LOCUS       NM_001277313           13511 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens formin 1 (FMN1), transcript variant 1, mRNA.
ACCESSION   NM_001277313
VERSION     NM_001277313.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13511)
  AUTHORS   Zhao,L., Liu,H., Luo,S., Moorman,P.G., Walsh,K.M., Li,W. and Wei,Q.
  TITLE     Associations between genetic variants of KIF5B, FMN1, and MGAT3 in
            the cadherin pathway and pancreatic cancer risk
  JOURNAL   Cancer Med 9 (24), 9620-9631 (2020)
   PUBMED   33200553
  REMARK    GeneRIF: Associations between genetic variants of KIF5B, FMN1, and
            MGAT3 in the cadherin pathway and pancreatic cancer risk.
REFERENCE   2  (bases 1 to 13511)
  AUTHORS   Watanabe,N., Tohyama,K. and Yamashiro,S.
  TITLE     Mechanostress resistance involving formin homology proteins: G- and
            F-actin homeostasis-driven filament nucleation and helical
            polymerization-mediated actin polymer stabilization
  JOURNAL   Biochem Biophys Res Commun 506 (2), 323-329 (2018)
   PUBMED   30309655
  REMARK    GeneRIF: In the article it is discussed how and to what extent
            formins-mediated F-actin restoration might confer mechanostress
            resistance to the cell. (Review)
            Review article
REFERENCE   3  (bases 1 to 13511)
  AUTHORS   Rana,M.K., Aloisio,F.M., Choi,C. and Barber,D.L.
  TITLE     Formin-dependent TGF-beta signaling for epithelial to mesenchymal
            transition
  JOURNAL   Mol Biol Cell 29 (12), 1465-1475 (2018)
   PUBMED   29668357
  REMARK    GeneRIF: These findings reveal a previously unrecognized role for
            formin-dependent actin architectures in proximal TGF-beta signaling
REFERENCE   4  (bases 1 to 13511)
  AUTHORS   Isogai,T. and Innocenti,M.
  TITLE     New nuclear and perinuclear functions of formins
  JOURNAL   Biochem Soc Trans 44 (6), 1701-1708 (2016)
   PUBMED   27913680
  REMARK    GeneRIF: Formin-1 (FMN1) was shown to reside in the nucleus and
            studies indicate that some formins can shuttle in and out of the
            nucleus.
            Review article
REFERENCE   5  (bases 1 to 13511)
  AUTHORS   Murugesan,S., Hong,J., Yi,J., Li,D., Beach,J.R., Shao,L.,
            Meinhardt,J., Madison,G., Wu,X., Betzig,E. and Hammer,J.A.
  TITLE     Formin-generated actomyosin arcs propel T cell receptor
            microcluster movement at the immune synapse
  JOURNAL   J Cell Biol 215 (3), 383-399 (2016)
   PUBMED   27799367
  REMARK    GeneRIF: actin arcs populating the medial, lamella-like region of
            the immunological synapse (IS) arise from linear actin filaments
            generated by one or more formins present at the IS distal edge.
REFERENCE   6  (bases 1 to 13511)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of the human FMN1 gene in
            silico
  JOURNAL   Int J Mol Med 14 (1), 121-126 (2004)
   PUBMED   15202026
REFERENCE   7  (bases 1 to 13511)
  AUTHORS   Kobielak,A., Pasolli,H.A. and Fuchs,E.
  TITLE     Mammalian formin-1 participates in adherens junctions and
            polymerization of linear actin cables
  JOURNAL   Nat Cell Biol 6 (1), 21-30 (2004)
   PUBMED   14647292
REFERENCE   8  (bases 1 to 13511)
  AUTHORS   Tanaka,K.
  TITLE     Formin family proteins in cytoskeletal control
  JOURNAL   Biochem Biophys Res Commun 267 (2), 479-481 (2000)
   PUBMED   10631086
  REMARK    Review article
REFERENCE   9  (bases 1 to 13511)
  AUTHORS   Zeller,R., Haramis,A.G., Zuniga,A., McGuigan,C., Dono,R.,
            Davidson,G., Chabanis,S. and Gibson,T.
  TITLE     Formin defines a large family of morphoregulatory genes and
            functions in establishment of the polarising region
  JOURNAL   Cell Tissue Res 296 (1), 85-93 (1999)
   PUBMED   10199968
  REMARK    Review article
REFERENCE   10 (bases 1 to 13511)
  AUTHORS   Maas,R.L., Jepeal,L.I., Elfering,S.L., Holcombe,R.F., Morton,C.C.,
            Eddy,R.L., Byers,M.G., Shows,T.B. and Leder,P.
