LOCUS       NM_001318108            1889 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens galactose-3-O-sulfotransferase 1 (GAL3ST1), transcript
            variant 5, mRNA.
ACCESSION   NM_001318108 XM_011530532
VERSION     NM_001318108.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Porubsky,S., Nientiedt,M., Kriegmair,M.C., Siemoneit,J.H.,
            Sandhoff,R., Jennemann,R., Borgmann,H., Gaiser,T., Weis,C.A.,
            Erben,P., Hielscher,T. and Popovic,Z.V.
  TITLE     The prognostic value of galactosylceramide-sulfotransferase
            (Gal3ST1) in human renal cell carcinoma
  JOURNAL   Sci Rep 11 (1), 10926 (2021)
   PUBMED   34035403
  REMARK    GeneRIF: The prognostic value of
            galactosylceramide-sulfotransferase (Gal3ST1) in human renal cell
            carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Robinson,C.M., Poon,B.P.K., Kano,Y., Pluthero,F.G., Kahr,W.H.A. and
            Ohh,M.
  TITLE     A Hypoxia-Inducible HIF1-GAL3ST1-Sulfatide Axis Enhances ccRCC
            Immune Evasion via Increased Tumor Cell-Platelet Binding
  JOURNAL   Mol Cancer Res 17 (11), 2306-2317 (2019)
   PUBMED   31427440
  REMARK    GeneRIF: These results suggest that GAL3ST1 is a HIF-responsive
            gene that may contribute to clear cell renal cell carcinoma (ccRCC)
            development via promoting cancer cell evasion of immune
            surveillance.
            Erratum:[Mol Cancer Res. 2021 Apr;19(4):739. doi:
            10.1158/1541-7786.MCR-20-0986.. PMID: 33811174]
REFERENCE   3  (bases 1 to 1889)
  AUTHORS   Liu,Y., Chen,Y., Momin,A., Shaner,R., Wang,E., Bowen,N.J.,
            Matyunina,L.V., Walker,L.D., McDonald,J.F., Sullards,M.C. and
            Merrill,A.H. Jr.
  TITLE     Elevation of sulfatides in ovarian cancer: an integrated
            transcriptomic and lipidomic analysis including tissue-imaging mass
            spectrometry
  JOURNAL   Mol Cancer 9, 186 (2010)
   PUBMED   20624317
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1889)
  AUTHORS   Roeske-Nielsen,A., Buschard,K., Manson,J.E., Rastam,L. and
            Lindblad,U.
  TITLE     A variation in the cerebroside sulfotransferase gene is linked to
            exercise-modified insulin resistance and to type 2 diabetes
  JOURNAL   Exp Diabetes Res 2009, 429593 (2009)
   PUBMED   19587831
  REMARK    GeneRIF: results suggest that heterozygosity at SNP rs2267161 in
            the gene encoding the CST enzyme confers increased risk of type 2
            diabetes; females with the CC allele showed lower insulin
            resistance
REFERENCE   5  (bases 1 to 1889)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   6  (bases 1 to 1889)
  AUTHORS   Honke,K., Tsuda,M., Hirahara,Y., Ishii,A., Makita,A. and Wada,Y.
  TITLE     Molecular cloning and expression of cDNA encoding human
            3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
  JOURNAL   J Biol Chem 272 (8), 4864-4868 (1997)
   PUBMED   9030544
REFERENCE   7  (bases 1 to 1889)
  AUTHORS   Stein,C., Gieselmann,V., Kreysing,J., Schmidt,B., Pohlmann,R.,
            Waheed,A., Meyer,H.E., O'Brien,J.S. and von Figura,K.
  TITLE     Cloning and expression of human arylsulfatase A
  JOURNAL   J Biol Chem 264 (2), 1252-1259 (1989)
   PUBMED   2562955
REFERENCE   8  (bases 1 to 1889)
  AUTHORS   Siegrist,H.P., Jutzi,H., Steck,A.J., Burkart,T., Wiesmann,U. and
            Herschkowitz,N.
  TITLE     Age-dependent modulation of
            3'-phosphoadenosine-5'-phosphosulfate-galactosylceramide
            sulfotransferase by lipids extracted from the microsomal membranes
            and artificial lipid mixtures
  JOURNAL   Biochim Biophys Acta 489 (1), 58-63 (1977)
   PUBMED   911873
REFERENCE   9  (bases 1 to 1889)
  AUTHORS   Fleischer,B. and Zambrano,F.
  TITLE     Localization of cerebroside-sulfotransferase activity in the Golgi
            apparatus of rat kidney
  JOURNAL   Biochem Biophys Res Commun 52 (3), 951-958 (1973)
   PUBMED   4710574
REFERENCE   10 (bases 1 to 1889)
  AUTHORS   Farrell,D.F. and McKhann,G.M.
  TITLE     Characterization of cerebroside sulfotransferase from rat brain
  JOURNAL   J Biol Chem 246 (15), 4694-4702 (1971)
   PUBMED   5562350
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA384904.1, DA376354.1,
            D88667.1 and AW137588.1.
            
            On May 31, 2019 this sequence version replaced NM_001318108.1.
            
            Summary: Sulfonation, an important step in the metabolism of many
            drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed
            by sulfotransferases. This gene encodes galactosylceramide
            sulfotransferase, which catalyzes the sulfation of membrane
            glycolipids including the final step in the synthesis of sulfatide,
            a major lipid component of the myelin sheath. This gene exhibits
            elevated expression in ovarian epithelial carcinoma and the encoded
            enzyme exhibits elevated activity in renal cell carcinoma.
