: MiR-506-3p acts as a novel tumor suppressor in prostate
            cancer through targeting GALNT4.
REFERENCE   5  (bases 1 to 5385)
  AUTHORS   Yan,X., Lu,J., Zou,X., Zhang,S., Cui,Y., Zhou,L., Liu,F., Shan,A.,
            Lu,J., Zheng,M., Feng,B. and Zhang,Y.
  TITLE     The polypeptide N-acetylgalactosaminyltransferase 4 exhibits
            stage-dependent expression in colorectal cancer and affects
            tumorigenesis, invasion and differentiation
  JOURNAL   FEBS J 285 (16), 3041-3055 (2018)
   PUBMED   29931806
  REMARK    GeneRIF: GALNT4 stage-dependent expression in colorectal cancer
            progression.GALNT4 expression may contribute to the tumorigenesis
            at the early stage and promote cell migration and invasion at the
            advanced stage of the colorectal cancer.
REFERENCE   6  (bases 1 to 5385)
  AUTHORS   Argueso,P., Tisdale,A., Mandel,U., Letko,E., Foster,C.S. and
            Gipson,I.K.
  TITLE     The cell-layer- and cell-type-specific distribution of
            GalNAc-transferases in the ocular surface epithelia is altered
            during keratinization
  JOURNAL   Invest Ophthalmol Vis Sci 44 (1), 86-92 (2003)
   PUBMED   12506059
REFERENCE   7  (bases 1 to 5385)
  AUTHORS   Takeuchi,H., Kato,K., Hassan,H., Clausen,H. and Irimura,T.
  TITLE     O-GalNAc incorporation into a cluster acceptor site of three
            consecutive threonines. Distinct specificity of GalNAc-transferase
            isoforms
  JOURNAL   Eur J Biochem 269 (24), 6173-6183 (2002)
   PUBMED   12473113
REFERENCE   8  (bases 1 to 5385)
  AUTHORS   Hassan,H., Reis,C.A., Bennett,E.P., Mirgorodskaya,E.,
            Roepstorff,P., Hollingsworth,M.A., Burchell,J.,
            Taylor-Papadimitriou,J. and Clausen,H.
  TITLE     The lectin domain of UDP-N-acetyl-D-galactosamine: polypeptide
            N-acetylgalactosaminyltransferase-T4 directs its glycopeptide
            specificities
  JOURNAL   J Biol Chem 275 (49), 38197-38205 (2000)
   PUBMED   10984485
REFERENCE   9  (bases 1 to 5385)
  AUTHORS   Bennett,E.P., Hassan,H., Mandel,U., Mirgorodskaya,E.,
            Roepstorff,P., Burchell,J., Taylor-Papadimitriou,J.,
            Hollingsworth,M.A., Merkx,G., van Kessel,A.G., Eiberg,H.,
            Steffensen,R. and Clausen,H.
  TITLE     Cloning of a human UDP-N-acetyl-alpha-D-Galactosamine:polypeptide
            N-acetylgalactosaminyltransferase that complements other
            GalNAc-transferases in complete O-glycosylation of the MUC1 tandem
            repeat
  JOURNAL   J Biol Chem 273 (46), 30472-30481 (1998)
   PUBMED   9804815
REFERENCE   10 (bases 1 to 5385)
  AUTHORS   Bennett,E.P., Weghuis,D.O., Merkx,G., van Kessel,A.G., Eiberg,H.
            and Clausen,H.
  TITLE     Genomic organization and chromosomal localization of three members
            of the UDP-N-acetylgalactosamine: polypeptide
            N-acetylgalactosaminyltransferase family
  JOURNAL   Glycobiology 8 (6), 547-555 (1998)
   PUBMED   9592121
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA109687.1, BC036390.2 and
            CR739813.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003774.4.
            
            Summary: This gene encodes a member of the
            UDP-N-acetyl-alpha-D-galactosamine:polypeptide
            N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes.
            GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi
            apparatus by catalyzing the transfer of GalNAc to serine and
            threonine residues on target proteins. They are characterized by an
            N-terminal transmembrane domain, a stem region, a lumenal catalytic
            domain containing a GT1 motif and Gal/GalNAc transferase motif, and
            a C-terminal ricin/lectin-like domain. GalNAc-Ts have different,
            but overlapping, substrate specificities and patterns of
            expression. In vitro, the encoded protein can complement other
            GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem
            repeat and can O-glycosylate the P-selectin glycoprotein ligand-1
            molecule. The coding region of this gene is contained within a
            single exon. Fusion transcripts, which combine part of this gene
            with the 5' exons of the neighboring POC1B (POC1 centriolar protein
            homolog B) gene, also exist. [provided by RefSeq, Dec 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC036390.2, SRR1803616.203640.1
                                        [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000529983.3/ ENSP00000436604.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-399               DA109687.1         11-409
            400-4574            BC036390.2         366-4540
            4575-5159           CR739813.1         132-716
            5160-5385           BC036390.2         5126-5351
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.33"
     gene            1..5385
                     /gene="GALNT4"
                     /gene_synonym="GALNAC-T4; GALNACT4"
                     /note="polypeptide N-acetylgalactosaminyltransferase 4"
                     /db_xref="GeneID:8693"
                     /db_xref="HGNC:HGNC:4126"
                     /db_xref="MIM:603565"
     exon            1..5385
                     /gene="GALNT4"
                     /gene_synonym="GALNAC-T4; GALNACT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..1984
                     /gene="GALNT4"
                     /gene_synonym="GALNAC-T4; GALNACT4"
                     /EC_number="2.4.1.41"
                     /note="protein-UDP acetylgalactosaminyltransferase 4;
                     pp-GaNTase 4; polypeptide GalNAc transferase 4;
                     UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
                     4; UDP-N-acetyl-alpha-D-galactosamine:polypeptide
                     N-acetylgalactosaminyltransferase 4 (GalNAc-T4)"
                     /codon_start=1
                     /product="polypeptide N-acetylgalactosaminyltransferase 4"
                     /protein_id="NP_003765.2"
                     /db_xref="CCDS:CCDS53817.1"
                     /db_xref="GeneID:8693"
                     /db_xref="HGNC:HGNC:4126"
                     /db_xref="MIM:603565"
                     /translation="MAVRWTWAGKSCLLLAFLTVAYIFVELLVSTFHASAGAGRAREL
                     GSRRLSDLQKNTEDLSRPLYKKPPADSRALGEWGKASKLQLNEDELKQQEELIERYAI
                     NIYLSDRISLHRHIEDKRMYECKSQKFNYRTLPTTSVIIAFYNEAWSTLLRTIHSVLE
                     TSPAVLLKEIILVDDLSDRVYLKTQLETYISNLDRVRLIRTNKREGLVRARLIGATFA
                     TGDVLTFLDCHCECNSGWLEPLLERIGRDETAVVCPVIDTIDWNTFEFYMQIGEPMIG
                     GFDWRLTFQWHSVPKQERDRRISRIDPIRSPTMAGGLFAVSKKYFQYLGTYDTGMEVW
                     GGENLELSFRVWQCGGKLEIHPCSHVGHVFPKRAPYARPNFLQNTARAAEVWMDEYKE
                     HFYNRNPPARKEAYGDISERKLLRERLRCKSFDWYLKNVFPNLHVPEDRPGWHGAIRS
                     RGISSECLDYNSPDNNPTGANLSLFGCHGQGGNQFFEYTSNKEIRFNSVTELCAEVPE
