LOCUS       NM_001308068            5043 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens FLYWCH-type zinc finger 1 (FLYWCH1), transcript
            variant 3, mRNA.
ACCESSION   NM_001308068 XM_005255628
VERSION     NM_001308068.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Santos-Barriopedro,I., van Mierlo,G. and Vermeulen,M.
  TITLE     Off-the-shelf proximity biotinylation for interaction proteomics
  JOURNAL   Nat Commun 12 (1), 5015 (2021)
   PUBMED   34408139
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Almozyan,S., Coulton,J., Babaei-Jadidi,R. and Nateri,A.S.
  TITLE     FLYWCH1, a Multi-Functional Zinc Finger Protein Contributes to the
            DNA Repair Pathway
  JOURNAL   Cells 10 (4), 889 (2021)
   PUBMED   33924684
  REMARK    GeneRIF: FLYWCH1, a Multi-Functional Zinc Finger Protein
            Contributes to the DNA Repair Pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5043)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 5043)
  AUTHORS   Almars,A., Chondrou,P.S., Onyido,E.K., Almozyan,S., Seedhouse,C.,
            Babaei-Jadidi,R. and Nateri,A.S.
  TITLE     Increased FLYWCH1 Expression is Negatively Correlated with
            Wnt/beta-catenin Target Gene Expression in Acute Myeloid Leukemia
            Cells
  JOURNAL   Int J Mol Sci 20 (11), 2739 (2019)
   PUBMED   31167387
  REMARK    GeneRIF: we identify for the first time FLYWCH1 as a potential
            negative regulator of the downstream targets of the
            Wnt/beta-catenin pathway in AML cells. Hence, FLYWCH1 might be an
            intrinsic inhibitory factor of the oncogenic nuclear beta-catenin
            in AML cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5043)
  AUTHORS   Muhammad,B.A., Almozyan,S., Babaei-Jadidi,R., Onyido,E.K.,
            Saadeddin,A., Kashfi,S.H., Spencer-Dene,B., Ilyas,M.,
            Lourdusamy,A., Behrens,A. and Nateri,A.S.
  TITLE     FLYWCH1, a Novel Suppressor of Nuclear beta-Catenin, Regulates
            Migration and Morphology in Colorectal Cancer
  JOURNAL   Mol Cancer Res 16 (12), 1977-1990 (2018)
   PUBMED   30097457
  REMARK    GeneRIF: Study uncovers a new molecular mechanism by which FLYWCH1
            with a possible tumor suppressive role represses
            beta-catenin-induced ZEB1 and increases cadherin-mediated cell
            attachment preventing colorectal cancer metastasis.
REFERENCE   6  (bases 1 to 5043)
  AUTHORS   Haskell,G.T., Jensen,B.C., Skrzynia,C., Pulikkotil,T., Tilley,C.R.,
            Lu,Y., Marchuk,D.S., Ann Samsa,L., Wilhelmsen,K.C., Lange,E.,
            Patterson,C., Evans,J.P. and Berg,J.S.
  TITLE     Genetic Complexity of Mitral Valve Prolapse Revealed by Clinical
            and Genetic Evaluation of a Large Family
  JOURNAL   J Heart Valve Dis 26 (5), 569-580 (2017)
   PUBMED   29762926
  REMARK    GeneRIF: FLYWCH1 variant of uncertain significance in candidate
            gene was identified in Familial Mitral Valve Prolapse.
REFERENCE   7  (bases 1 to 5043)
  AUTHORS   Foroughi Asl,H., Talukdar,H.A., Kindt,A.S., Jain,R.K., Ermel,R.,
            Ruusalepp,A., Nguyen,K.D., Dobrin,R., Reilly,D.F., Schunkert,H.,
            Samani,N.J., Braenne,I., Erdmann,J., Melander,O., Qi,J., Ivert,T.,
            Skogsberg,J., Schadt,E.E., Michoel,T. and Bjorkegren,J.L.
  CONSRTM   CARDIoGRAM Consortium
  TITLE     Expression quantitative trait Loci acting across multiple tissues
            are enriched in inherited risk for coronary artery disease
  JOURNAL   Circ Cardiovasc Genet 8 (2), 305-315 (2015)
   PUBMED   25578447
  REMARK    GeneRIF: eQTLs acting across multiple tissues are significant
            carriers of inherited risk for CAD. FLYWCH1, PSORSIC3, and G3BP1
            are novel master regulatory genes in CAD that may be suitable
            targets.
REFERENCE   8  (bases 1 to 5043)
  AUTHORS   Krauss,V. and Dorn,R.
