LOCUS       NM_020066               6429 bp    mRNA    linear   PRI 09-JAN-2024
DEFINITION  Homo sapiens formin 2 (FMN2), transcript variant 2, mRNA.
ACCESSION   NM_020066 XM_371352
VERSION     NM_020066.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Yu Q, Zhang Y, Tian Y, Peng A, Cui X, Ding B, Yang L, Liu Y, Ju Y
            and Gao C.
  TITLE     Exosomal Circ_FMN2 Derived from the Serum of Colorectal Cancer
            Patients Promotes Cancer Progression by miR-338-3p/MSI1 Axis
  JOURNAL   Appl Biochem Biotechnol 195 (12), 7322-7337 (2023)
   PUBMED   36995659
  REMARK    GeneRIF: Exosomal Circ_FMN2 Derived from the Serum of Colorectal
            Cancer Patients Promotes Cancer Progression by miR-338-3p/MSI1
            Axis.
            Erratum:[Appl Biochem Biotechnol. 2023 Sep 27;:. PMID: 37755641]
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Huang S, Zhao J, Yu H and Chen G.
  TITLE     Mechanism of tumor-derived extracellular vesicles in prostatic
            cancer progression through the circFMN2/KLF2/RNF128 axis
  JOURNAL   Apoptosis 28 (9-10), 1372-1389 (2023)
   PUBMED   37452271
  REMARK    GeneRIF: Mechanism of tumor-derived extracellular vesicles in
            prostatic cancer progression through the circFMN2/KLF2/RNF128 axis.
REFERENCE   3  (bases 1 to 6429)
  AUTHORS   Li J, Peng T, Wang L, Long P, Quan R, Tan H, Zeng M, Wu X, Yang J,
            Xiao H and Shi X.
  TITLE     Heterozygous FMN2 missense variant found in a family case of
            premature ovarian insufficiency
  JOURNAL   J Ovarian Res 15 (1), 31 (2022)
   PUBMED   35227295
  REMARK    GeneRIF: Heterozygous FMN2 missense variant found in a family case
            of premature ovarian insufficiency.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6429)
  AUTHORS   Li DJ, Feng ZC, Li XR and Hu G.
  TITLE     Involvement of methylation-associated silencing of formin 2 in
            colorectal carcinogenesis
  JOURNAL   World J Gastroenterol 24 (44), 5013-5024 (2018)
   PUBMED   30510376
  REMARK    GeneRIF: FMN2 promoter hypermethylation may be an important early
            event in colorectal cancer, most likely playing a critical role in
            cancer initiation, and can serve as an ideal diagnostic biomarker
            in elderly patients with early-stage colon cancer.
REFERENCE   5  (bases 1 to 6429)
  AUTHORS   Fernandez-Barrera J and Alonso MA.
  TITLE     Coordination of microtubule acetylation and the actin cytoskeleton
            by formins
  JOURNAL   Cell Mol Life Sci 75 (17), 3181-3191 (2018)
   PUBMED   29947928
  REMARK    Review article
REFERENCE   6  (bases 1 to 6429)
  AUTHORS   McKnight AJ, Maxwell AP, Fogarty DG, Sadlier D and Savage DA.
  CONSRTM   Warren 3/UK GoKinD Study Group
  TITLE     Genetic analysis of coronary artery disease single-nucleotide
            polymorphisms in diabetic nephropathy
  JOURNAL   Nephrol Dial Transplant 24 (8), 2473-2476 (2009)
   PUBMED   19336575
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 6429)
  AUTHORS   Ryley DA, Wu HH, Leader B, Zimon A, Reindollar RH and Gray MR.
  TITLE     Characterization and mutation analysis of the human formin-2 (FMN2)
            gene in women with unexplained infertility
  JOURNAL   Fertil Steril 83 (5), 1363-1371 (2005)
   PUBMED   15866570
  REMARK    GeneRIF: It is likely that FMN2 has the same function as Fmn2 in
            the mouse, i.e., maintenance of the meiotic spindle.