            Mutations in this gene may be associated with reduced insulin
            resistance. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (5) differs in the 5' UTR compared
            to variant 1. Variants 1-12 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA376354.1, DA371850.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2153733, SAMN03465405
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                DA384904.1         4-35
            33-589              DA376354.1         1-557
            590-1887            D88667.1           494-1791
            1888-1889           AW137588.1         16-17               c
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..1889
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="galactose-3-O-sulfotransferase 1"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:HGNC:24240"
                     /db_xref="MIM:602300"
     exon            1..180
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     exon            181..290
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     exon            291..430
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     CDS             300..1571
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /EC_number="2.8.2.11"
                     /note="isoform 1 is encoded by transcript variant 5;
                     cerebroside (3'-phosphoadenylylsulfate:galactosylceramide
                     3') sulfotransferase;
                     3'-phosphoadenosine-5'-phosphosulfate:GalCer
                     sulfotransferase; galactosylceramide sulfotransferase"
                     /codon_start=1
                     /product="galactosylceramide sulfotransferase isoform 1"
                     /protein_id="NP_001305037.1"
                     /db_xref="CCDS:CCDS13879.1"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:HGNC:24240"
                     /db_xref="MIM:602300"
                     /translation="MLPPQKKPWESMAKGLVLGALFTSFLLLVYSYAVPPLHAGLAST
                     TPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKH
                     RLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFI
                     TVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLL
                     FFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYF
                     KLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAAL
                     RHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRR
                     MLTPEIQYLMDLGANLWVTKLWKFIRDFLRW"
     exon            431..1889
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1858..1863
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="hexamer: AGTAAA"
     polyA_site      1889
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="major polyA site"
ORIGIN      
        1 gcacaaatgg aattctgggc ccggctgcag gctcctgaag ggctccttgc ttcactttga
       61 ctgggtgagg aatatggatg agccctttta ggggctccac acgactccaa acagggcaga
      121 gcaggatgga acacaggtgc ccatgggggt catcaggctg gggactgctg gaatctccag
      181 ggtctcactg tgtcatccag agctggagtg cagcggcaca gtcatggctc actggaactc
      241 aggctcaagc aatcctcccg cctcagcctt ccaagtaact aggactacag gtgtctgaga
      301 tgctgccacc gcagaagaag ccctgggagt ccatggctaa ggggctggtg ctgggcgcgc
      361 tcttcactag tttcctgctg ctggtgtact cctatgccgt gcccccgctg catgccggcc
      421 tggcctccac gaccccggag gccgcagcgt cctgctctcc acctgcactc gagccagagg
      481 cagtgatccg ggccaacggc tcggcggggg agtgccagcc gcggcgcaac atcgtgttct
      541 tgaagacgca caagacggcc agcagcaccc tgctcaacat cctgttccgc ttcggccaga
      601 agcaccggct caagttcgcc ttccctaacg gccgcaatga cttcgactac ccgaccttct
      661 tcgcccgcag cctggtgcag gactatcggc ccggggcctg cttcaacatc atctgcaacc
      721 acatgcgctt ccactacgac gaggtgcgcg gcctggtgcc gaccaacgcc atcttcatca
      781 cggtgctccg cgaccccgcc cgcttgttcg agtcctcctt ccactacttc gggccggtgg
      841 tgcccctcac gtggaagctc tcggccggcg acaagctgac cgagttcctg caagacccgg
      901 atcgctacta cgaccccaac ggcttcaatg cccactacct ccgaaacctg ctcttcttcg
      961 acctgggcta tgacaacagc ctggacccca gcagcccgca ggtgcaggag cacatcctgg
     1021 aggtggagcg tcgcttccac ctggtgctcc ttcaagagta cttcgacgag tcgctggtgc
     1081 tgctgaagga cctgctgtgc tgggagctgg aggacgtgct ctacttcaag ctcaacgccc
     1141 gccgcgactc gcccgtgccg cggctctcgg gggagctgta tgggcgcgcc accgcctgga
     1201 acatgctgga ctcccacctc taccgccact tcaacgccag cttctggcgc aaggtggagg
     1261 ccttcgggcg ggagcgcatg gcccgcgagg tggccgccct gcgccatgcc aacgagcgca
     1321 tgcggaccat ctgcatcgac gggggccacg ccgtggacgc cgccgccatc caggacgagg
     1381 ccatgcagcc ctggcagccg ctgggcacca agtccatcct gggctacaac ctcaagaaga
     1441 gcatcgggca gcggcacgcg cagctctgcc ggcgcatgct cacgcccgag atccagtacc
     1501 tgatggacct cggcgccaac ctgtgggtca ccaagctctg gaagttcatt cgcgatttcc
     1561 tgcggtggtg acgtcccacc gcccagcggc ttgcctgcct gctcgctccc tgcagagggg
     1621 ctgagcagga cgccgctggt gctggccgcc cccagccccc tcctggtgcc acctcagacc
     1681 ccggggtgag ggggggctcc ctggggggag gcagccagcc aagactgggc ccatgaacac
     1741 agagagggcc taaccgagat cagtatttaa ctaattatac cagtttttat taaacccctt
     1801 tccctccccg ataaagaatg ttctatttct gcctcccctt aaaggggaga cctcagaagt
     1861 aaaggaattt gatgttgtgt ttttgttaa
//
