LOCUS       NM_002019               7123 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens fms related receptor tyrosine kinase 1 (FLT1),
            transcript variant 1, mRNA.
ACCESSION   NM_002019
VERSION     NM_002019.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Figueroa,L., Rosas,M., Alvarez,M., Aguilar,E., Mateu,V. and
            Bonilla,E.
  TITLE     Interaction of Purine and its Derivatives with A1, A2-Adenosine
            Receptors and Vascular Endothelial Growth Factor Receptor-1
            (Vegf-R1) as a Therapeutic Alternative to Treat Cancer
  JOURNAL   Drug Res (Stuttg) 74 (8), 379-393 (2024)
   PUBMED   39173673
  REMARK    GeneRIF: Interaction of Purine and its Derivatives with A1,
            A2-Adenosine Receptors and Vascular Endothelial Growth Factor
            Receptor-1 (Vegf-R1) as a Therapeutic Alternative to Treat Cancer.
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Tai,Y., Chow,A., Han,S., Coker,C., Ma,W., Gu,Y., Estrada
            Navarro,V., Kandpal,M., Hibshoosh,H., Kalinsky,K.,
            Manova-Todorova,K., Safonov,A., Walsh,E.M., Robson,M., Norton,L.,
            Baer,R., Merghoub,T., Biswas,A.K. and Acharyya,S.
  TITLE     FLT1 activation in cancer cells promotes PARP-inhibitor resistance
            in breast cancer
  JOURNAL   EMBO Mol Med 16 (8), 1957-1980 (2024)
   PUBMED   38956205
  REMARK    GeneRIF: FLT1 activation in cancer cells promotes PARP-inhibitor
            resistance in breast cancer.
REFERENCE   3  (bases 1 to 7123)
  AUTHORS   Vogtmann,R., Riedel,A., Sassmannshausen,I., Langer,S.,
            Kuhnel-Terjung,E., Kimmig,R., Schorle,H., Winterhager,E. and
            Gellhaus,A.
  TITLE     Overexpression of Human sFLT1 in the Spongiotrophoblast Is
            Sufficient to Induce Placental Dysfunction and Fetal Growth
            Restriction in Transgenic Mice
  JOURNAL   Int J Mol Sci 25 (4), 2040 (2024)
   PUBMED   38396719
  REMARK    GeneRIF: Overexpression of Human sFLT1 in the Spongiotrophoblast Is
            Sufficient to Induce Placental Dysfunction and Fetal Growth
            Restriction in Transgenic Mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7123)
  AUTHORS   Jin,P., Zhang,J., Sumariwalla,P.F., Ni,I., Jorgensen,B.,
            Crawford,D., Phillips,S., Feldmann,M., Shepard,H.M. and
            Paleolog,E.M.
  TITLE     Novel splice variants derived from the receptor tyrosine kinase
            superfamily are potential therapeutics for rheumatoid arthritis
  JOURNAL   Arthritis Res Ther 10 (4), R73 (2008)
   PUBMED   18593464
REFERENCE   5  (bases 1 to 7123)
  AUTHORS   Cunningham,S.A., Waxham,M.N., Arrate,P.M. and Brock,T.A.
  TITLE     Interaction of the Flt-1 tyrosine kinase receptor with the p85
            subunit of phosphatidylinositol 3-kinase. Mapping of a novel site
            involved in binding
  JOURNAL   J Biol Chem 270 (35), 20254-20257 (1995)
   PUBMED   7657594
REFERENCE   6  (bases 1 to 7123)
  AUTHORS   Kendall,R.L. and Thomas,K.A.
  TITLE     Inhibition of vascular endothelial cell growth factor activity by
            an endogenously encoded soluble receptor
  JOURNAL   Proc Natl Acad Sci U S A 90 (22), 10705-10709 (1993)
   PUBMED   8248162
REFERENCE   7  (bases 1 to 7123)
  AUTHORS   de Vries,C., Escobedo,J.A., Ueno,H., Houck,K., Ferrara,N. and
            Williams,L.T.