            Identification of patients with meiosis I arrest is necessary to
            determine whether FMN2 mutations are a cause of unexplained
            infertility.
REFERENCE   8  (bases 1 to 6429)
  AUTHORS   Katoh M and Katoh M.
  TITLE     Characterization of FMN2 gene at human chromosome 1q43
  JOURNAL   Int J Mol Med 14 (3), 469-474 (2004)
   PUBMED   15289902
  REMARK    GeneRIF: FMN2 was characterized at human chromosome 1q43.
REFERENCE   9  (bases 1 to 6429)
  AUTHORS   Leader B, Lim H, Carabatsos MJ, Harrington A, Ecsedy J, Pellman D,
            Maas R and Leder P.
  TITLE     Formin-2, polyploidy, hypofertility and positioning of the meiotic
            spindle in mouse oocytes
  JOURNAL   Nat Cell Biol 4 (12), 921-928 (2002)
   PUBMED   12447394
REFERENCE   10 (bases 1 to 6429)
  AUTHORS   Leader B and Leder P.
  TITLE     Formin-2, a novel formin homology protein of the cappuccino
            subfamily, is highly expressed in the developing and adult central
            nervous system
  JOURNAL   Mech Dev 93 (1-2), 221-231 (2000)
   PUBMED   10781961
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL359918.43, DB476003.1,
            AF218941.1, DN990332.1, AF218942.2 and AF225426.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020066.4.
            
            Summary: This gene is a member of the formin homology protein
            family. The encoded protein is thought to have essential roles in
            organization of the actin cytoskeleton and in cell polarity. This
            protein mediates the formation of an actin mesh that positions the
            spindle during oogenesis and also regulates the formation of actin
            filaments in the nucleus. This protein also forms a perinuclear
            actin/focal-adhesion system that regulates the shape and position
            of the nucleus during cell migration. Mutations in this gene have
            been associated with infertility and also with an autosomal
            recessive form of intellectual disability (MRT47). Alternatively
            spliced transcript variants have been identified. [provided by
            RefSeq, Jul 2017].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 1. It encodes
            isoform 2 which is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319653.14/ ENSP00000318884.9
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AL359918.43        74062-74063
            3-491               DB476003.1         1-489
            492-1412            AL359918.43        74553-75473
            1413-1639           AF218941.1         1-227
            1640-1640           AL359918.43        75701-75701
            1641-2407           AF218941.1         229-995
            2408-4005           AL359918.43        189172-190769
            4006-4508           DN990332.1         8-510
            4509-6419           AF218942.2         7-1917
            6420-6429           AF225426.1         1941-1950
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q43"
     gene            1..6429
                     /gene="FMN2"
                     /note="formin 2"
