LOCUS       NM_001461               2331 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens flavin containing dimethylaniline monoxygenase 5
            (FMO5), transcript variant 1, mRNA.
ACCESSION   NM_001461
VERSION     NM_001461.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Fiorentini,F., Romero,E., Fraaije,M.W., Faber,K., Hall,M. and
            Mattevi,A.
  TITLE     Baeyer-Villiger Monooxygenase FMO5 as Entry Point in Drug
            Metabolism
  JOURNAL   ACS Chem Biol 12 (9), 2379-2387 (2017)
   PUBMED   28783300
  REMARK    GeneRIF: Nabumetone and pentoxifylline, two widely used
            pharmaceuticals, were thereby demonstrated to be efficiently
            oxidized in vitro by FMO5 to the corresponding acetate esters with
            high selectivity.
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Chen,Y., Zane,N.R., Thakker,D.R. and Wang,M.Z.
  TITLE     Quantification of Flavin-containing Monooxygenases 1, 3, and 5 in
            Human Liver Microsomes by UPLC-MRM-Based Targeted Quantitative
            Proteomics and Its Application to the Study of Ontogeny
  JOURNAL   Drug Metab Dispos 44 (7), 975-983 (2016)
   PUBMED   26839369
  REMARK    GeneRIF: Report relatively stable FMO5 expression throughout
            development.
REFERENCE   3  (bases 1 to 2331)
  AUTHORS   Fiorentini,F., Geier,M., Binda,C., Winkler,M., Faber,K., Hall,M.
            and Mattevi,A.
  TITLE     Biocatalytic Characterization of Human FMO5: Unearthing
            Baeyer-Villiger Reactions in Humans
  JOURNAL   ACS Chem Biol 11 (4), 1039-1048 (2016)
   PUBMED   26771671
  REMARK    GeneRIF: absence of the typical characteristic sequence motifs sets
            human FMO5 apart from all characterized Baeyer-Villiger
            mono-oxygenases so far. These findings open new perspectives in
            human oxidative metabolism
REFERENCE   4  (bases 1 to 2331)
  AUTHORS   Lopez,S., Buil,A., Souto,J.C., Casademont,J., Martinez-Perez,A.,
            Almasy,L. and Soria,J.M.
  TITLE     A genome-wide association study in the genetic analysis of
            idiopathic thrombophilia project suggests sex-specific regulation
            of mitochondrial DNA levels
  JOURNAL   Mitochondrion 18, 34-40 (2014)
   PUBMED   25240745
REFERENCE   5  (bases 1 to 2331)
  AUTHORS   Lai,W.G., Farah,N., Moniz,G.A. and Wong,Y.N.
  TITLE     A Baeyer-Villiger oxidation specifically catalyzed by human
            flavin-containing monooxygenase 5
  JOURNAL   Drug Metab Dispos 39 (1), 61-70 (2011)
   PUBMED   20947616
REFERENCE   6  (bases 1 to 2331)
  AUTHORS   McCombie,R.R., Dolphin,C.T., Povey,S., Phillips,I.R. and
            Shephard,E.A.
  TITLE     Localization of human flavin-containing monooxygenase genes FMO2
            and FMO5 to chromosome 1q
  JOURNAL   Genomics 34 (3), 426-429 (1996)
   PUBMED   8786146
REFERENCE   7  (bases 1 to 2331)
  AUTHORS   Phillips,I.R., Dolphin,C.T., Clair,P., Hadley,M.R., Hutt,A.J.,
            McCombie,R.R., Smith,R.L. and Shephard,E.A.
  TITLE     The molecular biology of the flavin-containing monooxygenases of
            man
  JOURNAL   Chem Biol Interact 96 (1), 17-32 (1995)
   PUBMED   7720101
REFERENCE   8  (bases 1 to 2331)
  AUTHORS   Overby,L.H., Buckpitt,A.R., Lawton,M.P., Atta-Asafo-Adjei,E.,
            Schulze,J. and Philpot,R.M.
  TITLE     Characterization of flavin-containing monooxygenase 5 (FMO5) cloned
            from human and guinea pig: evidence that the unique catalytic
            properties of FMO5 are not confined to the rabbit ortholog
  JOURNAL   Arch Biochem Biophys 317 (1), 275-284 (1995)
   PUBMED   7872795
REFERENCE   9  (bases 1 to 2331)
  AUTHORS   Hines,R.N., Cashman,J.R., Philpot,R.M., Williams,D.E. and
            Ziegler,D.M.