  TITLE     A human gene homologous to the formin gene residing at the murine
            limb deformity locus: chromosomal location and RFLPs
  JOURNAL   Am J Hum Genet 48 (4), 687-695 (1991)
   PUBMED   1673046
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY009284.1, AC055874.8,
            AC090098.7, AI040235.1, AK127078.1, AC018515.7, AC090877.4 and
            BM663620.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 12, 2019 this sequence version replaced NM_001277313.1.
            
            Summary: This gene belongs to the formin homology family and
            encodes a protein that has a role in the formation of adherens
            junction and the polymerization of linear actin cables. The
            homologous gene in mouse is associated with limb deformity.
            Alternatively spliced transcript variants have been found for this
            gene. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). The exon
            combination of this variant is inferred based on partial human and
            full-length orthologous transcript alignments.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.1379021.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000616417.5/ ENSP00000479134.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                HY009284.1         21-38
            19-137              AC055874.8         138426-138544
            138-289             AC055874.8         139062-139213
            290-354             AC090098.7         33096-33160         c
            355-653             AC090098.7         7645-7943           c
            654-2199            AC090098.7         6099-7644           c
            2200-2683           AI040235.1         1-484               c
            2684-3154           AK127078.1         474-944
            3155-3155           AC018515.7         90193-90193
            3156-5006           AK127078.1         946-2796
            5007-13418          AC090877.4         100147-108558       c
            13419-13511         BM663620.1         17-109              c
FEATURES             Location/Qualifiers
     source          1..13511
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q13.3"
     gene            1..13511
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /note="formin 1"
                     /db_xref="GeneID:342184"
                     /db_xref="HGNC:HGNC:3768"
                     /db_xref="MIM:136535"
     exon            1..137
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            138..289
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            290..354
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            355..2352
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     CDS             486..4745
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /note="isoform a is encoded by transcript variant 1;
                     formin (limb deformity); limb deformity protein homolog"
                     /codon_start=1
                     /product="formin-1 isoform a"
                     /protein_id="NP_001264242.1"
                     /db_xref="CCDS:CCDS61581.1"
                     /db_xref="GeneID:342184"
                     /db_xref="HGNC:HGNC:3768"
                     /db_xref="MIM:136535"
                     /translation="MEGTHCTLQLHKPITELCYISFCLPKGEVRGFSYKGTVTLDRSN