  TITLE     The fms-like tyrosine kinase, a receptor for vascular endothelial
            growth factor
  JOURNAL   Science 255 (5047), 989-991 (1992)
   PUBMED   1312256
REFERENCE   8  (bases 1 to 7123)
  AUTHORS   Shibuya,M., Yamaguchi,S., Yamane,A., Ikeda,T., Tojo,A.,
            Matsushime,H. and Sato,M.
  TITLE     Nucleotide sequence and expression of a novel human receptor-type
            tyrosine kinase gene (flt) closely related to the fms family
  JOURNAL   Oncogene 5 (4), 519-524 (1990)
   PUBMED   2158038
REFERENCE   9  (bases 1 to 7123)
  AUTHORS   Satoh,H., Yoshida,M.C., Matsushime,H., Shibuya,M. and Sasaki,M.
  TITLE     Regional localization of the human c-ros-1 on 6q22 and flt on 13q12
  JOURNAL   Jpn J Cancer Res 78 (8), 772-775 (1987)
   PUBMED   3115921
REFERENCE   10 (bases 1 to 7123)
  AUTHORS   Matsushime,H., Yoshida,M.C., Sasaki,M. and Shibuya,M.
  TITLE     A possible new member of tyrosine kinase family, human frt
            sequence, is highly conserved in vertebrates and located on human
            chromosome 13
  JOURNAL   Jpn J Cancer Res 78 (7), 655-661 (1987)
   PUBMED   3040650
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA851664.1, AF063657.2,
            BX504049.1, BU151621.1 and AL138712.19.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 9, 2009 this sequence version replaced NM_002019.3.
            
            Summary: This gene encodes a member of the vascular endothelial
            growth factor receptor (VEGFR) family. VEGFR family members are
            receptor tyrosine kinases (RTKs) which contain an extracellular
            ligand-binding region with seven immunoglobulin (Ig)-like domains,
            a transmembrane segment, and a tyrosine kinase (TK) domain within
            the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and
            placental growth factor and plays an important role in angiogenesis
            and vasculogenesis. Expression of this receptor is found in
            vascular endothelial cells, placental trophoblast cells and
            peripheral blood monocytes. Multiple transcript variants encoding
            different isoforms have been found for this gene. Isoforms include
            a full-length transmembrane receptor isoform and shortened, soluble
            isoforms. The soluble isoforms are associated with the onset of
            pre-eclampsia.[provided by RefSeq, May 2009].
            
            Transcript Variant: This variant (1) the longest isoform (1).
            Isoform 1 is a transmembrane protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.9362.1,
                                           SRR18074967.403229.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282397.9/ ENSP00000282397.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-592               DA851664.1         1-592
            593-1753            AF063657.2         308-1468
            1754-1869           BX504049.1         393-508
            1870-3923           AF063657.2         1585-3638
            3924-4100           BU151621.1         462-638
            4101-7123           AL138712.19        68059-71081         c
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.3"
     gene            1..7123
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /note="fms related receptor tyrosine kinase 1"
                     /db_xref="GeneID:2321"
                     /db_xref="HGNC:HGNC:3763"
                     /db_xref="MIM:165070"
     exon            1..349
