LOCUS       NM_001371727            7266 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens gamma-aminobutyric acid type A receptor subunit beta2
            (GABRB2), transcript variant 3, mRNA.
ACCESSION   NM_001371727
VERSION     NM_001371727.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Li,P.P., Zhou,Y.Y., Gao,L., Lv,J.N., Xu,S.S., Zhao,Y.W., Xu,D.,
            Huang,R., Zhang,X., Li,P., Fu,X. and He,Z.
  TITLE     The de novo missense mutation F224S in GABRB2, identified in
            epileptic encephalopathy and developmental delay, impairs GABAAR
            function
  JOURNAL   Neuroscience 553, 172-184 (2024)
   PUBMED   38964454
  REMARK    GeneRIF: The de novo missense mutation F224S in GABRB2, identified
            in epileptic encephalopathy and developmental delay, impairs GABAAR
            function.
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Mohammadi,N.A., Ahring,P.K., Yu Liao,V.W., Chua,H.C., Ortiz de la
            Rosa,S., Johannesen,K.M., Michaeli-Yossef,Y., Vincent-Devulder,A.,
            Meridda,C., Bruel,A.L., Rossi,A., Patel,C., Klepper,J., Bonanni,P.,
            Minghetti,S., Trivisano,M., Specchio,N., Amor,D., Auvin,S.,
            Baer,S., Meyer,P., Milh,M., Salpietro,V., Maroofian,R., Lemke,J.R.,
            Weckhuysen,S., Christophersen,P., Rubboli,G., Chebib,M.,
            Jensen,A.A., Absalom,N.L. and Moller,R.S.
  TITLE     Distinct neurodevelopmental and epileptic phenotypes associated
            with gain- and loss-of-function GABRB2 variants
  JOURNAL   EBioMedicine 106, 105236 (2024)
   PUBMED   38996765
  REMARK    GeneRIF: Distinct neurodevelopmental and epileptic phenotypes
            associated with gain- and loss-of-function GABRB2 variants.
REFERENCE   3  (bases 1 to 7266)
  AUTHORS   Sente,A., Desai,R., Naydenova,K., Malinauskas,T., Jounaidi,Y.,
            Miehling,J., Zhou,X., Masiulis,S., Hardwick,S.W., Chirgadze,D.Y.,
            Miller,K.W. and Aricescu,A.R.
  TITLE     Differential assembly diversifies GABAA receptor structures and
            signalling
  JOURNAL   Nature 604 (7904), 190-194 (2022)
   PUBMED   35355020
REFERENCE   4  (bases 1 to 7266)
  AUTHORS   Barki,M. and Xue,H.
  TITLE     GABRB2, a key player in neuropsychiatric disorders and beyond
  JOURNAL   Gene 809, 146021 (2022)
   PUBMED   34673206
  REMARK    GeneRIF: GABRB2, a key player in neuropsychiatric disorders and
            beyond.
            Review article
REFERENCE   5  (bases 1 to 7266)
  AUTHORS   El Achkar,C.M., Harrer,M., Smith,L., Kelly,M., Iqbal,S.,
            Maljevic,S., Niturad,C.E., Vissers,L.E.L.M., Poduri,A., Yang,E.,
            Lal,D., Lerche,H., Moller,R.S. and Olson,H.E.
  CONSRTM   GABRB2 Working Group
  TITLE     Characterization of the GABRB2-Associated Neurodevelopmental
            Disorders
  JOURNAL   Ann Neurol 89 (3), 573-586 (2021)
   PUBMED   33325057
  REMARK    GeneRIF: Characterization of the GABRB2-Associated
            Neurodevelopmental Disorders.
            Erratum:[Ann Neurol. 2021 Nov;90(5):859. doi: 10.1002/ana.26215..
            PMID: 34625987]
REFERENCE   6  (bases 1 to 7266)
  AUTHORS   McKinley,D.D., Lennon,D.J. and Carter,D.B.
  TITLE     Cloning, sequence analysis and expression of two forms of mRNA
            coding for the human beta 2 subunit of the GABAA receptor
  JOURNAL   Brain Res Mol Brain Res 28 (1), 175-179 (1995)
   PUBMED   7707873
REFERENCE   7  (bases 1 to 7266)
  AUTHORS   Russek,S.J. and Farb,D.H.
  TITLE     Mapping of the beta 2 subunit gene (GABRB2) to microdissected human
            chromosome 5q34-q35 defines a gene cluster for the most abundant
            GABAA receptor isoform
  JOURNAL   Genomics 23 (3), 528-533 (1994)
   PUBMED   7851879
REFERENCE   8  (bases 1 to 7266)
  AUTHORS   Togel,M., Mossier,B., Fuchs,K. and Sieghart,W.