                     KGFHNCYQVREESDIISLSQEPDEHPGDIFFKQTPTKDILTELYKLTTERERLLTNLL
                     SSDHILGITMGNQEGKLQELSVSLAPEDDCFQSAGDWQGELPVGPLNKRSTHGNKKPR
                     RSSGRRESFGALPQKRTKRKGRGGRESAPLMGKDKICSSHSLPLSRTRPNLWVLEEKG
                     NLLPNGALACSLQRRESCPPDIPKTPDTDLGFGSFETAFKDTGLGREVLPPDCSSTEA
                     GGDGIRRPPSGLEHQQTGLSESHQDPEKHPEAEKDEMEKPAKRTCKQKPVSKVVAKVQ
                     DLSSQVQRVVKTHSKGKETIAIRPAAHAEFVPKADLLTLPGAEAGAHGSRRQGKERQG
                     DRSSQSPAGETASISSVSASAEGAVNKVPLKVIESEKLDEAPEGKRLGFPVHTSVPHT
                     RPETRNKRRAGLPLGGHKSLFLDLPHKVGPDSSQPRGDKKKPSPPAPAALGKVFNNSA
                     SQSSTHKQTSPVPSPLSPRLPSPQQHHRILRLPALPGEREAALNDSPCRKSRVFSGCV
                     SADTLEPPSSAKVTETKGASPAFLRAGQPRLVPGETLEKSLGPGKTTAEPQHQSPPGI
                     SSEGFPWDGFNEQTPKDLPNRDGGAWVLGYRAGPACPFLLHEEREKSNRSELYLDLHP
                     DHSLTEQDDRTPGRLQAVWPPPKTKDTEEKVGLKYTEAEYQAAILHLKREHKEEIENL
                     QAQFELRAFHIRGEHAMITARLEETIENLKHELEHRWRGGCEERKDVCISTDDDCPPK
                     TFRNVCVQTDRETFLKPCESESKTTRSNQLVPKKLNISSLSQLSPPNDHKDIHAALQP
                     MEGMASNQQKALPPPPASIPPPPPLPSGLGSLSPAPPMPPVSAGPPLPPPPPPPPPLP
                     PPSSAGPPPPPPPPPLPNSPAPPNPGGPPPAPPPPGLAPPPPPGLFFGLGSSSSQCPR
                     KPAIEPSCPMKPLYWTRIQISDRSQNATPTLWDSLEEPDIRDPSEFEYLFSKDTTQQK
                     KKPLSETYEKKNKVKKIIKLLDGKRSQTVGILISSLHLEMKDIQQAIFNVDDSVVDLE
                     TLAALYENRAQEDELVKIRKYYETSKEEELKLLDKPEQFLHELAQIPNFAERAQCIIF
                     RSVFSEGITSLHRKVEIITRASKDLLHVKSVKDILALILAFGNYMNGGNRTRGQADGY
                     SLEILPKLKDVKSRDNGINLVDYVVKYYLRYYDQEAGTEKSVFPLPEPQDFFLASQVK
                     FEDLIKDLRKLKRQLEASEKQMVVVCKESPKEYLQPFKDKLEEFFQKAKKEHKMEESH
                     LENAQKSFETTVRYFGMKPKSGEKEITPSYVFMVWYEFCSDFKTIWKRESKNISKERL
                     KMAQESVSKLTSEKKVETKKINPTASLKERLRQKEASVTTN"
     exon            2353..2528
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2646
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2708
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            2709..3472
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            3473..3623
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            3624..3711
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            3712..3773
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            3774..3862
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            3863..3992
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            3993..4139
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4140..4199
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4200..4320
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4321..4413
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4414..4465
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4466..4615
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4616..4700
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     exon            4701..13511
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      13489..13494
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /note="hexamer: AATAAA"
     polyA_site      13511
                     /gene="FMN1"
                     /gene_synonym="FMN; LD"
                     /note="major polyA site"
ORIGIN      
        1 cctttgggct ttggctctgg actggagcgc agcatccttc gaggctgcag ccgccacggc