  TITLE     The mammalian flavin-containing monooxygenases: molecular
            characterization and regulation of expression
  JOURNAL   Toxicol Appl Pharmacol 125 (1), 1-6 (1994)
   PUBMED   8128486
  REMARK    Review article
REFERENCE   10 (bases 1 to 2331)
  AUTHORS   Lawton,M.P., Cashman,J.R., Cresteil,T., Dolphin,C.T., Elfarra,A.A.,
            Hines,R.N., Hodgson,E., Kimura,T., Ozols,J., Phillips,I.R. et al.
  TITLE     A nomenclature for the mammalian flavin-containing monooxygenase
            gene family based on amino acid sequence identities
  JOURNAL   Arch Biochem Biophys 308 (1), 254-257 (1994)
   PUBMED   8311461
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314647.1 and BC035687.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001461.3.
            
            Summary: Metabolic N-oxidation of the diet-derived
            amino-trimethylamine (TMA) is mediated by flavin-containing
            monooxygenase and is subject to an inherited FMO3 polymorphism in
            man resulting in a small subpopulation with reduced TMA N-oxidation
            capacity resulting in fish odor syndrome Trimethylaminuria. Three
            forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult
            liver, and FMO3 are encoded by genes clustered in the 1q23-q25
            region. Flavin-containing monooxygenases are NADPH-dependent
            flavoenzymes that catalyzes the oxidation of soft nucleophilic
            heteroatom centers in drugs, pesticides, and xenobiotics.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.2479.1, Z47553.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254090.9/ ENSP00000254090.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1692              AK314647.1         228-1919
            1693-2331           BC035687.1         1532-2170
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.1"
     gene            1..2331
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /note="flavin containing dimethylaniline monoxygenase 5"
                     /db_xref="GeneID:2330"
                     /db_xref="HGNC:HGNC:3773"
                     /db_xref="MIM:603957"
     exon            1..53
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            54..225
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1692
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /EC_number="1.14.13.8"
                     /EC_number="1.6.3.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     dimethylaniline monooxygenase [N-oxide-forming] 5; FMO 5;
                     dimethylaniline oxidase 5; hepatic flavin-containing
                     monooxygenase 5; NAPDH oxidase; baeyer-Villiger
                     monooxygenase 1; flavin containing monooxygenase 5; NADPH
                     oxidase"
                     /codon_start=1
                     /product="flavin-containing monooxygenase 5 isoform 1"
                     /protein_id="NP_001452.2"
                     /db_xref="CCDS:CCDS926.1"
                     /db_xref="GeneID:2330"
                     /db_xref="HGNC:HGNC:3773"
                     /db_xref="MIM:603957"
                     /translation="MTKKRIAVIGGGVSGLSSIKCCVEEGLEPVCFERTDDIGGLWRF
                     QENPEEGRASIYKSVIINTSKEMMCFSDYPIPDHYPNFMHNAQVLEYFRMYAKEFDLL
                     KYIRFKTTVCSVKKQPDFATSGQWEVVTESEGKKEMNVFDGVMVCTGHHTNAHLPLES
                     FPGIEKFKGQYFHSRDYKNPEGFTGKRVIIIGIGNSGGDLAVEISQTAKQVFLSTRRG
                     AWILNRVGDYGYPADVLFSSRLTHFIWKICGQSLANKYLEKKINQRFDHEMFGLKPKH
                     RALSQHPTLNDDLPNRIISGLVKVKGNVKEFTETAAIFEDGSREDDIDAVIFATGYSF
                     DFPFLEDSVKVVKNKISLYKKVFPPNLERPTLAIIGLIQPLGAIMPISELQGRWATQV
                     FKGLKTLPSQSEMMAEISKAQEEIDKRYVESQRHTIQGDYIDTMEELADLVGVRPNLL
