LOCUS       NM_001371533            4249 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens fucosyltransferase 8 (FUT8), transcript variant 2,
            mRNA.
ACCESSION   NM_001371533 NR_038170
VERSION     NM_001371533.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Lopez-Cortes,R., Muinelo-Romay,L., Fernandez-Briera,A. and Gil
            Martin,E.
  TITLE     High-Throughput Mass Spectrometry Analysis of N-Glycans and Protein
            Markers after FUT8 Knockdown in the Syngeneic SW480/SW620
            Colorectal Cancer Cell Model
  JOURNAL   J Proteome Res 23 (4), 1379-1398 (2024)
   PUBMED   38507902
  REMARK    GeneRIF: High-Throughput Mass Spectrometry Analysis of N-Glycans
            and Protein Markers after FUT8 Knockdown in the Syngeneic
            SW480/SW620 Colorectal Cancer Cell Model.
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Tomida,S., Nagae,M. and Kizuka,Y.
  TITLE     Distinctive domains and activity regulation of core fucosylation
            enzyme FUT8
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (4), 130561 (2024)
   PUBMED   38218458
  REMARK    GeneRIF: Distinctive domains and activity regulation of core
            fucosylation enzyme FUT8.
            Review article
            Erratum:[Biochim Biophys Acta Gen Subj. 2024 Jun;1868(6):130615.
            doi: 10.1016/j.bbagen.2024.130615.. PMID: 38614919]
REFERENCE   3  (bases 1 to 4249)
  AUTHORS   Shi,M., Nan,X.R. and Liu,B.Q.
  TITLE     The Multifaceted Role of FUT8 in Tumorigenesis: From Pathways to
            Potential Clinical Applications
  JOURNAL   Int J Mol Sci 25 (2), 1068 (2024)
   PUBMED   38256141
  REMARK    GeneRIF: The Multifaceted Role of FUT8 in Tumorigenesis: From
            Pathways to Potential Clinical Applications.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4249)
  AUTHORS   van Pul,L., Maurer,I., Boeser-Nunnink,B.D.M., Harskamp,A.M., van
            Dort,K.A. and Kootstra,N.A.
  TITLE     A genetic variation in fucosyltransferase 8 accelerates HIV-1
            disease progression indicating a role for N-glycan fucosylation
  JOURNAL   AIDS 37 (13), 1959-1969 (2023)
   PUBMED   37598360
  REMARK    GeneRIF: A genetic variation in fucosyltransferase 8 accelerates
            HIV-1 disease progression indicating a role for N-glycan
            fucosylation.
REFERENCE   5  (bases 1 to 4249)
  AUTHORS   Martinez-Duncker,I., Michalski,J.C., Bauvy,C., Candelier,J.J.,
            Mennesson,B., Codogno,P., Oriol,R. and Mollicone,R.
  TITLE     Activity and tissue distribution of splice variants of
            alpha6-fucosyltransferase in human embryogenesis
  JOURNAL   Glycobiology 14 (1), 13-25 (2004)
   PUBMED   14514715
  REMARK    GeneRIF: Expression of FUT8 is regulated by three different
            promoters, starting transcription in exons A, B, or C.
REFERENCE   6  (bases 1 to 4249)
  AUTHORS   Takahashi,T., Ikeda,Y., Tateishi,A., Yamaguchi,Y., Ishikawa,M. and
            Taniguchi,N.
  TITLE     A sequence motif involved in the donor substrate binding by
            alpha1,6-fucosyltransferase: the role of the conserved arginine
            residues
  JOURNAL   Glycobiology 10 (5), 503-510 (2000)
   PUBMED   10764839
REFERENCE   7  (bases 1 to 4249)
  AUTHORS   Miyoshi,E., Noda,K., Yamaguchi,Y., Inoue,S., Ikeda,Y., Wang,W.,
            Ko,J.H., Uozumi,N., Li,W. and Taniguchi,N.
  TITLE     The alpha1-6-fucosyltransferase gene and its biological
            significance
  JOURNAL   Biochim Biophys Acta 1473 (1), 9-20 (1999)
   PUBMED   10580126
  REMARK    Review article
REFERENCE   8  (bases 1 to 4249)
  AUTHORS   Yamaguchi,Y., Fujii,J., Inoue,S., Uozumi,N., Yanagidani,S.,
            Ikeda,Y., Egashira,M., Miyoshi,O., Niikawa,N. and Taniguchi,N.
