LOCUS       NM_001102371            4906 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens FAD dependent oxidoreductase domain containing 2
            (FOXRED2), transcript variant 2, mRNA.
ACCESSION   NM_001102371
VERSION     NM_001102371.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   3  (bases 1 to 4906)
  AUTHORS   Hunter,F.W., Jaiswal,J.K., Hurley,D.G., Liyanage,H.D.,
            McManaway,S.P., Gu,Y., Richter,S., Wang,J., Tercel,M., Print,C.G.,
            Wilson,W.R. and Pruijn,F.B.
  TITLE     The flavoprotein FOXRED2 reductively activates
            nitro-chloromethylbenzindolines and other hypoxia-targeting
            prodrugs
  JOURNAL   Biochem Pharmacol 89 (2), 224-235 (2014)
   PUBMED   24632291
  REMARK    GeneRIF: Expression of FOXRED2 increases activity of
            hypoxia-targeted prodrugs.
REFERENCE   4  (bases 1 to 4906)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4906)
  AUTHORS   Shim,S., Lee,W., Chung,H. and Jung,Y.K.
  TITLE     Amyloid beta-induced FOXRED2 mediates neuronal cell death via
            inhibition of proteasome activity
  JOURNAL   Cell Mol Life Sci 68 (12), 2115-2127 (2011)
   PUBMED   20972601
REFERENCE   6  (bases 1 to 4906)
  AUTHORS   Riemer,J., Hansen,H.G., Appenzeller-Herzog,C., Johansson,L. and
            Ellgaard,L.
  TITLE     Identification of the PDI-family member ERp90 as an interaction
            partner of ERFAD
  JOURNAL   PLoS One 6 (2), e17037 (2011)
   PUBMED   21359175
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4906)
  AUTHORS   Riemer,J., Appenzeller-Herzog,C., Johansson,L., Bodenmiller,B.,
            Hartmann-Petersen,R. and Ellgaard,L.
  TITLE     A luminal flavoprotein in endoplasmic reticulum-associated
            degradation
  JOURNAL   Proc Natl Acad Sci U S A 106 (35), 14831-14836 (2009)
   PUBMED   19706418
  REMARK    GeneRIF: ERFAD facilitates the dislocation of certain endoplasmic
            reticulum-associated degradation substrates to the cytosol.
REFERENCE   8  (bases 1 to 4906)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   9  (bases 1 to 4906)
  AUTHORS   Imabayashi,H., Mori,T., Gojo,S., Kiyono,T., Sugiyama,T., Irie,R.,
            Isogai,T., Hata,J., Toyama,Y. and Umezawa,A.
  TITLE     Redifferentiation of dedifferentiated chondrocytes and
            chondrogenesis of human bone marrow stromal cells via chondrosphere
            formation with expression profiling by large-scale cDNA analysis
  JOURNAL   Exp Cell Res 288 (1), 35-50 (2003)
   PUBMED   12878157
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC040351.1, AL022313.1 and BG054783.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001102371.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1 and encodes isoform 1. Variants 1 and 2
            encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK025714.1, SRR1803613.266297.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397224.9/ ENSP00000380401.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3174              BC040351.1         24-3197
