LOCUS       NM_005754              10227 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens G3BP stress granule assembly factor 1 (G3BP1),
            transcript variant 1, mRNA.
ACCESSION   NM_005754
VERSION     NM_005754.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Qin,M., Fan,W., Chen,F., Ruan,K. and Liu,D.
  TITLE     Caprin1 Bridges PRMT1 to G3BP1 and Spaces Them to Ensure Proper
            Stress Granule Formation
  JOURNAL   J Mol Biol 436 (19), 168727 (2024)
   PUBMED   39079611
  REMARK    GeneRIF: Caprin1 Bridges PRMT1 to G3BP1 and Spaces Them to Ensure
            Proper Stress Granule Formation.
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   3  (bases 1 to 10227)
  AUTHORS   Yao,Z., Liu,Y., Chen,Q., Chen,X., Zhu,Z., Song,S., Ma,X. and
            Yang,P.
  TITLE     The divergent effects of G3BP orthologs on human stress granule
            assembly imply a centric role for the core protein interaction
            network
  JOURNAL   Cell Rep 43 (8), 114617 (2024)
   PUBMED   39120973
  REMARK    GeneRIF: The divergent effects of G3BP orthologs on human stress
            granule assembly imply a centric role for the core protein
            interaction network.
REFERENCE   4  (bases 1 to 10227)
  AUTHORS   Hacibeyoglu,K., Tuzlakoglu Ozturk,M., Arslan,O. and Tazebay,U.H.
  TITLE     Live Cell Protein Imaging of Tandem Complemented-GFP11-Tagged
            Coiled-Coil Domain-Containing Protein-124 Identifies this Factor in
            G3BP1-Induced Stress-Granules
  JOURNAL   Protein J 43 (4), 834-841 (2024)
   PUBMED   39009911
  REMARK    GeneRIF: Live Cell Protein Imaging of Tandem
            Complemented-GFP11-Tagged Coiled-Coil Domain-Containing Protein-124
            Identifies this Factor in G3BP1-Induced Stress-Granules.
REFERENCE   5  (bases 1 to 10227)
  AUTHORS   Li,L., Yu,S., Dou,N., Wang,X., Gao,Y. and Li,Y.
  TITLE     A new tandem repeat-enriched lncRNA XLOC_008672 promotes gastric
            carcinogenesis by regulating G3BP1 expression
  JOURNAL   Cancer Sci 115 (6), 1851-1865 (2024)
   PUBMED   38581120
  REMARK    GeneRIF: A new tandem repeat-enriched lncRNA XLOC_008672 promotes
            gastric carcinogenesis by regulating G3BP1 expression.
REFERENCE   6  (bases 1 to 10227)
  AUTHORS   Kociok,N., Esser,P., Unfried,K., Parker,F., Schraermeyer,U.,
            Grisanti,S., Toque,B. and Heimann,K.
  TITLE     Upregulation of the RAS-GTPase activating protein (GAP)-binding
            protein (G3BP) in proliferating RPE cells
  JOURNAL   J Cell Biochem 74 (2), 194-201 (1999)
   PUBMED   10404389
REFERENCE   7  (bases 1 to 10227)
  AUTHORS   Costa,M., Ochem,A., Staub,A. and Falaschi,A.
  TITLE     Human DNA helicase VIII: a DNA and RNA helicase corresponding to
            the G3BP protein, an element of the ras transduction pathway
  JOURNAL   Nucleic Acids Res 27 (3), 817-821 (1999)
   PUBMED   9889278
REFERENCE   8  (bases 1 to 10227)
  AUTHORS   Gallouzi,I.E., Parker,F., Chebli,K., Maurier,F., Labourier,E.,
            Barlat,I., Capony,J.P., Tocque,B. and Tazi,J.