  TITLE     Mapping of the alpha-1,6-fucosyltransferase gene, FUT8, to human
            chromosome 14q24.3
  JOURNAL   Cytogenet Cell Genet 84 (1-2), 58-60 (1999)
   PUBMED   10343104
REFERENCE   9  (bases 1 to 4249)
  AUTHORS   Costache,M., Apoil,P.A., Cailleau,A., Elmgren,A., Larson,G.,
            Henry,S., Blancher,A., Iordachescu,D., Oriol,R. and Mollicone,R.
  TITLE     Evolution of fucosyltransferase genes in vertebrates
  JOURNAL   J Biol Chem 272 (47), 29721-29728 (1997)
   PUBMED   9368041
REFERENCE   10 (bases 1 to 4249)
  AUTHORS   Yanagidani,S., Uozumi,N., Ihara,Y., Miyoshi,E., Yamaguchi,N. and
            Taniguchi,N.
  TITLE     Purification and cDNA cloning of
            GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase
            (alpha1-6 FucT) from human gastric cancer MKN45 cells
  JOURNAL   J Biochem 121 (3), 626-632 (1997)
   PUBMED   9133635
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB089189.1, Y17978.1,
            AL161871.6, AB049740.2, AF052088.1 and AL109847.5.
            
            On Jul 17, 2019 this sequence version replaced NR_038170.2.
            
            Summary: This gene encodes an enzyme belonging to the family of
            fucosyltransferases. The product of this gene catalyzes the
            transfer of fucose from GDP-fucose to N-linked type complex
            glycopeptides. This enzyme is distinct from other
            fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4
            fucose addition. The expression of this gene may contribute to the
            malignancy of cancer cells and to their invasive and metastatic
            capabilities. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, May 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.135230.1,
                                           SRR1660805.92893.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000673929.1/ ENSP00000501213.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-485               DB089189.1         1-485
            486-974             Y17978.1           392-880
            975-975             AL161871.6         77574-77574         c
            976-2599            Y17978.1           882-2505
            2600-2600           AB049740.2         1659-1659
            2601-3270           AF052088.1         1157-1826
            3271-4249           AL109847.5         146441-147419
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.3"
     gene            1..4249
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /note="fucosyltransferase 8"
                     /db_xref="GeneID:2530"
                     /db_xref="HGNC:HGNC:4019"
                     /db_xref="MIM:602589"
     exon            1..485
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            486..583
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            584..1013
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             811..2538
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /EC_number="2.4.1.68"
                     /note="isoform a is encoded by transcript variant 2;
                     glycoprotein 6-alpha-L-fucosyltransferase;
                     GDP-fucose--glycoprotein fucosyltransferase;
                     GDP-L-Fuc:N-acetyl-beta-D-glucosaminide
                     alpha1,6-fucosyltransferase; alpha1-6FucT;
                     alpha-(1,6)-fucosyltransferase; fucosyltransferase 8
                     (alpha (1,6) fucosyltransferase)"
                     /codon_start=1
                     /product="alpha-(1,6)-fucosyltransferase isoform a"
                     /protein_id="NP_001358462.1"
                     /db_xref="CCDS:CCDS9775.1"