  TITLE     gamma-Aminobutyric acidA receptors displaying association of gamma
            3-subunits with beta 2/3 and different alpha-subunits exhibit
            unique pharmacological properties
  JOURNAL   J Biol Chem 269 (17), 12993-12998 (1994)
   PUBMED   8175718
REFERENCE   9  (bases 1 to 7266)
  AUTHORS   Kellenberger,S., Malherbe,P. and Sigel,E.
  TITLE     Function of the alpha 1 beta 2 gamma 2S gamma-aminobutyric acid
            type A receptor is modulated by protein kinase C via multiple
            phosphorylation sites
  JOURNAL   J Biol Chem 267 (36), 25660-25663 (1992)
   PUBMED   1334482
REFERENCE   10 (bases 1 to 7266)
  AUTHORS   Moss,S.J., Doherty,C.A. and Huganir,R.L.
  TITLE     Identification of the cAMP-dependent protein kinase and protein
            kinase C phosphorylation sites within the major intracellular
            domains of the beta 1, gamma 2S, and gamma 2L subunits of the
            gamma-aminobutyric acid type A receptor
  JOURNAL   J Biol Chem 267 (20), 14470-14476 (1992)
   PUBMED   1321150
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091989.3, AC091882.3,
            AC008712.8 and AC022120.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The gamma-aminobutyric acid (GABA) A receptor is a
            multisubunit chloride channel that mediates the fastest inhibitory
            synaptic transmission in the central nervous system. This gene
            encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome
            5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2
            subunits of the GABA A receptor. Alternative splicing of this gene
            generates 2 transcript variants, differing by a 114 bp insertion.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.10347.1,
                                           SRR1660809.145594.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2157437 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393959.6/ ENSP00000377531.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               AC091989.3         78835-78993
            160-251             AC091989.3         79147-79238
            252-319             AC091989.3         80266-80333
            320-540             AC091882.3         123420-123640       c
            541-623             AC008712.8         134588-134670       c
            624-761             AC022120.7         3377-3514
            762-914             AC022120.7         5242-5394
            915-1159            AC022120.7         9019-9263
            1160-1273           AC022120.7         13665-13778
            1274-7266           AC022120.7         45718-51710
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q34"
     gene            1..7266
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="gamma-aminobutyric acid type A receptor subunit
                     beta2"
                     /db_xref="GeneID:2561"
                     /db_xref="HGNC:HGNC:4082"
                     /db_xref="MIM:600232"
     exon            1..159
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..1621
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 3; gamma-aminobutyric acid A receptor beta 2;
                     GABA(A) receptor subunit beta-2; gamma-aminobutyric acid
                     type A receptor beta2 subunit; GABAAR subunit beta-2"
                     /codon_start=1
                     /product="gamma-aminobutyric acid receptor subunit beta-2
                     isoform 1 precursor"
                     /protein_id="NP_001358656.1"
                     /db_xref="CCDS:CCDS4355.1"
                     /db_xref="GeneID:2561"
                     /db_xref="HGNC:HGNC:4082"
                     /db_xref="MIM:600232"