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             286..4302
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /EC_number="2.7.10.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; vascular endothelial growth factor receptor 1;
                     fms-like tyrosine kinase 1; tyrosine-protein kinase FRT;
                     tyrosine-protein kinase receptor FLT; vascular
                     permeability factor receptor; fms-related tyrosine kinase
                     1 (vascular endothelial growth factor/vascular
                     permeability factor receptor); fms related tyrosine kinase
                     1"
                     /codon_start=1
                     /product="vascular endothelial growth factor receptor 1
                     isoform 1 precursor"
                     /protein_id="NP_002010.2"
                     /db_xref="CCDS:CCDS9330.1"
                     /db_xref="GeneID:2321"
                     /db_xref="HGNC:HGNC:3763"
                     /db_xref="MIM:165070"
                     /translation="MVSYWDTGVLLCALLSCLLLTGSSSGSKLKDPELSLKGTQHIMQ
                     AGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHT
                     GFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRV
                     TSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKT
                     NYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRA
                     SVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFI
                     TVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLII
                     KDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQ
                     ILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQR
                     MAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKM
                     PTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIM
                     NVSLQDSGTYACRARNVYTGEEILQKKEITIRDQEAPYLLRNLSDHTVAISSSTTLDC
                     HANGVPEPQITWFKNNHKIQQEPGIILGPGSSTLFIERVTEEDEGVYHCKATNQKGSV
                     ESSAYLTVQGTSDKSNLELITLTCTCVAATLFWLLLTLFIRKMKRSSSEIKTDYLSII
                     MDPDEVPLDEQCERLPYDASKWEFARERLKLGKSLGRGAFGKVVQASAFGIKKSPTCR
                     TVAVKMLKEGATASEYKALMTELKILTHIGHHLNVVNLLGACTKQGGPLMVIVEYCKY
                     GNLSNYLKSKRDLFFLNKDAALHMEPKKEKMEPGLEQGKKPRLDSVTSSESFASSGFQ
                     EDKSLSDVEEEEDSDGFYKEPITMEDLISYSFQVARGMEFLSSRKCIHRDLAARNILL
                     SENNVVKICDFGLARDIYKNPDYVRKGDTRLPLKWMAPESIFDKIYSTKSDVWSYGVL
                     LWEIFSLGGSPYPGVQMDEDFCSRLREGMRMRAPEYSTPEIYQIMLDCWHRDPKERPR
                     FAELVEKLGDLLQANVQQDGKDYIPINAILTGNSGFTYSTPAFSEDFFKESISAPKFN
                     SGSSDDVRYVNAFKFMSLERIKTFEELLPNATSMFDDYQGDSSTLLASPMLKRFTWTD
                     SKPKASLKIDLRVTSKSKESGLSDVSRPSFCHSSCGHVSEGKRRFTYDHAELERKIAC
                     CSPPPDYNSVVLYSTPPI"
     exon            350..446
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            447..673
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            674..798
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            799..961
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1098
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1273
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1391
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1561
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1721
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1836
