LOCUS       NM_001382620            4182 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens furin, paired basic amino acid cleaving enzyme
            (FURIN), transcript variant 5, mRNA.
ACCESSION   NM_001382620
VERSION     NM_001382620.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Kubo,Y., Hans,M.B., Nakamura,T. and Hayashi,H.
  TITLE     The Furin Protease Dependence and Antiviral GBP2 Sensitivity of
            Murine Leukemia Virus Infection Are Determined by the Amino Acid
            Sequence at the Envelope Glycoprotein Cleavage Site
  JOURNAL   Int J Mol Sci 25 (18), 9987 (2024)
   PUBMED   39337476
  REMARK    GeneRIF: The Furin Protease Dependence and Antiviral GBP2
            Sensitivity of Murine Leukemia Virus Infection Are Determined by
            the Amino Acid Sequence at the Envelope Glycoprotein Cleavage Site.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Su,W., Ahmad,I., Wu,Y., Tang,L., Khan,I., Ye,B., Liang,J., Li,S.
            and Zheng,Y.H.
  TITLE     Furin Egress from the TGN is Regulated by Membrane-Associated
            RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32
            (USP32) via Nondegradable K33-Polyubiquitination
  JOURNAL   Adv Sci (Weinh) 11 (35), e2403732 (2024)
   PUBMED   39031635
  REMARK    GeneRIF: Furin Egress from the TGN is Regulated by
            Membrane-Associated RING-CH Finger (MARCHF) Proteins and
            Ubiquitin-Specific Protease 32 (USP32) via Nondegradable
            K33-Polyubiquitination.
REFERENCE   3  (bases 1 to 4182)
  AUTHORS   Pandey,R.K., Srivastava,A., Mishra,R.K., Singh,P.P. and Chaubey,G.
  TITLE     Novel genetic association of the Furin gene polymorphism rs1981458
            with COVID-19 severity among Indian populations
  JOURNAL   Sci Rep 14 (1), 7822 (2024)
   PUBMED   38570613
  REMARK    GeneRIF: Novel genetic association of the Furin gene polymorphism
            rs1981458 with COVID-19 severity among Indian populations.
            Erratum:[Sci Rep. 2024 Apr 25;14(1):9523. doi:
            10.1038/s41598-024-60182-8.. PMID: 38664564]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4182)
  AUTHORS   Grinstein-Koren,O., Lusthaus,M., Tabibian-Keissar,H., Kaplan,I.,
            Buchner,A., Ilatov,R., Vered,M. and Zlotogorski-Hurvitz,A.
  TITLE     Pathological changes in oral epithelium and the expression of
            SARS-CoV-2 entry receptors, ACE2 and furin
  JOURNAL   PLoS One 19 (3), e0300269 (2024)
   PUBMED   38489333
  REMARK    GeneRIF: Pathological changes in oral epithelium and the expression
            of SARS-CoV-2 entry receptors, ACE2 and furin.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4182)
  AUTHORS   Shang,J., Wan,Y., Luo,C., Ye,G., Geng,Q., Auerbach,A. and Li,F.
  TITLE     Cell entry mechanisms of SARS-CoV-2
  JOURNAL   Proc Natl Acad Sci U S A 117 (21), 11727-11734 (2020)
   PUBMED   32376634
REFERENCE   6  (bases 1 to 4182)
  AUTHORS   Hoffmann,M., Kleine-Weber,H. and Pohlmann,S.
  TITLE     A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is
            Essential for Infection of Human Lung Cells
  JOURNAL   Mol Cell 78 (4), 779-784 (2020)
   PUBMED   32362314
REFERENCE   7  (bases 1 to 4182)
  AUTHORS   Klimpel,K.R., Molloy,S.S., Thomas,G. and Leppla,S.H.
  TITLE     Anthrax toxin protective antigen is activated by a cell surface
            protease with the sequence specificity and catalytic properties of
            furin
  JOURNAL   Proc Natl Acad Sci U S A 89 (21), 10277-10281 (1992)
   PUBMED   1438214
REFERENCE   8  (bases 1 to 4182)
  AUTHORS   Molloy,S.S., Bresnahan,P.A., Leppla,S.H., Klimpel,K.R. and
            Thomas,G.