                     /db_xref="GeneID:56776"
                     /db_xref="HGNC:HGNC:14074"
                     /db_xref="MIM:606373"
     exon            1..1842
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..5396
                     /gene="FMN2"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="formin-2 isoform 2"
                     /protein_id="NP_064450.3"
                     /db_xref="CCDS:CCDS31069.2"
                     /db_xref="GeneID:56776"
                     /db_xref="HGNC:HGNC:14074"
                     /db_xref="MIM:606373"
                     /translation="MGNQDGKLKRSAGDALHEGGGGAEDALGPRDVEATKKGSGGKKA
                     LGKHGKGGGGGGGGGESGKKKSKSDSRASVFSNLRIRKNLSKGKGAGGSREDVLDSQA
                     LQTGELDSAHSLLTKTPDLSLSADEAGLSDTECADPFEVTGPGGPGPAEARVGGRPIA
                     EDVETAAGAQDGQRTSSGSDTDIYSFHSATEQEDLLSDIQQAIRLQQQQQQQLQLQLQ
                     QQQQQQQLQGAEEPAAPPTAVSPQPGAFLGLDRFLLGPSGGAGEAPGSPDTEQALSAL
                     SDLPESLAAEPREPQQPPSPGGLPVSEAPSLPAAQPAAKDSPSSTAFPFPEAGPGEEA
                     AGAPVRGAGDTDEEGEEDAFEDAPRGSPGEEWAPEVGEDAPQRLGEEPEEEAQGPDAP
                     AAASLPGSPAPSQRCFKPYPLITPCYIKTTTRQLSSPNHSPSQSPNQSPRIKRRPEPS
                     LSRGSRTALASVAAPAKKHRADGGLAAGLSRSADWTEELGARTPRVGGSAHLLERGVA
                     SDSGGGVSPALAAKASGAPAAADGFQNVFTGRTLLEKLFSQQENGPPEEAEKFCSRII
                     AMGLLLPFSDCFREPCNQNAQTNAASFDQDQLYTWAAVSQPTHSLDYSEGQFPRRVPS
                     MGPPSKPPDEEHRLEDAETESQSAVSETPQKRSDAVQKEVVDMKSEGQATVIQQLEQT
                     IEDLRTKIAELERQYPALDTEVASGHQGLENGVTASGDVCLEALRLEEKEVRHHRILE
                     AKSIQTSPTEEGGVLTLPPVDGLPGRPPCPPGAESGPQTKFCSEISLIVSPRRISVQL
                     DSHQPTQSISQPPPPPSLLWSAGQGQPGSQPPHSISTEFQTSHEHSVSSAFKNSCNIP
                     SPPPLPCTESSSSMPGLGMVPPPPPPLPGMTVPTLPSTAIPQPPPLQGTEMLPPPPPP
                     LPGAGIPPPPPLPGAGILPLPPLPGAGIPPPPPLPGAAIPPPPPLPGAGIPLPPPLPG
                     AGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGI
                     PPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGIPPP
                     PPLPGAGIPPPPPLPGVGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGAGIPPPPPL
                     PRVGIPPPPPLPGAGIPPPPPLPGAGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGA
                     GIPPPPPLPGMGIPPAPAPPLPPPGTGIPPPPLLPVSGPPLLPQVGSSTLPTPQVCGF
                     LPPPLPSGLFGLGMNQDKGSRKQPIEPCRPMKPLYWTRIQLHSKRDSSTSLIWEKIEE
                     PSIDCHEFEELFSKTAVKERKKPISDTISKTKAKQVVKLLSNKRSQAVGILMSSLHLD
                     MKDIQHAVVNLDNSVVDLETLQALYENRAQSDELEKIEKHGRSSKDKENAKSLDKPEQ
                     FLYELSLIPNFSERVFCILFQSTFSESICSIRRKLELLQKLCETLKNGPGVMQVLGLV
                     LAFGNYMNGGNKTRGQADGFGLDILPKLKDVKSSDNSRSLLSYIVSYYLRNFDEDAGK
                     EQCLFPLPEPQDLFQASQMKFEDFQKDLRKLKKDLKACEVEAGKVYQVSSKEHMQPFK
                     ENMEQFIIQAKIDQEAEENSLTETHKCFLETTAYFFMKPKLGEKEVSPNAFFSIWHEF
                     SSDFKDFWKKENKLLLQERVKEAEEVCRQKKGKSLYKIKPRHDSGIKAKISMKT"
     exon            1843..2009
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2157
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2213