                     /translation="MWRVRKRGYFGIWSFPLIIAAVCAQSVNDPSNMSLVKETVDRLL
                     KGYDIRLRPDFGGPPVAVGMNIDIASIDMVSEVNMDYTLTMYFQQAWRDKRLSYNVIP
                     LNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAAC
                     MMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGDDNAVTGVTKIELPQFSIVDYKLI
                     TKKVVFSTGSYPRLSLSFKLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVA
                     LGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFMALLEYALVNYIFFGRG
                     PQRQKKAAEKAASANNEKMRLDVNKIFYKDIKQNGTQYRSLWDPTGNLSPTRRTTNYD
                     FSLYTMDPHENILLSTLEIKNEMATSEAVMGLGDPRSTMLAYDASSIQYRKAGLPRHS
                     FGRNALERHVAQKKSRLRRRASQLKITIPDLTDVNAIDRWSRIFFPVVFSFFNIVYWL
                     YYVN"
     exon            160..251
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            252..319
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            320..540
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            541..623
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            624..761
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            762..914
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1159
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1273
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..7266
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5389..5394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="hexamer: AACAAA"
     polyA_site      5409
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="major polyA site"
     regulatory      5691..5696
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="hexamer: ATTAAA"
     polyA_site      5711
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
     regulatory      7253..7258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="hexamer: AATAAA"
     polyA_site      7266
                     /gene="GABRB2"
                     /gene_synonym="DEE92; ICEE2"
                     /note="major polyA site"
ORIGIN      
        1 acattagaat tactgcactg ggcagactaa gttggatctc ctctcttcag tgaaaccctc
       61 aattccatca aaaactaaag ggatgtggag agtgcggaaa aggggctact ttgggatttg
      121 gtccttcccc ttaataatcg ccgctgtctg tgcgcagagt gtcaatgacc ctagtaatat
      181 gtcgctggtt aaagagacgg tggatagact cctgaaaggc tatgacattc gtctgagacc
      241 agattttgga ggtccccccg tggctgtggg gatgaacatt gacattgcca gcatcgatat
      301 ggtttctgaa gtcaatatgg attatacctt gacaatgtac tttcaacaag cctggagaga
      361 taagaggctg tcctataatg taataccttt aaacttgact ctggacaaca gagtggcaga
      421 ccagctctgg gtgcctgata cctatttcct gaacgataag aagtcatttg tgcacggagt
      481 gactgttaag aaccgcatga ttcgcctgca tcctgatggc accgtccttt atggactcag
      541 aatcacaacc acagctgcct gcatgatgga cctaaggagg tacccactgg atgaacaaaa
      601 ctgcaccttg gaaattgaga gctatggata cacaactgat gacattgagt tttactggcg
      661 tggcgatgat aatgcagtaa caggagtaac gaaaattgaa cttccacagt tctctattgt
      721 agattacaaa cttatcacca agaaggttgt tttttccaca ggttcctatc ccaggttatc
      781 cctcagcttt aagcttaaga gaaacattgg ctactttatc ctgcaaacat acatgccttc
      841 catcctgatt accatcctct cctgggtctc cttctggatt aattacgatg cttcagctgc
      901 aagggtggca ttaggaatca caactgtcct cacaatgacc acaatcaaca cccacctccg
      961 ggaaactctc cctaaaatcc cctatgtgaa ggccattgac atgtacctga tggggtgctt
     1021 tgtcttcgtt ttcatggccc ttctggaata tgccctagtc aactacatct tctttgggag
     1081 ggggccccaa cgccaaaaga aagcagctga gaaggctgcc agtgccaaca atgagaagat
     1141 gcgcctggat gtcaacaaga ttttttataa agatattaaa caaaatggga cccaatatcg
     1201 atccttgtgg gaccctactg gaaacctctc cccaactaga cggactacca attacgattt
     1261 ctctctgtat acgatggacc cccatgagaa catcttactg agcactctcg agataaaaaa