                     SLAFTDPKLALHLLLGPCTPIHYRVQGPGKWDGARKAILTTDDRIRKPLMTRVVERSS
                     SMTSTMTIGKFMLALAFFAIIIAYF"
     exon            226..414
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            415..577
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            578..720
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            721..920
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1273
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1346
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..2331
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2312..2317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /note="hexamer: AATAAA"
     polyA_site      2331
                     /gene="FMO5"
                     /gene_synonym="hBVMO1"
                     /note="major polyA site"
ORIGIN      
        1 gagtctcctc cagatcgcag ctgaaggatc tgttgagcgc ttcaggaaag gcggacaggc
       61 gacactaaca ggtgaagatc tcgggagacc atgactaaga aaagaattgc tgtgattggg
      121 ggaggagtga gcgggctctc ttccatcaag tgctgcgtag aagaaggctt ggaacctgtc
      181 tgctttgaaa ggactgatga catcggaggg ctctggaggt tccaggaaaa tcctgaagaa
      241 ggaagggcca gtatttacaa atcagtgatc atcaatactt ctaaagagat gatgtgcttc
      301 agtgactatc caatcccaga tcattatccc aacttcatgc ataatgccca ggtcctggag
      361 tatttcagga tgtatgccaa agaatttgac cttctaaagt atattcgatt taagaccact
      421 gtgtgcagtg tgaagaagca gcctgatttt gccacttcag gccaatggga agtggtcact
      481 gaatctgaag ggaaaaagga gatgaatgtc tttgatggag tcatggtttg cactggccat
      541 cacaccaatg ctcatctacc tctggaaagc ttccctggaa ttgagaagtt caaagggcag
      601 tacttccaca gtcgagacta taagaaccca gagggattca ctggaaagag agtcattata
      661 attggcattg ggaattctgg aggggatctg gctgtagaga ttagccaaac agccaagcag
      721 gttttcctca gcaccaggag aggggcttgg atcctgaatc gtgtagggga ctacggatat
      781 cctgctgatg tgttgttctc ttctcgactt acacatttta tatggaagat ctgtggccaa
      841 tcattagcaa acaaatattt ggaaaaaaag ataaaccaaa ggtttgacca tgaaatgttt
      901 ggcctgaagc ctaaacacag agctctgagt cagcatccaa ccttaaatga tgacctgcca
      961 aatcgtatca tttctggctt ggtgaaagtg aaaggaaatg tgaaggaatt cacggagaca
     1021 gctgccatat ttgaggatgg ctccagggag gatgacattg atgctgttat ctttgccaca
     1081 ggctatagct ttgactttcc atttctggaa gattccgtca aagtggtcaa aaacaagata
     1141 tccctgtata aaaaggtctt ccctcctaac ctggaaaggc caactcttgc aatcataggc
     1201 ttgattcagc ccttaggagc cattatgccc atttcagagc tccaaggacg ctgggccact
     1261 caggtattta aaggtctaaa gacattgccc tcacagagtg aaatgatggc agaaatatct
     1321 aaagctcaag aggaaattga caaaaggtat gtggagagcc aacgccatac cattcaggga
     1381 gactacatag ataccatgga agagcttgct gatttggtgg gggtcaggcc caatctgctg
     1441 tctctggcct tcactgaccc caagctggca ttacacttat tactgggacc ctgcactcca
     1501 atccactatc gtgtacaggg ccctggaaag tgggatgggg ctcgaaaagc tatcctcacc
     1561 acagatgatc gcatcaggaa gcctctgatg acaagagtag ttgaaaggag tagttctatg
     1621 acttcaacaa tgacaatagg caagtttatg ctagctcttg ccttctttgc tataattata
     1681 gcttacttct agttgtccta ttgtcactgc cctgtttttc attgggaagc ttatctacag
     1741 atgccttcag aatctgacga gattgactct cagtttcata ttgcccagaa atctacttta
     1801 atgtctcttt cgaaagcatt aattcacttt cctttttcct acaatgaaac ctgttttcca
     1861 tttgtattaa ctcatctccc ttccactcat gatccgtcac tcttccttgt ggtaatccct
     1921 agactgggag ctcaggtact cttttagtca tctttgtatg tctttagcag agttcttgac
     1981 atgtggtagg tgcttaataa atgtttgttg tttatcaaat tttatggtag ggagagtaag
     2041 tcagcatcgg tataaaatcg cttactccac gtaactcttc ttctgatagg gtttgatttt
     2101 ctattagaag ctcaatttta gttttttttc atattataac taaatatgtt tcctgagaga
     2161 taagagaaat aatgttccta caatagttgt atgtatctaa gataagacat atagatgctt
     2221 aagacatttt gtttcacttg ctattcacta gtgtacttga aacatggtca tttttagccc
     2281 ttttccttag gaaccatgtc tttattttct caataaagaa attactttca a
//