  TITLE     Human furin is a calcium-dependent serine endoprotease that
            recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax
            toxin protective antigen
  JOURNAL   J Biol Chem 267 (23), 16396-16402 (1992)
   PUBMED   1644824
REFERENCE   9  (bases 1 to 4182)
  AUTHORS   Leduc,R., Molloy,S.S., Thorne,B.A. and Thomas,G.
  TITLE     Activation of human furin precursor processing endoprotease occurs
            by an intramolecular autoproteolytic cleavage
  JOURNAL   J Biol Chem 267 (20), 14304-14308 (1992)
   PUBMED   1629222
REFERENCE   10 (bases 1 to 4182)
  AUTHORS   Rehemtulla,A. and Kaufman,R.J.
  TITLE     Preferred sequence requirements for cleavage of pro-von Willebrand
            factor by propeptide-processing enzymes
  JOURNAL   Blood 79 (9), 2349-2355 (1992)
   PUBMED   1571548
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124248.6.
            
            Summary: This gene encodes a member of the subtilisin-like
            proprotein convertase family, which includes proteases that process
            protein and peptide precursors trafficking through regulated or
            constitutive branches of the secretory pathway. It encodes a type 1
            membrane bound protease that is expressed in many tissues,
            including neuroendocrine, liver, gut, and brain. The encoded
            protein undergoes an initial autocatalytic processing event in the
            ER and then sorts to the trans-Golgi network through endosomes
            where a second autocatalytic event takes place and the catalytic
            activity is acquired. Like other members of this convertase family,
            the product of this gene specifically cleaves substrates at single
            or paired basic residues. Some of its substrates include
            proparathyroid hormone, transforming growth factor beta 1
            precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix
            metalloproteinase, beta subunit of pro-nerve growth factor and von
            Willebrand factor. It is thought to be one of the proteases
            responsible for the activation of HIV envelope glycoproteins gp160
            and gp140, and may play a role in tumor progression. Unlike
            SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2
            is thought to be uniquely cleaved by this protease. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Aug 2020].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.1602600.1,
                                           SRR18074967.2366088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related :: involved in SARS-CoV-2 infection
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AC124248.6         114648-114706
            60-395              AC124248.6         118062-118397
            396-494             AC124248.6         118735-118833
            495-590             AC124248.6         118942-119037
            591-719             AC124248.6         119376-119504
            720-796             AC124248.6         119615-119691
            797-885             AC124248.6         120007-120095
            886-1058            AC124248.6         120612-120784
            1059-1271           AC124248.6         121244-121456
            1272-1372           AC124248.6         121924-122024
            1373-1476           AC124248.6         122151-122254
            1477-1594           AC124248.6         122347-122464
            1595-1774           AC124248.6         122574-122753
            1775-1899           AC124248.6         123171-123295
            1900-2010           AC124248.6         123410-123520
            2011-4182           AC124248.6         123766-125937
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..4182
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /note="furin, paired basic amino acid cleaving enzyme"
                     /db_xref="GeneID:5045"
                     /db_xref="HGNC:HGNC:8568"
                     /db_xref="MIM:136950"
     exon            1..59
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            60..395
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..2603