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1945
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..2254
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2401
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2533
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2640
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2773
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2774..2878
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2879..2992
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2993..3081
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3238
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3239..3336
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3337..3459
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3571
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3572..3671
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3777
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3778..3920
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            3921..4005
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            4006..4100
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..7123
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7104..7109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /note="hexamer: AATAAA"
     polyA_site      7123
                     /gene="FLT1"
                     /gene_synonym="FLT; FLT-1; VEGFR-1; VEGFR1"
                     /note="major polyA site"
ORIGIN      
        1 atcgaggtcc gcgggaggct cggagcgcgc caggcggaca ctcctctcgg ctcctccccg
       61 gcagcggcgg cggctcggag cgggctccgg ggctcgggtg cagcggccag cgggcgcctg
      121 gcggcgagga ttacccgggg aagtggttgt ctcctggctg gagccgcgag acgggcgctc
      181 agggcgcggg gccggcggcg gcgaacgaga ggacggactc tggcggccgg gtcgttggcc
      241 gcggggagcg cgggcaccgg gcgagcaggc cgcgtcgcgc tcaccatggt cagctactgg
      301 gacaccgggg tcctgctgtg cgcgctgctc agctgtctgc ttctcacagg atctagttca
      361 ggttcaaaat taaaagatcc tgaactgagt ttaaaaggca cccagcacat catgcaagca
      421 ggccagacac tgcatctcca atgcaggggg gaagcagccc ataaatggtc tttgcctgaa
      481 atggtgagta aggaaagcga aaggctgagc ataactaaat ctgcctgtgg aagaaatggc
      541 aaacaattct gcagtacttt aaccttgaac acagctcaag caaaccacac tggcttctac
      601 agctgcaaat atctagctgt acctacttca aagaagaagg aaacagaatc tgcaatctat
      661 atatttatta gtgatacagg tagacctttc gtagagatgt acagtgaaat ccccgaaatt
      721 atacacatga ctgaaggaag ggagctcgtc attccctgcc gggttacgtc acctaacatc
      781 actgttactt taaaaaagtt tccacttgac actttgatcc ctgatggaaa acgcataatc
      841 tgggacagta gaaagggctt catcatatca aatgcaacgt acaaagaaat agggcttctg
      901 acctgtgaag caacagtcaa tgggcatttg tataagacaa actatctcac acatcgacaa
      961 accaatacaa tcatagatgt ccaaataagc acaccacgcc cagtcaaatt acttagaggc
     1021 catactcttg tcctcaattg tactgctacc actcccttga acacgagagt tcaaatgacc
     1081 tggagttacc ctgatgaaaa aaataagaga gcttccgtaa ggcgacgaat tgaccaaagc
     1141 aattcccatg ccaacatatt ctacagtgtt cttactattg acaaaatgca gaacaaagac
     1201 aaaggacttt atacttgtcg tgtaaggagt ggaccatcat tcaaatctgt taacacctca
     1261 gtgcatatat atgataaagc attcatcact gtgaaacatc gaaaacagca ggtgcttgaa
     1321 accgtagctg gcaagcggtc ttaccggctc tctatgaaag tgaaggcatt tccctcgccg
     1381 gaagttgtat ggttaaaaga tgggttacct gcgactgaga aatctgctcg ctatttgact
     1441 cgtggctact cgttaattat caaggacgta actgaagagg atgcagggaa ttatacaatc
     1501 ttgctgagca taaaacagtc aaatgtgttt aaaaacctca ctgccactct aattgtcaat
     1561 gtgaaacccc agatttacga aaaggccgtg tcatcgtttc cagacccggc tctctaccca
