LOCUS       NM_006581              12793 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens fucosyltransferase 9 (FUT9), mRNA.
ACCESSION   NM_006581
VERSION     NM_006581.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Xu,A., Sun,M., Li,Z., Chu,Y., Fang,K., Zhang,Y., Lian,J., Zhang,L.,
            Chen,T. and Xu,M.
  TITLE     ELF4 contributes to esophageal squamous cell carcinoma growth and
            metastasis by augmenting cancer stemness via FUT9
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 56 (1), 129-139 (2024)
   PUBMED   37674363
  REMARK    GeneRIF: ELF4 contributes to esophageal squamous cell carcinoma
            growth and metastasis by augmenting cancer stemness via FUT9.
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Kadirvelraj,R., Boruah,B.M., Wang,S., Chapla,D., Huang,C.,
            Ramiah,A., Hudson,K.L., Prudden,A.R., Boons,G.J., Withers,S.G.,
            Wood,Z.A. and Moremen,K.W.
  TITLE     Structural basis for Lewis antigen synthesis by the
            alpha1,3-fucosyltransferase FUT9
  JOURNAL   Nat Chem Biol 19 (8), 1022-1030 (2023)
   PUBMED   37202521
REFERENCE   3  (bases 1 to 12793)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 12793)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 12793)
  AUTHORS   Mondal,N., Dykstra,B., Lee,J., Ashline,D.J., Reinhold,V.N.,
            Rossi,D.J. and Sackstein,R.
  TITLE     Distinct human alpha(1,3)-fucosyltransferases drive Lewis-X/sialyl
            Lewis-X assembly in human cells
  JOURNAL   J Biol Chem 293 (19), 7300-7314 (2018)
   PUBMED   29593094
REFERENCE   6  (bases 1 to 12793)
  AUTHORS   Nakayama,F., Nishihara,S., Iwasaki,H., Kudo,T., Okubo,R.,
            Kaneko,M., Nakamura,M., Karube,M., Sasaki,K. and Narimatsu,H.
  TITLE     CD15 expression in mature granulocytes is determined by alpha
            1,3-fucosyltransferase IX, but in promyelocytes and monocytes by
            alpha 1,3-fucosyltransferase IV
  JOURNAL   J Biol Chem 276 (19), 16100-16106 (2001)
   PUBMED   11278338
REFERENCE   7  (bases 1 to 12793)
  AUTHORS   Cailleau-Thomas,A., Coullin,P., Candelier,J.J., Balanzino,L.,
            Mennesson,B., Oriol,R. and Mollicone,R.
  TITLE     FUT4 and FUT9 genes are expressed early in human embryogenesis
  JOURNAL   Glycobiology 10 (8), 789-802 (2000)
   PUBMED   10929005
REFERENCE   8  (bases 1 to 12793)
  AUTHORS   Nishihara,S., Iwasaki,H., Kaneko,M., Tawada,A., Ito,M. and
            Narimatsu,H.
  TITLE     Alpha1,3-fucosyltransferase 9 (FUT9; Fuc-TIX) preferentially
            fucosylates the distal GlcNAc residue of polylactosamine chain
            while the other four alpha1,3FUT members preferentially fucosylate
            the inner GlcNAc residue
  JOURNAL   FEBS Lett 462 (3), 289-294 (1999)
   PUBMED   10622713
REFERENCE   9  (bases 1 to 12793)
  AUTHORS   Kaneko,M., Kudo,T., Iwasaki,H., Ikehara,Y., Nishihara,S.,
            Nakagawa,S., Sasaki,K., Shiina,T., Inoko,H., Saitou,N. and
            Narimatsu,H.
  TITLE     Alpha1,3-fucosyltransferase IX (Fuc-TIX) is very highly conserved
            between human and mouse; molecular cloning, characterization and
            tissue distribution of human Fuc-TIX
  JOURNAL   FEBS Lett 452 (3), 237-242 (1999)
   PUBMED   10386598
REFERENCE   10 (bases 1 to 12793)
  AUTHORS   Kaneko,M., Kudo,T., Iwasaki,H., Shiina,T., Inoko,H., Kozaki,T.,
            Saitou,N. and Narimatsu,H.
  TITLE     Assignment of the human alpha 1,3-fucosyltransferase IX gene (FUT9)
            to chromosome band 6q16 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 86 (3-4), 329-330 (1999)
   PUBMED   10575236
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CK904220.1, BC036101.1,
            AL512406.14 and CK299829.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006581.3.