     1321 tgaaatggcc acatctgagg ctgtgatggg acttggagac cccagaagca caatgctagc
     1381 ctatgatgcc tccagcatcc agtatcggaa agctgggttg cccaggcata gttttggccg
     1441 aaatgctctg gaacgacatg tggcgcaaaa gaaaagtcgc ctgaggagac gcgcctccca
     1501 actgaaaatc accatccctg acttgactga tgtgaatgcc atagatcggt ggtcccgcat
     1561 attcttccca gtggtttttt ccttcttcaa catcgtctat tggctttatt atgtgaacta
     1621 aaacatggcc tcccactgga agcaaggact agattcctcc tcaaaccagt tgtacagcct
     1681 gatgtaggac ttggaaaaca catcaatcca ggacaaaagt gacgctaaaa taccttagtt
     1741 gctggcctat cctgtggtcc atttcatacc atttgggttg cttctgctaa gtaatgaata
     1801 cactaaggtc cttgtggttt tccagttaaa acgcaagtga tttttacaca tggtggctag
     1861 acagcctgag tgctccatgt tgtctgttta gtatgcagcc tactcagagg ggaataattt
     1921 ggaagatatt cttagaaggc tcaacagcat tgtcagtcat ttggtcataa ccagatactc
     1981 ctcatcttcc tttttaaggt cggcatgctg ttgagatggc attgtcctta tgaaacatta
     2041 tctgtaatgc catgcaaaca cattacttga agatgatcta tgattttctt actgagatga
     2101 taatggtgtc atgggaagat tattcccact ggtttctaaa agattctctt gtgatttgag
     2161 caggctaggg gaatcaagac agagactcag gaaaccgatt tggccagtcc ctttgcttga
     2221 gcacggagaa tcaactatga tgtctttcat tttggcattt agagatgagg agacttctgg
     2281 acaatatcat gcatggtggc caagaaaatt tttgctaaac ggctatttcc taggtggtta
     2341 aagtaagagt taacaacaca gagcagagta gaaaatcaga aacgacggtt gtaacaaaaa
     2401 gcaagcaagc aagaaagcat gacccttatc agctccacac agcatctggt ctatacatac
     2461 tgtgtctagt ggcagagggt gagtaagaga gattgttttt ccaacatcac tagcttttga
     2521 cagcatacct aaagaggttt ttatagttta atcgaagata accatgaaat acaaaaatta
     2581 gagaaatgag aattgtctta gatattcagg ttagattcag agtcattcaa atatacaaat
     2641 acagagcaca aactagcaaa cagattgttt ttctcagacg aagtcagctg tccctgcaat
     2701 ttttgtccct ataattttat ttcttaattt aatagagtat ttcaagagaa ggatttgttc
     2761 atcactttaa aggattcatg gtcctctcca gctttttttt tgcttccaaa attctgatta
     2821 tattcttagg agactggcaa agtaaggctt tggagagatt gagtgaacta tattttctta
     2881 tatgcaatga aattcaccat tgtatttttc aaagcaaata atacttcaag gagtatgaac
     2941 acacagagag ttaatttgtg cattcattgg tttggggctt gattttctta agagtgcttt
     3001 cattgtatat gttgcaatct gcctaatcaa ggaaaatatg aaaactagtg gaaaataata
     3061 acaaagccac attaacatga taaatgttat gagcctcagt tgatattgca gaaaaatata
     3121 taactgttaa tcaggcaggc atgatgtatt ttttttctat ttttaagtta tgccattgtg
     3181 cttctttcta cactaattgg acataagcat tcatatgttg gacaagagga gaataagcta
     3241 atatgtcttc cttctatcaa tataaggctc atattttgct tttcttatag aaaatagtga
     3301 attgctaaat tttccttaaa gttgaatttg attgaacttt ttcttggagg atgactttgc
     3361 tatttctatt ttgaaaacaa agaaagctct tctttttaga gtaatatccc taattgtgca
     3421 tagcagccag tatccacctg tgaataatgt atgttttctt gattccttgt ttccttcatg
     3481 atttagcagc ttggaatatg atggagtact attgcccttg cattccagct gtccaagaac
     3541 aacaacaaac ctttatcctt cagctttctg aatttgcccc aaaatggaat ggtattttgt
     3601 ttttcaccag tgtaggaaac cttggccatt atattttgat tacataatct tgagcacctc
     3661 tgagtatcat ctttgggcag aatctgacca agattcttct cacaggctca gagtagagat
     3721 tcctcatgtt tctccaggac acactttctc ctaggcaatc attttggggc atcacactaa
     3781 gtaaagtgcc tgccccttgt caacaggggg taaagtttga ctaaaaaaca tcaggaagga
     3841 acatattgat aactcagggg cacaactcca aaatgccatt ctgaaaaggc tcttctagag
     3901 tcaactcaga gcccaagtgt agaagtgggc tcttatgtgc aatcactgta caagccataa
     3961 aaatctttga tattgccatt tatgattaca acaaaatttt agatgtccaa gaaagtcaac
     4021 agaacaatcc agatcaaagt gtagagaagc atctttgcag cttgagcctc aaaagcataa
     4081 aaccaggaat tcatttctgc tctcagtgat acaagaaaca aggcaaagga aaaaagatgg
     4141 gaccttagaa gcaatgccct agagtaaaaa gcatacattg gcactcagta accacagaga
     4201 tagaaacaac tgactcagcc tctccagaaa taacagtaga tgtagaattt gttcttaaat
     4261 atatgtttcg ttgaaatgaa atgcagtcat caaatgcgag gaggggtgct agacctgata