       61 tgttgccgta agcaatcctc ctgcctcagc ctcccgagta gctgggactc gggaccagcc
      121 tcccgagtat tggtcagatg acatttacca tcaccgtttt cttcttcttt catcatcatc
      181 atcatcacca ttgccactac caccacctcc aaggccacta ccaccacagc atctctgttc
      241 ctccatcaga gctcttcaag ttctgtaaag gcagattctc taaaggcatg cctggtggca
      301 tcacgttttt cacatctccg catctggacc ctgcccaagc gaaggctgat ctaggcagtc
      361 tcattgctgg agatgactgt cagctgcttt ctctgcatag ccaccatcac atgcctcacc
      421 actggagcct gggaagcctg accacaaacc ggcatctcca aggcatgaat aattaggtag
      481 gcataatgga aggcactcat tgtaccctcc aattgcataa gcccattacg gaactctgct
      541 acatcagctt ctgtcttcca aagggggaag tcagaggatt ttcatacaag ggcactgtaa
      601 ctctagacag atccaataaa ggttttcata actgctacca agtcagggag gagtcagaca
      661 tcatcagcct cagccaggag ccggacgaac atccaggcga catatttttc aagcagactc
      721 ccacgaaaga cattctaact gagctgtaca aactcacaac agagagggag agactgctaa
      781 ccaatctcct gagctcagac cacatcctgg ggatcacgat ggggaaccag gaggggaagc
      841 tgcaagagct gtccgtgagc ctggcccccg aggatgactg tttccagagt gctggtgact
      901 ggcagggaga gctccccgtg ggccctctca ataagaggag cacccacggg aacaaaaagc
      961 ctcggaggtc tagtggaagg agagagagct ttggggccct tccacagaag aggaccaaaa
     1021 gaaaagggcg tggaggccga gaatcagctc ctctgatggg caaggacaag atctgttcca
     1081 gccactccct tcctctttct agaacaaggc ctaacctttg ggtactagag gagaaaggaa
     1141 atctgctccc gaatggggca cttgcctgct ccctgcagag gagagagagc tgccccccag
     1201 atattcccaa gacgccagac acagaccttg gctttgggag ctttgagacg gctttcaagg
     1261 acactgggct tggaagagaa gtgctgcccc ctgactgcag ctccacagag gcaggagggg
     1321 atggcattcg gaggccgccg agcgggctgg agcatcagca aacaggtttg tctgaaagtc
     1381 accaggaccc tgagaagcat ccagaggcag aaaaggatga gatggagaag ccggctaagc
     1441 ggacttgcaa gcagaaacct gtctccaaag tggtggccaa agttcaggac ctgtcctccc
     1501 aggtacaaag agtagttaaa acgcattcta agggtaagga gacgattgcc attcgcccag
     1561 cagcccacgc tgagtttgta cccaaagccg acttgctcac cctcccggga gctgaggctg
     1621 gggctcatgg ctccaggcgg cagggcaagg agcggcaagg ggataggtca tcgcagtcgc
     1681 cagccgggga aacagcctcc atttctagtg tgtcggccag tgccgagggg gccgtgaaca
     1741 aggtccccct gaaggtgata gagagtgaga agttagatga agcccctgag gggaaaagac
     1801 tgggcttccc tgtccacacg agtgtccctc acactcgccc agaaacgaga aacaagagga
     1861 gagccgggtt gccccttggt ggccacaagt ccttgtttct ggatctgccc cacaaagtag
     1921 gtcctgactc ctcacaaccc agaggtgata agaagaagcc atccccacca gcaccggcag
     1981 ctcttggcaa ggtgtttaat aattcagcct cgcagtccag cacacacaaa cagacgtcac
     2041 ctgttccctc gcctctgtct ccaaggctcc ccagccctca gcagcatcac aggatcctcc
     2101 ggctccctgc attgcctggt gagagggaag ctgctcttaa tgactctcct tgtagaaaga
     2161 gccgtgtctt ctctgggtgc gtctctgctg acaccttgga gccaccatcc tctgcaaagg
     2221 tcacggagac caaaggagcc agcccggcct tcctcagagc aggccaacct cggttggtgc
     2281 ctggggaaac tttggaaaag agcttggggc cagggaagac cacagctgag ccccagcacc
     2341 agtcacctcc aggtatctcc tctgagggct ttccctggga cggcttcaat gagcagacac
     2401 ctaaagacct tcccaacaga gatggaggcg cgtgggttct gggctacaga gcgggaccag
     2461 cctgtccatt tttgcttcat gaggaaaggg agaagtcaaa caggagcgaa ttgtacttgg
     2521 atctccatcc tgaccacagc ctgactgagc aggatgacag gactcctggc agacttcaag
     2581 ctgtctggcc acccccaaag acaaaagaca cagaagaaaa agtgggactg aagtacactg
     2641 aagcagaata ccaagctgct attttacact tgaagaggga gcacaaagaa gaaattgaaa
     2701 acctgcaggc acagtttgaa cttcgggcat ttcatatccg gggcgagcat gcaatgataa
