LOCUS       NM_001318109            1681 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens galactose-3-O-sulfotransferase 1 (GAL3ST1), transcript
            variant 15, mRNA.
ACCESSION   NM_001318109 XM_011530519
VERSION     NM_001318109.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Porubsky,S., Nientiedt,M., Kriegmair,M.C., Siemoneit,J.H.,
            Sandhoff,R., Jennemann,R., Borgmann,H., Gaiser,T., Weis,C.A.,
            Erben,P., Hielscher,T. and Popovic,Z.V.
  TITLE     The prognostic value of galactosylceramide-sulfotransferase
            (Gal3ST1) in human renal cell carcinoma
  JOURNAL   Sci Rep 11 (1), 10926 (2021)
   PUBMED   34035403
  REMARK    GeneRIF: The prognostic value of
            galactosylceramide-sulfotransferase (Gal3ST1) in human renal cell
            carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Robinson,C.M., Poon,B.P.K., Kano,Y., Pluthero,F.G., Kahr,W.H.A. and
            Ohh,M.
  TITLE     A Hypoxia-Inducible HIF1-GAL3ST1-Sulfatide Axis Enhances ccRCC
            Immune Evasion via Increased Tumor Cell-Platelet Binding
  JOURNAL   Mol Cancer Res 17 (11), 2306-2317 (2019)
   PUBMED   31427440
  REMARK    GeneRIF: These results suggest that GAL3ST1 is a HIF-responsive
            gene that may contribute to clear cell renal cell carcinoma (ccRCC)
            development via promoting cancer cell evasion of immune
            surveillance.
            Erratum:[Mol Cancer Res. 2021 Apr;19(4):739. doi:
            10.1158/1541-7786.MCR-20-0986.. PMID: 33811174]
REFERENCE   3  (bases 1 to 1681)
  AUTHORS   Liu,Y., Chen,Y., Momin,A., Shaner,R., Wang,E., Bowen,N.J.,
            Matyunina,L.V., Walker,L.D., McDonald,J.F., Sullards,M.C. and
            Merrill,A.H. Jr.
  TITLE     Elevation of sulfatides in ovarian cancer: an integrated
            transcriptomic and lipidomic analysis including tissue-imaging mass
            spectrometry
  JOURNAL   Mol Cancer 9, 186 (2010)
   PUBMED   20624317
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1681)
  AUTHORS   Roeske-Nielsen,A., Buschard,K., Manson,J.E., Rastam,L. and
            Lindblad,U.
  TITLE     A variation in the cerebroside sulfotransferase gene is linked to
            exercise-modified insulin resistance and to type 2 diabetes
  JOURNAL   Exp Diabetes Res 2009, 429593 (2009)
   PUBMED   19587831
  REMARK    GeneRIF: results suggest that heterozygosity at SNP rs2267161 in
            the gene encoding the CST enzyme confers increased risk of type 2
            diabetes; females with the CC allele showed lower insulin
            resistance
REFERENCE   5  (bases 1 to 1681)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   6  (bases 1 to 1681)
  AUTHORS   Honke,K., Tsuda,M., Hirahara,Y., Ishii,A., Makita,A. and Wada,Y.
  TITLE     Molecular cloning and expression of cDNA encoding human
            3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
  JOURNAL   J Biol Chem 272 (8), 4864-4868 (1997)
   PUBMED   9030544
REFERENCE   7  (bases 1 to 1681)
  AUTHORS   Stein,C., Gieselmann,V., Kreysing,J., Schmidt,B., Pohlmann,R.,
            Waheed,A., Meyer,H.E., O'Brien,J.S. and von Figura,K.
  TITLE     Cloning and expression of human arylsulfatase A
  JOURNAL   J Biol Chem 264 (2), 1252-1259 (1989)
   PUBMED   2562955
REFERENCE   8  (bases 1 to 1681)
  AUTHORS   Siegrist,H.P., Jutzi,H., Steck,A.J., Burkart,T., Wiesmann,U. and
            Herschkowitz,N.
  TITLE     Age-dependent modulation of
            3'-phosphoadenosine-5'-phosphosulfate-galactosylceramide
            sulfotransferase by lipids extracted from the microsomal membranes
            and artificial lipid mixtures
  JOURNAL   Biochim Biophys Acta 489 (1), 58-63 (1977)
   PUBMED   911873
REFERENCE   9  (bases 1 to 1681)
  AUTHORS   Fleischer,B. and Zambrano,F.
  TITLE     Localization of cerebroside-sulfotransferase activity in the Golgi
            apparatus of rat kidney
  JOURNAL   Biochem Biophys Res Commun 52 (3), 951-958 (1973)
   PUBMED   4710574
REFERENCE   10 (bases 1 to 1681)
  AUTHORS   Farrell,D.F. and McKhann,G.M.
  TITLE     Characterization of cerebroside sulfotransferase from rat brain
  JOURNAL   J Biol Chem 246 (15), 4694-4702 (1971)
   PUBMED   5562350
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA384904.1, BP220526.1,
            D88667.1 and AW137588.1.
            
            On Dec 24, 2015 this sequence version replaced XM_011530519.1.
            
            Summary: Sulfonation, an important step in the metabolism of many
            drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed
            by sulfotransferases. This gene encodes galactosylceramide
            sulfotransferase, which catalyzes the sulfation of membrane
            glycolipids including the final step in the synthesis of sulfatide,
            a major lipid component of the myelin sheath. This gene exhibits
            elevated expression in ovarian epithelial carcinoma and the encoded
            enzyme exhibits elevated activity in renal cell carcinoma.