                     QKNYVGMQNCPKDGFPVPANIIWHFKEDGTIFHPHSGLCLSAYRTPEGRPDVQMRTCD
                     ALDKNQIWSFEK"
     regulatory      5355..5360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GALNT4"
                     /gene_synonym="GALNAC-T4; GALNACT4"
                     /note="hexamer: AATAAA"
     polyA_site      5385
                     /gene="GALNT4"
                     /gene_synonym="GALNAC-T4; GALNACT4"
                     /note="major polyA site"
ORIGIN      
        1 gagtttgtga aaagttgtga gccgagcagg aggaagtagg gagagaggag ttgggctgtg
       61 ccggaggccg aggaccgaga gggctcaggt gacccctgga aagcctgggt ggctggaaag
      121 gagcctagcg cctgcatgaa aggaagaacc tgctgggaag tacctgagct cgagctgtgg
      181 gttccgccgc ccttcccctg cgtggtggct tggtggccgc gtctgcgcct cagccctgag
      241 aatccggatg gcggtgaggt ggacttgggc aggcaagagc tgcctgctgc tggcgttttt
      301 aacagtggcc tatatcttcg tggagctctt ggtctctact tttcatgcct ccgcaggagc
      361 cggccgtgcc agggagctgg ggtcaagaag gctctcagac ctccagaaaa atacggagga
      421 tttgtctcga ccgctttata agaagccccc tgcagattcc cgtgcacttg gggagtgggg
      481 gaaagccagc aaactccagc tcaacgagga tgaactgaag cagcaagaag aactcattga
      541 gagatacgcc atcaatattt acctcagtga caggatttcc ctgcatcgac acatagagga
      601 taaaagaatg tatgagtgta agtcccagaa gttcaactat aggacacttc ctaccacctc
      661 tgttatcatt gctttctata acgaagcctg gtcgactttg ctccgtacca ttcacagtgt
      721 tttagaaact tctcctgcag ttcttttgaa agagatcatc ttggtggatg acttgagtga
      781 cagagtttat ttgaagacac aacttgaaac ttacatcagc aatcttgata gagtacgctt
      841 gattaggacc aataagcgag aggggctggt tagggcccgt ctgattgggg ccactttcgc
      901 cactggggac gtcctcactt tcctggattg tcactgtgag tgtaattccg gttggctgga
      961 accgcttttg gaaaggattg ggagagatga aacagcagtt gtgtgtcctg ttatagacac
     1021 aattgattgg aatacttttg aattctatat gcagataggg gagcccatga ttggtgggtt
     1081 tgactggcgt ttaacatttc agtggcattc tgtccccaaa caggaaaggg acaggcggat
     1141 atcaagaatt gaccccatca gatcacctac catggctgga ggactgtttg ctgtcagcaa
     1201 gaaatatttt cagtaccttg gaacgtatga cacaggaatg gaagtgtggg gaggtgaaaa
     1261 ccttgagctg tcttttaggg tgtggcagtg tggtggcaaa ttggagatcc acccgtgttc
     1321 ccacgtgggc catgtgttcc ccaagcgggc accatatgct cgccccaatt tcctacagaa
     1381 tactgctcgg gcagcagaag tttggatgga tgaatacaaa gagcacttct acaatagaaa
     1441 ccctccagca agaaaagaag cttatggtga tatttctgaa agaaaattac tacgagagcg
     1501 gttgagatgc aagagctttg actggtattt gaaaaacgtt tttcctaatt tacatgttcc
     1561 agaggataga ccaggctggc atggggctat tcgcagtaga gggatctcgt ctgaatgttt
     1621 agattataat tctcctgaca acaaccccac aggtgctaac ctttcactgt ttggatgcca
     1681 tggtcaagga ggcaatcaat tctttgaata tacttcaaac aaagaaataa ggtttaattc
     1741 tgtgacagag ttatgtgcag aggtacctga gcaaaaaaat tatgtgggaa tgcaaaattg
     1801 tcccaaagat gggttccctg taccagcaaa cattatttgg cattttaaag aagatggaac
     1861 tatttttcac ccacactcag gactgtgtct tagtgcttat cggacaccgg agggccgacc
     1921 tgatgtacaa atgagaactt gtgatgctct agataaaaat caaatttgga gttttgagaa
     1981 atagagcaca acagcacttt cgtcatgagc tgacagtagt gtcaagaaag tcaaagagcc
     2041 ttaagagcct cagtgaagat tgtattttat tttatcaaaa gccacctagc agtcatctgt
     2101 ggagcactgg aaagctgggg ttcattttgg tatatcacac tgaaactggg tacccagagt
     2161 gctgctgttt aatatttcac aatgccttac ttattggttg ttttatataa gagttttgtc
     2221 aatatggtct cttcttaaaa gaagttgact atgaattgaa acacacaaaa catttaagtg
     2281 ccagacttaa tattaaagaa tgtaaaggtc caagtaaaat gaggtatgat ttatgttgat
     2341 gtgtaagttc accgcacatc ccacttttta acaaaactca tgaatgtgca gtttgagcca
     2401 ttgctatttt gattacatag aatttgtatt tcttttttag ccagcacatt aaattttaga
     2461 ttttattttt taatctaatt tttttctaat caaaaagaaa attgagctta aggcaaaagg