  TITLE     A novel phosphorylation-dependent RNase activity of GAP-SH3 binding
            protein: a potential link between signal transduction and RNA
            stability
  JOURNAL   Mol Cell Biol 18 (7), 3956-3965 (1998)
   PUBMED   9632780
REFERENCE   9  (bases 1 to 10227)
  AUTHORS   Parker,F., Maurier,F., Delumeau,I., Duchesne,M., Faucher,D.,
            Debussche,L., Dugue,A., Schweighoffer,F. and Tocque,B.
  TITLE     A Ras-GTPase-activating protein SH3-domain-binding protein
  JOURNAL   Mol Cell Biol 16 (6), 2561-2569 (1996)
   PUBMED   8649363
REFERENCE   10 (bases 1 to 10227)
  AUTHORS   Onno,M., Nakamura,T., Hillova,J. and Hill,M.
  TITLE     Rearrangement of the human tre oncogene by homologous recombination
            between Alu repeats of nucleotide sequences from two different
            chromosomes
  JOURNAL   Oncogene 7 (12), 2519-2523 (1992)
   PUBMED   1461655
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU517281.1, BX647869.1,
            AK130003.1, BX648012.1 and AC091982.3.
            
            On Nov 23, 2018 this sequence version replaced NM_005754.2.
            
            Summary: This gene encodes one of the DNA-unwinding enzymes which
            prefers partially unwound 3'-tailed substrates and can also unwind
            partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.
            This enzyme is a member of the heterogeneous nuclear RNA-binding
            proteins and is also an element of the Ras signal transduction
            pathway. It binds specifically to the Ras-GTPase-activating protein
            by associating with its SH3 domain. Several alternatively spliced
            transcript variants of this gene have been described, but the
            full-length nature of some of these variants has not been
            determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) differs in the 5' UTR compared
            to variant 2. Variants 1 and 2 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK222606.1, SRR1660805.139079.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356245.8/ ENSP00000348578.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-605               BU517281.1         33-637
            606-2581            BX647869.1         516-2491
            2582-2794           AK130003.1         2105-2317
            2795-2796           BX648012.1         2811-2812
            2797-10227          AC091982.3         91290-98720         c
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.1"
     gene            1..10227
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="G3BP stress granule assembly factor 1"
                     /db_xref="GeneID:10146"
                     /db_xref="HGNC:HGNC:30292"
                     /db_xref="MIM:608431"
     exon            1..83
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            84..227
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1533
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /EC_number="3.6.4.13"
                     /EC_number="3.6.4.12"
                     /note="Ras-GTPase-activating protein SH3-domain-binding