  TITLE     Evolution of the trans-splicing Drosophila locus mod(mdg4) in
            several species of Diptera and Lepidoptera
  JOURNAL   Gene 331, 165-176 (2004)
   PUBMED   15094203
  REMARK    GeneRIF: Identifies the human FLYWCH1 protein (AB046772/KIAA1552)
            as containing five FLYWCH domain copies.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC004234.1, AC004034.2 and AC004235.1.
            
            On Feb 27, 2020 this sequence version replaced NM_001308068.1.
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the 3' coding region compared to variant 1. The encoded isoform
            (c) is longer than isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028572.1, SRR14038193.1172320.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253928.14/ ENSP00000253928.9
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-224               AC004234.1         39486-39709
            225-338             AC004034.2         33252-33365         c
            339-736             AC004034.2         17531-17928         c
            737-1207            AC004034.2         16661-17131         c
            1208-1660           AC004034.2         13959-14411         c
            1661-1924           AC004034.2         13562-13825         c
            1925-2188           AC004034.2         10157-10420         c
            2189-2461           AC004034.2         9085-9357           c
            2462-2522           AC004034.2         7449-7509           c
            2523-5043           AC004235.1         4638-7158
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..5043
                     /gene="FLYWCH1"
                     /note="FLYWCH-type zinc finger 1"
                     /db_xref="GeneID:84256"
                     /db_xref="HGNC:HGNC:25404"
                     /db_xref="MIM:620431"
     exon            1..224
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            225..338
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            339..736
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             412..2562
                     /gene="FLYWCH1"
                     /note="isoform c is encoded by transcript variant 3;
                     FLYWCH-type zinc finger-containing protein 1"
                     /codon_start=1
                     /product="FLYWCH-type zinc finger-containing protein 1
                     isoform c"
                     /protein_id="NP_001294997.1"
                     /db_xref="CCDS:CCDS76809.1"
                     /db_xref="GeneID:84256"
                     /db_xref="HGNC:HGNC:25404"
                     /db_xref="MIM:620431"
                     /translation="MPLPEPSEQEGESVKAGQEPSPKPGTDVIPAAPRKPREFSKLVL
                     LTASDQDEDGVGSKPQEVHCVLSLEMAGPATLASTLQILPVEEQGGVVQPALEMPEQK
                     CSKLDAAAPQSLEFLRTPFGGRLLVLESFLYKQEKAVGDKVYWKCRQHAELGCRGRAI
                     TRGLRATVMRGHCHAPDEQGLEARRQREKLPSLALPEGLGEPQGPEGPGGRVEEPLEG
                     VGPWQCPEEPEPTPGLVLSKPALEEEEAPRALSLLSLPPKKRSILGLGQARPLEFLRT
                     CYGGSFLVHESFLYKREKAVGDKVYWTCRDHALHGCRSRAITQGQRVTVMRGHCHQPD
                     MEGLEARRQQEKAVETLQAGQDGPGSQVDTLLRGVDSLLYRRGPGPLTLTRPRPRKRA
                     KVEDQELPTQPEAPDEHQDMDADPGGPEFLKTPLGGSFLVYESFLYRREKAAGEKVYW
                     TCRDQARMGCRSRAITQGRRVTVMRGHCHPPDLGGLEALRQREKRPNTAQRGSPGGPE
                     FLKTPLGGSFLVYESFLYRREKAAGEKVYWTCRDQARMGCRSRAITQGRRVMVMRRHC
                     HPPDLGGLEALRQREHFPNLAQWDSPDPLRPLEFLRTSLGGRFLVHESFLYRKEKAAG
                     EKVYWMCRDQARLGCRSRAITQGHRIMVMRSHCHQPDLAGLEALRQRERLPTTAQQED
                     PEKIQVQLCFKTCSPESQQIYGDIKDVRLDGESQ"