            
            Summary: The protein encoded by this gene belongs to the
            glycosyltransferase family. It is localized to the golgi, and
            catalyzes the last step in the biosynthesis of Lewis X (LeX)
            antigen, the addition of a fucose to precursor polysaccharides.
            This protein is one of the few fucosyltransferases that synthesizes
            the LeX oligosaccharide (CD15) expressed in the organ buds
            progressing in mesenchyma during embryogenesis. It is also
            responsible for the expression of CD15 in mature granulocytes. A
            common haplotype of this gene has also been associated with
            susceptibility to placental malaria infection. [provided by RefSeq,
            Nov 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK314966.1, SRR1660809.26387.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302103.6/ ENSP00000302599.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-523               CK904220.1         6-528
            524-1044            BC036101.1         506-1026
            1045-12220          AL512406.14        36267-47442
            12221-12793         CK299829.1         18-590              c
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.1"
     gene            1..12793
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /note="fucosyltransferase 9"
                     /db_xref="GeneID:10690"
                     /db_xref="HGNC:HGNC:4020"
                     /db_xref="MIM:606865"
     exon            1..239
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /inference="alignment:Splign:2.1.0"
     exon            240..328
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /inference="alignment:Splign:2.1.0"
     exon            329..12793
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /inference="alignment:Splign:2.1.0"
     CDS             337..1416
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /EC_number="2.4.1.152"
                     /note="fucT-IX; fucosyltransferase IX; galactoside
                     3-L-fucosyltransferase; alpha-(1,3)-fucosyltransferase 9;
                     fucosyltransferase 9 (alpha (1,3) fucosyltransferase)"
                     /codon_start=1
                     /product="4-galactosyl-N-acetylglucosaminide
                     3-alpha-L-fucosyltransferase 9"
                     /protein_id="NP_006572.2"
                     /db_xref="CCDS:CCDS5033.1"
                     /db_xref="GeneID:10690"
                     /db_xref="HGNC:HGNC:4020"
                     /db_xref="MIM:606865"
                     /translation="MTSTSKGILRPFLIVCIILGCFMACLLIYIKPTNSWIFSPMESA
                     SSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNK
                     SHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRR
                     DSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTY
                     GQAFGEYVNDKNLIPTISTCKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSREN
                     YENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHA
                     CLACDHVKRHQEYKSVGNLEKWFWN"
     regulatory      12769..12774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /note="hexamer: AATAAA"