                     protein; GAP binding protein; GTPase activating protein
                     (SH3 domain) binding protein 1; DNA helicase VIII;
                     RasGAP-associated endoribonuclease G3BP; G3BP-1;
                     ATP-dependent DNA helicase VIII; GAP SH3 domain-binding
                     protein 1"
                     /codon_start=1
                     /product="ras GTPase-activating protein-binding protein 1"
                     /protein_id="NP_005745.1"
                     /db_xref="CCDS:CCDS4319.1"
                     /db_xref="GeneID:10146"
                     /db_xref="HGNC:HGNC:30292"
                     /db_xref="MIM:608431"
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGG
                     LDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNN
                     QALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQ
                     QTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPV
                     LEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVK
                     VPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMV
                     RHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQ
                     KVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM
                     VQKPGFGVGRGLAPRQ"
     exon            228..309
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            310..483
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            484..574
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            575..671
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            672..873
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            874..975
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1087
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1216
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1326
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..10227
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2769..2774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="hexamer: ATTAAA"
     polyA_site      2791
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="major polyA site"
     regulatory      8774..8779
                     /regulatory_class="polyA_signal_sequence"
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="hexamer: AATAAA"
     polyA_site      8794
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="major polyA site"
     regulatory      9002..9007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="hexamer: AATAAA"
     polyA_site      9023
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
     regulatory      10195..10200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="hexamer: AATAAA"
     polyA_site      10227
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
ORIGIN      
        1 gtgcggacgc agttgcgtga ggggtttgta ctatcctcgg tgctgtggtg cagagctagt
       61 tcctctccag ctcagccgcg taggtttgga catatttgac tcttttcccc ccaggttgaa
      121 ttgaccaaag caatggtgat ggagaagcct agtcccctgc tggtcgggcg ggaatttgtg
      181 agacagtatt acacactgct gaaccaggcc ccagacatgc tgcatagatt ttatggaaag
