LOCUS       NM_001318104            1909 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens galactose-3-O-sulfotransferase 1 (GAL3ST1), transcript
            variant 3, mRNA.
ACCESSION   NM_001318104 XM_011530533
VERSION     NM_001318104.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Porubsky,S., Nientiedt,M., Kriegmair,M.C., Siemoneit,J.H.,
            Sandhoff,R., Jennemann,R., Borgmann,H., Gaiser,T., Weis,C.A.,
            Erben,P., Hielscher,T. and Popovic,Z.V.
  TITLE     The prognostic value of galactosylceramide-sulfotransferase
            (Gal3ST1) in human renal cell carcinoma
  JOURNAL   Sci Rep 11 (1), 10926 (2021)
   PUBMED   34035403
  REMARK    GeneRIF: The prognostic value of
            galactosylceramide-sulfotransferase (Gal3ST1) in human renal cell
            carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Robinson,C.M., Poon,B.P.K., Kano,Y., Pluthero,F.G., Kahr,W.H.A. and
            Ohh,M.
  TITLE     A Hypoxia-Inducible HIF1-GAL3ST1-Sulfatide Axis Enhances ccRCC
            Immune Evasion via Increased Tumor Cell-Platelet Binding
  JOURNAL   Mol Cancer Res 17 (11), 2306-2317 (2019)
   PUBMED   31427440
  REMARK    GeneRIF: These results suggest that GAL3ST1 is a HIF-responsive
            gene that may contribute to clear cell renal cell carcinoma (ccRCC)
            development via promoting cancer cell evasion of immune
            surveillance.
            Erratum:[Mol Cancer Res. 2021 Apr;19(4):739. doi:
            10.1158/1541-7786.MCR-20-0986.. PMID: 33811174]
REFERENCE   3  (bases 1 to 1909)
  AUTHORS   Liu,Y., Chen,Y., Momin,A., Shaner,R., Wang,E., Bowen,N.J.,
            Matyunina,L.V., Walker,L.D., McDonald,J.F., Sullards,M.C. and
            Merrill,A.H. Jr.
  TITLE     Elevation of sulfatides in ovarian cancer: an integrated
            transcriptomic and lipidomic analysis including tissue-imaging mass
            spectrometry
  JOURNAL   Mol Cancer 9, 186 (2010)
   PUBMED   20624317
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1909)
  AUTHORS   Roeske-Nielsen,A., Buschard,K., Manson,J.E., Rastam,L. and
            Lindblad,U.
  TITLE     A variation in the cerebroside sulfotransferase gene is linked to
            exercise-modified insulin resistance and to type 2 diabetes
  JOURNAL   Exp Diabetes Res 2009, 429593 (2009)
   PUBMED   19587831
  REMARK    GeneRIF: results suggest that heterozygosity at SNP rs2267161 in
            the gene encoding the CST enzyme confers increased risk of type 2
            diabetes; females with the CC allele showed lower insulin
            resistance
REFERENCE   5  (bases 1 to 1909)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   6  (bases 1 to 1909)
  AUTHORS   Honke,K., Tsuda,M., Hirahara,Y., Ishii,A., Makita,A. and Wada,Y.
  TITLE     Molecular cloning and expression of cDNA encoding human
            3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
  JOURNAL   J Biol Chem 272 (8), 4864-4868 (1997)
   PUBMED   9030544
REFERENCE   7  (bases 1 to 1909)
  AUTHORS   Stein,C., Gieselmann,V., Kreysing,J., Schmidt,B., Pohlmann,R.,
            Waheed,A., Meyer,H.E., O'Brien,J.S. and von Figura,K.
  TITLE     Cloning and expression of human arylsulfatase A
  JOURNAL   J Biol Chem 264 (2), 1252-1259 (1989)
   PUBMED   2562955
REFERENCE   8  (bases 1 to 1909)
  AUTHORS   Siegrist,H.P., Jutzi,H., Steck,A.J., Burkart,T., Wiesmann,U. and
            Herschkowitz,N.
  TITLE     Age-dependent modulation of
            3'-phosphoadenosine-5'-phosphosulfate-galactosylceramide
            sulfotransferase by lipids extracted from the microsomal membranes
            and artificial lipid mixtures
  JOURNAL   Biochim Biophys Acta 489 (1), 58-63 (1977)
   PUBMED   911873
REFERENCE   9  (bases 1 to 1909)
  AUTHORS   Fleischer,B. and Zambrano,F.
  TITLE     Localization of cerebroside-sulfotransferase activity in the Golgi
            apparatus of rat kidney
  JOURNAL   Biochem Biophys Res Commun 52 (3), 951-958 (1973)
   PUBMED   4710574
REFERENCE   10 (bases 1 to 1909)
  AUTHORS   Farrell,D.F. and McKhann,G.M.
  TITLE     Characterization of cerebroside sulfotransferase from rat brain
  JOURNAL   J Biol Chem 246 (15), 4694-4702 (1971)
   PUBMED   5562350
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005006.2, BM923857.1,
            DA271385.1, D88667.1 and AW137588.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001318104.1.
            
            Summary: Sulfonation, an important step in the metabolism of many
            drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed
            by sulfotransferases. This gene encodes galactosylceramide
            sulfotransferase, which catalyzes the sulfation of membrane
            glycolipids including the final step in the synthesis of sulfatide,
            a major lipid component of the myelin sheath. This gene exhibits
            elevated expression in ovarian epithelial carcinoma and the encoded
            enzyme exhibits elevated activity in renal cell carcinoma.