     2761 cagcgagact agaagaaacc attgaaaatc tgaaacacga gctagaacac agatggcgag
     2821 ggggttgtga agagaggaaa gatgtgtgca tttccaccga tgatgactgc cctccaaaga
     2881 ccttcagaaa tgtgtgcgtc cagacagaca gagagacctt cctcaagccc tgtgaaagtg
     2941 aaagcaagac aaccagaagt aatcaattag tacccaaaaa gctgaatatc tcctctttaa
     3001 gccagctctc acccccaaat gaccacaaag acatccatgc agcactccag ccaatggagg
     3061 gcatggcatc aaatcagcag aaggcattgc ctccgcctcc cgcatccatc cctccccctc
     3121 cgcccctccc ctcaggactt ggatctttgt ctcccgcacc tccaatgcca cctgtgagtg
     3181 ctgggccacc gctaccacct ccgcctcctc caccgccacc tctacctcca ccttcaagtg
     3241 ctggacctcc accaccccca cccccacccc cacttcctaa ctcccctgcc ccacccaacc
     3301 ctggagggcc tcctcctgct ccaccacccc caggacttgc acccccacct ccccctggac
     3361 tcttctttgg acttggctct tcttccagtc aatgtcctcg aaaaccagcc atcgagccca
     3421 gttgtcccat gaagccttta tattggacta ggatacaaat aagtgatagg agccaaaatg
     3481 ctacaccaac cttatgggac tccttagaag aacctgacat tcgggacccc agtgaatttg
     3541 agtatttatt ctccaaagac acaactcaac agaagaaaaa acctctgtca gagacttatg
     3601 agaagaaaaa caaggtcaaa aagatcatca aattgttgga tggaaaacga tctcaaactg
     3661 tgggaatctt gatatctagt ttacatttag aaatgaagga tatccaacag gccattttca
     3721 atgtggatga ctccgtggtt gatctggaga ccctggcagc cttatatgaa aacagagccc
     3781 aagaggatga gctggttaaa ataagaaagt attacgagac atccaaagaa gaagaactga
     3841 agctgctgga taaacctgag caatttttac atgagttagc ccagattcct aattttgctg
     3901 aacgtgccca gtgcataatc ttcagatctg tcttttctga gggtatcacc tccttgcaca
     3961 gaaaggtaga gatcatcacg cgagcttcta aggacttgct gcacgtgaag agcgtgaagg
     4021 atattttagc tctcatcttg gcttttggaa attatatgaa tggaggaaat aggactcggg
     4081 gacaagccga tggatatagc ttagaaattc tgcccaaact caaggatgtc aaaagtcggg
     4141 ataatgggat taatctggtg gactacgttg ttaagtatta cctgcgttac tatgatcagg
     4201 aagctggaac agaaaagagt gttttcccct tgccggaacc acaggatttc tttctggcct
     4261 cccaagtcaa gtttgaagac ctcataaaag atttgagaaa actgaagagg caactagaag
     4321 caagtgagaa acagatggtg gtggtgtgca aggagtcccc aaaggagtat ctccagcctt
     4381 tcaaggacaa actagaggag ttcttccaaa aagccaaaaa agagcataag atggaagaaa
     4441 gtcacttgga gaatgcacag aaaagttttg aaacaacagt acgatatttt gggatgaagc
     4501 caaagtctgg tgagaaggag atcacaccca gctacgtgtt tatggtgtgg tatgagttct
     4561 gcagtgactt caagacaatt tggaaacggg agagtaaaaa catatctaaa gaaagattga
     4621 aaatggctca ggaatcagtc agcaagttga cttcagagaa gaaagtggag acaaagaaaa
     4681 tcaatcccac tgctagcctg aaagaaagac tgcgtcagaa ggaagccagt gtgaccacta
     4741 actaagatga agacacatgg aaatgatggc actggaggtg gaggaccttg cacgcatact
     4801 ctttgtgacc acagggttgc aggacgttct tgaaagatgt gtcactaaat gtttgttttt
     4861 gctcatctct ttctgaggtc atctgcagag agtgccccat gccttcttta agaagtccct
     4921 cattaagccg caggaacaat ggaaaactat ttaagggaac attgcagaaa tatttgatga
     4981 ctgtttcttg tggaagccca aagtccactc taagagcaga agaaatacca aaatgtttcc
     5041 aaaatttttt taaaagctga gatttccagc tttataacca aagcttgata tatgtcacat
     5101 tgtcacagaa gagagaaaag atcattgagg acagttgcct tgggagagtt caagtctttg
     5161 tcgttacaca ctgctgtttt gattattggt cttagttttg atcctgttgc agcaaaatcc
     5221 tgcagcatct ttctctccaa taatgttgca actcaccaaa actattcttg aagaggtcca
     5281 agaacatgta ttatccagac taaaaaaatg attttttgtt gttatggttt acgtgatgaa
     5341 aaggagaaaa aacagaatca tacctgggtg gagagaggga gacaaatagc cataaacttc
     5401 atcctggaga acaagttacc atgcaaggag ttgacatcag ttgttaagtg aggcccattc