     4321 agtctaggaa gaccagtcag cttgatagcc agttgttaaa ctaaaggacc acaaaggtgt
     4381 gtttagtagt tctgatatgg gattactatc agtttataca tatgtataga ttttctttta
     4441 attataaaca gccagatagc cagcaattaa ggtgtttttt catctttgtg aaattcatgg
     4501 acaaattctg actctaaaat gcacagtcta catgaaatct ttgatctcaa tctgtatata
     4561 tatatattgt acatgaccgc tagtaggtat gaaatgcatt tcagagttga aaactcatgc
     4621 aacataatca tgtgtttgca taaggaatgt taaaattctt tctactacaa tttattaaca
     4681 ggggaaaaga ttcaagcagt gccatctaga ccttttctct tctcatttct tcaaaagcag
     4741 tgctaagtaa aataataata ataaaaatgg tttatgaacc aaaagacttt acattctagc
     4801 attaggaaca cacacaaaaa aatctgtcag cttacaaaag cacaacacat aaagaaagag
     4861 cagtggaatg cgtaatgggg aaatgaatca tgtttaaagc aagacttgca ttaaattggc
     4921 aactaagata atttccattt tctataggta accgtgttcc agtttaatga ttaccttcat
     4981 actcaacaag aaaaatggca aagcaacatc atcaaaatag gtcttacaac atatctacat
     5041 gtatatatgt gtatatatag attcatataa acatacatac atatatatgc attcatatgt
     5101 tggaaaacaa aaggaataag ctaatatgtt ttatttcctt cattaaacca gtatatgagt
     5161 atttttaatt tggcaaacaa aaatcaacat actgaaataa gtaatacagg gtactgagca
     5221 tttctctatc agcaaatcaa tgcctacagt ttttatgaac cattgcacag ttcaactcga
     5281 caatagtact gtaccccaga acatcagaag ttaagtagtt gtcacattgc ttgtctaatt
     5341 gctctctttt tcaaggaaac aaataacatt gtccattggt gagtcaaaaa caaacatgtc
     5401 ttaagaaaat gaaaaatgat aaaaattcct ccaaagagga agaaaaaaaa aaaactaaac
     5461 cccaccatgt tggttagggc aaaatactaa gtattcacaa ctgtagcaat catgccattt
     5521 ggctaaatgt acagagtaca tgtaatgtga gcaaccataa ctttcactga acgtgtgtaa
     5581 atattaaaac agatttctta aatattttta acttctaatt tgtattttat ggtaaggcaa
     5641 tgtgctaatt ccgtttatgg cttaagttga tgtgttataa tatgtaaata attaaacatg
     5701 ctgtaaatga atagtcaata gatacaaata attgtttagt ttttttctta atttttattt
     5761 attttttact aagtactatt actgcagtgc ccccagcaga gtaatttaaa cacccttttg
     5821 cataaatggt ttgggaatcc cagacagaag ggtttcaatt tgctatcaca cacacagtca
     5881 cacacagtca cacacacaca cacacacaca ctcaaacaca cataatcttt tgctcaccca
     5941 ctctgtctct ctgcccgtct atgggaaaca ctctactatt tatgtagtca atataacaaa
     6001 caagagatag cctttgaaat ggcaattggg aaaagcaaaa atgatcattg ataacttaat
     6061 agactttttt atatgtcaga gttcaagaaa ccaaagtttg caacttaaat ctcagttttc
     6121 aaacagtaaa agtttgtata gatggcacta cagaaaaata aaaatggtta ttcctaccag
     6181 ctccagaaat gtgttacaca atatacacta gaagactttt tttttaataa tagtattttg
     6241 tccttaggta gtattgttcc tttcaaccaa agactccaga attattgaaa aacaaaaaag
     6301 gaaaaaaaaa atgagataat gggggaaaaa aaggacgctg cttcacacca aaggtttaaa
     6361 aaaatatttt ccaaatgcac taggaacatt gtaaaaacca gaaaggagtg aacagaatgc
     6421 taatgaagat actgccctaa agaaatctgt acaaaaaaaa aaaaaaaaag cagcactctt
     6481 aaaaagctac taggtttttg gaaattgaag taaaatattt ctgtagatat attcaaaatt
     6541 ctgacatcaa attttaattt gcttaaagaa gttttatttg tgatgaagat caatatgatt
     6601 caaatgatga aaaacaaaac tgtatgtcct ttgagttccc aaaccaaatg tcactgccgt
     6661 gtcacatcct ctaatctgat gcccatgtgt gtgtccttgc gccatttgaa gcaagttgac
     6721 agttccatca gtttgttttt gtttttgttt ttggttcaat aatttgagtt caaccatcgt
     6781 cattttaaac ataattaatc ccaacaaagt tgcctatagt ttaatttaaa cctcacagca
     6841 tttttaattt attttgcatg ggtgtttaaa tagttctgtt acgaatattg tccctgcaca
     6901 gttctaaatt gtccttttat aaaaaaaaaa agtgatattc tagtttcttc acaatgtagt
     6961 gattatcagc cttactaatt accttgtagc ctttcttaat atgcacataa tgcacatttt
     7021 ccgatggcta gaaaaatgca aaacacagtg gatatcattg catctatttt catgtctttc
     7081 taaaaacagg actattaaaa tctctgggat acatgagata gtaaaaaatg aggattataa
     7141 atgaatagca cataagaatt attttcttga atttaaactt attgcagcca gtttcagcat
     7201 gtaaatatat aataatgttg gctagtgtgt aattcttgaa ctaagaaata taaataaaaa
     7261 taaaaa
//