            Mutations in this gene may be associated with reduced insulin
            resistance. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1-12 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM923857.1, DA271385.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2159080 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000406361.6/ ENSP00000385207.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AC005006.2         23870-23947         c
            79-132              BM923857.1         1-54
            133-685             DA271385.1         1-553
            686-1907            D88667.1           570-1791
            1908-1909           AW137588.1         16-17               c
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..1909
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="galactose-3-O-sulfotransferase 1"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:HGNC:24240"
                     /db_xref="MIM:602300"
     exon            1..200
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     exon            201..310
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     exon            311..450
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..1591
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /EC_number="2.8.2.11"
                     /note="isoform 1 is encoded by transcript variant 3;
                     cerebroside (3'-phosphoadenylylsulfate:galactosylceramide
                     3') sulfotransferase;
                     3'-phosphoadenosine-5'-phosphosulfate:GalCer
                     sulfotransferase; galactosylceramide sulfotransferase"
                     /codon_start=1
                     /product="galactosylceramide sulfotransferase isoform 1"
                     /protein_id="NP_001305033.1"
                     /db_xref="CCDS:CCDS13879.1"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:HGNC:24240"
                     /db_xref="MIM:602300"
                     /translation="MLPPQKKPWESMAKGLVLGALFTSFLLLVYSYAVPPLHAGLAST
                     TPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKH
                     RLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFI
                     TVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLL
                     FFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYF
                     KLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAAL
                     RHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRR
                     MLTPEIQYLMDLGANLWVTKLWKFIRDFLRW"
     exon            451..1909
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1878..1883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="hexamer: AGTAAA"
     polyA_site      1909
                     /gene="GAL3ST1"
                     /gene_synonym="CST"
                     /note="major polyA site"
ORIGIN      
        1 aggctggcgg cggcacctgg ccgggagcgg gcgcggcgcg ggagcgggcg cggcgcggca
       61 gcggcggcgg gggcgggggc atggaccagg cctggtggag cggggggccc ggccgggtgc
      121 gccccgccgc ccagtgagcg cccgggcggc gcggcccggg ccgagccagc cacgctgagg
      181 cggaggagga cggggacaag ggtctcactg tgtcatccag agctggagtg cagcggcaca
      241 gtcatggctc actggaactc aggctcaagc aatcctcccg cctcagcctt ccaagtaact
      301 aggactacag gtgtctgaga tgctgccacc gcagaagaag ccctgggagt ccatggctaa
      361 ggggctggtg ctgggcgcgc tcttcactag tttcctgctg ctggtgtact cctatgccgt
      421 gcccccgctg catgccggcc tggcctccac gaccccggag gccgcagcgt cctgctctcc
      481 acctgcactc gagccagagg cagtgatccg ggccaacggc tcggcggggg agtgccagcc
      541 gcggcgcaac atcgtgttct tgaagacgca caagacggcc agcagcaccc tgctcaacat
      601 cctgttccgc ttcggccaga agcaccggct caagttcgcc ttccctaacg gccgcaatga
      661 cttcgactac ccgaccttct tcgcccgcag cctggtgcag gactatcggc ccggggcctg
      721 cttcaacatc atctgcaacc acatgcgctt ccactacgac gaggtgcgcg gcctggtgcc
      781 gaccaacgcc atcttcatca cggtgctccg cgaccccgcc cgcttgttcg agtcctcctt
      841 ccactacttc gggccggtgg tgcccctcac gtggaagctc tcggccggcg acaagctgac
      901 cgagttcctg caagacccgg atcgctacta cgaccccaac ggcttcaatg cccactacct
      961 ccgaaacctg ctcttcttcg acctgggcta tgacaacagc ctggacccca gcagcccgca
     1021 ggtgcaggag cacatcctgg aggtggagcg tcgcttccac ctggtgctcc ttcaagagta
     1081 cttcgacgag tcgctggtgc tgctgaagga cctgctgtgc tgggagctgg aggacgtgct
     1141 ctacttcaag ctcaacgccc gccgcgactc gcccgtgccg cggctctcgg gggagctgta
     1201 tgggcgcgcc accgcctgga acatgctgga ctcccacctc taccgccact tcaacgccag
     1261 cttctggcgc aaggtggagg ccttcgggcg ggagcgcatg gcccgcgagg tggccgccct
     1321 gcgccatgcc aacgagcgca tgcggaccat ctgcatcgac gggggccacg ccgtggacgc
     1381 cgccgccatc caggacgagg ccatgcagcc ctggcagccg ctgggcacca agtccatcct
     1441 gggctacaac ctcaagaaga gcatcgggca gcggcacgcg cagctctgcc ggcgcatgct
     1501 cacgcccgag atccagtacc tgatggacct cggcgccaac ctgtgggtca ccaagctctg
     1561 gaagttcatt cgcgatttcc tgcggtggtg acgtcccacc gcccagcggc ttgcctgcct
     1621 gctcgctccc tgcagagggg ctgagcagga cgccgctggt gctggccgcc cccagccccc
     1681 tcctggtgcc acctcagacc ccggggtgag ggggggctcc ctggggggag gcagccagcc
     1741 aagactgggc ccatgaacac agagagggcc taaccgagat cagtatttaa ctaattatac
     1801 cagtttttat taaacccctt tccctccccg ataaagaatg ttctatttct gcctcccctt
     1861 aaaggggaga cctcagaagt aaaggaattt gatgttgtgt ttttgttaa
//