     5461 ttgtttttac atcggtcctt tgtggttttt ctgggccagc agagattctg caccccaact
     5521 cccaggagaa aatgtaatac ctgagcaagc acagccttgg gtgtgcttgg acaagagacc
     5581 cagcggcaga gcacctttta cattttggac cgttcatgaa tgggcaatgt tgtgttgata
     5641 cgcagccgag ttctacgtta tgtgctgtga ctgtgcagct ggacgacccc catccccagg
     5701 aggcagtttc cagacaggag agcaaatgca actcacagca cctgctcatg accttggctg
     5761 ataggcatgt gaagtaggat gaagcaggac tctttaatgt caaaaaaact gaagggcaga
     5821 aaaaaggttc cttacatcaa aatgaaagta attacattaa tagaaaactt ccaagtggaa
     5881 tattgttcac aggcagctct ttctgaccct ggctcttgag tcacataagg aaaccacctt
     5941 tgacctctct gacacttttg tctttaagcc acgtctctga aatcttgtga ggggtgaaga
     6001 aagaggactg gagatgaatg gacaatatga tcaaagactc atttttaggc cttgaagagg
     6061 ccgagctttc tccccccaat tggtggagga cagtccagag atgagcagaa atcttaaatt
     6121 ccttcctgat ccagcctcca catgtggcct gccccttgcc ccagcatctt tccagaaccc
     6181 ctggacttgc caggtgcctg agcatcttcc caggatatgc ctgccagcca gcagcccctc
     6241 aggaatgctt ctataataga tccagtaatg aaagcagcac catatcctaa tctaatgtca
     6301 gcttcgagaa tgaagactgc agtcaggatc aaatcaagtc acagaagcca tattcatgca
     6361 ggtgctaacc gccttttcta ctggtgcaac tgaaaggaat atggtaacat ggttggattt
     6421 ttaaaattca caatgaagga acgagacaag caaaacagaa aagccgagaa ggaacatgca
     6481 tgactaaaaa ggcttgaagt tgcttgtgag caagaagctt tttatgctgc tttcttacat
     6541 agtttctctt cattttcact taactctgaa gacaaccaaa agaacggatc aattaaaaac
     6601 tgattccact tataaccatt gtatgctcct tctagtgtta tcatgcacaa gaaaagtgcc
     6661 agttttaccc atgccatata attcagctct tttgagttat gtggggccag cagaagtctc
     6721 aatccttata ggcttgatgg tgagagcagc gggaggtttg aggtgtactc attgcagtcc
     6781 actttggtct gtaagtagga tgcttacgag aaatcagtga aggtaagcag ggctgccact
     6841 gtggccacgt aacccttcag gaaccagtgc tgaagaataa tgagtgagaa cacttaattt
     6901 gagagttaga caaatacagc ccactttgga tttcatccta gtgaacagac taaaactttg
     6961 cagtaaaata cctatttcta caaagcaagg tttgcctgcc tcggttgggg aaacctagtg
     7021 gttttgtcac actagtcgat gtatgatgtt agtgttgtaa gacaccatgg cagaatcttc
     7081 attattctca cctattctga accctgcatc atgtatctgg gatgccccct cccaagtcca
     7141 ccaatgtacc acgttgctga cacagccttc atttttaact caaattgatg ctcagtaaag
     7201 gtcaatgaca atttgttcaa ggaagttcat ctaaaataat ctggaggcct tgaaaatgat
     7261 ccctctaaaa tgctgttgag cttgtgttca gtgtagcagg aggatctgaa cttttcctgg
     7321 agaggaggtg atcgggatgc catttggcag cttaagcaca tcctgccagc aggctttaag
     7381 tggctgttct aggcatagcc ctccctgaac tagaaaggag agtaaccagt ttgctatgct
     7441 gcatctaacc agacacacct gacttagtag taactctaag aacccttaac ttacaaacaa
     7501 ctttgtctcc tcttagacaa tattcattaa atctgaggac acagtttgac agtagttcag
     7561 attcttgcat gagcaattga cagcatcaga gcacacaata tcataagtta cataaattac
     7621 acaaagtaca tcataaatta cactacagac acgttaggta aaaagcatca ggcccgggcc
     7681 gggcgtggtg gctcatgcct ataatcccag cactttggga ggccaagcgg gggcggatca
     7741 cgaggtcagg agattgagac catcctggct aacacggtga aaccccatct ctactaaaaa
     7801 tacaaaaaat tagccgggcg tggtggtggg cgcctgtagt cccagctact cgggaggctg
     7861 aggcaggaga atggcgtgaa cccaggaggt ggagcttgca gctagccgag atagcaccac
     7921 tgcactccag cctgggcgac agtgagactc cgtttcaaaa aaaaaagcat caggcccatg
     7981 aagtatcaaa aaccaggtta ggaggtgtgc tgtatggctt ttatctcaat aagtggcagc
     8041 ttactgggga aaaagaaata agaaaaagtg tggccaagca gaagtggatg tttatgatga
     8101 cggtgggggc ccagaggtca taggtgatgg tcctactgcc caacagttgc cttttgaatc