     polyA_site      12793
                     /gene="FUT9"
                     /gene_synonym="Fuc-TIX"
                     /note="major polyA site"
ORIGIN      
        1 agtagctgta gcagcttcag cgaagccgga gatgggcaga gagcgcgcgc ggcgcagcag
       61 ctccagattc actgctctcc cctgcagctc cccgcgcccc cgccgctgtc gctgcctcgg
      121 tgtcccccag ccccagtcgc gctcttagga cagcgccgcc accgccgcct ggccctgcct
      181 gcctcctgcg ccgcgcagcc ctcgcgagcg ccccggatgg cgctttaccc ctaggaccga
      241 tttagaatgt aataactcaa ggatttgata atacagtgaa gtagtataac aactgtctac
      301 gtgcttccca tgatatgttc tctatattga aaaattatga catcaacatc caaaggaatt
      361 cttcgcccat ttttaattgt ctgcattatc ctgggctgtt tcatggcatg tcttctcatt
      421 tacatcaaac ctaccaacag ctggatcttc agtccaatgg aatcagccag ctctgtgctg
      481 aaaatgaaaa acttcttttc caccaaaact gattatttta atgaaactac tattctggtg
      541 tgggtgtggc catttgggca gacctttgac cttacatcct gccaagcaat gttcaacatc
      601 caaggatgcc atctcacaac ggaccgttca ctgtacaaca aatcccatgc agttctgatc
      661 catcaccgag acatcagttg ggatctgaca aatttacctc agcaagctag gccacccttc
      721 cagaaatgga tttggatgaa tttggaatca ccaactcaca ctccccaaaa gagtggcatt
      781 gagcacttgt ttaacctgac tctgacttac cgccgtgatt cagatatcca agtgccttat
      841 ggcttcttga cggtaagcac aaatcccttc gtgtttgaag tgccaagcaa agagaaattg
      901 gtgtgctggg ttgtgagtaa ctggaaccct gagcatgcca gagtcaagta ttacaatgag
      961 ctaagcaaaa gcattgaaat ccatacctac gggcaagcat ttggagaata tgtcaatgat
     1021 aaaaatttga ttcctaccat atctacttgt aaattttatc tttcctttga aaattcaatc
     1081 cacaaggatt acatcacgga aaagctatac aatgcttttc tggctggctc tgtacctgtt
     1141 gttctgggac catctaggga aaactatgag aattatattc cagcagattc attcattcat
     1201 gtggaagatt ataactctcc cagtgagcta gcaaagtatc tgaaggaagt cgacaaaaac
     1261 aataagttat accttagtta ctttaactgg aggaaggatt tcactgtaaa tcttccacga
     1321 ttttgggaat cacatgcatg tttggcttgc gatcatgtga aaaggcatca agaatataag
     1381 tctgttggta atttagagaa atggttttgg aattaaaatt tttcatcact tgcacacttg
     1441 ataaatattt tgatgagata tcatccaagt attgaggata agaagagatg caacatacta
     1501 cttttgtgtc acaatttatt tttatcaccc tctctagggt aacgtgtata ttttggtgga
     1561 gatttttaaa agctcagcat gagcaatcat tccattcggt tttaaattat cctgtatata
     1621 cctaattatg tgcactggag agtaatttat tcttcattat catttgtaaa cattgttttt
     1681 tcacattttt gtagttgtcc ataatgtaag cttgtggttt gattattgtt tccacactga
     1741 tcagctgttt aatctatttg ggaaatgaag atgcacatct taaagtatga aaaattttca
     1801 ctaagtatta caatgtctag ttccaacttt gcatactata acagaggaag aacatgttgc
     1861 gattgaattc taacctcttt gactccgaag atgaatgaag tgtataactg tctctatttg
     1921 atctattttt tttacctgtt tatcacattt gtgaaggtga aattattcat ggagtgaata
     1981 agaaagatat gaagcagaac tgttctattc gggaagctat tagacttctc atttattttc
     2041 attaagctga tttgcagcta cttattctca tggtcttaaa ttaaattatt caagtatttt
     2101 taaatatcca atttgttgtg attttcagca cctgggaagt aatcccaata atactttaga
     2161 aaatctaaga cagttctttc tgctactgat gacactcatt gtcataataa aacaaataat
     2221 ttcctcaaat aacaaagaaa aatgatacct ataaatatat ttataaatgg tgtcatttat
     2281 gaacaatgtt taattatgta tcaatttaag atttttttct gaagccctaa tatttaaaat
     2341 ggtcttattt taccatatgg atataagatt tggctcataa tgatgagccc tatcatttga
     2401 tttgagttct atcatttaag agagcctaaa taaaattatc atcaaggtat taaatataag
