LOCUS       NM_024513               8514 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens FYVE and coiled-coil domain autophagy adaptor 1
            (FYCO1), transcript variant 1, mRNA.
ACCESSION   NM_024513
VERSION     NM_024513.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Sun,X., Zhou,L., Wang,X., Li,Y., Liu,X., Chen,Y., Zhong,Z. and
            Chen,J.
  TITLE     FYCO1 regulates migration, invasion, and invadopodia formation in
            HeLa cells through CDC42/N-WASP/Arp2/3 signaling pathway
  JOURNAL   Biochem Cell Biol 100 (6), 458-472 (2022)
   PUBMED   36342046
  REMARK    GeneRIF: FYCO1 regulates migration, invasion, and invadopodia
            formation in HeLa cells through CDC42/N-WASP/Arp2/3 signaling
            pathway.
REFERENCE   3  (bases 1 to 8514)
  AUTHORS   Mei,S., Lin,J., Liu,Z. and Li,C.
  TITLE     A Novel Mutation in the FYCO1 Gene Causing Congenital Cataract:
            Case Study of a Chinese Family
  JOURNAL   Dis Markers 2022, 5838104 (2022)
   PUBMED   36061348
  REMARK    GeneRIF: A Novel Mutation in the FYCO1 Gene Causing Congenital
            Cataract: Case Study of a Chinese Family.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8514)
  AUTHORS   Ellinghaus,D., Degenhardt,F., Bujanda,L., Buti,M., Albillos,A.,
            Invernizzi,P., Fernandez,J., Prati,D., Baselli,G., Asselta,R.,
            Grimsrud,M.M., Milani,C., Aziz,F., Kassens,J., May,S., Wendorff,M.,
            Wienbrandt,L., Uellendahl-Werth,F., Zheng,T., Yi,X., de Pablo,R.,
            Chercoles,A.G., Palom,A., Garcia-Fernandez,A.E.,
            Rodriguez-Frias,F., Zanella,A., Bandera,A., Protti,A., Aghemo,A.,
            Lleo,A., Biondi,A., Caballero-Garralda,A., Gori,A., Tanck,A.,
            Carreras Nolla,A., Latiano,A., Fracanzani,A.L., Peschuck,A.,
            Julia,A., Pesenti,A., Voza,A., Jimenez,D., Mateos,B., Nafria
            Jimenez,B., Quereda,C., Paccapelo,C., Gassner,C., Angelini,C.,
            Cea,C., Solier,A., Pestana,D., Muniz-Diaz,E., Sandoval,E.,
            Paraboschi,E.M., Navas,E., Garcia Sanchez,F., Ceriotti,F.,
            Martinelli-Boneschi,F., Peyvandi,F., Blasi,F., Tellez,L.,
            Blanco-Grau,A., Hemmrich-Stanisak,G., Grasselli,G., Costantino,G.,
            Cardamone,G., Foti,G., Aneli,S., Kurihara,H., ElAbd,H., My,I.,
            Galvan-Femenia,I., Martin,J., Erdmann,J., Ferrusquia-Acosta,J.,
            Garcia-Etxebarria,K., Izquierdo-Sanchez,L., Bettini,L.R., Sumoy,L.,
            Terranova,L., Moreira,L., Santoro,L., Scudeller,L., Mesonero,F.,
            Roade,L., Ruhlemann,M.C., Schaefer,M., Carrabba,M.,
            Riveiro-Barciela,M., Figuera Basso,M.E., Valsecchi,M.G.,
            Hernandez-Tejero,M., Acosta-Herrera,M., D'Angio,M., Baldini,M.,
            Cazzaniga,M., Schulzky,M., Cecconi,M., Wittig,M., Ciccarelli,M.,
            Rodriguez-Gandia,M., Bocciolone,M., Miozzo,M., Montano,N.,
            Braun,N., Sacchi,N., Martinez,N., Ozer,O., Palmieri,O., Faverio,P.,
            Preatoni,P., Bonfanti,P., Omodei,P., Tentorio,P., Castro,P.,
            Rodrigues,P.M., Blandino Ortiz,A., de Cid,R., Ferrer,R.,
            Gualtierotti,R., Nieto,R., Goerg,S., Badalamenti,S., Marsal,S.,
            Matullo,G., Pelusi,S., Juzenas,S., Aliberti,S., Monzani,V.,
            Moreno,V., Wesse,T., Lenz,T.L., Pumarola,T., Rimoldi,V., Bosari,S.,
            Albrecht,W., Peter,W., Romero-Gomez,M., D'Amato,M., Duga,S.,
            Banales,J.M., Hov,J.R., Folseraas,T., Valenti,L., Franke,A. and
            Karlsen,T.H.