     1621 ctgggcagca gacaaatcct gacttgtacc gcatatggta tccctcaacc tacaatcaag
     1681 tggttctggc acccctgtaa ccataatcat tccgaagcaa ggtgtgactt ttgttccaat
     1741 aatgaagagt cctttatcct ggatgctgac agcaacatgg gaaacagaat tgagagcatc
     1801 actcagcgca tggcaataat agaaggaaag aataagatgg ctagcacctt ggttgtggct
     1861 gactctagaa tttctggaat ctacatttgc atagcttcca ataaagttgg gactgtggga
     1921 agaaacataa gcttttatat cacagatgtg ccaaatgggt ttcatgttaa cttggaaaaa
     1981 atgccgacgg aaggagagga cctgaaactg tcttgcacag ttaacaagtt cttatacaga
     2041 gacgttactt ggattttact gcggacagtt aataacagaa caatgcacta cagtattagc
     2101 aagcaaaaaa tggccatcac taaggagcac tccatcactc ttaatcttac catcatgaat
     2161 gtttccctgc aagattcagg cacctatgcc tgcagagcca ggaatgtata cacaggggaa
     2221 gaaatcctcc agaagaaaga aattacaatc agagatcagg aagcaccata cctcctgcga
     2281 aacctcagtg atcacacagt ggccatcagc agttccacca ctttagactg tcatgctaat
     2341 ggtgtccccg agcctcagat cacttggttt aaaaacaacc acaaaataca acaagagcct
     2401 ggaattattt taggaccagg aagcagcacg ctgtttattg aaagagtcac agaagaggat
     2461 gaaggtgtct atcactgcaa agccaccaac cagaagggct ctgtggaaag ttcagcatac
     2521 ctcactgttc aaggaacctc ggacaagtct aatctggagc tgatcactct aacatgcacc
     2581 tgtgtggctg cgactctctt ctggctccta ttaaccctct ttatccgaaa aatgaaaagg
     2641 tcttcttctg aaataaagac tgactaccta tcaattataa tggacccaga tgaagttcct
     2701 ttggatgagc agtgtgagcg gctcccttat gatgccagca agtgggagtt tgcccgggag
     2761 agacttaaac tgggcaaatc acttggaaga ggggcttttg gaaaagtggt tcaagcatca
     2821 gcatttggca ttaagaaatc acctacgtgc cggactgtgg ctgtgaaaat gctgaaagag
     2881 ggggccacgg ccagcgagta caaagctctg atgactgagc taaaaatctt gacccacatt
     2941 ggccaccatc tgaacgtggt taacctgctg ggagcctgca ccaagcaagg agggcctctg
     3001 atggtgattg ttgaatactg caaatatgga aatctctcca actacctcaa gagcaaacgt
     3061 gacttatttt ttctcaacaa ggatgcagca ctacacatgg agcctaagaa agaaaaaatg
     3121 gagccaggcc tggaacaagg caagaaacca agactagata gcgtcaccag cagcgaaagc
     3181 tttgcgagct ccggctttca ggaagataaa agtctgagtg atgttgagga agaggaggat
     3241 tctgacggtt tctacaagga gcccatcact atggaagatc tgatttctta cagttttcaa
     3301 gtggccagag gcatggagtt cctgtcttcc agaaagtgca ttcatcggga cctggcagcg
     3361 agaaacattc ttttatctga gaacaacgtg gtgaagattt gtgattttgg ccttgcccgg
     3421 gatatttata agaaccccga ttatgtgaga aaaggagata ctcgacttcc tctgaaatgg
     3481 atggctcctg aatctatctt tgacaaaatc tacagcacca agagcgacgt gtggtcttac
     3541 ggagtattgc tgtgggaaat cttctcctta ggtgggtctc catacccagg agtacaaatg
     3601 gatgaggact tttgcagtcg cctgagggaa ggcatgagga tgagagctcc tgagtactct
     3661 actcctgaaa tctatcagat catgctggac tgctggcaca gagacccaaa agaaaggcca
     3721 agatttgcag aacttgtgga aaaactaggt gatttgcttc aagcaaatgt acaacaggat
     3781 ggtaaagact acatcccaat caatgccata ctgacaggaa atagtgggtt tacatactca
     3841 actcctgcct tctctgagga cttcttcaag gaaagtattt cagctccgaa gtttaattca
     3901 ggaagctctg atgatgtcag atacgtaaat gctttcaagt tcatgagcct ggaaagaatc
     3961 aaaacctttg aagaactttt accgaatgcc acctccatgt ttgatgacta ccagggcgac
     4021 agcagcactc tgttggcctc tcccatgctg aagcgcttca cctggactga cagcaaaccc
     4081 aaggcctcgc tcaagattga cttgagagta accagtaaaa gtaaggagtc ggggctgtct
     4141 gatgtcagca ggcccagttt ctgccattcc agctgtgggc acgtcagcga aggcaagcgc
     4201 aggttcacct acgaccacgc tgagctggaa aggaaaatcg cgtgctgctc cccgccccca
     4261 gactacaact cggtggtcct gtactccacc ccacccatct agagtttgac acgaagcctt
     4321 atttctagaa gcacatgtgt atttataccc ccaggaaact agcttttgcc agtattatgc