     2461 acgttaaata taataaagtg gggatatata gaaaacacac agtgttagca cacagtaaga
     2521 tctcaatgca catttgttgg atgaataaat aaatgcaatt gaattcccag aaaaatgatt
     2581 gtttcaagga agtgacagtt ctactttaga agtactaatt ggagatgact tttatatccc
     2641 attttggtaa ttattcatac atagcacata tgaccatgat gttcagggct ttatagaacc
     2701 aaataaacct accattacat gaaaattttg aagagtatat tcctgaactt gcagctgcct
     2761 atagcattct cctccataag gctagaggag agctggatat aagcagagat cgtaggtgaa
     2821 aaaagatggg ccattaagga ttcttcaagg aaaacattcc tcaaaatgat ctttagtgcc
     2881 tttattcctt tcaggccaat aatccaccca aaaattagaa tgttctgaga catccatttg
     2941 ggtctatttg tgacttattc gtattacaaa ttgacatcag caaaattcaa aaagaattca
     3001 gtaaaaaggg catagatcca gctatccaga ctatcttgtg agagaataag ctcaccccag
     3061 caccagaaag atttgagcag aggaatattc ctgtaatgaa tagaggattg tttgattctc
     3121 aggtatattt cttaagccaa gattgaatgc agtgtcaggc actgccttaa cacccacaga
     3181 gataagatga acaaggtact atcactgcct aaaatgagct tacaattaag tggatagaca
     3241 tataaaagct tcctttcaat tcagcctgag actgtttata tgcatgtgct actccgaatg
     3301 acgtttgtat gggtcaatgc tgatttaatg gggaaaaaaa gtagaaaatt tccccaattg
     3361 cttagctgtt tgacatgttt ctgagctgca aactttgtca actcttttga cccaacatat
     3421 tattctctcc gtattatctc ttctaaccac aatgcattaa gaaataatat ttttatgtgg
     3481 atgtcctcct caataagtaa agatctccat ccatatttaa ttaattggta agacagtcaa
     3541 attcatacat ttacatttac tttgtcaaat tttttggtct ataaaaagca tagagatcta
     3601 aagaggatac actgctaaca agtttagaaa gcacctgctt ttttttatta ttattatttt
     3661 tgtgatagag tcttgctctg tcgcccaggc cggagtgcag tggtgcagtc tcggctcact
     3721 gcaacctcgg cctcccaggt tcaagtgatt ctcctgtatc agcctcccga gtagctagga
     3781 ttacaggcgt gtaccactat gcctggctag tttttgtatt tttagtagag atgggtttca
     3841 ctgtgttggc caggctggtc ttgaactcct gacctcaaat gatccacttg cctcagcctc
     3901 ccaaagtgct gggattacag gtatgagcca ctgtgtctgg cccacctgtt ttttttgaag
     3961 gcagaaaaac attcaatatt aaaagcattc aatattattt caacctgcct tggctctagt
     4021 gaatgttcat gatttgaaag aactgaaaga cctaaaggca gtgtctctat tgttgactac
     4081 aaatgtaggc tctttgttgg actatgacta taatcacaca ctgataatat gacgcaagta
     4141 gaggcagtac tactaagtat ctagcaggtg ctttgaaacc ccattactaa gtcccagaag
     4201 actgtttcct aaatttgaag tggaagaatt aaatgactca atggaaccct ttagctgtgg
     4261 gtccattgat gtgtgagttt tagagctgga aggaattata attttgttgt ttataattgc
     4321 tataatatag aaattataat tgcttttctt tctacttatg agttaactag atccagagat
     4381 atttattcaa gacacacatt cacacacaca tacacacaca cacacacaca ccccacagct
     4441 agtgacaata gttaagattt ggatttaagg atcctatctt tctttatccc actttctttt
     4501 caatcgcatt cataacttat tatagaatta tgtctcaatt tttacaaatg tgaatgtctt
     4561 attggtattt ttagggccta tagaatggta cacctcatgt agaagaactt cgacttttga
     4621 aggtatacaa taaataaaac atcaaacttg ttagcattcg ttctggttta aatattttag
     4681 gagttgagtt cagcagaagg aaatgggcta tcactacatt taagtgaaat acaaccctca
     4741 agaaagtgta tggttcacag ccactccttt aataaatcct gtgggttgca attctttgta
     4801 agaaactcat gaaaaagata tattgattca acaaaattgg tttgctgttg attatacaaa
     4861 ccaataatta tgcctagaat taaattgtgg acacaaaaaa gggaacagac catttaaaat
     4921 gttttatttc aaaatatcga aaagctatta ccttcaatac tctaaatatt taaatattaa
     4981 ttattgtaga catcagtaag aataaataaa tgttaatcat tatagacctt actaataaga