      241 aactcttctt atgtccatgg gggattggat tcaaatggaa agccagcaga tgcagtctac
      301 ggacagaaag aaatccacag gaaagtgatg tcacaaaact tcaccaactg ccacaccaag
      361 attcgccatg ttgatgctca tgccacgcta aatgatggtg tggtagtcca ggtgatgggg
      421 cttctctcta acaacaacca ggctttgagg agattcatgc aaacgtttgt ccttgctcct
      481 gaggggtctg ttgcaaataa attctatgtt cacaatgata tcttcagata ccaagatgag
      541 gtctttggtg ggtttgtcac tgagcctcag gaggagtctg aagaagaagt agaggaacct
      601 gaagaaagac agcaaacacc tgaggtggta cctgatgatt ctggaacttt ctatgatcag
      661 gcagttgtca gtaatgacat ggaagaacat ttagaggagc ctgttgctga accagagcct
      721 gatcctgaac cagaaccaga acaagaacct gtatctgaaa tccaagagga aaagcctgag
      781 ccagtattag aagaaactgc ccctgaggat gctcagaaga gttcttctcc agcacctgca
      841 gacatagctc agacagtaca ggaagacttg aggacatttt cttgggcatc tgtgaccagt
      901 aagaatcttc cacccagtgg agctgttcca gttactggga taccacctca tgttgttaaa
      961 gtaccagctt cacagccccg tccagagtct aagcctgaat ctcagattcc accacaaaga
     1021 cctcagcggg atcaaagagt gcgagaacaa cgaataaata ttcctcccca aaggggaccc
     1081 agaccaatcc gtgaggctgg tgagcaaggt gacattgaac cccgaagaat ggtgagacac
     1141 cctgacagtc accaactctt cattggcaac ctgcctcatg aagtggacaa atcagagctt
     1201 aaagatttct ttcaaagtta tggaaacgtg gtggagttgc gcattaacag tggtgggaaa
     1261 ttacccaatt ttggttttgt tgtgtttgat gattctgagc ctgttcagaa agtccttagc
     1321 aacaggccca tcatgttcag aggtgaggtc cgtctgaatg tcgaagagaa gaagactcga
     1381 gctgccaggg aaggcgaccg acgagataat cgccttcggg gacctggagg ccctcgaggt
     1441 gggctgggtg gtggaatgag aggccctccc cgtggaggca tggtgcagaa accaggattt
     1501 ggagtgggaa gggggcttgc gccacggcag tgaatcttca tggatcttca tgcagccata
     1561 caaaccctgg ttccaacaga atggtgaatt ttcgacagcc tttggtatct tggagtatga
     1621 ccccagtctg ttataaactg cttaagtttg tataatttta ctttttttgt gtgttaatgg
     1681 tgtgtgctcc ctctccctct cttccctttc ctgaccttta gtctttcact tccaattttg
     1741 tggaatgata ttttaggaat aacggacttt taaagaagca aaaaaaaaga ctgaatttcc
     1801 ttgcttactt tgcatataca gactggattt tttttttttt tttacagcca tttccccaaa
     1861 ggaatgtctt gcatattact gacatttggt atgtttcatt cattggaata tttcttattt
     1921 tctacgtgtt tgaaaagcct gtaagaaata caggatttga taatattttg aaggcaggaa
     1981 aaacccaaat tgtttcttct ttgagagtca tgactacctt ctggtgtgga gaaattgcca
     2041 ttggaaaatt tgacaatttt gattctcact ggtatgttta aaaactgaat aaaaggaata
     2101 gaattttttt ttgataaagg atcacaaaac aattctaaaa cctaactgtt tttaccattg
     2161 aaatttaaat tgtgataata ggttttaaat gtctagaatg caactgatag gcttttcttg
     2221 aactgttagt ttttttgaag tagttttttc atgtttaatt tgtatttgta aaaaaacaaa
     2281 aagcaaaaaa attcccaaaa cccagataac aaccagagca aaactgttgt gccttctatt
     2341 tatctttgat ttcagtcttg gcaattgttt aaaaaaaaaa tctagatttg ttttattagg
     2401 ttcagagtat gtggggaatt atagaatccc tctttcatca ctttgtgtat gtcttttgtt
     2461 aacatatttg ttatgcctta ttctaaaatt gagtctcaaa ctggaatgcc tttgaagaca
     2521 gatgcttcta tagaggttct ttgacctaaa tagttcagca tttgtatttt tattctggta
     2581 tctaatcaga ttcctaatca tagcccgtaa gaaggaatgt tactttaata ttggactttg
     2641 ctcatgtgct cgtgtccgca tttttttttt tcttaaaatc atagccatat ggtaaatttt
     2701 ctattttgtt atggttctct tttattgatg ggcatgcagt gggtgttact tggaaatggc