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..4147
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4148..4292
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4293..4380
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4381..4442
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4443..4534
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4535..4664
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4665..4811
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4812..4871
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4872..4992
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            4993..5085
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            5086..5137
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            5138..5287
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            5288..5369
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     exon            5370..6429
                     /gene="FMN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6411..6416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FMN2"
                     /note="hexamer: AATAAA"
     polyA_site      6429
                     /gene="FMN2"
                     /note="major polyA site"
ORIGIN      
        1 gcagccgcag ccgcagcgac ggcagccacg ggagccgccg cgcattatgc aaagcggcgg
       61 cagatgcgag cggggccagc cgggcgcgcg tcggcctccc ctcccagcgg ctccccccgc
      121 cgccgcctga ctctcccggg agactcccta ggcccggacc tggggccgag gagggccggg
      181 atggcctgag tgcccgcggc gcggcggcgc agcagcggga ttgcaccatg gggaaccagg
      241 atgggaagct gaagaggagc gcaggtgatg ctttgcacga aggcggcggt ggcgccgagg
      301 atgcgctggg gcccagggat gtggaagcca caaagaaggg gagcgggggc aagaaggcgc
      361 taggcaagca cggcaagggg ggagggggcg gcggcggcgg cggggagtcg ggcaagaaga
      421 agagcaagtc cgactccaga gcctcggtgt tttccaacct gcggatcagg aagaatctgt
      481 ccaaggggaa aggcgccggc ggctcccgcg aagatgtact ggattcccag gccctgcaga
      541 ccggggagct ggacagcgct cactccctgc tcaccaagac tccagacctc agcctctcgg
      601 cggacgaggc cggcctgtcg gataccgagt gtgcggaccc ttttgaggtg accggtccag
      661 ggggtcctgg gcctgccgag gctagggtcg ggggccggcc gatcgccgag gatgtggaaa
      721 ctgcagcagg ggcgcaggat ggacaaagga ccagctcggg ctcggacacg gacatctata
      781 gcttccattc ggctacggag caagaggatt tgctttcaga catccagcag gcgatccgcc
      841 tgcagcagca gcagcagcag cagctccagc tccagctcca gcaacagcag cagcagcagc
      901 agctccaggg cgccgaggag cctgcagcgc cccccactgc cgtctcccct cagcccgggg
      961 ccttcctggg cctggaccgg ttcctgctgg ggccgagcgg cggggctggg gaggccccgg
     1021 gcagtccgga caccgagcag gcgctgtccg cgctctccga cctgcccgag agcctggccg
     1081 ccgagccccg ggagccccag caaccgccgt cccccggcgg cctcccggtc tccgaggcgc
     1141 ccagtctccc ggcagcgcaa cccgcggcca aagactcgcc ctcctccacg gctttcccat
     1201 ttcccgaggc cgggccgggg gaggaagcgg ccggagcccc cgtgcgaggg gctggggaca
     1261 cggatgagga gggtgaggag gatgcttttg aggatgcgcc ccggggctct ccgggggagg
     1321 agtgggcccc ggaggtggga gaggacgccc cgcagaggct gggggaagag ccggaggagg
     1381 aggcgcaagg acctgacgcc cccgcggccg cttccctgcc cggcagcccc gcgcctagcc
     1441 agcgctgttt caagccctac ccgctcatca ccccctgcta catcaagacc accacccggc