     5041 atatatactg aattgtccac atccagagta ttacaccctg aacaagaaag acatgagagc
     5101 ctgacagtca tatatattat gacagctgta tatctgacaa tatgaagtag aaactgaaaa
     5161 aaaaaatcat cttagtggga aacaaataca tgcttaaaat tgttatattt actgtaaaac
     5221 tcctgataat cttagattgt gcagaaatgt ttcttatttt gaggggctta gaaagctcag
     5281 cgcatttgat gtataaagca acagaatatt taggaattat ttgcggaggc tttaaattct
     5341 tacctccaat tggaggttaa atattaaaag aataggaaac tatgcctctg atattttttt
     5401 tgtcagccta tttttaaact agattccatg agtcttaagg taatatccta gaattttttt
     5461 tttgcgtgaa ttaaagttac cacaaatttc aggtccttac cttatgactg tgtttgatca
     5521 tatctttgag ttcccttgaa ctctgatctc attttgagaa acattttgag aaacttctta
     5581 acaattacaa tcgagctgtt aaatataaca aacatcagaa tctaaaggat aagcacagtg
     5641 cctaatgtac aggagggtca tatcaaatac ttgttgaata aatacatgaa tgtgataagt
     5701 ggtcctgtaa aatatttttt aagagtagtg acaatgttta ttggtaccaa ttggagatga
     5761 tattttatat ttattttgtt agtttgtcac ctggatgcaa aaggaaggca ttttaattcc
     5821 tttctatctt acaacattgt aaagcatcaa aagaagtaac atctcaagaa agattagaaa
     5881 ggatttggat cacgttaaca ttctatgcaa atattagtaa catatcaact gtttctaaaa
     5941 ggaaagaaaa ttagtattta aggagagtat aaagtgcgag cactgtgaag ggcagatatc
     6001 tattaccaaa ccacatggag agaaatatag tctctctcct aacttcattc cattgtattg
     6061 caatcagtca attaaggtga tttttttttt ctggatgtct caatctagag attttgtagc
     6121 attttgcaaa tgggtatgct tcactactat ccacagtgtt ctccccttta gtaattcata
     6181 ttgaatatat ccatattcat atgcatttta taaaacagtt aacagaatct aaataagata
     6241 tagccggcta aatctggaat gtgagtgcta ccctaggtta ttagttcaac aaactaattt
     6301 tgagagagcc acttaacctc tctggacctt gtttgtaagc tttcttctag cactattact
     6361 cttatctaag atcgaaattg atatatgcat gaaattccta atggaatttc aatgtccatc
     6421 tcaaagtaat tttttaggat attccataca gatttccttc tcatttctct gtgtagatat
     6481 ttaaagtatg aaattgatta tttaaactaa tatgtggaaa gaacctaatc cagagcttgg
     6541 aatatagaaa gtgctcaatt aactgacatt cctcctgtat ttcctccctc cccttagttg
     6601 tctatgactt ttgaactcac tgctcactga catcttaatt gcctgcagaa aaatcaatca
     6661 tattttaggc attattttga aatattttag agttttctgt actattcaaa ctcttattac
     6721 aaaaatctaa agatatttat tgatgcaaaa aatttaggga gtcattggaa atacctgagg
     6781 caatcccagg ctaacaataa aatagcataa ttttaagtaa gctagattga gttcatttga
     6841 acatttagtt attttttagc tcatagttaa catatgtcca tgcatttgat ttactttaaa
     6901 aatattttat ttaaaaataa cctctcaaac accagttgct ttctgtcagt tttactcaaa
     6961 gcatgcttcc taggtcacct gacctagaat caccagaagt gttaacaata ccgatttctg
     7021 ggctttgagc catgaaatta gaatttcttc aaataatcca tgcccccccg tcacccaaaa
     7081 aaagagcatg cttttaatac atttccaaaa ttatcctgtt tctgctataa ctggtgaacc
     7141 atcctgtcct aaactagcag ttctcaaaca tgcatatgcc tttgaaactt cctagatcat
     7201 ctacataaaa atcatttgaa atattgttaa taatacacag cttctgaatc tctgggtgtg
     7261 agatgggacc aggaatctta ttttaaaaat ctccccagat ggttctggtg tcctgctagt
     7321 tttgaggttt tcatcccacc tgtttagatg ggtagtattc tattaaatat gaggtattac
     7381 ctttctttag ttcacctgga atcttcttaa atatatcggt aaagtattct acttcaaaat
     7441 ccaagacatt ttgtatacct aattatttca cacaagagcg tggaactcac cagtcttgtg
     7501 acttggggca ggttatttat agtctccaga cactttttcc agttgcgagt tgcttatagt
     7561 agttcgcgaa cctgaatgca cattagaacc atctggagag gctttaaaat tgcagatgtc