  CONSRTM   Severe Covid-19 GWAS Group
  TITLE     Genomewide Association Study of Severe Covid-19 with Respiratory
            Failure
  JOURNAL   N Engl J Med 383 (16), 1522-1534 (2020)
   PUBMED   32558485
REFERENCE   5  (bases 1 to 8514)
  AUTHORS   Rothwell,S., Lilleker,J.B. and Lamb,J.A.
  TITLE     Genetics in inclusion body myositis
  JOURNAL   Curr Opin Rheumatol 29 (6), 639-644 (2017)
   PUBMED   28777108
  REMARK    Review article
REFERENCE   6  (bases 1 to 8514)
  AUTHORS   Chen,J., Ma,Z., Jiao,X., Fariss,R., Kantorow,W.L., Kantorow,M.,
            Pras,E., Frydman,M., Pras,E., Riazuddin,S., Riazuddin,S.A. and
            Hejtmancik,J.F.
  TITLE     Mutations in FYCO1 cause autosomal-recessive congenital cataracts
  JOURNAL   Am J Hum Genet 88 (6), 827-838 (2011)
   PUBMED   21636066
  REMARK    GeneRIF: FYCO1 is involved in lens development and transparency in
            humans, and mutations in this gene are one of the most common
            causes of autosomal-recessive congenital cataracts in the Pakistani
            population.
REFERENCE   7  (bases 1 to 8514)
  AUTHORS   Pankiv,S. and Johansen,T.
  TITLE     FYCO1: linking autophagosomes to microtubule plus end-directing
            molecular motors
  JOURNAL   Autophagy 6 (4), 550-552 (2010)
   PUBMED   20364109
REFERENCE   8  (bases 1 to 8514)
  AUTHORS   Pankiv,S., Alemu,E.A., Brech,A., Bruun,J.A., Lamark,T.,
            Overvatn,A., Bjorkoy,G. and Johansen,T.
  TITLE     FYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate
            microtubule plus end-directed vesicle transport
  JOURNAL   J Cell Biol 188 (2), 253-269 (2010)
   PUBMED   20100911
  REMARK    GeneRIF: We have characterized the LC3-, Rab7-, and
            phosphatidylinositol-3-phosphate-binding domains in FYCO1 and
            mapped part of the CC region essential for MT plus end-directed
            transport.
REFERENCE   9  (bases 1 to 8514)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   10 (bases 1 to 8514)
  AUTHORS   Kiss,H., Yang,Y., Kiss,C., Andersson,K., Klein,G., Imreh,S. and
            Dumanski,J.P.
  TITLE     The transcriptional map of the common eliminated region 1 (C3CER1)
            in 3p21.3
  JOURNAL   Eur J Hum Genet 10 (1), 52-61 (2002)
   PUBMED   11896456
  REMARK    GeneRIF: Maps to a region of chromosome 3p21.3 which is frequently
            deleted in tumors.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB134257.1, BC101468.1 and
            AC099782.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024513.3.