     2761 caatttttat taaaatattt ctggaagaaa atttaatctg gcaatataga ctaatactcg
     2821 tttcacatat gtgttatttg ttgagtgctg tgtgtgagat gggtgaaggt tttgtttaca
     2881 cgtgtgaaac aaacttttgt gtgattgtca ttactaattg aagggcaacc aggttgtaaa
     2941 attcagcgta tttattttct taacagtatt catctttagg actgtcgttt gaggatagca
     3001 taggaatatt tggtttgcat tactgagaaa ctctaggttt gctgtgatgg ttaagaccac
     3061 agtgaagttg gtattaccag tttggttcca gttcctcaaa tatttgaaaa ttggcagcat
     3121 ctcctttaca taaacttaat gtaactgaat cacatggctt ttttgtaggg ttaaagtaac
     3181 tttggtaacc acttttggcc cagatcactg ataataggaa gaaatacaac ttatactttt
     3241 gtagcttata ttaagcatgt ggtgggtgga aagaaaagtt tagtctgagt cactttggaa
     3301 aaaaaaatta ctttgtggta acttcaaggt cataaattat ggatagccta gaaattatcc
     3361 ttcatgagtg aatactaaat tatacatttc aaaacagaag ttcttcctac ttggagactt
     3421 aagggcatta actagatgca gcaatcctca gcttggtgtt atcacatact agaggacatt
     3481 tggaaatgtg tgaaatttgg aagggttgtt ggagagacat gcatctggca tttattgggt
     3541 ggggtctaag gataccaaat gttgtgtaaa aatgaagcaa ccccattgag aacactgata
     3601 gtccctattc ctgtgggtgg gtggagctgt gggattagga aaactggaat gaagactcat
     3661 tcttttgaaa ctcagcctca gataaataac aaactaggaa atggaaatct gtttcaagaa
     3721 ttgcagtaac atctactcta aggaattagt aatttcactt tttattattt gtaccaaatg
     3781 tatgtgtgtg ttgtgtatgt tggtgtaaat gtggagaggt ggtagcaata catactgatg
     3841 ctgcttgtaa tttgttgcag ggaattactg cacaatctca gaaaatagaa agcttcccca
     3901 gtgctttctg tcatcagagt tgggatgatt gtgaggtttt gaatgatagt attggaccta
     3961 agaaatagga ttagcatcat gatcagaact gttgagaatc cttgtacaat tatgtacatt
     4021 attgcatgta gagtaaacct gagagcttag agatgtatac gtttccactg ctggaaaatc
     4081 attataagtt agggcaacat tttctatagg tggcagcagt aggagctcat cacattgaac
     4141 attttcagat tttaaaatga aaattttcat ccgtaggaat ggcccaatgt atagttatta
     4201 gctactgagc ttagaactct tgacccatga gatttttttt ttaaattttt atttttatag
     4261 aggcaaggtc ttgctatgtt gtcaggctgg tctcaagctc ctggcctcat cagttctcct
     4321 gccttccaag tagccggaac tacaggtgtg caccattgca cctggcctca tgagtatttt
     4381 agttagataa gatttgtttg gttcagtggg tcttagtttt ttaagacctg ttaatttgta
     4441 attagacatc ttgggcctta aactgtattt ttgttttagt ggtgaggttg ggttttactt
     4501 tgtgtattcc agcgttctca actttgtcag atattgctag ggaccagtga gatagataat
     4561 ccaaaaataa taatttgact ttggttgagt atgtcattac cacagtgtta gttgaattgc
     4621 actttaggtt agaaaagtta attttcacat ctgtgctgcg ggttaggtga tattgtcctc
     4681 agcacaatag atgaggaaga aactgaatca gaacagttaa atgacaggct tccaagtcat
     4741 ccatgtagca aggagcacat ttgcctccca aactgatgct ttttctctag aaccataatg
     4801 cccccctaat tacatttcag tagcatagac cgcaatgaac ttcctactgt agggagactt
     4861 gagtctggaa tacattcaga acaaggttaa cattacagtt cttaatttcc tttagggcaa
     4921 acaagataat taaacttggg ttttgtgagt ctttttttga tttgcagatt atatatggta
     4981 acacaataat ggtggtttgt ttttaagttt tttctcatca ggtttgataa tttaatgcaa
     5041 acttcaaatc tggatgtgaa taataatatt tttattatag tataatccag gtgagtttat
     5101 agaatttgtg ttttgtgctt ataaactgtc accttctgat acttttcttc atccctctat
     5161 ttgaaaaacc cttgtagaat ttaacgattt aatggaaaat gttgcacttt atagaaggcc
     5221 attttagaac ttacaggtaa cctttttgaa gagcttagat ttgtagacca ctgtttatgc