                     /db_xref="GeneID:2530"
                     /db_xref="HGNC:HGNC:4019"
                     /db_xref="MIM:602589"
                     /translation="MRPWTGSWRWIMLILFAWGTLLFYIGGHLVRDNDHPDHSSRELS
                     KILAKLERLKQQNEDLRRMAESLRIPEGPIDQGPAIGRVRVLEEQLVKAKEQIENYKK
                     QTRNGLGKDHEILRRRIENGAKELWFFLQSELKKLKNLEGNELQRHADEFLLDLGHHE
                     RSIMTDLYYLSQTDGAGDWREKEAKDLTELVQRRITYLQNPKDCSKAKKLVCNINKGC
                     GYGCQLHHVVYCFMIAYGTQRTLILESQNWRYATGGWETVFRPVSETCTDRSGISTGH
                     WSGEVKDKNVQVVELPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKY
                     LIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQL
                     LARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGV
                     ILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQI
                     AIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVK
                     YPTYPEAEK"
     exon            1014..1129
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1292
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1407
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1645
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1892
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2069
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2220
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..4249
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3252..3257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /note="hexamer: AATAAA"
     polyA_site      3253
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /note="major polyA site"
     polyA_site      3270
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /note="major polyA site"
     regulatory      4229..4234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
                     /note="hexamer: AATAAA"
     polyA_site      4249
                     /gene="FUT8"
                     /gene_synonym="CDGF; CDGF1"
ORIGIN      
        1 attcgcgcgc gcacgccggc gctggccgag gcttccccgc ctgcgctcgt tgtcagagcc
       61 gctccggcgc gtgcgcgcgt tatctccggc cgacccgagc agccggttcc ctcctctcca
      121 ggccccctcc ccatcccacc cccgccgcct ggccccagcc gacccgtccc ttcgtctccc
      181 cgcggaatgg ggccggcact gctcagggtc gcgcgccctg gacccagctc gctctcggtc
      241 tcgcgctgtc agcgactgcc cggctcgcgc cgcctcgcgc tctgcctcag tcagtggcgc
      301 cgaaggctcc gttaagcggc ggcggcggtt cctgtttccg tttcttcctc tccgttcggt
      361 cgggagtagc atcctccact cagccaccct tcccactccc ccatcgtggg gcagctgcgg
      421 ctgagggctg tggctttggc agctgcgacg gggagcggcg gagaccgcct ctgctcccgc
      481 ctggggttgc tgcttttgct cagaggacat ccatgaccct aatggtcttt ttgttcaaga
      541 taaagtgatt ttttgccttt gttgattaac tggacaaatt cagcatgtag agcgcatgaa
      601 gtacaggaca ataaagcttc ctacacatat caccaggagg atctctttga aagattcact
      661 gcaggactac cagagagaat aatttgtctg aagcatcatg tgttgaaaca acagaagtct
      721 attcacctgt gcactaacta gaaacagagt tacaatgttt tcaattcttt gagctccagg
      781 actccaggga agtgagttga aaatctgaaa atgcggccat ggactggttc ctggcgttgg
      841 attatgctca ttctttttgc ctgggggacc ttgctgtttt atataggtgg tcacttggta
      901 cgagataatg accatcctga tcactctagc cgagaactgt ccaagattct ggcaaagctt
      961 gaacgcttaa aacaacagaa tgaagacttg aggcgaatgg ccgaatctct ccggatacca
     1021 gaaggcccta ttgatcaggg gccagctata ggaagagtac gcgttttaga agagcagctt
     1081 gttaaggcca aagaacagat tgaaaattac aagaaacaga ccagaaatgg tctggggaag
     1141 gatcatgaaa tcctgaggag gaggattgaa aatggagcta aagagctctg gtttttccta
     1201 cagagtgaat tgaagaaatt aaagaactta gaaggaaatg aactccaaag acatgcagat
     1261 gaatttcttt tggatttagg acatcatgaa aggtctataa tgacggatct atactacctc