            
            Summary: The gene encodes a Rab7 adapter protein that is implicated
            in the microtubule transport of autophagosomes. The encoded protein
            contains a RUN domain, a FYVE-type zinc finger domain, and Golgi
            dynamics (GOLD) domain. The encoded protein plays a role in
            microtubule plus end-directed transport of autophagic vesicles
            through interactions with the small GTPase Rab7,
            phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker
            LC3, and the kinesin KIF5. Mutations in this gene are associated
            with inclusion body myositis (IBM) and autosomal recessive
            congenital cataracts (CATC2). [provided by RefSeq, Aug 2020].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.20762.1, AJ292348.2
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: locus in the vicinity of
                                      disease-associated variant(s)
            MANE Ensembl match     :: ENST00000296137.7/ ENSP00000296137.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               DB134257.1         1-130
            131-4705            BC101468.1         1-4575
            4706-8514           AC099782.2         103828-107636       c
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..8514
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /note="FYVE and coiled-coil domain autophagy adaptor 1"
                     /db_xref="GeneID:79443"
                     /db_xref="HGNC:HGNC:14673"
                     /db_xref="MIM:607182"
     exon            1..103
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            104..270
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..4652
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /note="isoform 1 is encoded by transcript variant 1; RUN
                     and FYVE domain containing 3; FYVE and coiled-coil
                     domain-containing protein 1; zinc finger FYVE
                     domain-containing protein 7; FYVE and coiled-coil domain
                     containing 1"
                     /codon_start=1
                     /product="FYVE and coiled-coil domain-containing protein 1
                     isoform 1"
                     /protein_id="NP_078789.2"
                     /db_xref="CCDS:CCDS2734.1"
                     /db_xref="GeneID:79443"
                     /db_xref="HGNC:HGNC:14673"
                     /db_xref="MIM:607182"
                     /translation="MASTNAESQLQRIIRDLQDAVTELSKEFQEAGEPITDDSTSLHK
                     FSYKLEYLLQFDQKEKATLLGNKKDYWDYFCACLAKVKGANDGIRFVKSISELRTSLG
                     KGRAFIRYSLVHQRLADTLQQCFMNTKVTSDWYYARSPFLQPKLSSDIVGQLYELTEV
                     QFDLASRGFDLDAAWPTFARRTLTTGSSAYLWKPPSRSSSMSSLVSSYLQTQEMVSNF
                     DLNSPLNNEALEGFDEMRLELDQLEVREKQLRERMQQLDRENQELRAAVSQQGEQLQT
                     ERERGRTAAEDNVRLTCLVAELQKQWEVTQATQNTVKELQTCLQGLELGAAEKEEDYH
                     TALRRLESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMAQQKADTASDTKGRQEPI
                     PSDAAQEMQELGEKLQALERERTKVEEVNRQQSAQLEQLVKELQLKEDARASLERLVK
                     EMAPLQEELSGKGQEADQLWRRLQELLAHTSSWEEELAELRREKKQQQEEKELLEQEV
                     RSLTRQLQFLETQLAQVSQHVSDLEEQKKQLIQDKDHLSQQVGMLERLAGPPGPELPV
                     AGEKNEALVPVNSSLQEAWGKPEEEQRGLQEAQLDDTKVQEGSQEEELRQANRELEKE