            3175-4623           AL022313.1         39771-41219         c
            4624-4906           BG054783.1         14-296              c
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.3"
     gene            1..4906
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /note="FAD dependent oxidoreductase domain containing 2"
                     /db_xref="GeneID:80020"
                     /db_xref="HGNC:HGNC:26264"
                     /db_xref="MIM:613777"
     exon            1..32
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            33..560
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..2088
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 2; endoplasmic reticulum flavoprotein associated
                     with degradation; FAD-dependent oxidoreductase
                     domain-containing protein 2; ER flavoprotein associated
                     with degradation"
                     /codon_start=1
                     /product="FAD-dependent oxidoreductase domain-containing
                     protein 2 isoform 1 precursor"
                     /protein_id="NP_001095841.1"
                     /db_xref="CCDS:CCDS13929.1"
                     /db_xref="GeneID:80020"
                     /db_xref="HGNC:HGNC:26264"
                     /db_xref="MIM:613777"
                     /translation="MGLSAAAPLWGPPGLLLAIALHPALSVPPRRDYCVLGAGPAGLQ
                     MAYFLQRAGRDYAVFERAPRPGSFFTRYPRHRKLISINKRYTGKANAEFNLRHDWNSL
                     LSHDPRLLFRHYSRAYFPDARDMVRYLGDFADTLGLRVQYNTTIAHVTLDKDRQAWNG
                     HYFILTDQKGQVHQCSVLFVATGLSVPNQVDFPGSEYAEGYESVSVDPEDFVGQNVLI
                     LGRGNSAFETAENILGVTNFIHMLSRSRVRLSWATHYVGDLRAINNGLLDTYQLKSLD
                     GLLESDLTDLAILKDSKGKFHVTPKFFLEEANTNQSADSITLPQDDNDNFAMRVPYDR
                     VIRCLGWNFDFSIFNKSLRLNSGNAFGKKYPLIRASYESKGSRGLFILGTASHSVDYR
                     KSAGGFIHGFRYTVRAVHRLLEHRHHSVTWPATELPITQLTSSIVRRVNEASGLYQMF
                     GVLADVILLKENSTAFEYLEEFPIQMLAQLETLTGRKAKHGLFVINMEYGRNFSGPDK
                     DVFFDDRSVGHTEDAWQSNFLHPVIYYYRYLPTEQEVRFRPAHWPLPRPTAIHHIVED
                     FLTDWTAPIGHILPLRRFLENCLDTDLRSFYAESCFLFALTRQKLPPFCQQGYLRMQG
                     LVSTESLWQHRVESRLLRDYAPTGRRLEDSSQQLGDQEPLGSPLAPGPLAQSVDSNKE
                     EL"
     exon            561..812
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            813..1082
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1249
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1415
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1657
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1828
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..4906
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4883..4888
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /note="hexamer: AGTAAA"
     polyA_site      4906
                     /gene="FOXRED2"
                     /gene_synonym="ERFAD"
                     /note="major polyA site"
ORIGIN      
        1 agaccacggg ttgcagccgg agcggctggg aggatgggcc tctccgctgc ggccccgttg