     5281 tgagtgacat ttcttttaaa tggttgcatt tgtcaaatct ataaagatgt tttttatatt
     5341 gcagttttct ttttgtgcta cttaaaggaa tctttgcaat gctgcatata gataattgtt
     5401 gaccttttta gttcagcagt ttttaaactt gaagtaacat cagcatttat ttgaatatca
     5461 ggttttagct aactgcctaa tgtaccaaaa aaaaattagc aatgtcttat gtgggatgag
     5521 gggttaagtt aaagttgctg atcaagtaat ggccgtgtgg cacaagatga taactaagaa
     5581 tttctggttc taaggtagta atgccttttg ggatttatat ttgtttaatg ttgagattct
     5641 ggggaccata ggtgggcctg tcaactctta acagactata gtaaaggaga gatggtggca
     5701 catgtgagat aaaacttgtt tatatttaca ttcttattag tggatagata tcacctcagt
     5761 acaacactga aattaaactg ccttgttcat gctttcatgt ctcaaatcaa ggcctaaatg
     5821 agtaaaaaga tgattacaga ttacctaaaa cccttaacca gttttggttt ttgattatgc
     5881 atatgtgttg gcttgcaatt aggtttttag tttctgttta tgtgacttgt ttgaaaacat
     5941 tggattcgtc ttctctccac agtttctact tgaacctagt agcttatttt aattccctgt
     6001 aagaggaaaa taatataggg aatctgttca actattcctt taataaatca tgtgacttgg
     6061 ccaattacag aaattcctaa gtgtagtatt ttgaaatgtg gccagtgtcc cagatttgta
     6121 aggtacagtg tgcatacagt gtgtcttagg atttttttaa gtaaattaag gatgtttacc
     6181 agaaattatt gatttactga accaagtatt tattttcaga agcccacttt tggggaaggt
     6241 tgcctatatt tttgatagta attagatgga agattaaatc ctgtttaacc tggatagaaa
     6301 cagttttaac tattgctgaa gttaaaatca ctttggggtg gggtggggtg gctcatgcct
     6361 gtaatcccag tgctgtggga ggctgaggcc agaggattgc tcgaggccag gaatttgaga
     6421 ccagcttggg caacatagca agaccctgtc tctacagata attgaaaagt gagccagatg
     6481 tggtggcgca ttcctatagt ctccgtctct caggaggctg aggcaggaga ttgcttgagc
     6541 ccaggagttg gaggctgcag tgagttatga tcataccact gcactctaac ctgggtgaca
     6601 gagtgagacc ctgtctctaa aaaacaaaaa attagaaaac aacaaaaacc acttcggtta
     6661 ctcactgtcc tcatgctttg atgtcagttg ttctcctaaa ttttaaaatt ttgttatgaa
     6721 gattggatgt acactgattt ggccctttaa atgacattat accaagaagt tggtataagg
     6781 gagtaggaga tgagaaaact ttttgaaaaa tcatttggtg catgctaagg tagcttgtag
     6841 gacatgtggt tttaaaaaat tcatggattt tgaagaagga tagggtggaa gtaaaattta
     6901 gctgatagca tagggatcat ttgtgattta tatagaaaat aattatattt ttgagtgaga
     6961 agcatcagac ttaaattttt ttcttctatt ttggttatta ccaaaaggaa cagagaaact
     7021 ctcagaattc tttgaatcat agaatgttag tgctggaaag gactaggaag tgacttgtac
     7081 aaccccttca ttttacaaag gatcagagag aattggttag tagagtccca aatctgcttt
     7141 tagaagtaat atggtgatca tcagtttaga ttcactgtag gaagggcagc cctgttaaag
     7201 tttgtcagga gaaaaaggga aggtatcact ttttgtatgt tcacccagtt gcttctgtag
     7261 ttttcatacc cctgttttct caattttctt agaactgtct ttaatggccc agctctacca
     7321 gggcttgttg tctaaggaca ttaacttgtg ctcccctcag ggatgggttt actactagct
     7381 gtcagaaagc tattgggtat cctaatgtgt taatagctga aactcagctg taatttctcc
     7441 taaatacttc agcattttgc attctgtaca ttgtggtgct ttttccacct tgtattgttg
     7501 taactgtaag ctcctagggg gcagcaattt ggtcttaggc gtgtaccctg tttagtgcct
     7561 ggcaatgcca tgtttatatc aaggtcttaa tattactttt gagttatttc tcccactctt
     7621 catggaagtg atgctccagc cttgctaaaa cactgagggt cctcccgata agccaggcat
     7681 tgtgattcct tggcttcaag tgccttctgt cttccccgaa ggtccactga gatctacaat