     7621 caaaccccac ctccagagag ctgatctatt tagtttgagg aagagcccag aagtctaaca
     7681 ggtgtaaatg ttccacaggt aatactaatg caggcatacc tcaggaaatc accagttctc
     7741 gtcttggagg aactattagg aaaataaaag attatatatc ttgaaaaaaa tctctaattt
     7801 gctttctagg ttctaactgc atgtttatat tttgcaacag gaaagactgg tctagacaat
     7861 tactaggata tatgcttctg tgatctgctt ttctattgta tgtgtttaag agaccacatt
     7921 cttgggttcc aaagtctcct tgtctgatag gtaatttgca attgagatat ggcaaagaat
     7981 tgtagcatcc aaatttttga caactacaga tttagaattg aatccaaggt ctgccattca
     8041 ctagctatgt gcaagttact caacttctct tatcctcaga ttttgcagct gtaaagcaga
     8101 ggtagcatat ttctgagaaa ttatattcag gtgactagat atgtgttcaa gtgttatctt
     8161 ttattcttat aaaagataat tgtgaggaat ccagtgctat tttcacaatt ttaattctaa
     8221 aactctgaag acattaaaca ggcagtttgt aggaatcatt gaaattttat acaagccgaa
     8281 ggaacaacat tcagcaaaac ggaaacaaat aaatacagca tggaagcata tattcatgtt
     8341 tcccataatt actaagttac ggaaggggtg tgaggagctg gcagtggtgg tgaataggca
     8401 ttgtttgcat tactaaatat atataccatt tcttaagata attggaactc aagcagtcct
     8461 aattttctct ccctgttatg agcaatcagt aaatttgtag tcaatttaac ttattatcag
     8521 ttggtatagc gcatgtcaca gaataggata gcaagaagat tctcagagat cattttctgg
     8581 taaagaaaaa ggcctagcaa tatttaacag tttgacttaa tccatcactt cagctggtcc
     8641 actagttttg acacaggact agttaatgat ttgttaggaa gtctccatct actcttaaac
     8701 taatttaaaa aaaagctaaa agttgctttt aatatgttag ggtataactt caataaacag
     8761 gagtatttat ctagtggttc agaaataaaa taattgttag ccagcctgtg attaaggcta
     8821 ccataattgc ataataaata gctttgacgt gaataatctc agttgttcaa gtgtacaaat
     8881 ttaaagctgt actatgttag aataaaaaag taggctcaag gaaaatttct atttttctat
     8941 atgaaaaaga gaatgatatt caaaatagta tttttaagta gtttctaatt gtgttccttt
     9001 aaagagtatt agaaatgtct gttatgtcag taacattaga aaacttcaaa aactgattta
     9061 tttttctgag ttctctggaa accaaacaat tttgaattag ttgtgtaagt aaagtaagtt
     9121 aagttatagc ttgctggaat tttattgtgg aaaacaaata tagattttat ctgaaaaatt
     9181 ttaagggcca attctacaga aagttatgct aacatgctaa ctttccacac atggctgcat
     9241 tccattttga ttaatgaggg ttcaggaaat agaaaggcag tctctgcaga agcagtatcc
     9301 aaaggctaaa ttaacaaccc aggactacca ctcattatgt gagaaatatg ggccagagtt
     9361 acaatatcac atctccagtc tcagattggc ctcattagcc accttcagtt ccttaaatga
     9421 gataatcctg aagatgccat ccttgtccaa ggtgagattg ccagaggata tgagctcatc
     9481 taggatggaa tacatgtctc cagggactgt aatgagatca ggcttttcat ttactggttt
     9541 tctgcctcaa gagtccttaa gcaaatgaag attatctgat tgtctatgta aacctggaag
     9601 tagtctactt tttagataaa agataatcta tcttgaatat ttcatatgtg attttaaaat
     9661 aattaatcaa aaaacaaaga ctatcatatt tgagaacaag attttactta gaagggaaaa
     9721 aaaagaactt tcagcttaat cggggttaag ggagtaattg tgacaatata aaatggcatt
     9781 gttatagaat ccctaaaagg taaataatgt atgagatgaa ggtaggaagt aatgtgtgct
     9841 tgcagaaatg caactggtca aaattacttg aatgaaattg ttgaggttaa acatcttcat
     9901 tataataata tgtcttattc tgattccaag acaatacgaa tgtagagaag agatttttaa
     9961 atggaaaaag taaggagcta acaagtctgt acctagattc aagttcacaa agcggcaaaa
    10021 aggagaaaag tgtcaacaag aaacaagaag aggagtagta aactaaggat caaagcccaa
    10081 gttccagttt tcacaatctg ctgtataatt cactaacatt tctgagccat agatttttaa
    10141 tctgtaaaag gagtgtgatg gactgtacat tagaagtacc tgagaagctc ttaataaatc