       61 tggggtcccc cggggctgct cctggccatc gccctgcacc cagcgctgtc ggtgcccccg
      121 cgccgggact actgcgtgct gggcgctggg cccgcgggcc tgcagatggc ctacttcctg
      181 cagcgcgctg gacgcgacta cgcagtgttc gagcgggccc cgcggcccgg cagcttcttc
      241 acacgctacc cgcggcaccg caagctcatc agcatcaaca agcggtacac gggcaaggct
      301 aacgccgagt tcaacctccg ccacgactgg aactctctgc tcagccacga cccccggctg
      361 ctcttcagac actactcgcg tgcctacttc cccgacgccc gcgacatggt gcgctacctg
      421 ggtgacttcg cggacacgct ggggctccgt gtccagtaca acaccaccat cgcccacgtc
      481 actctggaca aggaccgaca ggcctggaat ggccactact tcatcctaac tgaccagaag
      541 ggccaggtgc atcagtgcag cgtcctcttt gtagccactg gtttatcagt ccccaaccag
      601 gttgacttcc ctggctccga atatgcagag ggttacgagt ccgtgtccgt ggaccctgag
      661 gactttgtag gccagaatgt gctgatcctg ggtcgtggga actcggcctt tgagacagca
      721 gagaacatct tgggtgtcac aaactttatc catatgctca gccgctcccg ggtccgtctg
      781 tcctgggcca cccactacgt tggagacctc agagccatca acaatggcct gctggatacc
      841 taccagctca agtccctgga cgggctgctc gagtctgacc tgacggatct ggccatcctg
      901 aaggacagca aaggcaagtt ccatgtcacc ccgaaattct tcctggaaga agccaacacc
      961 aaccagagtg ccgactccat caccctcccc caggacgaca atgacaactt tgccatgcgc
     1021 gtgccctatg accgggtaat ccgctgcctg ggctggaact ttgacttctc cattttcaat
     1081 aagtccctca gacttaactc gggaaatgca ttcggcaaga agtacccgct gattcgagct
     1141 agctacgaat ccaaaggaag ccggggtctg tttatcctgg gtactgccag ccactcggtg
     1201 gactaccgga aatctgctgg gggcttcatc cacggattcc gatacacagt gcgtgctgtt
     1261 caccggctcc tggagcaccg ccaccacagc gtcacctggc ccgccactga gctccccatc
     1321 acacagctga ccagctccat cgtgcggcgc gtgaatgagg cttctgggct ctaccagatg
     1381 ttcggtgtgc tggccgatgt catcctgttg aaggagaatt ccacggcctt tgagtacctg
     1441 gaggagttcc ccatacagat gctggcccag ctggagacac tcacagggag gaaggcaaag
     1501 cacgggctct tcgtcatcaa catggaatat ggcagaaatt tctctggccc cgacaaggac
     1561 gtcttctttg atgaccggtc tgtggggcac acagaagatg cctggcagtc taactttctt
     1621 catcctgtca tctactacta tagatacctc cccaccgaac aggaggtgag gttccgccct
     1681 gcacactggc ccctgcctcg gcccacggcc atccatcaca tcgtggaaga cttcttaaca
     1741 gactggactg ccccgatcgg gcacatccta cctctgaggc gcttcctgga gaactgtttg
     1801 gacaccgatt tgcgaagctt ctatgcagag tcctgcttcc tgttcgccct cacgcgccag
     1861 aagttgccac ccttttgcca gcaggggtac ctgaggatgc agggactcgt gagtaccgag
     1921 agcctttggc agcacagagt ggagagcagg ctcctgcggg actatgcccc cacaggcagg
     1981 cgcctggagg acagcagcca gcagcttggc gaccaagagc cactaggttc ccccctggct
     2041 ccagggcctc tggctcagtc cgtcgatagc aacaaagagg agctctgact gtcccttcct
     2101 aagctgtggg cacagtggcc aggcctcccc acctaggccc atggtcagtc cctctttccc
     2161 cgcaacctca cactcccgcc aatgcgtgat tgccaaagac cactcagatc atggcagtgt
     2221 ctgcagcaaa gccaccagat ggcacgtgag ggctgaaagt cggggatgcg gtggtctcct
     2281 ccctcctgtc agggggcaga agggcatcct ctgccaggtg ggagctgcct tccccagccc
     2341 agtttgtctg caatggagct gtcgctgagc cccaggccag cccagttctg ccccgtctca
     2401 agtgctcctc ttccttccaa gcccctcctc agatgggcct gcatcccgcc tgtggagtgc