     1321 agtcagacag atggagcagg tgattggcgg gaaaaagagg ccaaagatct gacagaactg
     1381 gttcagcgga gaataacata tcttcagaat cccaaggact gcagcaaagc caaaaagctg
     1441 gtgtgtaata tcaacaaagg ctgtggctat ggctgtcagc tccatcatgt ggtctactgc
     1501 ttcatgattg catatggcac ccagcgaaca ctcatcttgg aatctcagaa ttggcgctat
     1561 gctactggtg gatgggagac tgtatttagg cctgtaagtg agacatgcac agacagatct
     1621 ggcatctcca ctggacactg gtcaggtgaa gtgaaggaca aaaatgttca agtggtcgag
     1681 cttcccattg tagacagtct tcatccccgt cctccatatt tacccttggc tgtaccagaa
     1741 gacctcgcag atcgacttgt acgagtgcat ggtgaccctg cagtgtggtg ggtgtctcag
     1801 tttgtcaaat acttgatccg cccacagcct tggctagaaa aagaaataga agaagccacc
     1861 aagaagcttg gcttcaaaca tccagttatt ggagtccatg tcagacgcac agacaaagtg
     1921 ggaacagaag ctgccttcca tcccattgaa gagtacatgg tgcatgttga agaacatttt
     1981 cagcttcttg cacgcagaat gcaagtggac aaaaaaagag tgtatttggc cacagatgac
     2041 ccttctttat taaaggaggc aaaaacaaag taccccaatt atgaatttat tagtgataac
     2101 tctatttcct ggtcagctgg actgcacaat cgatacacag aaaattcact tcgtggagtg
     2161 atcctggata tacattttct ctctcaggca gacttcctag tgtgtacttt ttcatcccag
     2221 gtctgtcgag ttgcttatga aattatgcaa acactacatc ctgatgcctc tgcaaacttc
     2281 cattctttag atgacatcta ctattttggg ggccagaatg cccacaatca aattgccatt
     2341 tatgctcacc aaccccgaac tgcagatgaa attcccatgg aacctggaga tatcattggt
     2401 gtggctggaa atcattggga tggctattct aaaggtgtca acaggaaatt gggaaggacg
     2461 ggcctatatc cctcctacaa agttcgagag aagatagaaa cggtcaagta ccccacatat
     2521 cctgaggctg agaaataaag ctcagatgga agagataaac gaccaaactc agttcgacca
     2581 aactcagttc aaaccatttc agccaaactg tagatgaaga gggctctgat ctaacaaaat
     2641 aaggttatat gagtagatac tctcagcacc aagagcagct gggaactgac ataggcttca
     2701 attggtggaa ttcctcttta acaagggctg caatgccctc atacccatgc acagtacaat
     2761 aatgtactca catataacat gcaaacaggt tgttttctac tttgcccctt tcagtatgtc
     2821 cccataagac aaacactgcc atattgtgta atttaagtga cacagacatt ttgtgtgaga
     2881 cttaaaacat ggtgcctata tctgagagac ctgtgtgaac tattgagaag atcggaacag
     2941 ctccttactc tgaggaagtt gattcttatt tgatggtggt attgtgacca ctgaattcac
     3001 tccagtcaac agattcagaa tgagaatgga cgtttggttt ttttttgttt ttgtttttgt
     3061 tttttccttt ataaggttgt ctgttttttt ttttttaaat aattgcatca gttcattgac
     3121 ctcatcatta ataagtgaag aatacatcag aaaataaaat attcactctc cattagaaaa
     3181 ttttgtaaaa caatgccatg aacaaattct ttagtactca atgtttctgg acattctctt
     3241 tgataacaaa aaataaattt taaaaaggaa ttttgtaaag tttctagaat tttatatcat
     3301 tggatgatat gttgatcagc cttatgtgga agaactgtga taaaaagagg agctttttag
     3361 tttttcagct tatttacttt gttttttttc ctgtttctga tatagtaact aattcttaac
     3421 tcagagacat tggtccattt taatactgaa aaccaatttt cattggtaca cattacaaaa
     3481 ttgctaagaa cactgtttgg gagctttcat tctcatattt tggacattgt tttaattgag
     3541 tgaaataatc ataactcctt gctcccagag aagctatcac ctccatttct aaaaccattt
     3601 caggtttgtt tggtagtctt tcttaatgta tttattatcg ctttttgtga gtagggtcct
     3661 gttttatcca ggaaccaatt tctgccctag cctatcatgc cctgcttttg agagtaccag
     3721 gtatctttgg gattgggaag ctggctgttt cagaagtata tgtctcatag tgtgcagaac
     3781 ttggtgacca agtgagaagc gggaccaatg ggagactcac aatggactga gtcttgggat
     3841 tatcttttcc aaattcttcc atgttagaat cacttcagaa aataagactt tgatgctttg
     3901 tctctgagca tcatttttct cctcataaaa acacttcctt attgtatgta gcctgcctcc
     3961 tacagaggcc tggtaggtgt tactgcattc taaaagaaaa atgtcatctc tgtaggagcg
     4021 actatcaggc ctagtgtgaa atattaggga tcctaggcag aagagctatt agtcctggcc
     4081 ttcatatctt caccaaatga aaatactgta tataaaattt caccaccaaa cttaactaaa
     4141 ttctttttct caagtcaagc ctcccaaaga aaaaagaaat taacttccta cagtgtcagc
     4201 aagcaatttt ccatttagtt ttggtacaaa taaaagtcat ttgaaacaa
//