                     LQNVVGRNQLLEGKLQALQADYQALQQRESAIQGSLASLEAEQASIRHLGDQMEASLL
                     AVRKAKEAMKAQMAEKEAILQSKEGECQQLREEVEQCQQLAEARHRELRALESQCQQQ
                     TQLIEVLTAEKGQQGVGPPTDNEARELAAQLALSQAQLEVHQGEVQRLQAQVVDLQAK
                     MRAALDDQDKVQSQLSMAEAVLREHKTLVQQLKEQNEALNRAHVQELLQCSEREGALQ
                     EERADEAQQREEELRALQEELSQAKCSSEEAQLEHAELQEQLHRANTDTAELGIQVCA
                     LTVEKERVEEALACAVQELQDAKEAASREREGLERQVAGLQQEKESLQEKLKAAKAAA
                     GSLPGLQAQLAQAEQRAQSLQEAAHQELNTLKFQLSAEIMDYQSRLKNAGEECKSLRG
                     QLEEQGRQLQAAEEAVEKLKATQADMGEKLSCTSNHLAECQAAMLRKDKEGAALREDL
                     ERTQKELEKATTKIQEYYNKLCQEVTNRERNDQKMLADLDDLNRTKKYLEERLIELLR
                     DKDALWQKSDALEFQQKLSAEERWLGDTEANHCLDCKREFSWMVRRHHCRICGRIFCY
                     YCCNNYVLSKHGGKKERCCRACFQKLSEGPGSPDSSGSGTSQGEPSPALSPASPGPQA
                     TGGQGANTDYRPPDDAVFDIITDEELCQIQESGSSLPETPTETDSLDPNAAEQDTTST
                     SLTPEDTEDMPVGQDSEICLLKSGELMIKVPLTVDEIASFGEGSRELFVRSSTYSLIP
                     ITVAEAGLTISWVFSSDPKSISFSVVFQEAEDTPLDQCKVLIPTTRCNSHKENIQGQL
                     KVRTPGIYMLIFDNTFSRFVSKKVFYHLTVDRPVIYDGSDFL"
     exon            271..377
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            378..503
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            504..610
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            611..754
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            755..845
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            846..3272
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            3273..3365
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3484
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            3485..3652
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            3653..3802
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            3803..4014
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            4015..4159
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            4160..4255
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            4256..4466
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            4467..4576
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     exon            4577..8514
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8490..8495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /note="hexamer: AATAAA"
     polyA_site      8514
                     /gene="FYCO1"
                     /gene_synonym="CATC2; CTRCT18; RUFY3; ZFYVE7"
                     /note="major polyA site"
ORIGIN      
        1 accctcttgg tgtgcgcgtc atggccgtca gcaccgcgtt cccgtcctct tccgcttggc
       61 cccagaaagt ttcggttctg cccggcggtg gacccacgag cgcgtgccac catggagtct
      121 gaccactgct gagcagacag ccaccgaggg ccgaaattct gagccttcct ctggacccag
      181 gcaggagaca tacagacaag aaaggcaaac tcaccatggc ctccaccaat gcagagagcc
      241 agctccagag aatcatccga gacttgcaag atgctgtgac agaactaagc aaagaatttc
      301 aggaagcagg ggaacccatc acggatgaca gcaccagctt gcataaattt tcttataaac
      361 ttgagtatct cctgcaattt gatcagaaag agaaggccac cctcctgggc aacaagaagg
      421 actactggga ttacttctgt gcctgcctgg ccaaggtgaa aggagccaat gatgggatcc
      481 gctttgtcaa gtctatctca gagctccgaa catccttggg gaaaggaaga gcatttattc