     1501 agctcagctc gcccaatcac tccccgtctc agtcccctaa tcagagcccc aggatcaaga
     1561 ggcggccgga accctccctg agccgagggt ccagaactgc cctggcctcc gtagccgccc
     1621 cggccaagaa gcaccgggcc gacggcggcc ttgcggccgg cctgagccgc tcggctgact
     1681 ggacggagga gctaggcgcc cgcacgcccc gggtgggagg ctccgcgcac ctgctggagc
     1741 gcggggtggc gagtgacagc ggcggtgggg tgtccccagc actggccgcc aaggcgtctg
     1801 gggcccccgc ggctgcggat ggcttccaga acgtgttcac agggcgaacg ctgttggaga
     1861 agctgttcag ccagcaggag aacgggcctc cagaagaagc agagaagttt tgctcccgga
     1921 tcattgccat gggtcttctc cttcctttta gtgattgctt cagggaaccg tgtaatcaga
     1981 atgcccagac gaatgcagct tcgtttgatc aagatcaact ttatacctgg gctgcagtta
     2041 gtcaacccac acactcattg gactattcag aagggcagtt tcctaggcga gttccatcca
     2101 tggggccacc atccaaacct cccgatgagg aacacaggct cgaggatgct gaaacagaat
     2161 ctcaatctgc tgtttcagaa actccccaaa aacgctcaga tgctgtccag aaggaagttg
     2221 ttgacatgaa gtctgaggga caggccactg taattcagca gctggaacag actattgagg
     2281 atctgagaac caaaatagct gaactagaga ggcagtatcc tgccctggac acagaggtgg
     2341 ccagtggtca tcaagggctt gagaatggag tgacagcctc aggcgatgtc tgtctcgaag
     2401 ctctcaggtt agaagaaaag gaagtacggc atcataggat tttagaggcg aaatcgatac
     2461 agacttcccc cacggaagag ggcggggtgc tgacactgcc tcctgtggat gggctgccag
     2521 ggcgtcctcc atgcccccct ggggctgaaa gtggacctca gacaaagttc tgttcagaga
     2581 tttctttgat tgtgtctcca aggcgaatat cagtccagct cgacagccat cagcccacac
     2641 agagcatctc acagcctcca ccacctccat cccttctgtg gtctgctggg caaggacagc
     2701 ctgggtcaca gccgccccat tctatttcta ccgagtttca aaccagccac gaacactctg
     2761 tttcctctgc ctttaaaaac agctgtaaca tcccatctcc accacctctg ccttgcacag
     2821 agtcctccag ctccatgcct ggcctgggca tggtgcctcc cccacctccc cctctccctg
     2881 gcatgacagt gcctactctg cccagtacag ccattcccca acctcctcct ctgcagggta
     2941 cagaaatgct gccaccccct ccccctcctc ttcccggagc gggcatacct cctccgccgc
     3001 ctctacccgg agcaggcata ctccctctgc cccctctacc cggagcggga atacctcctc
     3061 cgccccctct acccggagcg gcaatacccc ctccgccccc tcttcccggg gcaggcatac
     3121 cccttcctcc ccctcttccc ggagcaggaa tacctcctcc accccctcta cccggagcgg
     3181 gcataccccc tcctccccca cttcccggag cgggcatacc ccctccgccc ccacttcccg
     3241 gagcgggcat accccctcct ccccctcttc ccggagcggg catacctcct ccaccccctc
     3301 tacccggagc gggcataccc cctccgcccc cacttcccgg agcgggcata ccccctccgc
     3361 ccccacttcc cggagcgggc ataccccctc ctccccctct tcccggagcg ggcatacctc
     3421 ctccaccccc tctacccgga gcgggcatac cccctccgcc cccacttccc ggagcgggca
     3481 tacccccacc tccccctcta cccggagcgg gcataccccc tccgccccct ctacccggag
     3541 tgggcatacc tcctccgccc cctctacccg gagcgggcat accccctcct ccccctctac
     3601 ccggagcggg cataccccct cctccccctc ttcccggagc gggcatacct cctccacccc
     3661 ctctacccag agtgggcata ccccctccgc ccccacttcc cggagcgggc atacccccac
     3721 ctccccctct acccggagcg ggcatacccc ctccgccccc tctacctgga gtgggaatac
     3781 ctcctccgcc ccctctacct ggagtgggaa tacctcctcc gccccctcta cctggtgctg
     3841 ggattccccc acctcctccc ttgccaggta tggggattcc acctgctcca gctcccccac
     3901 tccctccacc tgggacagga atcccaccgc cccctctgct tcctgtatca ggccctccac