     7741 cccccctttc ctctgatctc ttgccatgta agtaattttt taaaagatca gtacaagagc
     7801 tatatttttt cctaagaaaa aatttcaaca ggaatgggga gtagccccgt tttggggctg
     7861 atctgtccag aatttggagt gaggaacaaa tccattctca tctactttcc acattttccc
     7921 tcttgtttga ggtcctgttc caaccttcat ttctgaaact gttctagagc actttgtctt
     7981 tctcgtagtt cataacttac cccttcagtc tagaattaga attacattat ctgttttact
     8041 actttactag actgtaagct cctagaagat aaggactagg gagttcatct ctgtattcca
     8101 ccagaaggta cagtgactca taactagagt ctttagatga aacttactga gttgaataac
     8161 ttaatatatt tctgttttca ttcccaaggg aggccatgtc tggagataga ccttgaattt
     8221 aataaatttt aggcactata ccatttcagt ggagaaaatt gttgggaaat ttggggggat
     8281 ggatatataa gggggaggaa gtcactggcc agtttgaggt gcttccattg gtctgggtaa
     8341 ctagccattt cttcctgatt gtgcctagta tatcccagac agtttgtttc tatgcagaag
     8401 aattttatat gaaattttca tttctttgca aaaacttgtt ccttctttca gttcatgaca
     8461 tcatccatgc taaagtctga gtcatctgct tccatttttg ttattcccca catccagtct
     8521 tcagctaagt cctgtcagtt ctacctcaag tctttcccgt ctttcttcct cacctatgta
     8581 atttcagtag tctctcagct tcacttaacc tcttactcca gcctacttta tacacaatgt
     8641 tagattcatc ttactgaaac atagttctga ccatgtattc catgtatgtg ttctctctcc
     8701 cctattagac tgtaagctcc ttatgggcag gatctgtgtc tgagtcatct ttgtatcttg
     8761 cctagcacct atcaataaat acttcttgaa tgaacaaatg aattctcatt tctcgacata
     8821 aagccttgtc taaagtgaga tgttttccag gtcatacata ggtttctccc tggggtaatt
     8881 ggaagttctt gcatataaag ttggttaaat agtaataatt atgagttaac cacagaaaat
     8941 ttcagaaaac tttaattttt ttccattgga atcagcatgg atatgtgtat atggatggtt
     9001 caataaatat acttttatat acatgtttca cagaaatttt acattccatc ttcaaactgc
     9061 agaaatagat tttttttttt tacaagccaa aaaataactg ccataacttt tgtgacttca
     9121 tgcaaaaaaa ggggggaaga gtaaggggca aaagggaatt agttgttcaa ggttgagtgt
     9181 ccagtaagaa gtaaaaatgt tacattaagg aagcgattgc ttaacacgct gcttaaggtg
     9241 gtagccagac aaatccttaa ctgcagcatt aaggccatac caagtttata catatataca
     9301 aagaaatttc catataaaag acatactatc acattttctt cttcataagg atgaggaact
     9361 ttgtaattct gacctgattt tccaggccaa ttaattaaaa tgtccccagg ctgcttttta
     9421 gaagtatctt ctactttggc tagctgtcag agttgccgct atgtgagctt gtgacttctg
     9481 gtctggcgtg aaagtatgaa tcatcttgaa cctcttccat catagcacta tgttgctgag
     9541 ctaattcatg ctgcccagct aagcacattt tctacatccc caaagtattt ggaatcacag
     9601 cattgtaact tgtgattgtc ttacctgtaa cagactccac agtcatgctg gcattgtgac
     9661 ctgaagaatt acacagtgcc cttcttagat tctttggggt taaggtaact tcttgaataa
     9721 tgtactaatg aacgatagtg tttcaggaaa catgccctag ctattggaag ttcgtatcta
     9781 acttttaccc cttgtgtgtc atgatacatt taagttggta gctacatttt tggaataaag
     9841 gaaatgtaaa attatttgca gaataataac ctgggatttg ctcttctgcc taaacatcga
     9901 actagggaac tttaaagaca aagggtgcat cttttgtgca agtatctaag cgggcaaaat
     9961 ggggaaaaca tgaagggcaa gtcctttggg gatgacttct aattaatgca gaagtcattt
    10021 gctttggcca ggttgggtgg gactctgaga ttgcacactc ttgccctgtt ggcagaaact
    10081 agcccatctt aagttagtca atgaatcctg agtcaaaatg aaaggttgct aaatctaaga
    10141 tcgagtgtat aaataaaata accccttcct ccagtgactg tgcatttatt ttgtaataaa
    10201 ctcattttgg aatgttttag caatgaa
//