      541 gctactcctt ggtgcaccag aggttggcag acaccttaca gcagtgcttc atgaacacca
      601 aagtgaccag tgactggtac tatgcaagaa gcccctttct gcagccaaag ctgagctcgg
      661 acattgtggg ccaactctat gagctgactg aggttcagtt tgacctggcg tcgaggggct
      721 ttgacttgga tgctgcctgg ccaacatttg ccaggaggac gctgaccact ggctcttctg
      781 cttacctgtg gaaaccccct agccgcagct ccagcatgag cagcttggtg agcagctacc
      841 tgcagactca agagatggtg tccaactttg acctgaacag ccccctaaac aacgaggcat
      901 tggagggctt tgatgagatg cgactagagc tggaccagtt ggaggtgcgg gagaagcagc
      961 tacgggagcg catgcagcag ctggacagag agaaccagga gctgagggca gctgtcagcc
     1021 agcaagggga gcaactgcag acagagaggg agagggggcg cactgcagcg gaggacaacg
     1081 ttcgcctcac ttgcttggta gctgagctcc agaagcagtg ggaggtcacc caggccaccc
     1141 agaacactgt gaaggagctg cagacatgcc tgcagggcct ggagctagga gcagcagaga
     1201 aggaggagga ctaccacaca gccctgcggc ggctggagtc catgctgcag cccttggcac
     1261 aggagcttga ggccacacgg gactcactgg acaagaaaaa ccagcattta gccagcttcc
     1321 caggctggct agccatggct cagcagaagg cagatacggc atcagacaca aagggccggc
     1381 aagaacctat tcccagtgat gcggcccagg agatgcagga gctaggggag aagcttcaag
     1441 ccctagaaag ggagagaacc aaggtcgagg aggtcaacag acagcagagt gcccaactgg
     1501 aacagctggt caaggagctt cagctgaaag aggatgcccg ggccagcctg gagcgcctgg
     1561 tgaaggagat ggccccactc caggaggagt tgtctgggaa gggacaggag gcagaccagc
     1621 tctggcgacg gctgcaggag ttgctggccc acacgagctc ctgggaggag gagctagcag
     1681 agttgaggcg ggagaaaaaa cagcaacagg aggagaagga gctgctggag caggaggtca
     1741 ggtctctgac ccggcagctg cagttcctgg agacccagct ggcacaggtg agccaacatg
     1801 tgagtgacct ggaggagcag aagaagcagc tcattcagga caaagaccac ctcagccagc
     1861 aggtgggtat gctcgagcgg cttgctgggc cgcctggccc agaactgcca gtggcaggtg
     1921 agaagaatga ggccctggtc cctgtgaact ccagtctgca agaggcctgg gggaagccag
     1981 aggaggagca gaggggcctg caggaggcac agttagacga taccaaggtg caagagggca
     2041 gccaggagga agagctccgg caggccaaca gggagctgga gaaggagcta cagaatgtgg
     2101 tcgggcgtaa ccagctcctg gagggcaagc tgcaagccct gcaggccgat taccaggctt
     2161 tgcagcagcg ggaatcagcc atccagggct ccttggcctc cctggaggcc gagcaggcca
     2221 gcatccggca cttgggtgac cagatggagg cgagcttgct ggctgtaagg aaggccaagg
     2281 aggccatgaa agcccagatg gcagagaagg aggccattct acagagcaag gagggcgagt
     2341 gtcagcagct gcgggaggag gtggagcagt gccagcaact ggcagaagcc cggcacagag
     2401 agcttagggc tctcgagagc cagtgccagc agcagaccca gctgattgag gtcctcacag
     2461 cagagaaagg ccaacaggga gttggcccac ccactgacaa tgaagcccgt gagctggctg
     2521 cccagctagc cctgtctcag gcgcagctgg aagtccatca gggggaggtc caacggctgc
     2581 aggctcaggt ggtggacctc caggccaaga tgcgggcagc cctggatgac caggacaagg
     2641 tgcagagcca gctaagcatg gctgaggccg tcctgaggga gcacaaaacc cttgtgcagc
     2701 agctgaagga gcagaatgaa gcccttaaca gagcccatgt ccaggagctg ctgcaatgct
     2761 cggagcgtga aggggcactg caggaggaga gggccgatga ggcccagcag agggaggagg
     2821 agctgcgggc cctgcaggag gagctgtccc aggccaaatg cagctccgag gaagcacagc
     2881 tggagcacgc tgagctgcaa gagcagctgc accgggccaa cacagacaca gctgagctgg
     2941 gcatccaggt ttgcgcactg accgtggaaa aggagcgagt ggaggaggca ctggcctgtg
     3001 ctgtccagga gctccaggac gccaaagagg cagcctcaag ggagcgagag ggcctggagc
     3061 gccaagtagc tgggctgcag caagagaagg agagcttgca ggagaagctg aaggcggcca
     3121 aggcagcagc cggctcactg cctggcctgc aggcccagct cgcccaggca gagcagcggg
