LOCUS       NM_153756               2831 bp    mRNA    linear   PRI 31-JAN-2025
DEFINITION  Homo sapiens fibronectin type III domain containing 5 (FNDC5),
            transcript variant 2, mRNA.
ACCESSION   NM_153756
VERSION     NM_153756.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Chen,Q., Wang,J., Li,K., Luan,J.Q., Li,J.M. and Wang,Y.T.
  TITLE     Irisin in thyroid diseases
  JOURNAL   Clin Chim Acta 564, 119929 (2025)
   PUBMED   39154700
  REMARK    GeneRIF: Irisin in thyroid diseases.
            Review article
            Erratum:[Clin Chim Acta. 2025 Jan 30;566:120034. doi:
            10.1016/j.cca.2024.120034. PMID: 39550251]
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Zhao,F., Xu,D., Wang,X. and Wang,X.
  TITLE     FNDC5 affects invasion and migration of oral cancer by inhibiting
            PI3K/Akt/Snail signaling pathway
  JOURNAL   Sci Rep 14 (1), 26881 (2024)
   PUBMED   39505986
  REMARK    GeneRIF: FNDC5 affects invasion and migration of oral cancer by
            inhibiting PI3K/Akt/Snail signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2831)
  AUTHORS   Gliwinska,A., Badenska,M., Dworak,M., Swietochowska,E.,
            Badenski,A., Bjanid,O., Trembecka-Dubel,E., Morawiec-Knysak,A. and
            Szczepanska,M.
  TITLE     Assessment of brain-derived neurotrophic factor and irisin
            concentration in children with chronic kidney disease: a pilot
            study
  JOURNAL   BMC Nephrol 25 (1), 318 (2024)
   PUBMED   39334009
  REMARK    GeneRIF: Assessment of brain-derived neurotrophic factor and irisin
            concentration in children with chronic kidney disease: a pilot
            study.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2831)
  AUTHORS   Radikova,Z., Mosna,L., Eckerstorfer,C., Bajer,B., Havranova,A.,
            Imrich,R., Vlcek,M. and Penesova,A.
  TITLE     Plasma irisin and the brain-derived neurotrophic factor levels in
            sedentary subjects: effect of 8-weeks lifestyle intervention
  JOURNAL   Endocr Regul 58 (1), 115-128 (2024)
   PUBMED   38861537
  REMARK    GeneRIF: Plasma irisin and the brain-derived neurotrophic factor
            levels in sedentary subjects: effect of 8-weeks lifestyle
            intervention.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2831)
  AUTHORS   Lecker,S.H., Zavin,A., Cao,P., Arena,R., Allsup,K., Daniels,K.M.,
            Joseph,J., Schulze,P.C. and Forman,D.E.
  TITLE     Expression of the irisin precursor FNDC5 in skeletal muscle
            correlates with aerobic exercise performance in patients with heart
            failure
  JOURNAL   Circ Heart Fail 5 (6), 812-818 (2012)
   PUBMED   23001918
REFERENCE   6  (bases 1 to 2831)
  AUTHORS   Wu,J., Bostrom,P., Sparks,L.M., Ye,L., Choi,J.H., Giang,A.H.,
            Khandekar,M., Virtanen,K.A., Nuutila,P., Schaart,G., Huang,K.,
            Tu,H., van Marken Lichtenbelt,W.D., Hoeks,J., Enerback,S.,
            Schrauwen,P. and Spiegelman,B.M.
  TITLE     Beige adipocytes are a distinct type of thermogenic fat cell in
            mouse and human
  JOURNAL   Cell 150 (2), 366-376 (2012)
   PUBMED   22796012
REFERENCE   7  (bases 1 to 2831)
  AUTHORS   Kelly,D.P.
  TITLE     Medicine. Irisin, light my fire
  JOURNAL   Science 336 (6077), 42-43 (2012)
   PUBMED   22491843
REFERENCE   8  (bases 1 to 2831)
  AUTHORS   Bostrom,P., Wu,J., Jedrychowski,M.P., Korde,A., Ye,L., Lo,J.C.,
            Rasbach,K.A., Bostrom,E.A., Choi,J.H., Long,J.Z., Kajimura,S.,
            Zingaretti,M.C., Vind,B.F., Tu,H., Cinti,S., Hojlund,K., Gygi,S.P.
            and Spiegelman,B.M.
  TITLE     A PGC1-alpha-dependent myokine that drives brown-fat-like
            development of white fat and thermogenesis
  JOURNAL   Nature 481 (7382), 463-468 (2012)
   PUBMED   22237023
  REMARK    GeneRIF: Experimental evidence (Western and mass spectrometry) of
            mouse and human secreted irisin peptide
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2831)
  AUTHORS   Ivanov,I.P., Firth,A.E., Michel,A.M., Atkins,J.F. and Baranov,P.V.