     3961 tcctcccaca agttgggagt agcactttac caaccccaca ggtgtgtgga tttcttcctc
     4021 ctccattgcc aagtggcttg tttggattag ggatgaatca ggacaaaggg agtaggaagc
     4081 agcccataga gccttgtcga ccaatgaagc ctctttactg gaccaggatt caactacata
     4141 gtaaaagaga ctccagtact tcacttattt gggaaaaaat tgaagagcca tccatagatt
     4201 gtcatgaatt tgaggaatta ttttctaaaa ctgctgtaaa ggagagaaag aaacctatct
     4261 ctgatactat ctcaaagacg aaggctaaac aagttgtcaa gttattaagc aacaaaagat
     4321 cacaagcagt tggaatacta atgtctagcc ttcatttaga tatgaaagac atacaacatg
     4381 ctgttgtgaa cttggataat tctgtggttg acctggagac ccttcaagct ctctatgaga
     4441 atagagcaca gtcagacgaa ctcgaaaaaa tagaaaagca tggccgatct tccaaagaca
     4501 aggaaaatgc caagtctctg gacaaacctg aacagttcct ttatgaactg tcactaatcc
     4561 ccaacttttc agagcgagtc ttttgcatcc tgttccagtc cacattttca gaaagcattt
     4621 gctcaattcg tcgcaaactg gaattactac agaaattgtg tgagacatta aaaaatggcc
     4681 caggggttat gcaggttcta ggtttggttc ttgcctttgg caactacatg aatggaggaa
     4741 ataagactcg aggacaggca gatggctttg gattagacat tcttccaaaa ctgaaagatg
     4801 tcaagagcag tgacaatagc agaagccttt tgtcatatat tgtttcgtat tatctccgaa
     4861 attttgatga ggatgctgga aaagaacagt gcctctttcc actgccagaa ccccaggacc
     4921 tttttcaggc ctcacagatg aagtttgaag attttcaaaa agatctcaga aaactgaaga
     4981 aagacttgaa agcctgtgaa gttgaagcag ggaaagtata ccaggtctcc tcaaaagagc
     5041 atatgcagcc tttcaaggaa aacatggaac aatttattat tcaagccaaa attgaccaag
     5101 aggcagagga aaattcactg acagagactc ataaatgctt tttggagacc acggcatatt
     5161 tcttcatgaa accaaaactt ggagagaagg aggtgtcccc aaatgctttc ttcagtatct
     5221 ggcatgaatt cagctctgac tttaaagact tctggaagaa agagaacaaa cttcttctac
     5281 aagagagagt aaaagaagcc gaagaggtgt gtagacagaa gaaaggaaaa tcactttata
     5341 aaataaaacc aagacatgac tctggaatta aagcaaagat aagcatgaaa acttgaacaa
     5401 tgaaaagcag aatgaaaatg agtcattgca acgactttca caaaattcag ctgacctgag
     5461 agtgggaggg aaactaccgt cattctgctc atgtttcttc ttgacctctt gcataatctt
     5521 tttgttttct agacagttca ctaattgttg aattttactg tatattcata taaaaatgca
     5581 aacgtactag accagtggag aatttgacac cttttctttt tgtaaaagtt tatggtatta
     5641 taccgataga ccaaaacagc atgtgtaaga ggcagtatct gcactaattc tcaacatgct
     5701 aaacattaac tacaattcac tgttgtgaga atattcctcg tcacagcaaa aacactttcc
     5761 tttctactga caaccagtcc tccacatcac agcatttaga catatgggta aaatgttatt
     5821 tctagtgaat tgtttgtatc agtttcatgt ctaagtataa attttctatt ttaaaattta
     5881 agaaccgttt ataatcagtg ctttcccaac tcttgggttg ctctccataa ctatgtattt
     5941 gtgaaagaaa atggtcattt tttttactga agtcatataa tgacttgggt cagctcgtaa
     6001 tgcattgtga tggttttgta tgagctgggt gtttttttcc attactttta atgatcttcg
     6061 ttgcaagtta tagttgtgga taaaggggag aatttattgc tcttgcaaac caattatgga
     6121 aagcaactta agaaaaccaa tgttctaaat cataattgtt tgtatttatg taaagtatgg
     6181 tctcttactt tttagtttgt agtttaagtg caaagaaaca gtagtggttt tttttctatt
     6241 gttttgtagt cttcctgtcc ccttcagtcc ctccagtgtg tatattacca ttctccaatg
     6301 aaataatagg gcatttaaca aagatcgcta tgtgcaatac tgtatttagt gtttctattt
     6361 caatttttct aggatgttaa tttatatgaa aataaaatga ataataaaag aataaagata
     6421 cttgcaaaa
//