     exon            737..1207
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1660
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1924
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2188
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2461
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2522
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..5043
                     /gene="FLYWCH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3625..3630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FLYWCH1"
                     /note="hexamer: AATAAA"
     polyA_site      3647
                     /gene="FLYWCH1"
                     /note="major polyA site"
     regulatory      5016..5021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FLYWCH1"
                     /note="hexamer: AATAAA"
     polyA_site      5043
                     /gene="FLYWCH1"
ORIGIN      
        1 gaagtagcgc gcctgagcgt tccgcaaggc cggctccccg gcggggtcgc gcgcgcggtc
       61 acggggctcg ctcccgaggg gcaggtcggg gctgggagct ggtgcccggg tcggggtggc
      121 ggcggcggcc tgggtcgcgg ggtcgacgct cgctgctgca gcggcagagg ctgaaggatc
      181 cgccgcggcg ctgtcgcggg agagggaggg ccccgctgcc gtcggtgaaa ccagcccaga
      241 gaagttacgg gatttgcccc cggggctcct gcccagcaag ccagcgccgt gacccaggtg
      301 tgggggatga tggtgacgct tctcactcac gtgtgaagga cggaaccact gcactccagg
      361 ttccttgctg ggtgctgagc gtggcctgag ggacaggccc tgggtcccgg gatgcccctg
      421 cccgagccca gcgagcagga gggcgagagt gtgaaggccg gccaggagcc atcccccaag
      481 ccaggcacgg acgtcatccc ggcagccccc aggaagccca gggagttctc caaactggtg
      541 ctgctcacag cctccgacca agatgaggat ggggtgggat ccaagcccca ggaagtgcac
      601 tgcgtcctgt ccctggagat ggctggcccc gccaccctcg ccagcacctt gcagatcctg
      661 ccagttgagg agcagggagg ggtggtccag ccagccctag agatgcctga acagaagtgc
      721 agcaagctgg atgcagcagc ccctcagtcc ctggagttcc tgaggacacc attcgggggc
      781 cgcctcctgg tgctggagtc cttcctgtac aagcaggaga aggcagtggg ggacaaggtg
      841 tactggaagt gccgccaaca tgctgagctg ggctgccggg gccgggccat cacccgaggc
      901 ctgcgggcca cagtgatgcg gggccactgc cacgcgcccg atgagcaagg cctggaggcc
      961 cggcgccaga gggagaaact gcccagcctg gccctgccag agggcttggg agagccccag
     1021 ggtcctgagg gccctggagg ccgagtggag gagcccctgg agggggtggg cccgtggcag
     1081 tgccctgagg agcccgagcc cactcctggg ctggtgctga gcaagccggc cctggaggag
     1141 gaggaggcac cccgagccct gtcactgctg agcctgccgc ccaagaagcg ctcgatcctg
     1201 gggctgggac aggcccggcc cctcgagttc ctgaggacgt gctacggggg cagcttcctg
     1261 gtacacgagt cgttcctcta caagcgggag aaggctgtcg gggacaaggt gtattggacc
     1321 tgccgggacc acgcgctgca cggctgccgg agccgggcca tcacccaggg acagcgggtg
     1381 actgtgatgc gtgggcactg ccaccagccc gatatggagg gcctggaagc ccggcggcag
     1441 caggagaagg ccgtggagac gctgcaggct gggcaggacg gccctgggag ccaagtggac
     1501 acgctgctcc gaggcgtgga tagtttgctc taccgcaggg gtccgggtcc cctgactctc
     1561 accaggcctc ggcccagaaa gcgagcaaag gtcgaagacc aggagctgcc aacccagccc
     1621 gaggccccag acgagcacca ggacatggac gcagacccgg gaggccctga gttcctgaag
     1681 acgcccctgg ggggcagctt cctggtgtac gagtccttcc tctaccggcg ggagaaggcg
     1741 gctggggaga aggtgtattg gacctgccgg gaccaggccc gcatgggctg ccgcagccgc
     1801 gccatcaccc agggccgacg ggtgactgtc atgcgtggtc actgccaccc gcccgacctg
     1861 ggaggcctgg aggccctgag gcagcgggag aaacgcccca acacggcgca gcgggggagc
     1921 ccaggaggcc ccgagttcct gaagacgccc ctggggggca gcttcctggt gtacgagtcc
     1981 ttcctctacc ggcgggagaa ggcggccggg gagaaggtgt attggacctg ccgggaccag
     2041 gcccgcatgg gctgccgcag ccgcgccatc acccagggcc ggcgggtcat ggtcatgcgc
     2101 aggcactgcc acccaccgga cctgggcggc ctggaggccc tgcggcagcg ggagcacttc