     3181 cccagagcct ccaagaggct gcacaccagg agctcaacac cctcaagttc cagctgagtg
     3241 ctgaaatcat ggactaccag agcagactta agaatgctgg tgaagagtgc aagagcctca
     3301 ggggccagct tgaggagcaa ggccggcagc tgcaggctgc tgaggaagct gtggagaagc
     3361 tgaaggccac ccaagcagac atgggagaga agctgagctg cactagcaac catcttgcag
     3421 agtgccaggc ggccatgctg aggaaggaca aggagggggc tgccctgcgt gaagacctag
     3481 aaaggaccca gaaggaactc gaaaaagcca caacaaaaat ccaagagtat tacaacaaac
     3541 tctgccagga ggtgacaaat cgtgagagga atgaccagaa gatgcttgct gacctggatg
     3601 acctcaacag aaccaagaag tatctcgagg agcggctgat agagctgctc agggacaagg
     3661 atgctctctg gcagaagtca gatgccctgg aattccagca gaagctcagt gctgaggaga
     3721 gatggctcgg agacacagag gcaaaccact gcctcgactg taagcgggag ttcagctgga
     3781 tggtgcggcg gcaccactgc aggatatgtg gccgcatctt ctgttactac tgctgcaaca
     3841 actacgtcct gagcaagcac ggtggcaaaa aggagcgctg ctgccgagcc tgtttccaga
     3901 agctcagtga aggccctggc tcccctgata gcagtggctc aggcactagc cagggagagc
     3961 ccagccctgc actgtcacca gcctcacctg ggccccaggc cacaggaggc caaggagcaa
     4021 atacagacta caggccaccg gacgacgctg tgtttgatat catcacagat gaggaattgt
     4081 gccagataca ggagtccggc tcctctttgc ctgaaacacc cactgaaact gattctctcg
     4141 acccaaatgc ggctgaacag gatactacat caacctcgct aacgcctgag gacactgaag
     4201 acatgcccgt ggggcaggat tcggaaatct gcctgctgaa gtctggagaa ctgatgatca
     4261 aagtacccct cacagtggat gagatcgcca gcttcgggga gggtagcagg gagctgtttg
     4321 tgaggtccag cacctacagc ctgatcccca tcactgtggc cgaggcaggc ctcaccatca
     4381 gctgggtctt ctcctctgac cccaagagca tctccttcag tgtggtcttc caggaggccg
     4441 aggacacacc gctggatcag tgtaaggtcc tcattcccac gacccgatgc aactcccaca
     4501 aggagaacat ccagggccag ctcaaggttc gcacacccgg catctacatg ctcatcttcg
     4561 acaatacctt ctcaaggttt gtctctaaaa aggtatttta tcacttgacg gttgatcggc
     4621 ctgtgatcta cgatggaagt gatttcctgt agcttcagca cctcagtaac ttcacttcat
     4681 ccacaggaaa cactgctctt cctcacctgt cacataaagc atttttttaa aaagtcagct
     4741 gctccaaaat catcaactca gcccctgggc tgcccctcag aggcggtgtc tggggaggac
     4801 tttgtgctca gcactctgca ccggccactc ttagcccccg aggcgttgaa gggctcaggc
     4861 aatgtttcca ttaagtagag actcagctgt tgtcacaccc aaagggatgc tctgccaaag
     4921 gtttaaacac ccaggagacc atcagcctct cctgggagca cagttggcta caggcctctt
     4981 gtggagagtt tcacgggcag gggtgattcc aacttctgcc tgtggagaga ttttctgccc
     5041 tgccccacca gggccctgca tgttggagac tgagctgggt gcactggcca tgccctgtga
     5101 atcctcaggc tgtgacgccc tcaggtactc ctgggaaaag gaggtacaca gccatcatgc
     5161 gagtcggtgc caggggaccc cccggagatc ctgaccagct cctccagtca tgctcttgtc
     5221 cctcactgcc ccagtaagct ggaggctgct ccagaactca gcagtgttgg aggggcctct
     5281 aagctgcact ctctttctgg cccttttgtc tgggtgattc tgtcctcaaa taaagccctt
     5341 cactcagcca gacctctcca cagctcaaag cattgcccta agaatcagaa gtaaagataa
     5401 tccaagagca aaacccactg tacttggggc ctgcaatggc tgtgtgtaca ctacatctaa
     5461 tgcccaaatg ccagccagtg tggatgttgt gaccacagag caggattgtg cattggcttt
     5521 agagctactc ctcagctgat ggcccacttt tgtttatata aataagagct tctgccccac
     5581 ctgcagacat gtttactaat gatcatagcc aggattagaa ccactttcaa acattggggc
     5641 cttcttaaca aaagtctttg ataacttaag aaccaaagta acagagtaaa cagaggcatg
     5701 atggatccct gggccccact cccctcctga caggttcccc aacagcccat ttgcccactt
     5761 cccactgctc agcccacacc agacctccag gagacatccc cccttgaggc agagagatcc
     5821 tgttccctat tcccagacaa gaattattta atcttccctg ttctctgtgg tccttttctt