  TITLE     Identification of evolutionarily conserved non-AUG-initiated
            N-terminal extensions in human coding sequences
  JOURNAL   Nucleic Acids Res 39 (10), 4220-4234 (2011)
   PUBMED   21266472
  REMARK    GeneRIF: infers non-AUG translation initiation codon for FNDC5
            based on evolutionary comparisons; only human has an ''ATA'' at a
            position that is ''ATG'' in other mammals
REFERENCE   10 (bases 1 to 2831)
  AUTHORS   Teufel,A., Malik,N., Mukhopadhyay,M. and Westphal,H.
  TITLE     Frcp1 and Frcp2, two novel fibronectin type III repeat containing
            genes
  JOURNAL   Gene 297 (1-2), 79-83 (2002)
   PUBMED   12384288
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114493.3, BE502835.1,
            AK092102.1 and BC033123.1.
            
            On Nov 30, 2018 this sequence version replaced NM_153756.2.
            
            Summary: This gene encodes a secreted protein that is released from
            muscle cells during exercise. The encoded protein may participate
            in the development of brown fat. Translation of the precursor
            protein initiates at a non-AUG start codon at a position that is
            conserved as an AUG start codon in other organisms. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jun 2013].
            
            Transcript Variant: This variant (2) contains multiple differences
            in the UTRs and coding region, compared to variant 1. It initiates
            translation from an alternate 'AUA' start codon that is conserved
            as an 'AUG' start codon in orthologs. The encoded isoform (2) is
            longer and has distinct N- and C-termini, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK092102.1, ERR4352443.293130.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968189,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000373471.9/
                                                ENSP00000362570.5
            non-AUG initiation codon         :: inferred from conservation
            RefSeq Select criteria           :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AC114493.3         105834-105932       c
            100-166             BE502835.1         2-68
            167-2534            AK092102.1         1-2368
            2535-2831           BC033123.1         1268-1564
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.1"
     gene            1..2831
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /note="fibronectin type III domain containing 5"
                     /db_xref="GeneID:252995"
                     /db_xref="HGNC:HGNC:20240"
                     /db_xref="MIM:611906"
     exon            1..260
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..805
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /exception="alternative start codon"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; non-AUG (AUA) translation initiation codon;
                     fibronectin type III repeat-containing protein 2"
                     /codon_start=1
                     /transl_except=(pos:167..169,aa:Met)
                     /product="fibronectin type III domain-containing protein 5
                     isoform 2 preproprotein"
                     /protein_id="NP_715637.2"
                     /db_xref="CCDS:CCDS369.2"
                     /db_xref="GeneID:252995"
                     /db_xref="HGNC:HGNC:20240"
                     /db_xref="MIM:611906"
                     /translation="MHPGSPSAWPPRARAALRLWLGCVCFALVQADSPSAPVNVTVRH
                     LKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYI
                     VHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKEMGRNQQLRTGEVLIIVV
                     VLFMWAGVIALFCRQYDIIKDNEPNNNKEKTKSASETSTPEHQGGGLLRSKI"
     exon            261..376
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /inference="alignment:Splign:2.1.0"
     exon            377..575
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /inference="alignment:Splign:2.1.0"
     exon            576..665