     8161 cttatgttga tgatggtgtc tttctacaca ctcactgcaa ctactcagga attataaaga
     8221 ttttctgaaa tctgagaagt ggaataagga aacccagtgg agctattaca cttttaaatg
     8281 ttctagccaa aacaaattgc cagtatggtt gaggaattga atatcagatg gcatgcagtc
     8341 tcccctcccc tcccctatca aggacagtag aagctgcaaa cctcttctca gcctccttca
     8401 ggcacctcct gttttcataa gcaccatgaa gaatggtgtt tcctaaatga aaagttcaga
     8461 tactgctggt aaagaaactg gaaaaactga aggaaggaat aaaatatgtt ggtggtatta
     8521 acacctccag taagataagt cattggtacg gtttttctaa gggccgcagt gagatgtagc
     8581 agaagaaata tgtttatttt gggcaaagat ttgcttattg gcctgggatt gcacattttc
     8641 tctatgatgt ttgcaacaga gaaattttca ttttaatgat actcttcctt tcttgaaagt
     8701 tacatgtcct catttctgtt tctgcttctc ccttggccag tgtcattgat gcagatcatt
     8761 gcatacaaca gcacagcaaa ttgcgccaag tcagtgtgtg aatgggaatt agcaatgcac
     8821 gcttgcaggc tctttttcca ggaaccaggc tgtaacattg accggggaac ctcttcattc
     8881 cctgacatac taaattggtc aattttgtag tactcatctc catccacaaa aacaacaaag
     8941 ataaaatcaa tttggagctt tatatgacaa aaaggaagaa gtccaaaaat atatagcctt
     9001 cccccctccc acatatctga tccaaatatt aaatatctga ttttataaaa cagtttaaat
     9061 tgtcttttta aattgctaac aagcatcagt tatatttgag cttcattttt cttttatcct
     9121 gtctgtccta ataaaaacaa aaatggccaa aaagtggaga ggaaagaaaa agcttccact
     9181 ttttaaaatt tgttttgtat tagttatttt acctttaggg accctacaac tgaagagagt
     9241 ggagagaata aagaagtgaa ctttcattca tgtctgctac tttgacctca taaagaaatc
     9301 tgcaacacag atgaaaccag tctgtgtggc caagctgatg ggatttgaaa agagtggata
     9361 atttttagcc tttccaaaat gcaaataact tggatatatt tccattttcc agctatagag
     9421 agaaacaatc cagctgcctg gaaactgtta ttagtataga agtggcttaa agaaaagcat
     9481 cttcaaatac ttgacttagc atatctttct tctttagact tacataatga tattcatctc
     9541 tccattagca attgaatcca ggcataaccg ggctcagtta cacagcttgt ctgtgactgg
     9601 gaagcccatt ttgtgtcatt tctgcacacc tgtccgctgt tgacacctta ttagaggcac
     9661 tgcattttgg tttcacaatt cttcatctcc cttcccaagg aatgggcaat ctactgaact
     9721 tccagatcaa tgctttgttg gaaagtgatg aatttaagag agttttaaca tgtcaaattt
     9781 gaagccaatt cagtatcctt atttgttcca agactcttaa ccaatactgt gatataaagt
     9841 taggatttgg gaaacgtgta gttaacaact tggaaacatg aagatattaa aatggcagct
     9901 ggctaactgt ctcttaagcc actatgcaat ttctaccaat ggagagatta gggaagcaaa
     9961 ttaagcctca ttcagtaggt ctgctgtctg gtggaactta cggaactcat ttcctcctct
    10021 atccccagcc cttctcctaa ctatggtgat aaccagggcc atagaagacc ttttctctct
    10081 tacctttctc tgataggatc gtgtccttca gtcaagagct catgtgaact ccagacttta
    10141 tattacatct ctgaagttct tgatgtgggg aagattgact tttattccat ttttatatga
    10201 aggtgttaga tatagccatc aatttatttt tctacattcc ctctacgctt atgtaatttt
    10261 ctatttttaa atcttctctt aaatatactg agaatctcta gtctcctttt ctaaaggctg
    10321 atccaactac acttgtgggc tatttttcca agctttgtgt gaaacttgat gacattgggc
    10381 cagcaaaatg caaagaagac tatagtttca ggcagaaaat cacgtccaaa gaatttggca
    10441 tggattaaac aacctctacc acactatagc tctcacatag gctgaacatt ttcctaaatt
    10501 ctacctgtgc agacactgag gggctctcta cctttgaaac tatgagaacg tcttaaatta
    10561 atatgaatat ttctctccat gtatagtgtg agctgccaat gcattatctt aatacatcaa
    10621 gaaggaaatc agatttcagg cacacattag caattgtttg ttaatgtcct tggcaaacgt
    10681 gtacctgctt tccttcattg ttctcttcag gggctttctt ccactttcct cttatgaaac
    10741 gaatgtgttt ttgactgtta actgggttcg ctttccagat ctgtctttcc caaatgaaag