     2461 ttcacagggg aaacccatcc gcatcttaag agtttgtggc aagcgcttta aaacaaaagc
     2521 tctgatatcc tcaacaacca caatgggcag gtgggattgt tgccccagat gactgctgaa
     2581 gacaccagct gtgggatgtc aagtaattcc agggaccccc agctgggagt ccccgtctgt
     2641 gtcacttgcc acacactgcc tcaacctccc cgccccattt tcagcagggt ttgggcttag
     2701 ctacagagcc caagagggtc agccctgctt atcatcatag gcccagcagg ctgatctcga
     2761 gcagcgatag gacagctccc tgcaaagcca tcttagcaac tttctcactg agaagcagga
     2821 ttcagagctg ctctccccag ttgcaccccc tgactcatct tccagctggc tcttctctcc
     2881 catcctactc ccttctgtct ccctgccctc tgccatcctg aaccatgcct tgcttctaac
     2941 ctgcctggtc aagttttgtg aaacttatcc tgctgtctcg ggctggcacc catatgtgct
     3001 caccatgtgt aaggtgacat ttccaaatgc ctttgtgtga taatgatgat gaatttggtt
     3061 cctagtctct tcttcattgc ccttcctcac cagcaatctg cactttccca tcaggcgtct
     3121 tagtctcatt tgtaagtgca taagataatc acagtgattt ttcaaagccc aggatcataa
     3181 gtgattgctt tttcactggt tcttactaac tcttcatgta tccactcaga attccaagga
     3241 caatcccagc actctgggag gcccaggcag gtggatcacc tgaggtcagg agtttgagac
     3301 cagcctgacc aacatggaga aaccctatct ctactaaaaa tacaaaacta gccaggcgtg
     3361 gtagcacatg tctataatcc cagctactgg agaggctgag gcaggagaat cacttaaacc
     3421 cgggaggcag aggttgcagt gagccgagat tgcgccattg cactccagcc taggtaacaa
     3481 gagggaaact ccatctcaaa aaataagtta attaaattaa attaaaataa aacatgcgct
     3541 gggtacagtt gcttacacct gtaatcccag cactttggga ggccaaagtg ggccgatcac
     3601 ctgagctcag gaattcgaga ccagcctggc caacatagca aaaccttgtc tctactaacg
     3661 gtaaaaaaaa ttagctgggc atggtggagc atgcctgtca tcccagctac ttgggaagct
     3721 gaggcaggag aattgcttga acctgggagg tggaggttgc agtgagcaga gaccgagcca
     3781 ctgtactcca gcctaggtga caaagcaaga cccacagtaa agcccccaca ccccatctca
     3841 aaaaaataaa aaataaaaaa gaacaaagta tgctacaaca aaagtgaaaa gagaaaaaga
     3901 attccaagaa cccgggcttt ctggatccaa agctctttca tggggacgtc ctcttcccct
     3961 cttggcttgg tccagacttt ttctttttct tttttttttt ttttgcaatg gatttcgctc
     4021 ttgtcaccca ggctggagtg caatggcacg atgtcggctc actgcaactt ctgcctcccg
     4081 ggtctaaaca attctcttcc ctcaacctcc caagtagctg ggattacaag cgcctgccac
     4141 cacgcccagc taatttttgt atttttagta gagacagggt ttcaccacat tggccaggct
     4201 ggtctcgaac tcctgacctc aggtgatcca cctgtcttgg cctcctaaag tgttgggatt
     4261 acaggcatga gccaccacgc tcggcccagt ccagactttt taactacctg gccccactgc
     4321 agacacagtt ccctctagtc cttcccagag ttacaagaag agaactccag tagccttatt
     4381 ctgtcaggga cgaattgttt accccatgat aatgtaggat atcataaatt cttcttcaaa
     4441 ggttttagcc tgtaatttgt taagtacatt gagttctgag atcctctcca aagaaccaat
     4501 gtatcagtat gttcagctca cctgttcttt gttcttcatt ttaactttta atttcctcat
     4561 tctttatgtc tccttgcccc tagtttcagt aaacaatccc ctcctagcct ctgtcacctg
     4621 ctctgacctg agtcaccttt agtcacctgg tccgtaacca tctttcctgt ctaaacttct
     4681 caccccacca ctctggctta tacccctgct ctctttaaaa tagccagtca gaattagctt
     4741 agattgtgcg gtccaaccct agcccatagg ggaacaacac agcagtaggg ggtacctgca
     4801 tcagggataa gaacccattc ccctcccttg ttccggtgtg ctctcgccat tgcaccatcc
     4861 atgagacgca ctcttgtata gaagtaaaat tgccttgctg agaaaa
//