     2161 cccaacctgg cgcagtggga cagcccagat cctctccggc ccctggagtt cctgaggact
     2221 tccctggggg gcaggttcct ggtgcacgag tccttcctct acaggaagga gaaggcggct
     2281 ggggagaagg tgtactggat gtgccgggac caggctcggc tgggctgccg cagccgcgcc
     2341 ataacccagg gccaccgcat catggtcatg cgcagccact gccatcagcc tgacctggca
     2401 ggcctggagg ccttgaggca acgggagcgg ctccccacca cggcccagca ggaggaccca
     2461 gaaaagattc aagttcagct gtgcttcaag acgtgttctc ctgaaagcca gcagatttat
     2521 ggggacatca aagacgtcag actggatggc gagtcccagt gaggcgatgt gggcagagga
     2581 gctccgagcc gcccacccaa ggtggcttca catccacaca ggcacttccc atccacctag
     2641 gtttggctta gcagaaactt cttttcattc ttccaaagca tcgatggtct tcgcgtctcc
     2701 tcaggaggtc tcccaggagg aattcttgga tggtgtcctc atgtcggcgg agaacagtgc
     2761 tcagagctgg cgcttgcaga cgcagctgtc gtggggcagg gcggtggcgc cttcctgacc
     2821 tttggaagac atgacaaagc tgcctggaca cggacgcccc tgctgtacgg ccacagcacc
     2881 cctgggtttg cagagcacgc agccttccta gggctttcca cctggcgagg ccccgctctg
     2941 ctcagcacgg tgcaaagtga atgctgctgt cttggagcct gggcacgttt ggggaagttc
     3001 ctgcttcaaa ctgagctgcc ccgcataggc caggtcaacc cacaccaatc tcttctggac
     3061 aggtgctggg taggccttcc tggtctctgg ccgcctgctg ccagggtgtg gccatcccca
     3121 gcaaccggag ctggccaaac cagaggcctc gctccgcact ccacactttc ctttctgtgc
     3181 tccttccaag ttaaattaaa ccccctctcc acgattccca cggcaggcgt cattcccgag
     3241 atgggagcca gtccaggggt cagcaggagc cagcgctggg cacacgtgcc ctggctgagg
     3301 ccagcggcat cctgggtggc ccaggtccat cctgggcagc aaaggcgtgt ccccttctgt
     3361 cagacagctt cacagagtgt ggcttcacca gtcagaggga gcagtccgga gaggcaagat
     3421 gaccccaccg ggactgcaga gcctcctcct tactaacaag gacctgtccg cagccgcgag
     3481 gtccttcact cccaccctgt aattgtgggg ggagtgccag caacaggcct gtcccctggc
     3541 aagttggcca cggaacccac catgcactgc aaggctgtga cagcctgggc acccctgctt
     3601 ctcctctgct tgtacggttc ccccaataaa tcctattttc catctcaccg tgtggagctg
     3661 gtacttgttc acatccctct tgcagcaaca gttggccgcc gtgaaaccac actccgtcac
     3721 atctccatgc cagtgcccag ggggagaccc ctccccatgc ctgggctgtt acagtcacct
     3781 ggcccagacg taacctggac caagagtgga cggagacacc tggggttggg gcctctggaa
     3841 gggctgctgg ggggccagct gcagcctgtg gcctctccca ggcccggata tggcaggaca
     3901 tttgcgtcgt gttgctggaa gaggcaggat gctctgttct cgccattagc ctgcgttacg
     3961 cctagactca tctgcataag cctgggcaag tggcacccct gggccaaggg cagacttgcc
     4021 cacccgtcca tcctttgcct atggcagagg acacacaaca cagtggctgc gctttgggca
     4081 ctgttctcgg ccctgaagca tcctgccccg aggctgacct aactgccccc agctggactc
     4141 atggccaagg ctacagaccc agtactccaa tcccgactcc cactgtgtgg tccaaatgtg
     4201 tacgcccaca ggaggcagcc atgcccgagg aaggtacttg ctgaaagatg gtccagttca
     4261 tgccgccacc agctggacct ctgatcaggc cccctgcaga aggggaagct ctccacatgc
     4321 ccaagatacg agagaaacgc ccgggtcaca gcagcctgag tcactgcagc ctctgctacc
     4381 tgccaagcct gcgccctcca cctgtgggct ccttgcctct gtgatggggc ctccacggcc
     4441 agcatcttcc cgccatggtc acatgatgcc atgccctgct ctgggcaccc ctaagcgggt
     4501 atgaggcgaa tcagaggaag aatatcggct tctcttccct tttggatatt tgcgtcattg
     4561 accgggactg gcccactctt cctctgcctc acgcctgctt tgaccttaga agacagacgg
     4621 cagctcgcgt tgctgggctg agattctcca atggacgtga atttagatga tgctgttgcc
     4681 ttgggtggct tcatggccac caacccgcac cctggtggcc actcagccat gacaagggcc
     4741 tgacagccac taagtgagct tcagagcctt ccccttgcaa agttccaggg ccaccagatg
     4801 tcgcctgccc agtcactgtc acagagttaa agcgtgcggg tgaacgctgg ccgcggagcg
     4861 tgcagcccgg cagcagctca gcggggcagc tcctgcttcc agccctgcct ctgctggtgc
     4921 ttcgcccaca aaccctgctg tatgacattt gcaccacatg gtgtgtgtcc gtgccctttg
     4981 acatgacagt gccaacaaca tctcatgaaa tttaaaataa atccccaagg catcagaaac
     5041 gta
//