     2521 cctggtttta gagatatgtg taattggaag agggcatttg tttgagtgtg agtttggagg
     2581 cctttttaac atgcagacat acccatattt aaatgaaatg gggagatatt tacattccgt
     2641 actttgtaaa cttgagctat tggacttcac tgatgtatat attaatacct cagattcctc
     2701 tgattttgta agctgtcttc tctgtgaacg tgtttgtgtg tgtagggcat tttctgattg
     2761 cacttcctta agttatgaat gtactagaaa gggactcatc cagaatacta tgcctccctt
     2821 tgttaatgct taatcattta aagtaaacac aattgaagcc tctctgaagt taaacccaac
     2881 tatgtttatt aaaatgtgtg aaactgaaag tgggctaggt tctaccaagg ctgtggaact
     2941 ctcctacgag ttctgctgat caggaaattt aagaatttat cttaaaaatg caaggaaaaa
     3001 agactgcctt ggcaattgtg aatggtgctt tcaatctcct agcaccgagc ctggcactta
     3061 ggcagctttc agtaagtggg tgaatgaatg actgaatgaa tgaatgaatg gctcagctga
     3121 ggaatgtaac tttggtcaag ttattatgat gtgtttgggc ttagttttct cattggtaaa
     3181 atgtgggtgc tggattggat cttaaagatc ccttccagct ctgaaatgct gattgtacag
     3241 tatattcttc ccagattgac tcactgtgca atctttacaa tactttttat cttttcactt
     3301 ttgacatagg taatgttgtt gagcagttga gcaatgttca gtccagttgt gaagctggag
     3361 aagagaaatg ggttttaaaa attaagtgag gggaggccgg gtgcggtggc tcacgcatgt
     3421 aatcccagca ctttgggagg ccaaggcagg tggatcacga ggtcaggaga tccagaccat
     3481 cctggctaac atggtgaaac ctcgtctcta ctaaaaatac aaaaaattag ccagctgtgg
     3541 tggcgggcgc ctgtagtccc agctactcag gaggctgagg caggagaatg gcgtgaacct
     3601 gtgaggcaga gcttacagtg agccgagatc gtgccactgc actccagcct gggcgacaga
     3661 gcaagactct gtctcaaaaa aaaaaaataa taataaaata agtgagctga actcacctga
     3721 agtggtttac ttctgtgggt taagaagttc tagtcagtgt tcatagtcgt ttcgttttga
     3781 taattgttga accaattttg tttttaaaac ctttagactc tgaaagtaat attttgacta
     3841 agaatgtaaa tatttccaaa ctaaattact cgggaagtaa acgctttttt taaaagtatt
     3901 tttactggtt ttataccaat attatatgca gaaatcacag gatgaattta gaattaaatc
     3961 tcaattagtt cactttggcc tagatttatg aaaaatgcat gcctcgtaaa gagtccactg
     4021 tattcacgag taaagttgct tttagtgttc acttgatgac ttggagagta ggaattttgc
     4081 aaaatctgaa tttaaggaaa ttctttagga taaccatttc aaaaaataaa attgctatgc
     4141 aatcttgaat attttctctt ttgcctcgta aaatgaaaat gcattcacag tttctgtaaa
     4201 ttatttagca gccttaaagt ttatcaaaaa attgtccaga ttccacgtgc agcatgcttg
     4261 gccctgcatt taatttaaga aggattaata ataatgctct gaatttttcg aaagggattc
     4321 tcctaaaccc acccacttct cttgcccagg ctgcttttta aaaatatttt tttatttttt
     4381 acttattttt aaattttctc tttttattta tttttggttt tcttgttagc cacctgttat
     4441 atgggagaac gaaaattgtt atattttgaa agtacttatt acattatttt tattttagta
     4501 tcttgatgct cctgtcaaaa gggaaatgag gcttttaaaa ataaagtacc ttaattcttt
     4561 attgactttt tgccctaaat tgctaggtgt gacccagcaa tcttttagga agagatttta
     4621 cagtggtgct ttatttatat caataatcca gtatagttag gctgttcatt cctcataata
     4681 gagtacataa cagaaaagtg ggactttcac attttcatat ttaggcacgt tccaatttaa
     4741 ttccaaaaat actctgtaat tctacatcta aaaaaaccga ttccctaatt cgaatttatt
     4801 ggtaccaaag ctctctttgg ctatagacaa ttaagagttg accttttaag ttaatgtata
     4861 tgcttaaaaa cagttttagg aaaatatttg gtagacaaag agtttcaact ttaaatgttc
     4921 actatgtcat ttagtgtcca actttacgga taggttgact atctaaatag gcatttttag
     4981 tcattaaaaa aaatctagtc accaggagga tccctataac tcaaaataac ttgtttgtaa
     5041 aagaaaattt gtttacttac ccattagtaa gttcctgcat attcattata agatggcaaa
     5101 tcaaactttt ctaggatgaa gacagcttat ttttaagttg tatagtctta gttggtttag
     5161 ggtctcaatt ttaattaata aaatacttgg tttttatttg cttgtccttt tgaattcctg
     5221 ttttaataat tttaaaatga gcacaaagaa cgttgaagtt cagattaatc tcttctgaat
     5281 gatgtttttt tcctctgtga tgagttgttt ctgacttttt tccttttgta tttgtaatgt
     5341 tgattaagat gtaaaataaa aagtgtgcct gattattttt gcaaa
//