    10801 gttagtccca tagaccactg gctattcagg gaaattactc tctacctccc catgacatgg
    10861 atgataagtg ttgagggatg aacactctgg aaactcttag catggatttt gaattccaga
    10921 tttctttata ggagatgtat aaaaaggagt ttatagcata caaaaattat actgctcttc
    10981 ccagtaaggg actaaaagga catttgaaat ctttacattt tagatgtttt tgtgaattat
    11041 aaatatctcc tttttcctcg cttcatgcta acttgtctct agataaaatc ttatttcttc
    11101 atacattgga ccacaaggca taaagaaggt agctcagcgc cactgagatt gtgttctgca
    11161 taatatttgg aaggcttcca tttccattga aaacaaatcc atgagtgaga gggaaagtct
    11221 agtatcaatt cttctgtttg ctcgcaatga cacaaatagg ttttggggat ctacctaagg
    11281 gataggtcta ctccaaaatt attaaattta taattgtgca attctgtaat ttcccaaggc
    11341 ataagtaata tgaccctact gtcagctaga tgtcttatct tcaaagagag tatgcattaa
    11401 taaaaagaac ttccctttaa agaaccaatc taaaatacta aaaaggcaga aacttttaaa
    11461 attagaaatt ggataatttt taagaatctt tagagaaaac attggtttat catagtcttt
    11521 ttctttcatt gagttttcat ttagactagc atggcaagca gggtggcctt ggactttgat
    11581 ttaggggatc gtgctttggc ctggaaaata agcactggcc tgcatgccta gaacctgagt
    11641 gaaggcagtc aacatcctag tatgaatcag acctagcaga aatgtaaatt atttcaagta
    11701 cttcagggtt tagttttctt agtgacaccc tggaccctgg atgctgcttt ctaaagtgca
    11761 cctgatccat gcagttttat gtcttcatat aactggtatc ttgtgagttt gcaagcaatg
    11821 ggatgagcaa gaaaaaaagg acgcatggag gagaaaaatt tgaacgaggc tagtgcaatg
    11881 ttttgctgat agattacatt gttagggagc tggtgatttt ttgccatggt cgtatcgtgg
    11941 acagcttttc cgtggaaatt caaggcatct gtattaggca aatgtgagtg cccttgatct
    12001 tgtattgatc agtgccaatg tactgggaaa gcaggtaccc cagtgaaact ggtctgtgct
    12061 tggtttaaca gctccaacaa tttcagatcc atggggctgc ttgaccatag accctgttta
    12121 ctccatgcct gttcaaaagt cattttgact tctagctttg tttcactttc ttctttcaat
    12181 acttctgttt cctcgtcctt ttccttcata ttccatggct atttctttcc tactttacag
    12241 tttcccccag acccagattt tttcacacct ctgcatcata gacaactgag tagctccctt
    12301 ggcccctcct ccatcctctc acaccctgtg ctccgttcct cagccgtttg gacagggagc
    12361 tccgctgtca ccaaggagcc catgtgagac acactgctgt gactgcctct tgttaatgtc
    12421 agcatcacct catcacttaa gcaaaaagga aaatccataa aagagatgga aaatacgtct
    12481 ctttttattt tattttaaga agattgggcg gggtgggggg ggggttctta ttctaggcct
    12541 tctcaatttc tcatagattt taccttaaac taattaaggc cattctcttg ataaatttgt
    12601 acatcagacc gggtcaccag ctatgatgca gaacgccaga tttttgaatc tcaagtaact
    12661 ttctgtggtg ctggacaaat tgacttaatt ctgtgcaggc aaacactttg aatcataagg
    12721 atttttattg ccgctccatt cttactacta ttcatgattc aaacatctac ccctgttctg
    12781 aatcaggatg ttgacacttc ttggttattt tcagatgaac agtaactgct acactcttga
    12841 aagcacttaa aagtgcaagc atgtcctaaa tagccattta acctggtaaa acataggctt
    12901 ttctgtttaa ttaagtatta gaccagtctg tagatataat ctgaaaagat tgtaggcagt
    12961 aatgaagaca gttggggaaa ggagaaggcc ctttaaagac atgaaacctt acacgctctt
    13021 gggatatttt taagcataat aagctcattg gattcaggta tttttccctt tgcattttta
    13081 aaaatacgta tttctaattt gtttgcatat ttaattttgt caaagctgag aaatgctcat
    13141 gagttgaatt tataaatgtc atttgcaacc aaatgaagta tttattttta aaaagagagt
    13201 gaaggaacca acactgattt gtacataata aaaatgtgtg tattatatat atatattttt
    13261 tcctccttga cagtacttgg tcacaatatc aagtgtattt ttgtacataa tatatattga
    13321 ttagaaaaac gtcaattgtc tattcaaaaa attctatctc tgtgatagat tatatttatc
    13381 ctaatctgtt gatacctctg ttaatttgtt ttaagagaat tatatttttt ggaatttaca
    13441 gagaattgca ttcatggctt tcaattgtaa atatgctaag ggtattttaa taaatcttgg
    13501 tttcatgtcc a
//