     5881 ccccaacaac agatagctca ccttggacag ctcttcgtcc cttgttcatg gaaccagctg
     5941 cctgcagtca ggccccaggt tcttccatgg gtgaacagag catctgacaa aaggtcccag
     6001 tttggccagg ggtgagggag agagcaccag acaggctatc cgagaatctg agagctgggc
     6061 ccggcagttc ctccagctac ccttgtgacc taagtccagt cacacatttc ccaaagtttc
     6121 tctttgtcat aaccctggtc tggctggttt tgagggcttg agaatgggtc agggactcca
     6181 ggccaagtcc aacagagacc ccaaacccac cacacaccag cagccacaac ctcaccacca
     6241 acaaagagga cttttgtggg gccacaagta agaggtcatt tctggaatgg actcagacct
     6301 ttaaacagga gagttgagca cttccagtca gtttttaagc aaggcatggg gaacagggaa
     6361 tagaaccttt caaagaggtt gcccagagaa aagctgggcc tcttgcattc ggcttccttg
     6421 gagcagcctc ttctggcaga aagccatcag gtgctcaatc atcttctcct ggccaaggct
     6481 ctgaccatgc ttagtactgg aatagaggtg gccaggcccc cagcgactct tcttggcctg
     6541 atgtttgtcc tcacaggcat gccacgtggc ctgagatgat tcagaacaaa tcatgctaac
     6601 tttgaatcca tccagccact tgcaaatgat aatcagaagt cagcttgttc actgttagaa
     6661 agaaactaac aaaagagaac ccagagcaat ctagaatctt tgagtgcttg gctttccaag
     6721 gatactgcgg agactctggc caagctgatg accttctgaa gtgtcactgg caccatatgc
     6781 aacaagaacc accattcact gagtagctaa tgggtttggg gcctgggaca ttccatctga
     6841 ggtccttcct gaacatgtca ctccacagca gaggaccggt tgcagcttac ccagaaccac
     6901 tcctccagga gagctggatg ttttgcgtgc aacaccttga gcactgactg ctattgttca
     6961 aaaaaagcct ttgctgcatt cggaggactg ccccgtgccc tgaggtgact tcctaactat
     7021 gtggtttcat tagcgaattt attttttgtg ctgggtggac atttgtattt tgttaggttg
     7081 ctgtttaagc tcaagtttgc tgtgctctct gcagctacaa aacatcttgg catatttaag
     7141 agtggctttt ataaatagct ttattctgat attaatcaga ttcccaactt tactgagaat
     7201 taaggactgg ggtactttaa agaaatgcaa atagcaattg aagaaccact gctgcaggtg
     7261 gtagccctgg ctagactgaa ttacactaga aatcagccag aaggaagcgt ccttgggatc
     7321 ccagatcact cttttttttt ttttttaaaa ggggcagccc cttgatggct catctctctg
     7381 aataacagtt acgtcttcat atcgatacca gatgccttct tcatcatgcc actgaagcca
     7441 ctcaccacct tcaagaacat gccaacctct gtcagattca cttacccaca aacaaggagg
     7501 cacgtttggc acaaagtgtt gtcctccagg tccaagtgga ctctacagag tgcttgacct
     7561 caacacactg gattccaggt ggactggacc aagagcaggc aaagacacgg gaactgaaaa
     7621 actccacagg gtttggagaa tagaaatgaa aagccacgtc atataactca agaataaatg
     7681 gtgttttgga aattttaaaa ttatcatcga aggtggtgaa actatttcag gcccaaatga
     7741 aaggaaatcg ccagttgggg atgaaatcac agagcctgtg ttttatgata tggttggatg
     7801 tccactgatg aaattttaaa ggagtttcag ttttaaaagt gcgcatgatt ctacatatga
     7861 gaattcttta ggccaagaaa ctgtccttgg ctcagaggtg ttgggaatta aagcagagag
     7921 aagccattcg tgatgcttag aaccaaggat ggtcatgtac acaaagacca tcgagacggc
     7981 cattcttgtt tacaaaacac ttaccaagaa agcactttgt aggggaactt tagtaagttc
     8041 ttctcatttc attatgtttc ttccaaggaa acaggagaga ctgaattaat aattctctct
     8101 ttcctcttaa gcacttttaa aataataaag tacatcttga aatttgggga ggcatctctg
     8161 atttaaaaaa agaaaaaggc tgcttgatgt atgttatgca gagacactct gcctctggtg
     8221 gctgcagagc aatacccaag cctcatttgg aaggctcaac atttggaatt gcactttaat
     8281 tgattaatcc tcaattcatg tggccttacg ggatggtggg tctgggaccc caattcattc
     8341 ttatctgcca aagaattatc tagaagcaca tcaaatacca gcaccccacc tgctcaatgg
     8401 gggtggaaaa cttttgtatc cctaagcata ttattttata gtgtctgcca tgccatgtgg
     8461 aaatacttta tttttaacct caggatttaa ataaagtaaa cactatgaca ttta
//