     4381 atatataagt ttacaccttt atctttccat gggagccagc tgctttttgt gattttttta
     4441 atagtgcttt tttttttttg actaacaaga atgtaactcc agatagagaa atagtgacaa
     4501 gtgaagaaca ctactgctaa atcctcatgt tactcagtgt tagagaaatc cttcctaaac
     4561 ccaatgactt ccctgctcca acccccgcca cctcagggca cgcaggacca gtttgattga
     4621 ggagctgcac tgatcaccca atgcatcacg taccccactg ggccagccct gcagcccaaa
     4681 acccagggca acaagcccgt tagccccagg gatcactggc tggcctgagc aacatctcgg
     4741 gagtcctcta gcaggcctaa gacatgtgag gaggaaaagg aaaaaaagca aaaagcaagg
     4801 gagaaaagag aaaccgggag aaggcatgag aaagaatttg agacgcacca tgtgggcacg
     4861 gagggggacg gggctcagca atgccatttc agtggcttcc cagctctgac ccttctacat
     4921 ttgagggccc agccaggagc agatggacag cgatgagggg acattttctg gattctggga
     4981 ggcaagaaaa ggacaaatat cttttttgga actaaagcaa attttagaac tttacctatg
     5041 gaagtggttc tatgtccatt ctcattcgtg gcatgttttg atttgtagca ctgagggtgg
     5101 cactcaactc tgagcccata cttttggctc ctctagtaag atgcactgaa aacttagcca
     5161 gagttaggtt gtctccaggc catgatggcc ttacactgaa aatgtcacat tctattttgg
     5221 gtattaatat atagtccaga cacttaactc aatttcttgg tattattctg ttttgcacag
     5281 ttagttgtga aagaaagctg agaagaatga aaatgcagtc ctgaggagag gagttttctc
     5341 catatcaaaa cgagggctga tggaggaaaa aggtcaataa ggtcaaggga aaaccccgtc
     5401 tctataccaa ccaaaccaat tcaccaacac agttgggacc caaaacacag gaagtcagtc
     5461 acgtttcctt ttcatttaat ggggattcca ctatctcaca ctaatctgaa aggatgtgga
     5521 agagcattag ctggcgcata ttaagcactt taagctcctt gagtaaaaag gtggtatgta
     5581 atttatgcaa ggtatttctc cagttgggac tcaggatatt agttaatgag ccatcactag
     5641 aagaaaagcc cattttcaac tgctttgaaa cttgcctggg gtctgagcat gatgggaata
     5701 gggagacagg gtaggaaagg gcgcctactc ttcagggtct aaagatcaag tgggccttgg
     5761 atcgctaagc tggctctgtt tgatgctatt tatgcaagtt agggtctatg tatttatgat
     5821 gtctgcacct tctgcagcca gtcagaagct ggagaggcaa cagtggattg ctgcttcttg
     5881 gggagaagag tatgcttcct tttatccatg taatttaact gtagaacctg agctctaagt
     5941 aaccgaagaa tgtatgcctc tgttcttatg tgccacatcc ttgtttaaag gctctctgta
     6001 tgaagagatg ggaccgtcat cagcacattc cctagtgagc ctactggctc ctggcagcgg
     6061 cttttgtgga agactcacta gccagaagag aggagtggga cagtcctctc caccaagatc
     6121 taaatccaaa caaaagcagg ctagagccag aagagaggac aaatctttgt tcttcctctt
     6181 ctttacatac gcaaaccacc tgtgacagct ggcaatttta taaatcaggt aactggaagg
     6241 aggttaaaca cagaaaaaag aagacctcag tcaattctct actttttttt ttttttccaa
     6301 atcagataat agcccagcaa atagtgataa caaataaaac cttagctatt catgtcttga
     6361 tttcaataat taattcttaa tcattaagag accataataa atactccttt tcaagagaaa
     6421 agcaaaacca ttagaattgt tactcagctc cttcaaactc aggtttgtag catacatgag
     6481 tccatccatc agtcaaagaa tggttccatc tggagtctta atgtagaaag aaaaatggag
     6541 acttgtaata atgagctagt tacaaagtgc ttgttcatta aaatagcact gaaaattgaa
     6601 acatgaatta actgataata ttccaatcat ttgccattta tgacaaaaat ggttggcact
     6661 aacaaagaac gagcacttcc tttcagagtt tctgagataa tgtacgtgga acagtctggg
     6721 tggaatgggg ctgaaaccat gtgcaagtct gtgtcttgtc agtccaagaa gtgacaccga
     6781 gatgttaatt ttagggaccc gtgccttgtt tcctagccca caagaatgca aacatcaaac
     6841 agatactcgc tagcctcatt taaattgatt aaaggaggag tgcatctttg gccgacagtg
     6901 gtgtaactgt atgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgggt gtatgtgtgt
     6961 tttgtgcata actatttaag gaaactggaa ttttaaagtt acttttatac aaaccaagaa
     7021 tatatgctac agatataaga cagacatggt ttggtcctat atttctagtc atgatgaatg
     7081 tattttgtat accatcttca tataataaac ttccaaaaac aca
//