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /EC_number="3.4.21.75"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 5; proprotein convertase subtilisin/kexin type 3;
                     dibasic processing enzyme; furin, membrane associated
                     receptor protein; FES upstream region; paired basic amino
                     acid residue-cleaving enzyme; proprotein convertase
                     subtilisin/kexin 3; subtilisin-like proprotein convertase
                     1"
                     /codon_start=1
                     /product="furin isoform 1 preproprotein"
                     /protein_id="NP_001369549.1"
                     /db_xref="CCDS:CCDS10364.1"
                     /db_xref="GeneID:5045"
                     /db_xref="HGNC:HGNC:8568"
                     /db_xref="MIM:136950"
                     /translation="MELRPWLLWVVAATGTLVLLAADAQGQKVFTNTWAVRIPGGPAV
                     ANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAK
                     RRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNH
                     PDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYN
                     ARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFR
                     GVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACS
                     STLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRD
                     MQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKC
                     IIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSP
                     MGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKF
                     TLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLD
                     THYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSS
                     RESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRS
                     GFSFRGVKVYTMDRGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL"
     exon            396..494
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            495..590
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            591..719
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            720..796
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            797..885
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1058
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1271
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1372
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1476
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1594
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1774
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1899
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2010
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..4182
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4156..4161
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /note="hexamer: ATTAAA"
     polyA_site      4182
                     /gene="FURIN"
                     /gene_synonym="FUR; PACE; PCSK3; SPC1"
                     /note="major polyA site"
ORIGIN      
        1 gcacacctgt gcggccagga ctcgggtgcg ggatccgcgg gcgccggcgg ccggcgcagg
       61 gtcggcactc ttcaccctcc cgagccctgc ccgtctcggc cccatgcccc caccagtcag
      121 ccccgggcca caggcagtga gcaggcacct gggagccgag gccctgtgac caggccaagg
      181 agacgggcgc tccagggtcc cagccacctg tcccccccat ggagctgagg ccctggttgc
      241 tatgggtggt agcagcaaca ggaaccttgg tcctgctagc agctgatgct cagggccaga
      301 aggtcttcac caacacgtgg gctgtgcgca tccctggagg cccagcggtg gccaacagtg
      361 tggcacggaa gcatgggttc ctcaacctgg gccagatctt cggggactat taccacttct
      421 ggcatcgagg agtgacgaag cggtccctgt cgcctcaccg cccgcggcac agccggctgc
      481 agagggagcc tcaagtacag tggctggaac agcaggtggc aaagcgacgg actaaacggg
      541 acgtgtacca ggagcccaca gaccccaagt ttcctcagca gtggtacctg tctggtgtca
      601 ctcagcggga cctgaatgtg aaggcggcct gggcgcaggg ctacacaggg cacggcattg
      661 tggtctccat tctggacgat ggcatcgaga agaaccaccc ggacttggca ggcaattatg
      721 atcctggggc cagttttgat gtcaatgacc aggaccctga cccccagcct cggtacacac
      781 agatgaatga caacaggcac ggcacacggt gtgcggggga agtggctgcg gtggccaaca