            Mutations in this gene may be associated with reduced insulin
            resistance. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (15) differs in the 5' UTR and
            uses an alternate translation start site compared to variant 1. The
            encoded isoform (3) has a longer and distinct N-terminus compared
            to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP220526.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                DA384904.1         1-33
            34-160              BP220526.1         29-155
            161-1664            D88667.1           288-1791
            1665-1681           AW137588.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..1681
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="galactose-3-O-sulfotransferase 1"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:HGNC:24240"
                     /db_xref="MIM:602300"
     exon            1..67
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     CDS             2..1348
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /EC_number="2.8.2.11"
                     /note="isoform 3 is encoded by transcript variant 15;
                     cerebroside (3'-phosphoadenylylsulfate:galactosylceramide
                     3') sulfotransferase;
                     3'-phosphoadenosine-5'-phosphosulfate:GalCer
                     sulfotransferase; galactosylceramide sulfotransferase"
                     /codon_start=1
                     /product="galactosylceramide sulfotransferase isoform 3"
                     /protein_id="NP_001305038.1"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:HGNC:24240"
                     /db_xref="MIM:602300"
                     /translation="MHKWNSGPGCRLLKGSLLHFDWVSEMLPPQKKPWESMAKGLVLG
                     ALFTSFLLLVYSYAVPPLHAGLASTTPEAAASCSPPALEPEAVIRANGSAGECQPRRN
                     IVFLKTHKTASSTLLNILFRFGQKHRLKFAFPNGRNDFDYPTFFARSLVQDYRPGACF
                     NIICNHMRFHYDEVRGLVPTNAIFITVLRDPARLFESSFHYFGPVVPLTWKLSAGDKL
                     TEFLQDPDRYYDPNGFNAHYLRNLLFFDLGYDNSLDPSSPQVQEHILEVERRFHLVLL
                     QEYFDESLVLLKDLLCWELEDVLYFKLNARRDSPVPRLSGELYGRATAWNMLDSHLYR
                     HFNASFWRKVEAFGRERMAREVAALRHANERMRTICIDGGHAVDAAAIQDEAMQPWQP
                     LGTKSILGYNLKKSIGQRHAQLCRRMLTPEIQYLMDLGANLWVTKLWKFIRDFLRW"
     exon            68..207
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     exon            208..1666
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1635..1640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="hexamer: AGTAAA"
     polyA_site      1666
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="major polyA site"
ORIGIN      
        1 gatgcacaaa tggaattctg ggcccggctg caggctcctg aagggctcct tgcttcactt
       61 tgactgggtg tctgagatgc tgccaccgca gaagaagccc tgggagtcca tggctaaggg
      121 gctggtgctg ggcgcgctct tcactagttt cctgctgctg gtgtactcct atgccgtgcc
      181 cccgctgcat gccggcctgg cctccacgac cccggaggcc gcagcgtcct gctctccacc
      241 tgcactcgag ccagaggcag tgatccgggc caacggctcg gcgggggagt gccagccgcg
      301 gcgcaacatc gtgttcttga agacgcacaa gacggccagc agcaccctgc tcaacatcct
      361 gttccgcttc ggccagaagc accggctcaa gttcgccttc cctaacggcc gcaatgactt
      421 cgactacccg accttcttcg cccgcagcct ggtgcaggac tatcggcccg gggcctgctt
      481 caacatcatc tgcaaccaca tgcgcttcca ctacgacgag gtgcgcggcc tggtgccgac
      541 caacgccatc ttcatcacgg tgctccgcga ccccgcccgc ttgttcgagt cctccttcca
      601 ctacttcggg ccggtggtgc ccctcacgtg gaagctctcg gccggcgaca agctgaccga
      661 gttcctgcaa gacccggatc gctactacga ccccaacggc ttcaatgccc actacctccg
      721 aaacctgctc ttcttcgacc tgggctatga caacagcctg gaccccagca gcccgcaggt
      781 gcaggagcac atcctggagg tggagcgtcg cttccacctg gtgctccttc aagagtactt
      841 cgacgagtcg ctggtgctgc tgaaggacct gctgtgctgg gagctggagg acgtgctcta
      901 cttcaagctc aacgcccgcc gcgactcgcc cgtgccgcgg ctctcggggg agctgtatgg
      961 gcgcgccacc gcctggaaca tgctggactc ccacctctac cgccacttca acgccagctt
     1021 ctggcgcaag gtggaggcct tcgggcggga gcgcatggcc cgcgaggtgg ccgccctgcg
     1081 ccatgccaac gagcgcatgc ggaccatctg catcgacggg ggccacgccg tggacgccgc
     1141 cgccatccag gacgaggcca tgcagccctg gcagccgctg ggcaccaagt ccatcctggg
     1201 ctacaacctc aagaagagca tcgggcagcg gcacgcgcag ctctgccggc gcatgctcac
     1261 gcccgagatc cagtacctga tggacctcgg cgccaacctg tgggtcacca agctctggaa
     1321 gttcattcgc gatttcctgc ggtggtgacg tcccaccgcc cagcggcttg cctgcctgct
     1381 cgctccctgc agaggggctg agcaggacgc cgctggtgct ggccgccccc agccccctcc
     1441 tggtgccacc tcagaccccg gggtgagggg gggctccctg gggggaggca gccagccaag
     1501 actgggccca tgaacacaga gagggcctaa ccgagatcag tatttaacta attataccag
     1561 tttttattaa acccctttcc ctccccgata aagaatgttc tatttctgcc tccccttaaa
     1621 ggggagacct cagaagtaaa ggaatttgat gttgtgtttt tgttaaaaaa aaaaaaaaaa
     1681 a
//