    10201 ttgatgaatt ggttagaaat tctaatttaa ttagcctggc acagtgcaca aactgccata
    10261 tattcgtgca gatgttttct tcttttctta ctgcgcattt ccatgtggcc atgtcactac
    10321 aaggggtggg gaagaagtta atgtgtaatt gcttcaggag ctctgaataa aaggttgctg
    10381 aagcaaaata ctgcaatgaa gttctgaaaa tgaagagtta ggactttaca tgatttctct
    10441 tgcctcattc cctcaagaat gcacatatag acacacatat gccaatatct tttttatgta
    10501 gttcaagtat aactttgaat aaaatgactt acattcaagg aaggtcaaaa ctattattct
    10561 gaaacaaaat gtgacaattc tagccattat gtgaattgta agggtcagta atccataaat
    10621 tatttggatg gtaattgtat gttttattaa tgtgatgtaa ttttgcacta ttgtattgtc
    10681 atatttaatc aaactcttaa aaataccctg aaacataatg ccaaatgtac agtttatagg
    10741 ttcgtgttat ttctgataag aatttaattg cattgtgcta atagttgttt attgctatta
    10801 actttctgtg tatgtattat tgcgatgcag acccaatagg gttattttaa gcacactaat
    10861 tagtcatctg gatttttata gataaaatca catgttgatc agaaatcatt tttctactta
    10921 tttttttgta gagtattttt ccacacttga aaaaaacatg attattttat atcaaacatc
    10981 aacttttctt taaatcttat tttagtagct aatgcatgtt tgaaataaga tgtgttagaa
    11041 attatatgac tatattaagg ttgtgtttat attaggtgaa aaattgcatg caattggtga
    11101 atcatgcagc tttggggaca tttacactgc cactcacaca caggctctgt ctcaatccgt
    11161 atgcatgatt cacgtccttt caaaagtccc ttttcaacat ttggtaaagt gaaaatgtta
    11221 ttcatagtgc taatgagcca ataaacaatg ctgcgtttct tttttctaac tgcagcatat
    11281 tgtggtaaat tttctcccaa aactttattt ttggagttat tgttggcaat aaatacaaca
    11341 aattatttgt tggcaagtga tacaatttac tgtataacaa cttaatagta caaagcatga
    11401 agatgtgaaa atatcatctt aaccagtttc attctatgaa cataatattc tggcagcttt
    11461 ttctataact gagaatggta tatcttttta tacactgcct aaatcagtac tactgccagt
    11521 cacctgaggt caggtctgca caacactaaa ttgggcaata acatagaaca tctaggcagt
    11581 cttgacagtc aaccagtgta atcactaggg gaagaaaaag taggcctacc cttttactta
    11641 ttaacttaag taataaaaat tgtataaaaa tatgaatgtt ggctgcagag gagcctttac
    11701 atgcagataa tttgaagcag tctttgaaaa taacaaaaat tattccattt aatgaagggt
    11761 ttgttttgtt tagcttttct cttttattca gaaaacatac ctgtgctttt gaaagggctt
    11821 aatcccaaac aggtaatatg tgtggatcaa tcatctctcc tcccatgaaa ttaatcattc
    11881 atggtaatat attaaggctg gaacgtagct cttagtgact taaaacatga cagtaagcat
    11941 ttacactgtt ggaaggtaat ttcattgcta tgttattaaa atgatgggaa tcctatttat
    12001 acatttattt atttatttat ttacagaaga ttggttcctt ccagttcaat ttaacagctt
    12061 cagtgaagtt agtataatga taagaaaaat tgactgtagc tattattcca agtgaaaatc
    12121 atgcagctga gtcctgctgc atcctgggag caaagcatta attcaaatga ggagtagtca
    12181 gtcctagcac tgtagacgcc gactttacca accaagatag tgtatgtgtg tgacattcag
    12241 ctaacattga tctaggcact tagtttgcta ccacattgtt cccttcattg attgaaactg
    12301 taaataacat aacactttaa ggcagctaag caaatatttt aataagccat gaaaggcaag
    12361 atgccagaga aaatctgtat attcagctat ttggagaact cgtgttttcc acaaattaaa
    12421 ctggagatgt catttgaaat tttcttccct taaacatgct gtcacaacat ggattccttc
    12481 tcatggatgt ctttctaggc ttataaatat atggtgtgat tgctataatt ttgtgaaatt
    12541 ttattcagca attaatagtg atttcagcaa tatgtactaa gattccaagg cagaaataaa
    12601 tgtataaagg atttgagcct gtatgtgtaa gaagaaactc tctcttcagt catatttcct
    12661 aaattcagtg taagtacctc gctgatttag cactggagtt attccttgaa tgtgtaaata
    12721 atgatgttct attctgacct aatgaattcc tgtaatgtga atatttaaaa taaaagaatt
    12781 caatttaaat gta
//