      841 acggtgtctg tggtgtaggt gtggcctaca acgcccgcat tggaggggtg cgcatgctgg
      901 atggcgaggt gacagatgca gtggaggcac gctcgctggg cctgaacccc aaccacatcc
      961 acatctacag tgccagctgg ggccccgagg atgacggcaa gacagtggat gggccagccc
     1021 gcctcgccga ggaggccttc ttccgtgggg ttagccaggg ccgagggggg ctgggctcca
     1081 tctttgtctg ggcctcgggg aacgggggcc gggaacatga cagctgcaac tgcgacggct
     1141 acaccaacag tatctacacg ctgtccatca gcagcgccac gcagtttggc aacgtgccgt
     1201 ggtacagcga ggcctgctcg tccacactgg ccacgaccta cagcagtggc aaccagaatg
     1261 agaagcagat cgtgacgact gacttgcggc agaagtgcac ggagtctcac acgggcacct
     1321 cagcctctgc ccccttagca gccggcatca ttgctctcac cctggaggcc aataagaacc
     1381 tcacatggcg ggacatgcaa cacctggtgg tacagacctc gaagccagcc cacctcaatg
     1441 ccaacgactg ggccaccaat ggtgtgggcc ggaaagtgag ccactcatat ggctacgggc
     1501 ttttggacgc aggcgccatg gtggccctgg cccagaattg gaccacagtg gccccccagc
     1561 ggaagtgcat catcgacatc ctcaccgagc ccaaagacat cgggaaacgg ctcgaggtgc
     1621 ggaagaccgt gaccgcgtgc ctgggcgagc ccaaccacat cactcggctg gagcacgctc
     1681 aggcgcggct caccctgtcc tataatcgcc gtggcgacct ggccatccac ctggtcagcc
     1741 ccatgggcac ccgctccacc ctgctggcag ccaggccaca tgactactcc gcagatgggt
     1801 ttaatgactg ggccttcatg acaactcatt cctgggatga ggatccctct ggcgagtggg
     1861 tcctagagat tgaaaacacc agcgaagcca acaactatgg gacgctgacc aagttcaccc
     1921 tcgtactcta tggcaccgcc cctgaggggc tgcccgtacc tccagaaagc agtggctgca
     1981 agaccctcac gtccagtcag gcctgtgtgg tgtgcgagga aggcttctcc ctgcaccaga
     2041 agagctgtgt ccagcactgc cctccagggt tcgcccccca agtcctcgat acgcactata
     2101 gcaccgagaa tgacgtggag accatccggg ccagcgtctg cgccccctgc cacgcctcat
     2161 gtgccacatg ccaggggccg gccctgacag actgcctcag ctgccccagc cacgcctcct
     2221 tggaccctgt ggagcagact tgctcccggc aaagccagag cagccgagag tccccgccac
     2281 agcagcagcc acctcggctg cccccggagg tggaggcggg gcaacggctg cgggcagggc
     2341 tgctgccctc acacctgcct gaggtggtgg ccggcctcag ctgcgccttc atcgtgctgg
     2401 tcttcgtcac tgtcttcctg gtcctgcagc tgcgctctgg ctttagtttt cggggggtga
     2461 aggtgtacac catggaccgt ggcctcatct cctacaaggg gctgccccct gaagcctggc
     2521 aggaggagtg cccgtctgac tcagaagagg acgagggccg gggcgagagg accgccttta
     2581 tcaaagacca gagcgccctc tgatgagccc actgcccacc ccctcaagcc aatcccctcc
     2641 ttgggcactt tttaattcac caaagtattt ttttatcttg ggactgggtt tggaccccag
     2701 ctgggaggca agaggggtgg agactgcttc ccatcctacc ctcgggccca cctggccacc
     2761 tgaggtgggc ccaggaccag ctggggcgtg gggagggccg taccccaccc tcagcacccc
     2821 ttccatgtgg agaaaggagt gaaaccttta gggcagcttg ccccggcccc ggccccagcc
     2881 agagttcctg cggagtgaag aggggcagcc cttgcttgtt gggattcctg acccaggccg
     2941 cagctcttgc ccttccctgt ccctctaaag caataatggt cccatccagg cagtcggggg
     3001 ctggcctagg agatatctga gggaggaggc cacctctcca agggcttctg caccctccac
     3061 cctgtccccc agctctggtg agtcttggcg gcagcagcca tcataggaag ggaccaaggc
     3121 aaggcaggtg cctccaggtg tgcacgtggc atgtggcctg tggcctgtgt cccatgaccc
     3181 acccctgtgc tccgtgcctc caccaccact ggccaccagg ctggcgcagc caaggccgaa
     3241 gctctggctg aaccctgtgc tggtgtcctg accaccctcc cctctcttgc acccgcctct
     3301 cccgtcaggg cccaagtccc tgttttctga gcccgggctg cctgggctgt tggcactcac
     3361 agacctggag cccctgggtg ggtggtgggg aggggcgctg gcccagccgg cctctctggc
     3421 ctcccacccg atgctgcttt cccctgtggg gatctcaggg gctgtttgag gatatatttt
     3481 cactttgtga ttatttcact ttagatgctg atgatttgtt tttgtatttt taatgggggt
     3541 agcagctgga ctacccacgt tctcacaccc accgtccgcc ctgctcctcc ctggctgccc
     3601 tggccctgag gtgtgggggc tgcagcatgt tgctgaggag tgaggaatag ttgagcccca
     3661 agtcctgaag aggcgggcca gccaggcggg ctcaaggaaa gggggtccca gtgggagggg
     3721 caggctgaca tctgtgtttc aagtggggct cgccatgccg ggggttcata ggtcactggc
     3781 tctccaagtg ccagaggtgg gcaggtggtg gcactgagcc cccccaacac tgtgccctgg
     3841 tggagaaagc actgacctgt catgcccccc tcaaacctcc tcttctgacg tgccttttgc
     3901 acccctccca ttaggacaat cagtcccctc ccatctggga gtcccctttt cttttctacc
     3961 ctagccattc ctggtaccca gccatctgcc caggggtgcc ccctcctctc ccatccccct
     4021 gccctcgtgg ccagcccggc tggttttgta agatgctggg ttggtgcaca gtgatttttt
     4081 tcttgtaatt taaacaggcc cagcattgct ggttctattt aatggacatg agataatgtt
     4141 agaggtttta aagtgattaa acgtgcagac tatgcaaacc ag
//