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /inference="alignment:Splign:2.1.0"
     exon            666..799
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /inference="alignment:Splign:2.1.0"
     exon            800..2831
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2808..2813
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /note="hexamer: AATAAA"
     polyA_site      2831
                     /gene="FNDC5"
                     /gene_synonym="FRCP2; irisin"
                     /note="major polyA site"
ORIGIN      
        1 agtcgctgtc cgcggagccg acatgcaggc ggctcggggc ggcgcagggc ggccggagcg
       61 gccgggccgg ccgggccggg ggccggagcg cgagcgcgag cggccgccgg gcgccggagc
      121 cgcgtccccc tgcgccgccc cgggcctgcc ggccggagga gccaccatac accccgggtc
      181 gccgagcgcc tggccgcccc gcgcccgcgc cgcgctccgc ctgtggctgg gctgcgtctg
      241 cttcgcgctg gtgcaggcgg acagtccctc agccccagtg aacgtcaccg tcaggcacct
      301 caaggccaac tctgcagtgg tgagctggga tgttctggag gatgaggttg tcatcggatt
      361 tgccatctcc cagcagaaga aggatgtgcg gatgctgcgc ttcatccagg aggtgaacac
      421 caccacccgc tcatgtgccc tctgggacct ggaggaggat acggagtaca tagtccacgt
      481 gcaggccatc tccattcagg gccagagccc agccagcgag cctgtgctct tcaagacccc
      541 gcgtgaggct gagaagatgg cctccaagaa caaagatgag gtaaccatga aagagatggg
      601 gaggaaccaa cagctgcgga caggcgaggt gctgatcatc gtcgtggtcc tgttcatgtg
      661 ggcaggtgtc attgccctct tctgccgcca gtatgacatc atcaaggaca atgaacccaa
      721 taacaacaag gaaaaaacca agagtgcatc agaaaccagc acaccagagc accagggcgg
      781 ggggcttctc cgcagcaaga tatgaaaacc ttttcagtgc ttgccctcag cagctaagaa
      841 gacagacagt agagaatgtg agaggatctc atggttctga tgatgattat ccaacaaaca
      901 tctggccctc tctacatctc ttccctccat ctccttgtac cctctggctt actgtcctct
      961 ctctggcgca ctttcctgaa gcctcttatt aactcccatc tccagaagca cctcaacaat
     1021 gtcagtggct gaggctgcac tcagagggat gactgctggg ggtagactgg gtgccagggg
     1081 ccatgggccc aggacccagt cttggccatt cagttgagtg aggctgaagg ctgggtttga
     1141 aaaggcaaaa agacaagaca tccaggcagg gcttctcttt tctttccaca agggacaaga
     1201 gcttggctta tttaggctac agccctgctg ctgctccctt ccttcttctg tcctgtttcc
     1261 agccttgcaa gaaggctatt cacaattagg cctggctgct tctctcattt ttttcctccc
     1321 agtttccaaa caagccctca gtgaacatca ttgaagcgtg actgcctgtc tgcagggaga
     1381 aggattccat ttttcttctc agctggtccc caggcccacg ggcacaggga gagggacaac
     1441 tgcagcagtg gggaggaggc acagctagct gcacagttct ctcttctcct tgtcctagtc
     1501 agatgaagga ggctgcacta caaacccaaa ttctgcaaaa aaataaaaat aagccacaaa
     1561 actaaaaggc ctggccccat tctggaaaag gcaaagctgc atgagacaca gccttctgcc
     1621 tcctcgcctc tcctggactg gcttcctctt tgagaaaatg cacaaagccc tgggagatga
     1681 caagcacaag gactgactca agctgtgtct ttcagaccaa ggaacatcag agaagctgtg
     1741 gggctgcctg ccaggcagga tcatggctgc catcaagcct tttctggatc cagccatcaa
     1801 ggacatgttt gtggtgtgat gcacactttt gcaagcgtgt aagatgttac ctggtttgtc
     1861 tcttttggaa aacaaaaatc agaaggctgc attctagagg gcagagaaat tcccccgaag
     1921 actgagctgg ttgcctgcat cctctatctt ctttgaccct tatgactgaa agatcatcag
     1981 tttggaaggt actggtccaa tttatttagg aagtatctct tggagtttca gaaatgctag
     2041 cttggacaac tgaaaagtca catcacagct ggcattctgg gggctaccaa aacacccctt
     2101 ctggagtaga agctgctgga aggcaggcct gagccattca ccacggacag gaagagcagc
     2161 tctggctatc accactggcc tctggggtct tcatatcttg ccatctcatc cagggttcca
     2221 tgaaagttac ccagggtcct catgtccttc cttagagcct gagtggtgtg aggtgacagg
     2281 tctctctctc cactgcccct ttctggttta aaaaaatggt gcttgatgag ggaaggtaga
     2341 ctcttcccta ggactgacga gttacggctg ccagatgcct gcatgggaag aggtggacat
     2401 ctgcatcttc cattggtggt caaggatggg tgtgggagaa ccacacctag tgcaagcctg
     2461 gtactcagta aatatttgtt gaaatgaatg ataagagcat tggtccccaa gccagagagc
     2521 cagaagccat cacccaatga ccgccccttc cttccggtct acaagagctc tcaaggctgg
     2581 gtctgccacc actctgcttt gcccaagtgt gacagcactg gggaggagag acaggataaa
     2641 gggcagatgt cagcaatact aagggcttcc tcatgggagg gcatgaggct ccactcattg
     2701 tcttgtgact tccatccctg ctgaatgggg ctgcaaggcc aaggctcctt aggggagagg
     2761 tccttacctc tgatccactt agagcaataa ccacttttta aatgtaaaat aaaaagacaa
     2821 atgaaaaggc a
//
